LOCUS       DANJMA010000001       134496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000001 DANJMA010000000
VERSION     DANJMA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 134496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 134496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..134496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            598..1287
                     /locus_tag="PWU31_000001"
     CDS             598..1287
                     /locus_tag="PWU31_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1082581.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="PWU31_000002"
     CDS             1274..2482
                     /locus_tag="PWU31_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1082582.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="PWU31_000003"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="PWU31_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL1082583.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PWU31_000004"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PWU31_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL1082584.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="PWU31_000005"
     CDS             complement(6585..7280)
                     /locus_tag="PWU31_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL1082585.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PWU31_000006"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PWU31_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL1082586.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="PWU31_000007"
     CDS             complement(8479..8670)
                     /locus_tag="PWU31_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL1082587.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="PWU31_000008"
     CDS             complement(8691..9581)
                     /locus_tag="PWU31_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL1082588.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="PWU31_000009"
     CDS             complement(9568..10335)
                     /locus_tag="PWU31_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1082589.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PWU31_000010"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PWU31_000010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL1082590.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PWU31_000011"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PWU31_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL1082591.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PWU31_000012"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PWU31_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL1082592.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="PWU31_000013"
     CDS             14906..15289
                     /locus_tag="PWU31_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082593.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="PWU31_000014"
     CDS             complement(15457..16215)
                     /locus_tag="PWU31_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL1082594.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="PWU31_000015"
     CDS             complement(16233..17051)
                     /locus_tag="PWU31_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1082595.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="PWU31_000016"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="PWU31_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL1082596.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="PWU31_000017"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="PWU31_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL1082597.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="PWU31_000018"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="PWU31_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL1082598.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="PWU31_000019"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="PWU31_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL1082599.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="PWU31_000020"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="PWU31_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL1082600.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="PWU31_000021"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="PWU31_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL1082601.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="PWU31_000022"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="PWU31_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL1082602.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="PWU31_000023"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="PWU31_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL1082603.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="PWU31_000024"
     CDS             27093..27392
                     /locus_tag="PWU31_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL1082604.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="PWU31_000025"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="PWU31_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1082605.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /locus_tag="PWU31_000026"
     CDS             27980..28216
                     /locus_tag="PWU31_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL1082606.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="PWU31_000027"
     CDS             28359..30332
                     /locus_tag="PWU31_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL1082607.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="PWU31_000028"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="PWU31_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL1082608.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="PWU31_000029"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="PWU31_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL1082609.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="PWU31_000030"
     CDS             33211..34503
                     /locus_tag="PWU31_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL1082610.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35290..36153
                     /locus_tag="PWU31_000031"
     CDS             35290..36153
                     /locus_tag="PWU31_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1082611.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36282..37904)
                     /locus_tag="PWU31_000032"
     CDS             complement(36282..37904)
                     /locus_tag="PWU31_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082612.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38153..38674
                     /locus_tag="PWU31_000033"
     CDS             38153..38674
                     /locus_tag="PWU31_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL1082613.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="PWU31_000034"
     CDS             complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="PWU31_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL1082614.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40755..41573
                     /locus_tag="PWU31_000035"
     CDS             40755..41573
                     /locus_tag="PWU31_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL1082615.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41676..41873)
                     /locus_tag="PWU31_000036"
     CDS             complement(41676..41873)
                     /locus_tag="PWU31_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082616.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42042..42620
                     /locus_tag="PWU31_000037"
     CDS             42042..42620
                     /locus_tag="PWU31_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL1082617.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42715..42852
                     /locus_tag="PWU31_000038"
     CDS             42715..42852
                     /locus_tag="PWU31_000038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082618.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43665..44168)
                     /locus_tag="PWU31_000039"
     CDS             complement(43665..44168)
                     /locus_tag="PWU31_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1082619.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44527..45552
                     /locus_tag="PWU31_000040"
     CDS             44527..45552
                     /locus_tag="PWU31_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1082620.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45875..46753
                     /locus_tag="PWU31_000041"
     CDS             45875..46753
                     /locus_tag="PWU31_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL1082621.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47229..47345
                     /locus_tag="PWU31_000042"
     CDS             47229..47345
                     /locus_tag="PWU31_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL1082622.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48273..48611
                     /locus_tag="PWU31_000043"
     CDS             48273..48611
                     /locus_tag="PWU31_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082623.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48705..48935
                     /locus_tag="PWU31_000044"
     CDS             48705..48935
                     /locus_tag="PWU31_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082624.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49335..49706
                     /locus_tag="PWU31_000045"
     CDS             49335..49706
                     /locus_tag="PWU31_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL1082625.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49980..50672)
                     /locus_tag="PWU31_000046"
     CDS             complement(49980..50672)
                     /locus_tag="PWU31_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1082626.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50675..51460)
                     /locus_tag="PWU31_000047"
     CDS             complement(50675..51460)
                     /locus_tag="PWU31_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1082627.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51697..53097
                     /gene="lacG"
                     /locus_tag="PWU31_000048"
     CDS             51697..53097
                     /gene="lacG"
                     /locus_tag="PWU31_000048"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL1082628.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53122..53439
                     /locus_tag="PWU31_000049"
     CDS             53122..53439
                     /locus_tag="PWU31_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1082629.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53452..55125
                     /locus_tag="PWU31_000050"
     CDS             53452..55125
                     /locus_tag="PWU31_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL1082630.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55139..56770
                     /locus_tag="PWU31_000051"
     CDS             55139..56770
                     /locus_tag="PWU31_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL1082631.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57261..58826)
                     /gene="guaA"
                     /locus_tag="PWU31_000052"
     CDS             complement(57261..58826)
                     /gene="guaA"
                     /locus_tag="PWU31_000052"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1082632.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58885..59808)
                     /locus_tag="PWU31_000053"
     CDS             complement(58885..59808)
                     /locus_tag="PWU31_000053"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL1082633.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            60004..60609
                     /locus_tag="PWU31_000054"
     CDS             60004..60609
                     /locus_tag="PWU31_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1082634.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60917..61933
                     /locus_tag="PWU31_000055"
     CDS             60917..61933
                     /locus_tag="PWU31_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1082635.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62074..63375
                     /locus_tag="PWU31_000056"
     CDS             62074..63375
                     /locus_tag="PWU31_000056"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL1082636.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63421..64083
                     /gene="deoC"
                     /locus_tag="PWU31_000057"
     CDS             63421..64083
                     /gene="deoC"
                     /locus_tag="PWU31_000057"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL1082637.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64104..64502
                     /locus_tag="PWU31_000058"
     CDS             64104..64502
                     /locus_tag="PWU31_000058"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL1082638.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64568..65650
                     /locus_tag="PWU31_000059"
     CDS             64568..65650
                     /locus_tag="PWU31_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1082639.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65862..67424
                     /locus_tag="PWU31_000060"
     CDS             65862..67424
                     /locus_tag="PWU31_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082640.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67417..68550
                     /locus_tag="PWU31_000061"
     CDS             67417..68550
                     /locus_tag="PWU31_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082641.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68550..69506
                     /locus_tag="PWU31_000062"
     CDS             68550..69506
                     /locus_tag="PWU31_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082642.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69605..70771
                     /gene="deoB"
                     /locus_tag="PWU31_000063"
     CDS             69605..70771
                     /gene="deoB"
                     /locus_tag="PWU31_000063"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL1082643.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70795..71613
                     /locus_tag="PWU31_000064"
     CDS             70795..71613
                     /locus_tag="PWU31_000064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1082644.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71630..72337
                     /gene="deoD"
                     /locus_tag="PWU31_000065"
     CDS             71630..72337
                     /gene="deoD"
                     /locus_tag="PWU31_000065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1082645.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72635..73321)
                     /gene="gpmA"
                     /locus_tag="PWU31_000066"
     CDS             complement(72635..73321)
                     /gene="gpmA"
                     /locus_tag="PWU31_000066"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1082646.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73493..74074)
                     /locus_tag="PWU31_000067"
     CDS             complement(73493..74074)
                     /locus_tag="PWU31_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1082647.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74207..74890)
                     /gene="rpiA"
                     /locus_tag="PWU31_000068"
     CDS             complement(74207..74890)
                     /gene="rpiA"
                     /locus_tag="PWU31_000068"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL1082648.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75209..75622
                     /gene="rpsL"
                     /locus_tag="PWU31_000069"
     CDS             75209..75622
                     /gene="rpsL"
                     /locus_tag="PWU31_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL1082649.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75706..76176
                     /gene="rpsG"
                     /locus_tag="PWU31_000070"
     CDS             75706..76176
                     /gene="rpsG"
                     /locus_tag="PWU31_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL1082650.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76270..78357
                     /gene="fusA"
                     /locus_tag="PWU31_000071"
     CDS             76270..78357
                     /gene="fusA"
                     /locus_tag="PWU31_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL1082651.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78529..79716
                     /gene="tuf"
                     /locus_tag="PWU31_000072"
     CDS             78529..79716
                     /gene="tuf"
                     /locus_tag="PWU31_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1082652.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80123..80431
                     /gene="rpsJ"
                     /locus_tag="PWU31_000073"
     CDS             80123..80431
                     /gene="rpsJ"
                     /locus_tag="PWU31_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL1082653.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80462..81091
                     /gene="rplC"
                     /locus_tag="PWU31_000074"
     CDS             80462..81091
                     /gene="rplC"
                     /locus_tag="PWU31_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL1082654.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81119..81742
                     /gene="rplD"
                     /locus_tag="PWU31_000075"
     CDS             81119..81742
                     /gene="rplD"
                     /locus_tag="PWU31_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL1082655.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81742..82032
                     /gene="rplW"
                     /locus_tag="PWU31_000076"
     CDS             81742..82032
                     /gene="rplW"
                     /locus_tag="PWU31_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL1082656.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82071..82904
                     /gene="rplB"
                     /locus_tag="PWU31_000077"
     CDS             82071..82904
                     /gene="rplB"
                     /locus_tag="PWU31_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL1082657.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82947..83225
                     /gene="rpsS"
                     /locus_tag="PWU31_000078"
     CDS             82947..83225
                     /gene="rpsS"
                     /locus_tag="PWU31_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL1082658.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83248..83595
                     /gene="rplV"
                     /locus_tag="PWU31_000079"
     CDS             83248..83595
                     /gene="rplV"
                     /locus_tag="PWU31_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL1082659.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83609..84265
                     /gene="rpsC"
                     /locus_tag="PWU31_000080"
     CDS             83609..84265
                     /gene="rpsC"
                     /locus_tag="PWU31_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL1082660.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84268..84702
                     /gene="rplP"
                     /locus_tag="PWU31_000081"
     CDS             84268..84702
                     /gene="rplP"
                     /locus_tag="PWU31_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL1082661.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84692..84880
                     /gene="rpmC"
                     /locus_tag="PWU31_000082"
     CDS             84692..84880
                     /gene="rpmC"
                     /locus_tag="PWU31_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL1082662.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84905..85171
                     /gene="rpsQ"
                     /locus_tag="PWU31_000083"
     CDS             84905..85171
                     /gene="rpsQ"
                     /locus_tag="PWU31_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL1082663.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85229..85597
                     /gene="rplN"
                     /locus_tag="PWU31_000084"
     CDS             85229..85597
                     /gene="rplN"
                     /locus_tag="PWU31_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL1082664.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85634..85942
                     /gene="rplX"
                     /locus_tag="PWU31_000085"
     CDS             85634..85942
                     /gene="rplX"
                     /locus_tag="PWU31_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL1082665.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85970..86509
                     /gene="rplE"
                     /locus_tag="PWU31_000086"
     CDS             85970..86509
                     /gene="rplE"
                     /locus_tag="PWU31_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL1082666.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86528..86713
                     /locus_tag="PWU31_000087"
     CDS             86528..86713
                     /locus_tag="PWU31_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL1082667.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86749..87147
                     /gene="rpsH"
                     /locus_tag="PWU31_000088"
     CDS             86749..87147
                     /gene="rpsH"
                     /locus_tag="PWU31_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL1082668.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87179..87715
                     /gene="rplF"
                     /locus_tag="PWU31_000089"
     CDS             87179..87715
                     /gene="rplF"
                     /locus_tag="PWU31_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL1082669.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87876..88232
                     /gene="rplR"
                     /locus_tag="PWU31_000090"
     CDS             87876..88232
                     /gene="rplR"
                     /locus_tag="PWU31_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL1082670.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88253..88753
                     /gene="rpsE"
                     /locus_tag="PWU31_000091"
     CDS             88253..88753
                     /gene="rpsE"
                     /locus_tag="PWU31_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL1082671.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88768..88947
                     /gene="rpmD"
                     /locus_tag="PWU31_000092"
     CDS             88768..88947
                     /gene="rpmD"
                     /locus_tag="PWU31_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL1082672.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88991..89431
                     /gene="rplO"
                     /locus_tag="PWU31_000093"
     CDS             88991..89431
                     /gene="rplO"
                     /locus_tag="PWU31_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL1082673.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89431..90726
                     /gene="secY"
                     /locus_tag="PWU31_000094"
     CDS             89431..90726
                     /gene="secY"
                     /locus_tag="PWU31_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL1082674.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90788..91435
                     /locus_tag="PWU31_000095"
     CDS             90788..91435
                     /locus_tag="PWU31_000095"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL1082675.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91626..91844
                     /gene="infA"
                     /locus_tag="PWU31_000096"
     CDS             91626..91844
                     /gene="infA"
                     /locus_tag="PWU31_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL1082676.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91877..91993
                     /gene="rpmJ"
                     /locus_tag="PWU31_000097"
     CDS             91877..91993
                     /gene="rpmJ"
                     /locus_tag="PWU31_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL1082677.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92011..92376
                     /gene="rpsM"
                     /locus_tag="PWU31_000098"
     CDS             92011..92376
                     /gene="rpsM"
                     /locus_tag="PWU31_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL1082678.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92404..92793
                     /gene="rpsK"
                     /locus_tag="PWU31_000099"
     CDS             92404..92793
                     /gene="rpsK"
                     /locus_tag="PWU31_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL1082679.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92873..93811
                     /locus_tag="PWU31_000100"
     CDS             92873..93811
                     /locus_tag="PWU31_000100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL1082680.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93839..94219
                     /gene="rplQ"
                     /locus_tag="PWU31_000101"
     CDS             93839..94219
                     /gene="rplQ"
                     /locus_tag="PWU31_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL1082681.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94491..95330
                     /locus_tag="PWU31_000102"
     CDS             94491..95330
                     /locus_tag="PWU31_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1082682.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95306..96175
                     /locus_tag="PWU31_000103"
     CDS             95306..96175
                     /locus_tag="PWU31_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1082683.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96165..96962
                     /locus_tag="PWU31_000104"
     CDS             96165..96962
                     /locus_tag="PWU31_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL1082684.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97165..97911
                     /gene="truA"
                     /locus_tag="PWU31_000105"
     CDS             97165..97911
                     /gene="truA"
                     /locus_tag="PWU31_000105"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1082685.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(98366..101035)
                     /locus_tag="PWU31_000106"
     CDS             complement(98366..101035)
                     /locus_tag="PWU31_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL1082686.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(101273..101461)
                     /locus_tag="PWU31_000107"
     CDS             complement(101273..101461)
                     /locus_tag="PWU31_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL1082687.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            101731..102093
                     /locus_tag="PWU31_000108"
     CDS             101731..102093
                     /locus_tag="PWU31_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1082688.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            102145..103827
                     /locus_tag="PWU31_000109"
     CDS             102145..103827
                     /locus_tag="PWU31_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL1082689.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            103944..105980
                     /gene="recG"
                     /locus_tag="PWU31_000110"
     CDS             103944..105980
                     /gene="recG"
                     /locus_tag="PWU31_000110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL1082690.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            106029..107030
                     /gene="plsX"
                     /locus_tag="PWU31_000111"
     CDS             106029..107030
                     /gene="plsX"
                     /locus_tag="PWU31_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL1082691.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            107152..107394
                     /gene="acpP"
                     /locus_tag="PWU31_000112"
     CDS             107152..107394
                     /gene="acpP"
                     /locus_tag="PWU31_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1082692.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            107662..108666
                     /locus_tag="PWU31_000113"
     CDS             107662..108666
                     /locus_tag="PWU31_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082693.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            108667..109611
                     /locus_tag="PWU31_000114"
     CDS             108667..109611
                     /locus_tag="PWU31_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082694.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            109611..110573
                     /locus_tag="PWU31_000115"
     CDS             109611..110573
                     /locus_tag="PWU31_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082695.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            110600..111514
                     /locus_tag="PWU31_000116"
     CDS             110600..111514
                     /locus_tag="PWU31_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082696.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            111540..113321
                     /locus_tag="PWU31_000117"
     CDS             111540..113321
                     /locus_tag="PWU31_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1082697.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            113669..114355
                     /locus_tag="PWU31_000118"
     CDS             113669..114355
                     /locus_tag="PWU31_000118"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL1082698.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            114375..117956
                     /gene="smc"
                     /locus_tag="PWU31_000119"
     CDS             114375..117956
                     /gene="smc"
                     /locus_tag="PWU31_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL1082699.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            117965..118774
                     /locus_tag="PWU31_000120"
     CDS             117965..118774
                     /locus_tag="PWU31_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1082700.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            118785..119783
                     /gene="ftsY"
                     /locus_tag="PWU31_000121"
     CDS             118785..119783
                     /gene="ftsY"
                     /locus_tag="PWU31_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL1082701.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            119970..122237
                     /gene="gshAB"
                     /locus_tag="PWU31_000122"
     CDS             119970..122237
                     /gene="gshAB"
                     /locus_tag="PWU31_000122"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL1082702.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(122326..122778)
                     /locus_tag="PWU31_000123"
     CDS             complement(122326..122778)
                     /locus_tag="PWU31_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL1082703.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            122928..123878
                     /locus_tag="PWU31_000124"
     CDS             122928..123878
                     /locus_tag="PWU31_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1082704.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            123871..124830
                     /locus_tag="PWU31_000125"
     CDS             123871..124830
                     /locus_tag="PWU31_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1082705.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            124827..125582
                     /locus_tag="PWU31_000126"
     CDS             124827..125582
                     /locus_tag="PWU31_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082706.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            125605..126558
                     /locus_tag="PWU31_000127"
     CDS             125605..126558
                     /locus_tag="PWU31_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1082707.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(127202..127498)
                     /locus_tag="PWU31_000128"
     CDS             complement(127202..127498)
                     /locus_tag="PWU31_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL1082708.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            127853..128209
                     /locus_tag="PWU31_000129"
     CDS             127853..128209
                     /locus_tag="PWU31_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1082709.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            128219..129118
                     /locus_tag="PWU31_000130"
     CDS             128219..129118
                     /locus_tag="PWU31_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL1082710.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            129634..130365
                     /locus_tag="PWU31_000131"
     CDS             129634..130365
                     /locus_tag="PWU31_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1082711.1"
                     /translation="MMKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            130510..132537
                     /locus_tag="PWU31_000132"
     CDS             130510..132537
                     /locus_tag="PWU31_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL1082712.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            132645..133742
                     /locus_tag="PWU31_000133"
     CDS             132645..133742
                     /locus_tag="PWU31_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL1082713.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            134049..>134496
                     /locus_tag="PWU31_000134"
     CDS             134049..>134496
                     /locus_tag="PWU31_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082714.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        42826 a        24467 c        28061 g        39142 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agacattgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga
    25441 aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa
    25501 agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc
    26881 tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    34621 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    34681 tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag
    34741 atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct
    34801 ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    34861 tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg gagctaaacg
    34921 gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
    34981 aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact caggtcaaaa
    35041 cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg gaaataagcc
    35101 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    35161 accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt
    35221 gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg
    35281 ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta
    35341 gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta
    35401 gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg
    35461 atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg
    35521 gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt
    35581 agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt
    35641 tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg
    35701 aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa
    35761 gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc
    35821 aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa
    35881 gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc
    35941 gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac
    36001 gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg
    36061 aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt
    36121 atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc
    36181 ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct
    36241 tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc
    36301 aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg
    36361 atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg
    36421 tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg
    36481 attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa
    36541 agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt
    36601 ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta
    36661 tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg
    36721 aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact
    36781 gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata
    36841 tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct
    36901 aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca
    36961 atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg
    37021 tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca
    37081 ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca
    37141 tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt
    37201 cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc
    37261 aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct
    37321 tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca
    37381 tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat
    37441 tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct
    37501 gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct
    37561 cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc
    37621 acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag
    37681 tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct
    37741 gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt
    37801 gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt
    37861 ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt
    37921 tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa
    37981 ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc
    38041 ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat
    38101 ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga
    38161 aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt
    38221 gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt
    38281 tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg
    38341 atataaagga aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt
    38401 cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat
    38461 tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata
    38521 ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt
    38581 taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt
    38641 tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg
    38701 gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata
    38761 tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata
    38821 cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa
    38881 ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc
    38941 ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt
    39001 gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg
    39061 aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc
    39121 gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc
    39181 ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt
    39241 gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa
    39301 tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat
    39361 agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat
    39421 agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg
    39481 attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga
    39541 tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc
    39601 agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat
    39661 cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa
    39721 ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat
    39781 cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc
    39841 aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg
    39901 aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc
    39961 aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt
    40021 ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc
    40081 aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata
    40141 ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg
    40201 attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata
    40261 gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg
    40321 tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc
    40381 gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg
    40441 acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt
    40501 gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac
    40561 cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac
    40621 ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt
    40681 ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa
    40741 gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg
    40801 ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta
    40861 aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg
    40921 ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata
    40981 ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag
    41041 ataaaacact gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc
    41101 atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt
    41161 tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat
    41221 tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg
    41281 tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag
    41341 cttctggaat cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg
    41401 agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg
    41461 aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg
    41521 gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa
    41581 aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt
    41641 aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta
    41701 tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc
    41761 gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca
    41821 attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac
    41881 cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct
    41941 ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct
    42001 ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg
    42061 aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg
    42121 aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata
    42181 ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg
    42241 tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc
    42301 tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga
    42361 cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg
    42421 tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat
    42481 cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag
    42541 tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca
    42601 tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa
    42661 aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat
    42721 caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat
    42781 caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca
    42841 ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt
    42901 acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa
    42961 agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta agagataaaa
    43021 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
    43081 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
    43141 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
    43201 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
    43261 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
    43321 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
    43381 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
    43441 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
    43501 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
    43561 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
    43621 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
    43681 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
    43741 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
    43801 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
    43861 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
    43921 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
    43981 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
    44041 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
    44101 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
    44161 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
    44221 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
    44281 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
    44341 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
    44401 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
    44461 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
    44521 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
    44581 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
    44641 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
    44701 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
    44761 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
    44821 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
    44881 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
    44941 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
    45001 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
    45061 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
    45121 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
    45181 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
    45241 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
    45301 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
    45361 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
    45421 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
    45481 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
    45541 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
    45601 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
    45661 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
    45721 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
    45781 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
    45841 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
    45901 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
    45961 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
    46021 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
    46081 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
    46141 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
    46201 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
    46261 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
    46321 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
    46381 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
    46441 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
    46501 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
    46561 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
    46621 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
    46681 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
    46741 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
    46801 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
    46861 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttgtttc
    46921 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
    46981 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
    47041 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
    47101 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
    47161 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
    47221 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
    47281 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
    47341 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
    47401 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
    47461 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
    47521 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
    47581 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
    47641 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
    47701 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
    47761 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
    47821 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
    47881 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
    47941 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
    48001 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
    48061 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
    48121 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
    48181 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
    48241 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
    48301 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
    48361 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
    48421 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
    48481 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
    48541 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
    48601 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
    48661 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
    48721 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
    48781 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
    48841 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
    48901 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
    48961 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
    49021 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
    49081 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
    49141 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
    49201 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
    49261 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
    49321 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
    49381 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
    49441 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
    49501 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
    49561 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
    49621 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
    49681 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
    49741 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
    49801 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
    49861 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
    49921 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
    49981 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
    50041 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
    50101 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
    50161 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
    50221 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
    50281 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
    50341 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
    50401 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
    50461 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
    50521 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
    50581 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
    50641 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
    50701 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
    50761 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
    50821 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
    50881 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
    50941 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
    51001 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
    51061 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
    51121 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
    51181 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
    51241 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
    51301 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
    51361 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
    51421 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
    51481 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
    51541 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
    51601 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
    51661 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
    51721 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
    51781 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
    51841 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
    51901 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
    51961 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
    52021 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
    52081 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
    52141 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
    52201 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
    52261 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
    52321 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
    52381 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
    52441 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
    52501 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
    52561 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
    52621 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
    52681 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
    52741 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
    52801 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
    52861 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
    52921 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
    52981 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
    53041 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
    53101 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
    53161 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
    53221 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
    53281 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
    53341 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
    53401 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    53461 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    53521 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    53581 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    53641 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    53701 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    53761 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    53821 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    53881 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    53941 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    54001 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    54061 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    54121 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    54181 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    54241 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    54301 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    54361 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    54421 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    54481 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    54541 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    54601 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    54661 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    54721 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    54781 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    54841 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    54901 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    54961 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    55021 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    55081 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    55141 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    55201 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    55261 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    55321 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    55381 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    55441 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    55501 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    55561 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    55621 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    55681 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    55741 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    55801 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    55861 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    55921 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    55981 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    56041 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    56101 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    56161 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    56221 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    56281 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    56341 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    56401 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    56461 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    56521 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    56581 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    56641 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    56701 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    56761 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    56821 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    56881 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    56941 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    57001 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    57061 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    57121 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    57181 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc
    57241 ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt agaagtgatg
    57301 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
    57361 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
    57421 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
    57481 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
    57541 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
    57601 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
    57661 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
    57721 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
    57781 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
    57841 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
    57901 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
    57961 cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
    58021 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
    58081 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
    58141 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
    58201 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
    58261 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
    58321 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
    58381 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
    58441 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
    58501 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
    58561 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
    58621 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
    58681 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
    58741 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
    58801 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
    58861 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
    58921 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
    58981 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
    59041 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
    59101 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
    59161 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
    59221 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
    59281 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
    59341 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
    59401 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
    59461 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
    59521 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
    59581 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
    59641 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
    59701 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
    59761 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
    59821 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
    59881 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
    59941 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
    60001 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
    60061 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
    60121 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
    60181 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
    60241 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
    60301 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
    60361 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
    60421 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
    60481 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
    60541 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
    60601 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
    60661 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
    60721 ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca
    60781 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
    60841 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
    60901 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
    60961 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
    61021 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
    61081 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
    61141 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
    61201 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
    61261 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
    61321 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
    61381 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
    61441 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
    61501 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
    61561 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
    61621 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
    61681 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
    61741 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
    61801 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
    61861 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
    61921 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
    61981 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
    62041 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
    62101 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
    62161 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
    62221 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
    62281 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
    62341 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
    62401 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
    62461 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
    62521 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
    62581 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
    62641 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
    62701 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
    62761 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
    62821 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
    62881 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
    62941 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
    63001 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
    63061 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
    63121 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
    63181 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
    63241 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
    63301 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
    63361 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
    63421 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
    63481 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
    63541 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
    63601 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
    63661 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
    63721 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
    63781 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
    63841 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
    63901 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
    63961 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
    64021 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
    64081 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
    64141 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
    64201 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
    64261 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
    64321 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
    64381 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
    64441 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
    64501 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
    64561 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
    64621 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
    64681 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
    64741 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
    64801 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
    64861 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
    64921 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
    64981 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
    65041 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
    65101 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
    65161 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
    65221 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
    65281 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
    65341 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
    65401 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
    65461 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
    65521 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
    65581 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
    65641 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
    65701 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
    65761 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
    65821 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
    65881 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
    65941 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
    66001 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
    66061 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
    66121 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
    66181 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
    66241 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
    66301 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
    66361 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
    66421 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
    66481 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
    66541 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
    66601 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
    66661 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
    66721 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
    66781 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
    66841 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
    66901 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
    66961 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
    67021 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
    67081 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
    67141 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
    67201 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    67261 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    67321 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    67381 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    67441 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    67501 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    67561 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    67621 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    67681 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    67741 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    67801 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    67861 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    67921 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    67981 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    68041 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    68101 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    68161 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    68221 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    68281 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    68341 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    68401 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    68461 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    68521 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    68581 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    68641 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    68701 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    68761 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    68821 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    68881 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    68941 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    69001 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    69061 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    69121 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    69181 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    69241 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    69301 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    69361 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    69421 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    69481 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    69541 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    69601 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    69661 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    69721 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    69781 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    69841 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    69901 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    69961 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    70021 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    70081 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    70141 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    70201 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    70261 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    70321 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    70381 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    70441 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    70501 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    70561 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    70621 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    70681 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    70741 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    70801 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    70861 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    70921 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    70981 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    71041 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    71101 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    71161 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    71221 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    71281 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    71341 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    71401 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    71461 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    71521 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    71581 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    71641 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    71701 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    71761 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    71821 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    71881 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    71941 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    72001 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    72061 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    72121 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    72181 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    72241 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    72301 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    72361 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    72421 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    72481 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    72541 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    72601 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    72661 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    72721 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    72781 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    72841 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    72901 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    72961 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    73021 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    73081 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    73141 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    73201 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    73261 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    73321 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    73381 tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag
    73441 tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg tattagatgc
    73501 ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt ccaactgaaa
    73561 caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag agacgtcgga
    73621 tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct tttttacgag
    73681 cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg tttgctaggt
    73741 tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct tcatcaagac
    73801 taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat tgccgcgtag
    73861 atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc atgtcagcga
    73921 tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat gtctcatgga
    73981 aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa tcaacgtcat
    74041 ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag cagtgatttt
    74101 ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata gttttttcgc
    74161 aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat ttacgacgga
    74221 ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa accatgttta
    74281 ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct aagctttctg
    74341 gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct gtggttaaga
    74401 cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca aaaatatgaa
    74461 gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc ccaagtttat
    74521 cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta gcgactattt
    74581 tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca ctgatttcat
    74641 cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt aacggtattc
    74701 ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac
    74761 cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt gaaccggtcc
    74821 cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca gctaattgtt
    74881 ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc ctcactattt
    74941 tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc tttttcaaaa
    75001 aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa aggtgcttta
    75061 tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt gataaggatg
    75121 cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt ggaactagac
    75181 gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag tacgtaaacc
    75241 tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat ataacagctt
    75301 taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg
    75361 tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc
    75421 aaacttaatt gaagttactg cttatatccc agggattggt cataacttac aagaacacag
    75481 tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt accatatcgt
    75541 tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg
    75601 aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc ttctactata
    75661 tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc acgtaaaggt
    75721 cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt agtaactcgc
    75781 ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat tatctataat
    75841 gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt tgaacaagca
    75901 atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac
    75961 caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg ttgggtagta
    76021 aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa agaaatcatg
    76081 gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca taaaatggct
    76141 gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt ggtcggcttt
    76201 tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa gagaggagaa
    76261 acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg tatcatggcc
    76321 cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac tggtaaaatc
    76381 cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga acaagaacaa
    76441 gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg tcaccgaatc
    76501 aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg ttcattgcgt
    76561 gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa
    76621 acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc aaacaaaatg
    76681 gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg tttgcaagca
    76741 aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg aattatcgac
    76801 cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat cgaagaaaca
    76861 gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt ggttgaagct
    76921 gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga aatcactgaa
    76981 gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta cccagtacta
    77041 tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt tcttgattac
    77101 ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac tgatgaagaa
    77161 gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa agttatgact
    77221 gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt acaatcaggt
    77281 tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg
    77341 catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc tgctgctgtt
    77401 ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct agttattctt
    77461 gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa atcaaaagct
    77521 gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc tactttccgt
    77581 gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga acttcactta
    77641 gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct
    77701 caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt
    77761 cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc aaatgaagaa
    77821 ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt
    77881 ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc tggctatcca
    77941 ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga ttcaaatgaa
    78001 actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa agctaatcca
    78061 gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta tttaggtgat
    78121 gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc
    78181 ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta tgctacaact
    78241 cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca ctacgaagat
    78301 gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc tgaataattt
    78361 cttgaaaccg gatagtcctg caaagagatt atccaaagag actatatttt taatatagtt
    78421 tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac atcatatacc
    78481 tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat ggcaaaagaa
    78541 aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt tgaccatggt
    78601 aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg cggccaagct
    78661 atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat cacaatctca
    78721 acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga ctgcccagga
    78781 cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcttg
    78841 gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct attgtctcgt
    78901 caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt tgatgacgaa
    78961 gaattactag aattagttga aatggaagtt cgtgacctat taacagaata cgaattccct
    79021 ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg cgacgcttca
    79081 tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc aactccagaa
    79141 cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat tactggacgt
    79201 ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa
    79261 gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga aatgttccgt
    79321 aaattgttag actacgctga agctggagac aacattggtg ctttactacg tggtgttgca
    79381 cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac acctcataca
    79441 aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca tactccattc
    79501 ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg tgttgttgaa
    79561 ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga agttgaatta
    79621 atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg cggacgtaca
    79681 gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt aaagtaaagc
    79741 aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt tccgagcttt
    79801 tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag aaacacgaaa
    79861 taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa
    79921 aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta ctttagtgaa
    79981 ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc
    80041 ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc tacttttcaa
    80101 aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta
    80161 tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg
    80221 agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca caatcatccg
    80281 tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac acaaacgtct
    80341 tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc tagacttacc
    80401 atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg gaggtgtact
    80461 catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg
    80521 tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac aagtgaaaac
    80581 aatggaaacg gatggctacg aagctattca agttggttac caagatatgc gtgaagtttt
    80641 atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta agcgcttcat
    80701 tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa ttacagtaga
    80761 tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta aaggattcca
    80821 aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt ctcgttacca
    80881 tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa acaaacgtct
    80941 tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg tacgtgtcga
    81001 tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa aatctttaat
    81061 tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga actaaggaat
    81121 gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca cacttaacga
    81181 agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg
    81241 tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg
    81301 acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta tccgctcacc
    81361 acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca gctacaaact
    81421 tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa
    81481 caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag aattcaaaca
    81541 agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa aaggtaacga
    81601 ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt ctgataatgt
    81661 gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg ctcttactca
    81721 aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc agtgatcact
    81781 gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga cactcgcgca
    81841 aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt caaaaacgtg
    81901 aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg atatacaaaa
    81961 aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca attattcgaa
    82021 gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac gtggcgatta
    82081 aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat tttgctgaaa
    82141 tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat gctggacgta
    82201 acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg
    82261 tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg atcgaatatg
    82321 atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg aaagcttaca
    82381 tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca gaagcagata
    82441 tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt atccacaaca
    82501 ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca agtgctcaag
    82561 tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa gttcgtatga
    82621 tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac gaattaatca
    82681 acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg
    82741 taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct atcggacgta
    82801 aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag
    82861 ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg
    82921 tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg
    82981 atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa aaagttatca
    83041 aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc atcgcagttt
    83101 atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga cacaaattag
    83161 gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag aaaacgaaac
    83221 gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc taaagcaact
    83281 gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct tatcagaggt
    83341 aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc tgctggaatc
    83401 attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga cttagatgtt
    83461 gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa acgtttccgt
    83521 cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat cacggtagtc
    83581 gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg
    83641 tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt atgctgagtt
    83701 cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg atgctgctgt
    83761 atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc acacagctaa
    83821 accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa
    83881 caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac cagatctaga
    83941 tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg
    84001 cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga tcaaaactca
    84061 agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact cagaaggaac
    84121 tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag cagatacaac
    84181 atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc caactaaaaa
    84241 aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt
    84301 gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc attcggtgaa
    84361 tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga agctgctcgt
    84421 atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat tttccctcac
    84481 aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg ggcacctgaa
    84541 ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa
    84601 gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa aactaagatc
    84661 gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca gagaattaac
    84721 cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta atcttagatt
    84781 ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat
    84841 tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg aggccattaa
    84901 gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa
    84961 aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta tctatggcaa
    85021 acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag caaaagttgg
    85081 cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt tccgtttgtt
    85141 ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat atagattgaa
    85201 tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc
    85261 tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat ctggtcgtaa
    85321 aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc caggtggggt
    85381 tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg
    85441 tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc gtgatgataa
    85501 aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg aaaacaactt
    85561 catgaagatc gtttcactag caccagaagt tttataatct tgagacactt atcaaaggag
    85621 gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga
    85681 caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag ttattgtcga
    85741 aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc aaggtggaat
    85801 tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact ctactggtgt
    85861 agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac
    85921 cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct
    85981 aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta actatagctc
    86041 tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt
    86101 atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca ctggtcaaaa
    86161 accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc
    86221 aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag acaaattggt
    86281 ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag cctttgacgg
    86341 tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag tagattatga
    86401 tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa actctgatga
    86461 agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat taaaggaggc
    86521 gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa aattttcaac
    86581 acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc gtaagttcca
    86641 tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa
    86701 agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat ggtcatgaca
    86761 gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa acatgagtct
    86821 ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt
    86881 ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg tgttttccta
    86941 aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc taaaccaggt
    87001 ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt aggtatcgca
    87061 atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc
    87121 ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt gtctttaagt
    87181 gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta ctcaagacgg
    87241 aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt cagcagatat
    87301 caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat
    87361 gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga
    87421 aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat tacaaggatc
    87481 aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac cagctggagt
    87541 aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca aagaagaagt
    87601 tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg
    87661 tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta aataatcgtt
    87721 tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag tttaccttca
    87781 aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt tactgcttga
    87841 aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca gataagaaca
    87901 aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc
    87961 cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt gatgacgtag
    88021 cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt ggaacaaaaa
    88081 cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta
    88141 aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg
    88201 aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat tcatggttta
    88261 tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa
    88321 agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa
    88381 cggacacgta ggatttggta caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc
    88441 aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa caatcccaca
    88501 cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg tagaaggttc
    88561 tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg tagcagatat
    88621 cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg
    88681 tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat cagttgaaga
    88741 attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt aaaacgcagt
    88801 attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct tacaaagatt
    88861 aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa tactgtttct
    88921 cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac attaaggagg
    88981 tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa
    89041 ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg gacaaaaagg
    89101 acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac aaacaccatt
    89161 gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact atgcagtcat
    89221 taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg ttgctttagt
    89281 tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta atggggaact
    89341 aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag aagcaattga
    89401 agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga tgcttttaaa
    89461 gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt atttcgccta
    89521 ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg
    89581 ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt ttccattttc
    89641 tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct tcaaatggac
    89701 atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat
    89761 caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg tatcactgct
    89821 ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc aacttacgta
    89881 acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg agaacagatc
    89941 acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat catttctaga
    90001 ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga tccttcagac
    90061 atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt gatcgtgaca
    90121 ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac gaaacgcgta
    90181 gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg agttattcca
    90241 gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc attgagcggt
    90301 agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta taatacagca
    90361 cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta cgcctttgtt
    90421 caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta tataccaagc
    90481 gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg tttaagtaca
    90541 gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca aatggtttgg
    90601 gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat cggtgtagct
    90661 ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt tggattcatt
    90721 gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt tattaggagg
    90781 gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa cacaagctga
    90841 aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt tccgcgcagc
    90901 gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata aaggtgcatt
    90961 agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac cagatacaga
    91021 gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag ctctggacgc
    91081 aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg ttggtgaaga
    91141 aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag caacttacca
    91201 taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt
    91261 ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta tcaacatcaa
    91321 aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca ctattgatgg
    91381 tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa aatagcacga
    91441 ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa attatgatag
    91501 aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga attttgaggt
    91561 gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac
    91621 tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa actttgccga
    91681 atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact gtttcaggta
    91741 aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt gaattgtctc
    91801 catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac tccgtaaaac
    91861 tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa
    91921 agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa aacataaaca
    91981 acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt agatattcct
    92041 cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa cactactgct
    92101 aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga attaacgaat
    92161 gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg ggatcttcgt
    92221 cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg tggaatccgt
    92281 catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa
    92341 ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg aggttaaaac
    92401 ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt
    92461 attgcacata tccattctac attcaataac actatcatca tgatcactga tactcacgga
    92521 aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa aaaatcaact
    92581 ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga acacggatta
    92641 aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc aattcgttca
    92701 ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt tcctcataat
    92761 ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc
    92821 agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa gaatgattga
    92881 gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca agttcatcgt
    92941 tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc gtattttatt
    93001 atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac tgcatgaatt
    93061 ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca ttaaaggctt
    93121 agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta ccggacctgc
    93181 tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta acaaagatat
    93241 gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag tgaaacctgg
    93301 acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg gcgttcttcc
    93361 agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga acacacgtgt
    93421 tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg gttcaattga
    93481 accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg atatttttgt
    93541 gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa
    93601 agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct cttacaattg
    93661 tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg agcctgaaat
    93721 gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa aattgcacga
    93781 cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg aaaccaacgt
    93841 gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac
    93901 aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga aagaagttcg
    93961 ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg cacgtcgtca
    94021 agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt
    94081 cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg cagaacgtca
    94141 aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg caccaatggt
    94201 tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga ttcttttaga
    94261 gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc tcaagcgaat
    94321 atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa ttacctgtca
    94381 tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt gaaaattttg
    94441 tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc atggagccaa
    94501 ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc cccgcattga
    94561 aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga cacaatggtt
    94621 caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa tcagggatcg
    94681 tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga cagatggttg
    94741 gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa gatgacgttg
    94801 cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt gtgaaagatg
    94861 cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt ttatcaggag
    94921 ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat atcattattt
    94981 tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc tctacgatca
    95041 agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat attgatgaag
    95101 cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt gaaggtacac
    95161 caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat ctaccatttc
    95221 ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt gaatatatga
    95281 cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag acttcactta
    95341 tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga cgatcaaaga
    95401 aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct tacttcagca
    95461 tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac gagatattca
    95521 gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca tcgtctttca
    95581 gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa
    95641 aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat tagaacaagt
    95701 tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg
    95761 tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat tggatgaacc
    95821 aacggcagga cttgatcccc agggcagaaa agaaatgatg gagatgtttt ggcgtcttca
    95881 taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg tagccaattt
    95941 tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga
    96001 ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga
    96061 gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt tgacagctga
    96121 agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg aataaattga
    96181 tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc cgagctaaat
    96241 tattagccag tttttatttt atcggaatca tttttttagc aaataattgg gaaacgtacc
    96301 ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat ttgaaatttt
    96361 ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct ttgcaggtct
    96421 tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc actgaatttg
    96481 gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt atgtcgactc
    96541 tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac ttgcttcgtc
    96601 cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg attgctcttc
    96661 gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg
    96721 gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt cctttgctga
    96781 ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct atggaagcaa
    96841 gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg cataatagag
    96901 acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt ttaagaggat
    96961 aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc
    97021 aagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca ggcctctttc
    97081 ttttatctga aaagaaagaa cgaaaaaatt ttatagaaaa atcattattc cagataacct
    97141 tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc gtacgacgga
    97201 acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga agaagtagaa
    97261 aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc gggaaggaca
    97321 gatgccggag tacatgcgat cgggcaagta atccattttg attatccaga ggagcgtccg
    97381 ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc tgtaagacaa
    97441 gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa gacgtatcaa
    97501 tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc aagttatttt
    97561 ccttacccac ttgatttgga aaaaattcag cgggcgcttc ctgatttatt agggactcac
    97621 gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaaatacg tacgatctac
    97681 gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt taggggaaat
    97741 ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat tggcaatggc
    97801 cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa tgcagcagga
    97861 cctacagcac accctgaagg actttatttg tatgaagtga aatatgaata agaggttatg
    97921 atagaagtgg tcagcttcaa gaaataagaa ggtatttacg aaaattactt ctcaaaattt
    97981 tgcaaaattt cggcttattt tcgaagaagc tacttctgga acaccgttta aggaagaggt
    98041 cgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cgggaaaata gctcctcata
    98101 ttttgccgtt tgttgagcta ataccgaagg tcagctgatt gaacaccgtt taagaaagag
    98161 gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca
    98221 tctttgatga atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta
    98281 cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac
    98341 agtctgaaca ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg
    98401 gttgttcttc tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc
    98461 agacataaag aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc
    98521 cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga
    98581 atgcgtcttt gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt
    98641 tgtcataagg aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag
    98701 cttttctgcc agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt
    98761 gataggctac aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc
    98821 cataaaaact tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt
    98881 attgaccaga aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac
    98941 tagaactttc taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg
    99001 caggcacatg gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag
    99061 tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat
    99121 agaatgtaaa atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg
    99181 tcaaattatc tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta
    99241 gtggcaatgc attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga
    99301 agccatattt actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc
    99361 catccatcag caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga
    99421 aacctaattt atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga
    99481 taccactgta accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc
    99541 ggtaaaacga gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag
    99601 gatcagtata taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac
    99661 tgttcgtatt gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat
    99721 tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa
    99781 ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc
    99841 cccatgccac cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccacgat
    99901 cgtcttgttc tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca
    99961 gataggcata acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca
   100021 atggaacaag ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat
   100081 ttttcaacgg tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac
   100141 ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt
   100201 tcatgattag atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg
   100261 cctcgccatt tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc
   100321 ctgccagtaa agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg
   100381 tcaatagacg ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa
   100441 aactggataa aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca
   100501 taacacgatg aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga
   100561 tgatctccga atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat
   100621 gcccccagcg aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc
   100681 cgaccttaag caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga
   100741 acaccagtgg cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc
   100801 ctagagaagc attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc
   100861 tggcatgaaa attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc
   100921 caggatagat tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac
   100981 tagctgctgc gtagcgtccg ttttctctaa agaatgactt taaatttttt ttcatattta
   101041 ttttccacca aatttctatg aagttgtatt cagtttatca tatccttgtt tttctctgct
   101101 taaaaagagt ctagttttca caaaaaaata aaatttagaa taaataactt cataatttgt
   101161 tcgtataaca aaaataaacg caaaaaaagg gtaggaatca aaagtcccac ccttaaaaat
   101221 ttttattaaa taaaaaaccg ttcactatct gaacggtcca agtatcattc aattaaacgc
   101281 gttcaacttt tcctgatttc aaagcacgag ttgacaccca aactttttta ggttttccgt
   101341 cgaccataac gcgaactttt tgtaagttag gtttaacagt acgttttgtt gagttcatag
   101401 cgtgtgaacg gttgtttcca ctttttgtct tgcgacctgt aaagtaacaa acttttgcca
   101461 tggtaatttc ctcctttgcc ttgatttgga gcaatagaat aattcagctc ataccagact
   101521 aacatatcat atagaatggg aatttgcaag tacattttca tagatacaat tgacaattga
   101581 gaagaataat tgtaaattaa acaaggttat acctgaaatt ttgcaaggtt tttgagtact
   101641 ttgggtaact gccttttatt tcaagttgcg ctatgataaa atatgataga agaaaaatgg
   101701 gagtttttta cccgttaagg aggctcattc atggctgtaa aaattcaaac gtcagctgga
   101761 accattgaaa ttaccaatga tgtaattgca acagttgttg gtggcgccgc gacagatgtg
   101821 ttcggtgttg taggaatggc cagcaaaaat caaattaaag ataatattac tgagattttg
   101881 cgtaaagaaa attactcaag aggagtagtt gtccgccaag aagataatgg tattgctgta
   101941 gatgtttata cgattatcag ctatggtacg aaaatctctg aagtttcccg caacgtccaa
   102001 gaaaaagtga aatacaatct agaggcatta ttaggcgtaa ctgccaattc agttaatgtc
   102061 tttgtacaag gtgttcgtgt actgccagac taggcaggag actggctggt aactcagcag
   102121 tcgatctgaa ggaggattat ctaggtgagt gtaacagaaa taagtgcaag ccagttccag
   102181 gaaatggtcc aggcaggtgc gaatcgtctg caagtaaatg cagaatacgt caattcatta
   102241 aatgtattcc ctgtgccaga tggcgatacg ggaacaaata tgaatctatc tatgactagt
   102301 ggagctaagg cagtagtaga ttcaacctca gaaaaagtag gagaattagc agcaatgctt
   102361 tcaaaaggat tgctgatggg tgcgcgcgga aattccggcg ttattttgtc tcaattgttt
   102421 cgtggatttt ctaagcaaat tccagatgtt gtctcattaa atgcttctga tcttgctgca
   102481 gccttcactc atggagttga aactgcgtat aaagcagtaa tgaaaccagt agaaggaacg
   102541 attttgacag tagctagaga agcagcgaaa gctggagaaa agaaagcaaa aagtactgat
   102601 gatgtcgttg aagtaatgac agctgttgta aaaggagcaa agcgtgcttt agctaaaact
   102661 ccagacttgc ttccagtcct aaaagaagtc ggtgtcgtag acagtggagg gcaaggactg
   102721 ctgtttgttt atgagggctt tttaagtgca ttgaatggag aattccaagc ggatgaaaca
   102781 tatgaacctt ctccagcaga aatggatgat atggtcaatg cagaacatca tcgcagtgtc
   102841 caaggtcaaa tggcgacaga agatatccat tttggttact gtacagaaat catggtaaaa
   102901 atcggagaag gacctacagt cgatagtaca ttcgattacg aagaattccg taactattta
   102961 gatggtatag gagattcttt acttgtggtc aatgatgacg agatcatcaa agtccacgtg
   103021 catacagaaa acccaggaga agtcttgaat tatggacaaa aattcggttc attagtcaaa
   103081 atcaaagtcg acaacatgcg cttgcagcat gagacgattt tggaacacga tgaagaagtg
   103141 aacgagtttg cgaaaaaaga aataccacgt atcccgtatg gtatcgtagc gatcgctgct
   103201 ggtaaaggcg tccaagagct gtttgaaagt ttaggcgcta attatgttat cagtggtgga
   103261 caaacaatga atccaagtac ggaagacatt gttaaagcga ttcaagaagt aaacgcagat
   103321 aaagtgattg tcttgccaaa caataagaac atcttcatgg cagctgatca agcagcagaa
   103381 gtagcagata tcccagtagc agttgttcct tcaagaacga tttcccaagg attgacagct
   103441 atgctttcat tcaatgatca agcttcttta gaagaaaaca aagaaacgat gacagaaatg
   103501 ttgtctagcg ttgtgagcgg tcaagtcact cacgcgatca gagatacctc aattgacggt
   103561 gtagagatca aagaaggaga ctttttgggc atgatcgatg gaaaaatcct gatctctaac
   103621 ccagatatca cagcaacagc gttaagtaca ctagaacaaa tgatcaatga ggacacagag
   103681 atcgtaacga tcttggcagg tgatgaagga acagttgaac aagcaaatga atttgctgag
   103741 aaactaacag aaaaacatcc tgatttggaa atcgagatcc accaaggaga tcagcctgtc
   103801 tatccttatc tattttctgc tgaataattt ctgtgtcaga atatctttct aacacagtat
   103861 tcattcataa aaggccttcg gttagtgatc aggtatgatc gctgaccaag tccttttttt
   103921 gttaaaggag ggaggaaaaa tttatgcgtt ctttagatga tccaatcact atgctggcag
   103981 gagtcggaga aaaaagagca gctagtcttg ccagcttggg catccaaacg attgaagact
   104041 tactgactta ttatcctttt cgctacgaag atattcagga aagaaatcta aacgagatcc
   104101 aggatcaaga aaaggttact ttgaaaggaa tcgtagtctc gcctcctgtg atgaatcgat
   104161 ttggttataa aaaaagccga ttgcaatttc gtatgatgca agaacatgat gtgttcaacg
   104221 tttctttttt taatcaacct tatctaaaag aaaaagtcat tttatcggag gaaatcgccg
   104281 tatacggtaa atgggatgca aagcgtaaag ccttgaacgg gatgaagatt ttaggttctc
   104341 aaacaatgga tgacttttcg ccgatatatc acgtcaacaa atcaattcgt caaacgacat
   104401 tagttgatct aatcaaaaaa gggtttcaag aatacggcac attgattgaa gaaaatctgc
   104461 caaacaacct tgtggagaaa tatcgactgc ttgaccgagc ttctgctgta agaagtatgc
   104521 attttccaaa agaccacgaa gaaaaccatc aagccaaacg acgtgtcgtt tttgaagagt
   104581 tctttttatt tcaaatgcgt cttcaaggat tgaaaaaagc agaaaaagca gaaacgaatg
   104641 gtttggagat cctttatgat gtccaaaaat tgaaacaatt tactcaaaag ctaccttttg
   104701 aactgacaaa tgcacaaaaa cgtgtaacga atgaaatctg tcgggatctt cgcagtccgc
   104761 agcatatgca gcgacttttg cagggagatg taggtagcgg aaaaacagtg gttgccgcga
   104821 ttgcgttata cgctaccgtt acagcaggtt tccaaggtgc gttgatggtg cctacagaaa
   104881 tcttggcaca acaacatatg gaaagcttgc aacagctgtt tgatcccaat gaagtaacca
   104941 tcgcattgat tacggggtca acgaaagcaa aagaacgacg tgagctcctt gaaaaactag
   105001 agcaagggga aattaacgtc gtgatcggca cacatgcatt gatccaagat ggagtcgaat
   105061 ttcagaattt aggtctagtt attacggatg agcagcaccg ttttggcgtg aaccagcgga
   105121 aagtacttcg tgaaaaaggc tggcgaccag atgtcttatt tatgacagcg actccgattc
   105181 ctagaacatt agctattaca gcatatggtg agatggatgt atctatcatt gatgaacttc
   105241 cagctggacg tatcccgatc gaaacacgct gggtaagaac acctcaattg gacagtgtac
   105301 tggattggac gaaaaaagaa ctggcaagag gacatcaaat gtatgtcatc tgtccgctga
   105361 ttgaagaatc agaagcatta gatgtaaaaa atgcagtgga aatctatgaa aaacttcgtg
   105421 aactatttgc accaaaatat gaagttggct tgttacatgg taaaatgaag aatcaggaaa
   105481 aagaagtaat catggaagcc ttcaaagaca accagatgca gatattagtt tcgactactg
   105541 taattgaagt aggggtaaac gtgccgaacg cgaccgtcat gctgatcatg gatgcggatc
   105601 gatttggatt ggcacagctg caccaacttc gtggacgtgt tggacgcgga agtgatgctt
   105661 cctactgtat cttagttgct aatcctaaaa atgaactagg tgtggaacgg atgaaaatca
   105721 tgacagagac caccaatggt tttgtactaa gcgaaaaaga tctggaatta cgtggtcctg
   105781 gtgaagtatt tggcttccgt caatctggtc tgcctcaatt tgcgattgct gacttggtga
   105841 cggatggaaa tgttttggag gtagccagag aagaggctgc agccctttgg caaataaagg
   105901 attggcagat gcttcctgag tatcagcctt tagcgcaaag cctccaaata gaaaatcagg
   105961 ataatcgtta tttcgattag cagactgtgc tatactttat actgatgacg gaaaaaggag
   106021 tggaaacaat gaaaattgcc gtagatgcaa tgggcggaga tcacgcacct caagcaattg
   106081 tagaaggcgt ggctttagcg cagcaagatt ttccagaagt agaattctta ctttacggaa
   106141 aagaagcaga aatcaagaaa tacttaacaa atgaaaaaaa catcacgatt atccatacag
   106201 acgaaaaaat caatagtgat gatgagccag taaaagcaat tcgacgaaaa aagactgcat
   106261 caatggtcct tgctgcacaa gcagtgaaaa acggtgaagc agatgctgta ttttcagcag
   106321 ggaatacagg cgcgctacta gcagcagggt tgttcatcgt aggacgtatc aaaaatatcg
   106381 aacgccctgg cttgatgtct accttgccag tagtagggaa agaaggtgcc ggtttcgata
   106441 tgctggatct tggcgctaac gcagaaaaca aaccagaaca tttgttgcaa tacggtattt
   106501 taggttcttt ttatgcacgt aaagtccgag gtatcggacg tcctcgtgtc gcactcttaa
   106561 acaacggaac agaagcaaca aaaggtagtg aagtgacgaa aaaagcctat gaactccttc
   106621 agaatgaaac aagcttgaat tttattggaa acgtagaagc tcgtgagcta ctcaatggtg
   106681 tggctgatgt agtggtcaca gatggattta caggcaacgc tgttttgaaa tctattgaag
   106741 gaacagcaat gaatatgatg aatctattaa aatctgctat tctaaatgaa gggatcaaag
   106801 gaaaaatggg cgctatgctt ttaaaagacg gcttacgttc tttgaaagca gaaatggatt
   106861 attccaaaca tggcggtgcc gttttgtttg gcttgaaagc accagtcatt aaaactcatg
   106921 gagcaacagg ccccgatgct gttcgctata cgatccgcca aatccacaca atgcttgaga
   106981 cagatgtagt tggacaatta gtcgaacaat ttgaaaaaag cgaagaatag tatttggata
   107041 tctgaagcga tccgttccat tgaaaaaaag gtggacattc ctgaaaaatg ccagtaaaat
   107101 tagccatggt gtaggtaatt gacaagtgta tgattcttgg aggtgaaaaa gttgactcgc
   107161 gaagaagtat ttaacaaagt agcgaaaatt atttcaaatc attttgagat agataccgat
   107221 aaagtgacgg atgaattgaa catcaaagat gatttaaaag ctgattcaat tagtattatg
   107281 gaattcgttt tagagcttga agatgaattc ggtacggaaa tttcagatga agacgctgaa
   107341 caaattgaaa cagtcggtgg cgcagtagat tatatcagca gccatcttaa ataatttcaa
   107401 ataaaagcaa agaaggataa aaagctgcat gcagcttttt atccttcttt ttttaatctt
   107461 tttgacacgt aaaagtcatt tttctgataa ttctggcgat aaacagaaat tatgttgcaa
   107521 agtaaagcgg ttttctatat aattattaat aattcattac acaaagataa gaataacctt
   107581 tgttataaaa cgatttattc tgtgtaatgt ccttttttat taagaaaaac ttccatttag
   107641 gaagcagaga ggggtattcg attgtccgat aaccaattat tagaagttca gaacctacat
   107701 acaggattcc gactcaaaga tgactattac gatgcagtag atgacgtttc atttacttta
   107761 gacaaaaacg agattttagc aatcgttggg gaatctgggt gcgggaaaag taccttggct
   107821 actacgatta tgggattact tgatcctaat aacacaaaaa ttacaggtga gattttatat
   107881 aacgatctga atttaatcga cctgaacgaa acgctctata ataaaatccg cggaaatgat
   107941 atcggaatga ttttccaaga tccgttgtct gcattaaatc ctctcatgag aatcgaagac
   108001 cagatcaaag aaggactttc ctatcacaca aagatgaatg cagaacagcg tcaagctcgt
   108061 gcactagaat tgctagaaca agtagggatt cctaatcctg cgagagttgg acgccaatat
   108121 ccccatgaac tatcaggcgg tatgagacaa cgtgtcatca tcgcaatcgc aatcgcctgc
   108181 aaacctccaa tcgtcattgc agatgaaccg acgacagccc tagatgtaac catccaagca
   108241 cagattcttg atttactgaa agaccttcag gaagaaacaa aaacagggat catcttgatc
   108301 actcatgact taggagtcgt agcagaaatg gctgatcgag tggcagtgat gtatggcgga
   108361 cagttcgttg aagtagcagg tgtaaaagaa ctgtttgatc atcctaagca tccttataca
   108421 cgctctttac tgaactctat accacaagaa ggaaatcatg atgcagaact ccatgtgatc
   108481 gaaggtgtcg ttccttctct gaaaaacatg cctcgtaaag gttgtcggtt tgcaccacgt
   108541 attccatgga taccagcaag cgatcatgaa gaagagccta tccttcatga agtcgcaccg
   108601 aaccacttgg tacgttgctc ctgctataag catttccact ttagagacga aaaaggagag
   108661 gtgtagatga cagaattaat cacaatcaaa gacctgaaag ttcattaccc tattcgcagt
   108721 ggattcttca atcgagtaac ggatcatgta tatgctgtag atggtgtgga ttttattatc
   108781 gaaaaaggaa agacatatgg tttagtggga gagtctggct ctggaaaatc tactacggga
   108841 aaagccgttg tgggattaga aaaggtaaca gccggacaaa tcatgtatga aggaaaagat
   108901 gtaacgaagc gaagcaaccg caaaaaaatg ggttataaca aggatgtgca gatgatcttc
   108961 caagattcaa tgtccagttt gaatcctaaa aagcgagtgt tggatatcat tgctgaaccc
   109021 atccgaaatt ttgaacggtt aagcgatcaa gaagaaaaga aaaaggtaaa aggactgcta
   109081 gatatcgttg ggatgccgga agatgcgcta tataaatatc ctcatgaatt ttcaggcgga
   109141 caacgtcaac gtctaggagt agcccgtgct gttgctacca atccaaaact gatcgtagcc
   109201 gatgaacctg tatctgctct ggatctatct gttcaagcgc aagtattgaa tttcatgaaa
   109261 cgaatccagc aagaatacgg cctcagctat ttattcattt cacatgattt aggtgtagta
   109321 aaacacatgt gcgacaatat cgcaattatg tacaaaggtc gatttgtaga aatcggtaca
   109381 agagaagaca tttataatga cccgcgacat atctatacga aacgtctgct ttcagctatt
   109441 ccgcaaatcg atgtagaaaa tagggaaagc cacaaagaaa atcgacgaag agtagaacaa
   109501 gagtatatcc aaaaccaaaa agaatactat gatgcgactg gtcgagtcta cgatttgcgt
   109561 acgatcacaa ctacacacaa agtggcgttg aaagatggag gtgcaagcta atgtggaaaa
   109621 cgattctgcg acgtgtttta ttgatgatcc cgcaagttat tattttaagt gttctgatct
   109681 tcttattagc ccaagcgatg ccgggggatc catttaccgg tttgatcaat ccaaatcagg
   109741 atccggcagt gatcgaacag atgcgacaag cagctggctt gaatgaccca tggtatgtcc
   109801 agtacttccg ttggatcgga aatgctttgc aaggcgattt tggtcaaagt ttcttataca
   109861 aacgctctgt tgctgacttg atcggcgaac ggatcatcaa cactttatat ctatcgatct
   109921 tgacagttgt ccttacttat ttgattgctg tgcctctggg gatgcttgct ggccggtatc
   109981 aaaattcaat cttggataag attgtcgtaa tctacaactt ctttagtttt gctgttcctt
   110041 tatttatctt cggcttgatc atgctatttg tttttggtta tcgtttaggc tggttcccaa
   110101 caagtggatc acaaacacta ggtttgcaag gtggatttat cactcaatgg atagaccgtt
   110161 tgaaatatat tctgttacca tcaattaccc aagcattttt ggcgactgcc gtcacaatcc
   110221 aatatctgcg aagtgaagta atcgattcga aatcgctcga ttttgtcaga acagcccgtt
   110281 ccaaaggggt accgacgaat aaagtattca gtcgacatat tttccgaaat gcagcgttgc
   110341 cgatggcttc tcaaatgggg tatgaaatca cttcgttgat tgctggttct gtagttatcg
   110401 aaaagatttt tggttatccg ggagtcggaa aactatttat tgattccatc ggccaacggg
   110461 actataccgt gatcacatcc ttagtattga ttttaggaat cgcgacgcta gtggggacat
   110521 tactttcaga tatcatcatg agtatcgttg acccgcgtat tcggattcaa taaaaccaat
   110581 cagaaaggag aatggatcga tgagtgaaaa aaacaataca gcagttcaag aaagtattcc
   110641 tcctatgggg atacgaatga ttgctcgaga atttaaaaaa gataaaattg ctattttttc
   110701 attaggtttg ctagtggttt tattattagt gatctttatc ggtgcgatgc tgaccgatca
   110761 agacaaagtg atgtatgtta gtatcttcga caaatacgct gcaccaggaa cagtttcgac
   110821 acaaggacaa aagttcattc tgggagcaga tgaaggcgga cgtgatgtat taggacaatt
   110881 gatcataggt gctagaaact ctgttacgat cggatttgcg atcacgatcc ttacttcgat
   110941 catcggggtt ggattaggga tcatctctgg ctactacgga gggatcattg acaacatctt
   111001 gatgcggatc gttgatttta tcatgattct accgatcatg ctgatcatta tcgtcttcgt
   111061 atcgattatt tccagataca atgtctggtc ctttatattc atcatgagtg cattttattg
   111121 ggtagctaaa gcccgattat tccgaagcaa gacattatct gaagcaagaa gagattatgt
   111181 cagtgcctca aaaacaatgg gaaccaatga ttttaaaatc atgttccgcg aaatcatgcc
   111241 gaatctaagt tcattaatca ttactaattt gacgatgaac tttgcggcaa acatcggtat
   111301 cgagacaact ttgactttct taggtttcgg cttgccggca aatgtaccta gtttaggtac
   111361 attgatcggt tatgcaagta acggagaagt cttagtgaat cggcaatgga tctggttacc
   111421 cgcatcaatc cttattttag ttttgatgtt gagtatcaat tatgttggac aagcattcaa
   111481 gcgttctgca gatgcgcgcc aacggttagg ctagtataga agcgaagggg gaaataggaa
   111541 tgaagaaaac gatttttgga tttgcagcgc tgatgtctgc tgtcgttctt gcagcatgcg
   111601 ggggcggagg tacagatacc tctggaaaca actctggagg caacggcaat aacaaagagg
   111661 aattaacttt tccaattgca acaaaaaata atgatgatgc aatcgaagac ggcgaattag
   111721 aagctgcggt cgcaacggac agtcagttta aaggattgtt ccagtgggag ttttaccaag
   111781 attcatatga tgcagctttt atggctcctt ctcatgaacc gctatttgca aacgatgacg
   111841 atttcagaat cacagacgac ggagctgcaa cgcttgatct agatgaagat acgaaaaaag
   111901 cgacaatcaa agtaaaagac aacgtcaaat ggtcagatgg tgaagatgta acggcggacg
   111961 atgtgatttt cccttacgaa atcattggga ataaagatta cactggtatc cgttatgacg
   112021 acactttccg taacatcgta ggtatggaag aatatcatga tggaagtgcc gacacaatct
   112081 ccggaatcaa aaaaatagac gacaaaacag ttgaaatcga atataaagaa gtaaacccaa
   112141 gcatgcttca atcgggtggt ggtatctggt catatgcagc accaaaacat acgttagaag
   112201 gtgtagcaat caaagatatg gaatcaagcg atcaagtacg taaaaatcca gtgacttttg
   112261 gcccttatta catgagtaat atcgttgcag gagaatcagt agaatacttg ccaaacgagc
   112321 actactggaa tggtacgcct aaattgaaaa agatcacgat gaaatctgta ccgatggcaa
   112381 gtgcaactga agcattgaac tctaaattat atgatatgat tttccaaatg ccaacagata
   112441 cttacgatac ctataaagaa attgctggct atacaaactt aggccgtgaa caaacttctt
   112501 atacttatct aggattcaag ttaggtaaat ggaatgcaga tacaaagagt gtggaataca
   112561 atccagattc aaaaatggct gataaatcat tacgtcaagc aatgggctat gcattagata
   112621 ataatgcagt aggtgaacgt ttctatgcag gtttgagaag caatgcgact tcattgattc
   112681 ctccagtatt ccagtcatac catgatgatg agatgaaagg attcacacaa gatctagaca
   112741 aagcgaaaaa actattagat gatgctggct acaaagatgt ggatgacgat ggtttaagag
   112801 aagacaaaga tggaaataaa ctaacaatca actttgcttc gatgtccggt ggggaaacag
   112861 cgcaaccatt ggcagattac tatatccaac aatggaaaga aattggtttg gatgtccaat
   112921 acacaacagg tcgtctgatc gaattcaatt ctttctatga taaattagaa aacgatgatc
   112981 ctgaaattga tgtttaccaa gctgcttgga atacaggaac tgatccaaat ccaagtggac
   113041 tatggggacc aaatgctgcg ttcaactaca ctcgttatga atcagaagaa aatacaaaat
   113101 tgatcaacga tatcagttca agcaaagcat ttgatgaaga gtaccaaaaa gaagcatatc
   113161 aaaaatggca acaatatgca tttgaagaag catttgcgat tccaacgttg ttccgtaatg
   113221 aagtattgcc agtcaacgac cgtgtcaaag actgggattg gtcttatgat gtaccaaacc
   113281 catgggcaaa tgtttctgtt acagcagatt cacgttcata atcactaaaa atgaccgaaa
   113341 aagggcgatg tcctttttcg gttttttgat atttttctgt ttatttctct aatattcagg
   113401 tcaaggcgct gatttcatga cttctccaat aaacggtgtt caaagtccag tttctcggta
   113461 ttaagtcaaa tttatgcaaa aatatggaaa gccattttca caaaattttt tcttcatact
   113521 cgaaactaaa cgaatttttc acaacttctc gtcaatcact aggattatta aagctttact
   113581 taccttttga ccgaaccatt tcaaatttga caaaaaacca gtataataag gaagtatcga
   113641 aaaaatatcc ataaaaggaa tgtggaaaat ggacaaccag ctgacaaaag aactaaaaga
   113701 aaactataac atcgtttttc acgatctaaa tttgcttgaa caagctttta cccattcatc
   113761 ctatgtgaat gagcatcgca atctgcaatt atcggataat gagcgacttg aatttttagg
   113821 agatgccgtt ttagaactga tggtttctca gtatttattc cgtttatatc ctcatttgcc
   113881 agaaggaaaa ttgacaaaaa cacgagctgc gatcgttcgc gaagacagct tgtcgaaatt
   113941 tgccaaagaa tgtcactttg atcaatatgt tttgttgggc aagggagaag agaattcagg
   114001 aggacgtacg cgaccagcat tgctttgcga cctattcgaa gcttttttag gcgcgttata
   114061 tcttgaccag ggattcgaca cgacccatgc attcatcgaa aaagtgattt ttcctaaagt
   114121 caaagcaggt gctttttcac atgagatgga tcataaaaca aaactacaag aagtcttgca
   114181 aaaatcaggt gatgtatcaa tcgaataccg attgatcaat gaagaaggtc cagctcatga
   114241 acgagtattt tggatcgaag tttatgtaga tgatcaattg attggcactg gacaagggaa
   114301 atcgaaaaaa ttagcagaac aagctgcggc ggaaaatgca ttggcagcac tgtaaaagta
   114361 agtgagggaa agctttgtat ttgaaacgaa ttgaaatagc cggttttaaa tcatttgcag
   114421 acaagacagt cattgatttt gaaaatagcg tcacagcagt ggttggacca aatggcagcg
   114481 gaaaaagcaa tattacagag gctatccgct gggtattagg agaacaatct gctaaaagtc
   114541 tgcgaggcgg aaaaatgccg gatatcatct ttgccggttc cgatacccgt aagcagctga
   114601 atattgcaga agtcacagtg attttagaca atagtgatca ttatctgcca ttggagtaca
   114661 atgagatcag tgtaacccgc cgttatcgac ggactgggga aagcgagttt ttcatcaata
   114721 aacaaagctg cagattaaaa gatatccaag aattgttctt agattctgga ttaggaaaag
   114781 aatcgttctc aatcatttct caaggaaaag tagaagcgat tttcagtagt aaaccggaag
   114841 accgtcgtgg gatctttgaa gaagcagctg gtgtcttgaa atacaagcag cggaaaaaga
   114901 aagcagaaca gaagcttttt gaaacagaag acaacctcag ccgcgtccaa gacatcatcc
   114961 acgaattgga agaacagtta actcctttgg ccgcccagag tgaggcagca aaggagtttt
   115021 tacggctaaa agaaacactg actcaaaccg atgtctcttt gatggttgct gaaatcaaga
   115081 cagcaaaaaa agattgggac aacaaacaag cacagcttgc gaaattcaat ctagaattag
   115141 gcaaactatc tgagtcgatc caagaacaag aatccatttt ggcgaagcag cgtaaagaaa
   115201 atgcgcaagc cgatcgtctg atcgaaaaaa atcagcaagt attattagat ctttcggaaa
   115261 aactgaagca gacagaagga caaaaagatg ttttgcagga gcggacaaag catacacaaa
   115321 aaagctcgca agaatatcaa acttcattag cagaagcaca aaaaaaggtt aaacattttg
   115381 aaaagcttca agaaagcttg atgaaagcag cagcagaaaa agaaacagag atccaaaaag
   115441 ctgaagcaaa tctcataaaa acacagcaag aattagaaaa ataccaaaaa tcaacgaaag
   115501 aattattggc agaattaaga gatcaatatg ttgatttgat gcaagaacaa gcagctgtag
   115561 gaaacgaact gaagtatcta gaacgtcaat atatacaaga aacagcaaaa agcaagcaga
   115621 cgttagccaa gcaatcggaa gtagaagcat cagtagaccg attgatctta caaaaagaag
   115681 aattgacaca aaagcaggcg cagctaaaat catctttaac tgaaacaaaa gagaagttag
   115741 aaatgatcca acaaaacggg aaaaagttcc aagaagcatt agcaaaagaa cagcctaaaa
   115801 tgtatcaatt aatgaatcaa gtccagcagc tacgtgctcg taagaaaagc cttcaagaaa
   115861 tccaagaaaa ctattttggt ttttaccaag gggttcgatt ggtcttgcag cataaacagc
   115921 agctttcggg tattgtcgga gcagtggctg aactgatcga tgttcctgca gatttcacgt
   115981 tagctatcga aacagcttta ggtggggcag cccagcatgt catcgttgaa aatgaaaaag
   116041 atgctcggca agccatcacg tatttgaaac agcaaagagg aggaagagca acattccttc
   116101 cactgacgac aatcaaacca agacaattac cggctcatat attgacacaa gcagcagctg
   116161 tagaagggtt tatagggatt gccagtgaac aagtcagtta tcccgatcaa atccaaactg
   116221 tggttcaaaa cctgctagga acgatccttt tagccaaaga cttaactagt gccaatgcaa
   116281 tcgcgcaaac gatccgctat caatatcgag tggtttcctt agaaggtgat gtgatgaatg
   116341 ccggaggatc catgacaggt ggagcgaata aacgaggaaa ccaaggcagt ttgtttgtac
   116401 aaaatcaaga attgaaacag ttgactagtg aatttgaaga agcagataaa caactccaag
   116461 cacaagaaaa aaaagttcag gagctgcaac aagaaacagc tcgtttagcc gaagaacaag
   116521 aagttttgcg tacacgaggc gaacaattac gttttgaaga acaagaagcg acaaatcagc
   116581 tacaaaacat cataaatgaa cttgaacgtt ttgaaaaaga aaagcaaatc tcaacttttg
   116641 agacacgtga actgcagcag tttatcgaag attacgaaaa gcaaacgaac gaattgaaag
   116701 acaaacaaac agatcttgaa tcgcaacgac aaaaaatcga cgaagaaatc aaatcgttaa
   116761 gtcaagaaag cgaccagatg gaagccagaa gagcacaagt acaaagtcaa aaagcacaag
   116821 aacaagcaga tcttgctgtc ttgaaagagc agtttaatca tctgcagatc cagctccgcg
   116881 gtgcccgagt ccaaaaagca gaggcaaccg aacgtcaaga agcgatagaa aagcagttgg
   116941 cgacattgac agcagacttt tctgatcatg aagtcacaga agaaagtttg gaaaaacaaa
   117001 ttaacgaatt atctgctcag cgagaaacat taaaagccga gttggcaaaa gccaaagagc
   117061 agcgagatag aaggcaaaag gagatcgata agcttgaagc agtcttggca gagcgtaatc
   117121 gtgagcaaaa agcacgactg tcggaacaat ccaaattaga agtccaaaaa gaccgagctg
   117181 aaatgatttt agataatcat ttgacctatc tgcagtcaga atatcagctg acatttgaga
   117241 aagcttccca agactatcaa gaaacaaccg atatcgaaga cagtagaaca aaggtttcat
   117301 ccttaaaaga acagattgaa aaactgggac cagtaaactt aaattcaatc gaacaatacg
   117361 aacaagtcag tgaaagacat acattcttag caacacagcg agatgatctg ttagcagcga
   117421 aaaaccaatt gtttgaaacg atggatgaga tggatgatga agtgcggaca agattcaaag
   117481 aagtatttga agctatccgt caagaattca aaattgtctt tccaaatatg tttggcggcg
   117541 gtaaagctga attagtactg acggaaccaa ctgatttgct gaaaacaggt attgagattg
   117601 aagtacaacc tcctggcaaa aaactgcaaa gtctaagttt gctttctgga ggagaacgag
   117661 cacttacagc catcgcgttg ctcttttcca ttatccgtgt ctgtcctgtt ccgttttgta
   117721 tcttagatga ggtagaagca gcgttagatg aagccaacgt gaaacgtttt ggtcgttact
   117781 tgagcgattt ccaagatgac acacaattta tcgtagtcac acaccgcaaa ggaacgatgg
   117841 tcgcagcaga tgtactgtac ggagtgacga tgcaggaatc cggtgtatca aaaatcgttt
   117901 ctgtacggat ggaagatatc aatgaagagg cgaaaatcaa cgaaacgaaa gtgtaggaac
   117961 gaaaatgatc aaattagtag caatcgattt ggacggtaca ttattaaatg aacaaaagct
   118021 gatttcagat gagaataaac aagcattagc acaagcaaaa cagcaagggg tcaagatcgt
   118081 tctatgtaca ggacgcccct tagctgcgat ggcgcattat ctgcaagaat taggactggt
   118141 agatgaaggt gactacagca tcactttcaa cggcggtttg gtacaaaaaa atgatactgg
   118201 tgaaatcatg gaaaagaaag tgatggaagt atcagatatc caacgcttat acaaactggc
   118261 tcagcaattg gatctgccac tagatgtttt gtctgacaat gtggttttac agctgcctag
   118321 tgccccaagg aagcaatcct tgtacaatgt gttgaataag ctgctgcaat tccaaccagc
   118381 ggatataagc gatattacag atgaatttgt gcttaataag gcagtaatcg cttatgacca
   118441 agaagaatta gatcctaaaa tcaaagaaat accagcagaa ttccatgaaa tgtatgagat
   118501 catcaaaaca agaagtatgc ttttggaatt tatgccaaaa ggtgttacaa aagcttatgg
   118561 tatttcttta ttggcaaaag atcttggttt gaaacaagaa gaaatcatgg cgatcggtga
   118621 tgaggaaaat gatttaccga tgatccaata tgctggacta ggcgtagcga tggagaatgc
   118681 tgtgcctttc gtcaaagaag cagcagattt cgtgacagcc agcaatgaag aaaatggcgt
   118741 agccaaagtg atccaaaaat ttgtattaga gtagggggag tcaaatggga ttttttgata
   118801 aaatcaagcg tgcttttact ggcgaagaga aaacagaaga agttgaagta caggagaagt
   118861 acgacaaggg tttagaaaag acgcgtaaaa catttggtga acggatgaat gagttgtttg
   118921 ccaattttcg aacagtcgat gaagactttt ttgaagaatt agaagaaaca cttatcggtg
   118981 cagatgtagg ttttgaaaca gcgatacgaa tcaccgaagc attacgtcaa gaagtgaaac
   119041 tcagaaatgc gaaaaaatcg gcagaagtcc aaaatgcgat catcgagaaa cttgtggatc
   119101 tctatgaagc ggaaggaatc aatgaagtaa atgaactaaa tatccaagaa gacggactaa
   119161 cagttatttt gtttgtcggt gttaatggtg tagggaaaac aacaagtatt ggtaaacttg
   119221 cccatcagtt caaaaacgaa ggtaaaaaag tattgatggc agcagcagat accttccgcg
   119281 ctggagcaat cgaccagtta gttgtctggg gcgaacgcgc tggtgttgaa gttgttcgtg
   119341 gcaatgcagg aggcgatcca gcagctgtcg tattcgatgc tttggaaaga gcaaagacag
   119401 agcaggcaga tgttttacta gttgatactg caggccgact gcaaaataaa gtgaacttga
   119461 tgaacgaatt agaaaaaata aaacgagtaa tccagcggga attaccagaa gcaccccaag
   119521 aaacacttct tgtattagat gcaaccactg gtcaaaacgc gatggttcaa gcaaaacaat
   119581 tcaaagaaac aacagatgtg accggtcttg ttttgacaaa attagatggt acagcaaagg
   119641 gcggtatcgt acttgctatc cgtaatgaat tgcatttacc agtgaaattg gtcggtcttg
   119701 gtgaaggaat cgatgacttg gagccgtttg atccaaatga ttttgtcgtt ggattgttca
   119761 aaggattact aaaagaagaa taacccatgt ctaaaaagca ggagctgtga caaaagttgc
   119821 agctccttgt ttgaatgatt gtttttaaca tagctgtgtt tttgatgcct tcatttttat
   119881 acttatttgt aagcggttct caaacaattt cgttcttttt cttagacttt ttgattataa
   119941 ttagattcat atgaattgga ggtcacttaa tgatgaattt taagcaatta ttattgcatg
   120001 tcaatgcgcg cccttttatc gaccaagctc gatttggtat agaacgggaa ggacagcggg
   120061 ttgatcttgc aggaaatcta gcaaaaaccg atcatccagc aatctttggc gatcgatcct
   120121 atcaccccta tatccaaaca gattttagcg aaacacaaac agagatgatc acccctgtta
   120181 ccgattctat tcccgaatta tttcagtatc tcgctgctgt ttatgatgtg actgctcgtt
   120241 ctataccgaa agaagagatg atctggccat taagtatgcc acctgcctta ccggaaaaag
   120301 acgaagagat cattattgca aaattaaaaa atttcgaaga tgtcttgtat cgacgttatt
   120361 tagcaaaaga atacgggaag cgaaaacaga tggtaagcgg tattcatttc aattttgaat
   120421 ttggcgatga gttgctaaga acattgttca gccatcagga agaatttcaa gatttttccg
   120481 aattcaaaac agaactttat ttgaaaacag cgagaaactt tatgcgctat cgctggatga
   120541 tcacttattt attcggtgct tcaccaatga gtgagaagaa ctacttttta gatgaatcac
   120601 atccgcaaga acctgttcgc agtattcgca acagtgcgct gggctatacg aatcatccaa
   120661 atgtgaaagt ttcctatgct tctatgaaac agtatttagc agatatcgag cgaatgatcg
   120721 aagaagggaa actttcggag gagaaagaat tttatacacc gcttcgtttc cgaggcggaa
   120781 agaaagtcgc agatttagca acaacaggtg ttcgttatat tgaattgcgg aatatcgatt
   120841 tgaatcctta tgcaagattg ggtatcaatc cagagcaagt tcggttctta caattattcc
   120901 tgatgtacat gttatggaca gaagaaaaag aagactgtga ccaatgggta gcagaaggta
   120961 cgactcgaaa taacaaagta gcactagaac aaccatcaga tcaaacagaa tttcatcaag
   121021 aaggtagaga aattcttgaa ggaatgaagc aaatgctggt tgaattggat tggttggatt
   121081 ctctttattt agtagaggaa gcattgactc aaatggatca tcctgaacaa acgttagcag
   121141 cgaaactcta tcaagaagca cagctatcga gtcagcaaga agttgctgtg gcattgggac
   121201 atcaatatta taaagaaagc catgaacgtc cctatcagtt ggccggtttt cgcgaaatgg
   121261 aactttctac tcagatattt atgttcgatg ccatccaaaa aggtgtccaa gtcaaagtat
   121321 tagatgagtc ggatcaattt ttgcgtctgc aattccaaga ccatgtagaa tatgtgaaaa
   121381 atgcaaacat gacaagtaag gacagctata tcgtcccact tatcatggaa aacaaaacag
   121441 taacgaaaaa agtcttaaaa gaagctgggt tccgggtacc aggaggcgca gaattttcat
   121501 ccatggaaga agcggtaaaa gcttatccga gatttgccga gcaggcattt gtgatcaaac
   121561 ctaaatcaac caattacggg ttaggtatca cgattttcaa ggaaggagct agtctggaag
   121621 attatcaagc aggtttagcc attgcttttc gtgaagacag ttcggtttta gtagaagaat
   121681 ttatgccggg aacggaatat cgtttctttg tgatcgacgg agaagtccaa gccatcatgt
   121741 tgcgagtccc tgctaatgtt attggtgaca gtatccgaac agtgaaagaa cttgttgaag
   121801 aaaaaaatag tgaccctttg cgtggaacga atcatcgtgc acctttagaa ttgatccaat
   121861 taggtgaatt agaacaattg atgttgaaag aacaagggtt gacgatcgaa tcagttcccc
   121921 aagccaatca aattgtttat ctaagggaga actcaaatat cagtacaggt ggtgattcga
   121981 tcgatatgac tgatgaattt tctgaagctt acaaaaaaat cgccgtttct gcggtagaag
   122041 cactaggagc taagattagt ggtatcgatt tgattatacc agataaagaa atcgatccta
   122101 caactgataa aaaagcatat gggatcatcg aagcaaattt caatccagcc atgcatatgc
   122161 atgtttatcc ttttgcagga aaaggcagaa gattgacgat gaatgtattg aaattgttat
   122221 atcctgaagt attttaaaag aaaaagcttt tgacagattg tcctatttga tgtcaatctg
   122281 atcaaaagct ttttttgcat gtaaaaattt atgtgttttt tcgttttaaa ataaacgatc
   122341 ccagaagctt tttttctttt ttggtttttc tttttcagct ggcgaagagt tttcttctgg
   122401 gtattttttt tcgatatcaa tcacaggttc atttactgct tcttttgctg ttaagagcaa
   122461 tccgaattcc tcaggatttg cagtgacgta atcgttgact acagtgaaac ggctattgtt
   122521 tttcgttgct agctggatat attggttttg gacagaaagg ggtagagccc cattcaagag
   122581 tatggaagca tctggatgtt ttttgagttc ttttaaaaag ttttctttgt tttttggttg
   122641 gatcatttca gcaacagtca tactaagata gcagcgttcc ctgaatgtcc ctaaatattt
   122701 atgctgttct tcagggttca ctaaaggtgt accataaagt cccttatcca aatggctttt
   122761 tacatcatct tgtgccataa aaaataccct ccttcttctc tttgtctttc ttacgattat
   122821 agcatattcc caataatacc taaaacatat gaaaaagcgg ttgcttttgt tcaacgattc
   122881 acttaaaata agaatgaaaa tcattctcaa ttagaaaggt ggattggatg cgctacggac
   122941 gactagtagc attattagtt attttaagta gtatttcatt gttcatcgga gtatcgaatg
   123001 tacaattgtc agaactgctc cagttagatg ggcaaagctt acaaatcttg ctagtgactc
   123061 gtttgccacg aacgatttgt ttgatccttg tgggagcgac cagtagtatt tgtgggttga
   123121 tcatgcaaca tttgacccaa aataaatttg tttcaccaac gactgcgggc acgatggata
   123181 gtgcgcgttt aggtatttta gtggctatga ttttcctacc tggtgcatcc ttgattgtcc
   123241 gatccttgac agcattttgc tttgcttttg caggaacatt gcttttttta tcccttacgc
   123301 gattgttcct ccagaaaaat caattgatgc ttccgttgat tggagtcatg ttagggaata
   123361 tcattggttc agttgctact ttttttgctt atcaattcca gttggtacaa aatatgactt
   123421 catggcttca agggaatttt gcgactgtga tacgaggaaa ttatgagtta ttgtatgctg
   123481 ttgttccatt gttgctcttg gtttggctat ttgcttaccg atttacgatc attggtctgg
   123541 gcgaatcgtt tgcagtaaat ctgggaatag attaccagaa gatgcagttt ttaggcattg
   123601 gcttagtatc attggctagt gcagtcatgt tgatcatggt aggaagcatt ccttttatcg
   123661 gagtgatcat tccgaatctt gtctctagaa tatatggaga tcaagtgcac aaaacaattg
   123721 gcgtaactgc tttagcaggt agtttgttct tagtattttg cgatcttgtc gctcgcgtgt
   123781 tgatttttcc ttatgaaata ccagtcagtg tgatcgttgg aatcatcggt ggggctatct
   123841 tcctttatct tctgataagg ggacgacgat atgcttaaaa aaaagccatc tatttttatt
   123901 ggtatgcttg ttcttgtttt atttgttaca ggaatgtact tgacgatcca tacatatgga
   123961 aactggcatt ttgcatgtgc attgaggagt aagaaaattt ttgcatttat ccttgtctcg
   124021 ctagctgtca gcagtgccac aatcagcttt cagacaatta cgcataacca gtttttgact
   124081 ccttcagtat tgggacttga gtcgttatat gtccttgtcc aaacggtcct tttcttcttt
   124141 atcggaggag tgacgatgct tcagcagaca ggtacagttt ttttcttatt gaacgttggg
   124201 ctgatggtcg ggctgagtct attgttattt ttctttcttt tgaaaaaaat gagagcgaat
   124261 ctttttttat tactgatgtc agggatgatt ttaggtacgc tttttggtag tgtcagtacc
   124321 tttttacaag tcatcatgga tccgaatgaa tatgatttgc ttcaaggcag attatttgca
   124381 agctttggca atatccagag tcagtatctt ttgcctgctt ctttgatcat tatcggctgc
   124441 tggtacctgt tatttcgata tcgacatgtg ctagacgttt tccatctcgg aaatgaacaa
   124501 gcagtaaacc tcgggataga cacagatcgt tttgaatttt tgattttatg ttgtgtcagt
   124561 cttttgacag ggacatcaac ggctcttgtg ggtccagtta cttttttagg gtttattgtc
   124621 gcaaatatca gttatcgtgt cttttctact tatcgtcatg gtcagctatt tatcggaagc
   124681 tttctggtag ctatgctgtt tctgacagga ggacagctcg cagtagagca gattttcggc
   124741 tggaatacaa cagtgagtgt cgtcgttgaa tttgtcggag gaatttattt tgtcacaaaa
   124801 ttgattcgcg aaaggaagag tatcctatga ttgatttaca aaaggtcaat aaactgtacc
   124861 atggaaaatc agtggttaaa gatgtacagt taacgattcc tgaaaagaag ttgactgctt
   124921 ttatcggtcc aaatggtgct ggaaagtcaa cggtgttgtc gatgatcagt cgtttgatac
   124981 caaaagatac aggtgaaatc tatttagacc ataacgaagt aaaggcttgg aaatccaatg
   125041 aattagccaa aaaattatcg atcctacgac aatcgaatga aataaatgtc caaatcaccg
   125101 tgaaagaatt agtagaattt ggacgatttc cttatacgaa aggaaaattg acgacaaaag
   125161 acgaggaaat cgttcaggaa tcactggaac accttgggtt agaagaaata gcggatcaat
   125221 cgattgacac tctttcaggc ggtcaactcc aacgtgcgta tattgcaatg gtgctagcgc
   125281 aagacacgga ttatatttta ctggatgaac ctttgaacaa tctagatatg aattttgcgg
   125341 ttcaaatcat gcagatttta aaaaatttag tacaagaaat cgggaaaaca attgttattg
   125401 ttcttcatga tatcaacttt gctgcaagtt atgcagacga aattgttgca atgaaagatg
   125461 gacagttatt tacgcaagga acgacccaag aaatgataaa aaaagatgta ttggatgaat
   125521 tatacgaaat ggacatacgg atttgtgaga ttgaaggccg cagattctgt ctttatttct
   125581 aaaactaaca ttaggagaga agagatgaaa aaattagtag taggtatctt gattctagca
   125641 tcatttggtt tagcagcatg tggaaatagc aacaacacca gtcaggcaga cacaaaaaaa
   125701 agcagcacac aaactgaaac tacattgacg ataacagata gtaatggaga ccaaatcgag
   125761 gtgccaaata atccagaaaa agtcgtcgta ttcgacaatg gttctttaga tacgatggat
   125821 gctcttggtg ttggagataa ggtagttgga gcagctacaa gcagccttcc tgagtatctt
   125881 tcatcctata aaaaagtaga atcagcaggt ggaataaaag aaccagactt agaaaaaatt
   125941 aatcagcttc agccagatct aatcatcatt tctggccgac aaagagattt tcaaaaagat
   126001 ctaagtcaaa ttgctccgac cattttcctt tctttagatg cgaaaaatcc atgggaatct
   126061 ttccagcaaa atgtcactgc tttaggggag atctttggta agcaagaaga agctaaaact
   126121 caattagagg aattatctag tgcaatcgat cagacgaaaa agaaagcaga agctactgac
   126181 aaaaaagcac tagtcacact tgtgaatgaa gggcaattat cagcctacgg atctggttct
   126241 agattcggtt tcatccatga tttatttggc tttgaacaag cagatgatca aatcgaagca
   126301 tccactcacg gtcaaagtgt ttcttatgaa tatgtgctgg aaaaaaatcc ggatatctta
   126361 tttgtagtag acagaacaaa agctattggt ggagacgaca gcaaagatga tatttctgcc
   126421 aatgaactgg tagcacaaac gaatgctggt aaaaatcagc agatcatctc ccttgaacca
   126481 gatgtctggt atttaagcgg gggcggttta gaatcaatga aactgatgat cgaagatgtg
   126541 aatcaagcat ttaaatagca taaataacag aggattaaac tctcatacta tgtaacaata
   126601 atgtttctta cattcttaaa tagcaagagc ctatcctcaa agaagttatc aaaaaccatc
   126661 cgtaaaatcg tattgatgat tttacggatg gtttttattt atttgagaaa tcttttttca
   126721 acgtgaattc attatctaca tcatacgggt tgattctaga atggggagtg gcaaaaaata
   126781 gtgtgatcca ttttttcagc atcaagatcg cctcctttga tttgaatcaa aggtacaaag
   126841 cgaaacttag ataaaactta aatttagata tttttatttt gtaccgatct cttctttaaa
   126901 cgatgttcca tctgctgctt ctcggcaata agtccactag actagaaaat atgagaagct
   126961 attttctgtc cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa
   127021 acgatgttcc atctgctgct tctcggcaat aagcccacta gactagaaaa tatgagaagc
   127081 tattttctgt ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta
   127141 aacaatgttc aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta
   127201 tttaggcatg tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat
   127261 cggcgggaag cttactccac cgttttaata attgatagaa ataactctct tctgctttga
   127321 ttttatactc gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat
   127381 aaatatccaa tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag
   127441 aaccctcgat attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag
   127501 tgattcctcc aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa
   127561 tccgtgaaca aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag
   127621 tattttttgt tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc
   127681 tttttccgtt caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga
   127741 aatcataata aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa
   127801 ttagttgcat ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt
   127861 agaagtgaca aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact
   127921 tttattagat atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt
   127981 ggatacggca tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta
   128041 ttctgtagtg gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta
   128101 catggatgat ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg
   128161 accaagcggc ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat
   128221 gggaaaattg gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga
   128281 gctgatgcag ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg
   128341 agatacggct aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg
   128401 attatctacg acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat
   128461 gatggaccat tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca
   128521 gaccgttctt ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta
   128581 cgaggcaatc gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa
   128641 aacagatctc gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct
   128701 ggaagcaaaa aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat
   128761 catgtatcaa tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga
   128821 atcattcgga gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac
   128881 gcatcgtcgc tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg
   128941 ttattattat gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag
   129001 ctatgatcct atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta
   129061 tcaaacacaa catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga
   129121 cagcattagt aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt
   129181 agatccattt atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt
   129241 tgatcggatg atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac
   129301 gataatcaca aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga
   129361 agtctaggca gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa
   129421 ggctatgaca gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat
   129481 caaatcgggc tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg
   129541 ttcttttatt tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg
   129601 atatgtttag taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca
   129661 gcagcaataa tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta
   129721 tacccaaagg aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta
   129781 actcctcctg ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct
   129841 ccaagccctg gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg
   129901 actcaaaaaa tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat
   129961 gggtctagaa aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg
   130021 aaattaagtg tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga
   130081 gcacagctga aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg
   130141 caaacggtga attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat
   130201 gctaaatcag gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa
   130261 aatcaagatg cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat
   130321 tccactaaat tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac
   130381 tgttaaaaat aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa
   130441 ggctaggcta aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga
   130501 aaagatatat tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat
   130561 atctttccga tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga
   130621 gacgaagaaa gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa
   130681 gaaagcagtg aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct
   130741 gagggttcgg acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac
   130801 gatcccaata ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc
   130861 atattggaaa atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct
   130921 atttggtcaa ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac
   130981 ttaggaaatc aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct
   131041 agaatggcta gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat
   131101 tctactcgtt ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat
   131161 aaaaatagtg gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat
   131221 ggacatgtcg cttttgtcac gcctaaacca aataataata attattctgg agagcatact
   131281 ggcgtgacgg tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa
   131341 tgggacgcgg agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg
   131401 attatacctg atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct
   131461 tcaacaggta ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc
   131521 acatcaacag ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga
   131581 ggaaacgata agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat
   131641 aagaaaatgc agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg
   131701 atcaatggaa atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta
   131761 aatctgacag attattttat tgctggagat acgttaataa tcgaattgga gacaactttg
   131821 caggaagtaa ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat
   131881 ttgattgagc aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc
   131941 catgtttatt ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact
   132001 gggaaaatag gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac
   132061 tcggattcag cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat
   132121 tacagaaaag gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat
   132181 aaaatatcca gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata
   132241 gatgaacggg cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat
   132301 ggagattttg agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata
   132361 gggacatccg ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct
   132421 cttttagatt ctacagctca tcgtggaatc agaatagata ttcctgtaga aaaccaaaga
   132481 atcggaacct ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag
   132541 gaagagaaga atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct
   132601 tgtgtcccac agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga
   132661 ttttcttgag accaaaacaa caattattta taacactcct agttatttta ggggcacttt
   132721 ttaacaatat atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag
   132781 agaatcaaat cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag
   132841 tagaagtaaa agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg
   132901 tgaatagcgc aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa
   132961 aagacatgag cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc
   133021 taaaacctaa aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg
   133081 atggtgtatt ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag
   133141 aaactaagga tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga
   133201 tgcaacagac actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc
   133261 aaatcaatgc aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca
   133321 atcaagtagt tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag
   133381 aggaaaaaga aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa
   133441 atggtcaacc gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa
   133501 atgaaaatgg aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat
   133561 gggtatttga aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg
   133621 atgttacgat taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat
   133681 tgtttatttt attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt
   133741 aaggttaaga taacaaatag taaattttta ttattgtagg atggttgggc tctttttata
   133801 acaatataaa atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta
   133861 ataacaggtt tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag
   133921 acaggacgat gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa
   133981 atcagtttta tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct
   134041 gatgactaat gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac
   134101 ttaaatattt agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt
   134161 tgtcgctatc gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa
   134221 agttcgatct agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa
   134281 ttaatggaaa atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta
   134341 tgaaaatggt attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac
   134401 agcatcacgt gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa
   134461 aaaaatatca aatttttttt gaaaagagaa agttga
//
LOCUS       DANJMA010000002       115586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000002 DANJMA010000000
VERSION     DANJMA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(153..1631)
                     /locus_tag="PWU31_000135"
     CDS             complement(153..1631)
                     /locus_tag="PWU31_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082715.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1929..2864)
                     /gene="murB"
                     /locus_tag="PWU31_000136"
     CDS             complement(1929..2864)
                     /gene="murB"
                     /locus_tag="PWU31_000136"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL1082716.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2937..3857)
                     /locus_tag="PWU31_000137"
     CDS             complement(2937..3857)
                     /locus_tag="PWU31_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1082717.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3970..4722
                     /gene="xth"
                     /locus_tag="PWU31_000138"
     CDS             3970..4722
                     /gene="xth"
                     /locus_tag="PWU31_000138"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL1082718.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5586..6119
                     /locus_tag="PWU31_000139"
     CDS             5586..6119
                     /locus_tag="PWU31_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL1082719.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6609..7133)
                     /locus_tag="PWU31_000140"
     CDS             complement(6609..7133)
                     /locus_tag="PWU31_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1082720.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7126..7599)
                     /gene="tsaE"
                     /locus_tag="PWU31_000141"
     CDS             complement(7126..7599)
                     /gene="tsaE"
                     /locus_tag="PWU31_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL1082721.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7722..8702)
                     /gene="pta"
                     /locus_tag="PWU31_000142"
     CDS             complement(7722..8702)
                     /gene="pta"
                     /locus_tag="PWU31_000142"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL1082722.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8855..9535)
                     /locus_tag="PWU31_000143"
     CDS             complement(8855..9535)
                     /locus_tag="PWU31_000143"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1082723.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9661..10530
                     /locus_tag="PWU31_000144"
     CDS             9661..10530
                     /locus_tag="PWU31_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1082724.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10556..11071
                     /locus_tag="PWU31_000145"
     CDS             10556..11071
                     /locus_tag="PWU31_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082725.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11118..11711)
                     /locus_tag="PWU31_000146"
     CDS             complement(11118..11711)
                     /locus_tag="PWU31_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1082726.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11736..11915)
                     /locus_tag="PWU31_000147"
     CDS             complement(11736..11915)
                     /locus_tag="PWU31_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082727.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11992..12501)
                     /locus_tag="PWU31_000148"
     CDS             complement(11992..12501)
                     /locus_tag="PWU31_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082728.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12597..12977)
                     /locus_tag="PWU31_000149"
     CDS             complement(12597..12977)
                     /locus_tag="PWU31_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1082729.1"
                     /translation="MNLTTIHPIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13307..14056
                     /locus_tag="PWU31_000150"
     CDS             13307..14056
                     /locus_tag="PWU31_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL1082730.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14240..15517)
                     /locus_tag="PWU31_000151"
     CDS             complement(14240..15517)
                     /locus_tag="PWU31_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082731.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15596..16240)
                     /gene="pcp"
                     /locus_tag="PWU31_000152"
     CDS             complement(15596..16240)
                     /gene="pcp"
                     /locus_tag="PWU31_000152"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL1082732.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16439..17383)
                     /locus_tag="PWU31_000153"
     CDS             complement(16439..17383)
                     /locus_tag="PWU31_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL1082733.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17385..18071)
                     /locus_tag="PWU31_000154"
     CDS             complement(17385..18071)
                     /locus_tag="PWU31_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL1082734.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18544..18864
                     /locus_tag="PWU31_000155"
     CDS             18544..18864
                     /locus_tag="PWU31_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL1082735.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            19025..19930
                     /locus_tag="PWU31_000156"
     CDS             19025..19930
                     /locus_tag="PWU31_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082736.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19927..20700
                     /locus_tag="PWU31_000157"
     CDS             19927..20700
                     /locus_tag="PWU31_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1082737.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(20877..22301)
                     /locus_tag="PWU31_000158"
     CDS             complement(20877..22301)
                     /locus_tag="PWU31_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1082738.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22512..22958
                     /gene="nrdI"
                     /locus_tag="PWU31_000159"
     CDS             22512..22958
                     /gene="nrdI"
                     /locus_tag="PWU31_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL1082739.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(23028..25010)
                     /locus_tag="PWU31_000160"
     CDS             complement(23028..25010)
                     /locus_tag="PWU31_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1082740.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(25024..25920)
                     /gene="murQ"
                     /locus_tag="PWU31_000161"
     CDS             complement(25024..25920)
                     /gene="murQ"
                     /locus_tag="PWU31_000161"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1082741.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(25923..26984)
                     /locus_tag="PWU31_000162"
     CDS             complement(25923..26984)
                     /locus_tag="PWU31_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1082742.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27196..27762)
                     /locus_tag="PWU31_000163"
     CDS             complement(27196..27762)
                     /locus_tag="PWU31_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1082743.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            complement(27790..29292)
                     /gene="eat(A)"
                     /locus_tag="PWU31_000164"
     CDS             complement(27790..29292)
                     /gene="eat(A)"
                     /locus_tag="PWU31_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL1082744.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(29550..30671)
                     /locus_tag="PWU31_000165"
     CDS             complement(29550..30671)
                     /locus_tag="PWU31_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1082745.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            30797..31738
                     /locus_tag="PWU31_000166"
     CDS             30797..31738
                     /locus_tag="PWU31_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1082746.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31881..32777)
                     /locus_tag="PWU31_000167"
     CDS             complement(31881..32777)
                     /locus_tag="PWU31_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL1082747.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            33059..34399
                     /locus_tag="PWU31_000168"
     CDS             33059..34399
                     /locus_tag="PWU31_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1082748.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34594..35037
                     /locus_tag="PWU31_000169"
     CDS             34594..35037
                     /locus_tag="PWU31_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL1082749.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35299..36717)
                     /gene="nhaC"
                     /locus_tag="PWU31_000170"
     CDS             complement(35299..36717)
                     /gene="nhaC"
                     /locus_tag="PWU31_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1082750.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37121..38344)
                     /locus_tag="PWU31_000171"
     CDS             complement(37121..38344)
                     /locus_tag="PWU31_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL1082751.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38376..39215)
                     /locus_tag="PWU31_000172"
     CDS             complement(38376..39215)
                     /locus_tag="PWU31_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1082752.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39232..39912)
                     /locus_tag="PWU31_000173"
     CDS             complement(39232..39912)
                     /locus_tag="PWU31_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082753.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(39914..40987)
                     /locus_tag="PWU31_000174"
     CDS             complement(39914..40987)
                     /locus_tag="PWU31_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082754.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(41046..41284)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(41327..41560)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41637..42308)
                     /locus_tag="PWU31_000175"
     CDS             complement(41637..42308)
                     /locus_tag="PWU31_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1082755.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(42386..45064)
                     /gene="mgtA"
                     /locus_tag="PWU31_000176"
     CDS             complement(42386..45064)
                     /gene="mgtA"
                     /locus_tag="PWU31_000176"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL1082756.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            45724..46161
                     /gene="ndk"
                     /locus_tag="PWU31_000177"
     CDS             45724..46161
                     /gene="ndk"
                     /locus_tag="PWU31_000177"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL1082757.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(46183..47049)
                     /locus_tag="PWU31_000178"
     CDS             complement(46183..47049)
                     /locus_tag="PWU31_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082758.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(47283..49361)
                     /locus_tag="PWU31_000179"
     CDS             complement(47283..49361)
                     /locus_tag="PWU31_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1082759.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            49854..51071
                     /locus_tag="PWU31_000180"
     CDS             49854..51071
                     /locus_tag="PWU31_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1082760.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(51530..52153)
                     /locus_tag="PWU31_000181"
     CDS             complement(51530..52153)
                     /locus_tag="PWU31_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082761.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(52309..53706)
                     /locus_tag="PWU31_000182"
     CDS             complement(52309..53706)
                     /locus_tag="PWU31_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1082762.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(53728..54699)
                     /locus_tag="PWU31_000183"
     CDS             complement(53728..54699)
                     /locus_tag="PWU31_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1082763.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(54706..57006)
                     /locus_tag="PWU31_000184"
     CDS             complement(54706..57006)
                     /locus_tag="PWU31_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HDL1082764.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(57008..58333)
                     /locus_tag="PWU31_000185"
     CDS             complement(57008..58333)
                     /locus_tag="PWU31_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1082765.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            58564..58989
                     /locus_tag="PWU31_000186"
     CDS             58564..58989
                     /locus_tag="PWU31_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082766.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(59487..60188)
                     /locus_tag="PWU31_000187"
     CDS             complement(59487..60188)
                     /locus_tag="PWU31_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1082767.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(60204..60644)
                     /locus_tag="PWU31_000188"
     CDS             complement(60204..60644)
                     /locus_tag="PWU31_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HDL1082768.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(60644..61219)
                     /locus_tag="PWU31_000189"
     CDS             complement(60644..61219)
                     /locus_tag="PWU31_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL1082769.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(61630..62109)
                     /locus_tag="PWU31_000190"
     CDS             complement(61630..62109)
                     /locus_tag="PWU31_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDL1082770.1"
                     /translation="MFLDSGVVLKETELERIEYTDFGLGAIETEGLNLFVYENNERYC
                     GKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKLSCIIPKGQE
                     RYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDSFTNPAIYR"
     gene            complement(62147..62626)
                     /locus_tag="PWU31_000191"
                     /pseudo
     CDS             complement(62147..62626)
                     /locus_tag="PWU31_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(62798..63499)
                     /locus_tag="PWU31_000192"
     CDS             complement(62798..63499)
                     /locus_tag="PWU31_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1082771.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(63607..65049)
                     /locus_tag="PWU31_000193"
     CDS             complement(63607..65049)
                     /locus_tag="PWU31_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1082772.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(65063..66928)
                     /locus_tag="PWU31_000194"
     CDS             complement(65063..66928)
                     /locus_tag="PWU31_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1082773.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(67049..67900)
                     /locus_tag="PWU31_000195"
     CDS             complement(67049..67900)
                     /locus_tag="PWU31_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1082774.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(68210..68923)
                     /locus_tag="PWU31_000196"
     CDS             complement(68210..68923)
                     /locus_tag="PWU31_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL1082775.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(68991..69317)
                     /locus_tag="PWU31_000197"
     CDS             complement(68991..69317)
                     /locus_tag="PWU31_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL1082776.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(69440..69667)
                     /locus_tag="PWU31_000198"
     CDS             complement(69440..69667)
                     /locus_tag="PWU31_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1082777.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(69989..71242)
                     /locus_tag="PWU31_000199"
     CDS             complement(69989..71242)
                     /locus_tag="PWU31_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1082778.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            71303..72043
                     /locus_tag="PWU31_000200"
     CDS             71303..72043
                     /locus_tag="PWU31_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1082779.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(72129..72887)
                     /locus_tag="PWU31_000201"
     CDS             complement(72129..72887)
                     /locus_tag="PWU31_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1082780.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(72884..73912)
                     /locus_tag="PWU31_000202"
                     /pseudo
     CDS             complement(72884..73912)
                     /locus_tag="PWU31_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(73932..77099)
                     /locus_tag="PWU31_000203"
     CDS             complement(73932..77099)
                     /locus_tag="PWU31_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082781.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSKYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(77323..78207)
                     /locus_tag="PWU31_000204"
     CDS             complement(77323..78207)
                     /locus_tag="PWU31_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL1082782.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(78302..78739)
                     /locus_tag="PWU31_000205"
     CDS             complement(78302..78739)
                     /locus_tag="PWU31_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082783.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(78956..79867)
                     /locus_tag="PWU31_000206"
     CDS             complement(78956..79867)
                     /locus_tag="PWU31_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1082784.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(80324..80752)
                     /locus_tag="PWU31_000207"
     CDS             complement(80324..80752)
                     /locus_tag="PWU31_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082785.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(80780..81418)
                     /locus_tag="PWU31_000208"
     CDS             complement(80780..81418)
                     /locus_tag="PWU31_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082786.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(81422..83404)
                     /locus_tag="PWU31_000209"
     CDS             complement(81422..83404)
                     /locus_tag="PWU31_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL1082787.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            83584..84249
                     /locus_tag="PWU31_000210"
     CDS             83584..84249
                     /locus_tag="PWU31_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082788.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(85689..90086)
                     /locus_tag="PWU31_000211"
     CDS             complement(85689..90086)
                     /locus_tag="PWU31_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HDL1082789.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
     gene            complement(90064..90831)
                     /locus_tag="PWU31_000212"
     CDS             complement(90064..90831)
                     /locus_tag="PWU31_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HDL1082790.1"
                     /translation="MVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGIKEACEE
                     LQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGVELLEDN
                     AQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKRTPDDNP
                     IIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLDLFSFDD
                     EVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            complement(92605..93381)
                     /locus_tag="PWU31_000213"
     CDS             complement(92605..93381)
                     /locus_tag="PWU31_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082791.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            complement(93582..93908)
                     /locus_tag="PWU31_000214"
     CDS             complement(93582..93908)
                     /locus_tag="PWU31_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082792.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(93954..94316)
                     /locus_tag="PWU31_000215"
     CDS             complement(93954..94316)
                     /locus_tag="PWU31_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL1082793.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWVSMVVSLAFIPVVFGAVAVIMGYLYRETDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(94353..94568)
                     /locus_tag="PWU31_000216"
     CDS             complement(94353..94568)
                     /locus_tag="PWU31_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1082794.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            94695..95030
                     /locus_tag="PWU31_000217"
     CDS             94695..95030
                     /locus_tag="PWU31_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1082795.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(95106..97103)
                     /locus_tag="PWU31_000218"
     CDS             complement(95106..97103)
                     /locus_tag="PWU31_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082796.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(97201..97476)
                     /locus_tag="PWU31_000219"
     CDS             complement(97201..97476)
                     /locus_tag="PWU31_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082797.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            97711..98445
                     /locus_tag="PWU31_000220"
     CDS             97711..98445
                     /locus_tag="PWU31_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1082798.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQVEDMNYPEEAVNEIIDN
                     INKLKQTISSIGKKYTKKKMKGGDTI"
     gene            98484..99626
                     /locus_tag="PWU31_000221"
     CDS             98484..99626
                     /locus_tag="PWU31_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1082799.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(99721..101112)
                     /gene="sufB"
                     /locus_tag="PWU31_000222"
     CDS             complement(99721..101112)
                     /gene="sufB"
                     /locus_tag="PWU31_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL1082800.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(101117..101587)
                     /locus_tag="PWU31_000223"
     CDS             complement(101117..101587)
                     /locus_tag="PWU31_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL1082801.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(101574..102809)
                     /locus_tag="PWU31_000224"
     CDS             complement(101574..102809)
                     /locus_tag="PWU31_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1082802.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            complement(102806..104092)
                     /gene="sufD"
                     /locus_tag="PWU31_000225"
     CDS             complement(102806..104092)
                     /gene="sufD"
                     /locus_tag="PWU31_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL1082803.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(104105..104878)
                     /gene="sufC"
                     /locus_tag="PWU31_000226"
     CDS             complement(104105..104878)
                     /gene="sufC"
                     /locus_tag="PWU31_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL1082804.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(105075..105923)
                     /locus_tag="PWU31_000227"
     CDS             complement(105075..105923)
                     /locus_tag="PWU31_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1082805.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(105947..106633)
                     /locus_tag="PWU31_000228"
     CDS             complement(105947..106633)
                     /locus_tag="PWU31_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082806.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(106626..107660)
                     /locus_tag="PWU31_000229"
     CDS             complement(106626..107660)
                     /locus_tag="PWU31_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082807.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(107723..107979)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(108055..108393)
                     /locus_tag="PWU31_000230"
     CDS             complement(108055..108393)
                     /locus_tag="PWU31_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL1082808.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(108395..108751)
                     /locus_tag="PWU31_000231"
     CDS             complement(108395..108751)
                     /locus_tag="PWU31_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL1082809.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(108757..109944)
                     /locus_tag="PWU31_000232"
     CDS             complement(108757..109944)
                     /locus_tag="PWU31_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1082810.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(110135..110980)
                     /locus_tag="PWU31_000233"
     CDS             complement(110135..110980)
                     /locus_tag="PWU31_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1082811.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            111214..112179
                     /locus_tag="PWU31_000234"
     CDS             111214..112179
                     /locus_tag="PWU31_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL1082812.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            112679..113722
                     /locus_tag="PWU31_000235"
     CDS             112679..113722
                     /locus_tag="PWU31_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL1082813.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLFYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(113784..114248)
                     /locus_tag="PWU31_000236"
     CDS             complement(113784..114248)
                     /locus_tag="PWU31_000236"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL1082814.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            114494..115441
                     /locus_tag="PWU31_000237"
     CDS             114494..115441
                     /locus_tag="PWU31_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1082815.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT        33586 a        24067 c        19696 g        38237 t
ORIGIN      
        1 tgtttcaaga aaatcggaat aataacttat gtacttttta attttgttaa gtaatatatc
       61 tagttatatc tagttatatc tagttacctt ttctttgaaa aaaacataaa tcagctaata
      121 aatatgtgag acaaagctgc catccataaa tactagctga ttttttcagt taaatgtcta
      181 tttttttctc tgatgatggt atttccaaaa tattcccagt catctggagt cggtggattt
      241 ttccaaatta tctgttcttt attaaatttt tcaagtaaac aatcagataa ataaatattc
      301 gtacgagaag tgattcgact ctcaatcaca atattgtaat ttttaaatcc tttcacttga
      361 gaaataatgt aatcggtata agcagatcct tgcgaaaaat caatacagac atgaatcggt
      421 gcttcaaaat atgataccgg acaaataccg accaataaaa agagataatc ataaaataag
      481 cgaatcatga aacgttcatc ttttcccaag aaatagcgtt tattctcttg tatgaccttt
      541 tggattgctg tactgaatat tggatacgtc tcgaagaaaa actgtagctg ctttttagac
      601 gtaaagctag tcatctcaaa gtcgaatatc caattttttc gattgacatg catcataccc
      661 atacgaagtt cttttcgtat atatagtctt tcttcttcag agatttcaat cgaaaatggt
      721 aattgatcaa tcaattccac tgacattttt tgactaatca tgtggatatc atgaaaatga
      781 gcttccgcaa gtccaaatgc gccatctaat tcaaattctt cttccgtgat caaaaacaac
      841 atgatataag aaatctcatc aatgatctgc tctttaggga tatgaagaaa agaaatatgt
      901 gataaatcct tgatgatctt cattccttct gagctcttaa agtattcatc tgaaaagaaa
      961 cgttttccga ttggttgatt cccataggcc ctcaacaaac taatagtggt tagcaaattg
     1021 aatttgattc gttttgttgc agacagtttt aaattgaaca gatattcaaa atattgcacg
     1081 atactctttg cttcattcat gacgctttct ctaaatggca tagaaaaacc attaaagacc
     1141 ccgaaatata ccccaaataa tacgttccgt atgtttattt cttccccaac taatatgttt
     1201 ttctttaact ttatttgatg ctgttcaaga aaagagacta attctcttct ttttacatag
     1261 gctcttgata tgcttaaaaa atgattctct gcaaattttt cgatcgttac ttttgaatcc
     1321 acgatctccg ttaacagtcg agcaattttt gatcgttgaa agtagattct tcttaaattt
     1381 ttgatatccg tcaaattcag acgttcgaca tggatctctg cttcttctga aatttggatc
     1441 tctggctttg ttttgaattc agtaaattct ttatttaatt ctatgatata atttcttgtt
     1501 ttaaatttag acaaacctaa tgtttcatat aacttaggca gcactacata attctttttc
     1561 tcaaattcta aatatctagc tagcagcagt ttttctctat cttcctcttc tataaggtaa
     1621 tccaacttca tatcctacac ctcaaaaaat gcctcatacc aattacttga tatgcagatg
     1681 aaagttcatc ttttcaccta tactgatttt atgatggatt atgcttgttt tttcatcagt
     1741 tattctcatt ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa tttatgagga
     1801 aatattctat attttttgtt ttattgtgtt aaaatgtaca agatttgcgc catttgcgta
     1861 aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat tttaagttcc
     1921 attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt ttctccaatg
     1981 atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc ttggatgtgc
     2041 gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat gaatccagca
     2101 tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc ttgttggatc
     2161 agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga agggtattct
     2221 aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt gattgcttca
     2281 taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa ttcttggact
     2341 ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa tgttccatct
     2401 tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata cgcacctgca
     2461 ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc aaatccagtc
     2521 aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg
     2581 accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac tacaccgcga
     2641 atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca aggtatatca
     2701 tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg gaatgcaagg
     2761 acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc atcaaacaat
     2821 atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga ttttcctttc
     2881 ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag tcttgttcaa
     2941 agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact tcttacaact
     3001 tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga aaagaaggcc
     3061 tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc aaattctttc
     3121 actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac ttcataagta
     3181 ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa atccatcgtt
     3241 aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa aaattccttt
     3301 gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata cacagctgta
     3361 tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt tgccgctatt
     3421 cgattttttt gcaggtggat caaccgctta tctgggatac gtttcaattc ttctaccata
     3481 ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc agctaattct
     3541 tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata aacatggata
     3601 ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc gtggatcata
     3661 catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg ttggaacccg
     3721 tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt
     3781 gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt ccctacaccg
     3841 attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca tcttacacca
     3901 ttttttacta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt
     3961 gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa
     4021 aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca ggaaacgaaa
     4081 ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg gaattacgcg
     4141 gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt gaatgccact
     4201 tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt ggaatattcc
     4261 gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa acgtttagat
     4321 taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa aaaacaaaaa
     4381 ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt gaaaaactgg
     4441 aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct ttctcgactg
     4501 ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga aggtgtctac
     4561 tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg gatcgattac
     4621 tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca tacggacatt
     4681 atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg tgaaaaagtc
     4741 gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt ttgcataaat
     4801 ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt gtgaaaaagc
     4861 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg
     4921 gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag gttgtgaaaa
     4981 agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt
     5041 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtga
     5101 aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt
     5161 cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tgagaagttg
     5221 tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt tcctcatatt
     5281 ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaagt
     5341 tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa
     5401 ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact ccaaatcaca
     5461 aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat tctatgtttt
     5521 ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa ggaggtttta
     5581 cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct tatagtgctt
     5641 gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc tatacattga
     5701 tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc catcaagaag
     5761 acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca tattttcaaa
     5821 aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta gcgggatgct
     5881 tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg aaaagtagcc
     5941 gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca
     6001 tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc
     6061 tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact ataaagtaaa
     6121 agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag
     6181 gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt gaccgaggag
     6241 ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc atcaagtaat
     6301 aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact tattaccgca
     6361 gatgcaagct tttggacacc gtttaccaag aggttgtgaa atcagcgttt tgacctgagt
     6421 attagagaaa caaacggcaa aatagttcct catattttgc cgttttttga gctaataccg
     6481 aaggtcagct gattgaacac cgtttattca aaaaaaatgg actgcgacac gacttttgtc
     6541 acagtccctt cgtgtgtttt tctgccttct caaaaggatg acagtttctc actcttttcc
     6601 tatttcttct attcaatcaa ataactcatt ttatatacat ctaaaaattc acctagatct
     6661 gttcttgccc ctctaggcat gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt
     6721 acggctcttt cattacgaac ttgaacgtca agctccaatc gaaaaagtac acccatttcc
     6781 ttcgcccaac tgatgatttc ttctagcata agagtgccta gtcccattcc ccaatattcc
     6841 tgcagaatac tgataccgac ttctcccacg tgagacaaac gaaattgact gtctgccttg
     6901 acagatgctg ttccaataat cgtcccttca tagattgcaa gcagcaataa attattattg
     6961 ctttcgtata tataatccaa ctcttcttct aaggtttctg gtggtaacag gagttctgct
     7021 tcatccaaaa ctaaaaattc tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct
     7081 tttgcatctc ctggcactgc ttcccgcaag atgatcccaa cttcttcact catgattttc
     7141 agcccatttt tctagaattc gctgtttgaa ggtttcactt tgctccccgt atgagattag
     7201 tttcacatac cgcttttcta aatctgtatc acttttttct aaaatcagtt ccaaataatc
     7261 ctccggcagt gcttcttcta atagatttcc ccattcaaca acggaaagcc catcaccttc
     7321 aaaatattca tctaacccta aatctgctcc gctagctgct acccgataaa tatccatatg
     7381 atacaacgga atccgacctt gactatattc tctaataatc gtgtatgtcg gacttttgat
     7441 catttgcgtt atccctaatc cttgggcaat tcctttcgtc aacgttgttt tacccgcacc
     7501 tagatcacca gtcaatatca gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc
     7561 gaattgttct gtcattgtta atcctgatag ttcgatcatt tttcatcttc tcccgctctt
     7621 ctttcttttt gccagtatat cgcgtaatga agttttttta aagaaaaaat gaactagtca
     7681 agaacagcag ctgctgtttt tggctagttc gcgtgtattt gttaatcaat caatgtttgt
     7741 gctgctgtga taatcgataa tttatataca tcttcttcat ttgctccacg agacaaatca
     7801 gaaaccggtt tgttcaagcc ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc
     7861 tgagcaattt tatagccgat atttcctgac tgcaattctg gaaatacaaa gactgttgct
     7921 tgaccagcta cttctgaatc tggcgctttc agttgtgcaa ctgaaggaac ataagaagca
     7981 tcaaattgca attcgccgtc gatcatgtat tgtggcgcta gttttttagc gattttagtt
     8041 gcttcaacta ctttgtctac ttctggcgat ttcgctgagc cttttgtcga gaagctcatc
     8101 attgctactt ttggttcgat atcgaacaat tctgctgttt ttgcagaatc tacggcgatt
     8161 tcagccaatt cttgcgcagt tggattcacg ttgatcgcac aatccgaaaa gacatatttt
     8221 tcttgatctc ttccacgtac catgatcatc gctccgcttg tccgactgat gccaggcttt
     8281 gttttgatga tctgaagtgc tgggcgaaca gtgtcgccag tcgaatggat cgctccagag
     8341 accattccgt ctgcaatccc catatacgta agcatcgtac caaagtaatt cacatctttt
     8401 aaaatctttt ctgcttgttc tttggatgcc tttccatttc tacgttcaac aaaagtatcc
     8461 accatttcat cccacttatc gtaattatca gggctgatga tcgtaaagct agatgttttg
     8521 attcctctgg catgcgctgc atttcttatt tcttcttcgt tccctactaa aatgggttcc
     8581 acaagttctt ctgctttcaa ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc
     8641 gggaaaacga ttttgatccc acggcgaaca attttaaatc ttaaactatc aaacaattcc
     8701 acaagatgac ctccagacgt ttttatatgt attatcatac actattccag aaaaaaagaa
     8761 cagtttattt tatctgtatc agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa
     8821 gcgaactgca ctataacagg aaagcgctgt tttattacac tgtttcaggc agttgccaat
     8881 caatcggttc ttctcctaac gcaaggagcg catcattcgt tttcgagaag ggtttggaac
     8941 caaaaaagcc acgacttgca gataacggac tgggatgtgg agatgttatg atgatatggc
     9001 gactcttatc gatcattttg attttttctt gtgccggttt tccccacaag ataaacacga
     9061 ttggtttttc ccgttcattt aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt
     9121 tcccgcgatg agaatacgct tgcccttccc gaacggtaag aacggtgttg agtaaaagga
     9181 ctccctgttt agcccatgac actagatttc catgttgaac aggtgcgatt ccaagatcat
     9241 cataaagctc tttatagata tttcttaacg atggcgggat ctttacgcca ggttgcacag
     9301 aaaaagataa gccatgagct tgattttctc catgataagg atcttgtcct aaaatcacca
     9361 ctttaacttc ttcataagga gtcaattcca atgcttcata gatatgatac atatctggat
     9421 ggatcttttg ggacgcatat tcttttttta aaaattctct tagatgcaga taataatctt
     9481 ttgaaaattc tgaggacaaa acttcttgcc aactgttatg aatgatttct ttcaaaaact
     9541 ccacctccta ataaaaagaa taactcacat tttcttcaat cacaagggac aatcttcacc
     9601 ctcagcactt gattaatatg gtaaactagg aacaagaagc agaaaggaac gataatcatt
     9661 atgatcaaat taattgcatc agatatggac ggcacattat tagatgccca tatgagtgtt
     9721 tctcctgaaa atgccgaggc gatacgtttt gcaaatgatg ccggtgttga gttcatggtc
     9781 gctactggtc gaaactatca agaagctcgc gcagctttag atgaggtagg aatcgattgt
     9841 gcgatgatca ctttgaacgg ggcacaggta tttgataaag aaggaaattc attgtttacc
     9901 gtgtcgattc caaataatca agcaatctct gttttagata ttctagatgc taacggtatt
     9961 tactatgaag tagcaaccaa cgatggtctt tattctgaaa atcaagcgaa acgaattgaa
    10021 agttttgctt ccatggtggc tacccattta cctcacctta cgtataaaat ggcgattgcc
    10081 atggcctcag ctaagctgga gttattgcac atcacatacg tggacagtat tcgtgctatt
    10141 ttggaagaaa agaatctgga agttttaaaa attatttgtt ttcatacaga agggccagct
    10201 gtattaggtc cagttggaaa gcagatttct cttttaggcg atctagccgt tacttcctct
    10261 ggtcaaaaca atatcgaagt gaaccataaa aacgctcaaa aaggaattgc tgttgcccat
    10321 gtcgctcacg agcgtgggat ttctttagaa gaagtcatga cgattggcga taacttcaat
    10381 gatgtcagca tgctgcaagt agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa
    10441 gtaaaagatt atgccaaata tacaacggac acaaaccttg aatctggcgt aggaaaagcc
    10501 atattgcgcg cgattgcaga agacttgtag ttttatctag aaaagaggga tatagatgtc
    10561 agaatttttc attcaggaaa agcagcttgg acgtgcaact cggacaattg tcaaaaacga
    10621 aaaaggacat tccctgtttt tgatggttgg tcgctgggga acaaagggcg atgcattgtc
    10681 tctttatgcc atgaatggcg atctcgttgc tcacatcaag cagataagcc tgacattcgg
    10741 tacgcgtttt gagctgtata aagagttcga aaaggtggga accatgcaga aaatattcaa
    10801 ttggcccggt gacttttatt atattcgtag acttcactgg acagtccaag gtgatatcta
    10861 taatcatcaa tatcaaatcc atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac
    10921 acttttttca ggagattact atgtattgga tgtctcaaat gatgaagatg ctccagtttg
    10981 tatttgtatt gctgcggttt tagattactg gctctaccgt aaagacaaaa gtcgcgataa
    11041 gcctgtttct taccgactta acttaagtta aaaagatgta cctgaaatgc ggaaatacgc
    11101 atttcaggta catcttttta gatattcttt tgcatatgat cataaagatg accactgatc
    11161 atccgctgac cgacaacttt gaagtcatgg cgttcgtata attttttagc ctttggattt
    11221 ttttcatcca cacttagacc gatgacggag cgattagctt tcttcgccaa tttaggtaat
    11281 gcttctaata atcgcgaacc gatgccttgt cctctaaaat cttctgatac actgatagag
    11341 tccaaatacc attcgttggg aaacgtttct ttatcaatga acaaacgtgt agacgcatct
    11401 agctgatatt tctctaagat aggtaccagt ggctgatcga tgattgattc ttcttcatct
    11461 gtataaccgt aagcaactcc tgcaacttta ccgtctattt cttctacgat acctcgtgaa
    11521 taactgtaac gataatttgg atcagtgatt gcttcttcta aaatctctaa agtcttttgt
    11581 tcccctgtct gcttcaaaaa tggcagttcc atatctttta gaatcactaa gatcaatggg
    11641 gcgatttctg gagcatcttc ttttctcgca aaacgaatca tagtttcctc tccttttctc
    11701 cattctaaca tgcagaaaaa gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt
    11761 tcagtaatga aataaccatg agtaacgatt cttgccgctt cagttcggca ataagggcag
    11821 acgactggca tttctttatc attatgttcg actacattat tgacttcttc gtatgcaaat
    11881 ttttttccgc aattagggca tttcattttt tccatgttat ctcaacttcc tttcatcttt
    11941 actctcttag cttactctta tattgtaaaa aagagaagca atatccttct tctatgcaga
    12001 tatccatacc tgacggtcct gttctccatc tattaatcgg actttataat aaggacgttc
    12061 atctttgact agaagtgtcc actctttcac ttttgctttt ttcccgtagt agctgactgc
    12121 ttccttcatc gctgcttgcg gttgtttgaa ctcttccgtt ttttctatat caaaaagatg
    12181 ttctgcggcc ttttccgtgt catttcgtac ggttgttttc ccagtggatg gattgactgc
    12241 tatgacttgt ccttcacaaa aaaactggta ttcatatgtt gcattcgtag gattctccaa
    12301 atctttttca actgcaaaat gaatagcaga tatgttatgt tcttttgttt cgttttttac
    12361 gattgctgct gcttttttca atgagatccg atattcctgg tcactaatat catccatgtg
    12421 aaaagcggca ttgagcagtc ccgtcatacc ggtaatccct attacgatga gggcagcaac
    12481 accaaacatc atttttttca ttcttcattc tcctgtttct tttttttaaa gaatatcaag
    12541 aaatgaacga atgaaaaatc gaatgaaacg aaaaaaaagt gacaattttg ttatctttat
    12601 tcgtgcagtt ctaatggcaa tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta
    12661 aaggtatctt ctctaatcgg ttctgattcg atccctaagg actgcaattc tgcaattgtt
    12721 tcttcaatat cgtctacttt gaaagcaaga tgacgcaagc cacaagcttc tggatagttt
    12781 ggtcgttgtg gcgcattagc aattccaaaa atctccaatt cagcacttcc tagtttcaga
    12841 tctaatttat agtcatctcg ttctttgcgg taattttctc tgattacttc aaatcctaat
    12901 ttattaacat aaaaatcacg tgatttctga taatctgaaa caatgatggc tataggatgg
    12961 atcgttgtaa ggttcatatg agcactccta ttcttcgttt aagctcggat ctatattaat
    13021 ctccagttcc attgtaaatg tgtcagattg aaaaaaatag ctgtttttcc tttttataag
    13081 aaaaaaggca aaaaaaacag aaagtaggtc ctccaagaca ctcttcctgt tcccaatata
    13141 ataaatgaaa gtaagaaatt atattttcaa gaaaacaaac agaaaattat cataacacaa
    13201 attatttata attgaaagac tagttttttt tcaagaaaag catacaatag attgtgttag
    13261 taaatacaga acacactaca tttagtgtct taaggagtcc tcatacatga agtcatattt
    13321 tcagttttta gctcatcagc taaaaggctg gccgcaacaa aattattatc ttttcttttt
    13381 cagtcttggc tgccaggtca tgactcttgt caatagtccc atcacttggc tgtccgttat
    13441 tacatttatc ggtaccacgc ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc
    13501 agtcaacggt attttaggta ttttgtctgc gatttgtttt attatcgttg ggttttcagc
    13561 aaagaactat ttaagtatcg gtgaacagat tgcttatatg atcacattgg atatccccgt
    13621 attgttaagt aaagaatgga atcataatat ggcttcaaaa atccgaaaat taactggaaa
    13681 aagctggttt atcgcaatca tcagtacttt ccttgtctat cttgtttcag gattcctgat
    13741 tggtcgttat acagatgatc ctcggccgtg ggtagatgcc attgcttttt ctattagctt
    13801 gactgctggt atcatcagtt tcttgcgtta caacaaccaa tattactggt ggttagcttc
    13861 agggcttgct caaatggtcc tttggtatat ttcatttacg catggatctg caagtttggc
    13921 gatgttcgtg aacagctctg tttacttgct gaatgatgtc cttgcattta ccgtttcacc
    13981 ttggtataac caaaaagaac gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc
    14041 acaacaacac gcataaaaaa actgagcgtg ggacaaaagt aaagaacact tttgtctcac
    14101 gctctaaaca cgtataaacg gcggaatcag aagcaactcc ttcggaaata agctgaaatt
    14161 cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt
    14221 ctgtcccagc ctctattttt tatagtaatt ccttcagata ctttcttgcc tcatcattga
    14281 aacgcagtga ttcttctaac ttctgataat attccaacgc ttgtttctga gcttcagtaa
    14341 aacgataatg ttttcctttc catttgtcga ttgtctcttg cacatttcca gattggatat
    14401 ctttcactac tagttgccat ttttcttttg agactgtacg gtctttctta tcagcagatt
    14461 gtatagcttc tcgttcttta cgcaatactt ttaaaaaacc atttctgact atccgaggaa
    14521 tgctgtcgaa gaacccttct gtaaaaccat agcgacaata ttgcttccaa ataaatagat
    14581 agatttttgg ttggatgaat cgttggtttt tcgcaaaata ttccatctga gcagtgactt
    14641 ctggtgttcg ttgacgtttt gtctttctca acacggaaat aaatgtttca aaataagcat
    14701 taggatgtac tggcacttct tcatatacga tggattgcca atctttacgt aaaaatgcgc
    14761 gatagttgat caagaattct tctacatcct ttactaattc ttctgcaaag tcgtcaaata
    14821 gttctttttt tatctgtcgc ttttttgact gcccattgat gtgttcactt attttcttga
    14881 tttcattttc caatgcttga taatccgcta tgtaaatcgg catttctact tggcgaaatt
    14941 ctgcgagccg ctgagctaat tttatatccg gtaaatatgg ctgtttcatc cgacgatttt
    15001 ggtacaagac aaaacagtat ttttgccata gtccttcagg aaaatcgata ttcttctgct
    15061 taagccataa taattcgtct aaggccagat caacttttgt cattccctga tggatcgaag
    15121 ctgccactgt cggagagaca tgcaggtgat cggcaaagtg gttgatgcct agttttagga
    15181 tctttccggt ttttggcgat ttaacgatgt attgcacttt taattctcta ccacactcac
    15241 aatacaattt ttgctggctt cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa
    15301 acacccaatc tgttgcagct aaataatgtt cttccataaa acgagagctg attagatatc
    15361 ggcggtgctt atcgactagt tcccgctgtt ttttcgttaa ttttgcatag atctcccaac
    15421 gttgactgct tgtgtattgt ttagaaattt tgatgggata gcttttgctg atttcttttg
    15481 gaaaccattc ttcaaagacg gattgccatt cttccacggc tgtttctttc ctttctaatt
    15541 acaaaaagct ggctactaac ataacgttag caaccagcca gtgtttcttg ctaaattagt
    15601 gagttgttcc ggcatttccg gaaatatctt ctttattttc tactgctgct tcaactgcat
    15661 aagtcaaaga agaagcaatg tcttctaaag acatagaagg cgtacccact ggacgatcga
    15721 tcacttgatc gggtgagaat ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg
    15781 tttcgatcaa atgaagcaaa cgatacatga tatcattgca gacaaatgtt cctgctgtat
    15841 aagagatgta cgctggtaga ccatgatctt gtacattttt tgtcattgct ttgattggca
    15901 atgaggtaaa gtaagcggct ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc
    15961 cttcattatc agggattctt gcttctgcta gattgattgc gacacgttcg aacgagactg
    16021 ctgaccgccc acctgcttgt ccgacacaga tcacgacatc tggttggtgc tgttggatgg
    16081 cttctttgac tacttgactg cttttttcga atacggtcgg tacttccact ttgatgattt
    16141 cagcaccgct gatttcatct ggcatttttt ttactgcttc ataggctgga ttcactttgt
    16201 ccccaccaaa tggatcaaaa cctgttacta gtactttcat ctgttgttcc tccgtccgat
    16261 tgatttaaca tatggttatc ttttatcata ccattttcct tgaacagcgt aaataaagga
    16321 aacaatagtt taaataacat aacagatcaa acttaatata ttcttataaa aaaatagtgt
    16381 tccactacaa tgttagaaga ggttgcgaca attattctgt cacaacccct ctcttctatt
    16441 atttaaatag aatcatataa gcaatttgga aaactagcat aaataacgca ataaacaact
    16501 ggtttttgat cactccgtat ttgtctttca tctccagcat cgctacaggc acgatattga
    16561 agttagcagc catcggtgtc agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc
    16621 ctattaacgc tggattcgct ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca
    16681 tcacggtgat ggccgcaaat gcattcccca tgattaccgt aaatatcacc atgccaagag
    16741 cgtaaatgat gatgccgatg ttcacatttc cttctggaac gatcgtacct actgcattgg
    16801 aaatcactgt cccaactcca gcgcttgtaa ataccgcacc tagagaagct aaaagcattg
    16861 gcaacatgct taacggccct actgtgccca gcatatctgc tgcatcatct aaaaatgtac
    16921 ttggtcgatt atctcgagaa aagaacatca agattagcat ggctgcaaaa acaccgacgc
    16981 ccacgccgac taaagcacct aaattggtaa atagagcaaa caagatcgca aagacaccga
    17041 tactcaaagc tggaataaaa attttcattc ctagtttgtc tgacattttt tccatatatt
    17101 cttttgatgg aagccggctt tctcctttgc tgactttctt agcaattgct ggtatggtca
    17161 tcgcaaagat caagccaccg ttcacgattg ccggaagcca tcttcctcct gcaataacta
    17221 cccctaagac cgtccagaaa aaggctgacc cataacgatg aggattggtt ttatctgtta
    17281 aatttcttac tccagtgtaa atcaatacga gtcccatcac gatataaagg atctccagta
    17341 atttatcgcc taataaaata tcaggatttg taaaaaagtc catcttattg cccctttcct
    17401 ggataatatt tcttcgctaa tttcttttct ttaagataga agtagacact tgccacaact
    17461 aaagccgtga tagccactgg aacttccact aaagcaagct gtcccaatgt tacgtcatat
    17521 cctagatctt tcagtgttga ttggactagc aacgcccctg ctcctccgat gaatagtact
    17581 tggccaaaga accagcagac attttccata gctgaagcca taccttttag ttcttcttca
    17641 tgcttaggat ggacttctaa cccttttgat tcgatggtcc ccaatgccat cggtaaaatg
    17701 attggacgaa caaatccagc aactccacca aaactcacgt taaaagccgc aaagatcatt
    17761 cgaaagatcc cataaatatc tacaatcata ccagcagata ctttcttgaa tcgcttgatc
    17821 aaggaagccg ctgattcttt cagaccattt ctttcaagcg ttcctgttgc caacataatg
    17881 atgataaaaa tcgccatccc gcgattgttt acaaaacttg tacctaaaag ttctaacatt
    17941 ccttgaatgc caagaccgcc agtcaaagct gtaacaacta aagcaaccat tacgatcaat
    18001 atcgagtcta atttaaatgc aaacccgaga ataacaatca agacgcctaa aaggctaaga
    18061 taattttcca tatattttcc tccataaaat gacaatattt ttttaattat aaagcaaaac
    18121 acataaaaaa tatagttgtt ttctcacgaa atagacacta tttttaaata tttttcgtta
    18181 tttttttatt atataattca ccttacttat tgtaagataa agcttttttt aatttattgc
    18241 ctgctaatat ataaaaataa ggtctagtta ctgttttttt aaatgattaa taaataacaa
    18301 ctcctttcta tgaaattgca aagcattcat cttcttttct gctaacgata tatccgtcaa
    18361 tgcttgttac actttactaa aaagtatgag attgccgata tctggatagg tgtgctatac
    18421 tacagaaaaa gatatacgat ctgccaccgg gtagtaaaaa agaggatgat cattccatta
    18481 ggtctttgaa gggatgattc tcctcttttt gtgtattttt tatattgaag gaggaacgta
    18541 tgtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg gtcaacgatg
    18601 atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt gattggaatg
    18661 attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgcctatgag tctctcttat
    18721 cctatttgga cagggattgg cgcagttggt tcggtcatta ttggtgtggt cttgtttcat
    18781 gataagctga atctttccac ttggttcttt gtcgggttgc tgctcatcag catcattggc
    18841 atcaaaatca ctagcggtca ctagatactt agacaagcag acaaggactg atcagaaaaa
    18901 gcggggcttt ttatttgata ttcctctttc tatgaaaaaa caagaaaata agactaatga
    18961 cctagcgact tctctactca tcaggtgtat gcttgtacta tctatagaaa aggagtgaat
    19021 atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca aacaagccct
    19081 cagcgatgtc agttttagct tatctcctgg caaaatcgtt ggattagtag gtcctaatgg
    19141 cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact cagaaggaac
    19201 gatccgtatt ggtgaaaatg agatttctcc ctctagtcat aaaggattag aacaagtagg
    19261 agcattgatc gaatatccgg gtatctatcc attcttaaca ggttatgatc atctgaagtt
    19321 attctctgaa acaaacgatg tatctgctat cgacactatc gtgaaacagc tcaagatgga
    19381 aaaatacatt cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa aattgggaat
    19441 tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga tgaacggttt
    19501 ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta ctcagggcac
    19561 ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg atgagctgat
    19621 cctgatcaat caaggaaaaa tcatcaaaca atgtacaatg gctgaattga accaaacagc
    19681 gcaagatttc atcgttatct ctacgacaaa tgatcaagct gctcgaacga tcttgacaga
    19741 cgctggatat cccttggaag atcctgataa aatcaagctt ctaaaaaaaa acgaagagtt
    19801 attaggaact gttatccagt tattgtctgc agaaaatgtc catgtgacag atgtccagca
    19861 tctgacgaat gatttggaat cttccctatt ggaaattctg gattcagtaa agggggaaga
    19921 agaaaaatga cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa aaagtcaact
    19981 gctgttttat ctgttctatt agttgctttg ctcataggaa cagcacttct agccaaaaaa
    20041 tacacgacga tcattgatcc agttgaaatg actgctcagc ttttcagtgc cacatcttgg
    20101 attgtcttta tcatgattgc tgctgcaagt acgatcattt caatggaatc acaatatggt
    20161 acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt aagtaaatgg
    20221 atcacattag ttatttattc tatttattta taccttttag ctattattgt caccgtgctg
    20281 atgaaactga tccttttccc ttcgatttcc tttacggaga agatttctac tggtcagaca
    20341 ctgatccaaa gcttattcac ttatactttg ggtagttata tcggtttatg gctaatttta
    20401 agtttagtgt tgatgattgc ttgttttatc aatagttccg gtgcctccat ctcagcaggg
    20461 atcgtctttt attttgcttc atcaatcata tctggtatcc tcatcgcact cattcaaaaa
    20521 tgggagtgga tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca aattggcaac
    20581 gaagagatta tgaagcaatt gactcatctt tctactaatc aaatgctgtt tggcaattta
    20641 gcttacattg ctttgttctt agcattggga tacctcgtat tcaagcgaaa aaatgtatga
    20701 tggttcatta acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt tcggcttatt
    20761 tttagaagaa gctacttctt gaacaccatt tattcaaaaa aagaagcgat gacacgaatg
    20821 ttgtcaccgc ttcttttttg aataaaccac aggcttcaca gcctctcttt atacttctat
    20881 acgtgaagca tcccagtggc aaataaaacc attgcgatta ctccgaccat cgtcacaccg
    20941 ataagtaaaa tcttatctaa gtggatatgt tgtacaggtt cgttttgttc tttacgtgct
    21001 ttgaggtaaa aataaatacc tggtaagaaa agaacaagaa caagcatcac ttcattaaag
    21061 gctacaacta gcatggccca tacttgaaaa gcacttgcta aaaaccctac taaaaattgg
    21121 acccactctt tccgctgata agaaagcttc atttggtaca gccctaagaa aagccacgtg
    21181 aagaaaatcg ctgctgtacc aagtgtataa gcaaagttat atgcactgtc agtgaataat
    21241 aatgaaagta aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc
    21301 ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc cgtcctcagc catattcccc
    21361 attgtttcaa tcggcaagat tgtccatgag agccaaaccc caactaaaga aatgatcaaa
    21421 ccgccgttga tcacgaccgc cccccattta ccaaccatca tttcaaatac atatcccata
    21481 gcaggttgcg ctttgatcgt ttgcagctga ttcatcgtca ttgtgccata tggaagcatg
    21541 gaaaccaaga cgtaaataat gatcaaaccg aatattccta gaatcgttgc tttcccaaca
    21601 tccgattgtt tttttgctcg tgaacctacg acagaagctc cttcaatacc aacgaatacc
    21661 cagagcatca ccatgatgca actcaatact tgactagaaa acgggatcgg ctgcccattc
    21721 gcattcaata tgatatcgct agaaaagtta ttaatgaatt gttggatcga aaaggtgctg
    21781 atagctacta caataaataa aaataatggc actaatttac aaaatgtgac gatagcattg
    21841 acaaagctag cattttcaac tccacgattc actagccatg catatcccca taagaagatc
    21901 gtactcatca cgatcgatgc cacattttga ccaccttcga atacggggaa gaaataacct
    21961 aatgcactca tcaacaatgt tgcgaacgcc acgtttccta accagcatgt gagccagtaa
    22021 ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc
    22081 ccgcctttta aatctggacg ttttttattg atattattga tcgataagga gagcatcaac
    22141 attgctgttc ctacgagtac ccagctgaag atagctgggc caccagccga tccggagatg
    22201 tccgtcataa taccaaatac gccacctcca atcgctgaac caatcactaa ggcagcaagt
    22261 ggaatcaaac caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaagta
    22321 aaaccgcttt tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac
    22381 tttaatagaa atataattct accgactttt ttctcattat acaagcaaag cgcctagtcc
    22441 ctttccaaat tacaccacca aaaaaactta aaaagatgct atactaaagg gaaagaattg
    22501 ggggatgatc catgaacatt ttatatatat cgatttcagg aaatacgcgt gcttttgcca
    22561 aacatctagc agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta
    22621 ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt
    22681 tcgttcctac ttatttagac ggcgggaatg ggttagacaa cggtgacaca gaaatcttga
    22741 ccgaaacgat gcgcgaatat ctggaatacg aagacaatca taaactttgc cttggtgtag
    22801 tcggtagcgg taataaaaac ttcaacaatc aatattgtct gacagccaaa caatacgcac
    22861 aaagattcgg ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga
    22921 cacgcgttta tcaaattttg gcagaaaaaa ataaataaca caaaagaaga agctgaaaaa
    22981 taaatttcgg cttcttcttt ttatgttctc tattgtttct attcttatta agctttgaca
    23041 tccatcacag gtgcttttgc tttcgcttta cctgtttttt ccaagacaaa gttttttaca
    23101 cgatccatat tggttaccaa gacgatcatg gaggtttctt tgtctgctgc tttgattgct
    23161 tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgtccttc tgttactttc
    23221 acatcgaatg gcgtaccgcc taagtcaact gtgttgatgc ccatgtgaag caagatttct
    23281 aagccatttg gtgtcgtaat gccgatagca tgttttgttg ggaaaacaga acttattact
    23341 ccagtaaccg gggaatagat atgtccgttt tctggttcta caaagtaacc ttcacccatc
    23401 attttttgtg caaaaactgg gtcacttgct ttatctaaag aaacaactgt tccgtcagca
    23461 actgatgata agatagtcac actttcagct gaagcttgag gtactgcttt ttcaggtact
    23521 tcatttactg tggctgtttc ggtcgctagt tcttcttttt ctaaacggtc tttaggaata
    23581 ccgaagaaga atgtagcaac gaagccacca gcgtaagcag ccaataaacc aaggatatag
    23641 cctaaccatt taccatcaga gatcaatgga atcaaagcgg ctcctgaagg tccaatagcg
    23701 attgctccga catttccgat cattccgatc actgccccac cgatacctcc accgatacaa
    23761 gcagtaataa atggacgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt
    23821 cctaagattc cgactggtaa agcccctttg atcatttctg ttaattcttt gtcttttttg
    23881 cagcggatcc ataatgccaa tgctgcaccc acttgtcctg ctccggccat cgcaaggatt
    23941 ggaagcaata aggtacgacc agtttcatca atcatttgta catggattgg cgtcaagatc
    24001 tgatgtagtc cgaacatgac cattggcaag aataatgtac ctagaacgaa gccggcaaat
    24061 gctcctccaa caccaagtac ccagttgatc ccaccgacca tgccgtcaga aatgaaccct
    24121 gctaaaggca tgatgaagaa aatttcgatc agaccgatgg caatcaggga aagagttggc
    24181 gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac
    24241 aataaccaaa ctgcgaagat aacacctaag atcccgcctt gtccagttgc aagagcttca
    24301 ccggtaaaca agtttttcag tggtgcttct ggattcatcc ctgtcagcat cacaacagcc
    24361 ccgataactc caccaagcgt tggcgttgca ccaaaaactt gtgcagcatt gatacctgta
    24421 tagataacta gatagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt
    24481 tgccatgttc ctgcgtctag tgttccagct gtgactaagt ttgctaaaac tgcggcaatc
    24541 cctgccacga tcccagtgcc gacaaatgca gggatcattg gtacaaagat attagagatg
    24601 tcttttaata ccgatttgat tttagagggt ttttgtttcg atttttgtgc cgctttcatt
    24661 tcttgtgccc gttcgtttac acggtctttt ccagatgcag aagaacttgc tgtagttgca
    24721 ctagcagaag gaagtttttc tcctaatttc acgcctgcta agtcaaccat ttcctgcgct
    24781 actttgttca ctttcccagg tccgatgatg acttggagct gttcatcctc tactacgcca
    24841 agtactcctg gaatcgcttt taattccgag acatttacaa gatcatcatt gatgaccttc
    24901 atacgtacac gagtcatgca gtggacaatg ctttcaacat tgcccatacc gcctgctcct
    24961 tgataaattt gttttgccag ttcttccaaa gtcaattcgt tttttgccat ggtcttttcc
    25021 ccctcataag gttcctttta caaaaccgtt tgcttcagct aattttgctt gagctgcctc
    25081 tttatcacta tttgtcaaaa tcatgacgat agccagtttg acattttgat ctgcttcctc
    25141 aaagtacttt tcagctgttt cgtagtcagc ttctgtcgct tgcatgatga ttcgttttga
    25201 gcgttcaatt agtttttcat ttgttggttt gacgtcgacc atcaagttat tgaatacttt
    25261 accgataccg atcatactga tagtagaaat catattcaaa atcagttttt gtgctgttcc
    25321 tgacttcaat cgagtagagc ctgtcaaaaa ttctggtcct gcatcgactt cgattgggaa
    25381 atccgcatgc tcgctgattt ctgcttgttt attgcaagaa atcgttcctg taacagcacc
    25441 aatcgatttt gcgtaatcta atccaccaat cacatatggt gtacgaccgc tagctgcgat
    25501 accaataact atatcatttt ttgttaaatt cagcttgatt agatcttctt gccctaattc
    25561 ataagaatct tcggctcctt cgacggctac agtcattgct tcttctccac cagcaattag
    25621 gcctatgacc atttccggtt ctacaccgaa tgtcggtaca cattcagcag catctaatac
    25681 accaagtcta ccactcgttc cagctcccat ataaatcaag cgaccacctt ttttaaagga
    25741 ttcaatcgtg ttggcgatca ctggctcgat acttggcagt tctttttcaa cagcagcggc
    25801 cactttctga tcttctttgt tcatgagttg gactgcttga gaaatactca tttcatctaa
    25861 tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat
    25921 ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc
    25981 agcggtagtt cttctttgat gactttggct actacattca ctttttcatc cgctggcaaa
    26041 tcatatttag ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta
    26101 atagatcctt tcatacgctt agctgtattt cttactggga ttggcggaat ctctcgaaaa
    26161 cgcgcatctg cgcttcgtat gacatctctt gcatcatctt gccgattcgt atgttttcct
    26221 aatatatatt cgtaaaaaac ttcatctaca ggttccgtat ggagcaagac tgtctgttct
    26281 ttttggaaag aagcaaattg ctcctgaacg tgttctgaca gacctgcatc tccgatataa
    26341 acaatatccg tgttacttgc taatagatca agagcacctg ccagaggatg gatccctcga
    26401 tgttcttcca aagttggtaa tccttgatac agtggacctc tcaactctgt atctcctggt
    26461 acaaagcctt gtacaaggaa tccttgagtt ttgagccatt gatttttttc ctgataccat
    26521 tctttgtcca accctgtttc aagacgtgga taataattat gccaagcttc aatctgtgag
    26581 aaatcagccg ctgcttcttt caactcatcg atatcattag gcgtgatcgt gctggcattc
    26641 aaactgattt tcaattgatg tgaccagtcg gcgatctgct gattagaaat atgataatcc
    26701 attcttaacc ctgttacacc aattgctttc aagggtgtta gatcttctaa tgaaaagcca
    26761 gctcgtttca atgcctctcc tgaaatatcg accatcaact ctagtttatt ctttttcgct
    26821 tcgcttccta gttcgatcaa tcgattttga taagccgaaa catcatcttc cggtatatgc
    26881 atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc
    26941 tcttctgaca aagaatcatt cataaatatt gaaaagccaa acatattatc cctctttttc
    27001 tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaatact
    27061 tttgttaaaa taattaaatt ttatttctaa aaattggatt tataatgcca aaagaattga
    27121 cacagccaca aaaaaggcac tgctaagcaa aagcagtgcc ttgaaacatt ttatttaaat
    27181 aacacaaaag tctaattact cataagaaat taattctgta ttgtctacca tgaatgtaaa
    27241 gaacccttca ttaggcattg gatgaaaata agcattgatc actcttatca aggcattatg
    27301 acagactaac aagtagacat tttcttcgtc ttccatgcat tctttcaata aaggaacgat
    27361 ccgtgcgtac acatctaaga ccgactctcc atttgggaaa cgtacagcaa acgctaaacg
    27421 cgctttttga aagttttcat ctttgcttgg cagtccgtca taatccccaa aatccatttc
    27481 taccaatcgc tcatcagcgg tcatcggcaa attgagacga tcaactactg cttgtgctgt
    27541 atctcttgct cgttgaagag gggaatggat gattttcgtg attggaacag gcaaatctac
    27601 taccttttct gctagtgcct gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc
    27661 tcgcccgcaa actaatcctt gtgcgttcca agttgtttct ccgtgtctcg ttacataaag
    27721 catgtgattc tctccttcgt tgacaaagtt ctccttaacc ataccttgtt gtctttgaga
    27781 ttacaaggtt tagattttct caagagaaat aatctcagta gatactttat ctagaaaggt
    27841 ttgatcatgt tccactagta acatagcagg tttcacgttc aaaatcagtt gttctaattg
    27901 ttcttgattg aagacatcca aataattcaa tggttcatcc catatataga gctcagctgg
    27961 ttgggataaa gatttagcca attccacttt tttccgttgt cccatactca tctgttcgat
    28021 tttattatga aaaacatctc tttccatccc aagctttcgg agattattca aaaatgcttg
    28081 atagtctact cggtttttct tcgcgaattc cgccaatgtc cctcgattgt cttcataatt
    28141 ttggcgtacg taactaatgg tcaaatgttt gggctgggat ttttctccta caaccttgcc
    28201 gttgaatgtc cccagaagat aatggataat ggatgacttt cccgcgccgt taggacctga
    28261 aattgccatc cgctgatgag gttcgatcgt aaaatgaact ggctcgaata acagattttc
    28321 ataccctaat tgaagatttt ctacgcttaa cagccgttta tgatgagaca cttggctatt
    28381 catcgtcaaa gcatcgatat actcgatatc ttttaataat ttttctttct cactgatctg
    28441 ggtttccatc cgattcacga ttgctttaga tcgtttcatc gtccgtgcag catcagcgcc
    28501 tactgctcct ttattcactc gcctagattc cgtatcgatg aatccgactt ttttctttgt
    28561 tttgtcccct tctcgggaac gagaccattc tgctttttca gctgctgttt tctttagcct
    28621 gctgacttct tttttcaatt tttcattttt agctatttca aattcatcac gaagtttttt
    28681 ctgttcttca tagatagaga aattcccttg ataaagttcc agttggctct tttcgattgc
    28741 taaaacatgg tccactactt catcgataaa tccccgatca tgactcacca cgatgaatcc
    28801 ttgttttttc tttttcaagt aagccgctac ttgttttctg ccagagatat ccaaatgatt
    28861 cgttggttca tcgattaacg ggaaatgagt gtcatccaca aataagagtg ccagtaggac
    28921 ttttgtcttc tctcctcctg atagtgtgct gaattctctc cataagattt caggatctgt
    28981 ttgcatcagt tggagctctc tttcaatttc ccatatctca aaatccataa tctcatttaa
    29041 cacataatag gtcaaacgct ctttatcttt tgtttgctgc gggaaataga caaacgcttg
    29101 ctgatggatc acttgccctt gataagggag tttgttttgc aggatattca gtaaagttgt
    29161 ctttcctcga ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa agtttagatt
    29221 ggcttgttca aataataatg tgccttggct gtcatagccg aatgtcagat tttttatttc
    29281 gattttagac attattccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    29341 taaagaggat gcaagggttg gggcacacaa aaagacccat acatcgaaag aaccaagcca
    29401 atcccgaaaa tctgcacact gctcccttgt ctccacaagg tacagtctga atgcagcttt
    29461 catcaatcga cttaattttt caatgcacgg atccctcact ttgtcctata tttgaaatta
    29521 cgatagcata aagaaattgt ttttgtcaac tacttcggga gaatgttgaa aaatctgctg
    29581 agtgtctgtc ctaagatttc tgttttttct aatgtttgcc tgtgttccat tgcttcctct
    29641 aaatcgattg gtcctaattg aatgcttaag acaatgggga gaaaatcaaa gagtccttcc
    29701 agctctcggt ctctacttcc gcaaatagcg attattggtt ttcgatattt ttttgcaagc
    29761 actgctacgc catatggaac ttttccgtgg attgtttgct gatcaatccg cccttctcct
    29821 gtaatgatga catccgcttg ttcgatctgt ttttctaatc cagtgatttg gctgaccaac
    29881 tcaaaaccag agaccatctt cgctcctaac aaaaaaaggg ctccgccaat accgcctgct
    29941 gctccgcttc ctgggatgtc atctaaatcc acctcccaaa catcttttat ctgctgagca
    30001 atcttttcag cttgtttatc caagaaagct aacgtcgtat ccgttcctcc tttttgtctt
    30061 ccaaattgcc aagcagctcc ttgttgacct gtatatgggt tggtcacgtc tgcagcgatg
    30121 atcactttta ctttagaaac aagaacgttc cttcgatcca aatgaagttt tctagtagat
    30181 aacaaaggat ttccttctgt gtacccttct aaagaagcac ccaaagattg gagtaatcct
    30241 agaccaccat cgctacatcc gcttcctcct aaagtgacaa tgatttgttc cacttcttga
    30301 agcagtgtat ctttgataag ctctcccaag gcataactag aagcaagtcg gatggtttga
    30361 tccgttggtt tatcaagtaa atctaatcct aatacttgtg cagattcaat gactgcggta
    30421 ttttttcctt gccagttcgt caataaataa ctgatttttc gctgacgaaa taagagatcc
    30481 atcccatcaa tcgtacgcca ggttcccccg agcgcgtaat agattgcttc tgcagtattt
    30541 tctccgccat ctgcaatcgg cacattgatt tttcttcctt tacggtcttc ccaaccattt
    30601 agaactgcct gatttaattc aaatgaagaa gcacttcctt taaatgaatc gattgccgcc
    30661 aaaatatcca tctatatccc tcactttctt cttaagtaag acactttttt tcactttttt
    30721 caaacaaaac aatgctattt tcttctgttt ttctatatac taaaagggaa ctcattgaat
    30781 aaaggagaga aaaaacatga aaatcgcaat agctggagcc ggagcaatgg gcagccgaat
    30841 cggattaatg ttgcatcaaa gcggaaatga ggtcgtcttg atcgatcgct ggcctgctca
    30901 cattgaagcc attcgtacaa atggattgat tgctgacttt aatggcaaag aagtagttgc
    30961 aaaactgcca atctattcac cagaagaaat catcgaaagc aacaaacacg tggatttgat
    31021 cgtagccttg acaaaagcga atcaattaga tgatatgttt cgttcgatcc aatccattat
    31081 tactgagaat acttatgttt tgtgtttact gaatggatta ggtcatgaag atgtactgga
    31141 aaaatatgta ccaaagaaaa atatcttatt tgggatcacg atgtggacag ctggtctagc
    31201 tggtcccgga aaagtcacat tattaggtga tggagaaatc gaactagaga acttggaacc
    31261 agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt caagaagcaa aattgaatcc
    31321 aatctatagt cataatgttc gttattccat ttggcgaaaa gcttgtgtaa acggaacatt
    31381 aaatggatta tgcacgatat tggattgtaa tattgctgaa ttaggtgcgc aaaaagcggc
    31441 agagggcatg gttcgaacga ttgtatctga gtttgcttct atcgcagcaa aagaaggaat
    31501 cacattagat caagaagaag tgtaccaaca tattgcttct acttatgatc ctgacaacat
    31561 cgggttgcat tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat
    31621 cgactacatc aatggtgccg tttggcgaaa aggacaaaaa tatgatattg ctacaccata
    31681 ttgtgccttt ttgacccaat tagtccatgc aaaagaagga atccttggcg cagaataaaa
    31741 ggctctctga tgagaatcag ctggatcagc agctggttct cattttttta cccccatgaa
    31801 agttcaaaaa gaaaaacaca attctcatgt tgccataaga attgcgcttg tttattttca
    31861 tctatgattg gttggcactt ttatttattt ttaacagagg aaagatcgta ttcatattgt
    31921 tcataaatag cggagtcagt agtgaaaaaa cgttttgccc aattttctgc atcaaaaaat
    31981 tccaacagtt ctgatgggat tggttgaaat ggcgtatgca aaaattccgg taattcatct
    32041 aaatcacgtt cttgaagcaa aaaaatattt tcagaatgat aaaactgata atgagctatg
    32101 gcttgattat ctgtcactag tttcttttga aaatacatag attcaaacgt tcgcaaagtc
    32161 actccttgct gcccttcccg caacagttct agaatccccc tcgattcccc ggtttttttc
    32221 agatattctt cataagtaag gtatccaggt tgttcatttc ctcgaagcgg aagaataaag
    32281 taattggtcg taacacctct ttttgtaaat tcctgcttat atttttctgc ccgttctttt
    32341 ctgccgttat ttgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaatcc
    32401 atatgtttcg aataaaagga actattatgt tttagtccaa gttctttcgc ttctaaaaaa
    32461 tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg
    32521 tgttctgctt taaagtggtt ccaaaaaaat aacacgattt ttgcattggt aaatggacgc
    32581 acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt
    32641 gccaactgtt gtcgagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa
    32701 acatctaaag agcggatgga aggaaaaaaa taaagtgatt tatttttcgc aattagcaat
    32761 acgtcgtttt tgttcatctg ttccgcctca ctttctctgt cactttttcc tatgatagcc
    32821 tgtttaaata gtaaaaagcc agtaaaacgc ttcctattat ttttataaaa atttaacatt
    32881 tctatgaaaa tacgatgaaa taaatcattc ttccattaaa atatgcccaa aagttttctg
    32941 aatagttgta attatctgaa aatttgttat cattagtcat tattattatt actatttatt
    33001 cttactattt attcttacta tgcattttga ttgtgaattt aaaagaaaga ggttatttat
    33061 ggaagataaa aaattgaaaa aacaacttag ctcccgacat atcaccatgc tagctttagg
    33121 tggagcgatt ggtgcaggtc tatttaaagg aagtggcgaa gcgattggaa tcgccggccc
    33181 ttctgtattg atcgcttttt tgattggcgg cataatttta tatatcgtga tgaaaggtct
    33241 gggaaaaatt gttttaagtg gtggtgatac gcaccacgga ttatctggat tgattcgtcc
    33301 atatctaggt gctcatgctg ccgattttac cgattgggtc tactggtcca tgtggattat
    33361 caatatcatt gctgaagctg ttgcagctgc ttccttttta cagttgtggt tcccccacat
    33421 cccagcttgg gtatttgttt ttatgttggc tgttttaact actatcatca atctttattc
    33481 ggtacgatta ttcgcagaga cagaatattg gctggccttt gctaaaatca gtgtcattat
    33541 cctattgatc atttttgctg cttatcttgt cggtcagcaa atgttgggta ctggtgtttt
    33601 ccctaccctt caacaattaa ctgatcatgg tggttttact ccccacggta tgaagggaat
    33661 catcagttct ttgcttgtag ttatctattc ttacggcggt tctgagttga ttgctatcac
    33721 agtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gcgtcatggg
    33781 aagaatcatt tccttttaca ttattccttt atttttgctt ttgattattt ttccttggaa
    33841 tactttagca ggcacgaacg ttagtccttt tgtgatggta tttgaaaaaa tgaatattcc
    33901 atttgcggca gatatcgtca atttcgtgat tgttcttgct ctgttctcgt ctatcaattc
    33961 aggtgtatat gcttcttcga gaattttgtt cttccgcttg aaagatcgga aaggttcctc
    34021 tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgtgc tgttttgtac
    34081 cagtgttctt tatctaggtg tggctctttc ttattttgtc ggtgacaaat tatttggcta
    34141 tctggcaggt tctctttcct atacagtatt attgatctgg atcattatca gtgctgcgtc
    34201 ctttgtactg acactaagga aaggaacgtt atgggacaaa ggaatcagct tttttgcatt
    34261 agcagtctta tggttgattt tccttggtat cctattcacg aactccattg gggtaactgt
    34321 attgaccggt ttgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcttt
    34381 cgtcttgacg aatgaatgaa accaatagag aattttattc tatttatatt gaatatctgg
    34441 agctattgaa aaaaaggagc tgcgacaaaa attgttgcag ttcttttttc cgaataaacg
    34501 gtgttcaaaa accatctgtg cagattaatt gattgttctc cacgctcatg acatgataga
    34561 agaaaggaat gaagaaaaag gagtttgttt taaatgaaaa agtattggaa gtttttgttg
    34621 atgattggtg tttcgacggt agtcatgttt ggtatgatgt atttgaatgt ttatgcgctt
    34681 gatcatatct tcttcagtcg cacacgtgta ttcatggcgt tgatgatggg tgcagtcatg
    34741 gcagtcatca tgttgttgtt catgtggaaa atgtatgaaa acaaaaagat aaatgctatg
    34801 attttagccg ctagtcttct tgtttttgcc ggttccttat ttatggtacg aagccaaaca
    34861 actgtcaacg atacggcgtg gatgaaggcg atgatccctc atcactctat tgctatcttg
    34921 acaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga agaaatcatt
    34981 caggcacaag aagaagaaat cgacaaaatg aaaaaaatga tcaaagaaat ggaatgacca
    35041 ttatccatcc aaataaaaaa agaggatgtt ccagaagtga tcaacttcaa gaaataaaaa
    35101 gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta
    35161 ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttagg cttattaccg
    35221 caaatgcaag cttttgaaca ccgtttattc gtaaaaggga gtgtgacaaa agtcttgtca
    35281 cactcccttt ttacgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg
    35341 tttcacaaat ttctttgaga tcccccaacc tgtaaaggca ctgaccatcg aaaacacagg
    35401 tgaaagcaaa ctgaaaaaca cgaatggcaa gtaagtgagt gtaggaatgc caaatgtact
    35461 tgctacgaaa cttccggcga ttccccaagg cactaagtag ttgattactg ttccaccatc
    35521 ttctagtgtt ctccctaata cagttggatc aaatcctgct ttttcgtaag tttctttaaa
    35581 tgcattacca ggcaggataa cagatagaaa ttgttctcca ataaagatat ttactccgat
    35641 gcttgtcatc aatgttgtaa aaatcaattt tggggtgttc gtcatctttc ttgatactcg
    35701 ttccataacg gttgagatca acccaaattc gatcaacaaa ccacctaatg acaatgttag
    35761 aataatcaat gacacagttg gcatcatgct ttcgattcct ccacggctaa gtagtgaatc
    35821 gatttgttgg tttccagtat ttgaaacaaa tcccgattcg atgacccctg ccaacttcgt
    35881 taaggaagtt tgtgggtttt gaataaagat cattcctact gctacagcaa tattcaacaa
    35941 aatcgtcaaa attgccggta cctttttcca tgcacacaag agcattaaaa atagaggaat
    36001 cagtgaccag atcgaaatag taaagtttgc ttctaaaata tcagtcactt cacgaataga
    36061 tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac
    36121 aaatgccgga acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac
    36181 aatagctgct gctaaatttg ttgattcgga taatggagac atcttgtctc caaaaacggc
    36241 acctgaaaca atggctccaa caacaagggc tggatttagt cctagtgttg tcccaatacc
    36301 gaaaaatgca atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac
    36361 gattgcacaa acaacaaata cagaagggac gaaccactgg acactaatca atttaaatcc
    36421 aatgaccatg agggttggaa taattcctgc ttgtatccat acagcaatca atgaaccgac
    36481 taaaataaaa ataaagattg gaatgatccc cggtttgatg ccattgatga tcccttcgtg
    36541 cagccaatct gtcgggtatt tttttatcac tgcgtacaac atgaccattc cgatagctgc
    36601 caaaatcgtg atattaggtg aaacctttaa tccgacaacg ccaatcgcaa tgcctaaaac
    36661 aatgaccatc aatacaagtg ttgcttctaa aaaacttggt gttccttttt tttccatgat
    36721 aatctctcct tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg
    36781 ccacaattca tccgcgattc ctcctttata aaaataaaaa gacctgcttt caactaagga
    36841 cgaatgactc cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacgat
    36901 tattcaaatg tatggacttt ccctgtaatt cgtcaattag gcttgatcgg ttcacagcga
    36961 ccaccgactt tctgaacaaa acacctaacc actactgcgt gaaaagttga cttgtatgaa
    37021 gtttaccttc cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa
    37081 aattattttt ataactttcg cgcacaactt tatcttttta ctagaaagta gcgccctctt
    37141 gtttttttct tgctactatt tcttgatgtt cttgtttgat tttttctaat agcttgggtt
    37201 ctctcaataa atctagagct gtattggcaa gtaattccgc tgcaatcgca atagaagcaa
    37261 gtcctttttc acttttagca gcagctttga attcttctgt atgtccggca atatatgtct
    37321 cagagattgc tacagtaggc tgtatcgttg gaataacttg gctaacattt cctacatcag
    37381 aagaacctag acttgttctt tgttccgttt ctatttcttc atcaggaacc cctgcttctt
    37441 ttgcatgttg gaagaataag tcatcgaaag aaggcgttac gatgacatca tcgactgcat
    37501 tttggaacaa tccaaactca aaagtcgtac ccgtgcttaa tgcagcacct cgtacgatat
    37561 tttccacttt ttcatatact ttgtctaatg tttgtcgatt tcctgcacgt aaatagaaac
    37621 gtccagcagc gtagtctggt atgacattgg ccgccacgcc tccatctgta atgattccat
    37681 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccgtta aacacttgga
    37741 tcaaggcttc caaagcattt actccttttt caggtgctgc tgctgcatga gaagcgacac
    37801 cgtaaaattt gatatcgact ggatcatttg ctaaagattc tgtcgttttc ccataacgaa
    37861 aagctggatg gacacataat gctgcatcga catcctcaaa aaaaccttcc cgaacgaagc
    37921 ttcccttagc tgatccattt tcgcctcctt cttcccccgg agtaccatac acacgaattt
    37981 ctccgccaat ctcatcaatg aattgtttca ctatgctggc tgctaatacg gagtaagtac
    38041 caaataaatt atgtccgcat gcatgaccaa tacctggtaa agcatcaaat tctgccaaaa
    38101 aggcaattgt cggacctggt ttattggaaa tataacgtgc atcaaagcct gttcggtgcc
    38161 cagctacttc tttttctacc cagaaccctt ctttttccaa ttgtttgatt aaagctgctg
    38221 aggaataaaa ttcataatta cttacttcag gatggtcgtg gatagctagt gcaagtgctt
    38281 gatattctgg taaacggtat tgaatcagat ccttcaattg atttgttgag agtgtggttg
    38341 tcataaaaat ttcctcctat gctagtcgtt ttttcttatt ttctcccgaa tgtttcccac
    38401 gcaggtacgc ttgaaccttt tgatgttttt tcgattactt tggctgtttc ttctgtttga
    38461 taggcatcta ctattttttg atacgtttta ttgtcttcat cttcttttct tgcaacgatg
    38521 atattgacat aaggacgtgc atgctcatcg actggttcta agaagattgc atcagaagcg
    38581 ggattaaatc cagcatctac agctacaccg ctattgatga ctgaaatatc tacatctgac
    38641 aaagcacgtg ctgtttggga agcatctaac tcagaaatgt ctagattttt tttattctcg
    38701 gtaatatcag aaacggtagg cgcttggcct tttgccttgt ccactttgat caatccagca
    38761 gtttgcaata acagaagagc tctgccgcca ttcgtgacat cattagggat tgcaactttt
    38821 gctccatctt tgatatcagc cacatctttt aacgtattgg aataaattcc caacggtgca
    38881 ttaaccgtat tcccaatcga tactaaatca gttccgtgtt cttcattgta attgtctaaa
    38941 aagaattgat gttggaagga attcaaatcg atcgatccat ctgctaaagc cgcatttggc
    39001 tgattgtaat ctgtaaattt cacatattcc aaatggatgt tttccttttt caaacggtct
    39061 ttcacatcgt cccatacatc tgtatcttcc ccaatgatcc ctagttttac ggtcgtttct
    39121 tttttttcag ttgtagctgc ttttccttgg ctgcatcctg ccaaccaaat aattgctaca
    39181 aatgtaatga ctgcaaaacg aataattttt ttcatttttt ctcctccatt ttcaatgtgt
    39241 gactttttta atcagttgat tactgataaa ttgactcaaa aaaactaaga ccagaatcaa
    39301 taatgtagcg acaatcgtta catctgtttg gaatcgatta tatcctctcg atatagcaag
    39361 attccctaat cctccggcac ctactgctcc agccatggta gtcagtccga tcacattgat
    39421 gatcgtcaac gctgagaccc gaatgatacc aggtaaccct tctaacaaat aaacacgtaa
    39481 aatgatgccg attgggctcg tgcccatcgc ttctgctgct tcgatcaccc caggatcaac
    39541 ttctagcagt gcattctcga tttgacgggc aaaaaacggg acaacgccaa aaatcaaagg
    39601 gactaaagct gctttggcac caatcgtcgt tccaactaag aaacgagtag tcaaagctaa
    39661 aagagaaagc aagataatga aagggatgga acgaatgata ttgatcaact tatcaagtat
    39721 ttcgaacagc agcctgttag ctagtaatcc atttggacgt gtcactacta acgtcacacc
    39781 taagatgatt ccgatgatcc caccaatgaa tgccgtccaa aaagacatat atagtgtttg
    39841 ctttgtcgct tcgatgaatt catccggaat caaatagaca ttcggcaaat atgttttaaa
    39901 aaattcagtc attttagttt cctccttcga tgactttgat acggttatct aattcatttt
    39961 tttgtaaaat agttactttt acaccagatt taatcaaata atccaatgct ttttgccgct
    40021 gtttggtttc tccagctaaa gtaacgatca aacttcctaa gggtgtttct tgtaatagct
    40081 ctacattacc gtataagata ttcgctgtta cttggtactt gctgtaaaga tgagcgatta
    40141 gaggttcgtt cgtctggttg ccgatataac ttaattccac caaccattta tttgcaatct
    40201 gatcggcaaa agcagcatgg gcactgattg tttccaatgc ttggtcaagg tgtgtcgctg
    40261 tccgaataaa atctttagtc aattcttctt caggatgact gaaaatctgg atgctgctac
    40321 ctttttcaat gattttcccc gcttccataa ccgctacctt gttgcagatt tctttgacaa
    40381 cttgcatctc atgagtgatc aggacgattg ttagacctag ctgacgattt agttttttta
    40441 acagtgcaag gatctgaagg gtcgttttcg gatcaagtgc actagttgct tcatcgcata
    40501 acaacacttt aggttcattt gccagagctc gtgcgattgc cactcgttgt ttttgccccc
    40561 ccgacagttg tttaggatac gcagatgctt tttcttccag accgacaagt tccagtagtt
    40621 cattcacttt ttgacgacgt tcctgttttg attttccaga atactttaga gaaaaatcta
    40681 cgttgtcaaa aatcgttcga ctttccatta gattgaaatg ctgaaagatc ataccgatgg
    40741 cctttctttc ttttcgcaat tcttttggag agagctggct taaattcgtc tgattgacga
    40801 tgacttctcc tgctgttggt ttctgcaaca aattcatcac gcggactaac gtactctttc
    40861 ctgcacctga gtaaccgacg atcccataaa catccccttt ttcaatcgtt aaatctacag
    40921 aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaata tctcgcaatt
    40981 caatcattaa cattcctcct tgtctataaa aaaagcatcc tcaagaaaat gaaaatagtc
    41041 attttcttga ggacgccttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac
    41101 aactcctctt caagtaccat catactctag caagatatcg gtcgcagccg tcaccgcctt
    41161 acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct
    41221 tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa
    41281 ataatattca aaaaaaaaat ccttttacta acataaacta tgtcagtaaa aggacgacgt
    41341 atcgtggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag
    41401 agattggcgt tcgttttaac ggtcgaacat ttccgaattt gcctacttat cgacaaatta
    41461 ttctccaagg ccattttcag cttttcttac ttgactcttc acaccagcca gagcctctct
    41521 gcaaagtttc caagcttact ttccttctca acgaaatcaa tattttgttg ttgggaataa
    41581 tctacaggag gaaaactgaa aagtcaagca tgaaatccaa attatttttt ctatatttat
    41641 ttttctgttt ttttatctaa catttcaata acagttcgaa gatcttttcc ttccgcaaga
    41701 aggttcaatt tttccttctc ttcgacgcta taatttcgaa caaaactgga atcgggaatc
    41761 aaaacaacgc ctagtcctgc acgtgaagcg gctgtcgctc ctactaaaga atcttccact
    41821 gcaaaagctg aaagagcttc taatcctgtt cgttccaaag ctgtcagata agggactggt
    41881 gaaggcttgt tctccgatac ttcatctccg aacgtaaacg tatcaaagta atcggctaat
    41941 tgaaagtgat ccaagatatc tgttgctctt ttcgtaacag ttgtcgtagc aatccctagt
    42001 ttcatatgac gttcttttaa gtctgtcaat atttcttttg catagggttt caattggata
    42061 gctccacttg tcagctgttg ataaaaatat tcttcacgct ttgctcggac ttcctgcact
    42121 ttttcaggac tgcctgctgc ctgcactaaa atatcaaaac tttgtttcca gttttttccg
    42181 ccccaagcgt caacgatttc tttcgggata tgaatatcaa attgctggaa tccccaaagc
    42241 caaccatccc gatacgttct ttccgtatcc actaacaaac catctaaatc aaatattgca
    42301 cctttcatct atattccaaa acaatacacc tagtttagca tgactgatcc aaaatgaaca
    42361 gaagcataga gcccagccgc atcacttata agagttcttg gaatcgacgg atatagattt
    42421 tcttcacagc tgtcacaagc gccagataag cgacgactgt tataaacaac cataaccagt
    42481 aattgccagg gagcggcatc aagcctaact cagcaccaaa tcctgtgaat ggaaggactg
    42541 aaccgacaat gattcctaat gtagtgataa ccagcatggc aaatgttgca ttgctttgga
    42601 taaatggtat tttcggtgta cgcaatgcat ggagtactaa tgtttgtgtc cacagggatt
    42661 cgacaaacca tcctgcatgg aataatgcga taaaaatcac ttgttgtgag ctatccaatg
    42721 tatggaaact tccaccaact acagcaggac aaataacaaa atacatcaac aagtaagtag
    42781 tgatatcaaa aatcgaactt gttggaccaa aataaaccat gaattttcca atactagatg
    42841 cattccattt ctttggctca tccaaatatt ctttatccat ccgatcccat ggtaaggaaa
    42901 tgcaagaaac gtcatagatc agattcaaaa atagaagttg caaaggcaac attggtaaaa
    42961 atggtaagaa cgtactggca atcagtacgg aaaacatatt gccaaaatta gaacttgctg
    43021 ttgccttgat atatttcatg atgtttccaa aagtttctct tccagaaaga aggcctcttt
    43081 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc tgattctttt gcgatatcca
    43141 cagctgtatc cacggaaata ccgatatcag ccactttcat tgctggagca tcattgatac
    43201 catctcctaa aaatccgaca gtatgcccat ttttacgtaa agctgtcgtc aatcgtgctt
    43261 tttgctgagg attcaatttt acgaatactt cgtgttcttc agctacttgc gctaattcat
    43321 gatcattcat ttccgagatt ttctcaccag taatcaattc attgatttct aggcctactt
    43381 gacggcacac agaccgtgtg actaattcat tgtccccagt cagaactttt acagctacac
    43441 catgttcttt taaagctttc aacgcttgtt ttgttgtttc ttttggtgga tcaaggaaag
    43501 caagataacc aatcagaacc atgtctgact catctttgac tgaaaattct ccaacaacgc
    43561 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc gtcttcatta agtgattgta
    43621 cttttgcaag caccgcttct tttcgttctt ttgttaatgg tgcgacagta ccaccaagat
    43681 caataaattt agaaatgttc aacatttctt caatcgcgcc ttttgtgatc atctgtgttt
    43741 tgccagccgg atcagcgact acaacactca tacggcgacg ttcaaaatca aaaggaattt
    43801 catctacttt ttgataattg attgtatctg tatctaaagt ttctgctgct tcatcaatga
    43861 tagccacatc taacaaattt ttcagccctg tttgatagta gctgtttaga taagcatggc
    43921 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttgt
    43981 cttgtgtcaa tgttcccgtt ttatccgtac ataacacatc gatcgcacca aaattctgga
    44041 tagcatttaa atttttgatg actgttcctt ttttggccat cacacttgct cctttgacca
    44101 aattcgtcgt aacgatcatt ggaagcattt caggcgttaa accaactgcc acagataatc
    44161 caaaaagaaa agcttctaac caatctcctt ttgtaagccc attgataacg ataactgctg
    44221 gagccatcaa tgacataaat ttgatcagca gcatggatgt tttgtggatg cctaattcaa
    44281 aacttgattg gatgggtttt tcagatagcg tttctgctac atgcccaaat agtgtgtcat
    44341 ttccgacaga aacaatgatt ccttccgcgc ttccactgat gacattgctt cccataaaga
    44401 caagattttc ataacttgtt tcactttgct gatcgtcttc acatactttc gcatgctttt
    44461 ctactggata gctttcccct gtcaatgctg cttgagaaat gaatagatcc tttgcggaca
    44521 ataaacgcat atccgctggt atcatatctc ctgctgaaag ccgaacaaga tctccacaga
    44581 cgatttcttc cataggaatc tctgtatact cgccatttcg tttgacagct gccgttactt
    44641 tcacaagtga tttcagtttt tcagctgctt gattagaacg cacagactgg atcaacgtca
    44701 tcgtcccact gacgataacc atagtgaaga taatggctac accaaagaga tctttatctc
    44761 ctggagcggc taatacgtat tccgtaatga atgaaataat tcctaatgca atcaacacta
    44821 atgtaaaggg tgtgatgtat gctttcaata tttctataat gaatggtgtt tttttgccat
    44881 atgaaatcgc attttcacca taagtttctc ttgaacgctc gatttgttct tctgacaatc
    44941 catttggtga tgcatggaac aattcaaata attcttgctt cgtttttcca gcaaaagctg
    45001 catagatttt ttgtgtatct ttcttttttt ccatctcgat ctctcctttg tgtccaaaag
    45061 acagctgttc tcgctgtttt tggtcttttg ctattcatcg gatcgataga gaaaatgctg
    45121 aaaggggctg agaacagata gataatagcc ctggcctttc ctctaaatcc ttgtttttcg
    45181 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    45241 aatcattttt cgaatagtta ttttcccaac tcgctcacct ccctttttgt tttttcacct
    45301 atgggatttg cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag
    45361 caaatcaaaa aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa
    45421 gcagatatct gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc
    45481 cattggcgtc tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa
    45541 caaatcctat tcggttttcc ctcgttaata taacaactaa aaaagaggat gtcaaatcaa
    45601 ttttccatgt ttttcatact tttttaaaat acacgcgtta tatttcctgt ctcacgcagt
    45661 tttatttgtc tcattttcca cctcccgcta taatagaatt atagtaggaa acgaggtgct
    45721 gttatggaac gaacacttgt tattatcaag ccagacggcg taagacgcca tttggtcgga
    45781 agcatcatcc aacgttttga agcaaaggga ctggccattg ctgaaatgaa atttgcaacg
    45841 atgacacctg aactagcaaa agaacattat cagcacttga ctgaacgtag tttttttgat
    45901 gagcttatcg attacatgac ttctggtccg gtcgtttatc ttgtgctggt cggcgaggaa
    45961 gtgatcgata tcgtccgtaa gatggtcgga gcaacaaaag gggcggatgc cgttccaggt
    46021 acgatccgag gagattatgc actgccgggg actgaaaaca tcatccatgc ttctgattcc
    46081 cgtgatgctg caacaaaaga gatcgcccgc tttttcccgc cattcgatac aaacaaaccg
    46141 tctgaaaaag cggcgaagtg atacaaatcc taaactgttg gtctaactgc tgggtttagc
    46201 aatggccagc agttttttct ctaaagaatt cctttgattc ttccctaatg tatattcgat
    46261 cactgcgaaa aactcttggt gattttccga tttttcttcc cctaaagcag cagaaatcag
    46321 tcgttggtaa taatccgcca tcaatgtttc ataaatcact gtacttcttt ctttatgcgc
    46381 taaactccgt ttcatatctt cattcacgct acgtgccttt tttaagcgac cattttctat
    46441 ataaaacaaa gtcaagttcg tcaaaaaagt aaagagatac tgctcgtgat tattcagttg
    46501 cgcgatccgt cgcattcttt ttttgatttt caaataatta ttctcaataa aacgatcact
    46561 gaatatccaa ggaaaatctg ctagaataga tacttcaaat tgcccatact ctggtacttg
    46621 catcaaatat tcttcaatga tacgacattc ataacgcaaa aattcaaatt ccgtctcaga
    46681 tacttctgtt tttttggaca tgtgcagatt caaaaccaaa tagtacagat cttctgtttt
    46741 cttatagtgc ctgcggttat tagcaatttc tttgtaagga tcaaagctgt cttcttgaat
    46801 ccgatctagc attcgtcttt ttttctttaa tttttttggc tcatcaagct gatttcgtat
    46861 ccattcatat tcggcaagtg tcatgttcat tcgctctaaa aatgatttca atgttccaaa
    46921 cgatactttt ttcactgaat ttgtctcgat cccgactagc gaagttttag aacagattcc
    46981 ttccgctaat tgggtctgtg tcagcttttt gttgactcga agcttcttaa tcaattctcc
    47041 atcatacata atagttctcc ttttttaatt gaaattaacc cttggctttt ccagaatgct
    47101 tgccaataag aggaatgatt atttcaattt aaattttccg cattcaataa atagatacgt
    47161 taaaaataaa aaatacacga aaaaaataaa aaaatgtttt tctcataaaa aataccaacc
    47221 ttcaagcatg agactgctgt ctaatccctg aaggttggtt tgattgttta ttgatcattt
    47281 ttttactgct ccgcattggt tcctgttact ggatatagga tagtcgttgt tttattgccg
    47341 ttttttgaaa agacactggt cgaatgttca cctttttgct tttcgattgc ttcattacgt
    47401 tccgcttcgt ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca
    47461 taaaaccgca agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact
    47521 ttttcctgat ctttggagat ttcctcgtcg attcctgctg ctttcgtatc gatgatttct
    47581 cctgtctgat tattgtaagc ttttcctcct aggacggtgt atttgggcgt cacgaagttc
    47641 ccatttcgaa aagcaactac ttgatcgtgc tgagaagata ataagtctgt accaaaatga
    47701 agataatctt tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct
    47761 ccaccatatt catgattgac tgtcccttta gtgtaaccag gcatatggat catcaatggc
    47821 actcgttgca tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagca
    47881 cttgctaaat ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag
    47941 attgaatttt ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga
    48001 tccagataat gggccgttcg aacataatta tctactgtgc tattgccagt attcaaagat
    48061 ggaaagtcga aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat
    48121 ttcgtgtaaa acggctgttg tagatgttca agatattggg cagattctct gaataaatct
    48181 ttatctaata tcccataacc taacgtttca tcggattcat caaaataaga tcgatcaaag
    48241 aaattgtcat accctaaatt tttatataca tgatctctat tccagaaact agctacattc
    48301 ccatggaaaa cagcacttgt atagccttct gtttgaccaa gggtcgctgg tgctgcttga
    48361 aagacattat ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca
    48421 gtttccaaca tgttttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg
    48481 tcaaaactga tcgttgcttg attctggaaa atactattta aaaacggcgt gacttcttgt
    48541 ccgtctacct tcatattgat caaaaactgt tgaaaacttt ctaaatgaag gacaatcaca
    48601 ttttttcctt tagcaatccc aaatgtttca ggatttggct ctgcataatg tttctttgta
    48661 tagtctaaaa cttcatcaat tccattactg cttgcatgcg cgcgaacact gctagtcatt
    48721 cctgttttga ttccgtcata aatagtgtac gcatcgattc ctagatattt gacaatatag
    48781 gaacggtcaa atgtccgctg tagcaattgg ggacggttgg cttcgctcaa cgctaaattt
    48841 cctgaaaaaa tcaatcctga aagacaggta acagcgatag ccatcggttt gctgacaggg
    48901 ttgcttttga taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg
    48961 atatccaacc aatagatgac atcatggggt ttcatcaaag aaaaggagct tcctgacaac
    49021 ccttgtgaca ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc
    49081 cgataaaaaa taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga
    49141 atggctaacg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta
    49201 gtcgcaatcg ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa
    49261 aaatctgcat agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg
    49321 attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat
    49381 tcacattttt tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt
    49441 aagatttttt tattctttct aaagattatt ccattcattt ttcacagcat aaaatcgttt
    49501 cggtgtaagc ggttaaaata gtaaggagat aaaaatcttg tggtgaccga tttactctat
    49561 agtattcaaa agtagtttct cggtattcgt acaaaaaaaa catactaaga ttagagggga
    49621 acatcattga cgaatgatgt tctcctctat tttctatttt tagattatag tgaagagtga
    49681 aagaaagttc gaacaaagcc ttgaggacta cgagtcccta tcaaaaaccg aacttcttta
    49741 ctgtcatttt agaatcaggt ttacgtatgt tggggccatg agcccgtgta taggaaattg
    49801 gaatcgaaat gaagcagtaa agttctttca acacttaaaa aggaggaaaa caaatgcctt
    49861 acattcttgc attagatgtt tcaatgggac acagctacag tgtcctatac aaagacgaca
    49921 cctgcttatt tgaagacgag atcgaacaca ctcaaagagg atttcatgca ttactcgaag
    49981 agatccagca cttgcctgaa cgtccattga ttgtttttga agcaacgggt atttattcca
    50041 gaccagtcga aaagttcttt caagatcatc acttttctta ctgtttgtta aacccgttag
    50101 aagcgaaaaa acagatggaa gaaaacacct tacgcagttg gaaaacagat aaatcggatg
    50161 cccatcggct cgcacagact catcttaaaa atcaacgtca accaaaagaa gtacaaagca
    50221 atttttactt agagatgcgc gatttagccc gtttttatca agaaattgag aagaaaatca
    50281 ctcgactgcg catggattta cacaactgcc ttcagttgac ttttccagag cttgaacagt
    50341 tcttttcaaa caggctgacc ccttacgcat tgacattgat ccgtcttttt ccccatccag
    50401 attttgtatt ggcatctact cggacaaaaa taaaaaataa actgatcaac gaaacacggg
    50461 aaaaaatctc cgcgaatcgt gccgagcaaa aggcagatca aatcatccac tatgcacagt
    50521 gtgcctatcc agctgttgaa aaagacagta ttcactgtca aaaaacgatc tattacgccg
    50581 aacttctcca agatttactc gaacaaaaaa aagcactggc gacacaaatg atcaaaaaag
    50641 ccgaagcctc tccttgcttt ttgttgtacc aaacgttccc tgggattggc gcactcacag
    50701 ccgctttatt gctcggtgaa ttaggagata tcacacgatt taaaacccat aaacaattga
    50761 atgcctttgt cggaatcgac atccgacggt atcattcagg taagtatact ggccaagacc
    50821 gcatcaataa gcgtggtaat cccaaagcac ggaaaattat tttcttcacc attcgaaata
    50881 tgattcgcca gcaacgcgca gctcctaatc atattgtcga ttattactac aaattaaaaa
    50941 agcaacctat ccctaagaaa gataaggttg ccacagttgc ttgtatgaac aagcttctga
    51001 aatgtatgca cgctatggtt agggcacata cagagtacga ctatgcgtac gcggtctcag
    51061 tggaccatta actaaaccac ttacagtata ccacttcttt gcccagatcg aaaatttttt
    51121 acgaacgggg ctttatttag tgtgccttga ttttgtcgct ttcgtttacg agctattttt
    51181 cttgcacctt ttacttgact aatcgtagga aagaggctgt gacaaaattc ttgtcgcagc
    51241 tccctatttc ttgatgcagg acagcttttt cacaacctct ccactaaacg gtgttcaatc
    51301 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt ttcccgtttt
    51361 tttctctaat actcaggtca aaccgctgat ttcacaacct ctgattcacg gtgttccaga
    51421 agtagcttct tcggaaataa gccgaaattt tacaaaaact tgagaagcac ttttcgtaaa
    51481 tttcttctta tttcttgaag ctgaccactt ctgtcacaac ttctgttttt cattgtccat
    51541 aggtttgata ttgtccttct gatgactcat tcgtaatgat tgaggcttga ttttcttcat
    51601 ttcccgttat cgtttgtcca ctgccagaat ctgaagggct tagttctgtc gactcgtttt
    51661 tttgagaaga agcagttgtt ttcttgactt gttcgttggt ctttttccat tccgctagtc
    51721 gatcggccac cacttttttg tccacttttt cagaataagc tgtaaccact tttccatttt
    51781 tcaaacgtgc taccttgtcg ccatcatgag tcaaaagcaa aacgaccaaa tgtttgtctt
    51841 tgaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatattg ataaattgat
    51901 tttcttcttt tccttttggc aaatagatgt ccaattcacc tttttcgtaa ttggcagtta
    51961 atttatattc ctcccaccaa taagttgaga attctttttt gaaattatct actgattctt
    52021 tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa
    52081 atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc
    52141 tttttttcct catgccttcc tccactccta tttaattttg actattctat tatatacaaa
    52201 aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttcc
    52261 tgaagcggta agaacgggaa ttttcataca ttctctcttg gctattcttt aagcttcaaa
    52321 tccatttctg tctgatacat ctttgaacca atgacccgat ttcttgatcg ttctttttcc
    52381 atcttgtgct aaatctactg aaataaaacc gtaacgattc ttataggcat tggtccacga
    52441 ccagttatcc atgcatgtcc acatatgata acctaaacaa ttggcacctt cttgaatggc
    52501 ttgatggaca tatttcagat gagattgaac aaattcgatc cgataatcat cgtggatcat
    52561 accatcttcc ttgataaatc gtgcttcatt ttctacaccc atgccgtttt cagaaataaa
    52621 acagtcgata ttcccataat gatctcgtac attgatcaaa atatcataga ttcctttttc
    52681 atagatctcc catccgcgat aaggattcat tttacgacca ggcatttcat aattgtcgaa
    52741 gtaatcctca ggcataggac cgtttttagt atcgattggt gtttcttttg cttttattcg
    52801 gcgtggttgg taataattaa ttcccaaaag atctactgtg ttttctttaa tgatcgtcaa
    52861 atcatctgga tgaatctccg gcattagatc gagttctttt attctctcaa tcaattcact
    52921 gggaaactcg ccttttacag ctggatcaag gaatgaacga ttgaagaacg catctgctat
    52981 tcgtgctgct ttaacatctt cgggatttga ttcgtctctt ggataactcg gtgtcaaatt
    53041 taagatgatt ccgatcacac cgctttgatc catttcatgg taagctttga tggctaatgc
    53101 tgaagccaac gcttcatgat aaccaacttg gattgccttt ttcatatctg attcttccgg
    53161 ataatgccat cgatacaaat agcctgcctc gactggaacg attggttcat tatgggtaaa
    53221 ccattttttt actttcgagc caaacaattg aaaacaactt tttgcgtatt tttcataggc
    53281 ttcaaccgtt tctcggttca accatccgcc tttttcttgc agatacatcg gcatatcgaa
    53341 atgatacaga ttaacaaagg gttcgatacc gttttcaatc aactcatcaa aatagtgatg
    53401 atagaaacga acggcttctt cgttgattgt tccagattca tcaggtatca aacggctcca
    53461 ttggatagag gttcgaaatg aattatgccc agtctgtttc atcaactgta catcctcttg
    53521 atacttcgta taaacttgcg aggttttttc aggtcctact tgatcaaaga acttttgcgg
    53581 ttcgtttttg taccaataat caaaaatatt ctctcctttt ccatctcctg aaattcttcc
    53641 ttctgtttgt ggaccacttg ctgcagagcc ccaccaaaaa ttttttggaa attgatatct
    53701 tttcatcaaa gcacctcttt ttctttttta gttgattcca tgtatgatat tttccatcac
    53761 aatcactttt tcattttctt cctcttcttt tttgattcgt ttcaacagct gttctgctgc
    53821 tcgttccccg attttatctg ttggtggcgt gatcgtgatt tttggaagga tactcagatc
    53881 gtttttatct aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc
    53941 ttgattgaaa cattcgatgg caccaatcga catgttgtaa ttgctgacaa aaatagcagt
    54001 tgcccctttt tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc
    54061 tgtaaagatc tctaaatgga tagacggatc cttttgtacc gcttctttgg ctccagctaa
    54121 acgttctctt gctacgtaat ctgtttgagc ggcagcaata aatcctattg ttttatgtcc
    54181 ttgtccgcca tgatgagcaa tcacttttgc tacactttct cgatcattca ctactattgt
    54241 atcaatacct gctggcccac ccggacaatt caatgcgaca actgggatac tcatttttgt
    54301 cagcatttca aatccagacc actcttcttc tgataagaag acgatcaatc catcgatcgc
    54361 atgcttcatc aggtattcga ttttttgttc gattttttct ggctgatcgg caaacccgct
    54421 taataataag ctatacccat tttcttcagc tactctttcg atccctgaaa caacctccga
    54481 accaaatcgg ttagatatcc cgtttatcaa cagtccaata gagaaacttc gattattctt
    54541 caatccttta gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgatatttt
    54601 ttccatattc ttttccttca gctgctgacc gtttaaatag cgggaaactg ttcccactga
    54661 aacaccagcc atcttagcca cgtcacggat cgatgccata cattttcact cctgccagtt
    54721 acatttgatt tctttttctt gttgttcgac catgatgacc gctttctttg cataatgccg
    54781 cttgtcatca atatccatat aactaaagtc ttcagaagtg agttcaaaaa caagcagtct
    54841 ttcctcttga gaccgcaatt catctttacg aaaagctttc aataatttcg ttctttggat
    54901 gacaacgccg tcaattaatc ggacatagac aagagtactt gttttacctg aaacacttcc
    54961 tgtattttta actttaattg atattattac tttgtcaata tccgttttga cttccatctg
    55021 actgtattca aatgttgtat agcttagacc atgtccaaaa ggataaaatg gacttccgct
    55081 taaatcataa taattctctt gtttagaagc agcacgctga tagtaataaa caggtagttg
    55141 ccgactattt ctaggataag acaaactcaa tcgtccgctt gggttatttc ttccagaaat
    55201 catctgcgca atggcttttc caccctcctg tccaggaaac catgctgttg caatggcatt
    55261 cgtaaatgac tcgattttca tcaaatcgta gggtcttcct tgcacaagta tcgtgatgat
    55321 tggcttgcct agttgataca attgttcaat cagctgttct tggtatccac ctaaagaaag
    55381 agaagcaaca tctacatttt ctccagaatc catgttgatc cctttcgatg aaactgcccc
    55441 gttacgaaga aattccatat caaagttccg cgcacttgat ccacctagaa ctgcaatgat
    55501 cacatctgag ttttgagcaa gggtcaccgc ttctaataat ttttcttcct gatcctcctg
    55561 atttcttact tcacatcctt gtgcataaga aacctttgat tggggaaact ctgtttttat
    55621 ctcttgtact atcttttttt tcaaacggtc ttctgtttgt ggtgcagagt agtctcccaa
    55681 caagtggtaa acatcgtgag catttggacc tataacagca atgtttttta cttgttgatc
    55741 caacggcaat ataccgtcat tttttagtaa agtgatactt tcttttgctg tctctaaatt
    55801 taagtgctct gattcctcca tgagatgatc ccaatactcc tttggatcgt ttgcaaatgg
    55861 ctgttcaaat aaacctaata aaaatttgac agataaaact cggcgaactg cttgatctag
    55921 tagcttttca tctacgactt gattatcaat ggcttctgca atcttggtat aggtctcatc
    55981 ccacaaactg agatcgattc ctgccgctaa tgcatatgct gcagcagtct ttttatctgt
    56041 aaaaacatcg gataaacgat ccaaagccac accgtctgcc ataacgattc cttgataacc
    56101 gatctctttt cttaatgttt gctcaaaaag cgctcgattg atatgacaag gtatcccgtc
    56161 gatgtcatta taagcagcca tgacacctac tgcatttctt gtactcttca ataatggtcc
    56221 gtaaatatcg gagaactctc tgcgtccaat cgttactgtg cctgaattat gaccgcctaa
    56281 agcttctcct tgtgcgatac agtgctttaa cacaacaccg atctgatcct tcttcttatt
    56341 gatttcctct actgtctgat caagaaaaga aactttttct gaaataatct ctccttggaa
    56401 ccctcgaacg accgcttcag aaaaacgagc tgagagaatc ggatcttccc caaagcactc
    56461 ttctgtcctt ccccaacgag gatcttttgc cagatccaaa gtcgacacca gcgctaaatg
    56521 aacaccttta gcagccagtt cttttgccat caatcgactc gattgttcta ataatgctac
    56581 attgaaagta tttccacggc cgatattcgt tggataagaa atcccatcca agccttgatg
    56641 accatgtgga cattcttcta caagcaaaac tggaattttc catcgcgaat gctgtagtac
    56701 atattcttgg actaggttag ccaccttcca gctttctttt gcaggaatcc cattcttata
    56761 atctgtttta gaccacggat cagagcgaaa cagaccgtat aaggcaccca gaccgcctcc
    56821 ccatttaaca tgttctttta acttttctgt taattcgatc ttgtctccgt ttttttcata
    56881 acattcccaa ccatacaaat gctggtttac ctgtcctact ttttcagcaa tattcatcct
    56941 cgaaagtaaa tcttctactc tttggcggat ggttaacttt gggttttgat acgggtaatc
    57001 gtacacctta atatcctcct tctgccaatt ttgccttatt agcggctaat acaaatggca
    57061 ggaataaaaa tacatctaga atgataagta ttactgttaa gactgctgct cgtatatcgc
    57121 ctcctgtagc aataaaagat tgtataaccg gtggagtgat ccaaggtgca gcagcaactg
    57181 ttttactgat gattccaaga cttgtggctg cgtaggcaat aaccaagtta atagatggta
    57241 taatagcgca tggaatcata tatattggat tcatgactac tggtaatcca aacataattg
    57301 gttccgtaat attaaataaa caagtagtca gaccaaattt agcgattgat cgctgttctt
    57361 gacgtttaga agcccacaag atagcaataa ctaacggtaa aatacacccg ccacctccga
    57421 tcaatccaaa tgcattggta aagggctccg taataatatt aggaatttct tttcctactg
    57481 aaaaagcatc catgttttcc tgaatagact gtaacaaagg tgcttgccgt ataggagcca
    57541 aaatactcga accatgcata ccaaacaccc ataagaaatc agagaaaaac tgtaaaaata
    57601 agatgcctgg gacggataaa acaaaagctt tcaatggtgt ctgaatcgct aattcaatta
    57661 attgcggaac attcaaacca gtcgtatggt ttaaagtaaa aacgacaact gaaaaaaata
    57721 aaatgacaac tatttctggt actaaagaat taaatgacgc agctactgcc ggtggaactc
    57781 cttctggcat cctgatttta aaactcttca ttttcgctaa tttgataaat aattctgtac
    57841 tgacaagggc agcaatcatc gccgttgcta agccagacga agaagtcaga ctttgtaaga
    57901 aaacccctgt agcttctgct gtatttccgt ccatcaaagt gactgtactt gataaagaca
    57961 tcaaaataaa cattacggct gtacttagaa ttcctgcatg aacaggacta aatgatggat
    58021 catcaatcac gcctgtttgg atatagtggg ttgccaaatt ccagccgata ttgtaacaca
    58081 cgataatagc taaaatactc atcgtaccat tattcacgat tgaagcaagt tcagccaatg
    58141 gacttagatc aagtattctt tggactgttg catttgaaaa aatcagagca ttcagcaaaa
    58201 ccatgacaga agcagccatt actaacggca tagtaacgat aaaactgtct cgaatggcca
    58261 gcaagtgtcg ctggctactc atcttagata cgattggtag cattttttca gatagtctgt
    58321 ctacgacttt cattatttca tctcctagat tctaaaattg aaccggttca tttttaatat
    58381 agcacttttt ttaaagattg caacacattt ttttaaaacg ctttctaaag gtggtggatt
    58441 ttttatgact tttgaaaaat tattctattt aatggtattt tgtgaatttt ttctcttcca
    58501 tccgtttgaa gttttacttt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
    58561 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
    58621 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
    58681 aaagcggaaa aagcagatat caccaacagt gtatatacgc taaaagtacg taaagatatg
    58741 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
    58801 cagacacaag gagctgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
    58861 aagattacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaagca
    58921 acagtcacat ttaccaaaat cggtaattac cgtttgaagg ataaggatgg caacatctac
    58981 agtctatagt gccatctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
    59041 aagaagcaag atgactgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
    59101 atgtaataac aacaaaaata tccatacaga atttaatgat caaggaagtt tgagtatatg
    59161 cgacaattgc cacaaactag tcaaagattc ttttaaatcg tttgaatctt atcctgtcaa
    59221 aaattatgta aaaagcaaaa gaagataagc aaaatcaagc aaaactcact atggctgatt
    59281 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcaaaaactg gaatcagtaa
    59341 aacaaacagt ggatcatttc agttcacaaa tcaaagagta acgttttatt tatttcacat
    59401 ctttgaatat aaaataacca aacgatagaa agacttattc tacatcacgt agtaattttt
    59461 ctttcccgtc gtttggtttt ttacttttag cctaattctt gacctttcgc atgctgatta
    59521 tctgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga
    59581 cgaatcacct tttttgcatc ttctggattt tctacaaaat cgaatgtatc cccatccact
    59641 tgtattttag gacatacatc aaaatcctca taccattgat tatagcgggt gttcagtgtt
    59701 tcgtaatagg tgtataacgt ttcatcgctt tctagctgtt catagtcacg cccgcgtttt
    59761 ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggacgt
    59821 ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc
    59881 tgtacttcga tatccgttac ccgacctaaa tcagcattta gatggaacaa cagactatct
    59941 tcgtagatgg agcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa
    60001 aatcgcttat tcaaaaagaa gatttgcaat aaaaatgcgt actgctcagg atttgaataa
    60061 aaaagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct
    60121 tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc
    60181 actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat
    60241 ttttcaaaat catattcacg tacagcttgg tctgatttta aatacgtgtg aagactttgt
    60301 gtaatcatca agttcgtagg taccgtctct tcttgtacga tgatcatcgg ttttttcaac
    60361 atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca
    60421 aggatagcca aaacaaattc tgcatttgtc aactcttcca tatctttttc gtatacttcc
    60481 tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag
    60541 aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt
    60601 tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca
    60661 ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt
    60721 tggaaaagat cacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt
    60781 ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttat
    60841 caggtgtcag gccgatcgct atccaagaaa gaagttgcga aaattcctcc aatgtaccta
    60901 gacctccagg gaaaacgagg acagcatctc ctttttcaaa taataatacc tttctttcat
    60961 caattgtctc tgtaggaata aatgtagtaa catctttccc aggcagctca gtttcaaaaa
    61021 ggccatttgg atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca
    61081 ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatat
    61141 attttcctaa cgtttgcgca ataaatttgt aactttcttt attgccaaaa cgggaaccgc
    61201 aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac
    61261 acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat atagatcaaa
    61321 caccactata tatagtattt gcaaaaaaat aatttttcag ctattttaca gtaaccaaaa
    61381 aaataaataa ctagtaaata taaaattgaa acgacttact tatagaagaa attgggattt
    61441 cgtcctgcat gtcaataaca cgcattattt aaatagtcac acacttgttt ctcctattaa
    61501 tgaaaaatcc attttattct cgtgttatgt ttcaccaacg cctgatgttt ctctaaataa
    61561 aaaagccgac gaaccaaata atcgaaattt gatcattttg gttcatcagc ttgttcccaa
    61621 acctttgtat taacgataaa tagctggatt cgtaaaacta tcacttggat catcactgct
    61681 ggaagaaaat tcggtgataa ccgcgcctgt tgcacctgcc tggaaccagt gtttaatatt
    61741 ttgttcgatc gtgtactgat ctccagcgtg aagtatgatt tctttttttg ccgtatagta
    61801 acgttcttgc ccctttggaa taatacagga aagttttttt gtttcttttc cttcgacata
    61861 aagatatact aagccttttc gacaacgaaa ggtttccttt tttccttcat tctcatctcg
    61921 aggtgggtgc cgatgctctg gacaagtttg ataaggcaat aataccagtt ctttcccaca
    61981 gtatcgctca ttattttcat aaacaaataa attcaatcct tcagtctcga ttgctcctaa
    62041 tccaaaatct gtatattcga ttctttctaa ttctgtttct ttcaatacta ctcctgaatc
    62101 taggaacatt tgttgtactc ttttcttgta atccattctg ttcctcctaa ctgattgtca
    62161 agtcatccca agatatctct tcttcctctt tttcttcttg gatgacttct gcatttgggt
    62221 caactggttt tttcaataat aaaatagtta ttcccgtagc aaaagaacca gctaacaatg
    62281 caccaatgaa tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa
    62341 tacggctatc tgcgccccaa aacatgctca atccaccgcc gactgcccca ccgactcccg
    62401 ttgctatgac attgcgtgcc aagtcgttca atacgatagg gaaacatccc tctgtgatct
    62461 gacagatccc cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgaatatat
    62521 ttttacgaat gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg
    62581 aagctaaaat caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc
    62641 cttatccaga ataaatggta atccagaaac agccgggtat ctgcggtaat aagcctaacc
    62701 aaacgaaaaa tatgagaagc taatatgaga agctattttt gtttggctgt tcttattact
    62761 tgatgcaggg cagctttttc acaacctctt tttttattta tgtttcataa tcaatatggt
    62821 aaggatgcag ctccgtatgg tagaccgcct ccgaatattc aatcatttcc ccaatttgcg
    62881 tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc tgttttcaga atttcttgtt
    62941 cttcttctgt taaaaatact gccgagaaat catcagaaaa tgagtcaata gtatatcctt
    63001 ttttcgtcag caaacgatac aaagattctt tgccaaattc ttctctgctg aattgcatca
    63061 tttcttgtgg aagataatga acaaaataaa tataaggctg attgttcaaa taatataagc
    63121 gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa aggatcttcc ttctttatat
    63181 ggaccaattg caaatccagc gtttctttcc ggacttcata tcgggagttc tccaagattt
    63241 gcgtaaagct tgccgctttg gataatcgat tgtaaggacg attgcttaga accgtcgtcc
    63301 cacgaccact tttctttgcg acatattcat ctgatgccag cagttcgatt gcctttcgga
    63361 cagtgatttt gctgacttga aacagttctt ctaattcact ttctgtcggt aacatcgaac
    63421 cgactggata ttttcctgag aaaatatctt ctttaatttt atctgcgact tccaagtata
    63481 gtacacgtgt ccttttcata acgtcaccct ttgctttact attactaaaa gaatagcaca
    63541 taaaaaataa aaaaagaaca caaatcacat gtgagaagtg tgttcttttt cattcagctg
    63601 ctttaattac aagtttacgc catttgaggc aattacttct ttataccaat caaaagattt
    63661 tttctttgat cgattcagtg tcccatttcc ttcatcgtct agatccacat agataaagcc
    63721 gtaacgttta ctcatttctc ctgttgatgc actgacaaga tccatacatc cccaaggcgt
    63781 atagcccatc aaatctacac cgtctgctac tgcattagcc atcgcttcga tatgacgacg
    63841 taaataatcg atccgataat catcaataat cgtgccatcc tcttccatat gatcaatggc
    63901 tccaagaccg ttttcaacaa tgaataatgg tttctggtaa cgattgtata attcattcaa
    63961 tgcgattctc agcccttctg gatcgatctg ccagccccat tcacttgcct caagaaacgg
    64021 attttttact cctcccagca agttcccttc tgaagttgct gcttgtggat cagtctcatt
    64081 gattgccgta gacatataat aactaaatcc gatgtagtcc actggatatt cttttagcaa
    64141 ctggaagtct ttttccgttt gctctaattt ttccggatcg actccatatt ttttatgggt
    64201 acgcgctgtg tatgcagggt attctccacg tacttggaca tccgtgcaga aaaaattgaa
    64261 ttcttggttt tggatcattg ttgctgcctg attgactgga ttggcatcga tgctgtaagt
    64321 tgttgcatag ataatcatgc agccgatttg gatatccgaa cgcaattcgt ggccgatttt
    64381 tacggctttg ctacttgcaa cgaattgatt atgccatgct tggaaaatat tttggtactc
    64441 tcctgctcca ttgcttttta ctaatccttg acttaaagct ggaaagtgaa aggccgaatt
    64501 gatttcattg aaagtcatcc aatatttgac tttactgctg tatctctcta atactgtctg
    64561 agcaaatcgt tcatagaagt cgatcagttt tcgatttttc cagccaccgt attcttttgc
    64621 caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca ggttcaatat catattttaa
    64681 gcactcatca atcagctgat cataaaattc taatcccgct tcgttaggag taccttcgtc
    64741 tccatttgga aaaattcgtg tccaggcaat cgaaaaacga taacatttga atcccatttc
    64801 cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga tcaattcctc gatgattcgg
    64861 gtaaatatat ttttcttgat caatcgtcca gtcaaattct tcactgccga taatcgcaaa
    64921 gcgctgctta cctccaggca tagcatctgc aacggatagc ccttttccgt ctcgatcata
    64981 agccccttct aattgattgg cagcagttgc tccaccccat aaaaattttt ctgaaaaacg
    65041 tgattgcatc ctgatttcct ccttatttta caattgccag aacatcttcg tttgcaataa
    65101 tctctttttg gtctaattca agtatatcgg caaatgcatc agtattcgta atgacaactg
    65161 gagtgattac gctatagcca gctgctctaa ttgcttcgat gtcaaaacga atcaataaat
    65221 ctcctgcttt gacgagatcc ccttgtttga ctaagatctc aaagccattc ccatttaatt
    65281 ctactgtatc catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta
    65341 cagcatgtcc agttggaaaa atagtcgtga tctctccgtc tgcaggtgca actagttctc
    65401 ctacttctgg atcaatagcg ataccttttc ccatcacgcc agaagaaaaa acttggtctg
    65461 gtacttcact caatggcagt actttacccg ttaaaggact ttttaaggta tgccgtgctg
    65521 aagtaatcgg ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt
    65581 cgtcaaatcc taaaatcaat gtacctacga atgcaagaac aaaagcaatt cctgtagcta
    65641 ttactgctac tgtcacattt gactctacac catccaccgt attaatgaaa gtcggaatcg
    65701 tcaataagct gaccaaactg ctggagaaag cttttactcc ggaaaatccg ataatagctc
    65761 caccaatacc tccagaaata caagcggcaa tgaatggttt cttcaaaggt aatgtcacac
    65821 catacacagt tggttcagtg attccaaata acgaagtcac tgtagaagaa atccctaatg
    65881 ctcgaagttt cgtatctttt gttcgcaatg ctacagctaa agcagcccca ccttgtgcca
    65941 agatggctgg aagaagcatt ggcatcaata catcaaaccc atattgttcg atattcaaaa
    66001 acatgatcgg tacgaatccc caatgcatgc cgaacatcac gaatacttgc cataaacttc
    66061 ccatgattag acctgctacg attggactaa atccataaat cgagttgaag agaccgccta
    66121 aagcattacc ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat ggaaccatca
    66181 ctactaaaac gacaagcgga actaaaatca tttgcagaaa ttgcggaatc acttttttta
    66241 cgaatggctc taatttactt tgaacccata cggctagaat gattggtatg acagaagaag
    66301 tataaccaga tggtgaaaga atcactggga tcccaaagaa actgatagct tctcccgcac
    66361 cagcaactgc tgtgatagct ggatacacaa gtgccatagc aattgctact gctaaaaact
    66421 ggttggcttt gaacttttta gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga
    66481 caccatctgc tgcagcaaac aatatttgat aagtaccgct atcagctgtc agccagccca
    66541 tcaccgttgc caatgataag aatcctttca ataccccggc ggctgccatc gcccctagaa
    66601 aaggtgtaaa tatactagag ataatatcga tcagacgatt caaaatattt ttggactctt
    66661 cttttccatc ctccgaaact gcaagacctt cctcatccac gatcgattga taaacatctg
    66721 ctacattact gccaatcact acttgatact gaccgccact ttgtacgact tggatcacgt
    66781 ccgggtcttc ttttaacttt tgcgtatctg ctaaagattc atcttttaat ctgaatcgca
    66841 accgcgtagc acaatgtact aaacttttga cattcttttg tcctcctacc gcttcccata
    66901 cacgacgacc aactgcttga ttatccattg ttttttcctt ctttcttttt tatttaaaaa
    66961 ataaaaaccc agattgaaaa aaacagtcac aaacattcgt gaacctattt tttcaatcca
    67021 ggttttgcct gccatacagt aacaatcctt atgattggat gctttttttc tcacttacta
    67081 ctctttgaat atgaatcgtt aaataaatct gctctgcgat ccccatttcc tgtttttgcg
    67141 tttgtcgaag atactcattg atccgttcga cacaatgaaa cgcttttgga tactttcctt
    67201 gaactaatgc gtagagaaat tcatcttgct cgtcagatcc ttcccctttg agataacgct
    67261 gggcaaaaaa ctgcagatga gtaatgatac gctgataatt caaagattct tcatcgaaca
    67321 cgatgccgaa gaatttactg atgatactca gtatatctct taccattgtg gtagcggtca
    67381 tcgtcacttg catgtcttcg tttccttgtt ggctattcac aaaatgaaag gcaataaaac
    67441 cggcttcatc ctcagaaagt tccacacccg tcttttgttt cacgaacaaa agagcctctt
    67501 tcgctgcttg atattctttc ggataaaatt tttttgtttc aaataataat ggattaggca
    67561 gttggatatt ttcttttgct cgtgtgatcg cataatgaag atgatcggtc aatgtcagat
    67621 aaatattagg cgaaaattca acttttagtt tatcttcagc cagttcgact acttgtttga
    67681 cccattcgat ctcctctgcc ggaatatcag ccatcagctg ctggaattgg tttttagcaa
    67741 aagaatctct taaaacaaat tctttttgga ccgcttctgg cgaaatctcg tccccaatct
    67801 ttttttgaaa agctagtcct tttcccatac aaacgacttc ttccccgaat ccatttttcg
    67861 aaatgacgac attgttattc aaaattttat caattttcat tttgcttcgc cctctcaaga
    67921 aaaataaaaa aacctagaat tcgcctactg tttcttagct gaattctggt tttgcctgcc
    67981 gtacagtaac aatccatttc ttattcaaaa tcatcatacc atcgaatttt tatttatgca
    68041 aaccttttca tataaaaaat aaaaaaactg gaacacaagt ctatctagaa aacaaccaaa
    68101 tcagagatgc acctctttgc gtttctcttt ttttcagcaa attggttttt ccttatcgat
    68161 tcatatcctt tggctgtttt taatctgtaa cgacttatgt cccagtatcc taaaatgttt
    68221 ttgtctctag attgaaagca gatctcatat catctgtcgc atcttttatc gatgtaacac
    68281 tttccagttc tccttgtaca actagtcgcg tgacggcgtc catgtcgcca caagtaatta
    68341 gtgtgacaat ttcttttccc tcgacttcat tcagtaattg ggtatctgta ggagcaactt
    68401 tttctttaaa aaatgttttg tatgtataca cattttctaa atctgtcaaa tagatcttgg
    68461 cacccatctc taaattttct agcggtgtaa acagcaattc agggttcgtt gcacgatggc
    68521 ttgctaaggc ataatttcct tttcccattt cttgatctgg agataatgtt cctgctccgt
    68581 aaagtagtga cgtattatcc aagcctttga aaaccggtaa attgattgaa actgaaggga
    68641 ctgcgatact agcaatcact ggtagatctg ttcctctcat ttgcgacctc atgacccctt
    68701 ctgtcgtcat tggttctacc gcatcaaagt caaaactgac atctttatcg ttattctttt
    68761 tcagatcttc ttttgttaca ttcgctatag catatttatc tcctgtctct ctgacaaaat
    68821 aatccttgat ctgttcattg aaaatcaaag ctagtcctac taataacaag aggaacaaga
    68881 ggatattgac tagccatctt tttgcttttc cttttttttt cattttctct ccctccagac
    68941 tcattttact gtttttcttt tgctctttcc aacagccgaa tgacacattt ctacaactct
    69001 gtgtattttt ttcgtacacc tgcgcttttt tctacaggat atttcaaatt atttttgatc
    69061 aatttttctt caatgatctc atctagatcc aggtgaaggt tgtctgccat catgtatgaa
    69121 tagatcaata catctgccag ttcttctttg attcgttcta attgtgtttc ttttacttct
    69181 tccggcgttt tccattgaaa aagttctaac aattcagatg cttccaaaga aatagagata
    69241 gccaaatctt tttcgttatg aaattgtcgc caattacgtt catctctaaa ctggttgact
    69301 ttttccatac tattcatttg cttgccttct tccaatcact tcttacttga ttaatcatac
    69361 cattttttta cataaaaaat agatgatgct tttaaaaaaa ctcctgtttt cttttattta
    69421 aaataaatgt catagaaaat caataattta ggtagccacc atgtgtatgc agtagcgtgt
    69481 cttcttccct tgaggctttt tgtttttcga tacgttcttc ttcccaaaca gacaacgcta
    69541 ctagaatctc tcttaatgat tttccttctt cggtgagcga gtactctact ttaggcggga
    69601 cttggttata ggcttttcga tggatacttt catcttgttc taactctctt aactgctggg
    69661 tcagcatttt ttttgtgatc gtagggattt gcttgcttct ccacgcctaa aataagcgat
    69721 attgctcttc cctttatctg gtgtaagcaa agcagttagc attttgacga tcgtgggttc
    69781 cgcttttcac ttcttatcta cttagtatca tgaagtatac tttcgtacta atactaagta
    69841 gcggaagtgt caactacttt gtttaactac atagcaaaag aaacagaagg gtgacaacgg
    69901 tcatcacgct tctgtttctt ttgaatatgc tgtgttgttg tctctatctc cgtgtagact
    69961 ttcctcaaac ttatcctttg acagtaagtc agtaggctga tttgctcttt ctagatgcca
    70021 gatacatttc tacaaaaaaa tagtgattct gttttgaaaa ttcgattttt agttctgcat
    70081 tttcgtaaac atccaacatt tttttcgcac tgtatagacc taacccgcca ttttttttag
    70141 aggtttttcc tcttttgacc atctccgata gcgtcaaatt cgttggattt gccttgttcg
    70201 ctatactaaa acgtgatccc tctttttctt ccagcagatt gatcgtaatc ggactctgat
    70261 cggctacacg atgatcgaat gcgttagaaa taaaaataga taaacagcga ataaaaacaa
    70321 ccagatcaat cgggattgtt ttcacttcga caggaataac tacttggatc gggattttct
    70381 ccgattctgc tttttctaga aattttaaaa agacacccct gacagctggg gatacaatat
    70441 ttgttagctg ttgtatttgc ggtttttcaa aaatagctgc agaatgatct tctaatattt
    70501 ctaaaaattc caacgtttct tttttatttt cctgaagagt aatttttaat gaaaacagta
    70561 gacttttata atcatgtttg aattcctcta gatgatggta atattcttca tttatttgat
    70621 acagttgaag cgattcctga tgttgtcttt cttttacttg gaaactgatc aacataatgg
    70681 aaaaaaacaa agaaaaaaaa ctgatgacta tcgacaaaag agatagaagc agataattag
    70741 atgagcggaa gtgaacactt cccaatacca agtaaatcac gccccagctt aataaaatac
    70801 aaatacctcc aaaatagtgt tcagttggat aagtaaaaag caagttgaga aatctcgtct
    70861 gtttgtctat actttttact aatagataga aaataaatgc gataagtagt gcaaaaaaac
    70921 ttgctgttcc aagatgtctc tttcctaaat aatccatcag atcaaaaacc agaaaaaagc
    70981 tgagtccagt gatagcaaaa aaaagattac tgatcaaaca gctgataaac aggtttttcg
    71041 tagtaaaata aaaaagaaac atcaataata agccaattag gtaaggaaag ctcactttag
    71101 ggataggaag taaaagtgtc accaccatca aagctataca accaaagctg ttttttacta
    71161 gcagttgttt cgaaaagaat aaatagatac ctaaatctaa aatcaaagta aaggtcaaat
    71221 aaagtgtcac tgttgcgacc atcttattcc tccattgttt tgtttcaaaa tgtacacata
    71281 tttttttaat aggagcctgc atatgaaaaa gtattttatc atagaagata accagtttca
    71341 cagcgaatgg atcatcagag aattggtcaa tcatcatatc gagaatatcg tgcagctcac
    71401 ttctatctct caaatcaatc agttgatcca gttgggcact attgataatc acgatgtttt
    71461 ctttatcgat atcgacttga agcaatcact cacaggtatt gatgtcgccg aacaaatacg
    71521 taaaaaaaat gcacatagta atattgtttt tttcaccgct tatacgaatt ttgcagtaga
    71581 gatcatcaat cggaaaatcc ttccgattgg atatatcaga aaagacagga atttatctga
    71641 acaaattcgt caagtgctag aacagatcaa ttggaaagaa cagcagatcg taaatgtcga
    71701 taaaatcttt attgatcaaa aaggtggaag tctcgtcctg tatcctcggg aaattttata
    71761 tattgaaagt atcaaaggtt tgaaaaacca agttatggtc aaaacaacta cagaagataa
    71821 attagtgaat acttctttaa aacaattgaa gacagagtta gaaaagtatg cttacttttt
    71881 acttttaaaa tcttactgta tcaacttatc gcaattgcag aaaattgatt ctgctcatta
    71941 tgtgttagaa tttttcaacg gtgactcctt actcgttggt cgaaagatct ttgaaaagac
    72001 aaaggaacgt ttccatgact tccaaaaaac tgcttcgagt tgatgaataa gagctgagac
    72061 gtaagcatag cagatttcag ggagtgggcc ttgaatcggc tccactccct gaagtcgttg
    72121 atatgatttt acgcttcctc ttctgtagga gcatctgtta acgaccatgt gaatgttgaa
    72181 acgtagttgt ctacagcata attggataca ggaacaaata attccactga ttgtctccct
    72241 tgttccacat tctctcctaa tgaataaatc catgtgccaa tcccttcatt tgctcctgca
    72301 gttactagat ctactgcctg atctgctggg atttcgattc cttcagcgcc agttccagaa
    72361 acggtagatg gcattccact gctattagtt gcgctatttc gaatcacggc gtttttgaat
    72421 ttcacctttg aaccagttag tttattcgta tcagatgttg taccaataaa ttctgttcgt
    72481 ttgactgata atttccaacc ggcataattt ccgcttttat ccgttacttg tacgaaattc
    72541 tgtacttgtg tttgtgctcc agtactgtcc gttccgcttt gtaatgcagc atatactgtt
    72601 tttgctgtac ctaacttgat tgttccaaaa tcaagatctg gagcttggtc aatcgtcagt
    72661 aatccggatg ttccctgatt tggttcgtca ggtgtaaaag gagtatcagg atcatttgga
    72721 tcaacaggag gaactggaac aacgtcttca ggatttgcag catctggttt ggtcacagaa
    72781 atcgttcctt ctgtagtggc tgagctctta ttctcagcaa aaacactttg tgtacataat
    72841 aatagacttc cagctagaac ggtacttaat gcaattgttt ttttcatttc tttttccttc
    72901 tttcttttaa aataaataat ccagcaaata agccaataag aatgactcca acaagtatca
    72961 gccatcgcca gtcaaatgtt tctggctgat tttttaaatc aatatccttt tcattcaatt
    73021 ggtccgctgc atcatcagtt atttcaaaat cggtatggag attccagcgt ttttctttcc
    73081 aagcaccctt cacaaccgct tgataatgtc ccggagctaa tttttttcct tctaatggga
    73141 caagtaaatc aaaatttgta ttgggtgcta catctatttt ttcttttttt attgaaaggt
    73201 aaggctcttt tgtttctttt ttgtagattt caaccgatgt agtcaagccg aacatataag
    73261 aagcatttgg attttgaaga ttcgccttaa tcacatttct tccatcgctt tgatccgcat
    73321 aagctttttt taatttcaaa tctggggaag actctttttc gctatttcaa atcaagattg
    73381 ctttcacttg agaaaactga ttgacgacac caactgtact tttgttagaa acgttctctt
    73441 ttttttcttg aaacgtgatc cctcctgcga ccacaccagc cagctctttt tctggcgctt
    73501 tcagtgaaag tgtctcagtg accgcacttt gaggaggaac agttatttct cccactgttt
    73561 ttgctaagtt tttcatatca taacttaatg tttcatcaaa tttacttttt gattctccat
    73621 attcgatcac accgtttgaa ttcgtcgtag cggagtggat caatgtctca actgttactg
    73681 atttatctgt tcggttaaag accgtgtagg ggatttcctg tgttttccca ggctccatcc
    73741 ttacatttat atatgttttg ttcttatcaa tctgattagc cggtaattca aaatctaccg
    73801 caaaattgaa ttcggaggca tggacaggtg tctgccaaag gatgaatcca aggagaagga
    73861 tcataagtaa agagcagttg tttcgatgac ttttattcca tattcgattc atcaatcatc
    73921 ctccttgatc tttattctgt cggcgcatct gctaatgtcc atgttatctc tgctgagtaa
    73981 ttaccagcta tctgattttc tttggttaag acaagtttaa acgcccgacc gttatttaaa
    74041 tattctgaat aatctgcttg cacattcgtt gaattttgtc ctctgacaat ttccagttct
    74101 tcttcattca ctaattgata ctcctctcct ttcttgaaat ataggacatc ctgcatagaa
    74161 aatcctgcat catttgtgaa aggctgagat aattttatcg ttaagatcca cggatttttc
    74221 gtttctcgct gatcataaat ccataatttt tcctgtatat ccatttggcc aaatgcttct
    74281 tgtgtcaaac cagttgcttt gatctgacca aaatcgagtt tggaaggggc aagaaatgag
    74341 agaccacctt ctactacgat cttcgtagca ggtgcaaaag gcggggcatc atgataaaca
    74401 actggcgtat cacttggatt gtgattccca atttcatcgt tggtacttgg cttgttctgc
    74461 acacccttag cagtagcttc tcctgcttgg tcgtttaata caatttctaa tacatctcct
    74521 gcaaaaagtt gattttctgg gatagaaaga acaaattccc ctgtatcatc aatagttgtt
    74581 tctgacaaaa cagcttgatt ttttttcaat gaaaccaagg ctcctgcttc acctgtacct
    74641 gtgatcgtgc gagaagctgc attgatcaat gtggtactta aattagcagg ttcaggagga
    74701 atcacatcga ctgtcgtttt ggcagagtcc agttctgtca cttcttggcc acctcttact
    74761 tttttagcag caacaacttg cacttgatcc tccgttttta atctctcatc attgggtact
    74821 tgaacttcga acaagccgcc ttgttcgcct tctccccatg ggttatatcc tgccccttca
    74881 ttttcatctg tttgtccttg cgtgagagca gtaatttcta cagatgattt cgtcgtatct
    74941 gcgtaagtaa tacgtaaagt cacttctact tcgcctgtcc aagcatctct tggctttgcc
    75001 tcgacacctt cgggaacaac gacatgccca tagatttttt tatctgcatt cgttggcact
    75061 cttaaggatt caatcatcgg gagctgatta ttggcactga ttcgattata atttttcatt
    75121 ccggcacctg tctgagcaaa ttcatttgtt aaacctgcac tagggcttga tacgaaattg
    75181 cctaagtcgg tcccattgat cgataagtct gtcatatcag aaaaataatc cgggtctttt
    75241 tcaaagttat cgaatgttgc gcctgttttt cgccataaag cgatttctga atgggcgatc
    75301 tttaaagaag aatctccatt tccttggaat acccagccgc ctatgccatt ggtctcgctt
    75361 ccaatccgtt tgttttggaa atcaaaatag gtaggagatt ctaagtccac attcaacaca
    75421 tccccactga tgatgccggt cttatcttcc cctgttttcc cgaccatgac taaataagtt
    75481 ttttcacctg ccgtaaaatt taaatcaata tctccagttg tatctacagt tgctccggaa
    75541 tctgcgcgta tatcgatatt tgatttttct ccagtgactg taaaatcagc tgaacctgga
    75601 ctttctagaa aaccagaagc gtcataaaat tggatcgctt gatttccttg attgagtctg
    75661 ttcacgtaag atttgccatc acctgtattt cttaccctca atgagcctcc attggttact
    75721 gtcagctttt gcgtaccatt ctcaaaacga actgcgctgg aacgcccact ctgcttatcg
    75781 atgtcgataa ctgatccggc tccttctacg atcaaagaaa ggccttttcc tacaaaacgc
    75841 atcccattgt tcccgggttg atcgtcatct ccagccattt tgaccgagat ttgtccacca
    75901 cttgatacat ggatttgact agaatctcct tgcaagcgaa atccgcccgt agttgctgta
    75961 ttgatggtga ttttcccacc attcgataca gtgactttcg atcctgaacc tctcatttgg
    76021 atgattccgc gattgttgtc attggtaata ttgatctttg tatttaccga aaattcgctt
    76081 cccgaaccac tgacttcaaa catgacatta ccaggtagcg tatcgactgc ctctccttca
    76141 tcgctaacgc catctgcccg atcataatca agagagacct ttgaaccgct taagatatgg
    76201 attcctggcg ctgtttttcc actaccagtc tcatctcctg aatctagaaa accgtagaat
    76261 ttccctagat acggtccata aagttgacta ccattgatca cattcaattg atagcttcta
    76321 caaaaatagg ctttacccgg agcggcattc agtccttcct tcactggttg gacatccaac
    76381 acaccggaaa caccatctaa tgtgacagtt gcacgaggag cgtaaatcag tcctaatttg
    76441 gacccatcat acaaatcaag tgtcccggta aattttattt ctcccgtaaa atgatatttg
    76501 cttccccaaa aatcggtcat ataagcaatg ataatggcat ttccatcgat atttggtgtc
    76561 aatccacctg gatgtgcagc tgttccttta aaattctgga actcgatcga ctgaggtatc
    76621 tcattgatat aaatatggtg tgtgccgaaa ttgtgagcga atccaccaaa atcaataacg
    76681 atatctcgtt tgatctctaa ttccctgtct aaagtcaaag gctgaatcag tcggatctta
    76741 gttactgatt cgtctgcgag aagctgcgcc agttcttctg ttgtggcagc ttcgctgtac
    76801 ccatccgctg aaacagctct tgccagtgcc gcttcactgc tatcagacgt ttgttcagct
    76861 acttctgagg tctcacttgt cttattttct tcttgccttt tattagtaga agcctcactt
    76921 tgagattctg tagtttcaga agtaatcgat tcttctgaaa aagtagtctt gtcaaaactt
    76981 tgattctctg attgcgttga ttcgtttgtt ttctctacat cggcaaaaac ggatgtcacc
    77041 atcaagagag ggaagacaaa caaaatcgac gcaatcagct tttttacaca ttgattcatt
    77101 ctccaccatc ctctctgacg ttttgacaat tttattttat cgttttaatc tatttccaat
    77161 tgtactttca tatttaaaat atgtattttt atggaaaaat ttttctatta ggtgtaaatt
    77221 tcttttttgt acaaaaaaaa ggctgtgaca aaagtcttgt cacagtccct tatcccgaac
    77281 aagcggtgtt ctcccgccaa ctcctcggtc acaacctcca ttttaacaat aaacctgtaa
    77341 atgtgagggc aatactttga ttgtcacagg taatttatcc ccttcatctc catcaacatt
    77401 cgtcgtcaat tcttcctcat cagctaatga cacaactaat tgctcacaag taaggtaggc
    77461 tagatttttc gtagattcat ctactccttt taataggtct ggcaatgttt taagcgtatc
    77521 gatcaaagaa gaatctttta aataaaccaa atggagctta ccatcatcca cttttgcatt
    77581 tggaagtaat gtttcaaaac cgccgactga gttagttagt cctatcagca aggtactgct
    77641 ctcaatttct tccttctttc catcaacttc tacgtgaaac gagtaggatt gtgtggaagc
    77701 taactgtttg attcctgaca tgaaataagc tagttttcca aatttcgtct tcttctccgc
    77761 atctacatca ttgattgatt cagggataga gccaattgcg acgacattca taaaatagtc
    77821 gtcatttatt tttccaatat ccaatggctt gactgattca atcgaaaaat gattgatcgc
    77881 ttcctcaggt tctagcggta tccctaatgc tcgagccaag tcattcaccg tacccaatgg
    77941 gaaaaatccg aaatttggcc gatgttcttg ttcagcgatt ccactgattc cttcgttgac
    78001 tgttccatct ccgcccatga caaacacact atgataccct tccgtagccg cttcgcgagt
    78061 aaagtttttt gcatcccctg ctttttttgt atgcaagacg accacttcat caaacaccga
    78121 ttccaatttt gctatcgcta attgctcaaa ttcctttgct tgttcaccgc ctgaactggg
    78181 gtttaccact aataaaactt ttttcatcac tttctttccc ttctgattga tttctttaat
    78241 ataacgatag cataaagaaa aaaattaggc tgtgatgaag tactgttcat gaaacattca
    78301 tttatttttt gacaagataa aacatccata ctatgaaact gaggaaaatt aaaacaatga
    78361 tataggaaat aaaagaaaat gataggcatt gtatacctaa taaagtaaaa atcttaggtg
    78421 gcagataaaa acacaaaaat agcaagagaa tgtaatagcg tttcttccac tttttgttat
    78481 cagaccttat agcgatcaga ggaataccaa atgcataact gaaaaactct tgatgtactg
    78541 ttaatatgtc acggccttga ataataaatg cgacaatccc cgctataatt agtgtgccta
    78601 acaccaaaac ggaaaattga tgaatatatt ttatatacaa atgatccatt gtacttcctg
    78661 cctttttatt tgtgagaaaa acaaatccgt atacgtgaat aacttctaga aataaaagaa
    78721 atttgcaaaa ccactccatt tatctacttc tacgtgcatt ttttgatatt gttttcttta
    78781 tttaagatta tacagaaaaa tatctagggt cgctggattc taagtaaaaa aaatttttaa
    78841 aataaaaaga actaaatcaa gtagtaatta aactactgtt gatttagttc taataaaaag
    78901 atttagatga acagttttat tgatttaaat agttcatatt ttttatttat accgcttatt
    78961 tactacttgc tgtactagaa tctgaagtga atgtactcaa aacattttca aagtctttgt
    79021 ctttgatttt tacattagcg tctttcaata cttcgccaat tactttattc tggaaggtac
    79081 tgtcacttaa ttttgtgtct gttgcaattt ctttcaattc ttttttgtat ttatccatgt
    79141 cgttgccttt gttttggttt ttcaccattt taaccacata atattccgta gtataagttg
    79201 aagcattggt agaagtgatc acatcagaaa cttgaccatc tttcaactta aaggctgctt
    79261 ctttgacttc tgccggaacc gttgtagtag tggaatcaaa tttcactttt ccgccatctt
    79321 cttttgtcgt agtatcagtt gacttgtctt tggctaattt agagaaatcg tctcccttat
    79381 cagcagcttt cttcacatct tttgcatcat cttcacttgc tacttggata atttgagctt
    79441 ccacttctgg atggaaagat tcccaagccg tttttaaatc ttcatcggtc aatttgatat
    79501 gtttctttag accttcttga aaggctagac tttgtttgat actgtctttg aatgtttgtt
    79561 ctgtgtaacc tgcagatttc aattgtgaat caaatgaatc acccaattgt ttctttgttt
    79621 gatcatattg agaatcgatt tctttggttg atacgtcatc tccatattta tcttcaaata
    79681 cttgataaac gatcaactgt tgcacagcgg aatctgctcc ttgcattttt ttcaagctat
    79741 ccattacttc ttgttcgctg attgtaccgc ctttataact aacggctgtt ttttcaccgg
    79801 cattcgtaca gcctgacaat aagccagtgc ctagtacgat ggcaagtgct aaaaatcttt
    79861 ttttcattta tcaattctcc tacttttcgt tttttgcgaa ttgctaaaaa caaaggtgtt
    79921 caagatgaaa atcatcaaga acacctttac taatctgctc gatacgtcct gaatagatat
    79981 aaggctacac aatccagaaa gttcgctcag cagaataaat gtcaagtagc acatcacacc
    80041 ttaatatact acttcttttg ttaccgtttt tcaagtctat cttgcgttcc gtatttaacg
    80101 taactaattc ttagaacaaa tgttatcgat tttgtttttc ttcttccgct gctgggatat
    80161 tcaaatcaga cacttttatg ttcgattttc cagacatttt tttgttgttt ttattatgat
    80221 atatttttat acaactaaaa atgaatatat taaaactacc aaaaataact tttaacgctg
    80281 tttcattata tgttttttct attttatata aacttttgga tacttaatac caaccggttg
    80341 gattgtacca acatgagaat gtgtcagttc tatttcctgt cgcacttgca taggcccata
    80401 ctccttttgt tgcatttgct gatcgaatag agtttccaac tttcgctttt gcaccgtgac
    80461 gattatagac acgactatag tggttagaat gacctgtttt tctcgtacaa gttgtatcac
    80521 tataataaac ccatcttcca gagctatgat taattgctgt tctagaaatt tcccggtcta
    80581 ctggtaaatt tgacttaata atattacctt tagcatcaaa atgaatctct cctggcttta
    80641 aagatttgta atagatttca ccgtgaactg aatttgcttc cacttcagat gacatagcgt
    80701 ttcctccaac aaaacctatc cctagtgcac aaataataac tgcttttttc ataaaacgac
    80761 atcctttcct ttttgatttt taaagcatga tcttcttgtt tcctattttg gtcagatatg
    80821 gatcatgcgt gactacgata actaacgtac cttgggaagc ttgttgcgat aagatttcaa
    80881 ctactttatc cttatttctt ttatccaatg acccagtcgg ttcatcggct aaaatgattt
    80941 tgctattctt caataagact ctcgcacagg cgattctttg cttctcacca ccagataatg
    81001 tgtagatagg cgtacttagc ttcttgttta tatttacttt tttcaatgcc tcatactttg
    81061 ctgttcgctt atctgaaaca tttaaattcg aaaacttcaa tccaatatct aaattaaaat
    81121 cgattgtttc atattcaatc aacccaaaat cttgaaaaag aaaagatatg gtatttcttt
    81181 ttatcaagcg cttctctctc ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt
    81241 gtcctgaact gaactgatca ttcaatccta atatattcaa taacgtactt ttacctgctc
    81301 cactttctcc aacaacagtc acaagatccc cagcatccgc agtgaaacga tccaaatgaa
    81361 gaatcgtttt gtttcgaatg atttttttcg caccttttat cactatcaac ggctccaatt
    81421 gttaacctcc attcatcagc aataactgac ttttcttttc tgtagacaaa ataaaaacaa
    81481 ctgaaagaat aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat ttaggctctc
    81541 tttctataag aaaaaggaag attacacttg tcgttcccca tttgatgagg ttcatcatga
    81601 agaaatcttt atacgtttca aagagcgaat aaccacttga acgaaagata gcgattttcc
    81661 gacaattgta gacaaaatat atgcataaca tgacatagct caacaagaag aaagaaaaga
    81721 aagtgagtaa aaatatctgt atcaagtaat gaaatgatct gcttaacact ttgacagcta
    81781 tctcttcagt ctgagtatac ggaaatgata actgcaagtt atcacgtaac tgactttctt
    81841 caatcagctc aaataacctt tctttcgaca aagagcctat cttgatcttt aacggtggat
    81901 agatttctcc gctgagaata tttctttcca aatacatact gtttttcaat gtcagtattt
    81961 caacaaccgg ataatgttcg atccagggga tagaaggaat aaatgtataa atgggttgat
    82021 tgttttttat aaatagaaat ttcggttctt tttcactgat ttcttgataa tattctgtta
    82081 tttgcgccag gtcatttttt aaatctgtat gtctatatcg ttcagggatc aatataacag
    82141 ggtgcgtttc tttttcggtt atttggatca accttccaga gacatcaatc aaaggaaatt
    82201 tcttaagata attcagattt atttttatcc ctctgccaaa ctgttcattt tcttcattca
    82261 agtaatctga tacattgact aatatacttc cgtttttatt caaagtttca tataattcct
    82321 gatctgctgc tgatgcaaaa cgattggaat aaataaaatc cacgtggttt ttcccgatgg
    82381 ttactggata aaatacatga tagtcctcac cagcgatttc ttttggtggt tcatgggaaa
    82441 cagctaactg tgaagaagaa aaaacgatag ttgctaattc gacattggca acggtcaaaa
    82501 gaagagaaaa caacaatcca aatgtaaaaa gaatgtattg gatatagttg aacgcttctc
    82561 ttaacgaagc gattttttca actaataaaa gagaaatcaa agagatacca tatattccgc
    82621 tgaacaagaa aacgtacatc tggaacaaat aaagaaattg ctccttataa tacataaaaa
    82681 taaaaccacc aatgattcct gcaattatga atgtaataat gattttcttt ttccccaata
    82741 aacaccacaa tacttgatgt aatgaaaagt cattattcaa caataaactg atttctttat
    82801 tcatcaataa gaaaaaggat gataacaaaa ggaataaaag aatcaatcca atagttaaat
    82861 acactcgata attatatacg tctgtatttg aaataaccga atacgaatag ctattttcaa
    82921 agtcttcaaa aatattagtg gcataatcag tgtgaaatgc ttgattgaat cgatcagtga
    82981 atagtgaaat gacccgttca taggcttctt tttctctcgt catcaagaaa aattcacctt
    83041 caaaatcttt tattttgatc gattcttcaa tggacgttac gcgaaaggag gtattagaat
    83101 agggtacctt ctcgtgagaa gatttgacat ccccttgata atacaatgtc atttcaagct
    83161 ttggctgtat cttgaagttc caagatgtat ctacttcatt ggattcattt actgtttttt
    83221 tcatataaga aagctcagtt tctgatgaga cattttcaaa taccatgatt agctgctcat
    83281 attcttcttg cccaataaaa tgttgtggca atcgaatcgg agtataagta tctgtttgat
    83341 ggaaaaattc atttgtccga tagagtacct gtttatttat actaaccaag gcaaaaccac
    83401 tcatgatacc ggtcactatg attgtgagta aaactgtgaa cttttttttg attatcacca
    83461 ctctccctta cttaaatgat tctttgataa aatcataccg tttttcatca aaaaaacgta
    83521 tctatcaagt gtataggtca tgtagaataa atctatatac tgtagaatcg aaaggagaag
    83581 aagatgaaca ccggagaaaa aatacgaact taccgcagaa attgtaagct tacgcaaaaa
    83641 gaattaggcg aaaaaattgg tgtttccgat aaaacgattt ctagttggga aaattctcgt
    83701 acaatgccag actttgaaat gctgtcactt ttacatcata ctttaggtct gccgattgat
    83761 tttccttcta ttgaatcagc taccgaatca atagaaccaa cattgccaga tgaacctctt
    83821 gttaacaaga aatcttattc atcaaaacaa cccttctcac tcaaaaagac actatctttc
    83881 tttctgctcg tcttttgttt ggtctatctt tttcttatca attatcaata caaaaatgaa
    83941 acgatcgatc gattcaatcc ttttttgaaa ccacaaatcg gctataccac actccctgta
    84001 ggatacgtgg acaattccat cgaacgttgg gtatgtaatg atatctttgg ccatggtaca
    84061 tggctgtctt ttcacgctgg agaggtagaa aaagagaaaa accatgctgt aatcaaacac
    84121 aaaggaaaac acgtcatgaa cgttcgcctg ctcacttggg aagccattcc ctcagattac
    84181 caagaaatcc tcagccaaga ctatgacgac gccttaaaaa gaagccctca ggagccagat
    84241 aggcattaag taggagatga acgtagagat agaaaaaatc aatgcacatg atcttatcca
    84301 aaaaacaggg gtacaaatta attgaaaaag ccttgagatg aaagttccta aataaaaaag
    84361 ttcagaatga taggtatttc tggcttatag tataatattt acgccacagc ctcccaatga
    84421 ttctttttgg aatcataatc aaggatggac attcacaaaa atattttgct cattcgatcc
    84481 gaatagatta acgaccaatc tagtaagtaa tatagattgg tacgtagtca agctgcacat
    84541 aatttagatc aattttagtt cttttttata tttctatgat ctgctcattt acttttatat
    84601 gatggcttac agtaacataa ataaatatga taatcgctgg tagtttatcc aagagtactc
    84661 aataaaatac aaaatggatt gttcaagcgc ctatcataat gataaaatga aacaaataaa
    84721 cgatacaagg aggaagagac tttgagtaat ggcagaacaa ttttttcatt gtaaaaattg
    84781 ttcagttgtt aatctatggg atttcggtag catgggcatt tttttataga ggaatcacac
    84841 caacctggcc gttttatctc ttgttagtgg gaatattagt aggaaatttt gcccctcttc
    84901 actatcgtac gaactttttt ccaactgatt ctggcctttt tcttaaaaca aagacgcgaa
    84961 aattagaaaa cattattgaa atgacaatta cctgtataat cattttctta gttatattcc
    85021 taaacgtctt ctaataagaa tctcttaaaa ttagcttgtt acagttagtt acctagttta
    85081 tataacacag ttatcttatc ttccttatca acatcttttg ccaactcttt gtcttaaatt
    85141 cgaaacatat aagcagagct acgaacacca agactgtttc ggctctgttt tttgcatcaa
    85201 attcactctt atatagctga taatctcggc tctttaaaag aaattatttt cgcttcattt
    85261 tattataaaa aggctaggaa ccttgatgtt atcaggttcc tagcctttat ttaagtatta
    85321 ccccacagac ccttccatat agatttttga attacacact agtttaaact aaaacaaaca
    85381 aggaaaaact taccatttaa tcataaaatt agctcatatg aatatattag aatgtaaaat
    85441 agactggtca aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat
    85501 tttgatattc taaatcattt tttattggat tattcccttg ttcgatgtag actcaaatca
    85561 atctctttac ttgctaacat cctttatttt accttgacct gttgataata gacagttaaa
    85621 tcttgattta aagtggggca attaatcaag aaaacagggt tctaagcctc atttgttagc
    85681 ttgttctctt agtaatattc ctcgtgaaga atcgggacga ttgtttggtc aataaacaat
    85741 atctcacttg catcgaaaga atatttcttg aacaactgca tatcaacttc ttcaatacct
    85801 acattccagt taatgtctga acctgaggta aaatcttgta ctggtatgaa catgaactta
    85861 tctttggata ggtgaataga tgtcactgct tgaagtaata aaaatctgta aaaacgtgtt
    85921 cgtaaatact tcagataatt aattgcttcg gtttcggtat cagcagaaaa tagcactagg
    85981 tatgaatctg taactacttc attaggttta ataactctgt tagtagaaat caccttaaac
    86041 ataccatttt tgttcggttc acctgcatgt tcgctagtca gtgagctact aatgacttta
    86101 tactttccaa ccatatcctg tcctttagtg atatcttcca gtggaacaaa gcctacccca
    86161 ccactagaga ttacttttat tgaatttctg gaatcctgca cttcttcccc tcgaattttc
    86221 gttggaagcc caaatgggtt acgtgatata acaaagtcac caacactagc gtgttcccca
    86281 attttgtgaa ttgcagaaat ggcttcatta tatcgcacaa acactggaaa ctcgttcatt
    86341 tttcgctctt gtgtatttga tacaccatca tgttgattag tatactgtgt caatccatta
    86401 tatttcttat cccacaagaa atagctaaca ccgccaccaa tactggtgtt tgggaatatt
    86461 tctttcccat ttactatatc caccatcacc ttcaagctag agctgttaag cataaagtca
    86521 cggtacttat ctaacccttt accacctgca aaccacttcg aaggagtaat catcgaaaca
    86581 tagcgttctg ataacttaac agaggtttct acaaatagat tatatagcgg tgtagcacta
    86641 gctcctgaac ctccatcttt ctcctgataa ggtgggttac caattatcac atcaaatttc
    86701 acgtttttat aaactccttt caaattcgtt aaaccgtcat tgattgaacg tttcatactc
    86761 gtaactaatt cacttgagta ttcaacattt accttccatg cgttataacc aatcaaagtc
    86821 ctcttcgtta tcgtcttagc cattggagtt ttagaaatga caaaaatttg ctctgataat
    86881 actttttccc atattgcctc tgaatctacc cttccagcat taatatcgct ttgtttaagg
    86941 agctgaacat agtacatact cgttgctgca tataaaggat atagccctgt ttttgagttt
    87001 atctcaagaa tctttctatt ttccttaaaa atatcatctg tacagtcagt tttcctccat
    87061 cgcacgattg gttgaccgtt ctccgtagaa ttttggtatt cttcatcaaa gaaggaaagt
    87121 ccaccgatag tgttaccaag gtgcatatta acaactcgcc atggagtcaa tacggtttcc
    87181 ttgtctggat ttttaaaagt gctaaataat tcacctattt tcctagtacg ttcaataaca
    87241 tctaactcat cataagactt tgcacgtcta cggattatgc gtcctgcctc cacaaaaacg
    87301 tctgcatcat agtactcaat aaactctctg aatcgccctt tacttaaccc ctctggcatg
    87361 aactcattcc atgaagcctc atcaaccaaa ttaagaaaca tctcaatagt aacgtcatct
    87421 ttaatatcaa tgtccatccc ataaatcatc ataggtatgc gaatagaaac gcccctcaaa
    87481 atggaaatca ttttatttcg ctgctctcga agcctctttg ccctatctat tgcttcctgt
    87541 tcctctttgg tacgttgacg ttttggtttc ttttctgccc tcgaagcttt ttcatactcc
    87601 tcttcactca taccgttatc tgaaacaatg aagtctttca ctttggttgg tttacttgag
    87661 ccaacaattt ttttaaggtc ttcaaatttg gtaagatcag cttggtctaa atttaatagg
    87721 ttgtcattgt aaagagagct gtcttcaaag ccagtccgta cagctttttc tgcataagcc
    87781 ttcttcagtg ccgtaagcat tcggtcaacg ctaaattctt tcatcacatt cccctgttta
    87841 ccaaggatag gtaagaaatt tagcatgcgc tctagttttt tcttttgctc tgaactatta
    87901 acctttccct tcttgctagt tagtccgaca cttccactca taatttttaa agcacggtca
    87961 ggtgcaaaat caaagatgta gcagtttttc ttaattccaa gctctgcatg gttaaaaggt
    88021 gtctgtgcac ggaacgctgc ctgcagataa gatgtaggac tttctgtgtt tgaaaggaaa
    88081 aatacccctg tccactctgg aacattaact cctgttgtaa gcttgcgaac tgttaaggtt
    88141 atcgtcttag acttagctgg attaccaata gctttacgca ccaattctaa atctgaatca
    88201 ctagcatgct cactgtcacc tgtcttgact acgtttgcaa tattgtaatc tttgaatata
    88261 gggtgcgttt cgagcactct ttcaaaagca tttgcctctt taacccccgg catcagccat
    88321 aaagtatgcc gtaagttttc tctaaactct ggttttgaga aaggaaaatt agtcttttca
    88381 tcgtttgtag taatataatc aagaaacttg cgtacgtcac cttcatgaag caactttgtt
    88441 tcatcatctt ccttaaccct aaagaattct cggaagttaa atgccttatt ttcatcgaaa
    88501 taatcaaatt tatttggtat ttcaaaggtg aacatagata ctttcggtaa accttcgtat
    88561 gggttaggtt cattgggatg ctctactgac catttctcct tagcttcttg ctccatagta
    88621 taatcccaag tgaaaatatt atcctcatca tacttatcga aaaggttaaa tggtgtaccc
    88681 gaaagctcta agaccttggt atgctctttc cgcaacttag cctttacgtt attagctaat
    88741 tccgtttcgt ttccttcgtg agcttcatca ataataataa aatcccagtc aatcgctaag
    88801 aattcatagt ttttatcccc tgcttttcca cctgcccact cagaaccgcg taagtcctga
    88861 atacttgcaa aatagatgaa aggtttttcg gtatcaacaa ggttttgaat cttttctcca
    88921 tcttgttttg aaccatagat gtacgaacca tctgtgaaga tttttttgaa atccgtaaac
    88981 caaccatcac ttacaacagg tcgatgagtc atgataagaa cttttttgta ttggctctct
    89041 ttaatcactt gaagagaaga aagcgtctta ccaaaacgca tcttggcatt ccaaagcatc
    89101 tcatctttcg ttttgaatac agtcttagtc tgctcaatag catctttttg ttctggtcta
    89161 aaattaattt tgaactcgtc aaagtcatct ttttctgagt caagactacc tttaccatct
    89221 ttaaccgctt ggattgcttt cttggcaacg tcaactgtta cactgaacca ctcattaggc
    89281 ttccctgtgt catcttcgcc aatcgtttga ggattatagc ccgaacgctt taatacttca
    89341 tgcacgtcat ggtcacggaa aaattggttc ttcttattaa cagccaattg cgcccaatga
    89401 aggacaaaag gtacccccgc tgttgtcatg tactgcttta ttcttctact tgcatactct
    89461 cttaaatact cgctattagg agtaaagtct gcctcataat tctctggtac tgatgcatcc
    89521 ccaatctttt ccagtccttg gtacattttc catgtgccgt cagcatcctt ggcactctgg
    89581 atgtaaataa gtttacggtc aaaggtgtta taggtgataa caaacatctc atccgtaatt
    89641 tcttcctttg ggtttaacga tttatcccca gacagattgc gtgcaaagac tcttagcaag
    89701 gtatcaagag taactaaaga ttcgaccgcc tctgaaacag gtataattcc attttcatgt
    89761 gctgcaacat ttccatattt tctcaactta tgaaataagt cagctatcgt aggagtaata
    89821 ttatttctga ccctaagctc gcgaagtaca tcaacatact tcatactttg ctggtcaata
    89881 cccacatttg aagcaatctt ccgtgctgct acctccgaat aacgtctagt tccaatagca
    89941 acttcattag gtatttgatt aacggttaca cggtacgtgt taattgcatc tactaaatta
    90001 ggaatcccaa ggaagtgttc tagcttagaa ccatatttag gtaagtaatc gaagtattta
    90061 gctttagatt tcaacgctgt cgtcatcaat ttcctccatc tcttccttat aaacctcagt
    90121 tatcggcatc gctacataac gtaatattgg atcatttgaa acttcttcat cgacctcgtc
    90181 atcaaatgaa aacaaatcga gctgttcaaa tacaggcaga gctgagtatg cctctccaaa
    90241 ctctcgtgtt acccctgaat aaatatcctc caaagaatat tcagttcgat ttacagtgat
    90301 ttttttctgt ccttttctta gcgtcttagg tgtccactca ctaaaaataa tcggattatc
    90361 atctggtgtt cgttttgtaa gaaaatctcc ctgtttgata ttcctagaaa tcaaagtctt
    90421 tgcactgtct tggactttcc ttttcattgc ggtaccatgt tttttcgatt gttctctgta
    90481 caaatcgttg aatacttgaa acatgttcat tacacaggtc tgagcattgt cttcgagtaa
    90541 ctccacgcca tatagagttg aaagcgccag taaggagtaa ttttcatact gttctaggga
    90601 ctcattatat tcacgagcaa ccattgccat ttttctagtc aaaacctcaa ctaagaaagc
    90661 accttctcca gctgaaggct ctaagaaagt cgtctgcagc tcctcgcaag cctcttttat
    90721 gcctggcaaa tccaacatat ctttgacagt tctttgcggt gtaaatacct caccgtgctt
    90781 ctggacacgt tttttacttt tgattagtcg tccttgttcc tcttcaacca tcaccatatc
    90841 ttcaaccact aaaaatcacc catcttccca acacaaataa ccgaaacgga tgctacgaat
    90901 cggttatcac tttcatatat gggcttttgg gcagactgct ggctagtact gtgattgcgt
    90961 gtcatcatgc ccttttcctc cttacaagtc aagattttta cttgaaaaaa ctcgacaatt
    91021 tctaattatg cttatgttca attttaacac atttttgttt tttcctctat ctcaaaataa
    91081 agaacaaaat atagcttaac tggagaatta tagagtttgc ctcttgcaac acaggggagt
    91141 tagcgaagat gttcgtctgt cctattttat ttttatgaac atgaaaaaag accaccaaac
    91201 taggcagtct ttgacttatt attatttact tttagctatt cccattaaat tttaccactc
    91261 gtcccttttt cgccctcttt agatttagcc gagggtcgtg tggtaaaagc tcattattgt
    91321 cttaacgtgg tcaaaaacct ctttcctacg tttatttgat aacccatact gacgaaacac
    91381 ctctttcatt atcgcaatca tttgctcatt agataacttt tcatcatacc catcaatgaa
    91441 gttctctaat gactgactat ctaaagcttt ctttatttca aagaacaagt cataaacagg
    91501 tttatcttgc ttcttcagcc agtgaacata gccctccctg tgctcttttt cccgttcacc
    91561 tgttactcct tgctgtttag gtaatcacag aatgttgacc gactgacttc tatacctgta
    91621 tcttcttata ttctcgataa agacttctta atggtgagtc cttcttgata atacttcagc
    91681 ccagcctgta aaacttcgtc tggaaacctc tttcgatgtt ctgtcttgtt cgctgaaaaa
    91741 ctcttgtact catcatcaaa gctgagtgta aataccacaa caagatgttc gtacgatagt
    91801 ctgaccagaa aactgacttc cttgtcgttt gtacccaaat tttcatatga agtagataaa
    91861 agaactgtat acctggaaaa gacgtcttct tttacgaagt caatgcacaa tagacgtaga
    91921 ataggctatc ttcttcgaca gttagtgaac ctatatcgtc tgctaatagg atatccgact
    91981 ttaaatctaa atatttttcc tctgattttt taatttccct cttgaaataa tctattgtaa
    92041 tttgtttatc tgaattgttt gcaattaacc cctgcagaac ttctctagct tgttcaacat
    92101 cccgtattac catggaaatc actaggtatc aaactgagtc atacgaagac aaaaacataa
    92161 aaattgacct gttaaagggt cactgaataa catataaaat caataaaacg gagtcagaca
    92221 cggagtcaaa aaacgctgag agtaaaaaat atacagtccc ataggtaaag tgccacagga
    92281 ctgtatattt ttttatttca gattacctga aatgattcca tttacttttt atctcatttt
    92341 tccctactag ttagtatcaa atactctcct attttctcaa taaactattt tgggttacat
    92401 ggtatctgaa acagctaaaa ccttgtcatt acagcaaaaa atcactttat ctccgtttca
    92461 tttcagatac atttcactta agcggattcc caaaaaataa aagaaaaaga aacccctagt
    92521 tagcctatgc tttctaatga ttcctctcga aacacggtca aaaaacaacg gggtagggta
    92581 gttaataaaa agatagaaaa accattattc aatgactttc tcaaatattt cgttgtaatc
    92641 agttaccttt ccgttcttaa ataccacaga ataatctttt tttccatttt caagtaacct
    92701 aagtggaata tctttaccga cttgctctga caccgcatta ctgactcctg ttagattagc
    92761 taataaatct gataaagatt gagcatcagt cggaatctct gtatcagtat ttatcgttat
    92821 tcccttatct tcatttacaa gtttccaagt aggtggtata gccgcgtatg tttttatgaa
    92881 cgtatcaatt tttttatcta cagtttgtct tgatattgta tcgtttttct tttctacctt
    92941 accaattgga ctctcatctt tcgtagtcct ataaagagtg gctgttgata agtgttcatc
    93001 caaatttacc aacacagtta atttatcttt ttcaccgtca ataggcatga acgttatatt
    93061 gcttattccc gtggtttcct ttaattctgg aatcgcttta actaattcat cttttgatat
    93121 catgattgaa cgaaatgtag ttgaatcaaa ttttttatgt cttagagtat acttaaaatc
    93181 caccatttca ccattttttt gtagttctat tactctgtct ttagttattt taaaattata
    93241 gtctacagga attttctcag cagttcctga ccaatatcca tagaaatctt tatctttatt
    93301 tctacaacct gataattgaa taaccagtat agttcctata accaaaaata tacctgtttt
    93361 taaataattc tttttcatca taaagctcct cttttaaagc atttaatcaa caatgtaaga
    93421 acccaatttt taaagctctt taaaaacaat ttccatcaat tattataaca ttatttttct
    93481 catctatatt agtattttat ttacagggaa tcaaaaaaac tccttagtaa gcttacacct
    93541 ttaggagttc ctgtttggac ggtatacata atacatttaa atcaatttaa cattaacacc
    93601 tcagtatatg gtatctctac atatacacgg tctttctttg gatagtactc gacatttata
    93661 ttgatagcac ttccattgtc taacgtagct ttagcccggt atgtgttatc tccattttca
    93721 tgagtaatcg tcacatcatc acatgaccta tttaagttgt actgttcttg taaaatctgt
    93781 gtcaccaatg gtttagatga cttctctaat ccagatgtac ctgtcatttt tgaaaaaatg
    93841 atacctataa tagccaagac aacgatacct ataatgacaa acttcttgtt catatttact
    93901 ttattcaact ttttatctcc tatctccaaa ataatttgct cttatccctc ttatcagtac
    93961 attgacatcc ctaaaagtac accaaagatt gctccagcta cccctgctat catcaaaatc
    94021 gtacccattt tctcatctgt ttctcgatat aagtagccca taataacagc aacagcacca
    94081 aatacaacag gaataaatgc taacgatact accatagaaa cccaaccaat aatcaaatac
    94141 agcttccctg tctcttgtcc atgtacgatt tgttccttca tttcactaaa tgtctgctca
    94201 gtacttttct ttacttcgtc tgctccagtt gtctcatctg tttcttgtaa tgctccgcaa
    94261 aactggcaat acttactttc atctgttatt tctttaccac attttctaca atacatgtct
    94321 tctcctccta aaatcattca attgcttaat agttaactgt ttactttatt aaacacatca
    94381 gaaaacagct cgtttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt
    94441 ttaggaatcg ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt
    94501 ttggctaact ccgtttgaga aagtcctttt tcttttctgt atcggctgag attacttact
    94561 tttttcatga ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca
    94621 taaaatataa aaaacttcaa ataccattac aaatacaaca acttgttgta ctatactgag
    94681 atgggggtgt attaatgaaa cagaaacaat atgcgaaggt cattagagaa aagcgaaaag
    94741 ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt ctctacacaa caggcagtag
    94801 caagatggga aaattctgta acagagccta acctcgatag cttaacagct ctttctagag
    94861 ccctaggaac ccctgtaagc cattttctag acaatgttgt cgtagattat gaggaagagt
    94921 ttttggctct ctatcgttct cttagcactg aagatgcagt tcgaacaatt gactatatga
    94981 agctattaaa aaggcaagaa aatgaacgca atcagctatt gaaagattga aaaataaata
    95041 acccaccgaa ctttaacggt gggtgtattg agatgaaata agattaagag ctatggataa
    95101 cctgtctagt ttaatacaat tgggtctttt ctcttcctta ctctccgtcc atcatctctt
    95161 gttacacttg taaattgata cagaggaact atcccagctc ctgataatat caattctttt
    95221 attgtttcat ctatccctgc ccctttcgct aaatatatta aaaaactact acttgctttc
    95281 acattgtata gtttcttaat atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca
    95341 aagtagcctc tgttttttat taaagcattt ttcacgctgt tatccccaac attacgtagg
    95401 cgttcacaaa cagtgttaat aagttctttc tctgtcatta gatatgtccc ttgccacgat
    95461 aagccctgta aaacttcatg tgcttcatct aaaatcaatt gtttttcctt gctgagttct
    95521 gatcgggaat tttgaggaaa tactctttta gcctcttttt cgttaaaaca tatcttaacc
    95581 cattcataag aaaaatattg atatgtaaaa tgaatttttt ccagaagtgg aaggatatct
    95641 tcattcaatt tttgaatcac agaagttgca ttaaaaacgt ctaatcgata gacattgctt
    95701 ctcttatgat taaagtttcc taacgctttc tcttccttta acctatttag gtattcgagc
    95761 atcttcaaag gaacatcttc atcatctaat ttttttatca atcctataaa agtcataagt
    95821 gatagtaact tattacattt atctatgtca taagttttac catagtaaga actcattttt
    95881 ttagctaatt gttcccccga caaaatacaa gtcacacttt ccttactatc atcaaaaaaa
    95941 ttaacatcag aaagcaagac atcatagaca cgtcgcattt cctctagata attccttatt
    96001 gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa aaagtacttt tgattcctct
    96061 ttcaattttc gaaaatgtgt aggatacaag tctaggttct ctaatagcca attaaatgtt
    96121 tctacccgac tgtctttctt taaaattagc atcacaactc caataaaatt ttcactgata
    96181 tcacaattac tggaatgaca ataaaaaatg gaatgcccag ttttactggt aaaaatacta
    96241 gcagagggat tatgatcgtt gtgaaacaga catcttaagt ttttacttga cttaagtaat
    96301 ttattcatat caatggttaa caatctttca taaatctcat taaaagacat gtcggaggaa
    96361 tcaaacaggc agtctttaaa ccactgtttc atttcttctt tgtcgtcatt ttttatcaac
    96421 ttaacaaaat cggtttcatt gactagttca tactctctcc ctgaatcact ttgacaattt
    96481 ttagaacatg tcatggtaaa atcaatcgga agctcagtaa tatctaacat gttgtcttca
    96541 ttaattatta tcactgcatc tgacttatat cctccaaaaa acattctatt cgaagcattt
    96601 acattgtcat cacacccggg gatgttcttt ttcaagtatt cgtaaacagc aaccatttta
    96661 ccatagtcag taattgcttc ttttaataga aagacaatct tgtatttttc tagattttca
    96721 gagtaccgaa tacttttttg tatcgcaaac gcagaattat gaactaaatt gatagataaa
    96781 accttttcta cgcttaagta tctactatca tctttactca aatctactac cttccccttc
    96841 tcatctttaa cagtgttatc gacatccaga ataattactc tggtttccac aaaactctcc
    96901 ttgtcattgc ctttattctt tgtcttgctt ggaataaatg attgtgatga atttagtatc
    96961 tctacaaact catctcttgt aacttctcta tagttatcta aacacctatc tcttacatca
    97021 gttttttctt tttttgtaac tggcttctct aataattttt cttgatctac ataaattgga
    97081 atattaatca ttgatgtttc catttgtgtt tcctccttat aaaattctgt taaagactaa
    97141 aacatacaga taataaacac aaactcttta ttactatatg ttttagtctt atttgtttac
    97201 tcaaccttta tactgttcca tgaaactatc aatatcagat tttttgaatc taatgaccgt
    97261 atcaattttt ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga
    97321 tacacctaga tattcacttg cctgctgata gtccatatac tctagagtaa caccagaaat
    97381 aatgtcttct agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg
    97441 tatagaaaca atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattac
    97501 ttgaacaaat tttctaagaa tcatcttcgt attatttgat caaaataaaa gtattttgtt
    97561 gttattaacc gattaatcaa ataaaaaatg attatcagtt catcgacata cacataatac
    97621 ctcgatacaa aaggggtgtc aatatgttgt gtgaatttat ttacaacacc ccctttttga
    97681 ggtatactaa ttttaggagg tgtggtcaat atgataaagc gtccaccaat aaattattta
    97741 gaacgaaaaa agatactcgg aacaaaaata aaagctataa gaaagagtaa aaaattaaca
    97801 caaccagcat ttggtcttat gattaacaat ggtcaattga ttgacaaaaa aactatctac
    97861 gaatgggaaa agggaaccta tttaccaata ccagagcgat tatcccgaat tgctgatttg
    97921 ggaaatatga gtatagagga gttagtttgt ggcaatgtag aagaatatat tttgggaatt
    97981 attctttata gagatagcat tgtactagat ggtataacat ttccagataa aaacctgttc
    98041 cagcatcttc gtcaacagtt cccaccagta cactctaatt tagatacatg gctggataga
    98101 tattccaaat tagaaccaga gatgcaagaa tttattgcta acaagacttg taataaagtt
    98161 aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag aagaattatt catcaatgct
    98221 attatagaag aatttgacaa taacattctt ctgctcacta gcagtataga ggaacaacta
    98281 gaagaaatga tagatgaagg gatacatagt caagtggaag acatgaatta tcccgaagaa
    98341 gctgtaaacg aaattattga taatataaac aagctaaaac aaacgatttc atcaattggg
    98401 aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca tctagtggta agtactcctt
    98461 gtaaactaag aggagatgat ttcatggcaa aaatatcaaa gtacaaaaag aaaaacggtc
    98521 aagtggcgtg gatgtataaa ggacacattg ccaccgatcc acgaactggt gaaaaaataa
    98581 atactacgag aagaggtttt agtagcaaac aagaagctca aaaagattat gacgagtaca
    98641 gacatcaaat attatacggt gttaaaaaga aagcacctga tatgacattt gaagacctgt
    98701 ataatgaatg gataactcat cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag
    98761 taagatacgc aaataatcaa atacttccag catttggtaa gcttaaacta tcgaatatct
    98821 ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag taaatatgaa agttacgatt
    98881 atatgagaaa acagactgca caaattttac gctacggggt tgcaatgcaa tatattgaca
    98941 ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat
    99001 tttactcaaa agaggagctt aataatttac tggatgcttt taaagatttt ggtaacatga
    99061 aacaatacgc cttcttcaga ttattggcat acacaggtat gagaaagagt gaagtactcg
    99121 ctttacagtg gaaagacata gacacgttta ataaagaact acatgtcaat aaaactcttg
    99181 ctgtagatga atttggtaaa gttataattc aatctccgaa aacaagagct tctagacgag
    99241 ttatctcttt agatactgag actctttcta tcctcaacaa ctggaaatta cagcaaaaag
    99301 aagagtattt aaaacttgga tacaatacta gctctaaaga gcaacatgtt tttacaacag
    99361 ttaaaaacac tctatacatt ccaaacactg ttaatgattg gttaagatat attttgaaaa
    99421 aatataactt gcctcgtatt acacctcatg ggtttagaca tacacatgct agtttattat
    99481 tagaagcagg agaatctgtc aaggtagttc aacaacgatt agggcatgaa aatagtaaag
    99541 tgacattaga catctatgca catattacaa ataatgcacc taagaaaaca ggtcaagact
    99601 ttgctgatat gatggctcat cagtaaaaga gctggtcaaa agctggtcat ttctagtata
    99661 tagacaaaat aaaaaagcta gaacccttat ataccaagga ttctagctcc tataacaagt
    99721 ttatcctaca ctgccttcca tctcataact gatcaatcgg ttgagttcga ctgcatattc
    99781 cattggaagt tctttggtga atggttcaac aaagcccatg acgatcattt cagttgcttc
    99841 agattcggtc aggcctcggc tcatcagata gtagagttgt tcttctgaga tttttgagac
    99901 tttggcttcg tgttctaaag agacttggct gttgtggatc tcattgaatg ggatcgtatc
    99961 ggatttcgac cattcgtcca tgatgatcgt gtcacattcg atatgagaga tcgaacccgc
   100021 actgtttttg gcaaaggtta cttgtcctcg atagtttact tctccgccgt cttttgaaat
   100081 tgatttagag acgatagagc tagatgtatt tggtgcatta tggatcattt ttgcacccgt
   100141 atcttggatt tggtttgctc ctgcaaaggc gatggagagc atggtgcctc gtgcaccttt
   100201 tccatctaag taaacacttg ggtatttcat cgttgttttt gcacctaagt ttccgtcgat
   100261 ccattcaacg gttgcgcctt cgtatgcttt tgctcgtttg gttactaagt tgtacacatt
   100321 gtctgaccag ttttggatcg tggtgtaacg gcaatacgca tcttttcttg taaagatttc
   100381 tacgatggca gcatgcagac tattacttga ataagtaggt gccgtacaac cttcgacata
   100441 gtggatgctt gctccttcgt caacaatgat cagcgtacgt tcgaattgcc ccgtgttttc
   100501 cgcattgatc cggaagtacg tttgtaatgg aacatcgact ttgacgcctt ttggcacgta
   100561 aataaaggtg ccacctgacc atacggctga attcagcgct gctaatttgt tgtcggttgg
   100621 cggtacaagt tttgaaaagt actctttgaa taattcaggg tattctttca aagcagagtc
   100681 agtgtcagta aagacgattc ctaacttttc gaattcatct ttcatattat ggtacacgac
   100741 ttctgattca tattgtgcgg aagcaccggc tagataagct cgttcagctt ctggtatacc
   100801 aatcttttca aatgtatctt tgattttttc tggtacgtct tcccagtcac gagcgggttt
   100861 gtcacttggt ttttggtaat atttgatggc atcgaaatcg atgtctgaaa ggtcgggccc
   100921 ccatgtttgc attggcattt tattgaatgt ttctagagat ttcagacgga attcaagcat
   100981 ccattctggt tcgcctttga ttcttgacat ctcacggatc acttcttctg tcaagccttc
   101041 tcctgtactg aaaaccggtt cgacatcatc gtgaaacccg aatttatatt cttctaattc
   101101 tggtacgccc atgttctcac tcctcttcct tgtggtaatg atggaaagct gcggtaccat
   101161 cgttttccat tgcttttcct agtgctttcc aggcaagtgt cgcacatttg atccgagcgg
   101221 gaaatttagc tacaccacca agcatagcag catcgcctaa ttggtcggga tctttgactt
   101281 cttttccttg gacaagctgt agaaagtcta cagttaaatg ttctgcttct tctattgatt
   101341 ttccaatgac ggcatctgtc atcatcgagg cacttgctgt actgatggaa cagccgctgc
   101401 cgtcaaaggc gatatttttg atgattccat cttttacatc aacttgcaat tcgatcacat
   101461 ctccacatgt ggggttgttc aattcgatct tgcttgtcga gtcagtcaag cttccacgat
   101521 gatgtggatg ggaagaatga tccaagatca cttgacggta taaattgtct aatttagata
   101581 gtcccatgtt tgaaaaactc ctttgttgcc atgattgctt caactaaacg gtcggcgtct
   101641 tgcgttgtgt tgtagaaata aaagctggcg cgagcagtcg ctgatacttc aaggtacttc
   101701 aatagcggtt gcgcacaatg atgacctgct ctaacggcta ccccttccat gtctaaagct
   101761 gtcgcgacat catggggatg taacccatcc aaattgaacg caatgactcc tgtccgaaaa
   101821 ttcgggtctt gcggaccata aacagtcaat ccatcgattg cttgtaattt tggcaacaca
   101881 taagcggcta gttcttcttc atatcgatgg atattgtcca tgccgagttc ttgcaaataa
   101941 tcgattgcat gacctaatgc gatagcgcca gcgatatttg gtgtccctgc ttcgaatttc
   102001 caaggtagtt ctttccatgt actgtcgtat aattcgacaa aatcgatcat ttcgccacca
   102061 aattcaacag gttccatttc ttctaaccat tttcgtttac catataaaac accgatacct
   102121 gttggtccgc acattttgtg tccgctgaaa gcaaagaaat cacagtctag tgcttgaaca
   102181 tcaatggtca tgtgcggggc agattgagca ccatctacta cgatgacccc gccgttttca
   102241 tggacaagtt cagctaattc ttgaacagga ttgatgacac ctaagacatt ggatacatga
   102301 gcaatggaga cgattttggt tttgtccgta atctttttcg ctgcatcctc catatccagg
   102361 aagccttctt ctgttagttc gatatatttc aagacagcac ctgttcgttt tgccaactgc
   102421 tgccacggga taacattaga atgatgttcc atgtaagaga gcactatctc gtctcctgct
   102481 ttgatatagc ggtcgccgaa gcttctggct acccaattca agcttgtcgt cgtaccacga
   102541 gtaaacagca cttctgctgt ttcttttgca tggatgaacg cccgtacttt ttctcttgct
   102601 tgttcataat catgggtcgc gcgttctgct aacgtatgga caccgcgatg aacattggcg
   102661 ttatcgtgtt cgtaatagtg cgctaatgcc tggagtactt gttttggctt ttgggtcgtc
   102721 gctgcattat ccagataaac taaaggttca tcattgacgt cttgaaataa gataggaaaa
   102781 tcattgcgta gctttttagc atcgatcatc cattcaactt cccttcgatt acttcgacta
   102841 attctttccg aacttgttct acagggattg ctgttaagac agaaccgagg aatcctcgaa
   102901 tcactaagcg ctctgcttct tctttatgca atcctcgact catcagatag tacatttctt
   102961 ctggatcaac tcgtcctaca ctggcagcat gtccagcagt tacttcgttt tcatcgatca
   103021 aaagaatagg attcgcatca ccacgtgctt tatcagaaag catcaagaca cgactttctt
   103081 gttgggcatc tgcacctttt gcccctttca agatgtggcc gataccatta aaggtcaatg
   103141 ttcctttttc tcggatcact ccgtgttgca aaatatgccc gatcgtatga ggggctttat
   103201 tggttactcg cgtatcgatt ccttggattt gtctgcccga gctgattgcc acgattttga
   103261 cttctgcgtg tgcaccttct cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt
   103321 cattcatgac accaattgcc cagtcgacag aagcatctcg taaaatgtgc ccacggcgat
   103381 tcatataaga agtaatattt tctcctaatt gatctacggc tgaatatttg atttttgaac
   103441 cagctttagc gatgacttcc acgatgatat tacctgatga ttttgctact tgttctttgg
   103501 ttgtctggaa acgttccaga taagaaaatt cactatgttc atctgccacg atcaacacat
   103561 gcttgaagaa gtgctcatcg gatgcgccat cttgaatgaa taaggactcg agtggttcat
   103621 cgatcacgac atttttaggc acatacagga aaataccgct gttcataaaa gctacatgga
   103681 gtgcagtcaa ttggtcttcg ttcatgttta ctgctttttt catataatat tcttggacta
   103741 gttctggata ttcgatcatt gcagtgaaca agtcggtaaa aatgactcct ttttctgcta
   103801 attccacagg cagttgttca aaaatcgtcc atgaaccttg ttggacaagc acaggattgt
   103861 ctttcattgc atcaaatgcc ggaatcgtgc ctgtttcact gatggtttgt gtttctttca
   103921 catcaaataa cgaccagcga tggaatttca cccgatcgat atgcggcaat ggcagttcat
   103981 ctgctttcgc tagtgcgtct tgacggaagg ttctcatcca atcaggttcg cctttactta
   104041 aagaaaaatc atttaccgcg tcaagataat cgagccagtt tttttctttc attcttgctc
   104101 ccccttacgc ttcttcttct ttatagtcga ttcctaattc tttactgata ccagcgtagc
   104161 cttctgcttc cagacgttta gctaggtcag cattccctgt catgactact cggccttcca
   104221 tcatgatgtg aacgacatca ggtgtgatat agttcaataa tcgttgatag tgggtgatga
   104281 tcaatgcacc gaaattgtct ccgcgcattt catttactcc tttagcaacg actttcaaag
   104341 catcgatatc cagacctgaa tcgatttcgt ctaaaattgc aaatgttggt tctaacatca
   104401 acaattgcag gatctcattc cgtttctttt ctccgccaga aaaaccttcg ttcagataac
   104461 gttcagccat ttcttctggc atatttagta aagccatttt ttcatctaac tttttcaaaa
   104521 atgccataac agaaattttg tcgttttcat cacgtttcgc attgatcgct gcacgcatga
   104581 attccgcatt cgtgatacct ggtatctcac ttggatattg catagctaaa aataagccaa
   104641 gtcgcgcacg ttcatctact tccaagtcta aaacattttt cccgtcaaac aaaatttcgc
   104701 cttctgttac ttcatagttt ggattgccca tgatagcggc agataaggta gattttcctg
   104761 tgccgttagg tcccatgatg gcatggattt ctccggtttt cattgttagg tttacgcctt
   104821 taaggatttc tttttcctcg atcgacacat gtagattttt tatttctaag acagacatgc
   104881 attatacccc caaattctct ttcgttctca ctcattttag actaattgag aatgaagcgc
   104941 aatgaccttc cttactaaaa tcaaatatct tttatttatt attaaacaat tttgatttct
   105001 aaattagaat aggtataaaa aagatgagaa ataagccatt acagacttat ttctcatctt
   105061 ttttgttcca taccttattt tgcatcagcc tttactggtt taacagagcc gccccatttg
   105121 tctgtgatcc attcttggac ttctttgcgg tgaagcactt caatcagttt tttgattttg
   105181 tcactgtctt catcaccttt acgagtcgca ataatattca cgtaaggaga gttgtccttt
   105241 tcaagcaata cggcatcttc taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg
   105301 attgcaacta ggtcaccttc atcattttgg tacgtagaag ctaaaagtga tgggtcgatc
   105361 gtatggttga acttcaagtt cttagggttt tcttcaatat catcgaatgt tgctgtttcc
   105421 agatctactc catctttgac tttgactaag ccagcatctt gtagaattgt gatgatgcgt
   105481 ccccagtctg attcagaatt tgaagtaatg attgttgcac catcttttaa ttcggaaaca
   105541 tctttgactt ttttagagta caagcccatt ggttcgatgt ggacagcacc ggcattgaca
   105601 aagtcatatc ctttttcttt gatttctttg ttcaaataag gaatatgctg gaagtaattg
   105661 gcatcaatgt cgccttcttc caaagattgg ttcggtaata catagtcatc gaatttgacg
   105721 atttcaagat cgattccttc atctttcaat aatggtttca catgttccaa aatctctgcg
   105781 tgcggtgtcg gtgaagcgcc gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct
   105841 ttgctgtcgc tgttgttacc gcatgctgct aatcctacag tcagcaaaag cgctgctgca
   105901 aatccaaaaa ttttcttttt catatcctac atccccctat atcctattat cttttgtctg
   105961 ttttcttagt taagaaatca ccgattcctt gaacgataaa tacgattagt aaaataatta
   106021 aagtcgcaac gaatgtcact gtcagattac cacgctggta gccttcctgc caagcaagac
   106081 ctccaagtcc acctgcgccg atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg
   106141 ttgtaactgt taatccagaa atcaatgcgg gaatactttc aggtatcaag accttccaga
   106201 tgatttccca gtaacttgct cccattgcat ctgctgcttc cagtacacca gggctgactt
   106261 cacgaaaagc aatctctact agacgggcat aaaatggtgc tgcggataaa acaagagctg
   106321 gaatggctgc ttgcgcacct agcatagtgc ctacgattgc tttagtaaat ggaatgatca
   106381 aaaccatcag gatcataaat ggcgttgacc ggaaaatatt actgatgatc gatacgattc
   106441 cataaatgat tcgagcatac ggtttgtttt tgcgtccaat agagtaaagg atcaatccca
   106501 aaactaatcc gatgatcgtc actaagacca ttgagattaa tgtcatatat agtgtatcaa
   106561 ggatagcctg attcatcgtt tccgggttga tttgtttaaa atctagatat gtttctgaaa
   106621 agcttttatc cacgatggat cacctcaatt cctactcctt gttctttgat gaacacttct
   106681 gcttgatcta attctttggt ttcaccagtc atcaagacag tcaatgaacc aaaggaacct
   106741 tctttcactt gtttgatctt tccatacaca acgttgacat cgatgttata gcgtcgaatc
   106801 gcttgggaaa tgactggctc gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg
   106861 ccgcttggat tttctttgat gagttcagct aatagttctg tcgaatcagc ttctggttcg
   106921 atgtcttgtt gtacaaagcg tttggttact tcttttttcg gttgtctgaa gacagtcaat
   106981 acgtctcctt cttctacgac tttccccaac tccatcacag cgactttgtt gcagattttg
   107041 cggatcacat tcatttcatg tgtgatcaag acgatggtca aattcaattt cttattgatc
   107101 gctaacaata agtctaatac ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca
   107161 tcacaaagca ggatctcagg atcatttgcc aatgctcgtg cgataccgac gcgttgtttc
   107221 tgtccgccgg aaagttgtga aggatatgct tgtccgcgtc cttctaaccc gactaaacgc
   107281 aacagttctt ctgcccgttc tttacgtttg ctctttggta cgccagctaa ttccagcggc
   107341 agctggatat tttcaagtac agttcttgac cataacaggt tgaaatgctg gaagatcata
   107401 ccgattttct ttcggaatgt acgtaattct ttgtttttta attttgtaat atcttgatcg
   107461 tttacgatca cttcgccgct cgtgggaagc tcaagtccat tcaggagacg gactaatgta
   107521 ctttttcctg cgccagaata gccgacgatt ccataaatat cccctttttc aacgtttagt
   107581 gaaacgtcat tgacggcttg gatgtttccg tttttcccat tgaatgtctt tttgacattc
   107641 gttagttgga ttaatgacat gccattcaca tctttctctt atgatttttc aaacaaaaaa
   107701 gccttcgccc tcttgcctta agcaaaagga cgaaagcttg aacttccgtg ttaccacctt
   107761 gatttaccgt tacttcgcaa taacagcctc ttccagtact tggacttagc ttctttccgt
   107821 atactgtcgc gctgtaacgg gcactcccgt agtaagtttg cttacgctcg cttactctgc
   107881 tctaagacca tcttccatct cattttcgtt acccattttc agcaaacagg gctctcttca
   107941 aacgtccgtg acatgtactc ttcttttcaa agctttctcg tttcatatag gattgcttat
   108001 gattatagca actggaagaa tcagtgtcaa cttttttcaa gtgattttct gattttataa
   108061 ttcgtcaaat tcttttggat caacatcttt gaaacttaag atccacgcat tcatttcatc
   108121 tgaatcattc aagatattga tatcttcacc gattttatca ttaacagaag aaactgtacc
   108181 cgttagcggg ctggcaaatt cattgaccgc tttttccgct tcaacttcga tcagaggttc
   108241 atcttttttt agcgattgtc ctgcttttgg cagattggca aacgtgatgt ctccaagttc
   108301 ttcttgcgct tcatttgtca aaccaacaca gtattctttt ccattaaata agatccaaag
   108361 gttgtccttt ttcttcaaat gcttgcctgc cattttatat cagttctcct tcataaattt
   108421 tttcattgaa tccaattgca acaagctggt cctctcgtaa gatcagtggc cgcttgatca
   108481 gcataccatc tgtcgataag acttggctcg cttttgaagc cgtaaactcg cctacttgat
   108541 ctttcagtcc aagttcccga tatttcatcc cgcttgtgtt gaacaggcga cggattggta
   108601 cttgactcgt ttctagccaa gtctcaatcg ttgaggcctt cggtacttct ttgatcatat
   108661 caacagtttg ataactaacg ccatgctggt ctaaccacgc tttggctttt ttgcatgttg
   108721 aacatttcgg gtaccagtaa aaggtgtaca attttcttac ctccttgaag atcttgcagg
   108781 tgcttcattt tggtatctca tcgataatat caacccgata ccgatcatgt tccctaaaat
   108841 cgaagatcct ccctgactaa tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat
   108901 atttgctcca atattctcaa agacatggaa cagcagcatc atgatcaaac cgcttgcgat
   108961 ataggcgtaa aattcattat tcgtatcgaa acacacccga atcatacgat agattaaaat
   109021 aaaatacaag aaaatgacga atgcaccacc gataaagccg aagttttcac cgataacggt
   109081 aaaaatcata tcggactcac gaacaggcac ataaatattg cttacgttga atcctttacc
   109141 aaacaagcca ccggtaccaa ttgccaaaag tccttgggcc ggctgataac tcattcctga
   109201 cgtatcatgg aaagggtcca gccatgaatc aatacgtgca aactgataag atttgaagcc
   109261 aactttgtaa agcaagtctc ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc
   109321 aacgactact gccaagatta ctacaggtgc gatgatgcgc caagagattc cagacatcaa
   109381 gaaaatcccg ccaaagatcg caagaaagac aagcatcgtt ccaaagtcat cttgggccat
   109441 gatcaatgct aaaaccggta ttgtcacgat caagagcttc ccaatcagcc aaaagtccga
   109501 tttcatcgtc cgttctttta cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa
   109561 gatgtacgca atcttcatca actctgctgg ctggaaagta aacgcaccga atcgaaacca
   109621 gtttttagac cctgttgaag cagctaaatt ctgatcatag aatttgagca agagtcccat
   109681 taccactaac cccaaaccat aaaggtaagg agtcagtttc cagatccatt tggaattcaa
   109741 atgcatgatg acaacgattg ctgccgctcc aactaaatac cagacaactt gcatccctac
   109801 gcctttcaag actgctggat tgttcggatc atggctaagt gctacatata atgagagcag
   109861 accaatcaaa cataacagaa agactggtaa aatcacgcca taatcaatac gattatcgtt
   109921 cgaagctttt aatttttttt ccatgtttca tcccttttct taaaaaccat actgctgatt
   109981 attatagcgg tccagttaga taatggaaag tctttccata gacatttggg aaaaattaac
   110041 tttccgtttg tgcagttgct tgagcaaatg cagtacaaaa gcctactgct tatctttagt
   110101 gtatcataga actttttttg atacatgcaa ttatctacca gctgagattc tcctcgctgt
   110161 gagaagtttc tgtgctttca ttgtatcgaa ggatctcttc ggcaatctct agcagctgat
   110221 cagaaaccag attggttcgt tggatgaaat aaaaatatcg atggaaattc aacggagtca
   110281 ttggcacatt ttctggaaca gagcgcttag acaaaatcga gcgaccgatt ccttccttca
   110341 gcaacgcaac aattacatcg ttacttttca cgatcatctt cttatcttgg atattcatct
   110401 cttcaaaata acgctgcgta taatgaaaaa caccagagtt tgcttctctt acaagccaca
   110461 atttgctttt tgggtcccca gcgatgacta gctggtcttc catcaaagag tatctttgta
   110521 cgccggaagt ctctaatggc ttttcgataa aaccaaaatc ggattcatgt ttcgctactt
   110581 gttctaatga ttcatgagaa ttcgcgattt cgatctcaaa atgcacttta ggaaaccttt
   110641 tgatcagatg tttcatcaac tgaggcaaaa gatagacagc aaaggtgtgg gaagcaacga
   110701 tccgacatac ttcttcttca ttttgttcat tgagcaattt ttgctgagtt tcttcccatt
   110761 cttctaacaa aaccagtgtc cgttcatata ataagtcggc ttgcttagtt ggaagcatct
   110821 cttttcttcc gttacgttga aaaagatcga tattcaattc ccgctccaac tgtttgattt
   110881 gattcgagac tgctggttga gataaaaaca gtacttctgc tgctctggaa aaatttcttg
   110941 tttcatatac tgctcgaaaa gtcgttaata acttaaacat ggcaccactt ccataacttt
   111001 ttatgataat tactataaaa actatttgat ttaaatatac aaaaaaccat ctataataga
   111061 aatgtacaac aaaacagttg ctgatccaac tagtaactta gatgaagaca atcagctgtc
   111121 tgcttgctcg taatcataca ttcctattca ttttacaatg tattcgttca tcttggtact
   111181 aaaatctatt aaataaaaag gagaatatta tatatgaaga actttttgaa aattattccg
   111241 atcccgatct gcgggttgat ccttgggtta gcttcccttg gaaatctgtt taaagactat
   111301 caccatgttg ctttaggcaa tctaactggc ggtatctcta tgattcttat gatattcatt
   111361 tttgttaaat tgtttatctt attcgaacac acgaaacaaa cgttgcatga tccaatcatc
   111421 gcctccgttt caccaacttt tacgatgtca ctcatggtta tttgtactta ttttgtttca
   111481 tatgaaagta ttgcaccaat tgtcaaattc atttggctgt gcgctgttat cttccaagtg
   111541 atccttgtct tttatttcaa ttatcaccat gttatcaaag ctgatctttc aattgaagca
   111601 atttatccaa gctggtttat catttacgtt ggatttgggg tcatcactgt aactgccggg
   111661 aacttcttcc cgcttattgg aaaaattttc ttttggattt cattgatctg ctatgcgttc
   111721 ttattgccga tcatcatcaa acgtgtcttt ttcgtaagaa atatggctca tccgacacgt
   111781 ccgctgatta cgatcgttgc agcacctggt tctctttgcc taacaggtta tcttaaaaac
   111841 tttgctgaac ctaattttta tcttgtcgta gcattgttcg ctttatctca agtattgtat
   111901 ttcgttgtgc tgtctatgct tccacgtttg ttcaagcttc gtttttatcc aagctatgca
   111961 gcctttacat tcccgcttgt tatctctgca acagctatat ttactactgt tcattacttc
   112021 aacagtttag gtatctatag tagcgtattg gatttcttaa aagttgcgga atgtctgttt
   112081 gcaagtgtag ttgtcttcta cgttcttgct cattacgtac gtttcttaat taaagaacat
   112141 agcaaacatg gaaaatttgt ttctaaaaaa atcgcttaat aatcgattca ataggttgtg
   112201 aaaaagctgt tctgcatcaa gtaataagaa cggccaaaca aaaacagctg ttcatgtttt
   112261 tgtactggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtc
   112321 gtaaaagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat
   112381 ttttgtgaaa tttcggctta tttccgaaga agctacttct ggaacaccgt ttaccaagag
   112441 gtcgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
   112501 catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc gtttattcga
   112561 aacaagaggt cgtgacaaga cttttgtcac gacctcttgt tcttttgtca aaatactggt
   112621 ttagtgctac tatgttcgag tatgctttta gtatttattg acgaaaggaa ccgatcaaat
   112681 gaaatcattg tcacatccaa taaaatctgt aaaggattca gcggtccgcg aaatcttacc
   112741 cggtttactg ctttcttttg ccatcgctgc cttcagcagg cttcttgcta tttatctgcc
   112801 acagttaggt ggagcgacta ttgctatttt attgggtatt ttattaggaa atactttttt
   112861 taaacagcct gttctagcaa tcggcacaaa atttgctgaa agcaaactat tagaattctc
   112921 tgttttttta ctaggtgcaa ctgtcacagc tcaaacgatt gctcaaattg gtataaaagg
   112981 aattgttttt gtgttactgc aaatgtcagt cactatcatc ggtgcttact tgattggtaa
   113041 aaaactgcaa ttcagcgata cttcttcttt gatgatggct ggaggaaacg ctgtttgtgg
   113101 ttcttctgct attgcttcga tcgccccttc catccatgcg aaagaggaag aaaaaggaca
   113161 gatcatcaca ctagtcaatc tactgggtac tgtgatgatg ttgactctcc cttttcttgg
   113221 attacagttg tttggtggaa atctcattga aaaaagtgca cttttgggtg gcacactcca
   113281 atctgttggt caagtagtcg ctggggcaag cttacttgac aattctgttg tcgaattcgc
   113341 catgctattt aagattctac gtattatgat gctcgttgtt gttgtctatc tgtttgaaaa
   113401 attcatgcac aagaaacaac cagctctgtt tgaaacgcca actgatacgc agaaaaaacg
   113461 aaaattacca cttccttggt atgtattagg gtttgtgatc ttttgtttgg tcaacagctt
   113521 gttttcttta cctgtagtta ttgaccaatc ggcacatttt atcagcagct ggtttgagat
   113581 cactgcttta gcggcaattg gtctgcgttt agactttgca aaattcatga aagaaggtac
   113641 acgcttctta ttttatggat tagctgtcgg gcttctccaa acagtgatgg ctttactatt
   113701 tatttggctg atcaaagttt agaatgaaca tgaaaaaaca gttgtgattc tcgcatggaa
   113761 caatgtccga atcacaactg tttttaattt tgcagatatc cttctaacaa caacaattgg
   113821 cgtttgattt caaccccatc tattgcgttt cccatatatc ctgtcagttt tccattttgc
   113881 ccgatcactc gatgacaagg aataacgatt ggcaatggat tggttcgatt tgcctgccca
   113941 atcgctcgca cagctttcgg actatcaaca gcaacggcta cttctttata cgttcggatt
   114001 tctccataag gaatggtcga caaagctttc cagacatttt gctgaaaagg cgtacctctc
   114061 tttatagata aaggaacatc aaattctttt ctttttcccg caaaatattc ttcaaactgt
   114121 tcttgtgtgc gcgttaaaat ttgctgattc gctgattcgc tttctgaaat tggctgaaaa
   114181 gatacagcag tcaatccgga ttcatctgca tctaaccata aaaagccgat aggtgtgtgt
   114241 attttcattt tgtttgcttc tagtcaatag actggaactt cctccttttt ctctatccaa
   114301 aactacttgc caactaataa aaattgttct taaaagtttg ataaaccgag gaaacatggt
   114361 aaactaaata cgttagttta caaaaagaat acctgttctt ttacgtaaaa tcaaatttta
   114421 aaggtgttat ttaaggttta ttaaattttg aatgtaaacc atatataaac atacgattga
   114481 aaggatagaa acgatgaaag tatcaattgt tgtcccatgt tttaatgaag aaaaaactgt
   114541 tcctcttttt tttgatgagg ttgaaaaatt caaaggtgcc catgagttcg aatatttgtt
   114601 tattgacgac ggatcaaaag acggtacgtt agcagcaatc aagaagctag cctcagctta
   114661 tccttatgtc cgctttgtct cattttccag aaattttggg aaagaagcgg ctctatacgc
   114721 tggattgcaa gcttctacag gagatttagt gaccgtgatg gatgttgatc tgcaagatcc
   114781 gcccgaattg cttcctgaga tgatcaaccg gatcactact tctgatatcg attgtgtcgg
   114841 cacccggcgt tcgactcgtg aaggcgagcc tgcgatacgc agtttctttg cgaaaaaatt
   114901 ctaccagttg atcaataaaa tcagcgatac agaaatggtc gatggcgctc gtgatttccg
   114961 cttgatgact cgtcaaatgg tggatgccgt cctagaatta acagaataca accgattttc
   115021 aaaaggatta tttagctggg tcgggtttaa aacagaatat atttcttatg aaaaccgcga
   115081 acgtgtggct ggcgaaacat cttggtcctt ctggaaatta ttcaactact cgattgatgg
   115141 tatcgtgaac ttctcggatg caccgttaac gattgcttca tttatcggcg ctttgtcttg
   115201 tgtcggttct ggtattgcaa ttttgttcat tattttgcgg gcattgttgt ttggtgatcc
   115261 tacatcagga tggccttcca tggtctcgat cttcctgttc attggtggaa tccaattgtt
   115321 atgtcttgga atcatcggga aatatatcgg gaaaatcttc ttagaaacga aacagcgtcc
   115381 tgtttatatc ataagagaaa cagaaaaagt gaagccaaat gaattgaagc ctcaccaata
   115441 atatacgtca aatgaagtaa acattttttt aaatgaaaaa ggactgtgac aaaagtcttg
   115501 tcacagtcct tttttccgaa taaatggtgt tccagaagta gcttcttcgg aaataagccg
   115561 aaatttcaca aaaatttgag aagcaa
//
LOCUS       DANJMA010000003        93455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000003 DANJMA010000000
VERSION     DANJMA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            85..972
                     /locus_tag="PWU31_000238"
     CDS             85..972
                     /locus_tag="PWU31_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL1082816.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="PWU31_000239"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="PWU31_000239"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL1082817.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="PWU31_000240"
     CDS             2274..2720
                     /locus_tag="PWU31_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1082818.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="PWU31_000241"
     CDS             2993..4345
                     /locus_tag="PWU31_000241"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL1082819.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="PWU31_000242"
     CDS             4516..4803
                     /locus_tag="PWU31_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082820.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="PWU31_000243"
     CDS             5029..5472
                     /locus_tag="PWU31_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1082821.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="PWU31_000244"
     CDS             complement(5478..6662)
                     /locus_tag="PWU31_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL1082822.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="PWU31_000245"
     CDS             6930..7628
                     /locus_tag="PWU31_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL1082823.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="PWU31_000246"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="PWU31_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL1082824.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="PWU31_000247"
     CDS             complement(8438..8971)
                     /locus_tag="PWU31_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082825.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYSPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="PWU31_000248"
     CDS             complement(9268..11649)
                     /locus_tag="PWU31_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL1082826.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="PWU31_000249"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="PWU31_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL1082827.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="PWU31_000250"
     CDS             12350..12889
                     /locus_tag="PWU31_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL1082828.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="PWU31_000251"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="PWU31_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL1082829.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="PWU31_000252"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWU31_000252"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="PWU31_000253"
     CDS             13996..15162
                     /locus_tag="PWU31_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL1082830.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="PWU31_000254"
     CDS             15208..16704
                     /locus_tag="PWU31_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL1082831.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="PWU31_000255"
     CDS             16785..17468
                     /locus_tag="PWU31_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1082832.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="PWU31_000256"
     CDS             18113..19303
                     /locus_tag="PWU31_000256"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL1082833.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="PWU31_000257"
     CDS             19405..20358
                     /locus_tag="PWU31_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL1082834.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="PWU31_000258"
     CDS             20427..21776
                     /locus_tag="PWU31_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1082835.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMIFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="PWU31_000259"
     CDS             complement(21959..22282)
                     /locus_tag="PWU31_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082836.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="PWU31_000260"
     CDS             22411..23574
                     /locus_tag="PWU31_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1082837.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="PWU31_000261"
     CDS             23644..24993
                     /locus_tag="PWU31_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1082838.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="PWU31_000262"
     CDS             complement(25038..25469)
                     /locus_tag="PWU31_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1082839.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="PWU31_000263"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="PWU31_000263"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL1082840.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="PWU31_000264"
     CDS             28699..29295
                     /locus_tag="PWU31_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1082841.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="PWU31_000265"
     CDS             29417..30565
                     /locus_tag="PWU31_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1082842.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="PWU31_000266"
     CDS             complement(30603..31016)
                     /locus_tag="PWU31_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL1082843.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="PWU31_000267"
     CDS             31095..31526
                     /locus_tag="PWU31_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL1082844.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="PWU31_000268"
     CDS             complement(31625..31858)
                     /locus_tag="PWU31_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL1082845.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="PWU31_000269"
     CDS             32041..33711
                     /locus_tag="PWU31_000269"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL1082846.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="PWU31_000270"
     CDS             33858..34244
                     /locus_tag="PWU31_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082847.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="PWU31_000271"
     CDS             34231..34929
                     /locus_tag="PWU31_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1082848.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="PWU31_000272"
     CDS             34934..35398
                     /locus_tag="PWU31_000272"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL1082849.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="PWU31_000273"
     CDS             35395..36306
                     /locus_tag="PWU31_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1082850.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="PWU31_000274"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="PWU31_000274"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL1082851.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="PWU31_000275"
     CDS             37094..38377
                     /locus_tag="PWU31_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL1082852.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="PWU31_000276"
     CDS             38390..39316
                     /locus_tag="PWU31_000276"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL1082853.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="PWU31_000277"
     CDS             39335..40615
                     /locus_tag="PWU31_000277"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL1082854.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="PWU31_000278"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="PWU31_000278"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL1082855.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="PWU31_000279"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="PWU31_000279"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL1082856.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="PWU31_000280"
     CDS             44940..45716
                     /locus_tag="PWU31_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL1082857.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="PWU31_000281"
     CDS             45713..46645
                     /locus_tag="PWU31_000281"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL1082858.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="PWU31_000282"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="PWU31_000282"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL1082859.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="PWU31_000283"
     CDS             47365..47994
                     /locus_tag="PWU31_000283"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL1082860.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="PWU31_000284"
     CDS             47982..48296
                     /locus_tag="PWU31_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082861.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="PWU31_000285"
     CDS             48334..48975
                     /locus_tag="PWU31_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1082862.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49018..49710
                     /locus_tag="PWU31_000286"
     CDS             49018..49710
                     /locus_tag="PWU31_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL1082863.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="PWU31_000287"
     CDS             49707..50609
                     /locus_tag="PWU31_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1082864.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="PWU31_000288"
     CDS             complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="PWU31_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL1082865.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="PWU31_000289"
     CDS             52663..53286
                     /locus_tag="PWU31_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082866.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="PWU31_000290"
     CDS             53736..54785
                     /locus_tag="PWU31_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1082867.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="PWU31_000291"
     CDS             54782..55663
                     /locus_tag="PWU31_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082868.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="PWU31_000292"
     CDS             55666..56436
                     /locus_tag="PWU31_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082869.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="PWU31_000293"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="PWU31_000293"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL1082870.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="PWU31_000294"
     CDS             57747..58910
                     /locus_tag="PWU31_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1082871.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="PWU31_000295"
     CDS             59027..59437
                     /locus_tag="PWU31_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1082872.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="PWU31_000296"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="PWU31_000296"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1082873.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62010..62294
                     /locus_tag="PWU31_000297"
     CDS             62010..62294
                     /locus_tag="PWU31_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082874.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62454..62690
                     /locus_tag="PWU31_000298"
     CDS             62454..62690
                     /locus_tag="PWU31_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1082875.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63086
                     /locus_tag="PWU31_000299"
                     /pseudo
     CDS             62687..63086
                     /locus_tag="PWU31_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(63753..64697)
                     /locus_tag="PWU31_000300"
     CDS             complement(63753..64697)
                     /locus_tag="PWU31_000300"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL1082876.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64993..65937)
                     /locus_tag="PWU31_000301"
     CDS             complement(64993..65937)
                     /locus_tag="PWU31_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082877.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66158..67372
                     /locus_tag="PWU31_000302"
     CDS             66158..67372
                     /locus_tag="PWU31_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1082878.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67394..69409
                     /locus_tag="PWU31_000303"
     CDS             67394..69409
                     /locus_tag="PWU31_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL1082879.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69492..70865)
                     /locus_tag="PWU31_000304"
     CDS             complement(69492..70865)
                     /locus_tag="PWU31_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1082880.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70891..71175)
                     /locus_tag="PWU31_000305"
     CDS             complement(70891..71175)
                     /locus_tag="PWU31_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1082881.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71177..73192)
                     /locus_tag="PWU31_000306"
     CDS             complement(71177..73192)
                     /locus_tag="PWU31_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1082882.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73371..73676)
                     /locus_tag="PWU31_000307"
     CDS             complement(73371..73676)
                     /locus_tag="PWU31_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL1082883.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73765..74796)
                     /locus_tag="PWU31_000308"
     CDS             complement(73765..74796)
                     /locus_tag="PWU31_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1082884.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75003..75878
                     /locus_tag="PWU31_000309"
     CDS             75003..75878
                     /locus_tag="PWU31_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1082885.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76030..76872)
                     /locus_tag="PWU31_000310"
     CDS             complement(76030..76872)
                     /locus_tag="PWU31_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1082886.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76964..78841)
                     /locus_tag="PWU31_000311"
     CDS             complement(76964..78841)
                     /locus_tag="PWU31_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1082887.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78838..80259)
                     /locus_tag="PWU31_000312"
     CDS             complement(78838..80259)
                     /locus_tag="PWU31_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1082888.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80262..83651)
                     /locus_tag="PWU31_000313"
     CDS             complement(80262..83651)
                     /locus_tag="PWU31_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL1082889.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83908..85299
                     /locus_tag="PWU31_000314"
     CDS             83908..85299
                     /locus_tag="PWU31_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082890.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85372..86019)
                     /locus_tag="PWU31_000315"
     CDS             complement(85372..86019)
                     /locus_tag="PWU31_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1082891.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86027..86713)
                     /locus_tag="PWU31_000316"
     CDS             complement(86027..86713)
                     /locus_tag="PWU31_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082892.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86719..87792)
                     /locus_tag="PWU31_000317"
     CDS             complement(86719..87792)
                     /locus_tag="PWU31_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082893.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88014..88688
                     /locus_tag="PWU31_000318"
     CDS             88014..88688
                     /locus_tag="PWU31_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1082894.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88693..90033
                     /locus_tag="PWU31_000319"
     CDS             88693..90033
                     /locus_tag="PWU31_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1082895.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90182..91168)
                     /locus_tag="PWU31_000320"
     CDS             complement(90182..91168)
                     /locus_tag="PWU31_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL1082896.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91892..92923
                     /locus_tag="PWU31_000321"
     CDS             91892..92923
                     /locus_tag="PWU31_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1082897.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93032..>93455
                     /locus_tag="PWU31_000322"
     CDS             93032..>93455
                     /locus_tag="PWU31_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL1082898.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29896 a        16575 c        18965 g        28019 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggagaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtggaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atgatctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaagaac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta ccgtcactcg ccaggcgaaa
    62761 tgatcggtgt aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag
    62821 tattcaatca agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt
    62881 tagctaaaaa acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt
    62941 tgtttcttca attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta
    63001 tcataatgat taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt
    63061 atttacgaca aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag
    63121 catgtcatta aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat
    63181 aaaaatatgg aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag
    63241 tgaatagtag gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta
    63301 cataatatat attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt
    63361 tatcaaaaat cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg
    63421 cagtttttct tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag
    63481 ttaatcaaat atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt
    63541 ttaattaacc acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt
    63601 tcatatgttc atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat
    63661 ttgcttacct attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag
    63721 ttttaggatt tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt
    63781 aaagcacttg ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca
    63841 acttcacgaa caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg
    63901 tcgtattcac cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata
    63961 gcttttacga tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca
    64021 atgatctcat aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca
    64081 atacgatggt ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac
    64141 catgcagcaa cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg
    64201 tcaactttta atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca
    64261 gtaccgataa cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata
    64321 tctactgggt ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt
    64381 acgatttggc gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt
    64441 ttttggttga taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca
    64501 cctgaccata cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct
    64561 tccccttctg ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg
    64621 ttgatcattg cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta
    64681 cgacttgttt ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa
    64741 aagtatagtc ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat
    64801 ttttgatgga aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta
    64861 tatcccctga taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg
    64921 caacgatata ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga
    64981 aagattttct tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt
    65041 gaataatttg aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa
    65101 cgaaaaactc gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag
    65161 ttcttgaaag gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta
    65221 gccaaaatga gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc
    65281 taatactcta agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc
    65341 taataaatga gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt
    65401 atgactgtat gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact
    65461 tgtttttccg ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt
    65521 tatcaaaaaa gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga
    65581 taaggtttct tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat
    65641 tctttgagta gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc
    65701 tccattaata atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg
    65761 tgtatgctcg ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg
    65821 aaatagatcc cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc
    65881 attgatattc attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa
    65941 aaatgtctaa ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc
    66001 ttattagttc acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt
    66061 tctatggttg ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac
    66121 tatatttttg ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat
    66181 gaaattttct ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc
    66241 aattcccagt atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac
    66301 gactgctccc tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa
    66361 aatcggacaa aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct
    66421 ccccctcttc tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg
    66481 ctttggattg atcaattctt tggcagtcag catgattgct gccttttatc acggagatga
    66541 acgagcctct atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac
    66601 atttgttgca ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat
    66661 cagcttacct attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact
    66721 tcaatcgcat ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag
    66781 ttatggtatt tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc
    66841 cacagtcctg acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat
    66901 catgcaattg gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca
    66961 tcgctatatg cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac
    67021 tgtgataaac ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt
    67081 attcattcct tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt
    67141 caatacttca atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt
    67201 gctctttctc tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag
    67261 tatcttgctg ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa
    67321 gacagtgata agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg
    67381 gaggtcgtgt aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga
    67441 ctttgagatc cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg
    67501 gccagcgtac tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt
    67561 tcttgatcaa ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa
    67621 tggacaaaaa catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg
    67681 tatctttgta ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa
    67741 atcagatatt ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc
    67801 gctttatgtc gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca
    67861 tccagtcact gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa
    67921 tggctgtgta gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg
    67981 agagtccgat gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac
    68041 aagaaaaatg aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac
    68101 tggaatggtt caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt
    68161 ttcaagcgat tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt
    68221 atgtagtgat ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga
    68281 tgcgtatcaa tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg
    68341 aacgatatta gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga
    68401 ctattcgatc ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga
    68461 atttttacgc atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac
    68521 gaatgaatta gggtttattt atgggagagc aaaagattgg atatttattg attggtctga
    68581 aatggataaa gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc
    68641 catgatcact tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac
    68701 tcatctctat caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga
    68761 tgaagaactt ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca
    68821 tgcgaatatt ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt
    68881 aaagaatgtc ttattgaatg acactatcac acagattact actccatatt tcaaattcta
    68941 tgaacaagat gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga
    69001 ttattgggga gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac
    69061 tcaaacagga aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca
    69121 tgcatgggga gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac
    69181 aaagccaggt tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa
    69241 ttgtacgcta cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt
    69301 gattacttct gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct
    69361 agcaaaacag ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact
    69421 gcttgcgaca tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt
    69481 ttgaagtttg cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga
    69541 aagctagata atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga
    69601 ccgtattctc ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat
    69661 gtagaagaag cgattttaag atctcctaat actggaagga taaataatgg cagccaacta
    69721 atcaataagc tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg
    69781 ccgaagacac cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact
    69841 gtagtttgca agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa
    69901 aagccaatta atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt
    69961 ttagaatcgg gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac
    70021 accattcgaa ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc
    70081 agtgcaaaga ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca
    70141 gctaacaaag caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa
    70201 tctcgcaaaa agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt
    70261 ccaaaaaact ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac
    70321 gcaacttgat tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata
    70381 gttaatagtg ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac
    70441 cccgcgctta ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta
    70501 agaaaaatga atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact
    70561 agcatgatca aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca
    70621 ttgctaggaa ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt
    70681 agtgaattac tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg
    70741 atagttcctt ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca
    70801 atcaaagaaa cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta
    70861 tccattgata gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt
    70921 ttcttttact tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat
    70981 agaagttagc gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga
    71041 tacacttcca acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc
    71101 aagtgcttgt ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac
    71161 tgcaaaaatt ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg
    71221 ttgatgcgtg taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg
    71281 ccaaagcccg aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg
    71341 gatgggctgg attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta
    71401 cttcgtttac gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga
    71461 taaacgcacc atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt
    71521 tattttctga taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt
    71581 gatccgataa ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct
    71641 ccttcttttc taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt
    71701 ctacgccttc ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag
    71761 gcattgaaaa tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt
    71821 ccaagtcagt catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga
    71881 gaggtacagt agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag
    71941 ctgaattagt ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga
    72001 tcaatgaaga gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt
    72061 cttctgcgtc cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt
    72121 cttcagggat gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt
    72181 cgtagatgga agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag
    72241 aataggaaat acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac
    72301 gaaacgaagc aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac
    72361 attccagcaa aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc
    72421 aaagagagaa aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata
    72481 cttgtcgact aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt
    72541 ccatggatgt ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca
    72601 ttcgatttgg tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa
    72661 ctttggcgta ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag
    72721 caagtgactg catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg
    72781 accctagcag cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta
    72841 attcatttct tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat
    72901 cttgaaaatg aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat
    72961 ctcttcgctc ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg
    73021 cccagtcgat tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa
    73081 aaggagttaa ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt
    73141 ctagactgat ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt
    73201 tcacctccta cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa
    73261 cttacagttg gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa
    73321 aaaagcaggt gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga
    73381 tcatgtttat tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt
    73441 tcgatatctt tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt
    73501 gaaccagata aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat
    73561 tcttctttgt cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt
    73621 ttaggggcca ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg
    73681 gttcctccta gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa
    73741 acaaacccga ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat
    73801 tccttcttcc aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc
    73861 gccattaact aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga
    73921 aattactttt tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg
    73981 ttcgggcaaa tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc
    74041 ttcttgtcga tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact
    74101 tggacgttct ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa
    74161 aaaatccaga tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata
    74221 gctgatatca tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg
    74281 tccatataaa tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct
    74341 gaaataatca taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac
    74401 tttttggact gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag
    74461 acctttttct tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg
    74521 tttctctact agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa
    74581 ttgactaggt gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag
    74641 ctgatcagta tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat
    74701 ttttttgata ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt
    74761 actctttcca ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg
    74821 ttttcagtat atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag
    74881 ttcagaaaaa cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa
    74941 aatcaaaaag atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga
    75001 tcatgcttat tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga
    75061 ttgtcgatta catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag
    75121 caaaagctac gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca
    75181 gcggatttaa agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt
    75241 tcaatcaaac gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg
    75301 caaagaaaat ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac
    75361 atgaagattt agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg
    75421 caaaaggtga ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata
    75481 agtttttgat catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta
    75541 gcgaggattg cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa
    75601 tgacctattt gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag
    75661 aaatggccaa acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta
    75721 aagtagcgac tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag
    75781 tgatatattc tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga
    75841 tgcaaaaagg ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat
    75901 aagaaagata gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt
    75961 ctttttcaaa cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat
    76021 gaggaatagc taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag
    76081 gaggaaaatc agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga
    76141 tactttctgt acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac
    76201 aagtaagcga tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg
    76261 ccctttttcg attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac
    76321 ttgataagcg agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt
    76381 cggtaagtct gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg
    76441 tgtattttct ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag
    76501 cagcacagta ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa
    76561 aataccgatc gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt
    76621 agtaaaggga tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt
    76681 catgttttct tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata
    76741 atgagagatg atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc
    76801 tagtatccct attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt
    76861 ctttggtttc atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca
    76921 tttgcgtaat ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct
    76981 tgcgtctagc aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca
    77041 catagattcc cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt
    77101 caggagaaat caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac
    77161 ggtttgttaa tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat
    77221 attttagacc tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt
    77281 ctttcgccga tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg
    77341 tgtcactatc ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa
    77401 cgtcaccatc tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg
    77461 tttgatcatt tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat
    77521 ctgctttttc gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg
    77581 aaggaatata attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat
    77641 aatttactgg gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag
    77701 aatcattatc aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact
    77761 tgttttgcgt tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt
    77821 gaattttttc accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt
    77881 tagacgtatc atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa
    77941 aaggcgtgat tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta
    78001 aattatattt tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct
    78061 cggcaaagtc attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg
    78121 tttgatcagt tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc
    78181 ttggtgttcc ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg
    78241 cagtaccgat ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga
    78301 gatggatagt atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca
    78361 tctcatatac agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt
    78421 cttctttgat cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga
    78481 catttccatc agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga
    78541 ctgcttgttt tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag
    78601 tgacgttata aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca
    78661 tttctttccc gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa
    78721 ggatgacttg agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga
    78781 gtaatggtaa aataaggaaa aggactccta acataacgtt tattttttta tgatttttca
    78841 ttttttcact cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat
    78901 accaacaacg cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct
    78961 attttctttt ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa
    79021 agttgaaatg gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat
    79081 ctcttttaat cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc
    79141 ggaaatcagg tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg
    79201 ctggaggtaa tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc
    79261 tggtttattt gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt
    79321 tgtactgaca ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg
    79381 tgctgtactg cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc
    79441 caacgaaggg atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc
    79501 ttctgtcagc ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc
    79561 ttcatccgca ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt
    79621 cgtatgcaag gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa
    79681 taccttctca aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat
    79741 agttgaaagt ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc
    79801 cttttctttg acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt
    79861 ttcttgatct ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt
    79921 agttgttgct tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg
    79981 tgcttcttct accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata
    80041 aacttggaat gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc
    80101 gtcatttgga tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat
    80161 ctgaaccatt tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa
    80221 caagaagaga cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct
    80281 tttttcaaaa aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt
    80341 cctaaacggc caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct
    80401 aaagaaatct ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc
    80461 acttgaattg acccatcttc gtgtataact atagtaactg gctcttttag accttggtat
    80521 ccttctggtg taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga
    80581 aaggtatctg tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt
    80641 ctgaattttg ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca
    80701 aacgctttta gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt
    80761 tttcccgtgt aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa
    80821 gtatcatatc cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa
    80881 tcaaaactgg caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca
    80941 tttgcttctt ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc
    81001 ccttgcatcg catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc
    81061 aactggatcg tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca
    81121 gaagcgatct tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt
    81181 gtttctgtca gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag
    81241 gttccgtcgc catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca
    81301 gcgccaataa gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt
    81361 gtgaattgtt tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt
    81421 acaggtaatt ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt
    81481 agtgataata tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca
    81541 cgttcccacg tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc
    81601 caagctgcag catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga
    81661 tcagctagat tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga
    81721 gctttagcag atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa
    81781 gttgtcctgc cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg
    81841 attctcactt ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc
    81901 ttgatggtat ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca
    81961 ctcttgactg aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt
    82021 tgtccattgc ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata
    82081 tctgttgcat gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt
    82141 tgtcgcatac gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt
    82201 tggatgccaa gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg
    82261 gttgcgataa acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag
    82321 attcttgaga ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg
    82381 taataattgc tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca
    82441 ccatccgtca gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct
    82501 gcatctctta gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat
    82561 tttacttgat ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta
    82621 tatccttcgc ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct
    82681 gctaaagtat cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg
    82741 ttcatacttc ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt
    82801 acatttccac gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca
    82861 tactttcgta tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg
    82921 gtccgatcat taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt
    82981 ttattgcctt gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga
    83041 taagtgccct gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc
    83101 acagctggca aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt
    83161 gatgattctt cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt
    83221 tctgtctcat caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg
    83281 tggtaaagtg ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag
    83341 atttccaacg taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt
    83401 tccacatcac ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg
    83461 gtgttattcg catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact
    83521 tcagctgaaa ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat
    83581 ggcacaaagt ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct
    83641 gtggtcgtca tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat
    83701 tattaaaata aacaggcgtt acataatttt atttatttta atatgataat tattatacga
    83761 agggcgctaa agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat
    83821 tccttcgatt aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc
    83881 aggttgttgg aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct
    83941 gacaagtgga tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt
    84001 gtaaggtata tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca
    84061 caaaaattgg ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg
    84121 gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt
    84181 aaaacgattg agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag
    84241 ttgaaacaat gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt
    84301 tttcagttaa ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt
    84361 ttaagttact acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa
    84421 attatggtca agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa
    84481 tctttcttta ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt
    84541 gaatggaaag ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta
    84601 atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat
    84661 tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag
    84721 atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa
    84781 gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat
    84841 acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata
    84901 cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat
    84961 ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac
    85021 cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa
    85081 tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact
    85141 gctattactg aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg
    85201 aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc
    85261 gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt
    85321 ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat
    85381 tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca
    85441 attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa
    85501 actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca
    85561 tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt
    85621 tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca
    85681 ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt
    85741 taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc
    85801 caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc
    85861 tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat
    85921 tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc
    85981 tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt
    86041 tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca
    86101 tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta
    86161 gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta
    86221 agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac
    86281 cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg
    86341 aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt
    86401 cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa
    86461 agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta
    86521 tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg
    86581 aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt
    86641 tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta
    86701 atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt
    86761 acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa
    86821 ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata
    86881 agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa
    86941 agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt
    87001 cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa
    87061 aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat
    87121 gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc
    87181 tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg
    87241 tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta
    87301 ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat
    87361 cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt
    87421 tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc
    87481 gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg
    87541 tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa
    87601 tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt
    87661 tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat
    87721 caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat
    87781 agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt
    87841 tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa
    87901 attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc
    87961 ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta
    88021 aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg
    88081 caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg
    88141 gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt
    88201 tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata
    88261 gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt
    88321 tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct
    88381 ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct
    88441 tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt
    88501 tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg
    88561 attctgatac gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc
    88621 gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa
    88681 gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca
    88741 aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt acagatatta
    88801 aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt tgattctcga
    88861 acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa
    88921 acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt
    88981 aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt
    89041 gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa
    89101 aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa
    89161 aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa
    89221 atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg
    89281 attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt
    89341 tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta
    89401 acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa
    89461 gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg
    89521 gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc
    89581 aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat
    89641 ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa
    89701 atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat
    89761 aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact
    89821 ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat
    89881 caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta
    89941 atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt
    90001 aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac
    90061 aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt
    90121 cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg
    90181 cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt
    90241 cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc
    90301 caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga
    90361 tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg
    90421 gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt
    90481 tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta
    90541 atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt
    90601 cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg
    90661 gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg
    90721 gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat
    90781 cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga
    90841 caaattttcc atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag
    90901 ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac
    90961 ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt
    91021 caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat
    91081 acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc
    91141 cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt
    91201 ggtatattgt tacttataaa tttattctat aggagacgat tgttttctac aaatatttag
    91261 attggaattt gattaaaaca atttaattga aatttgaaaa catttatgtt aatgaaagtc
    91321 atattctaat aaaatgtcat ttgtttctat atgaattttt atgaaaaatt tcatataatt
    91381 tccactcgtt gtgatggttt aatttatcca atccatcaat aagcaaacaa cttatataaa
    91441 aaaggattaa ttctcaatat aattgctcct accatagaat aaatcttcat gtaaagtaat
    91501 gaagtaccaa ctatttctaa tacttcttta agaattatcc caacaatatc gttagacaaa
    91561 ccattattga ataaatggag agccaatcga ttctgaaaaa atgttttcta atttttcgga
    91621 aataaaagaa agagcctcct gccattagaa cgaaccaaat ccatttcatt gctcatgaag
    91681 taaccacata gctaaaaaga ggataaatca attaaaagat caaaagaatg actgcactaa
    91741 taaaataact aaaaatgtat cttcttactt catatctttt agctagtact tagttaaaaa
    91801 tttgcttcct aagatcaaga caaactgtct atcttcattt gatgaaatgc ggtaaaatat
    91861 agaaaatgaa cactattgga gggaatttgt aatggacagc aaaactagaa aacaactgca
    91921 agaaaagtta aagcaacgta cacacttgct cccttccgtc tttcaacagt ttttagaaga
    91981 aaatcgttct tccctttcat taggcagtcg ctatgaatac gcaaaagatt ttcatctgtt
    92041 tactgattat ttgagaaaag tattttctga agaagtggat gatcagctga ttatccattt
    92101 agaaaaacaa gattatcaga atttcttagc taaaatctat cgacatactc gttcgttcca
    92161 aactacagcg aaccaagaaa cggagcaatt gtttgaaaat agctattacg ggatttcaag
    92221 gaagcaatat tcattaaatc attttttacg atttttgtat caaaaaggac tgattgaaga
    92281 agagatttct cttttgggta caagtttacc ggaaacaacc aaagcagctc cgcgatatat
    92341 cgatgcagaa cttttcaaag attttgagca tttgtttatc ccgattgaag gctttcaaac
    92401 aagcagagaa gctagctttg aagaaaagaa tagaccgagg aaccttgcaa ttactgccat
    92461 tcttctgtat tgcggattaa agatccatga agtagtagaa cttaaacgta aagatgtatc
    92521 tttgaccaag cagattctta atctgaatag aaaagaaaac agactgaata aagtaccaat
    92581 accaaaagaa gcgatgccat ttctggagaa ctattttaag ttaacagcac aaaatacgga
    92641 cgaaaattct tttgtttttc gttcctcaca tgatcagcaa atttctcctc ggactattcg
    92701 ccaaattttg agcaaaatta cagtatataa aaacgtatcg ccagaactat gcagaaaaac
    92761 atatcgttat tatgcagaac tatatttaga tgatgctgac gcggtaaatt atttatgtgg
    92821 taatcgatac ctctcatccc attcttttca agatatctgg gaaaagatgg ttgatttttc
    92881 ttatcacgaa ttagcggcat tcgttcgggt ttcttccata taaaacaaat gtatgatctt
    92941 tttttcacaa taaactatgt tattatataa aggattatgt tacgattatc ttgaatttgt
    93001 atattactta acaaaagata atggagtgtg aatgatggag accataacgt attccccttt
    93061 gaacttattt acgaattttc aagaagctgc agctaacttt tccgagaccc cgattatctt
    93121 tgatgagcca ctttcagctt tcccagaatt aggacttgaa acaacttaca aaaaatgctg
    93181 cgaaagtgtg ttgaaacggg cttatcaatt ggctcatctt ggagtgaaat ccggagacaa
    93241 agttattatt tataagagtt ccgcttttga cacatacctt ctagctgttt ctgtgtctta
    93301 ccttggtgca gtgcctgtca tgacttctta tcatctacca acaacaacaa tggaagtatt
    93361 tatcgatcgt ttagaagacc ctttcatttt atttgatgat gagacaaaaa agcgagtgag
    93421 agaaatttca aatggaacaa aatcaaaaca gattc
//
LOCUS       DANJMA010000004        89995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000004 DANJMA010000000
VERSION     DANJMA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            255..485
                     /locus_tag="PWU31_000323"
     CDS             255..485
                     /locus_tag="PWU31_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1082899.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL"
     gene            485..826
                     /locus_tag="PWU31_000324"
     CDS             485..826
                     /locus_tag="PWU31_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1082900.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQLKSLDFKERRLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            1521..3602
                     /locus_tag="PWU31_000325"
     CDS             1521..3602
                     /locus_tag="PWU31_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1082901.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAERLAAEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFSLKLENDKQVRLETEIKEVKDGFLNRRERIFDLVLAIAYS
                     RNPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFFHYFPLAEIKKIEEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDYMITEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHIPFHLKEEALFENLYKHMAA
                     LIIRIKHAIQVMNPLKENIRTNYFPIHRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYQSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVDLVFSTFQLNYRNKPLLVIDPILTETNILVIEDFLKRNEELQRLKPNSHESTQLFT
                     QIIGVMEESGGRVDRIIYGKLEKLFEINNLEINKREIQPMLKDILTDSNIMIKEKAAT
                     WEETIERAAKPLVKENIVEERYVEAMIRAVNEYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NVEDFKQLLFQ"
     gene            3638..3940
                     /locus_tag="PWU31_000326"
     CDS             3638..3940
                     /locus_tag="PWU31_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1082902.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFSVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            3966..5255
                     /locus_tag="PWU31_000327"
     CDS             3966..5255
                     /locus_tag="PWU31_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1082903.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEEIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPILKAF"
     gene            5274..5681
                     /locus_tag="PWU31_000328"
     CDS             5274..5681
                     /locus_tag="PWU31_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1082904.1"
                     /translation="MFGFFKKKENEPKKRSIKEEKILSPEVTEKIQSEITQLNQEIST
                     TQEKPKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDVMRQIAKQVTIKGNK"
     gene            5704..6573
                     /locus_tag="PWU31_000329"
     CDS             5704..6573
                     /locus_tag="PWU31_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL1082905.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKGTFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            6575..7195
                     /locus_tag="PWU31_000330"
     CDS             6575..7195
                     /locus_tag="PWU31_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1082906.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SVGFNNLEERAKQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKTRLQELAERY
                     GYQVMTIRLTADFEVLWQRRYKRDREPDRHLSYIMDHYHYGDILEDRSKGTNHITKEA
                     FKKIITDRKYDEFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            7392..7997
                     /locus_tag="PWU31_000331"
     CDS             7392..7997
                     /locus_tag="PWU31_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1082907.1"
                     /translation="MNNKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTVIEEGHPTPKVDVRAYIRKDGKILLIEDKRTREWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVESTQLQAVFDTNKQKDIPQLFQYYKLVFACTIHDEDAKFI
                     ENNETSDMGFFSIEELPKLSEKRTTKKQLLILENKNQLYCD"
     gene            8133..8585
                     /locus_tag="PWU31_000332"
     CDS             8133..8585
                     /locus_tag="PWU31_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1082908.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLKKVCKNVSEDWNFVKKGNKRIY"
     gene            8622..9118
                     /locus_tag="PWU31_000333"
                     /pseudo
     CDS             8622..9118
                     /locus_tag="PWU31_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9136..9846
                     /locus_tag="PWU31_000334"
     CDS             9136..9846
                     /locus_tag="PWU31_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082909.1"
                     /translation="MKKVIVDANFWEVFPEATIEILSVSGIDNHVTEENEEMYHQLLN
                     SAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKISGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVSSVRHTLTKDESEAEL"
     gene            9988..10410
                     /locus_tag="PWU31_000335"
     CDS             9988..10410
                     /locus_tag="PWU31_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL1082910.1"
                     /translation="MESVYRQERKSTRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGIFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMDEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            10430..10654
                     /locus_tag="PWU31_000336"
     CDS             10430..10654
                     /locus_tag="PWU31_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082911.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRARNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKVKSHY"
     gene            10898..12211
                     /locus_tag="PWU31_000337"
     CDS             10898..12211
                     /locus_tag="PWU31_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1082912.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALSGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAMLLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGKLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            12539..14176
                     /locus_tag="PWU31_000338"
     CDS             12539..14176
                     /locus_tag="PWU31_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1082913.1"
                     /translation="MERKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDVIWLSPVYESPNDDNGYDISDYQAIMAEFGTMEEMEELIEEAKKRNIRIVMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGEEPNGLRSIFSGPAWQLDEQTGQYFL
                     HLYSKRQPDLNWKNKQVRQAIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSDPKRKELSMIFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDLYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDISEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEETPEHVFAYSRIYEDQRWLVVCNMSSEKQVFTVPRE
                     ALRIIVSNYPLKQLPDGKVELRPYEAFVMEVNKEKLI"
     gene            14324..14758
                     /locus_tag="PWU31_000339"
     CDS             14324..14758
                     /locus_tag="PWU31_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1082914.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRLLNKLADEAFRKTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FTKEVDGSWEELHQSYKAVLGNEASKELAEIMNEATDKLQKK"
     gene            14861..15952
                     /locus_tag="PWU31_000340"
     CDS             14861..15952
                     /locus_tag="PWU31_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL1082915.1"
                     /translation="MSQFDQSIILPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIVQIF
                     HGGRMTSRATLSGEQPVSASAVAAERPDAETPRALSENEIKETIQAFGEATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIDELLTTVFSTVEKYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRETKLDYLHVSLNAYDRRARSDKYSDQSI
                     LEAVHDTIQGKIPLIGVGGIRTRKDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            15982..16659
                     /locus_tag="PWU31_000341"
     CDS             15982..16659
                     /locus_tag="PWU31_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL1082916.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYKDEDFRNRALGNTLRPDDVDAKDYAAVYYAGGH
                     GTMWDFPEATDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGADYQSGPAFSEVVQIDGRLLTGQNPQSAHQLGEV
                     VVEALKH"
     gene            17004..18299
                     /locus_tag="PWU31_000342"
     CDS             17004..18299
                     /locus_tag="PWU31_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1082917.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLIPTSEIYLIEKQSKLGWIPSGF
                     NLIVKGKVTSEKQLSWITKEELIDRYRIHVYTEKTVIGLEEKKVVLAGGDKLDFDRLI
                     LATGSNQNFRNVPAEARFIHPVKKIQNIAALQQKIMDAKKIAIIGAGQAGIEIAEGLA
                     SGKTQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGISLFLEEQVVSLSENETAVVET
                     ERRRENYDLVLLANHSRPNNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSATKDQGTYRPVGNQWFGYFLGSVGMTEE
                     ESIFYPQKIETGYAEYKLAAINQSKVKVKFLFDSEKKILGAQLQSRKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPNMVWSKVANTAYED"
     gene            18289..19749
                     /locus_tag="PWU31_000343"
     CDS             18289..19749
                     /locus_tag="PWU31_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1082918.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKTSF
                     DQYLEDIELIGRMMGKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLGRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYETLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLEKLQETAMQQLPRKSTEDSALSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLNRELRGVSGIEIESYEEGKNYDLVITF
                     CQRNKQQNEYYLSEFASPYDIIRLKRRIEMLKKEKN"
     gene            19886..21382
                     /gene="glpK"
                     /locus_tag="PWU31_000344"
     CDS             19886..21382
                     /gene="glpK"
                     /locus_tag="PWU31_000344"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL1082919.1"
                     /translation="MTEPTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQDILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEAAA
                     KRSTNEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            21396..23219
                     /gene="glpO"
                     /locus_tag="PWU31_000345"
     CDS             21396..23219
                     /gene="glpO"
                     /locus_tag="PWU31_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL1082920.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQAAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPM
                     LLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LADIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVRELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMTPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAEYFGWTEEEKVQQTKRLEALIAESDLRELKGEK"
     gene            23220..23936
                     /locus_tag="PWU31_000346"
     CDS             23220..23936
                     /locus_tag="PWU31_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1082921.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            24242..26170
                     /locus_tag="PWU31_000347"
     CDS             24242..26170
                     /locus_tag="PWU31_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL1082922.1"
                     /translation="MNNEMISQLQFDQIKKEIQARAIGNYSKKRISKMTTSSNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLVLSPADLIEYADFLRSSRMI
                     TKFFDKNQYQAPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQII
                     EKEIQSKLLKFLRHPKNKEMIQEAIIVQKGECYTIPIKASYKNKVDGTIIDESNKGTT
                     VFIEPTVVSKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEAIDLLIETMTVLDIIF
                     ARAKFSREINGITPKINKSEHIVIKQGRHPFLPDHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEVFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FARDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMAMSMKLIQQAERYLE
                     EKAYRTQKKEFPPKTAGINRKDNKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIAV
                     YLEKEMIRVPRQRVRLVRSAENLYPTGYDLDSLFVDFKTRKQQRDLERGSKKAHKALV
                     KEMRKRQEERRAKDENNK"
     gene            26292..26846
                     /locus_tag="PWU31_000348"
     CDS             26292..26846
                     /locus_tag="PWU31_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL1082923.1"
                     /translation="MKISTNWRKEIQTIPNLLSIFRILLLPIYLYFVLRQSFYVAGVV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(27007..27321)
                     /locus_tag="PWU31_000349"
     CDS             complement(27007..27321)
                     /locus_tag="PWU31_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL1082924.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDFANAEFLDLKEVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVKLQSQGYAYI
                     KP"
     gene            complement(27335..27595)
                     /locus_tag="PWU31_000350"
     CDS             complement(27335..27595)
                     /locus_tag="PWU31_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL1082925.1"
                     /translation="MEIENKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFENPDSNPEEQAAKLEQAIKMIIKK"
     gene            27765..28079
                     /locus_tag="PWU31_000351"
                     /pseudo
     CDS             27765..28079
                     /locus_tag="PWU31_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            28081..29736
                     /locus_tag="PWU31_000352"
     CDS             28081..29736
                     /locus_tag="PWU31_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1082926.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISDRENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHLEQ
                     YDVLVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKKRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQPITGKDALISLASPANRQGRQVADTISGISRRNQGSIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVVHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWQELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            29819..31954
                     /locus_tag="PWU31_000353"
     CDS             29819..31954
                     /locus_tag="PWU31_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1082927.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKRNELRESMDTAKVEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFILERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMNAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTILQLVSQLSAEEIEGEEAYFKRITKENVSEQAEHLRSKEYIDDLK
                     RSDPLFLDHPDFIRGLTKNGKTVLSKKTIKKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLKLFGELISDDSEQSIAAYARKLLQKNYRKITRQ
                     IEEMAWVDEHRLFERIYEKRYGSAYVWPHTRTVDEAVILLAIHHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLEILAEIL
                     ASLKGEKAAIITQTEEETQTLQESIKKSSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DRDEQRRRRQMYTAISRGMKSIVVLERET"
     gene            32128..32637
                     /locus_tag="PWU31_000354"
     CDS             32128..32637
                     /locus_tag="PWU31_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082928.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFYLYAFLLEKKMLYFFIGFFALIFFSFLIISFVLTRWNNRNL
                     YRKVNKSFEYEDEELKKRQELLEEMYTERFGTKEERETVRFYSVSEEKNLDTTLIADL
                     YKENGVELK"
     gene            32634..33938
                     /locus_tag="PWU31_000355"
                     /pseudo
     CDS             32634..33938
                     /locus_tag="PWU31_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            34034..34903
                     /locus_tag="PWU31_000356"
     CDS             34034..34903
                     /locus_tag="PWU31_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1082929.1"
                     /translation="MGKVMKKRMKPLIWPIAVSGIFFFGHTAFAETSEPAQTEPSTTI
                     SSSDAENGTSETEMTSNVPTDNSGETISFSEDTSTNSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKAGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLDQVAETASGKLSQTQADITAASQAIHGKISEAQTVADDFEAKKADLAAS
                     FQRIKDILHSAGEKSVASGTTTETVQQTTEPSESTSQTLPKTNEKTASVNYSFVGTGV
                     LVAAAYGIIRKKK"
     gene            complement(35014..35334)
                     /locus_tag="PWU31_000357"
     CDS             complement(35014..35334)
                     /locus_tag="PWU31_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative heavy metal-binding protein"
                     /protein_id="HDL1082930.1"
                     /translation="MITTTTNSVEGKTVVAYKGIVFGEVITGINVLKDLGAGLRNVFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            35436..35882
                     /gene="dhaM"
                     /locus_tag="PWU31_000358"
     CDS             35436..35882
                     /gene="dhaM"
                     /locus_tag="PWU31_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL1082931.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            35857..36867
                     /gene="dhaK"
                     /locus_tag="PWU31_000359"
     CDS             35857..36867
                     /gene="dhaK"
                     /locus_tag="PWU31_000359"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL1082932.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGVALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVFVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            36896..37501
                     /gene="dhaL"
                     /locus_tag="PWU31_000360"
     CDS             36896..37501
                     /gene="dhaL"
                     /locus_tag="PWU31_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL1082933.1"
                     /translation="MKLTVQEIQEWLSQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQTDHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIEQAKEHTK
                     DLVAKKGRASYLGERAIGHIDPGAASSAILFTTLLNVIKTS"
     gene            37627..38286
                     /locus_tag="PWU31_000361"
     CDS             37627..38286
                     /locus_tag="PWU31_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082934.1"
                     /translation="MDLMKEDYQLFDRDNYAFKQLKEIHTPDEVEQIKQEYKAHWQKW
                     KEIQLQTAALLPDTYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSDTREGSVEGYNQSLSLLQEWSTQVAIEEYYIWPQPENELEDHLPL
                     SVYLSDKSKQEELRETMGNRTFQLGKLFFSPNEYTNIEEKTAEALKELAPLYHAIKNK
                     L"
     gene            38667..39182
                     /locus_tag="PWU31_000362"
     CDS             38667..39182
                     /locus_tag="PWU31_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082935.1"
                     /translation="MKKIIFRGVIVIIALSIGGKLLMDRREKDNEELRTIQTDLANYL
                     YNHYEISTVDEKREKEIFKEFNQGKGDMTEQEFFERLDSITEYMDIEKIEFTGFSVGP
                     MKGLVVGFIINDVYPDETTLDTRSAETNKWLYSFNTGNTRNSYVLTKKNSSTDEKMPE
                     ENIIYYDKGVK"
     gene            39182..40540
                     /locus_tag="PWU31_000363"
     CDS             39182..40540
                     /locus_tag="PWU31_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HDL1082936.1"
                     /translation="MDQNKEFSKAVYGVETGKVKVNEKIVEDEYLVIDVIDTDKHTIG
                     KEKTPKQNGMQAMVVAEIKDEYKSKKQNQLQQISDYPKSVIKKDLTIAYAGTKNWQDW
                     KTNIREVGFEDKHDNGAFQSALAYADAIEKTYSVKDGYTISTTGHSLGGAQAIYVAVL
                     KGYHGFTYAAAGPGLSEKQLMNYEGQIINLYDTSDIVTSGWLTGGKGQLPFHSFGIDN
                     AGWKNYGHDLNQFNLDKNGNYIDKYGDIVIYSDQHGGVALEQTLLAQQIIKNKAMIRQ
                     METYGEKNQWEKDRISQLKEENKWLQLQISAFSKLVTWRKRFTASSGGLSTNEQIYLD
                     DQQALMIVKHAASVFNQAMEQVLVIYQRGIRDLEQLWADGLAMVRRQTPLLSQNEMLD
                     ALREVGCTKQTIVDEPTQEFREKIFKIKQLSAEFSTLAKEIEAKINELVQRDRDLARQ
                     LF"
     gene            40557..40868
                     /locus_tag="PWU31_000364"
     CDS             40557..40868
                     /locus_tag="PWU31_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082937.1"
                     /translation="MEKENQIHETYRKERLQLEDQEDQLRQMQKNMQQLAETTYSNIR
                     FSVRSFECPKDSLYFAQKELRRLEERFSHELMQKRKKIYDQQDEVERRYRADLQRLNK
                     K"
     gene            40887..41150
                     /locus_tag="PWU31_000365"
     CDS             40887..41150
                     /locus_tag="PWU31_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082938.1"
                     /translation="MIVLEEKIWEDYQKEIQKISARALGIAMTQDKVTKCKKAKQAAE
                     VEASTAKKAVSSATTGLEKAVKGRFGKKVTAAFSKKSRMLVSF"
     gene            41506..41705
                     /locus_tag="PWU31_000366"
                     /pseudo
     CDS             41506..41705
                     /locus_tag="PWU31_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41740..42399
                     /locus_tag="PWU31_000367"
     CDS             41740..42399
                     /locus_tag="PWU31_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1082939.1"
                     /translation="MRHEGLCVSLVPLFNHLEIEDQQKIHSLVTHRKVEKGEQILAPN
                     GESQLVIVASGSMKVYHLSASGKEQLLRVVEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICVLRQKDFSELLLHYPALSLKLLAINAEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKNHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRENREVWIIDKEQLED
                     L"
     gene            complement(42478..44088)
                     /locus_tag="PWU31_000368"
     CDS             complement(42478..44088)
                     /locus_tag="PWU31_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL1082940.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETMQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAETILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSTGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAV
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNLLLAILFSILSILISSGALGLSALASMKDAKDMDLLLFLKAGFNFLPAVLVFIGM
                     SGFLFGWLPRMGKFAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALIVLGAIGYRRRDLVEG"
     gene            complement(44101..44982)
                     /locus_tag="PWU31_000369"
     CDS             complement(44101..44982)
                     /locus_tag="PWU31_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082941.1"
                     /translation="MAEIVKVTHLKKNFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGNAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALSVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEADNSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45235..45834
                     /locus_tag="PWU31_000370"
     CDS             45235..45834
                     /locus_tag="PWU31_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1082942.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAESAQVS
                     KATIFKYFGSKEGLIYEVFFTFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTCYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(45904..47205)
                     /locus_tag="PWU31_000371"
     CDS             complement(45904..47205)
                     /locus_tag="PWU31_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL1082943.1"
                     /translation="MEKKQKIILGATSALTVVILILSLFFVIRAYTKKEPIIPEKKIT
                     EKTKEPMDDKQQLVTQAKNLAQSYDYDQAIALLEKNSKNGSAETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQSQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKENDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIEEAKKVADQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGADFY
                     RNARINIDGIRFQSDLDGQNPILDPFINVHDVYDKEARESA"
     misc_binding    47467..47672
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            47777..49033
                     /locus_tag="PWU31_000372"
     CDS             47777..49033
                     /locus_tag="PWU31_000372"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL1082944.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EELAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFVKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            49324..51201
                     /gene="tdc"
                     /locus_tag="PWU31_000373"
     CDS             49324..51201
                     /gene="tdc"
                     /locus_tag="PWU31_000373"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL1082945.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            51444..52865
                     /locus_tag="PWU31_000374"
     CDS             51444..52865
                     /locus_tag="PWU31_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1082946.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            52975..54342
                     /gene="nhaC"
                     /locus_tag="PWU31_000375"
     CDS             52975..54342
                     /gene="nhaC"
                     /locus_tag="PWU31_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1082947.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFWGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(54623..55642)
                     /locus_tag="PWU31_000376"
     CDS             complement(54623..55642)
                     /locus_tag="PWU31_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1082948.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(55664..56671)
                     /locus_tag="PWU31_000377"
     CDS             complement(55664..56671)
                     /locus_tag="PWU31_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1082949.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(56664..57329)
                     /gene="pgmB"
                     /locus_tag="PWU31_000378"
     CDS             complement(56664..57329)
                     /gene="pgmB"
                     /locus_tag="PWU31_000378"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL1082950.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(57322..59616)
                     /locus_tag="PWU31_000379"
     CDS             complement(57322..59616)
                     /locus_tag="PWU31_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL1082951.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            59876..62041
                     /locus_tag="PWU31_000380"
     CDS             59876..62041
                     /locus_tag="PWU31_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1082952.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDRVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPVFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            62345..63178
                     /locus_tag="PWU31_000381"
     CDS             62345..63178
                     /locus_tag="PWU31_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL1082953.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(63280..64980)
                     /locus_tag="PWU31_000382"
     CDS             complement(63280..64980)
                     /locus_tag="PWU31_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082954.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            65487..66647
                     /locus_tag="PWU31_000383"
     CDS             65487..66647
                     /locus_tag="PWU31_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1082955.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            66663..67106
                     /locus_tag="PWU31_000384"
     CDS             66663..67106
                     /locus_tag="PWU31_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1082956.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(67710..68240)
                     /locus_tag="PWU31_000385"
     CDS             complement(67710..68240)
                     /locus_tag="PWU31_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL1082957.1"
                     /translation="MKEFKELEDFIGTHSIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            68345..68896
                     /locus_tag="PWU31_000386"
     CDS             68345..68896
                     /locus_tag="PWU31_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1082958.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(69049..69522)
                     /locus_tag="PWU31_000387"
     CDS             complement(69049..69522)
                     /locus_tag="PWU31_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1082959.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIVNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            69660..71132
                     /locus_tag="PWU31_000388"
     CDS             69660..71132
                     /locus_tag="PWU31_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1082960.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            71129..71839
                     /locus_tag="PWU31_000389"
     CDS             71129..71839
                     /locus_tag="PWU31_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL1082961.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            72191..72475
                     /locus_tag="PWU31_000390"
     CDS             72191..72475
                     /locus_tag="PWU31_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL1082962.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(72756..73175)
                     /locus_tag="PWU31_000391"
     CDS             complement(72756..73175)
                     /locus_tag="PWU31_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL1082963.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(73177..73761)
                     /locus_tag="PWU31_000392"
     CDS             complement(73177..73761)
                     /locus_tag="PWU31_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL1082964.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(73808..75283)
                     /locus_tag="PWU31_000393"
     CDS             complement(73808..75283)
                     /locus_tag="PWU31_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL1082965.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAKFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            75464..75775
                     /locus_tag="PWU31_000394"
     CDS             75464..75775
                     /locus_tag="PWU31_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082966.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(75890..78226)
                     /locus_tag="PWU31_000395"
     CDS             complement(75890..78226)
                     /locus_tag="PWU31_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1082967.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            78368..79036
                     /locus_tag="PWU31_000396"
     CDS             78368..79036
                     /locus_tag="PWU31_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1082968.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            79039..80082
                     /locus_tag="PWU31_000397"
     CDS             79039..80082
                     /locus_tag="PWU31_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1082969.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(80319..81200)
                     /locus_tag="PWU31_000398"
     CDS             complement(80319..81200)
                     /locus_tag="PWU31_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1082970.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            81359..82087
                     /locus_tag="PWU31_000399"
     CDS             81359..82087
                     /locus_tag="PWU31_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL1082971.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            82243..82560
                     /locus_tag="PWU31_000400"
     CDS             82243..82560
                     /locus_tag="PWU31_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1082972.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            82553..83161
                     /locus_tag="PWU31_000401"
     CDS             82553..83161
                     /locus_tag="PWU31_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL1082973.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            83216..84007
                     /locus_tag="PWU31_000402"
     CDS             83216..84007
                     /locus_tag="PWU31_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL1082974.1"
                     /translation="MLTGGLLAGATYTLGAQKCLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            84145..84642
                     /locus_tag="PWU31_000403"
     CDS             84145..84642
                     /locus_tag="PWU31_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL1082975.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KKLKK"
     gene            84685..85556
                     /locus_tag="PWU31_000404"
                     /pseudo
     CDS             84685..85556
                     /locus_tag="PWU31_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
     gene            85732..86094
                     /locus_tag="PWU31_000405"
     CDS             85732..86094
                     /locus_tag="PWU31_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1082976.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            86103..86855
                     /locus_tag="PWU31_000406"
     CDS             86103..86855
                     /locus_tag="PWU31_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1082977.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            86869..88671
                     /locus_tag="PWU31_000407"
     CDS             86869..88671
                     /locus_tag="PWU31_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1082978.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYRVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            88948..89988
                     /locus_tag="PWU31_000408"
     CDS             88948..89988
                     /locus_tag="PWU31_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1082979.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        29218 a        15793 c        19005 g        25979 t
ORIGIN      
        1 ctagatgaac tagcaagttt gattgcagac tgtagacaaa ggtatccctt gcctctttac
       61 gaagagtgta ggttgttact tttgtctatt tttgtataga aatagaaaac atttgtctcc
      121 atttccaagc tgctaatttt tcaaagtgca ccatttctca aaatgctttt gatcatttct
      181 ccatctagtt tacaaagggt acttttgcgt tacttagtgt tgtgtagtat acttttactg
      241 aagaggtgat gatgatgctt gttacaaaat ctagattaca aggaagttct gtagtggtta
      301 ctttaccttc tgataacgga aagaaacctt cagaaaacca agaatatatc gttgtatatt
      361 ctgacgatgg gacaattacg ttagttccaa aaattgaaga tccatttagt ggtggagaag
      421 aggccgaata ttatgaaaaa gacgaatggg aagatttaac tcctgaaggg agagaaaggt
      481 tatagtgtca gaagaaagaa tttatattcc taaaaaaggt gatattgtgt ggattgattt
      541 tgatccttcg ataggaaaag aaattcaaaa aagacgacca ggactagtcg tctctcgtta
      601 tgagtttaat cgtaaaacaa tgttttcggt catatgtccg attacttcca cgataaagaa
      661 tttaccgaca cgttactcat taccagaaga gctagatata aaaggacaag tacttatttc
      721 tcaattaaaa tcacttgatt ttaaggaacg aagactcaaa aaaatagaaa acctgccatt
      781 gcaagacatg gctaaaattg atcagattat acaatatatg ttttaagcgt tgtaactaaa
      841 ctctacaata tttttatatg ctaaacttaa caaaattttt tgacatttaa aaagaaaagt
      901 gacaataaaa aatagcatct aaatagcaat gacccccaaa tttaactctt ggggggacat
      961 ttcaaaaggg ataagaaaac aaacattctc gtcgtatctg actagtgaaa aagatggcag
     1021 gccgatttta ttcagccgat tatgctggat tgacaggaaa tatttatgaa tggagctgtg
     1081 acaaaaattt tgtcgcagct cctttttctg aataaacggt gttccagaga tagcctatcg
     1141 gcaataaggc acgattttgt aaaatatggg aagctatttt tcaaaatcgc ttcttatcgc
     1201 tcaaggctga acctctccgt cacaaccttt ccactaaatg gtattcaaaa gcttacattt
     1261 acggtaataa gctcaaccaa acgaaaaata ggaagcacta tttttgtttg gctgttcttc
     1321 ttacttgatg caggacagct ttttcatgac ctcttttttg taataatagg cgtatgtctt
     1381 tttcgttgag aagttgaaaa agcatacggc ttattttttg tttgctatgc ttactttctt
     1441 tccacttcaa gtggcagaaa tatctacttt ttattatgta agcggaacca tatacttgga
     1501 atcagaagga gatcatttct atgaaaacaa gaacaattga aatacttcac agaatcatga
     1561 cagcccaata tccagtaaaa gcggaacgtc tagcagctga attcaatgtc agtcagcgga
     1621 cgatccgtca ggaagttctt gaagcgaata tctggttgag tagtaaaaag cttccggaaa
     1681 tcagaacaat tcgcaacaaa ggtttttcgt tgaaactgga gaatgacaag caagttcgat
     1741 tagaaactga aataaaagag gtgaaagacg gctttttgaa tcggcgggaa cgtatttttg
     1801 atttagtttt agccattgct tacagccgaa acccgatcca tctaaacaaa aaagaagaag
     1861 aatttcaaat ctctaaaagt acgttagatg aagatatgag acgattacga gcaacactgg
     1921 tcagttatgg aatcgagatc gtcagttatg gaaagcaggg actggtttat aaaggttcag
     1981 aacgatctat ccgtacgatg atctatgatt ttatcaatca gaatctgggg atgaccgact
     2041 tctttatgaa tcaagatgca caactaacac cggctcaaaa aatctttttc cattattttc
     2101 ctttggcgga aatcaaaaaa atagaggaga tctattccaa taagttgtta aaacaagagg
     2161 atgatattta taaaaagcaa attctgcttt ttacattgat ctggatccaa cgaatcagga
     2221 aacatgaatt catctctgcc attaattgga aaaatacaga aataaaagac agcagcttct
     2281 ctgcatttat tgactacatg atcacggaat ttcaattgga caatgttcca caagtagaaa
     2341 gaaattatct tcgtttcact atcgagacgt tcaatgctag agatatctcc aattcgattg
     2401 agtgggtaca agctcaacta ctaacgatcc aattgatcca atttgtagag aacgagacac
     2461 acataccttt tcatctaaaa gaagaagctt tattcgaaaa tctctataaa cacatggctg
     2521 cactgatcat aagaatcaag catgctattc aagtcatgaa tccattgaaa gaaaacatcc
     2581 gtacaaatta ttttccaatc caccgcgcag tcactcattt tgttcctacg atcgaagaag
     2641 taatcggcgg caagatactg gaggacgaga tagcttttct tgtcatccac ttttcgacga
     2701 ttgccagtgt gatcaagcga gacctgaatt atgtttatca atcagttgtt gtctgtaatc
     2761 acggcatggc taccgggaat ctgttagcag aaaatctgaa agaaaaattt cctcaaatcg
     2821 agattactgc agttttaagt tcaaaagaaa tcgaattagt ggataaactg gatgtcgatc
     2881 tggttttcag cacctttcaa ttgaattatc gaaataagcc gcttttagtt atcgatccaa
     2941 ttttgactga gacgaatatt ttagttatag aagacttttt gaaaagaaat gaagaactgc
     3001 agcggttaaa acctaacagt catgaatcaa cgcagttatt cactcaaata atcggtgtca
     3061 tggaagaaag tggtggccga gtagatcgca tcatctatgg caagttggaa aaattatttg
     3121 aaataaacaa tctagaaatc aacaaaaggg agatccagcc tatgttaaaa gatattttga
     3181 cggacagcaa tatcatgatc aaagaaaaag ccgctacatg ggaggaaacg attgaacgag
     3241 ctgctaaacc attggtcaaa gaaaacattg ttgaagaacg ctatgtcgaa gcgatgattc
     3301 gcgcagtgaa tgaatacggg ccatatatcg tgattggcaa acacatggcg ctagcccatg
     3361 ctcgaccaga agatggggtc aataagttag gagtcagcgt agctacgatt gagcaaccaa
     3421 tcgatttcgg taatcctgaa atggatccag taaagattat tttttgttta gcggcagtag
     3481 attcctactc gcatctgaac atcatgaaag aactgatcga actgatcaac aatgaagaga
     3541 agttggatcg attgatcgag tgcacaaatg ttgaagattt caaacaactg ctttttcagt
     3601 agttgagaaa taataaatga tacgggggag aacaaagatg aacaaattaa acattttatt
     3661 cgtatgcggc gcaggtttag gaagtagttt tgcagcacag atgtctgctg aggatgtttt
     3721 gaacaaatta ggagtagaag ccaaactgga ccattgtgat atttcttctg cagtgtccat
     3781 gaaaccagat gtgatcatca cagcagaaaa ttttcgctcg cagttcgaaa aattctcagt
     3841 cgatcctaaa acagcgatga tctatttgaa aaatattgtc tcgaaaaatg aaattgaaga
     3901 aaaaatcacc ccagttctac aagataaggg agttatctaa ataaaaaaga aaagaggata
     3961 aaaacatggg cgttatcaat tttatcatcg aaaatatttt gactcaggca tcgatcacca
     4021 ttgcattgat tgctatgttg ggtctactgc tgcagaagaa atcagcagga caagtcattt
     4081 ctggcacgtt aaagacactc ctaggattcc aagtgttgag tgccggttcg agtatcatcg
     4141 ttggaagttt aacttatttc gggaaaattt ttactgaagg atttcatatg caaggaatca
     4201 ttccttctat tgagtctatc aatggtcaag caatgaatga tttaggttta ggtagagata
     4261 ttgcactgac gttcttagcg atttttgtat tcaatattat tttggctcgt ttcacaaaat
     4321 ggaagtacat ttttctaact ggtcaagcca ttttatggat ggcgacgatg acgacggtct
     4381 ttggttattt cgctggtctg cgagggatcg tattgatctt agtcggtggt tttatcggag
     4441 cttgtttcgc cattgctatg ccagcagtag cgcaaccaat tatacgtaag attacaggat
     4501 cgaatgatat tgctttaggg catttttgta cgattggata cttatttgaa gctggcgtgg
     4561 ctaaactttt tggtgagaaa ggtgaaaata aaaaatcgat tgaagagatc aaattgccca
     4621 cgcatttcga gtttctgcaa gatacgtatt tgtctgtcat ggtcgtgatg gtcccactat
     4681 atatcatcac tgttttgttt gcaggtgaac cattcgcttc tgaattatca ggagatcaaa
     4741 actatatcat gtttgccttt ttacaagcca tccaattcgt agtgggtgtg tatgtattgc
     4801 tagctggcgt acgtctactg ttaggtgaaa tcgtgccggc atttcgaggg atagctatga
     4861 aattagttcc tgatgcgatt cctgccttag attgtccagt tttctttccc tatagcccaa
     4921 atgctgttat tttaggtttc attacgacaa caatcggtac gattattgcc atgttcactt
     4981 tgccgatgtt cggcttagcg atgattttac caggtatgct aacgaatttc tttgccggag
     5041 gaacagcggg gatatttggt aacgcagttg gcggacgtcg aggagcgatc attggcggta
     5101 ttgctcatgg cttctttatt acattattgc ctgctttgct agtaacgatc tttaattcga
     5161 tgggattcat caatgctaca gcaaccgatg tagatacagt agccgccgca ctcttatacg
     5221 cttggatctt aagcccaatt ttgaaagcat tttaattgaa agtagggaaa atgatgttcg
     5281 gattttttaa gaaaaaagaa aatgaaccaa aaaaaagatc gataaaagag gaaaaaatcc
     5341 tctcgccaga agtgaccgaa aagattcaat cagagattac acaattaaac caagagattt
     5401 caacgaccca agagaagccc aaattagcgg aactttacga acaagttgga cttaaatttt
     5461 ctgaattgta tgagaacgat caagccatcc aatatttaga aaagagcttg gaaaataaac
     5521 agacgatcgg agatgggtat aaaaaactga tgagcctcta taatcagaag cgtgcagatg
     5581 ctgctcgtgc gggtgatgat caagggatcg attactacat gggcaaaatg gatgtgatgc
     5641 gacaaattgc aaaacaagta acgatcaaag gaaacaaata aaaagataag gtggcaataa
     5701 aaaatgtata caacattgaa agaagtaaca aaaacagcag aagagttgaa ttttacagtt
     5761 ggagctttta atacgcataa cctagaaatg cttcctgcca tgctaggcgc agcaaaagaa
     5821 atgggttcgc caatcattat tcaaacaagt gtcgacaccg caaaatatat cggtatgcat
     5881 gtgttagtca atgtaatgac agctattgca gaagaagaaa tggttgatgc agtcttgcac
     5941 ttagaccacg cccgagactt tgatgatatc aaagaagcaa tcgatagcgg atatacttct
     6001 gtcatgtatg acgggtccca tctgcctttc aaagaaaata tcttgaagac gaaagcagtg
     6061 gtagaatatg cccatgcaca cggtgtttcc gtagaaggag aacttgggac gatcggcggt
     6121 acagaagaag gaatccatgt cgctgaaaat gacaaagtat atacaagacc ggaagacgca
     6181 gtgaaatttg tagaagctac aggtgtggat gcattagcta ttgctatcgg tacgaatcat
     6241 ggacaattca aatcaaaaac agaagtgaac attcccttgc tgaaagaaat cgatgcagta
     6301 gtcgatgtcc ccctcgttat ccatggcgga acaggtgtga aagaagaaga ttatcccgaa
     6361 ttgatcaata acgggatccg caaattcaat gttggaacgg agcttttagt caactggaca
     6421 aaagtggcta aaggaacctt tagtgaaaca gaaatcaaca agtctcttcg ccataatgtg
     6481 attccagcaa atgaagcagt aaaagaaatt gtcaagcata agattggact gtttatgaac
     6541 ttggaaagtc caattaactt aggaaacaaa tgagatgaaa aagtatttca ttcttctagc
     6601 tggtagtcct ggtacaggaa aaacatatct tatgaataaa ctacgggaac gatttccaga
     6661 tatgtatgcc atcacgctcg atgaaatcaa ggaatattat gcagaatccg tcggttttaa
     6721 caacttggaa gaacgggcga aacaagaaag gacgaaagtt tatcctttct attacaaagc
     6781 acttgatctg tatatggaag caggaaagaa agtgattgtg tcagagtatc cttttagtga
     6841 taagcaaaaa actcgactcc aagaattagc agaaagatac ggatatcagg taatgacgat
     6901 tcgcctaaca gcagactttg aagtgttatg gcaacgtcgg tacaaacgtg atcgtgaacc
     6961 agatcgtcat ttgagctata tcatggatca ttatcactat ggcgacatat tggaagatcg
     7021 atcgaaaggg acgaatcata ttacgaaaga agcattcaaa aaaatcatca ctgaccgaaa
     7081 atatgacgaa tttcagttag gaacactata tgaatttgat gtaacagact atactcaagt
     7141 agattacact ccactattgg ataaattaga aaaagaaatc aatgacaata agtaacgaag
     7201 aaatggtttg atagtgacag gtaataaaaa agacatttgg tccccagagg agccaaatgt
     7261 ctttttgttc taaaatgttt tttcatattc ttttatttat tgccgatttc cttgtgttta
     7321 tttgccgtaa ttaggtcagt cttctatact tgattacata gtcatacatt tgctgaaaag
     7381 aagggaagag aatgaataac aagttggccc aatacaaaag actgctcgca ctttctcaag
     7441 ctggtctgta ttatggaaaa gatgtattcg ataaagaacg atttgaagag attcggacga
     7501 tctcattgga attgatcagt gaaataggaa aagaaacttt cgaagaaata aaagcactta
     7561 cagttattga agaaggtcat cccacaccaa aagtcgatgt tcgtgcctat atcagaaagg
     7621 acggcaagat cctactaatc gaagataaac ggacgagaga atggtcactg ccaggcggat
     7681 ttgccgaaat aggtttgtcc cctgaagaaa acgtgcggaa agaagtctat gaagaaacgg
     7741 gtttaacagt agaatctact cagctgcaag cggtttttga tacgaataaa caaaaagata
     7801 ttccgcagct ttttcagtat tataagcttg tttttgcttg tacgatccac gacgaagatg
     7861 caaaatttat tgagaacaat gaaacttccg acatgggatt ttttagtata gaagagctgc
     7921 caaagctatc agaaaaacga acaacaaaga agcagttgct tattttagaa aacaaaaatc
     7981 agctctattg cgattgagtt ttttcgtaaa agaagaaagc tgtttactct gcgagttctc
     8041 taaaaaagaa ctcgttcttt tattaaaaaa tagttgcatt tgcaacaaaa agaggtaaac
     8101 tagtcttaca aacaaaataa aggtggggtg aaatggctga aattcttcga gacattggaa
     8161 tgattgcccg agcgttggat tctatcagca atatcgaatt caaagaagtt gatttaacaa
     8221 gagggcagta tctttattta gtacgtattt gcgaaaatcc aggaatcatc caagaaaaac
     8281 tagcagaaat gatcaaagta gatcgtacaa cgactgcacg agcaatcaag aaattagaat
     8341 ccaatgggat gatcgaacgt ttggaagata aagaaaataa aaaaatcaag aaactttatc
     8401 cgactaaaaa aggagcagaa atctatcctt tcatcatccg agaaaacaac tactctaatg
     8461 cagtcgcatt aaatggtctg tctgacgagg aagcaaaaca attggaatat cttttaaaga
     8521 aagtatgcaa gaatgttagc gaagattgga attttgtcaa aaaaggtaat aaaaggatct
     8581 attgaaagga agaagccgtg aataaatacg aaattcgaga aatgacgcct tcagatgtag
     8641 acttactaca acaaatcagt atcgaaacat ttacagatac atttggaaaa gataattcgc
     8701 cggagcattt agctgcttac ttgagacaag cttatgcgcc agaaaaactt acagaagagc
     8761 taaaagagaa agaaactgat ttttactagt aagtatggac caagagcctg cgggttacct
     8821 gaaactaaat ataggaacga gtcaatctga ggaaatggat gatcattttt tagaaatcga
     8881 gcgaatctat gtacgaaaaa aatttaaaca tcaaggtatt ggtacatttt tactagatac
     8941 agctatcaaa aaagcaaaag aaaagcaaaa aacagccatt tggctcggtg tgtgggaaca
     9001 caacgatgct gcgctcgctt tctatcgcaa aagaggattt accaagtaca gtgaacatgt
     9061 tttttatatg ggagatgatc cccaaacaga ctatctattc atcaagcaat tattctaatc
     9121 aagaggtgtg aacatatgaa aaaagtaata gtggatgcca atttttggga agtatttcct
     9181 gaagcaacca tcgaaatctt atccgtgagt ggtatcgata atcacgtaac agaagaaaat
     9241 gaagaaatgt atcatcaatt attgaatagc gcggcaaaag aagcaagaaa ttaccttacg
     9301 gaagaaacat tcagccaaaa cgaagtcatt gctcagtggc gtcaggcttt taccacattc
     9361 aagactaaaa aaggtgcacg ttcttctatt gaagctttac tgaaacgtgc aaatcaagaa
     9421 cgggaatttt tcccaatcaa tccgcttgta gatatttata atagtatttc cttgtctttt
     9481 ggcgtacctt gcggcggaga agatatcgaa aagatttcag gaaatctgca tctaggaaaa
     9541 gcccaaggtg gcgaatcatt cctgccttta ggtgcagatt ccgatgctcc ggcacttccg
     9601 gaagaaatca tctactatga tgaacaagga gcaatctgtc gctgtctcaa ctggcgtgaa
     9661 gcccaaagaa cgatgctgac ggaagaaacg caagcagcgg tgctggtcat tgaatccatc
     9721 aatgctgaac aagcggcacg cggcgcacag gcaatcgaag cgttgaagga aaaaatcgat
     9781 caaacatttg gcgtgtccag tgtcagacac acactaacca aagatgaatc agaagcagaa
     9841 ttataaaaga atcgaaaaaa gaagagcggc attcattgcc gttctttttt tgtaaaaaaa
     9901 gtttgtggag gaaagcaagt ctagaaacta ttgctttatt tttcttgaat aggatcaaac
     9961 acaaggtgtt cgcgctttcg tgatataatg gaatcagttt atagacagga aagaaaatca
    10021 actagaaaga agggtcaaac gatgatacaa aaaacacgtg tgatgcttta tgtcaatgac
    10081 gtagagatga tttgccgatt ctttgtagaa aaaattggtg cagaaatttc agaaacgatc
    10141 gaattgccag aagaatttaa gagtattgtg ctgagtatct caaaagaatt agaattaggt
    10201 atatttccaa aagtatttgt gcagaagttt tcaccagaag tattgggacc gccaccttct
    10261 ttagtctttt ttacggatga atttgaaact atttacgaga acatggacga acctggagaa
    10321 atcacagaca acaatggcgt cttgactttc aatttttcgg accctgaagg caattatttt
    10381 gttataggaa aagctgagtc gtctgattga ataaaaagga ggaaaaacag tggatctaat
    10441 gggaaggcta gaaggttatt tgaagtatcg gcgagcaaaa catagtgaga agcttcgagc
    10501 aaggaatctc aaatcaggga aaaagaagac aaaaggctgg ggaaaaatgg tcgatacagg
    10561 ttttggcgga ctaaataacc taaatcctgt cgatacaaaa gaattcatca aagaagagca
    10621 gaaacgaaat ggtaaagtca aatcacacta ttgattttga tactagaatg aagaaacttt
    10681 tttaacacaa taaagaaatt cttttgatcc aaagtgaatg ttcatggact ttgagataaa
    10741 gggatttgaa cagatggaac gttcggattt tgcttaaaat ccgaattttt tgtttactta
    10801 tgttttgttt tagtatatga taaaacacgt tgttttatta atcaatcgaa attttaacgg
    10861 actttattta taatgttcgg atttaggggg atggaagatg aaggaaaaaa tactttccta
    10921 cttcgaaata gaaaaattga ataccacggt aaaacgtgag gtactggcag gatttacgac
    10981 atttatttca atggcctata tcttatttgt aaatccaact gtattaggcg cttctggaat
    11041 ggatgaagga gcggtgttca ctgcgactgc attagccagt gcaatcggct gtattttgat
    11101 gggggtatta gcccgttatc caattggaac ggcacctgct cttgggatca atgctttctt
    11161 tgcttattct gtttgtttag ggatggggat cccatgggaa acagcattgt ccggcgtatt
    11221 cgttgcttct ttgatcttta ttttgattac aatcttcaaa ctgcgggaaa tgatcattga
    11281 tgcgatccca gcagatttga aatatgcgat ttctggtggt atcggtctat ttattgcttt
    11341 ccttggtctg agcgaaggtg ggatcatcgt ttcgaatgaa tctacattag taggattagg
    11401 atcactcaac gttggttcta cttggttgac gatttttgga ttagtcgtca cggctatgtt
    11461 gcttgttcga cgtgtaccag gtggcatttt catcgggatg gcagctacaa cgattttagg
    11521 tctggtcaca ggtttgattc caatgccttc tgaaattgtt gctgcagcac caagtttgaa
    11581 accaacattt ttagttgcgt taaaacatgt aggggacatc aattctcttc aaatgtgggt
    11641 cgtagtacta acattcttgc ttgtgacttt ctttgataca gcaggaactt tagttggatt
    11701 agcaaatcaa gcaggcttta tgaaagacaa caagatgcct cgtgtaggga aagctttagc
    11761 ttctgattca acagctatgc ttgcgggctc cttattaggt acttctcctg ttggtgcatt
    11821 cgtagaatca tcagctggga ttgctgttgg aggacgctca ggaatcacag ctattactac
    11881 agggattttc ttcttgttag gtttgttctt ttctccatta ctgtcagttg ttacttcaca
    11941 agtaactgca ccagcattga tcatggtagg tgttttgatg gctcaatcat tacgtcaaat
    12001 cgaatgggga aaattagaaa ttgcgatccc ttccttcttg atcttgattg gcatgccttt
    12061 aacttatagt atttcagatg ggattgcatt aggttttatt ttctatccaa ttacgatgtt
    12121 agcggctaaa agaggaaaag aagtatctcc aatcatgtat ggtctgttct ttgtattcgt
    12181 gggattcatg tggatattga atgtgaaata aagaggttgt gaaatcagcg ttctggcctg
    12241 agtattagag aaaaaaacga aaaaatagtt cctcctattt tgccgttttt ggagctaata
    12301 ccgagggaca gctgagtgaa caccgtttat aaagaggttg tgaaatcagc gttcttccct
    12361 gcatcaatca atcctttcaa aaaacagttg aggaaatttc ctcagctgtt tttttgtttt
    12421 attctgttac cgataacaag gtgcaatgac tatttgaaag cgttacaatt tttggtagaa
    12481 gaaactaaac tagatgatta agcgaaaaat agaaacatcc atttatagga ggaaaatcat
    12541 ggaacgaaaa tggtggcacc attcagttgt ttatcaaatc tatccaagaa gttttcaaga
    12601 ttcaaatcaa gatgggattg gagacttgcg agggattatt tccagattgg attatctggc
    12661 atatttgggc attgatgtga tttggctttc gccggtgtac gagtctccaa atgatgacaa
    12721 tggctatgat atcagcgatt atcaagcaat catggcagaa ttcggtacga tggaagaaat
    12781 ggaggaactg attgaagaag caaagaaaag aaacatccgt atcgttatgg atctggtggt
    12841 gaatcatact tctgatgaac acccgtggtt cattgaggca cgtaaaaata aagaaaatcc
    12901 ttatcgtgac tactatattt ggcgtgatcc agtcaatgga gaggagccta atggattgcg
    12961 ctcgatattc agtggacctg cttggcagtt ggatgagcaa acagggcaat attttctgca
    13021 cttgtatagc aagcgtcaac ctgatttgaa ttggaaaaac aaacaggttc gccaagcgat
    13081 ttatcaaatg atgaatttct ggatcgacaa aggtattggt ggctttcgta tggacgtgat
    13141 cgatatgatt ggcaaagaac cggataaaga aatcacaagt aatggaccta aactgcatga
    13201 gtatcttcag gaaatgaatc aagcaacttt tggcgacaaa aatctgatga ccgttggcga
    13261 aacatgggga gcaactaccg agattgctaa actttattcc gatcctaaaa gaaaggaatt
    13321 atccatgatc ttccaattcg aacatatagg attagatcag caagaaggca aagacaaatg
    13381 ggatttgaaa cctttaagta tctctgaact gaaaaaagta ttatcgaaat ggcaaacttc
    13441 tttgggtgat gaaggatgga atagtttatt ttggaataat catgatctgc ctcgaatcgt
    13501 ctcacgttgg ggaaatgacg atttgtatcg aaaagaaagt gccaagatgt ttgctatctt
    13561 gcttcatatg atgaaaggca ctccttatat ttatcaagga gaagaattag gcatgacgaa
    13621 ttgcccaatt gatgatatct cggaagcaaa agatattgag acaatcaata tgtataacga
    13681 aaggatttct tctggattta caaaagagga aatcttggaa tcaatcaatg cgaaaggaag
    13741 agacaatgct aggacgccta tgcaatggaa cgcccgtgaa catgctgggt ttactactgg
    13801 aattccatgg cttcgagtca acccaaatta taaagaaatc aatgcagaag aagcgttagc
    13861 agatcaagat tctgtttttt atatgtacaa gaaattgatc gatttgcgta gaaaacatga
    13921 catcatcgtc tggggagact atgaattagt agaagagacg ccagagcatg tctttgccta
    13981 ttctcgcata tatgaagacc agcgatggct cgttgtttgc aatatgtcca gtgaaaaaca
    14041 ggtgttcact gttccacgtg aagctttaag gattattgtg tcaaattatc ctctgaaaca
    14101 gttaccagac ggcaaagtcg agttacgacc atatgaagca tttgtgatgg aagtaaataa
    14161 agaaaagctt atttgattga aacgtattac cgagaggttg tgacacattt tttgtcacaa
    14221 cttctttttc tcaaaactaa acagaggagg cggtcattac tccgtgtttt ttgacaaaaa
    14281 ataaaaatca tactatgata tgtacgccaa tggaggggat gaaatggaaa agccaattga
    14341 aaaagcgttg gatgaatgtc ttttctttag cgtgaaaaag ctagatcgcc tattgaataa
    14401 attagcggat gaagcgttta gaaaaacagg tctggcacca acttatggat ttattttatt
    14461 gatattaaaa gaaaaagatg gtataccgca aaaagatatt gcacaaatgc tctattctgc
    14521 gccttcaaca atcgctcgtt ttgttgaaaa attagaatat aaaggatacg tcaaaacagt
    14581 tagcgaaggg cgcttgtcat tagtttatct tacagaacaa ggacgccaat ttactaagga
    14641 agtcgatggt agctgggaag aactccatca gtcgtacaaa gcagtcttgg gaaatgaagc
    14701 aagtaaagaa ttagccgaga taatgaatga agcaacagat aagttacaaa aaaaataaaa
    14761 atttttttaa gcaaattagt tgcatgtgca actaaccgag tggtttaatg ataaatgatt
    14821 ctcgataggt taatcagagc gaattatggg aggaatgaca atgtcacaat ttgatcaatc
    14881 aattatttta ccttcaggag ttcaattgaa gaaccgattg atgctgtcgc ctatgacgac
    14941 acaactcagc ttcttcaatg gagtgatcac cgaagatgag atcacttatt ataaacaacg
    15001 ttctaaagga cttggtgctg ttattactgg tgctgcaaat gtccaagata tcggaaaagg
    15061 ctggccaggc gaactgagca tcgctcacga tgaaatgatt cctcggttaa gtgaactagc
    15121 taaagcaatc caaggagaag gtgcaaaagc gattgttcag attttccacg gtggacgaat
    15181 gactagtcga gctactctat ccggagaaca gcctgtatct gcaagtgcag ttgcagcgga
    15241 acgacctgat gcagaaacac cgcgagcatt aagtgaaaat gaaatcaaag aaacaatcca
    15301 agcatttggc gaagccacac gcagagcgat tgaatcagga tttgatggta tcgagttgca
    15361 tggagcaaat acgtatttga tccaacaatt tttctctccg cattcgaata gacgagagga
    15421 tcaatggggc ggtagcttag aaaatcgcta tcgttttatt gacgaattat taacaacggt
    15481 tttctcaacg gttgaaaaat atgcaaaaaa accattcatc gtagggtatc gtttctcgcc
    15541 agaagaatat gaaacaccag gtatccgttt tgaagacacg atctggcttc ttgaacgatt
    15601 acgtgagaca aaattagatt atctgcatgt ttcattaaat gcgtatgatc gaagagcccg
    15661 ttctgataaa tacagcgatc agtcgattct tgaagctgtc catgacacga tccaaggaaa
    15721 gatcccttta attggtgtcg gaggcatccg tactcgcaaa gacgtagagc aagtactgaa
    15781 atatgcagaa cttgcagcaa ttggacagca aatgatcgtc gatcctgact gggcagagaa
    15841 attattggaa aaccgtgagg atgaatttgt aacgaaacct tttgaagaag catataaaga
    15901 gctttatttg ccaagtccat tatataattt cttgaaagca cgttatcaat aactcaattt
    15961 ttatagaaag aggatggaaa aatgaaagta ttgatcgtat taacaaatgt agaaaagtac
    16021 gaaaagatcg agcgtcctac aggtttgtgg ttaagtgaac tgactcattt ttatgatgta
    16081 ttagtaaaaa atgggatcga tgcagatttt gtcagtccta aaggcggata tgtaccttta
    16141 gaaccgcaaa gtgttttgaa tatggatgaa atcgattggg cctattataa agatgaagat
    16201 ttcagaaatc gggccttggg aaatacctta cgtcctgatg atgtggatgc aaaagattat
    16261 gcagcagttt attatgctgg tggccacgga acaatgtggg attttccaga agcaacagat
    16321 cttgggaaag ttgcttcaca aatctatcga aatgaaggac tggtctcagc tgtttgtcac
    16381 ggtgttgtcg gattgttgcc agttacagat gaatctggaa agtctatttt agctggacgc
    16441 acagtgactg gattcagtaa tgaagaagag gaagccaatg gtacaacgaa tgaagtacca
    16501 ttcttagcag aagatgcttt gaaagaaaaa ggagctgact atcagtcagg tccagctttt
    16561 tcagaagtcg ttcaaatcga tggacgtctt cttacagggc aaaaccctca atctgctcat
    16621 caattgggcg aagtagtggt tgaagcatta aagcattaaa aattgtcagc ataaaaatgt
    16681 tttgcctatt ctaactatta cattacacac gctaaaacac gtcttttcgt tcttttctgt
    16741 ttcctatttg gacagaggta cgcaaaaggc gggttttttg cctttctaag agtacatttt
    16801 ttgaaacatc tcttcagaaa tgatactctt caattaggtc aactaattta ttactgagaa
    16861 gcagacctag tcacacggta ggatggacag agtgttttct gctacgctgc tatggatcat
    16921 aggaatgagt aaacatggtt taagaacatt gtgatttcag cccatgttgg cagtcgcaca
    16981 cttaataaga aagaggaata cagatgaaca ttgtaattgt cggcgggtcg tttgcaggga
    17041 ttcatgcggc tatttatctt cgccaattga taccaacaag tgaaatatat ttgattgaaa
    17101 agcaatcgaa gttagggtgg attcccagcg ggttcaatct gatagtaaaa ggaaaggtca
    17161 ccagtgaaaa acaactttct tggatcacca aagaagagct gatcgaccgc tatcgtatcc
    17221 atgtttacac agaaaagacg gtaataggat tggaagaaaa gaaagtcgtt ttagcaggcg
    17281 gagacaaact ggattttgat cgtttgattt tggcgactgg ttcaaatcaa aattttcgaa
    17341 atgttcctgc agaagcaagg ttcattcacc cagttaaaaa gatacaaaac atagctgctc
    17401 ttcagcaaaa gatcatggat gcaaaaaaaa ttgccatcat tggagcaggt caagcaggga
    17461 tcgaaatagc tgaaggctta gcatcaggaa aaacacaaat ccatctttat gaaagccgaa
    17521 agtcgattct ttttcgttac ctagaccctg aaatgacgga gccgttagta aaggaaatga
    17581 aaaatcaagg gatatcgctt ttcttagaag agcaagtcgt atctttgtcc gaaaatgaaa
    17641 ccgctgtagt agagacggaa agaagaaggg aaaactatga tcttgttttg ctggctaacc
    17701 attctcgccc caacaatcag atgtgggaag aacaattaac attgaatgat gatggaacga
    17761 tctgggtaga tgactatttg caaacgtctc aaaaagacgt ctatgcaatt ggcgatgcaa
    17821 tccaagtcac ttttcgccca acaaatgaaa aaatgtacgt ttctctagta aacaacgctg
    17881 tccgtacagc gcgaaatgtc agcaagacaa ttagtggttc tgcgacaaag gatcagggaa
    17941 cctatcgacc agtcggcaat cagtggtttg gctatttctt aggaagcgta ggtatgacag
    18001 aagaagaaag cattttttat ccccaaaaaa ttgaaacagg gtacgcggaa tacaagcttg
    18061 cagcaataaa ccagagcaaa gtcaaagtca aattcctatt tgattctgaa aaaaagattc
    18121 ttggagcaca gctccaaagc agaaaagaaa tgttccatct tcttgaccaa ctgacaatcg
    18181 cagtagaaga aggctggacg ttcgaacagt tagaagaaca tgagctgttt ttccagccag
    18241 aatatcgtgt acctaatatg gtgtggtcaa aggtggcgaa tactgcctat gaagattgaa
    18301 gcattattag aaagaaaaga gcagttacag atcctgattt tgcgaaacct tgttctgcaa
    18361 gggggaacag cctctatcaa tggattgaga gagcatgtcc aactgtcaaa aacctctttt
    18421 gatcaatatt tggaagatat tgaattgatt ggacggatga tgggaaaaaa agtagaagta
    18481 cagcgaaacg agtatcaggc gctgcttgtg ttggacgaag atgtcagttt ggaaaaaatt
    18541 cttttgtttt tattacagga aagcttgaag tttaaaatgc ttgtctattt attagaacac
    18601 cagcaagtat ccatcgttcg attagcgaca gcgtttaata tcagtgagtc ttctgttttt
    18661 cgaaagatca aagaattgaa ccagctctta gaggaatttg gattacaaat caaaaacgga
    18721 caattatacg gagaagagct acagatccga tatttttatt atgaattatt ccaatatatc
    18781 ccggaagatc aacgaccgct ttttttgcaa aatacaccag aaaaaagacc attcattcta
    18841 ggattgggcc gcgtattaga aacgacgttt acggcatcag cagaagcaca gattgcttgc
    18901 tggctcggga tcacgaaaaa aagactattg aatgaaaaaa gtacgtacga aaccttgaaa
    18961 gaaaagaaat tactctatca gtcggaccgg ctttatcaag caatcgatcc aatcatcgtg
    19021 atgcatctta gtcgaacagc agcagaactg aatgtatacg aaacaatgat gttttacagc
    19081 ttttttgttt ctttttcgat agtcgacgaa gaaatatttt atcaatatga tttgacacgg
    19141 agcaaaaagt tacccaccgc tgttttggat acttatattc gggaaacgat gctatggcat
    19201 tatcgtcctc gtcgtttgaa aatcaaagag gaaaaagcag taggctacca aatttcacaa
    19261 atcaacaatg aatggttttt ctttgttgga aagatcgaag tctacgagcg tgatcggctt
    19321 ttggagcagc agcaaaaaat gcttgggcat tccttaacac agttattgga gaaactacag
    19381 gaaacagcga tgcagcaatt gccaaggaaa agcaccgaag acagtgcact tagttatctg
    19441 atgatccagt atgccaatgt tcttttgatg atcgattttt atattgcgaa accagtagtg
    19501 atagggatcg atttagaaag tttgcctatt taccgtattg ctttccaaca gtatctgaac
    19561 agagaactga gaggagtcag cggtattgag attgaaagtt atgaagaagg aaaaaactat
    19621 gatttggtca taacgttttg tcagagaaac aagcaacaga atgaatatta tctatcagaa
    19681 tttgcgtcgc cttatgacat tatccgattg aaaagaagaa tagaaatgtt gaaaaaagaa
    19741 aagaattgat caaaaactag catcataata aaaaactgtc aaaaaaaaga aaaaaagaat
    19801 aaaattttga cagtttcttt ttttaaatga gtagtatgat aaaagcagtt aaagaaagcg
    19861 tttgcagata atggagggaa tcacaatgac agaaccaacg tatatcatgg cgattgacca
    19921 aggtacaact agctcacgag caattatctt tgacaagaaa ggaagacata tcggcagttc
    19981 tcaaaaagag ttcacgcaat atttcccgca agaaagctgg gttgaacatg atgcaaatga
    20041 aatctggaat tctgtacagt cagttattgc cggagctttc atcgaatcag ggatcaaacc
    20101 aaaccagatt gctgggattg gcataacgaa ccagcgtgaa acaacagtaa tctgggaaaa
    20161 agatacagga cgtccaatct atcatgcagt tgtttggcaa tccagacaat cagctggaat
    20221 tgcagataac ttaaaaaatg aaggatatca agaatttttc cacgaaaaaa caggactagt
    20281 gatcgatgcc tatttctcag caacgaagat tcgctggatt cttgatcatg tagaaggtgc
    20341 gcaagaacgc gctgaaaaag gcgagttaat gtttggaacg attgattcgt ggttagtatg
    20401 gaaactgacc gatggacaag cacatgttac tgattactcg aatgccagtc ggacgatgtt
    20461 gttcaatatc catcagctag attgggatca ggatattttg gatttattga atattcctcg
    20521 agtgatgtta ccaaaagtta cttctaactc agaagtttat ggttatacgc aaggttatca
    20581 tttttatgga agcgaagcac caatttcagg gatggcagga gaccagcaag ctgcattatt
    20641 cggacaaatg gcattcgaac caggtatggt caagaatact tatggtacag gttcatttat
    20701 tgtcatgaat accggtgaaa aaccgcaatt atccaaaaat aatttattga cgacaattgg
    20761 ttacggtata aatggaaagg tttactatgc tttagaagga agtatttttg tggctggttc
    20821 ttcgattcaa tggctgagag atggtcttca aatgttgcaa aaggctagtg attcggaagc
    20881 cgcggcaaaa cgctccacaa acgaagatga ggtctatgtt gttcctgcat ttgttggact
    20941 gggtgctcct tattgggatc aggcagcacg aggggctatg tttggtttga cacgtggaac
    21001 gacaaaagaa gatatcatta aagctacttt gcagtcgatt gcctatcaag tgagagatat
    21061 catcgatacg atgcaagatg acactggaat caagattcct gtattgaaag tggatggcgg
    21121 tgcagcaaat aatgaatatc tgatgcaatt ccaaacagat atcttgaatg taccgatcca
    21181 acgagcagaa aacttggaaa caacagcact aggggcagca tttttagcag gattagctgt
    21241 cggctattgg aaagatacag acgagattcg tgaattttat gaagctggca agctatttga
    21301 agttcaaatg gaggaagagc gccgagaaaa actttacaat ggctggaaaa aagcagtaaa
    21361 agctacccaa gcatttgaat aaggaggaaa tccatatgtt ttcaaacaag acaagacaag
    21421 atagcattca aaaaatgcag caagaagaat tggatctgtt gatcatcggt ggcggaatca
    21481 ctggtgccgg tgtggcagtc caggcagcag catcaggaat caaaacagga ttgatcgaaa
    21541 tgcaagattt tgcagaaggg acgtcctctc gctcgaccaa acttgtgcat ggcggtattc
    21601 gttatctgaa aacatttgat gtggaagtag tagctgacac agttggtgaa cgtgcagtcg
    21661 tacaaggtat tgccccacac atcccaaaac cagatccaat gcttttacca atctatgaag
    21721 atgaaggagc aacaaccttc aatatgttct ctgtcaaagt agcaatggac ctttacgaca
    21781 aactggcaaa tgtgacagga actaaatatg agaactatac cctgacacca gaagaagtat
    21841 tggaaagaga accatttttg aaaaaagaag ggctaaaagg tgcaggtgtt tatctggatt
    21901 tccgcaacaa tgatgcccgt ttagtgatcg ataatatcaa aaaggctgca gaagatgggg
    21961 cttatctagt aagtaaaatg aaagcggttg gctttttata tgagggcgat caaatcgttg
    22021 gtgtcaaagc tcgtgatctg ctgacagatg aagtgataga gatcaaagca aaattagtga
    22081 tcaatacgag tggtccttgg gtagataaag tacggaactt gaattttacg cgtccagtct
    22141 ctcctaaaat gcgtccaacc aaaggaatcc atttagtcgt agatgcgaaa aaactgcctg
    22201 taccgcaacc cacatacttc gatacaggaa aacaagatgg gcggatggtt tttgctatcc
    22261 caagagaaaa caaaacttac tttggtacga cagatacgga ttaccaagga gactttacgg
    22321 atccaaaagt cacacaagaa gacgtggatt atctattgga tgtaatcaac catcgctatc
    22381 cagaagcaaa tatcacattg gcagatatcg aagcaagctg ggcagggctt cgtccactat
    22441 tgattggtaa ttctggttct gattataatg gcggagataa cggatcgatt tcagacaaga
    22501 gcttcaataa agtggttgat acagtaagtg aatataagga aaataaagtt tctcgtgctg
    22561 aagtagaaga tgtgttgaac catttggaaa acagccgcga cgaaaaagca ccttctacga
    22621 tttccagagg tagttcttta gaaagagaac cagatggctt gttgacttta tcaggtggga
    22681 aaatcactga ttaccgtaag atggcagaag gagctttacg attgattcgt cagctgttaa
    22741 aagaagaata cggaatagag acgaaagaaa tcgactctaa aaaatatcag atttcaggtg
    22801 gaaacttcga tccaacgaaa ttagaagaaa cagtgaggga attagcaaaa gaaggagtag
    22861 cagccggttt agaggaagaa gatgctactt atatcgctga tttttacggg actaatgctc
    22921 gacgtatctt tgaattagca aaagaaatga caccttatcc tggtttgagt ctcgctgagt
    22981 cagctcggtt acgttatggc ttagaagaag aaatggtttt agccccaggt gattatctca
    23041 ttcgtcggac gaatcatctg ttgtttgaac gagaccagct ggatgagatc aagcaacctg
    23101 tgatcgatgc aattgctgag tattttgggt ggacagaaga agagaaggtg caacagacta
    23161 aacgtttaga agcattgatc gcagaatcag atctgcggga actaaagggg gagaaataaa
    23221 tgaacagtga tatgactcaa atcatggggg agtttattgg gacattgatc ctagttttgc
    23281 ttggagatgg tgtgtgtgcg gctgtgaatc taaacaaaag taaagcacaa gcttctggct
    23341 ggattgtcat tgcgtttggt tggggactcg ctgtaacaat ggctgtctat atttctggat
    23401 tcatgggccc agctcatttg aatcctgcag tctctctagc tatggcgatg acaggggcaa
    23461 ttagttggaa tcttgtcgtt cctttcatca ttgcgcaagt tttaggagct tttgcagggg
    23521 ccattcttgt ttggctttct tatttgccac attggaatgc aacgaaagat gaaagtgcga
    23581 ttttgggtac gtttgcaaca ggtcctgcta tacggaacta tccagcaaat gtaatcacag
    23641 aattaattgg tacttttgta ttagttcttg ggttgctagc ttttggacaa aacgaatttg
    23701 cacctggaac gaacgtattc gctgtaggtg gattgatctt agccatcggt ctttcattag
    23761 gaggaccaac aggatacgca atcaaccctg ctcgggactt tggtcctcga ttagcacatg
    23821 cggtattgcc aatagctaat aaaggaactt cagattgggc atattcatgg gtaccaatcg
    23881 caggtccaat gatcggagca attatcgcgg taggtgtcta ttcattaatg gtgtaaatct
    23941 ataaaacata ctaaggtttg acaatcaaaa ggcgtcttgc tatagttaag tggaaaataa
    24001 ataagaccta gaagaatggg gaatgttttg tgagtgtagc tggtatctaa tcagtaagtc
    24061 gaatagccaa taaacaaggt ttatttggtg tgcttatgaa acggtacctg tcaggaaaac
    24121 aatatctttg tattctaata aggataggac atgacttttt taagctgtgt tctatccttt
    24181 tatttatctc taggccctta tttattttgg gacaggtatc tcttcaagaa aaggagagag
    24241 tatgaacaac gaaatgatta gtcaattaca atttgatcag atcaaaaagg aaatccaagc
    24301 aagagcaatc ggaaactata gcaaaaaacg aatcagtaag atgacgacct cgtcaaacct
    24361 acaaaccgtc ttggatcgtc aagaagaaac aagagaagca cgcttgatct tagaaagtag
    24421 tcagcatgtt ccatttatgg gattgccgag gattgatgca ctgacagaac aagtcaaaaa
    24481 aggattggtt ctgtcgcccg ctgatttgat cgaatatgcg gattttctaa gaagcagtcg
    24541 gatgatcaca aagttttttg ataagaatca ataccaggca cctttgttat ttgcttatag
    24601 caagcatctt cctgatttga taaatgtaga agagttgatt gaccaaaaaa taaaaaataa
    24661 taaagtcagt gacgatgctt cacgtaattt gcgtaaagta cgcaaacaat tacaaataat
    24721 agaaaaagaa atccagtcaa aattgctgaa attcttgcgt catccgaaaa acaaagaaat
    24781 gatccaagaa gcaataatcg ttcaaaaagg tgaatgctat acgattccta tcaaagccag
    24841 ctataaaaat aaagtagatg ggacgatcat cgatgaatcg aacaaaggaa cgacggtttt
    24901 tattgaaccg actgtggttt ctaaactgaa tgaacactat cagttgttaa aagctgaaga
    24961 aatcagtgaa gaatatcaag ttctggctgc attgactggt gctattgcag aaaatgaaga
    25021 agcaatcgat ttattgatcg aaacgatgac ggtattagac atcatttttg ctcgggcaaa
    25081 attcagtcga gaaatcaatg ggatcacgcc taaaatcaat aaatcagaac atatcgtcat
    25141 caaacaaggc agacatccat ttttacctga tcatgcggta ccattggatg ttgaaatcgg
    25201 taaagactat cgaggcttga ttattaccgg agcaaatgcc ggcggcaaaa cagtcgtgtt
    25261 gaagacggtc ggtttattga ctttgatggc gatgttcggc atgcaagttc cggcaaaaga
    25321 agggacagag cttgctgtct ttgatgaggt tttcgtcgat gtcggtgacc accaaaattt
    25381 agaaaatgcg ttgagtacat tttctggtca tatgcaaaac atcgctgcta ttttgaaaaa
    25441 gataaaaagg aatacattgg tacttctgga tgagattggc agtggaacag agccaaatga
    25501 aggggctgca ttagcgatcg caatcatgga atctatgtat gagcaggggg cattgatcat
    25561 tgccacgacc cattatggag aaattaagaa atttgctagg gatcatgaag attttgtgcc
    25621 ggctgctatg gctttcgatc gggaagcact tcgtcctaaa tatcaattgc gtgttggaga
    25681 aacaggagaa agccaagctt tatggattgc tcataaaatg gctatgtcta tgaaattgat
    25741 ccagcaagca gagcgttacc tggaggaaaa agcctatcgg acacagaaaa aagaatttcc
    25801 tcctaagaca gcaggtataa ataggaagga caataaaaaa gaacgccagc tctttgcaaa
    25861 aggcgatcga atatttgtga atgaatacca aaaagaagca ttagtttatg aggatatagg
    25921 tgaagatacg attgccgtat atttagaaaa agaaatgatc cgtgtgcctc gtcagcgtgt
    25981 tcgtctggtg cgttcagcag aaaatctgta cccgacaggt tacgatttag acagtctgtt
    26041 tgttgatttt aaaacacgga aacagcagcg tgatctagag cgaggatcaa aaaaagccca
    26101 taaagcgtta gtaaaagaaa tgcgtaaacg acaggaagaa agaagagcaa aagatgaaaa
    26161 caacaagtga ttatttcaga ccaacgcata tgatcagtga ttgcaaaaaa ataacagcag
    26221 taaaatcatt ggttttattc tctatgtaag ctaaaataaa aaaaagagtg ctgaggaggt
    26281 gggacggtca aatgaaaata agcacaaact ggcgtaaaga aatccaaaca attccgaatc
    26341 ttttatcgat attccgtatt ctcttgctgc cgatttatct ttattttgtg ctgagacagt
    26401 cgttttatgt agcaggagtg gtcattgttg tctcaggttt gtctgattat ctagatgggg
    26461 tcattgctcg gagatataat caggtaacag acttagggaa ggtgcttgat ccgtttgcag
    26521 ataaattgac acaacttttt ctgattttgt ctatggcatg gtatcgaccg tggctttggc
    26581 tgttgtttgg tctattcttg ataaaagaag gctttatgtt tgtagctgga ttaatcgggc
    26641 tatctaaaaa tatcaaatta agtggtgcga agtggtatgg caaagtagcg actgctgtca
    26701 tctatgttgg catgatcctt ttgctgcttt tcccagaatt accaacgctg tgggtgagag
    26761 tcatttttgc cgtcatcact tacggtcttt tacaatcttt tgtcttgtat gctgtcgagt
    26821 accgaaagat gtttcaaaga aaataaagaa ggggtgtgac ctatgtcgca ctcctttttt
    26881 tgataaatag actcccaata gctacttctc gataataagc caaccaaacc aaaaacatga
    26941 gaagttgttt ttaaaattat ttcttgtaac tcggagttaa atgggtaggc tcacaacctc
    27001 tactgactaa ggcttgatat aagcataacc ttgggattgc agtttaacta aatcaagtac
    27061 acctgctggt acaacgatta cttgttcagg taaagaactt tctgaaatat gattagcacg
    27121 catagcattc gcacaagcgt gaaaaacgac ttctttcaaa tctaagaatt ctgcattggc
    27181 aaagtctaga taaccggtaa ttgcgatacc gttgacggaa acgacgatcg tcgtttcagg
    27241 agaagctttt atcaggtttt ttacgttttt ccctgtttct gaccattttt ctaattcatc
    27301 aatatggaaa acgactttca ttgaaatcat tcctttactt tttaataatc atcttgatcg
    27361 cttgctctaa tttagccgct tgttcttctg gattactatc gggattttca aagcaatttt
    27421 tcagattatc tgcaacgatc attcccatta cgcgatcaat gctggaacga acagcgctta
    27481 attgagtgat gacatccata cattcttttt catcttcgat cattttttgg ataccacgaa
    27541 tttgcccctc tgtacgtttc aaacggttca ggatattttt attctcaatc tccatagcac
    27601 attctcctct attttttata actcgataat acccctaatc ggtattttat gcaaatataa
    27661 aaagcagtta aaaaaatgaa gagtcattct ggttgacaga ataagaaagc tcctgtttaa
    27721 tataccccgt aaggtattta taaaatataa acgaggtgca gaaaatgagt ttgttatttc
    27781 agccaataaa atagataaca gtcccagaat taaaggaaaa gcttttagaa aagtcagtac
    27841 tacttgatgt tagaacacct gtagaatacc ggggcgggca tataaaagga gctaaaaata
    27901 ttccattaca aagcataaac agatatgatg gagacaaaga aaagaccgtt tatgtgattt
    27961 gtcaatcagg gatgaggagt aaacaagcag caaaagaatt aaagaaatct ggttatgatg
    28021 tggtgaatgt acgtggcggt atgaatcaat ggtttgatcg aacagtagga gggaaataag
    28081 atgaaaatcg ttattgtcgg aggtgtagca ggtggaatgt ctgctgctac acggcttcgc
    28141 cgattaatgg aagatgcaga aatcgttgtt tttgaaaaag gaccgtatgt ttcttttgca
    28201 aattgcggct tgccttatta tctttctggt gaaatcagtg accgggaaaa tcttcttgtc
    28261 caaacgccag aatcattatc cgctcgtttt tgtttagatg tgcgtccaaa tcatgaagtg
    28321 acagctatct ttcctgaaaa caaaacggta gaagtcgtac atgagggtca aaaacacttg
    28381 gaacagtacg atgtattggt tttatctcct ggtgcaaaac cagttgttcc gtcgattcca
    28441 gggataacag aagccgacaa tgttttttct attagaaatg taccagatat cgataaagtg
    28501 atgcatgcat tagaaaaaca gccaaagcgt gccgtgatcg ttggtgcagg attcatcgga
    28561 ttggaaatgg cagaaaacct aaaaaaaaga ggtttagaag tcatggtgat cgaacaagca
    28621 ccgcatattc ttccgacact ggatgaagaa atggcagctt ttatagaaaa agaattgtct
    28681 catcaaggag tagaagtgat tacttctcat gctgtcgctg gatttgaaga ccatgggaaa
    28741 cgattgcgac tggatgacgg gcgtaccatt cctgctgatt tagttatttt atccattggt
    28801 gttcgtcctg ataaccagct agcagtgact gctggaatcg aattaggtat acgcgggggt
    28861 atcctagtag acgaacgtta tcaaacgaat attcctgata tttatgcggt gggggatgct
    28921 atcgttgtaa aacagccaat cactggaaaa gatgcactta tttctcttgc ttcaccagcc
    28981 aatcgtcaag gtagacaagt tgcggacacg atttccggaa tttctcgaag aaatcaaggc
    29041 agtattggca cagcaattat acgaacgttt ggaatgactg ccgcatccac cggattaagt
    29101 gaaagaacag ccaaagaaaa cgagctgtct tttgaagtcg ttcatgtgtc aggaaaagat
    29161 catgcaagct attatccaga agcaacagat attttactga agttgatctt ccatccagag
    29221 actggcgaga tttatggtgc acaaggtgtt ggggcaaaag gtgtggataa acggatcgat
    29281 attttagcaa cagcaatcaa agggcatttg acgatcttcg atttgccgga gttagaattg
    29341 acgtatgcac cgccatttgg ctcagccaaa gatccagtaa acatgctagg atatgcagca
    29401 atgaacattg cagaagggct tagtgaaacc gtacaatggc aggaattgcc gacagaatta
    29461 gccaaaggaa aaattttatt agatgtgcga acagcagaag aattggaaaa aggcaaattc
    29521 aaggaagcca aacatatccc tttgaatgaa cttcgggacc gattaaatga attagacagc
    29581 caacaagaat atatcgtcag ctgtcatagc ggattacgta gctatctagc ggaaagaatc
    29641 ttgaagcagt ctggctacca cgtaaaaaac cttgatggtg cattttcttt atatcaaact
    29701 gtccgacctg aagaactgat atatcctaac aaatgatact cgttaaaaag cttgcattcg
    29761 actcttaaaa gaggtaaact aatcggttcc gccactttaa aaaaagagga gaatgtgtat
    29821 ggtcaacaca caaaagcagt tagaagaagc atatctgaag aacgtgtatc aaaagctaat
    29881 cgataaaaga aacgaactgc gtgaatcgat ggatacggca aaagtagaag gaaaccagtc
    29941 gctgaaagaa atggctggcg agcttcgatt gaattttgac agttacttag ataacctgga
    30001 tactttttcg atgattgaaa tgaaaaatag agaaatcgat cagatgaata tcaaacagaa
    30061 aaacgcagaa aatcagctag caaagatcga acgtttattg aaaagtccct actttggaaa
    30121 agtcgttgta gactttctgg ataatgagcc aaaagaatcc ttttatatcg gtacagcaaa
    30181 tttcacagat gatgcagatg aaaatcttgt ttatgattgg cgttcaccaa ttgccgaact
    30241 tttttacaat aacatgattg gcccatcttc ttatacggtt cgtcaaaata aaatcgatac
    30301 ggttatcaaa gaaagacgtc aatttatttt ggaaagagat cgtttggtca agttttttga
    30361 tacgtctgta gctatccaag acgatgtgtt attagaagtg ttaggtcaag atgagaccaa
    30421 tgaaatgaat gctatcactg ctacgatcca atcggaacaa aacacaatca ttcgggatgt
    30481 gaaaagtcaa aatatcttgg taaatggcgt agcaggtagt ggtaaaacct ctgctatcat
    30541 gcagcgtatt gcgtatctgc tctatttata tcgagatacg atgacatctg atgacttgct
    30601 gattctatcg cctaatcatc ggttcatcga ctatatttcg aatgtcctac catcattagg
    30661 cgaaagaaat ccactgaatc taaccatctt gcagctagtt tcacagcttt cagcagagga
    30721 aatagaaggt gaagaagctt atttcaaacg tatcaccaaa gaaaatgtat cagagcaagc
    30781 agaacacttg cgtagtaaag agtatattga tgatctaaaa cgatcagatc ctttgtttct
    30841 agatcatcct gattttatcc gtggacttac taaaaatgga aaaacagttc tttctaaaaa
    30901 aacgatcaaa aaaatatatg agaaagtccc tgctcatcct aaattgatcg acaggcttca
    30961 agcaacgaaa aaggcactga tgagcgaatg gaagaatcat cttttgaaac aagcaaaaag
    31021 tcctgctgtg cagaatcagg tactctcgtt gacagaagat agacagttga aattattcgg
    31081 tgaattgatt tctgatgatt cggaacaaag tatcgcagct tatgcaagga aacttttaca
    31141 aaaaaactat cgaaaaataa ccagacaaat cgaagaaatg gcatgggtgg atgagcaccg
    31201 attgtttgaa agaatttatg aaaaacgata tggctcagct tatgtttggc cacacacacg
    31261 tactgttgat gaagcagtca ttttattagc tattcatcat ttgcttgttg aaaaggtaaa
    31321 tgtaccagcg tttcgctatc tattgattga tgaagtacaa gattataccc tggcacaatt
    31381 aggtctattg atcgaattat ttccaaaaac tcactttact ttagtaggtg acgagaatca
    31441 agcaatcttt aattcttcca ctacttttgc tgatattatg cgttgtttcg acgactacca
    31501 tctgccgatc catcggtatg atcttagaaa tagttaccgt tctagcggag caatcacaga
    31561 gcttttcaaa acctatgctg tggatcaaga gaagatcgac atcgtatcga tccgaccgca
    31621 aggaaaagaa cccgaatacc gctcttttcg ctcctctgaa gaattgttgg aaatactagc
    31681 tgagattttg gcttctctaa aaggagaaaa agcggcaatt atcacccaaa cagaagagga
    31741 aacccaaacg ctacaagaat cgattaaaaa aagttcatat ggattagaaa actgccagat
    31801 catccctttg agtctagcaa aaggactgga atttgatcat gtaatcttgt atccatttga
    31861 aaacgataga gatgagcaaa gaagacgcag acaaatgtac acggcgatat ctagaggaat
    31921 gaaaagcatt gtcgtattgg aaagagaaac ataattaaga gataaaaaga taaaaagaat
    31981 aactgataaa aagaacaaaa tatttttcat ttgccgatga gaaatgtttt gttctttttc
    32041 ttaggaaaaa tttaggaaca ctttatgtga aaagaaatga aaagtatggt aaactcctac
    32101 gtcgtaacaa aaatgtgagg tgaggatatg aaaaaagtaa caagagaaga agttgcatcg
    32161 attttggtga aagataagta tttttcaaaa ggtaattatt ggttaaaaat ttggcaaacg
    32221 ctagtggctc ttttgggttg gatgatcgtt gttcttcctt ttatatgggt atttttccca
    32281 ttaacaagac cagaaagagc caaagatttt tacttgtacg cttttttatt ggaaaagaaa
    32341 atgctgtatt tcttcatcgg cttttttgca ttgatctttt tcagcttcct gattatctca
    32401 tttgtgttga ctagatggaa caatcgaaat ttgtatagaa aagtaaataa gtcttttgaa
    32461 tatgaagatg aagaattgaa aaaaagacaa gaattacttg aagaaatgta tacggaacgt
    32521 tttggaacaa aagaagagag agaaacggtc cgtttttatt ctgtctccga agagaaaaat
    32581 ctagatacga cactcattgc agatttgtat aaagaaaatg gggtggaact gaaatgacct
    32641 caatcactca tacaggtaat caattcctag atacttgttt gtctatcatg tatttttttc
    32701 tcgtttctta cccgatttta ggcggatttg catggtttat tggtgtctgg tgttatgttt
    32761 ttttatacaa acataaacaa aaagaatggg tagatgttcc atcgggtgtc gaaccgttca
    32821 ttacgatcat ggtgccagcc cataatgaag agatcgtgat cgaagatacg attgagtatt
    32881 tgatgacgaa attgaattat cacaactatg aagtattagt gaccgatgat ggttcaacgg
    32941 accaaacacc agaaatatta gccagactga tgaaaaaata cgctaatctt cgtgtggttc
    33001 ggatcgaaaa aaataaaggg aaagcccatg cgtttaatat cggacttgcc tttgcaaaag
    33061 ggaaattgat tttaagcaat gatgcagata cggtaccgga accagatgca ctgaacaggt
    33121 atgtgaatta tttcattcgc cctggtgcaa gacatattgc tgcggtcact gcaaatatgg
    33181 atgtccaaaa tcgtaccaag ttgattgcaa aatcgcaaac ggttgaattc tcgagtatcg
    33241 ttggtatcat caaacgaaca cagtcagctg ttttcggtgg cctttatgcg tacagtgggg
    33301 caaatacgat gtattgaaaa gaagccttga tcgatgtggg cggattcagg caagatcgag
    33361 cgacagaaga catcagtatt gcgtgggatc atcagttgaa tgattgggta tctgtttttg
    33421 ctcctgggat catctttttc atggaagtac ctgtgacgtt gaagatgcta tatcgacaaa
    33481 gaaaacgttg ggcaaaaggt ggaacagaag tctggttgac gaatttcaaa aaagtgatgc
    33541 tccatccttt tgaacatatc ggaagaacga tcatcttcat tgatcaaaca ttgagtattg
    33601 tctggtcaat attcttttgt atttccgtga ttctttttat tggattgatt gggcattatg
    33661 tgtaccaagg aaactatgaa cagatctatg tcacttttac attcagtttt gtgttcatct
    33721 gctttgaaat ggtcgcagga tttttccagt tgttagcttc gctgattgta gatgatcgac
    33781 gaagaaaatt gaaatatctt cttttcgcgc cactatatat gcttttgttc tggatagtga
    33841 acgcgataac gatcgtgacg actttcattc cagcagtcaa aacgatttta ggttatggaa
    33901 gcgggacatg gaaaagtccg gaacgtacaa aaaaataaaa gatgcaccgt tttttagaca
    33961 aacagcctgt tgtgtttatg gttcttccaa tcatttttgc ttataataag aaattagtta
    34021 aaaagtagta aggatgggaa aagtgatgaa aaaaagaatg aagccgttga tctggccgat
    34081 tgctgtttcc ggtattttct tcttcggaca tacggctttt gcagaaactt ctgaacctgc
    34141 tcaaacagag ccttcaacaa ctataagctc ttctgatgct gaaaacggaa cctcggaaac
    34201 agaaatgact tccaatgtac cgacagataa ctcaggagaa acgatttcgt tttcagaaga
    34261 tacatcaacg aatagttcta cctcttctga ccaaatcgat catttgcaag acttggaaaa
    34321 taaaagaaaa gaactcgaag aagcattaga agctgcaaaa aacaaattga aagaagacca
    34381 agcagcaatc gatcaagatt tagctgatct taaaaataaa gcaggaaatg accgtgcaca
    34441 acttgagaaa aagcttgctg aagcgaaagc aaaagcggaa gctgtccaaa cagatcttga
    34501 cgggaagatc caacaagcaa aagagttcaa tcaacaatta gatcaggttg ctgaaacagc
    34561 ttctggaaag ctaagtcaaa ctcaagcgga tatcacagcg gccagtcaag cgatccatgg
    34621 aaagatcagc gaagcgcaaa cagttgctga tgattttgaa gcaaaaaaag cagacttagc
    34681 agctagtttc caacgaataa aagatatttt gcattcggca ggagaaaaat cagtggcctc
    34741 tggaaccacg acagaaacag ttcagcaaac gacagagcca tccgaatcaa caagtcagac
    34801 tttaccaaaa acaaatgaaa aaacagcttc tgtcaattat tccttcgtag gcacaggtgt
    34861 gctagttgca gcagcttacg gaatcattcg caaaaagaaa taaaaaagag agaatgggaa
    34921 ggtagtgaac ctcgaagtta gactgaaaaa tctaactctt ggatcccact acatgtccca
    34981 tctcttttta tttattaaga cagctattcc tacttatctt gtggagacag ctgttccact
    35041 acatgtgacc atcagcatgc cattgtcgct gcctaatact tcgtagtcca tttttacgcc
    35101 gatgattgca tcagctccta atgcttttgc acgttcggcc atttccgcaa gggcttcttc
    35161 tcttgcgccc attagttcat tttcataact ttttgaacgg ccgccaaaaa cgtttcgcaa
    35221 accagccccc aaatctttca atacattgat tccagtaata acttcaccaa aaacgattcc
    35281 tttataagct actacggttt ttccttccac actattcgtg gttgttgtaa tcatttgatc
    35341 actcctctct ttaatgaaag tataacaaag ttcaagatgc gcaacacata ttttcccaaa
    35401 attaggaatt tattatatac ttgaattgtt cattaatgaa agcgaaacca atcgttagca
    35461 actatgtaaa agaaaggaag attcttgtga aaaagagtat tttgcttatt tctcatagtc
    35521 aaaaagtaac agatggtatt aaagaaatga ttgagcagat gcaaaaatcc gaagaggttc
    35581 agatcttttc tctcggcgga ataaatggcg agatcggatc agacccgatg aaaatcgtag
    35641 aagctgtcaa tgagtcaagc gatgcagcta gttattttgt ttttgcggat atcggcagcg
    35701 cggtattgaa ttcggaattg gcaaaagaca tgttggaaga agaacagcag caacgttatc
    35761 atctggtgga tgcgccttta gtggaaggag cttttgcagc agccatcact gccggtataa
    35821 cggatgatac tcagcaaatc ttaacagaag cacaaaatgc cggaaagaaa ggatgggaat
    35881 aaattgaaaa aaatcatgaa tgatccaagc aatattgtcg aagaaatgct ggaaggatta
    35941 gtaaaaagct atccagaact tgttcatcgt gtagaatctt cccgcgttgt agccaagaat
    36001 cagaaagcag aacaagttgg tttggtttct ggtggaggaa gcggacatga accatcccat
    36061 gccgggtttg taggggaagg aatgttaagt gcagcggtgc ttggtgatgt gtttacttct
    36121 ccaactccag atcagattca aacggctatc aaggaagcgg atagtggaaa aggagtttta
    36181 ctgattgtta agaattatac aggggatgca ttgaacttcg atatggcaaa ggaactagct
    36241 gctatggacg atattgaagt agaaagtgtg atcgtagatg atgatatcgc cgttgaaaat
    36301 agtacgtata ctgctggaaa acgcggagtt gcgggtactg tccttgtaca caaaatcgtg
    36361 ggcgatgcag caagaaacgg tgcttcccta gcagagttga aagaattggg agaaaaggtc
    36421 gtacaggcaa ctaaaacgat cggtgtagcg cttcgagcag caactgtacc agaagtaggg
    36481 aaaccaggat tcgaactagg agaagatgag atcgagtatg gtgttggtat ccacggtgag
    36541 ccaggctatc gtcgagaaaa gatgcagcct tctaaagtac tggcaaagga acttgttact
    36601 aagattttag atgattactc gaatttgcca aaagaagcag gtgtattcgt caacggaatg
    36661 ggcgggacgc ctttgatgga acagttcgta ttcatgaatg atgttttagc tctgttggaa
    36721 gaaagaggcg tcaatgtagt tttccgaaaa gtcggtaatt ttatgacctc actagatatg
    36781 caaggccttt ctctgacgct gatcgacttg acggagacac aatggaaaga ttctttagaa
    36841 agcaatgtac agacgatttc atggtaagac aatctcaaga taaaagggtg ataatatgaa
    36901 actaaccgtt caagaaatcc aagaatggct ctcacaattt gcagaggcaa tcaatcagaa
    36961 caaacaatat ctcagtgatc tagatacccc gattggcgat ggagaccacg gaaataatat
    37021 gggaagaggg gtttcagctt atgaagaagc ttttcaaacc gatcatcctg aaacaattag
    37081 tgatacattc aaagtattct ctatggcgat gatatcgaaa gttggtggtg cttctggtcc
    37141 gttatacgga tctgcattca tgaacatgat gaaagcgaca aaagaggtcg attctattga
    37201 ttctcaagaa aaattaggag aagtgatcga gcagggaaca gcgggtattc aaagccgagg
    37261 aaaggcagaa gctgacgata aaacgatgtt agatgtttgg ctcccagtta cagaggcatt
    37321 gaaagcagga aatctaacaa aagaaatcat tgaacaggca aaagaacaca ccaaagactt
    37381 ggtagctaaa aaaggacgag cttcctatct aggagagcgt gcaatcggac atatcgaccc
    37441 gggagctgct tccagtgcca ttttatttac gacactactc aatgtgatca aaacttcatg
    37501 accgttcctg taaatggaaa cagaggctga actcgaaaag caagcagttg ctttttcgat
    37561 tcagcttttt tacagttgtt tcttttaaat aagatagtta aaataaaaaa gaaaagaggg
    37621 aacaaaatgg atctaatgaa agaagactat cagttatttg acagagacaa ttatgcattc
    37681 aaacaactca aagaaataca tacaccagat gaagtcgaac aaatcaaaca agaatacaaa
    37741 gcacattggc aaaaatggaa agagatccaa ctccagactg ctgcactttt gcctgatacg
    37801 tatggcatga gcaaacctaa aatcgagagt tggacgaatg gctggaattt acgcagtcat
    37861 ttttggagcg cgtaccgaag cgaatctcgt caagacgaga atgcctgtct agcagttttg
    37921 ttgaatcaaa aacaatacca aatctatttg atgtatcagc actataaaag tgatacgaga
    37981 gaaggaagcg ttgaaggata caatcaatcg ctatctttac tgcaggaatg gagtacccag
    38041 gtagcgattg aagaatatta tatctggcct cagcctgaaa atgagctgga agatcatttg
    38101 cctttatccg tttatttatc agataagagc aagcaagaag agctgcgaga gacaatgggc
    38161 aatcgaacct tccaacttgg caaactgttt ttttctccaa atgaatacac aaacattgaa
    38221 gagaaaactg ctgaggcgct caaagaacta gcgccattgt atcatgcgat aaaaaataag
    38281 ttatgaaaaa gctgattgcg ttgcgtacta aatagatggc acatcatcga atccactgct
    38341 ctcatgatgc aacataaaca gacacctgcg aaaaaatcat ttttttcgca ggtgtctgtt
    38401 tggtttttcc atcagccaaa tcagaataaa aatttaaaca aatctaaaaa cataatggtt
    38461 aacgatctct aaaaaacaac gattacttgc gccctctcat tcttttatat atatgtttcg
    38521 atttacataa cagaaaaaaa gaaaaaatat tcatttcgtt atcaaacaag gaaaagtaag
    38581 aaaaaggctt cttttttcac tgataagcct atggtaaaat ccatttaaag cgttttcttt
    38641 gtgaagggaa gcaagaagga gcaagcatga aaaaaatcat tttcagagga gtcatagtca
    38701 tcattgcact gagtatagga gggaaattat tgatggatag aagagaaaaa gacaatgaag
    38761 aactgcgtac catccaaacc gatctggcaa attatttgta taatcattat gagatttcaa
    38821 cagtggatga aaagcgagaa aaggaaatct tcaaagaatt taatcaaggt aaaggagaca
    38881 tgactgaaca ggaatttttt gaaagattag attctataac tgagtatatg gatattgaga
    38941 aaatagaatt tacaggtttt tctgtaggtc cgatgaaagg gttggtagtt ggatttatca
    39001 tcaatgatgt ttacccagat gaaacgacgc tagatacgag atcagccgaa acaaataagt
    39061 ggctgtattc cttcaacact gggaatacta ggaattcata tgtactaact aaaaaaaatt
    39121 cttcgacaga tgaaaaaatg cctgaagaaa acattatcta ttatgacaag ggagtcaagt
    39181 gatggaccaa aacaaagaat tctctaaagc agtatatggc gttgaaacag ggaaagtaaa
    39241 agtcaacgaa aaaatcgttg aggatgaata tcttgttatt gatgtcattg ataccgacaa
    39301 acatacaatc ggaaaagaaa aaacaccaaa gcaaaacggt atgcaagcca tggtcgtggc
    39361 tgaaatcaaa gatgaatata aaagtaagaa gcaaaatcaa ttacagcaaa tttccgacta
    39421 ccccaaatcc gtcatcaaaa aagacctgac catcgcctac gcgggcacta aaaactggca
    39481 ggactggaaa acgaacatcc gagaagtcgg cttcgaggac aaacacgaca acggcgcctt
    39541 ccagtctgct ttagcgtatg ccgatgcaat cgaaaagacc tattctgtca aagacggcta
    39601 cactatttcc acgaccggtc attctttagg tggagcgcag gcaatctatg tcgctgtttt
    39661 aaaaggttat cacggattta catatgcagc ggcaggccca ggcttgagtg agaagcagct
    39721 catgaattat gaagggcaga tcatcaatct gtatgacact tcagacatcg tgactagtgg
    39781 ctggctgact ggcgggaaag gccaactgcc atttcacagt ttcggaatcg acaacgccgg
    39841 ctggaaaaac tatggacacg atctaaatca gttcaatctc gacaaaaacg ggaattacat
    39901 cgataaatat ggcgatatcg tcatctattc cgaccaacat ggaggggtcg ccctcgaaca
    39961 gaccttactc gcccagcaaa tcatcaaaaa caaagctatg atccgtcaaa tggagacata
    40021 tggcgaaaaa aatcaatggg agaaagatcg gattagtcaa ctgaaagaag aaaacaagtg
    40081 gctccagcta caaatcagtg ctttctccaa gctagtgact tggcggaaaa gattcacagc
    40141 gagcagcggc ggtctgtcga ccaatgaaca gatctattta gacgaccagc aggcgctgat
    40201 gatcgtgaaa catgcggctt ccgtcttcaa tcaagcgatg gaacaagtgc tagtcattta
    40261 ccagcgtgga attcgtgatt tggaacagct gtgggcagat ggtcttgcga tggtccgcag
    40321 acagacaccg cttttgtctc agaacgaaat gctcgatgct ctgcgagaag tcggttgtac
    40381 aaagcagacg atcgttgatg aacctacgca ggaattccgg gaaaaaatct tcaaaatcaa
    40441 gcagctttca gcagaattct ctaccttggc aaaagagatc gaagcgaaaa tcaatgagtt
    40501 ggtccaacgt gatcgggact tggcacgaca attattttaa aacaaggagt agtgagatgg
    40561 aaaaagaaaa ccagattcat gaaacatacc gaaaagaacg actgcagcta gaagatcagg
    40621 aagatcagct cagacagatg caaaagaaca tgcagcagct ggcagaaacg acttattcca
    40681 atatacgttt ttcggtgcgt tcctttgaat gcccgaagga ttctttgtat tttgcacaaa
    40741 aagaactcag acgtttagaa gagaggttct ctcatgaatt gatgcagaaa cgaaaaaaaa
    40801 tctatgatca gcaagatgaa gtcgaacgcc gttatcgtgc tgatctgcaa cgattaaaca
    40861 aaaagtaaca ggaaaggaga taataagtga tcgttctaga ggaaaaaatt tgggaagact
    40921 accagaaaga gatacaaaaa atctcagcaa gagctttagg gattgcgatg actcaagaca
    40981 aagtcacgaa atgcaaaaag gcgaaacaag ccgcagaggt ggaagcaagc acggcaaaaa
    41041 aagcggtatc ctctgcaacg actggattgg aaaaagcagt aaagggtcgt ttcggcaaaa
    41101 aagtaacagc ggcatttagt aaaaaaagcc gaatgttagt aagtttttag aaaatcagct
    41161 acagtaataa agaggttgtg aaaaagctat tctgcatcaa gcagtaagaa caagcagaga
    41221 aaaatagctt ttcatatttt tcatctgatt gggcttagtg tcgtagatgc aagcttttga
    41281 acaccgttta aggaagaggt tgtgaaatca gtgttttgac ctgagtatta gagaaaaaaa
    41341 cggaaaaata gctcctcata ttttgccgtt ttttgagcta atgccgaggg tcagctgatt
    41401 gaacaccgtt tattcaaaaa ataggagttg tgacaaggcg tttgtcacag ctcctatttg
    41461 acataagcgg caattttcaa ttttacttat agatagtatc aaaaaatgat tttaggacat
    41521 aaaacactca tcaccattat taccgagagt ctgatcctgg tggccgaggt ggcaagcggt
    41581 atgcttttcc atgaaatgag tattctgatc gtggtcataa acggcatgtg tctgcttact
    41641 tatcgcacaa aaagaaaaat cttgatacaa atcaattaac caagttaaaa gaacaagtag
    41701 aataacaaac aaggaagaaa tccaaaggag gaatctgcta tgagacatga aggattgtgt
    41761 gtttcactcg ttccattatt caatcatctc gagatagaag atcagcaaaa aatccactcg
    41821 ctagtgacac acagaaaagt ggaaaaagga gaacagattc ttgcgccgaa tggagagtct
    41881 caattagtga tcgtggcttc cggcagtatg aaagtgtatc atttatctgc tagcggaaag
    41941 gaacagcttt tacgagtagt agagccgggc ggatatgagg gagagagcca gttgctaggc
    42001 gttccaaatg agtctttatt tggcgaagca ctagaaaaaa cggaaatttg tgtattgaga
    42061 caaaaagatt tctcggaact tttactgcat tatcccgccc ttagtctgaa acttttagca
    42121 atcaacgctg agaaattaac aaaagtacag cagcagacaa cttttttgat gatggaaaaa
    42181 gtagaagaac gagtcgctgt ctatctgctt gatttagcta aagcgacagg gaagaatcac
    42241 tttttcctgc caatgaaaat gaaagaactt gctgcattta tcggtacgac ccccgaaact
    42301 ttatctagaa aatttaaatt attagaggag aatggttatg taaaaagaga gaatcgggaa
    42361 gtctggatca ttgataaaga gcagttagaa gacttgtaaa tacaaaaatg acctccaaat
    42421 gttggatttt tgatcccaat ttggaggcta gtatgaaatt gaacatgctc ttttttgtta
    42481 tccttccact aaatctcttc tcctataacc gatagcacca aggacgatca gtgcaaggct
    42541 gatacaggat aacgtaagga atacggaaag atcgaaagaa tcgtttggca tccgtggtac
    42601 ccagctaggg accgctgttt tggagaacca ttctggcaaa tccaaaatcc cgccaaagta
    42661 attgagcgca aaggagtaag ctaagtagat ataagcaaat ttacccattc gaggaagcca
    42721 tccgaataga aagccactca tcccgataaa cactaaaaca gcaggcagaa agttgaatcc
    42781 ggctttaaga aacagcaata gatccatatc tttcgcatcc ttcatcgatg ccaaagcaga
    42841 aagtcctagc gcaccagaac taatcagaat cgacaatata gaaaacagga tcgctagtaa
    42901 cagatttgtc caataaagtt ggttcctaga aatttttgtt gcgaacagct gattcatacg
    42961 tccgtgggtt tcttctgtaa ataaacgatt gatgctagca attggtaaaa tagcagctag
    43021 acaaatcaag acgaccatga tcgtgcttgt aaagtttttt tcaattgaga cgccagattg
    43081 gataaacatc tgtttcatta agtcgttact ttctaaaaac tgttgcatat tcccgtagat
    43141 agagccgtat gccgccccca tcaaaccaaa agtgatgagc caactgattg tcattccttt
    43201 atttagatag aataataaac cacgcaagga tagaagagaa cgtttagcat acggttttcc
    43261 ttcacgttcg gggatatagc cggtgttcaa gtctcgatgt tcctctaaaa caaatgaact
    43321 gattatcaag atgagactga acaaaaacaa ataaagcaaa ggaatccagt tattagttgt
    43381 aaatggatag gtcaaataag accagcctaa aggattccac atcgaaaggg aggaaacagc
    43441 tacatctgtt attgctctta gaagatagag tccacctaaa aacgctagag acagaccatt
    43501 cgaactggct gaacttggca tcagttgtgc agcaaacagt gcaacggcac tccccaaaat
    43561 ccccgtacta cctacagata gcccaaaaag ccaagcacct tctgcagtga tcgaatctgc
    43621 ttgaaaaaaa atcatcgtac agctgatcga tattgctaaa agcaaattga tcagaatagt
    43681 ttcagcgatg acagctaaag agtttgcttg acgtccgact cgaaaggaac gaataagttc
    43741 aatcagacca agctcttctt tttttctcgt ttggctcact acatgcaaaa ccgagatcac
    43801 agcagcaaac aaactgcaaa aaagcaacat ttcatttgca tacatcgctc ctaaagtata
    43861 ctcagcagca ttcgaagcgg gagttggtcc gaccattgct atcattgccg gattttgcat
    43921 tgtttcgtat aatccagcta acccttgtcc tgaagtcaat tcttcaaatg cagggacaaa
    43981 accaccagaa aaagcagtga caccgactag ccatatcaag atctttttcc agtcacggct
    44041 aaggttttga gaaaaaaggg tggaccagcg tgaaaattta tcagtcattt cgattcctcc
    44101 tcatccttca tagtgcctca tgaataaatc ttcaagagtc ggtggaatag attcaaattt
    44161 tttcacacct aatttggaag cttcagctaa aatcacatcg aaagcttcgt tgtcagcagc
    44221 aaattgagca aggtggcctt tttgcacaaa atcatggaca ccgttcaatt gtgctaagtg
    44281 gctgttatct gcttctgttt ctatagtaat ggtcgagcgg gtcaaatgac gcaattcatc
    44341 gagtgttcct tcttctacga ctgttccctc gcggatgatc acgactttat ctgccaaccg
    44401 ttcgacttca ctgagaatat gagaagaaag caggatggat ttaccctgtt ctttcagttt
    44461 ttcaacttct tcttgaaaaa tggtttccat caagggatct aaaccagatg taggctcatc
    44521 aaataaatag agatccgaat caacagataa agcagcaatc aagcccactt tttgacgatt
    44581 tccttttgaa tagttttttg cttttttctt aggatcaaga gagaaacgtt gaatcagttc
    44641 gtcctttttt tgagcgttcc cttttccatg aagcttcatg aagagatcga tgatcgctcc
    44701 tccagtcaaa tttccccata aagagatgtc acctggaaca taagaaagac gttcatggat
    44761 agataaacta tcttcccata catcttgacc aaaaatcgtt gccgttccgc cgtctttttt
    44821 tataatccct aaaagtgtcc gaatcgtcgt ggattttcca gaaccatttg gtccgataaa
    44881 tccaacgact tcacctgaat gtacggtgaa agagacgtct tttaaagctt cgaaacgacc
    44941 aaagttcttt ttcaaatgtg tgactttgac gatttctgcc ataaaaaccc cactcctttt
    45001 ttaaaaatat atactaaata agattaaatg gtttaataaa atggttttat acctttttat
    45061 aaaacttttt ttattatttt agtacataat attcttttta taaatttctg tcaatgaatt
    45121 cataaacaag gaattgctgg cttgatactt tctcaataga tagttataat aaacctcaga
    45181 ataaatttga actatatata ttataaaagt ttaaaattac gggaggctct accaatgaac
    45241 ggttttgaaa agagagcaca agagaaaaag aaagagattt tacaagcagc ttttgacttg
    45301 atgaacacag acataggaac cgctggagtg acgatggata aggtagctga gagcgctcag
    45361 gtaagtaaag cgacgatttt taaatatttt ggcagcaagg aagggctgat ttatgaagta
    45421 tttttcacat ttatgaatga tttagggcaa gaagcgaaag cgttgattgc ggaaaatctt
    45481 ccatttgaag aaacgttgat tgccatgagt gaaaataaaa tccgctatct caatcgagtg
    45541 aacaaacaat tttatatcga tttaatgaca tgctatacaa aaaaagaaaa aaaagaattt
    45601 tctgacgcga tggatgcgta tacgcaagaa agtttcagta tcatgcttga tcttttccat
    45661 cgagggcgta aagagggaaa agtggatttg aaatattctg atgagttctt gatcctctat
    45721 tttcaagcaa tggtagaagg aatttctaag ccagagatat acgcaaaact gcttccatat
    45781 acagcagaat ggacagaagt cctgatcaag ggaatcgcgc cgaagaagaa ataaagcata
    45841 aaaaaagccg aaactgacga taaaatgtca gttcggcttt tttggtttat aaatgacgta
    45901 aaatcaagcg gattctcgtg cttctttgtc atagacatca tgaacgttga tgaatggatc
    45961 aagaataggg ttttgtccgt ctaaatctga ttggaaccga atgccatcga tattgattcg
    46021 agcatttcga tagaaatccg cacctaattg tccccaagcg ggagtagaag catctacatt
    46081 gcagaaaata tcgaaacctt gactctttaa gtaagcaaat ttttgatttt ctgctgaata
    46141 aggctgccag tcgctaatat ctgcaccaaa tggataaatc aggatatttg tttttcctag
    46201 gataggtgca acttcttgct gccataattc attgtctttt ttgacatcct ctaatgaagc
    46261 ttgagtcata ttcaagtgtc cccaggtatg ggaagcaaag ctccagccat ctttcttcaa
    46321 ctgatcagct acttttttag cttcttcgat ctcttttttt gttttctcgt tatctccgta
    46381 ttgagacttg gatgttcgat aacctaaaac accgttgtaa cctgtcaggg ccaaggtgcc
    46441 ttttgcgcct cgataagaaa aatcaggatg tttcttgaca aaagcatcga tcagaggaac
    46501 catgtcaaag tccccaattt cttccttatc attctcttta taaacatttt tgatttcatt
    46561 gttatttgat acgactaatc ggctaggaaa accggaatta tccatatatt cataatagct
    46621 gacatcatct tgcgaaagaa ctaatggttt ttttccttca ggtagcgcaa gcccgttaaa
    46681 ggaaaggttt ccttgttcgt ctttttggat caaatcattc aaattgacta aaacaaaatc
    46741 attttgatag agttgttcga tcgaacggtt gaactcttca acagttacca tatagtcttt
    46801 atatccttgc gactgttgag agtggaaggc tttttgcggg tccacgatca agctgtgaaa
    46861 aaagacgtga gagatctgag taggatcaga ccagtcaatc agttgttctt tctccttttt
    46921 cagtgtttga agaagctgct gtgtttccgc agatccgttt ttactgtttt tctccaataa
    46981 agcaatcgct tggtcataat catagctttg tgccagattt tttgcttgag taactagctg
    47041 ttgcttatca tccattggct cttttgtctt ttctgttatt tttttttcag gaatgatcgg
    47101 ttcttttttt gtgtatgctc ggatgacaaa aaacagactt aaaattaaaa taacgacagt
    47161 taaagcagaa gtagctccta aaataatttt ttgctttttt tccatatacc tcgtcccttg
    47221 ctttttcatt tttttactcg tcccattgct cattgtaacg aagaaagaat aagaggaaaa
    47281 gtaaagtgac ttctcgataa attgcagaaa aaatacagaa aaaatagttt cttttaacaa
    47341 tattaaaaat aaaatgttcg ggattagagc ggattcaaaa aagaaaaaat gaggaattta
    47401 aaaaaaatat cctttttctg ttgacaaaga aaaagttgcg ctctatgata gacacatatt
    47461 aataataaca agacgaagat cgaaacgaag tagttgttaa agccaatgtt tcagaaagct
    47521 ggtggttgct gcaaaccagt acatgcttta aagcgaatta catttcagga gtctctggca
    47581 gtgattgcca cgcgacagcg ttaaatgttc gagaggtatg ttggatatct agacatacaa
    47641 attgggtggt accgcgagat ttcgtccctt ggctatatgc caagggactt tttttatttt
    47701 ccatcccgaa gaaatactta atgacgcatg ctagcaggtt cctgctttca taaaaaacta
    47761 cacggaggtc agtaaaatga atattattga tgaattaact tggcgcgacg ccattaatca
    47821 gcaaacaaac gaagaaagac ttcgcgagct cgttgaagaa aagagcattt cactttattg
    47881 cggcgttgat ccaactggtg attcgatgca tatcggacat ttgatcccat ttatgatgat
    47941 gaaaagattc caattggcag gccatcatcc atatattctg attggcggcg gaacaggaac
    48001 gatcggcgac ccaagcggcc gtaaaacaga acgtgtgttg caaacgatgg aacaagtgca
    48061 acataacgtg gatgcacttt ccaatcaaat gagaaaattg tttggtaaag acgcaaatat
    48121 cacatttgtg aacaactacg actggttgtc aaaaatctct ttacttgaat ttttgagaga
    48181 ctacggtaaa aacttcaaca tcaacacgat gttagcaaaa gatatcgttg ccagccgttt
    48241 ggaagtaggg atctcattca cagaattcac ttaccaaatc ctgcaatcca tcgacttttt
    48301 acatctgcat aaaacatacg atgtacaact acaaatcggt ggagcagacc aatggggaaa
    48361 tatcactgct ggtttagacc tgatccgaaa attagaagga ccagaagcag aagcgttcgg
    48421 tttaacgatt ccattgatgt tgaaagcgga cggaacgaaa ttcggaaaaa ccgcaggcgg
    48481 tgccgtttgg cttgatccta agaaaacttc accatttgaa ttctaccaat tctggttgaa
    48541 tcaagatgat cgtgatgtag tgaaatactt gaaattcttt acgttcttat ctcaagaaga
    48601 aattgaagaa ttagcgaaaa aagtagagac agaacctgag aaacgtgaag cacaacgccg
    48661 tttggcagaa gaagttacaa gatttgtgca tagtgaagaa gacttgaaag aagcgcaaaa
    48721 aataacacaa gcattgttct ctggaaatat taaagaacta aatgcggaag aaatcgcgca
    48781 aggattcgtt aaaatgccga acgttgaaat ttcaagcaca cctgaaaata tcgtggagct
    48841 acttgtttcc acaaaaattg aaccatctaa acgtcaggca cgggaagatg tttcaaacgg
    48901 agctataagt attaacggtg accgcgttac cgatttaaat tttgtcataa atccatccga
    48961 tgaattcgat ggtaagtttg tggttattcg aaaaggtaag aaaaattact ttttggccaa
    49021 agtaattgat tagaatttag taagtttttg ccttgaaaaa ggggaatact attacaacat
    49081 tccaattttg aaacccatcc taaaatagaa cgctggttgt cgttaataca atctttgcga
    49141 gggagtttac ttttgtaaat tccctcgttt tttttaatta tttcgaaagt atgaacaatt
    49201 cgaatgattg atacatatta aaatacgatt aaaaaacggt cgaaagctta gtttacagaa
    49261 taggagaaca ggtgtaatgt agggctcgac aaaatttaaa tattttttta ggagggtttc
    49321 actatgagtg aatcattgtc gaaagatcta aacctaaatg ccctattcat tggggacaaa
    49381 gctgagaacg gccaaatcta taaagctttg ttgaatgaat tggtggacga acatttaggc
    49441 tggcgtcaaa actacatgcc tcaagacatg cctatcatta cgccagaaga aaaaagcagt
    49501 gctagttttg aacacactgt gaataaaaca aaagatgttc tatcagaaat ttcagcgcgg
    49561 atgcgtaccc attccgttcc ttggcataat gccggacgct actggggtca tatgaactct
    49621 gaaacattga tgccatcttt actagcgtat aattttgcta tgctatggaa tggtaataac
    49681 gttgcttacg aatcttctcc tgcaacaagc caaatggaag aagaagtagg catggaattt
    49741 gcaaaattaa tgagctataa agatggctgg ggacacatcg ttgctgatgg ttctttagca
    49801 aacttagaag gactttggta tgcccgcaac atcaaatcat tgccacttgc tatgaaagaa
    49861 gtgacacctg aactagttgc tggaaaaagc gattgggaat tgatgaactt gtcaacagaa
    49921 gaaatcatga acttgttaga cagtgttcca gaaaaaatcg acgaaatcaa agctcactca
    49981 gcacgtagcg gaaaacattt agaaaaatta gggaaatggt tggttccaca aacaaaacac
    50041 tattcatggc taaaagctgc cgacatcatc ggtattggtt tagaccaagt aattccagtg
    50101 ccagttgacc ataactaccg catggacatc aacgaacttg aaaaaatcgt tcgtggtcta
    50161 gcagctgaaa aaacaccaat cttaggtgta gtcggtgttg taggatcaac agaagaagga
    50221 gcaatcgacg gtatcgataa aatcgttgca ttgcgtcgcg tattagaaaa agacggtatt
    50281 tacttctact tacacgtaga tgctgcttat ggcggatacg gccgtgcgat tttcttagac
    50341 gaagacaaca acttcattcc atttgaagat ttaaaagatg ttcattacaa atacaatgtc
    50401 tttacagaaa acaaagatta tatcttagaa gaagtacaca gtgcatataa agcaatcgaa
    50461 gaagcagaat ctgtaacgat tgacccacat aaaatgggct atgttccata ttctgccggt
    50521 ggtatcgtca tcaaagatat ccgcatgaga gacgttatct cttactttgc gacatatgta
    50581 tttgaaaaag gcgcagatat cccagcatta cttggggctt atatcttgga aggttcaaaa
    50641 gcaggggcaa cagcagccag cgtttgggca gcacaccatg tcttaccttt gaatgtcaca
    50701 ggatatggta aattaatggg tgcatcaatc gaaggtgcac atcgtttcta caacttcttg
    50761 aatgacttgt catttaaagt aggagacaaa gaaatcgaag ttcatccatt aacttatcca
    50821 gatttcaata tggtagacta tgtattcaaa gaaaaaggca acgatgactt agttgcaatg
    50881 aataaattga accatgacgt atatgattat tcttcatacg tgaaaggaag catttacggt
    50941 aatgagttct taacttcaca tactgacttt gctatcccag actatggcaa cagcccatta
    51001 caatttgtga accaactagg attctcagat gaagaatgga accgtgccgg aaaagtaact
    51061 gtattgcgtg ccagcgtaat gacaccatac atgaacaaag aagaacactt tgaagaatat
    51121 gctgaaaaaa tcaaagcagc tcttcaagaa aaattggaaa aaatctacgc agaccaatta
    51181 ttggcaagcg aagcaaaata agcaaggtaa tcatgttaga aaacagcttg aatactaact
    51241 gtttctgacg catgtttgga tctgaagagc aaggggagac ctaagctctt cgatcccttt
    51301 gttttgaggt tgtctttgaa gaaacccttc aagcaataga ctcaaagaat cccctcaaat
    51361 attcgctgaa ttgtttcgtt tattgctgaa actacttcaa agacaccgtt attttatcca
    51421 aaaaatcaag gagagtgtca acggtgagta aatctgaagt aagtaaaatg ggccttggga
    51481 cctttatcgg tctgacaatg gcactgtgtg caacggttcg gagtattcct acgttagccg
    51541 cagtcggttg gacattgatt ttttattcaa tttttgcagt tttgtttttt gccggtccga
    51601 tatcaatggt ttccggtgag ctttccacga tgcttcctca agaaggcggt cctcagttat
    51661 gggtaaaaac agcactgggc agcaaatggg gatttgtcgt tgcatggcta ttatgggtgc
    51721 aaatgttccc aggtatggta atggtcgctt ctactttagg accattgtta ggaaacacat
    51781 ttggtaatgt agaacttggc aataatcact ggttcgtttt aggttgtatt ttagttatct
    51841 actggatcat cacgatcttg aacttgaaat tcgatatggc aaaagtcggc gggaacatcg
    51901 gtgtatggct aggggtatat atcccagtag tcatcatgtt cgttttaggt gttttggcag
    51961 ccttcaaagt cggactagtc tcaaacggct atctaggtga cttttcatgg tcaaaagctt
    52021 tccctgactt ggaacatatt gattcattga aatacttagc tggtatcaca tttatttttg
    52081 ttgggatcga aatgagttct gtttacatgc ctcgtttgaa agacgcaaca aaaaattata
    52141 caaaaggcgt atttatcgct ttgatcggat tagttttatt gaatgtcatc aatgcgatgc
    52201 ttgtagcaaa tgtcgtacca gatggaaaaa tggaattagc aaatatcacg cagcctatct
    52261 tgattgactg tcaaatccta ggcttaccag aagtgatcgg aaatatcttc agcttcatgg
    52321 tatttattgg tgtgctttta caattatcag cttgggttac tggaccatca aaaacaatca
    52381 tccaagtagc tcgtgaagga ttccttccac caaaattcgg attccataaa gaaaacaaat
    52441 acggcgtatc tagaaatgtc gttctaacac aatcaatcgt tatttcatta tttgctttgc
    52501 tttatggtgt aatggatgac gtaagtgctg tattcttgac acttacaaat gctacaactg
    52561 ttatttattg tatcgtgtac attttgattg ctgtttcatt gcttaaaatg cgtaagaaac
    52621 atccagaatt cgaacgtcct taccgaattg gtaaaaacgg aaacggatta gcttgggtag
    52681 tcagctgcat gctgatcttc tctatcatcg ttgtcgtatt tgctacatta ggtacagcga
    52741 cattgagtga tgcattatta gtagcagcta tcacagtggt catgttcgtt attccattga
    52801 tcatcaatca cttcaagaaa gattcttggg ggattgaggt agaaaaaagt ttagaagaaa
    52861 aataaaaaaa ataagtcttt cagccgacgt tttgtcgctg atttagtagg aggtcctgcc
    52921 aagtgaatag ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat
    52981 aaaaatattt ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga
    53041 gtactgatca tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt
    53101 ttgttgtttt atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc
    53161 aaaggaatca ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt
    53221 gcttcatgga ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt
    53281 tctgtgcgtt tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc
    53341 ggcagttcat ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta
    53401 cttggattca atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat
    53461 aactgctcgc ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta
    53521 ttcgaacata tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt
    53581 ttttatacga tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg
    53641 atccaaaaca tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt
    53701 ttattttggg gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca
    53761 gctgctctcg ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac
    53821 atcttgatga atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct
    53881 cgaggcggga tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta
    53941 ggtgggttac tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag
    54001 atggaccgac cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg
    54061 atcgttggag aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt
    54121 gatgacgttg gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc
    54181 gtcaattctc tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt
    54241 agcgcgttgc attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata
    54301 ttaggcggtt ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc
    54361 tgggacagaa gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttttaaa
    54421 tttttgtgaa tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg
    54481 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcttttt tacagataaa
    54541 caatgtgtta gaagtagtat cttcttttac ataaaaagct gaccatagga taaatggtca
    54601 gcgtatttag tgagtcagct atttagtatg ggttttctaa aggaacgact gtctctcttt
    54661 tgatcaagcg atgagggatg accaaacgta cgccactgct caattcttca tggatcaatt
    54721 cgcgcagctt ttgcatagcg acacgtccta attcggagat atcgatatcg atacttgtaa
    54781 ggtaagggtg tgttaatgtt gagaagatcg agttgttgaa gctaatgacg gagagtgtct
    54841 ctggaatgac atagccgtac atttgcgcca actgcatcgt gcgaagggcg aagatatcgt
    54901 caatgaccac caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag gacagataat
    54961 cctccggatg ctctagtact acactggata acgcaggaag accagccatc atcattgctt
    55021 tttgataacc gaaatatcgt tcgaaataca aactttcatg agtcgtgttt gtcacaaaca
    55081 ggatattctt atgtccattc tggatcaaaa attctgttgc ttgtttacct agaagctggt
    55141 tatcattatc tacataaacg atttcgttct catgatgata aggctgtccg atcaaagtga
    55201 aagggatgtg gttttggtat aaataatcaa tgacaggatc ttgactatct gaatacacta
    55261 gaataaaacc atctacttgt ttttgtaaat gcatacgctg gacgttctct aacagcaatt
    55321 caaaattttt agcagtagcg attgctgtag tcatatcata attccgcgct tcttcgttga
    55381 ttgcttccat gatctcaaga taaaatgggt taccgatccg ctcttttgaa tcaagagggg
    55441 gtaagattac gccgatggca ctggaaatgc gttttcctag attgcgggca gtcatgttag
    55501 gaacataacc tagttcatcc ataatttttc ggactttctt ctttgttgtc tcggaaatac
    55561 taggatgatc gttgatgaca cgagagacgg tcgaggtggc aacaccggct tttttcgcca
    55621 catcttttac ggtaatagcc atgataaaaa cacctttctc ttgttattgt gcagagaacc
    55681 gataaatcgt ctgactgatc cgttcttgac caggatggag gttgatcgat ccaaatttgg
    55741 ggaaatgaac gatatcaggg atttcttgcg gttcgattgc taatccgtag tttgaatgca
    55801 tctgttttcc attgacagag aaatcagctt cgaatccagt cgtagaaaat aataccatac
    55861 ttttattaga agttgctata gtcatttggc gaccagaatg cttctcgaat aattgtaagg
    55921 aagttttgga aagcctagga tgatgcaaaa gaaatacatc atctagtcca tctggataac
    55981 atgcaaggat ctcttgcagt gttggggatt ttctaaagtc gattggcagt ttttcttttt
    56041 ttgtccaatt tccagtaggc agtttttctt gatctaattc aagcacacca tctaaaaaga
    56101 ccgttaactg atgttcatag atgtctcttg ttccatttcc tgaaaggttg aagtaggcat
    56161 ggttggtcgg attgcagatg gtttcaacaa ggctactagc agaggttgtc atctctaatt
    56221 cgttttttgt taaacggtaa gtgatcgtag cagtgattgg tcctggatag gtagaaaatt
    56281 catcttttaa gtaaaagacc acacctattg atgatggagt tttgaaaact tctacttccc
    56341 aaaattggaa agaccagcct ttcgtgccac cgtggatatg gtgtgatccg cagtttttct
    56401 ccaatctatg ctcaccccaa gaagcatccc ggatccttcc ggctacaggt ccgacagttg
    56461 ctccaaaaaa gcttttgtct gttagaacat cttcgaaacg atcgtaagac aataaaacat
    56521 tttctgattt tccttctttg tctggtgcga agatttggtg caggcgggca ccgtaattga
    56581 gaagaacgac ttctaagtag tcgttcttca atgtgatttt ctttatttca gtctctggaa
    56641 ctggtacgac ggagatctct ggattatcca tggtcatgcc acttttcttt caagtaatcg
    56701 atcgtaagaa aagtcgtatc agggacaagc gggatctcct ctccaaggtc ttcagcacgt
    56761 cccacgccaa ttggttgagc gccacttgag aggatggctt ggataccggc ttgcgcgtct
    56821 tcgatcccga tacattcttc tgcttttagt ccgacagctt ttgctgcaag gaggaagata
    56881 tctggcgcag gttttccgtt tgttgcttct gccgggtcgg caatagcatc aaagtaaggt
    56941 gtaagaccca ttttttcaag taggaatggc ccgtttttac ttgcagaagc aagagcaatc
    57001 ttgatttttt cttcttttaa agaatccagt aaaggcacga ccccggggaa gacatctttt
    57061 ggttcgatcg tctggatcat ctctaaataa taatcgtttt ttcttttcgc taattctgca
    57121 aattcttcct ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa agagagtgag
    57181 tcttctcggc tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt aatgccgatt
    57241 tcctgaccaa gctttcgcca agcacgataa tggaattcag cagtatcggt aatgacaccg
    57301 tctaaatcaa acaatacccc tttaaacatt tgcttcctcc tttcttagcg ttatttctag
    57361 acgatccgct aaagaataag actgttcata gacgtaaatt tctagaggaa aaccttctaa
    57421 taactcgaaa gtaacttgtt catctgtgac tgagatgaac aataaacgtc cgcgataatt
    57481 gatatggaaa gaataagctt tccaagtttt tggcagaaat ggtgcgaatc tcaactgatc
    57541 atgggcagtt ttcatttgag caaatccttg gacaatcgac aaccagctac cagtcatgga
    57601 agtgatgtgc aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca agcgagctgt
    57661 tcgctggtac atttctacag ctttgctttc cattcctaat tctgctgcta aaatcgaatg
    57721 gatacttggc gaaagggaag actcatggac agtcattggt tcatagaaat caaagttttt
    57781 ttgcttttct tcttttgtaa aagtatcatt gaagaaataa atcccctgta gtacatctgc
    57841 ctgcttgatg aagcaagatc taaggatttt gtcccaagac caatgctgat tcagaggaag
    57901 atcgcttgtg ggaatatcgg atacaggcat gagatcttta tctaagaatg tgtcatgctg
    57961 gacaaagatt cctaattctt cgtcaactgg gaagtacatg ttctctgcga tatctttcca
    58021 tttggctagt tcttcttcgc tgatagtgat agcagcttct ttttgataag ttaaataatt
    58081 ttctcgcgtg taacgcaaga cccaagctgc gatcgtattt gtataccaat tgttattgat
    58141 attgttctca tattcgtttg ggccggtcac accgtggatc atgtatttat ttgctcgttt
    58201 tgaaaaatgg acacggtctg cccagaagcg tgcgatttct gctaaaactt ccagtccttc
    58261 atttttcaaa tattccgtat cgccggtgta attcgtataa ttgtagattg cataggcaat
    58321 cgcgccattt cggtgaatct cctcgaatgt gatttcccac tcgttatggc attcgacacc
    58381 agtgaaggtg accataggat aaagcgcgcc ttttaatcct tgttgctgag cgttgtggat
    58441 tgcttgcggc agttgattat gccgatattt cagcagattc ttagttactt caggtttggc
    58501 taaagcaaga taaagaggga cagcatacgc ttctgtatcc cagtaagtgg ctccgccgta
    58561 tttttctcct gtgaatcctt tggggccgat attcaatcgt tcatcttcgc cgtagtaagt
    58621 agagaatagt tggaacaaat tgaatcgaat accttgttgt gcttcttcgt ctccttcgat
    58681 gaccacatcg gcaagcttcc atcgttctgc ccaagccgct gtttgctctt cttttgcttg
    58741 gatataattg tttgtctggt tcgttaattc tgagacagct ttttctgctt gctgcgcttc
    58801 tggaatatct ctactagtaa gaacgataat ttctttttcc actgaccatg tctcaccttg
    58861 agataattct gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat agtcagggat
    58921 gactgatttt ccattaacga aatggcgcat tgctgcagta acagtaaatt gttcaatccc
    58981 aaagttgttt ggaatagttt tagtcgtcaa atagccgatt tcttctatag ttccacgatc
    59041 aatctcattc caaaaatgtt catcatagtt gctgtcttcg ttttgaacat ttccatccaa
    59101 ttttgatacg agtttgactg ttccgctacc ttcaagcatt tctatagtta aatagatata
    59161 agctgattct tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta ctttattagt
    59221 agaagtagtg attgtgaatg tacgagataa tacaccattt ttcatatcta attcccagta
    59281 aaagtcttct ggttccaaag aagccaagtc gatcagctga tcgttgatcg aaatatctat
    59341 cgcgataaag ttcaaggcgt tgatcacttt tccaaagtat tctggataac catttttcca
    59401 ccagccgaca cgtgtcttat cgggatacca gacacccgcc aaataagtac cttgatggtg
    59461 gtcccctgag taagtttctt caaagttccc gcgcatcccc atataaccgt ttccaatact
    59521 tgttaaagat tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc tgattttcca
    59581 tggatcaata gcaaatagtc gtttgatctg tttcattttt ctcctcctaa tgagctgttt
    59641 tatacgttca taataaacgc aaccgattgc acaagtcaat agacaaaaga aaatatttca
    59701 tagaaaaaag cttaaaaaaa taaattttta ggagaaaatt atagaaggga acacacatat
    59761 tataggtttt atagaatggt atagacagaa aaataaaaaa taaagcattt acaaaaaaga
    59821 aaacgcacgc tataatacgt ttaacgaaac cgattgcgta aaggagctaa acggaatgaa
    59881 aaagatattt agttttgagt tttggcaaaa atttggaaag gctttaatgg ttgtggtagc
    59941 agttatgccg gcagctgggt tgatgatcag tatagggaaa tcaattccct tgatcaatcc
    60001 tgattggacg ccgcttgtca caacgggcgg agtaattgaa aatattggtt gggcaattat
    60061 tggtaatctt catattttat ttgctttagc aataggagga agttgggcaa aagaaagagc
    60121 aggcggagca tttgcagctg ggatctcatt tattttaatc aatcggatca ctggggctat
    60181 ctttggtgtc acaagtgaca tgcttgcgaa tgaagatgca tttacccaca ctctgttcgg
    60241 tacgaagatc atggttaaag gattttttac aagtgtattg gaagcaccag ctttgaatat
    60301 gggagtattc gtagggatca tcgctggatt tgtcggggca atggcttata ataagtacta
    60361 taattataga aaattgccgg atgctttgtc atttttcaat ggaaagcgct ttgttccgtt
    60421 tgttgttatc ttatggtcaa caatcgtggc attgattctt gctgtggtgt ggccaaacgt
    60481 acaggctgga atcaataatt ttggtttatg gattgcagaa tcacaagaaa gcgcaccgat
    60541 tctagcaccg tttttatttg gaacattaga acgtttacta ttgccatttg gattgcatca
    60601 tatgctgacg attcctatca actatacaca gcttggcgga tcgtatgaga ttttatcagg
    60661 cgcacaggcg gggacacaag tatttgggca agatccgtta tggttggctt gggccactga
    60721 tttagtgaat cttaaaggtg caggggatat gtcgcaatat gagtttgtgt tgcataactg
    60781 gacgccggct cgtttcaaag taggacaaat gattggttct tcaggtatct tgatgggaat
    60841 ggcttttgcg atgtatcgta atgtcgatcc tgataagaaa gcaagatata aatcaatgta
    60901 tttttcagca gcgttggctg tctttctaac aggtgtaaca gaaccattgg agttcatgtt
    60961 tatgtttgct gctgtgccat tatatgtcat ttattcagtg atccaagggg ctgcatttgc
    61021 gatggcagat atcttgccat tgcgggtcca ttcatttggg aatatcgaat tgctgactcg
    61081 gacaccgtta gctatcaaag cgggtctagg tggagatctt atcaacttcg tgttgtgtgt
    61141 gatcgctttt ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa aattcaactt
    61201 tgctacaccg ggacgtaacg gaaattacga caatgacagt gaagaaaccg ttgtatctaa
    61261 cagcggaaca ggaacagcag atcagcaagt ggtccagatc attcatttgc tgggaggaaa
    61321 agaaaatatc aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg taaatgatcg
    61381 agaaaaagta gggactgaag aagcctggaa acgagcaggg gcgatgggat tgatcgtcaa
    61441 agacaatgga gtccaagcag tttatggacc aaaagcagat gtcttgaaat cagatatcga
    61501 agatctgctg caatcaggag cagagattcc agagccagaa atggtttcta caggaaatgg
    61561 acaagcaggt agtaagcaat acttaggtat agaacaagaa ctagtttccg cagcttctgg
    61621 agaagtgatt cctttatctg aagtaaaaga tcgggtattc tcacaaaaaa tgatgggtga
    61681 cggctttgct gttattccaa catcaagaga agttgttgcg ccgatcgctg gaaaagtgac
    61741 aagtattttc cctagcaaac atgcaattgg gctggaaacg aaagatggta tcgaagtttt
    61801 gatccatatg ggaatcgata cagtccaaat gaatcagcca gtttttgaga ttgctgtcaa
    61861 agaaggccaa gaagtaggaa caggaacgaa attagctgag atggatcttg atgtgatcca
    61921 aaacgaagaa aaagatccaa cgatcatgat cgtatttaca aacgataaag tagaagaagt
    61981 agtgatcaag caattaggta cagtgacagc tggtaaagtg atcggctcca ttaaaatgta
    62041 aggagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa acaaaaatag
    62101 ctcctcctat ttttcgtttg gttgggctta ttaccgagaa gcagcagttt gaatactgtt
    62161 tagtggagag gtcgtgaaaa agctgccctg caccaagtga taaaaacagc caaacaaaaa
    62221 atagctcctg gtccattgag cttattactg aggagttagc aggagaacac tatttgctgg
    62281 agaggttgtg acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga caaggagaaa
    62341 accaatgaaa ttcatgacac tcaacacgca tagctggctt gagccagaac cagagaaaaa
    62401 attgcaagaa ttagcagata agatcctgtt agaagattat gaagtgattg ctttacaaga
    62461 aatcaaccaa ttactggaat ctgaagaagt agaacctgcg aagctaatga agttctgtca
    62521 ggtaaagaat caagtaccta tccgtaaaga taattttgcc taccgtttag ttcagctttt
    62581 aaaggaacac ggaaaagagt atttttggag ttgggaaatg agccacatcg gttatgataa
    62641 atatgaagaa ggaaatgcac tgcttacgaa aaaaacattg gaaagtcaag tccttaccgt
    62701 ctcccaatct caaaagaaag aagattatca gacacgcaag atcttgattg ggaaaacaaa
    62761 gatagatgat caggacattt ttgtctcgag ctgccacttt tcttggtgga cagataaaaa
    62821 aagtggcttt tattttgagt ggaaaaactt ggaaaactac tttttggaaa ctcgcgtacc
    62881 actttttttc ctaggtgatt tcaataatcc agtagactct caaggttatt atactgtaag
    62941 agaaagttgc cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag gaaaagcagc
    63001 tactgtggaa aagaaaatag atggttggga acaaaataca gaaaaattgc gtatagattt
    63061 tattttcgtc cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg atgggatcga
    63121 tagtccaatc atcagtgatc actacggggt ggaaatcgaa ttagatgtga acgaataaaa
    63181 aatataacat ataacataaa aaaactgatt aaaataaaac tctccaaacg attataccgt
    63241 ttggagagtt ttttgatcgg atgtatttat cccttttgcc taagcacgta aaagcttgta
    63301 tctatgataa ataaataaac atagcaaggt aaaccagcca atcaaagtaa gagaaagaat
    63361 gaaatagaaa tagattggcg tttgataatg aagcgtgagc tcgttgattc cttttttctg
    63421 cttcacgatt ggggtcccga tatctgttac ttcataatca gtcaatgttt ttccattttg
    63481 ctgtagcaca gtccgatcgt agacgataac agggatatga acagttttgt ccccagtgtt
    63541 tttccacttg attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg tttcgttttc
    63601 tagtatttct tcgttatacc ggtcgtattt attgtctttt gtctccttat agattggcag
    63661 atagtcaggt gtggtttttt ggaaaacgaa caagctggta gacatatctt tgctgaagaa
    63721 tgttttcctt gcttcttccg gcgtatcgaa tagataagta tgtcgactga caaatgtttc
    63781 attttcccac ttttccagat gctggtataa gttctggatc gtctgtgctg aaaaaataag
    63841 aatgccaaat atacttaaaa gacggaacca cttttgttca gaccactggt tcagtgaaag
    63901 ggcaaggtaa agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg gaaactggat
    63961 caaactaata aaaggcacat ttttttcgat caatgtcgtc catggaatca agctagtaga
    64021 aagaaaaaag aaaacaagac ttgtgtacaa cacgattttt tggaataagg tgttcttttg
    64081 ttgaaagaaa ataaaaagca aaaaacctga taatatcaat ataggaaagg ccttagggta
    64141 atggagccaa taactgccat ccagatctat cgccttttga ctcatttctc tgttaacaaa
    64201 gggaggaaga atcttattcc cactgtaaac aatagctaat cctgcccaaa tatttgctgt
    64261 cagaacaaaa taaagcaaga ccgacttaat cagttgaagc agtgcttttc ctttttcagt
    64321 ctggttaaaa aagtaataaa gataaaaagg gatatacatc aaagctaaca tgatcgaaga
    64381 cagtaaatgg atctgtgtca taagtcccac tgagacagcg actttgataa cagggaactt
    64441 tttatgaagg acgaaatcag caatagggat catacataaa ggataaaaac aagtccccca
    64501 gctagtgaat ccttggttga tagaccagta ttgaatagaa tacgtactca tgaataaaac
    64561 ggctactggt acacttaccc aatttcgaac attgacatat ctcagtaaaa tcagcataga
    64621 aaatcctgaa ataagataaa gaatgaaatt tgaaagaatc tgataatgaa accagctacc
    64681 tgcaagcaac acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa cggcattcac
    64741 gatcctgcca ctttgctgga atccataaag ggaaggaaaa taatggaaat ccccatattt
    64801 aatttgttgc gcggcatcat agaaccgatt gtaatggaat ggggcatcag cacccaaaat
    64861 tagtttatgt gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa aaattgctaa
    64921 aaatgggcca tattttttca attgtcgaac tcctttgcaa actttagata aattcatcat
    64981 atatcctatg cgagaggtag gcaagaaatt agaaaattca tgaaaatatt tatgaatttt
    65041 acaagaaaaa cataagtgaa agttctaagg agagaggatg cgacataagt tttgttgcat
    65101 ctccttgttt tgaaacagaa tttttcttaa tactcgaaac aaagtagctt tttcatgacc
    65161 tctcggtaaa cggtgttcaa tcagctgctt ctcggtaata agctcactag cctagaaaat
    65221 atgagaagct gttttctgac tagcgctctt attactcaaa gcataacgca gttttcacaa
    65281 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca acccaacaaa
    65341 aaatatgagt agctattttt tggggctgta cttattactt gatgcaggac agctttttca
    65401 caacctcatc ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac
    65461 aaaaaaactt gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac
    65521 ttttgtttcg tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc
    65581 cattttatgt ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa
    65641 aacggaccgc agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat
    65701 taactgacca aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa
    65761 gtacaacatc cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc
    65821 aattaaatat ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg
    65881 tcttgcaagg aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc
    65941 acagcggttt acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca
    66001 cgtttatttt gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg
    66061 tactgccaga tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc
    66121 ttacatgtac gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc
    66181 cttataacga atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc
    66241 aatggtttat tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac
    66301 gactaatcta tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg
    66361 attcgtcagg gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac
    66421 ttactagaaa tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg
    66481 atcaattgcc tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt
    66541 cttttggtat tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg
    66601 taaaagagct tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa
    66661 aaatgatccg cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt
    66721 ggctgcatgc taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag
    66781 tagaatctgt taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag
    66841 aaattattgg ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg
    66901 aataccggca acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa
    66961 cactgacttt gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag
    67021 gttttacaat tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga
    67081 aatggaatca aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa
    67141 ttttttgctg ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag
    67201 ttgttacaat ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    67261 cggcaaaata tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg
    67321 atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta
    67381 gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc
    67441 tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa
    67501 aaacggcaaa acatgaggaa ctatttttcc atttgtttct ctaatactca ggtcaaaacg
    67561 ctgatttcac aacctcttga taaacggtgt tcaatcagct gaccctcggt attagctcaa
    67621 aaaacggcaa aacatgagga actatttttc cgtttctttc tctaatactc aggtcataac
    67681 gctgatttca caacctctct ttcacaacct tattttactc gtttataatt ttcatcgaag
    67741 tattttccgc tacggatatc atctggcatt ccatcgtatg gttcttcgct aataacatct
    67801 tcattgtcta tacctgcatc acccatataa gtgatcgtag caaattcagg aacaagtaat
    67861 tttggataag tttcatattt ttcacgagct gcttccattt ctgcgatatg ttcgttttgg
    67921 aaagtaacag tgatttctgg atgttcttca acccatttag ggaaaaagat cattccgtga
    67981 agtttctttt catttggcat aaagtccaat gccacgtctg tccctgttgg ctctgtccaa
    68041 gtgattttga atacgccgtc cgtcaattta acgatatcag cttcttggtc ttttacccaa
    68101 cggcctttga ccattccgcc atggatacgg taatcaacag tatggtcatt tttggcatac
    68161 cattcatatt cccaaccatt gtcatacgta tagatagagt gagttccaat aaaatcttcc
    68221 aattctttaa attctttcat gtttaagcat ctccttataa attaaaacat gtaataaact
    68281 acatgttttc gattactcat ttatgataga atggatttaa agatttgtca aggagggaaa
    68341 actgatggca agaaaaaata cacttcgata tattttgtta gggttattaa gtaagaagaa
    68401 gatgacagga tatgaattga atcaatcgtt caaaaatgaa ataggagaat tttggcaagc
    68461 aaaacatagt cagatttatc ctgaacttgc aaaaatggaa gagcaaggaa tcattcagca
    68521 tcaagtagag atcacaggtg aaaaattgga aaagaaagtt tatgaattaa cagaagaagg
    68581 gaaggaacaa ttggcagagt ggattcatac accaaccaat gaactaccgg ttaatcgaga
    68641 cgaatttgtt ctgaaattgt atttcgtcaa agacgtgaat gatccggcat tagtagaaat
    68701 tattcagcag cagcgtgacc ttcatgaaga aaaacgactt catctattag agcgaaaaaa
    68761 aacgatcttt cctactgaaa aagaaaaaca tgaaaattac ggacactacc tgattttagc
    68821 gcatgcgatc aatcgggaaa cagaatatgc aaattggcta acagatgtat tagaagaaag
    68881 taaccgaaaa aaataaaaaa atatccgaac agtgatggag aattcctttc tatatgaaag
    68941 aaacgcagat caccgttcgg atattcttat gttcaaacag gccttttttc aagtatttat
    69001 gtagcatcta cttattaaaa agcttggtac ttaggagatt agtggcgttt agagttcttt
    69061 taacaactgt tcgtagtcag ctttttcatc gacaggaagg agcttgtaag ttaattcgtc
    69121 tgtctcgttg tcatgaatga tgtacatctt agcattctct tctttgaagt taacgatttg
    69181 taaaatcgta tagcgttcgt cgatcaattc gatatctttg attgcttttg aaaaaacttt
    69241 acggacatgg tgcattttct tttggcggat ttctgccttt tctgcagctt ttgacaaagt
    69301 aaatccgtca tctaaatatt ttttgatcag ttctactttt atgactgtag gtaaacgata
    69361 ttttctagga caattcgcat cagcttcgat cgaacgaatg aatccctttt gttcccaata
    69421 ccttaattga cgtggagaca cacctacgat atcacttaat tcactgatgc caacaaccaa
    69481 ttgatcattt tccaacaaac gtttaaggga agccccaacc atttttgtca ctccttttgt
    69541 cctcttgctt cccttaatta tatccgtatt aacttatgtg tcaagtaggt tgacaaatag
    69601 cgaatttagg actaaagtat tctagtagtt tcagaaaaaa ttcagaaaga aagagaaata
    69661 tgagtaacaa ccaacccgta gatatttacg gaaaaccata taatcggaca cttttagttg
    69721 ttgtcttatt aattggtaca ttttgtaccg ttttgaatca gaccttattg acgacagctt
    69781 ttcctacatt gatgaaagct ttcgatattt cagcgtctga tgtgcaatgg ttaacgactg
    69841 gtttcttgct agtcaacggg atcatgatcc caataacagc atggctgatc aataaattca
    69901 gttcaagaaa tttatatctt tctgccatga cgatcttttt gatcggaacg attacttgct
    69961 ttacagcacc taattttagt acattactaa tcggtcgctt gatccaagct tgcggtgtgg
    70021 gagtatcgat gccattattg caaaatatca tgctatctat tttcccgcca gaaaaaagag
    70081 gatcagcaat ggggatgtca gggatcgtta ttggacttgc tcctgcttta ggccctacat
    70141 tatctggtta tatcattgat aactaccatt ggcgtgactt gtttggtatg gtactgccaa
    70201 tcgttgttct tgtcttggtt ttagcattct tcctaatgaa aagtgttatt ccattgtcaa
    70261 atcctagcat tgatatttta tccgctattt tatcaacgat tggattcggt agtttacttt
    70321 acggattttc tagtgtagga gatgacggct ggggaagctt gaaagtaatt ggtttcttag
    70381 ttgttggtgt gattttcatc gccttgttct catggcgtca attacatttg gaacatccat
    70441 tcttggaact tcgtgtattc aaatcatcta cattcacgat tgcagctgtt ttagctggtg
    70501 taacaaatat ggcgatggta ggggcagaaa tggtcgtacc attgtatatt caaaatattc
    70561 gtggagaatc agcattccat tctggcttga tgttgttgcc gggtgcattg atcatggggg
    70621 ctatgatgcc tgtgacgggt gcgattttcg ataaatacgg cgcgaaacgt ttagccatca
    70681 cagggatgtt cattttgaca gcggcaactg caccatttgc tttcttgaca aaaacgactc
    70741 cagttttata tatcgtgatc ctttatgcga tccgtatgtt tgggatctcc atggtcatga
    70801 tgccggtaac gacttctggt atgaatgcat tgccaaataa cttgatcagt catgggacag
    70861 cggtaaataa tacattccgt caaatcgcaa gctcgattgg tacagctatt ttgatcagtg
    70921 tgttgacgaa tgtgacaaaa gcaaatatcc caagcaaatc tgtattggat aatacgccat
    70981 tagcttataa agatcaggca atcaatgcca cactgtcagg ttaccatgcc gcgtttatcg
    71041 tagcgatcat ctttgggatt cttggcttta ttattgcctt tttattgaag aaaaaagaac
    71101 ctgttctaat gaaagcgggg gataatgcat gattattgga gttttgattg tcagcttgct
    71161 tgcttttgct ttaacgaacg tgttcgctaa aaaaacatgg caaacctttt tatctttgat
    71221 tttcggacta atcttcgtcg cttcactaag tttgatcgtc gctaatttgt ccaatcactt
    71281 tggaatggaa aaagtaacgg aaacaaaaac agaaaaaatt gtttccagtg ctgattccca
    71341 aggagcagat atgcttttgt ataaagcttt agggaatgga aaagaaaaag tgtatcttta
    71401 ccgaacaaat gaaaaacaag aaaaaccaaa agcaactggt acggataacg aaacaaataa
    71461 agtagaaaag acagacggcg atgctgaaaa agtgacgaaa actacttatt gggaatacaa
    71521 aaacgacatg tataaattct ggttcaatat tgctgataac aatcacgaat atgacagccg
    71581 agtgaataca ttcaaaatcc cagagacatg ggtagaatta agtacggacc aagcagccaa
    71641 gttagctgaa ttagtgaaga aacagcaaag tacgatggaa agtgaagcaa aagcttatgt
    71701 tcaagacggt atggtaaaag caatgactga aaatccaaaa atgagcaaag cagagcaaga
    71761 acaacgtacg aaagccttag ctgctgaatt ccaacaacaa gcattcgcaa aattagtgaa
    71821 agaagcaaaa ggcgaataat attttgaaca ccatgtagga ggttgtgaaa aagctgctct
    71881 gcatcaagca gtaagaacaa tcagataaaa atagcactcc atatttttca tctgattggg
    71941 cttagtgccg tagatgcaag cttttgaaca ccgtttaagg aagaggttgt gagcctgccg
    72001 tttaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gttgactatt
    72061 gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcgaaaaaa ggaactgcga
    72121 caagactttt gtcacagttc cttctttttt ttgctatgct atactagatg taaaaaatag
    72181 gagaaataaa atgagggaaa tttattcttt tgaatcaaaa atctatgctt ccgaagtcga
    72241 caagggcggg gcatatatta tttttccgta tgatataaga aaagaattcg gtgctggaag
    72301 agttaaagtc caagcaactt ttgacaatgt gccgtatgaa ggcagtatcg tgaatatggg
    72361 cgtgaaaaat ccagatggtt cagtctgcta catccttgga ctacgaaaag atattcgtaa
    72421 aaacatcgga aaagaaatcg gtgatattgt agcagttaca gtgaagcaaa aataaaagag
    72481 gttgtgacaa aagacgtgtc acaacctctt gataaacggt gttcaaaagc cagtttctcg
    72541 gtagtaagtc aaatttatgc aaaaatgtgg aaacccatgt ttacaaaatt tttcttacta
    72601 ctcgaaacga agcggcttta tcacaacctc ttgataaacg gcgttcaatc agctgtccct
    72661 cggtattagc tcaaaaacgg caaaatatga gaagctattt ttccattttt ttctctcata
    72721 ctcaggtcat aacgctgatt tcacaacctc ttctttcata atggtttttc accatcttta
    72781 taataaactg gttctttgat atggaaatct attttttcca accaatctaa aatcttgtaa
    72841 cctaactctt ctgacatcgg aaaagaggcg ttgatttgtg cattggagat atggtcaaca
    72901 tggataccgg aagtgatcgt acaacttccc ggaacggcag ttttgatgat cacagctatt
    72961 ttttcggcta agacatcgtc tttatggaac cgaccatcgt ggctagggaa acgtacagtt
    73021 tgcatctctg tatcacgaga aaatgtagtg actgttccga tatgtggtgt atctccgcct
    73081 gttaaagtga tcaataagtc tttaccgatt actttaacag cagcttggat cgtgaaacct
    73141 agtttggtta cttcaactat cgtttctttt tccatattaa tcaccctgcc aacgttttga
    73201 atgctcattt acaattccaa attgatccaa aagtttcatt gtatggtgat taacaagatc
    73261 ttggatcgtt tgaggctgat tataaaaagc aggaattggt ggtatgattt gcacacctag
    73321 tcgtgctaat ttagtcaagt tttccaagtg aatggcattt aatggcgttt cacgaggaac
    73381 aatgactaac tttcgttgct ccttcattgt tacatcagca gcacgtgcaa tcaggttgtc
    73441 gcctaaacca acagagatac ctgcgactgt tttcatactt gccggtacga tgatcattcc
    73501 atcggtcaaa aatgatccgc tggcgacagc agcacctaaa tctttgtttg aataatggta
    73561 atcaaacagg ctttcgaatt cagttagtga taagtcggat tccagcttta agttctgttt
    73621 tgcccatgga ctgatgattc cgtgtgtttc tatcatcgga tattctttta gtttcttcac
    73681 taggttgatg gcataaatgg tgcctgaagc gccacttaca ccaatcacga tttttttctt
    73741 ccgttgtgcc aattgttcca ttttccgcac tcctttttat gatgtcaaaa cagttgcaga
    73801 tcattcatta tttcaaatat ttttcccaat cttctacttc ttggaatttt gcacgtacaa
    73861 aatgttcttt catatcgaaa ggaactgtac cgtcaaagat agtttttgca ctcatcccac
    73921 ggaaacgaat agagccagga ttatattctg gtaattctga tgggtccaat ggatggttcc
    73981 tcattcctgg gatcacgatg atatctttat ctccttggaa acgagtgttg aaagtccaaa
    74041 ctacatcgtt catgtcgaaa atatcaacat cttcatcgac taaaatgact gtcttcaatt
    74101 ctttgaaggc tgataaagca agaatagctg cttgacgctg aataccttca tccgcttcgt
    74161 tttctttacg gatttgcatg atggtcatca atttaccacc acctgcagga ggattgtaga
    74221 catttactac ttttcctgga atagctttat cgaccaaggc aagaatactt gcttctgtag
    74281 gaataccggc catagatacg tgttcttcac tgggaccaat cgttgtttgc atgattggat
    74341 tgtcttttcg gtgagtaact gctttgactt tcactacttg tacagctgga ttagcatccc
    74401 cagtatagcc aggaaattct ggcattgctt ttcctgagtt cgtgttgata tcttcttgca
    74461 ttgtttcatt tggcatgatt tctgcttcaa tgatgaattt agcacgagca atacccactt
    74521 catcgactgc tataccatca acgatttgaa taggttcgtt acgcaaagct cctgcacacc
    74581 atagttcatt gtagccaagt ggagtggtag gtggttcgaa agtcgtgcca atggcaattg
    74641 ctggatctaa accaatattg atagtgatag gcattggttt attcaaggct tcgaattgtt
    74701 tttggaagtg accaatgtgg cgtccgccag gcatgatgta cataccgatc gtatctttat
    74761 cttctagaac catacgatgg atcgttacat cgctcattgt tttttctgga tctgatccta
    74821 agactacacc cattgtgatg tatggaccag catcttcagg agtatttgta ggtgcaggca
    74881 aaatgttgcg aatatcgaag tctgcatcgg tagctagatg aacgacttct tgtgctggag
    74941 cgtctgcttt gtctactttc acaggtttga ttgggttttc tacagcgtct ttcaagaaac
    75001 gaccaagtgt tcgataatca tggtgcaaaa tttcacctac acgtttacga ctagccattg
    75061 ttccgatgtt cacacgtgtg ccggggaaac ctttgatatt gttgaaagtc atagctggtc
    75121 cttcttgagt cggacgtttt actgtaccgc cagcaccgat ataacggtaa acaccagata
    75181 attctgcttc aggatcgatt tcaatatctg tttcgtgata ttgacctgga agtgttttta
    75241 gttcttcgat gacttttctt aaatcatacg gttgatctgt catgagagat tcctccttag
    75301 ttgtctttac ctatctatag taaaaggtga aaaagaggaa aacaattcaa cttgttcggt
    75361 aaagtattct acaatggaat ggatgcgttt ttttgataat agaataaact atgatatact
    75421 tatcactaaa agaaagcgca tacttagtgt tggaggaagg aaagtgaaaa aaatgaagga
    75481 gtcatttcct aaagcagtaa aagtggaaaa tatagcgaat attttgaaag ttacatttga
    75541 aaacggagaa gtaaaatacg tgaaatctca ttggacagaa gagataaccg atgccttgca
    75601 atttggaaaa aaaggaagag gaaaaagaaa aaatcttctc gcactttctc gtaatatgtg
    75661 gattgggact gaagtaacga ttgaagcaga tggaacagta ttcataaatg gaaaagacag
    75721 gtatacgcca gaagagttgt ggtacaaagg aaagaaaagt ataccagaat tataaaaaat
    75781 aattaaaaaa aacagcgcga aacaaggaac atattacttc ctcgtttctt gctgttttct
    75841 actatttcag cgctcttatg actcaaatct aaatgtggtt gtcacaacct tattctgcac
    75901 gcaaagcgat agcggcgtct tttttcgctg ccattcgtgc aggaagatga ccgccaagca
    75961 tcgtcaatat agtagagacg atcactagaa taatcgcatg gacaggattt agttgagcaa
    76021 cattttttag atccgtcatg ttgtataaga tgctattgat agggaacgta gctagccatg
    76081 caataaacac acctaatatc ccagaagaaa tacctaaaat acacgtttct gcgtcaaata
    76141 cgcgagtgat gtctttttta cgtgcaccta aagcttttag tacaccgatt tcttttgtcc
    76201 gttcaatcac agatgtataa gtaatgatac tgatcatgat catacttgtt actaaagaga
    76261 tccctgcaaa agcaataagg acataagtga tagcatccat caatccgcca gtcagttcag
    76321 tcatcgttcc agctaaatca gtataaatga ttttatcttc atctgatttg cctttattgt
    76381 aatcatctag ataatccaag atcttttctt tgtcttcaaa attgttcgga taaatcatga
    76441 tacttgaagg caagctgtct ccacctaaat aactaagcaa tgtttgtttg gcattttcgt
    76501 caactttttc agtagttaag acatttacac cactgtctct ttgagctttg acgatttctg
    76561 attctttgtt ttcgttgacg atttgagtcg ttaattgatc gctataagcg attcctggag
    76621 ataagagatt cattgtagaa gaagatttca cgcgtaggat ccccgagatc ttcaattcat
    76681 cagaagcatt ctgatacatc gcatcataat ctgtattagg gataaaattc ccagttggaa
    76741 gttttgtata gtaagtattg ttgtttacta atttgaatgt tgtaccaacg atatcatcaa
    76801 aatcaagttt ttcgtcttct ttcacatcaa atccaagatt cttcaatgcg ttgatattag
    76861 tattattatt gccgtctacg atcagcacga catctgttgc tgaggctgga taacttccgg
    76921 caagtaaaga ataattgtct tttaaaaagt tttctttgct agtgtcaagc tgtgtaggga
    76981 aacttgacac tcccacacct gtcattgctg acatcgtact agataaagaa agagattccg
    77041 tatcaggatt ttgattcgag aagctgacag gctgtacttt gccatttaca tcacgtaaaa
    77101 gattgattcc tgtagtacgg gtaaatccga tattattact taagttagga tcgatgtctg
    77161 ttacgtaatc gacgtatgtt tgatcaatct tattcgtgtg ctgtgcttta tcttcttcgc
    77221 tgacttttgc agtaatcgtt ttgctgtctg gataatccgc tttgcttgca cccaatccgg
    77281 catcgtcacg agtctggtta gtcgttacct tagaaatagt aatcggaaat ttcgccatcg
    77341 tttcagcttg cgtgttatcg atttgttttt ggaatccaga agaaagggac agaacgatcg
    77401 cgattccgat gatcccaata ctggaagcaa aggaggtcaa gaaagtacgt cctttttttg
    77461 ttcggatatt gttgaaggaa agctttaacg ctgtccaaaa actcatcttt gttctgcgta
    77521 agttaaattg atcatctttt ggtcgttcga tgtgaggatg ggaatctgtt aagatcttac
    77581 catcagaaaa ttcaatgatc cgatctgcgt attgatgggc cagttcagga ttatgtgtga
    77641 ccatgatcac taatttttct ttcgataact cttggatcaa ttccatgatc tggatacttg
    77701 tttctgtatc taacgctccg gttggttcat cgcacaaaag gatatctggg tcattggcta
    77761 gtgctcgagc gatggccact cgctgcattt gtccaccaga taactgattt ggctttttat
    77821 gcatatgatc tgtcaagccg actcggtgca atgcatcttc tgcccgtttt cgtttttcgt
    77881 ctttagaaac accacttaat gtcatcccaa gttcaacgtt ttcaataatg cctagatgac
    77941 caatcaagtt gtaactttga aaaatgaaac cgatagagtt gtttcggtag gcatcccagt
    78001 cactgtcctt aaagtcttta gtagactttc cgttgattac catatcaccg gaatcataat
    78061 tatctagtcc accaatcaca ttcagcatag tcgttttacc agaaccacta ggacctaaaa
    78121 tcgctacgaa ttctttttgt ctgaaagcga cagacacacc atccaatgct ttggtggtgg
    78181 tttcgccgac tttataatat ttttttatat cttttaattg aagcataatc acccttctcc
    78241 cgtattgaaa tattcggtat actaataagt atatcagaac aatgatcgta gtatacgaaa
    78301 taaatgttaa ctgaagatga actacaagaa aaaatctaga aatttttcaa aaaacagagg
    78361 gagatcaatg aatcgtattt tagtagtaga agatgatccg caattacagt tgctttaccg
    78421 ttctgtgttg gaacgagcgg gcttttcagt catcaccgca ttgaatggag aggatgcgct
    78481 gaaaaaacta gaagcgacac gtatagaatt aatcattaca gatatcatga tgccgaaaat
    78541 ggatggctat gacttgctag actcattacg tagcaccaga tttgacacac ctgttctaat
    78601 gattacggca aaggccgatt ttgaggataa gaaacgcggg ttcaaattag gagcagatga
    78661 ttatatgaca aaacccatcg atgtcaatga gatgatccta cgtgtggaag cattgcttcg
    78721 acgttcaaag atcaattaca gcagtagctt gataatcgga caaacaaaat tagagctaga
    78781 tgcttataca gtgttacaag atcagcattc gttgattctg cctcaaaaag aatttctgct
    78841 tctgtataaa ttactgtctt atcccaacaa gatcttcact cgccaacagt tgatggatga
    78901 tatttgggga ttagatacga atacagaaga acgaacgatc gatgtacata tcaaacgttt
    78961 aaggacccgt tttgaaaaga cggaagattt tcaaatcatc acagtacggg gactaggcta
    79021 taaggcggtt atttaaagat gaaaaaaaag ctattttcac aattatggat ctattttgct
    79081 agtattgtct tcatttcgat cttagtgaca ctgctgtgtt ttctcggttt tattttctta
    79141 ttgtctcgac attctatctc gcctgctgaa tcggctggaa aaccaacgtt atacccgtta
    79201 ttcgtctttg cggggttcag tatggtcgtg ggtacaggaa tatcgatttt tgttgggcgg
    79261 cgtatcctgc atccaatcag tgcgctaggg acaaatatga gtctggtagc aacgggagat
    79321 ttttcgattc gaatggatga acagcagaaa gtagcagaag tacagcaact gtataaagat
    79381 ttcaacgtta tggtccaaga actcaatagt atcgagacac tgaggaatga ctttgtgtcc
    79441 agtgtatccc atgaattcaa aacaccacta gcaacaatcc aaggatatgt tcaattgtta
    79501 caagccccaa atctctctga cgaagaacgc caaatatttt tatatcggat tatcgaaagt
    79561 atcacgcagc tttcacaatt aacagagaac atcttaaaat tgaacaaact ggaaaaccag
    79621 cgtatccaac tggaaaagaa agagtatcga ttagatgaac aaatcagaga agtaatcgtt
    79681 tttttgcagc ctaaatggga gaaagaacag ctagaattgg atatcgaact tgctgcagta
    79741 aattacacag gcaatgaaga gtttttgtac caagtgtggc tgaatatcat ggataacgcc
    79801 ataaagtaca atcaaatcaa tggacagatt catatcaaac tatttgagac agccacagag
    79861 attgtactgg aagtgaccga ttcaggtgta ggaatgaatg aagagaccag agatcgaatg
    79921 tttgaaaaat tttatcaagg agatacaagt cgtcaaattt ctgggaatgg gctaggactt
    79981 tcactggtta aaaaaattct agaactgcat gatggacgta ttgattatag cagtatcgaa
    80041 ggagtaggaa cgacagcaat gatacggtta agtaaacaat aggaaaaaga ataaaacccc
    80101 taaaaattct attgaaggtt tttaggggtt tttagtcgtt tttttgtgat tagggaaatg
    80161 ttcttacggt tcttttggca taaaaatcgc tgctttctta ttgaactacg agaaatgtta
    80221 gaggtgtcat tgaaacaagg acatgatgca agaaagagag cgaaagtcat gcgtcgctct
    80281 ctttttttgg gtggatagat ggacagaaaa tgtagggctt agttttcttc ttgatttaaa
    80341 tactcccaaa atgtatctga agccaaagaa tgctcaccga ctttacgagt aaatgcaaga
    80401 taactctctg ttgttggaga aatagggaga atcacagtat tttcttttaa tagattcttg
    80461 actgtttttt ccataacaat agcaatcccc atgtcatttg caatcatatt gatgatcaag
    80521 tcgattcttg ctcctttata agagattttt ggttcaaatc cagcttcgcg acacagatct
    80581 agtacaggtt gatacaagtt cgtagtgggg ccaaggagca aaaagttctc ctttttcagt
    80641 tcgcttagat ccaatttctt tttactagct aaaggatgat ttttaggtag aactgctaca
    80701 aaatcgtctg tttcagtagg caaccactca atcgaacttg gtggggcctg gaaacttcta
    80761 gcaaagtgga tcgtgtcact atgttcattg gcgtcattgt aagttaattc gacactttcc
    80821 acttctttca actggactgt aaattcagga tggcgtttca aaaactcggt gattttggaa
    80881 aaacctttat aattgatcat tgtcggtatc gttaagatat ttaatgaaag attgttgtct
    80941 ttcgtatagg tttcaagcac ttcttgcata gcatggtatt gttctacgac attttttaca
    81001 tatgggagca ataagcttcc agcttcggtc aattcgattt ttctatgggc gcgttcgaat
    81061 aatttgacgt ctaattctgt ttctaacgaa agaatctgtt tagaaatatt tccttgggtg
    81121 gtgaattgtc gatcagcagt tttggtgaaa ttcattgttt cggctaaatc aagaaaaact
    81181 tcgaatttat ggatatccat catattcctc ctacttattc caataaagaa tactttaaca
    81241 caaaaaatga attggtgtaa tcttctctgt ctgttacaat ctttcttgca gcgaaagcaa
    81301 taattacaga aatttttctg atttttttcg cttcgtagaa aaagagagga gttttttgat
    81361 gccaactgga gttttgatca atgtaggttc cgttttgctt ggcggactaa ttggcggatt
    81421 agtaggaaat aaattaagtg aacattttaa agcacagctg acgatggttt ttggtgtgtg
    81481 ttcgatgggg atgggaatct attccattgc gccgatgaaa aatatgccgg ctgttatttt
    81541 tgctttagtg ataggaacag cgattggtct gattgttcat ttaggtaatg ggataaataa
    81601 gggggctgcg ctgatgcaag tacctatttc taaaatcttc cctagtgaaa agcttggtat
    81661 gacccacgat gaatttatca gcacattagt gacggtcatt gttctatttt gtgcaagtgg
    81721 tacggggatc tatggaagtt tagattctgg gatgacagga gacagtacga ttttgatttc
    81781 taaatctgtc cttgattttt tcacagcagc tatttttgcg tgtaatttag ggtatgtcgt
    81841 ttctgtgata gcaattcccc aattcattat tttttatatt ttgttcttat tggcaaaatt
    81901 tatttatcca ttgactacac cagacatgat cttagatttt aaagcttgcg gcggattctt
    81961 gatggtcgct accggtttta gaatgatcaa cgtgaaaatg ttcccagtag ctgacatgat
    82021 acctgcaatg atcgttatca tgccactgag ctggatgtgg acaaattgga ttatgccact
    82081 gttataataa aaatcaaaga gaagccattt tttgctgact aaaaaggatc aaatctttta
    82141 gtcagttttt ttgtatttga caaactgatt ttgaaactat attatatgtt gtataatata
    82201 gattgaaata aaatattgca cttgagaata gaaggtgacg taatgcacat ccagattcca
    82261 actgttcttc ttgatggtac tgtgctagca gcattaagca aggaagatat gtatgggtat
    82321 gccttaacaa aaatcgtaca agaaagtctc tctgtcagtg aatctacact atatccggtt
    82381 ttgagaagat tgaaaaaaga aggatatctt gagacctatg atgaaccttt tgaaggcaga
    82441 aaccgtcgat attactcgtt gacaccaatg ggaagtcgtc atttagaaga aattattgat
    82501 gaatggattg tttttcgaga ttctttagac cggttattgg aggagagcac aaatgaatga
    82561 atatttgaat gaagttgtgg atcatcttca agaaattcct gaagacgatc gttttgattt
    82621 gatccagtat tatgaggaat attttttaga ttctggaaaa acattagatc agatcattga
    82681 tgaatatggt acaccaaagc agtttgcctt aaaattaaaa atcagttact tttcagaaga
    82741 agaacaagca gaaaacgagt tagtgaaaaa tccaaaaaga caaattcgcc tgatttggtt
    82801 aatcattatc ggtttatttg cttctcctgt cctcattcca ctagcactgg ggtttatatt
    82861 ggttattggg ggactattgg ttgctttact agcgctcatt tttagtatct atgttttatg
    82921 tatcagtatc ttaggtgttg gcctattcat gatcatctca ggaatcagtg tgtttggaca
    82981 atcgattctg agtggattat tctttatcgg tgtcggtctg ttcgccacag gaagcgcagt
    83041 atttatttcg ccactgttgc tgaaaggaac aaaagggctt ttttatgtca tgatgaactt
    83101 tgttaaatgg atgggacgac gttttattac aaaaagagat ctgcattcag cgggcgtttg
    83161 aaggaggaca cataaattga gattgaaaca ttttttgatt accggtattg gtctgatgct
    83221 tactggtggc ctcctagcag gtgcaactta tacattaggc gctcaaaagt gtctgacttg
    83281 ggataatggt cctcggttga tcgatatggt tactgagaca aaaaaaatca aggatgcaaa
    83341 caaaatcgtc atccatgcag aaaatcaaac agtttcggtc atacgcggat tggattttga
    83401 gataaaaacg acttatgata agacagaaaa gccagaaata aaggagaaaa atggcgtgtt
    83461 gactgtgaat gcagtcaaaa aatcaacagg tgcaatcatt agtttagaag acacaagtgc
    83521 acaaatcact attgttgtcc ctgcagatca tgaattatct gaattgacaa tcgaagggaa
    83581 taatagtgca attgatatca acgaagtatc aatcaaaaaa gtgaatacta agacacatga
    83641 tggctgggta tcgttcaatg aatttacagg aggaacaata actgcgaaag atgaattagg
    83701 ctcacttcga ttagatgagg ccaatgtgga taacttgatc gtcacaggtg aaaacagcaa
    83761 cttccatcta gaagacagtt tgatcagcaa agaaggaaaa gtcatcctaa acggtggcaa
    83821 tttatctttg catgagatgg aaaacagtgg ctatactatc caaacgtctg gtgaatctta
    83881 tattgaaaaa gactaccagc caataacaaa tactttgtta gaacaaggac agaaaaagct
    83941 aaagatcact gctaaagaca catcgatcaa tattacgacg gatcaagagg acaatgaaac
    84001 agattgatcc tgtactcaac tggatatttt aagaaaaaac agcaaagatc cttccaatct
    84061 ttgctgtttt tcatcgctta acgcacataa ctatagtaaa ataagaaaga aaatatctaa
    84121 aaagaaaaca gggtgaaaga gtatatgaag gtagaagaag gtcaaagaga aaagttaaaa
    84181 acagaaatgg aaagattaca tacttatatc actcaactaa gccaaacctt ctatgatcca
    84241 gacaaagaaa aagtgatggt taattacccg aacaattcag aaggtcgcca attagagcaa
    84301 gtgtaccatg aagttttcaa acatttattg acggtgaaga aagagctgga ttattattct
    84361 ttaccgatta tcgatacagg aatattgaag tatgatggga agaaggaacg atttatcttt
    84421 aaatctgttc gggaagattt gcctctttca gcagggatgg atctagaggt cttggtagaa
    84481 gactatttta cagaagaaaa gcactgggtt cgaacaaaat tggattattt accgcaggca
    84541 gccggaggga cacaggcaag cggttggtac atcactgaag acaaagagtt agaactagaa
    84601 ggtgtaatgg cgcgtattcg aaaaaaaaaa ttaaaaaagt aacattagat atttattgaa
    84661 ggataaagga gcgcaggaag tagcatgaga gaaaaaagag cattttatat caatggttat
    84721 gcagggattg tcggattggt cgtattagct ttagttggat tgtttttctt ctatttaggt
    84781 atgtggcaag cgaaagtttg ggcacttttt ttaagtattt ttctttggtt gatcacgtta
    84841 ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag caaaagtaat tttgtttttg
    84901 gacaatattt aggtacgatt cgagaaaatg gctttttcct tacgatacct ttggcacaaa
    84961 aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc tgttttaaaa gtcaatgatt
    85021 tggatggaaa ccccatcgaa atctcagcag tagtagtctt taaagtcgtt gatacagcaa
    85081 aggcattatt cgatgtagcg tattatcaag attttgtaga aatccaaagt gagacagcca
    85141 ttcggcatat cgctagccaa tacccgtatg atacattcaa tgagaatgat ttgacgcttc
    85201 gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga gctgcaagag cgtttagcag
    85261 tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt agcctatgca acagaaatcg
    85321 ctagtgccat gctccaacgt caacaagcaa aagccatttt gtctgctcga caaaccattg
    85381 ttgaaggagc tgtcacaatc actcaaatgg ccttagagca aatcgaagat ggacaagaaa
    85441 tcaatttcac cgatgatcgg aaagtccaat tgatcaataa tttacttgtc tcaatcatta
    85501 cagacaaagg aacgcaacct gttatcaata caggcgacat gaaagaccgt aagtgacata
    85561 ctttttgtaa gataggaaga aggagtttag gacggattat tgtcttgaac tcttttttta
    85621 gttgttttaa agaaaatgac aaaattgaaa gaagaatgat agttgattcg aaggcagaat
    85681 tgttcgcttt tttctataat cagtatataa agtaagagag aaggggaaaa agtgaaaaaa
    85741 cttctgatcg gtctacttgt tttcatcgtt gtggtacttg caggggtgaa aatcgcagat
    85801 tacgtagtta tgggtggaga agaatactat gtacaaatta cgacagatgg tacaaaaaca
    85861 gtttcaaaat cagactctgg acaagaaaca gtcaactata gatatatcct gcctggctat
    85921 acgaaagagg gtaaggaaaa agaactggac ttcaagggac aattgactcg accgcttcgt
    85981 aaaggagcat atttaaaaat cacctggaac aaaaataaag gtgtcacttc gtatgaagaa
    86041 gtcgaaaaaa aagacgtgcc gaaaacagca ctggaaaaac taattaaagg gtagggaaag
    86101 aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt atacggaaaa aacaatgaga
    86161 aaaagacgca agcattaagc ggcatcagct tcgatgtaga aaaaggcgaa tttattggga
    86221 tcatgggcgc ttctggttct ggtaagtcga cacttttgaa tattttatcc acattagata
    86281 aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt gaccacatta aaaggaaatc
    86341 aactagcaga ttttcgtgcg aatgagattg gctttatttt ccaagatttc aatttattag
    86401 aaagcttgac agcacaagaa aatatcgctg ttcctctttc tttacaaggc gtcagaccaa
    86461 aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt atccatctct catattttgg
    86521 aaagctatcc ttctgagatt tccggtggac aaaaacaacg ggtcgccgcc gcacgtgcat
    86581 tgatcacaca accaacgatt ctattagggg atgagcctac aggtgcactg gattccaaaa
    86641 gtgcccggga tcttttagat acgatggacg aattgaatac aaaagatcat gtatcgattc
    86701 ttcttgtgac gcatgatccg ttttctgcaa gttattgcca acgtatttta ttcatcaagg
    86761 atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag ccgtgattcc ttctatcgag
    86821 aaatactatc tattcttgga aacttggaac agtaggggga gattgactat gctttggaaa
    86881 ttatcactta ctgggatcaa gagccgttta agagactact tggtgttatt ctcaggatta
    86941 gttatggcat ctgctatttt ttacatgttt gaatcaatgg caagcaacga agcttttttg
    87001 aaaagtaata cgatcgttgc ggctacagtt atcattttcc gattcggttc tgttttacta
    87061 gggatcatca cattcgttta tattttatat gcaaattcct ttttgatgac gatgcggcaa
    87121 aaagactatg caatgtttat gatgctggga gcaaaaggca gaaaaatcgc acagatgatt
    87181 ttttctgaaa cattcttagt agggatcgca gctactttag tgggttcagc tgtcggtgta
    87241 ggattgacaa gtatcgtcca ccgattatta gtggatcagt tagatctcaa tatttcgcat
    87301 tttacacctt ttagtattca cgggttgttg atcactgttt tgttttttgc tgttttgttt
    87361 ttattagctg ctgttgtcaa tgctttttcg attgtcaaaa aaccgatttt agcattgatt
    87421 cgtgcggatc agacaccaac aagaatcaag cagcataaat tcttgttttt ccttgaagta
    87481 gtactgggaa ttgcttttct aggctgcggt tactatatga tgaaacacgt ggggacctat
    87541 cgagtctttg gtataggggt agccttagtt acgatcgtat taggaactta tttcattttc
    87601 cattctgtga tcattttctt tttatcatta ctaaagaaat cagaacagat ttcattgaaa
    87661 aaattgaaca atttcacatt gtcacagtta aactttcgta ttcgtgaata tacacaaatg
    87721 ctctcaatgg tcgctatgct gtttgccttg gcactgggcg ctttgacggt aggattaggt
    87781 tttaaaaatg aaattgcaga cttgacagaa aacaattctg gatatgatct agtgatgaat
    87841 aatcctcggg cagaagatca gcaaaaaatc aaagaacttt caccaacact ttctgcttct
    87901 tatactcaaa aagaagatgc gggaacgatt tactataatg caagtgaatt tgcggctcaa
    87961 ccattgatca tgataaaaca tgaagatgga acaccgccga agatcacgaa agaaaaagtt
    88021 cctgctaaaa aaatgaatga actgactgta caagaggaac ttcgaagcta tgaattgccg
    88081 gaacagcaag aaaaagaaat caaggtagtc agtcaatcag aatttgatca actgaacttg
    88141 ccacaatcca ctttacagct agttcaggta aaagacttcc aagcaaacct gaaaccaatc
    88201 gaaacacttg ttgctcaaaa taaaatgaac aatccatctt tgcaaaaagt agagtacagc
    88261 aaccagaaat atgaaatgta tgaagtgtat aatggtctat tctctggttt tgaatttatg
    88321 ggattcttcc taggtttagc tttcttgacc atgcttgcta gctgtttgat gttcaagatc
    88381 ttgtcaggaa gcaagagtga cattgctcgt tatgaaatgc ttgaaaagat aggaacacga
    88441 cgcgggttgc ttaaacaatc gatccgtaga gaagtaggcg tgttattctt agcaccagga
    88501 atcttaggtg tagtccatgt cttattcgga ttacaaatgt ttggtctatt catgcaaaat
    88561 ccatacaaag atatttgggt tccgtttacg atcttcttcg tattgtattt catctactat
    88621 gtactgacga catggttata tactggaatc gtattgaaaa agagattgtg aaaaagctgt
    88681 cctgcatcaa gtaataagaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt
    88741 gggcttatta ccgcagatgc aagcttttga acaccgtttg ttcggaaaaa gaggttgtga
    88801 caatattttt gtcacaacct ttttttcacg gtcttttatt agaatatagt aaattttttc
    88861 tcgttactca cggaataagc tgttaagaaa acggtttcca tgtatactgg aatcagtgca
    88921 aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg aacaaaattt tggcgaaaac
    88981 tatcacttga ttacgatcac gaatcaaaat gggacatctt tgtctgtcag tgatcttggg
    89041 gcacgggttg ttcattttca aacaataatc gataatgaaa aacgagagtt gatcttaggt
    89101 tttgatgaag caaaagaata tctagaaaaa gacccttata tcggtgcttc aatcggacgt
    89161 atggctggac ggattacgaa tggcacattc aaattagatg atgaaacgtt cgaagtaaaa
    89221 acagacccgg acactggtca ttgtttacat ggtggtgcac ctagctttga agccaagcag
    89281 tggcattata cagtcatcaa tggtgaaaac gaagcatcgg tcattttcta tcagactagc
    89341 ccagatatgg aaaacggatt tcctggaact ttagatgtag aagtacggta tacgctgacg
    89401 aacgatgata catggcgagt gacggtccaa gggatcagtg accgcaaaac agtattcaat
    89461 cctacaaatc atgtctattt caatttaacc ggtgatccag cacagtcaat cgacgaacat
    89521 atactttggt tgaatagccg attatttgct acgctcaata aagatacaac gcctacagga
    89581 gaagtagttt cagtaagtgg cacagcattt gacttccaac gtcctaaaaa attaggtgag
    89641 gtctttgcca gtgattttcg ccaatcaatg atagtgggcg gcatcgatca tccatttttg
    89701 ttgaaaaaac aatctggttt actaaatgct gcggaattga caagcccaga tgaaaaagta
    89761 tcgatccaag taaaaacaga tgcttcagga atcgtgattt ttacagcaaa ttttggagaa
    89821 gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg gcggtatcac atttgagact
    89881 caagaactgc caggagcgga gcgacttgaa agttttggag atgtcactct ggaacctaac
    89941 caagtacgcg agtttgtaac ggaatacaaa ataaatataa agaaatagaa taaat
//
LOCUS       DANJMA010000005        84971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000005 DANJMA010000000
VERSION     DANJMA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..496)
                     /gene="mreD"
                     /locus_tag="PWU31_000409"
     CDS             complement(<1..496)
                     /gene="mreD"
                     /locus_tag="PWU31_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL1082980.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKL"
     gene            complement(500..1354)
                     /gene="mreC"
                     /locus_tag="PWU31_000410"
     CDS             complement(500..1354)
                     /gene="mreC"
                     /locus_tag="PWU31_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL1082981.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(1509..2147)
                     /locus_tag="PWU31_000411"
     CDS             complement(1509..2147)
                     /locus_tag="PWU31_000411"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL1082982.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(2148..2798)
                     /locus_tag="PWU31_000412"
     CDS             complement(2148..2798)
                     /locus_tag="PWU31_000412"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL1082983.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(2811..3710)
                     /gene="rsgA"
                     /locus_tag="PWU31_000413"
     CDS             complement(2811..3710)
                     /gene="rsgA"
                     /locus_tag="PWU31_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL1082984.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(3875..5944)
                     /gene="pknB"
                     /locus_tag="PWU31_000414"
     CDS             complement(3875..5944)
                     /gene="pknB"
                     /locus_tag="PWU31_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL1082985.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(5941..6681)
                     /locus_tag="PWU31_000415"
     CDS             complement(5941..6681)
                     /locus_tag="PWU31_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL1082986.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(6694..8052)
                     /gene="rsmB"
                     /locus_tag="PWU31_000416"
     CDS             complement(6694..8052)
                     /gene="rsmB"
                     /locus_tag="PWU31_000416"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL1082987.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(8052..8990)
                     /locus_tag="PWU31_000417"
     CDS             complement(8052..8990)
                     /locus_tag="PWU31_000417"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL1082988.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(8983..9474)
                     /gene="def"
                     /locus_tag="PWU31_000418"
     CDS             complement(8983..9474)
                     /gene="def"
                     /locus_tag="PWU31_000418"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1082989.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(9489..11897)
                     /gene="priA"
                     /locus_tag="PWU31_000419"
     CDS             complement(9489..11897)
                     /gene="priA"
                     /locus_tag="PWU31_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL1082990.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(12150..12455)
                     /locus_tag="PWU31_000420"
     CDS             complement(12150..12455)
                     /locus_tag="PWU31_000420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1082991.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(12460..13074)
                     /gene="gmk"
                     /locus_tag="PWU31_000421"
     CDS             complement(12460..13074)
                     /gene="gmk"
                     /locus_tag="PWU31_000421"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL1082992.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(13561..13845)
                     /locus_tag="PWU31_000422"
     CDS             complement(13561..13845)
                     /locus_tag="PWU31_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082993.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(13863..14753)
                     /locus_tag="PWU31_000423"
     CDS             complement(13863..14753)
                     /locus_tag="PWU31_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL1082994.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(14773..14991)
                     /locus_tag="PWU31_000424"
     CDS             complement(14773..14991)
                     /locus_tag="PWU31_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL1082995.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(14961..16661)
                     /locus_tag="PWU31_000425"
     CDS             complement(14961..16661)
                     /locus_tag="PWU31_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL1082996.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16928..17701
                     /locus_tag="PWU31_000426"
     CDS             16928..17701
                     /locus_tag="PWU31_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1082997.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(17782..18351)
                     /gene="thiT"
                     /locus_tag="PWU31_000427"
     CDS             complement(17782..18351)
                     /gene="thiT"
                     /locus_tag="PWU31_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL1082998.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(18417..18519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18525..19496)
                     /locus_tag="PWU31_000428"
     CDS             complement(18525..19496)
                     /locus_tag="PWU31_000428"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL1082999.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(19590..20963)
                     /gene="glmU"
                     /locus_tag="PWU31_000429"
     CDS             complement(19590..20963)
                     /gene="glmU"
                     /locus_tag="PWU31_000429"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL1083000.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(21466..22284)
                     /gene="purR"
                     /locus_tag="PWU31_000430"
     CDS             complement(21466..22284)
                     /gene="purR"
                     /locus_tag="PWU31_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL1083001.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(22451..23275)
                     /locus_tag="PWU31_000431"
     CDS             complement(22451..23275)
                     /locus_tag="PWU31_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1083002.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(23248..23928)
                     /locus_tag="PWU31_000432"
     CDS             complement(23248..23928)
                     /locus_tag="PWU31_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1083003.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(23951..24889)
                     /locus_tag="PWU31_000433"
     CDS             complement(23951..24889)
                     /locus_tag="PWU31_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL1083004.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(25134..25997)
                     /locus_tag="PWU31_000434"
     CDS             complement(25134..25997)
                     /locus_tag="PWU31_000434"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL1083005.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(26139..28712)
                     /gene="mprF"
                     /locus_tag="PWU31_000435"
     CDS             complement(26139..28712)
                     /gene="mprF"
                     /locus_tag="PWU31_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL1083006.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            28853..29659
                     /gene="proC"
                     /locus_tag="PWU31_000436"
     CDS             28853..29659
                     /gene="proC"
                     /locus_tag="PWU31_000436"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL1083007.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(29738..31294)
                     /gene="rny"
                     /locus_tag="PWU31_000437"
     CDS             complement(29738..31294)
                     /gene="rny"
                     /locus_tag="PWU31_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL1083008.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(31705..32754)
                     /gene="recA"
                     /locus_tag="PWU31_000438"
     CDS             complement(31705..32754)
                     /gene="recA"
                     /locus_tag="PWU31_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL1083009.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(32863..34104)
                     /locus_tag="PWU31_000439"
     CDS             complement(32863..34104)
                     /locus_tag="PWU31_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL1083010.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(34344..34922)
                     /gene="pgsA"
                     /locus_tag="PWU31_000440"
     CDS             complement(34344..34922)
                     /gene="pgsA"
                     /locus_tag="PWU31_000440"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL1083011.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(35131..36054)
                     /locus_tag="PWU31_000441"
     CDS             complement(35131..36054)
                     /locus_tag="PWU31_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083012.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(36793..38079)
                     /locus_tag="PWU31_000442"
     CDS             complement(36793..38079)
                     /locus_tag="PWU31_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL1083013.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(38072..39340)
                     /locus_tag="PWU31_000443"
     CDS             complement(38072..39340)
                     /locus_tag="PWU31_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL1083014.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            39592..40053
                     /gene="mscL"
                     /locus_tag="PWU31_000444"
     CDS             39592..40053
                     /gene="mscL"
                     /locus_tag="PWU31_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL1083015.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(40112..40558)
                     /locus_tag="PWU31_000445"
     CDS             complement(40112..40558)
                     /locus_tag="PWU31_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083016.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(40651..40896)
                     /locus_tag="PWU31_000446"
     CDS             complement(40651..40896)
                     /locus_tag="PWU31_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL1083017.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(41011..41589)
                     /locus_tag="PWU31_000447"
     CDS             complement(41011..41589)
                     /locus_tag="PWU31_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1083018.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(41666..42196)
                     /locus_tag="PWU31_000448"
     CDS             complement(41666..42196)
                     /locus_tag="PWU31_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL1083019.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(42200..43060)
                     /gene="rlmB"
                     /locus_tag="PWU31_000449"
     CDS             complement(42200..43060)
                     /gene="rlmB"
                     /locus_tag="PWU31_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL1083020.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(43053..43448)
                     /locus_tag="PWU31_000450"
     CDS             complement(43053..43448)
                     /locus_tag="PWU31_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL1083021.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(43445..44857)
                     /locus_tag="PWU31_000451"
     CDS             complement(43445..44857)
                     /locus_tag="PWU31_000451"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL1083022.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(44854..45390)
                     /gene="cysE"
                     /locus_tag="PWU31_000452"
     CDS             complement(44854..45390)
                     /gene="cysE"
                     /locus_tag="PWU31_000452"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL1083023.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(45476..45715)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(45712..47172)
                     /locus_tag="PWU31_000453"
     CDS             complement(45712..47172)
                     /locus_tag="PWU31_000453"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL1083024.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(47342..48499)
                     /locus_tag="PWU31_000454"
     CDS             complement(47342..48499)
                     /locus_tag="PWU31_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL1083025.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(48533..49903)
                     /gene="radA"
                     /locus_tag="PWU31_000455"
     CDS             complement(48533..49903)
                     /gene="radA"
                     /locus_tag="PWU31_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL1083026.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(49966..50493)
                     /locus_tag="PWU31_000456"
     CDS             complement(49966..50493)
                     /locus_tag="PWU31_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL1083027.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            50723..51529
                     /gene="proB"
                     /locus_tag="PWU31_000457"
     CDS             50723..51529
                     /gene="proB"
                     /locus_tag="PWU31_000457"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL1083028.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            51526..52773
                     /locus_tag="PWU31_000458"
     CDS             51526..52773
                     /locus_tag="PWU31_000458"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL1083029.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            52849..53193
                     /locus_tag="PWU31_000459"
     CDS             52849..53193
                     /locus_tag="PWU31_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083030.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(53310..53636)
                     /locus_tag="PWU31_000460"
     CDS             complement(53310..53636)
                     /locus_tag="PWU31_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083031.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(54251..55453)
                     /locus_tag="PWU31_000461"
     CDS             complement(54251..55453)
                     /locus_tag="PWU31_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1083032.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            55570..56085
                     /locus_tag="PWU31_000462"
     CDS             55570..56085
                     /locus_tag="PWU31_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1083033.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(56284..56730)
                     /locus_tag="PWU31_000463"
     CDS             complement(56284..56730)
                     /locus_tag="PWU31_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL1083034.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(56777..58255)
                     /gene="msr(C)"
                     /locus_tag="PWU31_000464"
     CDS             complement(56777..58255)
                     /gene="msr(C)"
                     /locus_tag="PWU31_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL1083035.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(58609..59238)
                     /locus_tag="PWU31_000465"
     CDS             complement(58609..59238)
                     /locus_tag="PWU31_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1083036.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(59222..59686)
                     /locus_tag="PWU31_000466"
     CDS             complement(59222..59686)
                     /locus_tag="PWU31_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1083037.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(60173..61441)
                     /locus_tag="PWU31_000467"
     CDS             complement(60173..61441)
                     /locus_tag="PWU31_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1083038.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(61672..62409)
                     /locus_tag="PWU31_000468"
     CDS             complement(61672..62409)
                     /locus_tag="PWU31_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1083039.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(62476..63045)
                     /locus_tag="PWU31_000469"
     CDS             complement(62476..63045)
                     /locus_tag="PWU31_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL1083040.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            63173..64045
                     /locus_tag="PWU31_000470"
     CDS             63173..64045
                     /locus_tag="PWU31_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1083041.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            64387..65259
                     /locus_tag="PWU31_000471"
     CDS             64387..65259
                     /locus_tag="PWU31_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1083042.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            65256..67214
                     /locus_tag="PWU31_000472"
     CDS             65256..67214
                     /locus_tag="PWU31_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1083043.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(67546..68397)
                     /locus_tag="PWU31_000473"
     CDS             complement(67546..68397)
                     /locus_tag="PWU31_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1083044.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            68520..68987
                     /locus_tag="PWU31_000474"
     CDS             68520..68987
                     /locus_tag="PWU31_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL1083045.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(69250..69732)
                     /locus_tag="PWU31_000475"
     CDS             complement(69250..69732)
                     /locus_tag="PWU31_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL1083046.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(69832..70695)
                     /locus_tag="PWU31_000476"
     CDS             complement(69832..70695)
                     /locus_tag="PWU31_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1083047.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            71293..72168
                     /locus_tag="PWU31_000477"
     CDS             71293..72168
                     /locus_tag="PWU31_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083048.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(72313..72465)
                     /locus_tag="PWU31_000478"
                     /pseudo
     CDS             complement(72313..72465)
                     /locus_tag="PWU31_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(72733..74064)
                     /locus_tag="PWU31_000479"
     CDS             complement(72733..74064)
                     /locus_tag="PWU31_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1083049.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(74213..74926)
                     /locus_tag="PWU31_000480"
     CDS             complement(74213..74926)
                     /locus_tag="PWU31_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1083050.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            75101..76525
                     /locus_tag="PWU31_000481"
     CDS             75101..76525
                     /locus_tag="PWU31_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1083051.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            76824..77531
                     /locus_tag="PWU31_000482"
     CDS             76824..77531
                     /locus_tag="PWU31_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL1083052.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            77518..77841
                     /locus_tag="PWU31_000483"
     CDS             77518..77841
                     /locus_tag="PWU31_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1083053.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(77930..78967)
                     /locus_tag="PWU31_000484"
     CDS             complement(77930..78967)
                     /locus_tag="PWU31_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1083054.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(79288..80271)
                     /locus_tag="PWU31_000485"
     CDS             complement(79288..80271)
                     /locus_tag="PWU31_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL1083055.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(80686..81456)
                     /locus_tag="PWU31_000486"
     CDS             complement(80686..81456)
                     /locus_tag="PWU31_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083056.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(81449..82384)
                     /locus_tag="PWU31_000487"
     CDS             complement(81449..82384)
                     /locus_tag="PWU31_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083057.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(82700..84349)
                     /locus_tag="PWU31_000488"
     CDS             complement(82700..84349)
                     /locus_tag="PWU31_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1083058.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(84466..>84971)
                     /locus_tag="PWU31_000489"
     CDS             complement(84466..>84971)
                     /locus_tag="PWU31_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1083059.1"
                     /translation="NLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNVEEGR
                     FNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIELPEDL
                     IVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPPYDYL
                     SILINER"
BASE COUNT        24270 a        18065 c        15087 g        27549 t
ORIGIN      
        1 ataatttctt aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag
       61 gtcctaaaaa ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact
      121 ggaccaccat cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag
      181 taaagatgtt gacatggata acgtctttca ttacatacat caaccataca attaaaggga
      241 gagctactgc atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa
      301 caatcgtggt aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca
      361 atatcagaaa gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg
      421 ttaaatgacc atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg
      481 tttctttctt caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca
      541 cgtcatacat ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg
      601 gtttcgtttt aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg
      661 atgtctggac aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta
      721 aggcatggag cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag
      781 atacccgtac tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag
      841 acgaagccgt attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca
      901 tatttgcttc gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt
      961 caggcgaacg agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca
     1021 actcaagttc ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa
     1081 cgtcatcata gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa
     1141 gattatggac agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat
     1201 caaccattgc aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg
     1261 ctcgctgcgc agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa
     1321 taataatatt tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc
     1381 ttgtgttcaa ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct
     1441 tttttttagc aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg
     1501 tttatggatc aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct
     1561 gcggtatctg aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt
     1621 tccaatacat acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa
     1681 gctaaatact tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac
     1741 aaataatttt gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc
     1801 atacctaacc agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa
     1861 gtgacttccg cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta
     1921 tcgtcttttt ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc
     1981 tcttcgttcg acaatgaatc aaaatcaccc actgccagat ctactgggta gcctgcttga
     2041 agcaggtaca gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac
     2101 tgcggccagt tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc
     2161 tcttttaatc gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca
     2221 acgaagacat ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca
     2281 tctacttcga ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt
     2341 ttttctactg tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact
     2401 agtacttgat cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg
     2461 ataactactc cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga
     2521 ggagttgctt cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg
     2581 tagttttctg gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg
     2641 attgcctgaa caatatttgg ccctaatgtg atatttggga caaatcggcc atccattaca
     2701 tctacgtgga tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg
     2761 gcaaaatctg cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt
     2821 ttaccttctt tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa
     2881 taattgtcgt aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag
     2941 ccaggttctt tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat
     3001 tcaggaaatt gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat
     3061 cctggtgtat ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta
     3121 tgccgtcctc ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat
     3181 atttgattca gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt
     3241 tctgggaagt aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga
     3301 tagcctattt tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag
     3361 tcggtctttg tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag
     3421 cggtcaagta gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga
     3481 tcaacatttg tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga
     3541 tagccgtctg tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt
     3601 ttccgattcc ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca
     3661 tagtaaaaac cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt
     3721 gtttatttta ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat
     3781 atggcttgaa aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat
     3841 gtcgcattcc cttctgatat attttattca gcacttattt tgaactttct gaggtgctag
     3901 ctgatgaact tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc
     3961 tacttgagct gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg
     4021 gtcctttaga gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg
     4081 gtactgtacg aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc
     4141 cgatatattc tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg
     4201 agatatcatt caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt
     4261 ctgctacaac ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt
     4321 cgctatagtc ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat
     4381 tacgaacagt ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt
     4441 tgctgactgt gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag
     4501 attgcgaaat gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca
     4561 agtcagcaga aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac
     4621 tgtaatcatc taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt
     4681 tttgcttgac tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt
     4741 tgtcatctgc aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt
     4801 ctctagcaga agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc
     4861 cagaagcaaa gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca
     4921 gccaccattt tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggta
     4981 aggtttcgtt ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg
     5041 gtgcatcttc cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc
     5101 atttttcttc gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct
     5161 cggctgactt ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca
     5221 gtgcttgtgg cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt
     5281 ttaaagcaat tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat
     5341 aaaggatgat ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct
     5401 gctcagggga cagataatga acagatccta gcatcgtatt cgtctgcgtg atcgatgttt
     5461 cagataaagc gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca
     5521 gtacgttttg gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag
     5581 cagaaaggat ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt
     5641 ggatatatcg tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt
     5701 cttcttcacc aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta
     5761 aagcttctct ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga
     5821 ctttaactgc tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac
     5881 caccgctacc gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga
     5941 tcatacactg tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc
     6001 ttcattggca cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat
     6061 gatcgttcct atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag
     6121 tcgatctttt agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc
     6181 gacagaacgt gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc
     6241 tcctgatttg actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc
     6301 ttcacgaatc aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga
     6361 cagagcggcc gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg
     6421 ctgataaatc agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc
     6481 atcgtcaaca gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact
     6541 ggcaatgtct ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc
     6601 tgaacggctg gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc
     6661 tgattgatat tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa
     6721 aaatccatct gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga
     6781 ttgtagcata ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa
     6841 agcagaaaca acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat
     6901 accggaagac tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa
     6961 attcgctaat tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc
     7021 aagacctgaa caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt
     7081 actgacttct ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc
     7141 tgcattatct tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc
     7201 accgcatgac ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca
     7261 ggcatctaag acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact
     7321 ttcatcttgg atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt
     7381 tttcgctact ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg
     7441 caaaacagtg atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg
     7501 gctaggctga tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac
     7561 cagtcgtttc gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc
     7621 agcaatgcct tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac
     7681 aaatttccct gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat
     7741 agcatgtgca gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcat
     7801 cttgacccag tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa
     7861 tagtcgctga tggctaatcg taccatagat caattctgta aataaaccca catctttttg
     7921 atttagctcg ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt
     7981 gttcactcgc tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg
     8041 gatttttttc attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg
     8101 aaatcatgta cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg
     8161 gtctgtttac cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt
     8221 gctttgtctg tagtttctgc caatacttca accgataata atttccagcg tatgccctca
     8281 taggtcgtga atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct
     8341 gcagttttat gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct
     8401 tctgactcgt cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc
     8461 aatagctttc ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta
     8521 gtgattggta tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc
     8581 atgatcgtta caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct
     8641 ccccgatatt taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc
     8701 aataactttt caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat
     8761 tcaatgattt cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata
     8821 ccgtgcttca gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact
     8881 ggacggtcgg gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca
     8941 agaatcggca cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc
     9001 ctccatatat tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa
     9061 gataccgtct aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt
     9121 cacttctatc tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt
     9181 gaccgttcca tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt
     9241 tgcttcgatg atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac
     9301 agcaattctc ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat
     9361 cattgtctca tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg
     9421 ctgagcagtt ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg
     9481 gccctttctt aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc
     9541 tgttctttct ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt
     9601 ttatatttga tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc
     9661 gttggcccta atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga
     9721 aagattttct ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag
     9781 taataaggag gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca
     9841 taattttgtt ttttagccaa ttctatggcg taatgttgag gattgaatgt ttggatcacg
     9901 acttcgcctg ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat
     9961 gtatgctcac tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca
    10021 actaatgtca cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata
    10081 tctgcttctt ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg
    10141 gtatctacat ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct
    10201 actttttgag tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga
    10261 ttgggaatag gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc
    10321 agtgtaagag agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc
    10381 acaaaagacg aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat
    10441 cgattttgta ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac
    10501 tcttcacgca tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg
    10561 tttaactgca ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc
    10621 gtcgcgcttc ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct
    10681 ctcgcatgat aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata
    10741 atgatcagcc cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact
    10801 tgcgcttctt cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg
    10861 ctatgtaaga ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga
    10921 gtcaatgcga tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg
    10981 attgcctgaa gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc
    11041 tgatcttttt gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca
    11101 ttcagttcca atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt
    11161 tctgattcta taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg
    11221 attcctttat ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa
    11281 agaagttctt tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc
    11341 ttttcagtta acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact
    11401 tcatatttga tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct
    11461 tcttcttctg cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct
    11521 ggtgtgtctg ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc
    11581 atcgtttgta gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat
    11641 gaaagtaatt cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa
    11701 gatggattag ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga
    11761 ttgccattac caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca
    11821 gagggaacca aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact
    11881 tcagctgtta atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa
    11941 aaagttcctt gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct
    12001 aacaaaaaat taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc
    12061 cttttctacc agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc
    12121 tttttagatg ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat
    12181 tccaattcgt gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa
    12241 cgttcacgtt ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct
    12301 aaagcttgac cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca
    12361 tccaattcat gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga
    12421 tctaatagtg agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt
    12481 aatatattta ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat
    12541 tcgttcaact gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat
    12601 ttcgatttct tcttttgcaa ctttcattcg tttttcgatt acttcatctg catctgttcc
    12661 tcggcctacg atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac
    12721 accatcgggc actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac
    12781 atcttttcct tcatccaatg tcttgttgac atatgacaaa ggagttccat aataattccc
    12841 gacatattct gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt
    12901 gcttcggaag aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat
    12961 tgagatcgaa tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc
    13021 ttttcctaca ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac
    13081 gtcctttcaa ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt
    13141 caaggttatc tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc
    13201 gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt
    13261 ttttcgtttg gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg
    13321 tcgtgaagaa gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac
    13381 atttttgcat aaatttggct taataccgag aaaatggttt ttgaacatca cttattcaaa
    13441 ataaaaaaag attgtgacac gaattttgtc acaaccttta aatgtattcc tagagcaaag
    13501 aagctaagcg aagaaatcca attctgttta tttattgtat ttctttgttc agcatccttg
    13561 ctaaaagccc atattggaca aagtattcac tacacccatg atctcatctt ttcgagaacg
    13621 tgctttttct gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa atttccgatt
    13681 catcgtcacg taagcaccgt cgttgatcaa aataaacagt tcgttctcat tatagtctgc
    13741 acaggcatct ttccatacgt caaagccgtt tttttcagaa gggatatccc gtttaaccac
    13801 agtttttcca cttaattcgt ggaacgttac gtcataatat ttcattatct ctacctcttt
    13861 cgtcattcaa tattttgtac ttgttctctg attttttcta agatcgtctt agactggacg
    13921 acttgatttt taatttcgat tgcatttgat ttggaaccga tcgtgttgat ttcccgattc
    13981 atttcttgga tcaggaaatc taattcccga cctacaggac attgtgcttc gataagttca
    14041 tctagttttt gtacatggat cgccagacga tccagttcct cgtgaatatc tccgcgttcc
    14101 aacaagatgg ctagctccgt caacaaccgt tgctcatcga ctttcgctcc aagaaattcc
    14161 tccagctttt tttgatacct tatttgaaat tccttttcat agatttctac aaaactatcg
    14221 agttttttta gtacttcttt gaattcttca gtattctttt tcaacactaa agctaaagct
    14281 tgaccttctt tttctcgact cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga
    14341 gctaactgtg ccaattcttc catgctctct tccggttttt cttctaccgt tacgaagttt
    14401 tcgtccagca cgaagcgttc cgccatccga ctaattgaca cttgattttg actaccgtat
    14461 cgttgttgaa ttccgtctgt caattgagtg ataagtgaat caatcaattc ccagttgatg
    14521 acgatctgtt tttcgttttt gccaatataa gctagattca caaatacttc gattcgaccg
    14581 cggcttaact gctgtttaat aagctgacga aaatcgtttt caacaaagtt caggatttta
    14641 ggacttcgta tttgaacatc tagaaaacgc tggttgacac tttttatttc aatatctaac
    14701 tgatacgctt ttgtttcgcg gatggctttg ccaaaaccag tcatactttt cattcgacag
    14761 ggtccttcct cattacatat tttctattgc tttttgcagt tgttcaaact cttcgttcgt
    14821 caatgtcagg cctttcccca ttttttcatg gtctggcgcc caatctcgaa gatcgaattt
    14881 tggcgggcga ccattccagc tgactaggtt cagttcttta cgccacccct tgttgttttc
    14941 tgacaatata gcgatttctt ctacgatttc atatgaaaat tcttgtgcca tggttgttcc
    15001 tccttgagat aatataaata taggatcagt aatgtgtcaa ctggttgctt aagcaattca
    15061 tgctgcaaac gatccttgcg ttttccttgt agcagataat caaaataaac agttttcgtg
    15121 cgctcatttt gtattttttg ctggtatttt cgataccttc gccattcttc agtacttaat
    15181 tgaccaattt gtgccaataa atcatccggc atacttttta tatcttgttg gcagatcatt
    15241 tgaatcatca tgtctacttg atagatttca ttgggcagta aaaaatgttt ttttgctaaa
    15301 agttctagca tcgccaatcc gcatgtctct ggtacgaccc tgttcttata taaattacgc
    15361 agtttttgcc aaaattttat gcttttgaaa aaagataatt ctaactgaat ttcttttgtt
    15421 aagtaagaca aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg aaatgtatac
    15481 cagttcccat agtaatagcc taatcgtttt ttcatttcca gatagcctaa ttgtaaattt
    15541 aattggttcc tttggctatc aaaaagcaga aagctgccta gtgtccacaa tgtttgacat
    15601 ttccaatgga taaaaatgtc tggcatacga atttttttcg ctgggcctgg cccttgcaca
    15661 tctacgacaa atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg tattttgttg
    15721 cgatacccac cgtctgcata tttatttttt ccgatctttc gataagccat agcgggataa
    15781 aatgaagcag aggcgagtat ccaatctggt atttcttctg tttttgtctg ttggatatca
    15841 gtcactactt ccgctagttc tggcagttta gtagacaccg tatataaacg tattttggga
    15901 ttatgtttga gtaaggacaa atccaaattg tctttgacca tcttttccaa tggagcagaa
    15961 gaaaccccgc cttcaattag tgctgttttc gtcatttggc gtctttctcg atagaagcgt
    16021 tcacgaaggt cttcaactgc agccatttct ggtaacgcaa gcacttgtct tgtcgacagc
    16081 tttttccata aattcacagc ttttttcata tctccttgta aaatcaatgc gccgttcaac
    16141 gcacctaccg aggtacctgt gataatctca aagttgatat catgttcctt cagcgcctgc
    16201 cacacaccaa tctgataagc accgtgcgct cctcctccac ctaaaacaag tgctgtatga
    16261 tagaccagct ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc
    16321 aaccattctt ctgatagagg agattgaaat aaaaaacaac agttttcttt aataaaaaaa
    16381 cgagcgcaag cttccatata ccgaaaaaat tttttccatt caaaagtttc accttcaatc
    16441 aacagattat taaaaataac atctcctcta cttttagcaa tagtaaaata gacggtatat
    16501 tttttcgata ctgctttaaa agcacattgc ttttttgcag aacgccaaaa agcgtctcta
    16561 ctttcttgat gactttgaaa aaatggaagg ggttgttttg cttgctggat aggcgaatac
    16621 agcgacattt tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc ctttttcttc
    16681 aagtatacca gtacgtcata aaaaggtcat gagtctcgta ttcgactttg tagattccat
    16741 aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata caaaaatcac aaaaattaat
    16801 tgaaaatgag agtgttttca atgaaaacgt gattttgtaa tggaatttta aaataaaaga
    16861 aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa ataagaggag
    16921 gaacatgatg actgagtcag tttatgtaca tattgatacc acaagtaatg ctgttttaac
    16981 aaaagggctg actgctactg actttgcaaa cagtatcgtg cattttcctc aaaacttgct
    17041 cttgcttgat ccatctgctt ctgctggaga atatgaatcc catacagggc tgaaagtaat
    17101 ccgtggtaca gaaaatattc agcgtttctt ttcctcttct cgtaacaggc gtggattcga
    17161 tgaattgaag tggatcgact ttactgattt aacgatgctg aaagaattga cacctttaga
    17221 aatttcagaa ttattatatt ttggtcatat gaaaacacat cttcattcgc cgttttttta
    17281 taaactacag aataattttg tatacttcga tcttaatgat cagctgaatc ggatttatta
    17341 tcgctattta gatgaatttt ataggatttt tgctagtaaa ttaagttcga tcttgtcaga
    17401 taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt
    17461 ggaattattg aaagaaatgc gtaatttgat gcaggaaggg atcatttttt cttttgatca
    17521 agttggtttg atcgatggta aatatcgtat cccaatctat gtgattgaag attatatttg
    17581 gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc gctactttaa tctactctgt
    17641 caccgatcga cactggcaat tggtagaaga tgccgatctt tcttatctgc cattgaagta
    17701 gatctatttc ttatcaaaaa ataaaaagga aacaagaaac taggacatga ttctgtctca
    17761 agtttcttgc ttctttttta tttacagctc ctgtcgttta tctggcacaa aaatcgaagg
    17821 agtcgtttgt tggatcccta ataaaacgat gatcgttaca atagctgtac tcaacccgct
    17881 ggcaccattc attacgaaag aaaagagcca aggattcatc ccccatagtg catacgctcc
    17941 ccagaaaacc actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc
    18001 accaaacacg atgctttttc taatttgttt cgtattatcg tctacatagc ctttttggac
    18061 atttgaagcc atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa
    18121 cacttgaacg acacttaaat aataaacttg tcccaacgga aaatgtagga tgccccaaat
    18181 gaaacctgcc agcatccctg cctttgtccc tcttcttaac gcaaaaagag tcaatggaat
    18241 ctgccctaat gaaatagaaa aactgctgcc gatctggatc ggaatcaacg acaacaccat
    18301 cgctaacgct gcaacaatcg ttccttctac ccaaactcta ttttttccca taaaaaaact
    18361 tcctcctttg aataaaaaag tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc
    18421 tgtttattca caattcctac gctcgcataa ccgatacagg tacgaagggt ttagaaatag
    18481 attcaatctc agcccaattg gactcccctt tgtgagttta tttattattt attttttgtt
    18541 tcaaacaatg gactaactgg tttgttttca tggatacgtt tgattgcatc tcccattagt
    18601 ccgccaacac ttacttcatc gattttgtcg attttacgat cttctggaag atagatcgag
    18661 tctgtaacaa ctagacgctc gatagctgaa tcttctattc tttgtaaagc agggccggat
    18721 aatactggat gtgtacatga agcatacaca ctagttgcac cagcttcttt caaagcattg
    18781 gcagccaatg tgatcgtacc tgctgtatcg atcatatcat cgatcagtac acaagttttg
    18841 cctttgactt gtccaatgat attcattact tctgcaacat ttgcttttgg acggcgtttg
    18901 tctatgatcg caatcggaga tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca
    18961 ccatggtctg gtgaaacaac gacaacatca tcgcctttga ttcctttttc taagaaataa
    19021 ttggcaataa gtggcgcacc catcaaatga tcgactggaa tatcaaagaa accttggatt
    19081 tggactgcat gaaggtccaa agtcagcatt cttgttgcac cagctttttg aatcatgttt
    19141 gctactaatt tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg acgcgcatag
    19201 ccataataag gcatgactac gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc
    19261 atgatcagca actccatcaa gttatcattg actgggctgc tcgttgattg aataacatag
    19321 acatgcgatc cacgaatact ttcttcaatg tttacttgaa tttctccatc actaaattga
    19381 gtaacagatg attttcctaa ctcgacgccg actgctgctg caattttctc tgctaatggg
    19441 cgattggaat ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt tgacatgggg
    19501 acctccactt tctttttcac aactctagaa ttctacctaa taaatagtag tcatattttt
    19561 taaataaatc aagtgatttt gcaaaagtct tagtttagat aagggagttt ttttgcatat
    19621 ccttctttat tgacttgtct tgctctcgca attgccagcg catattctgg aatattgtct
    19681 gtaattgttg acccggcagc aatcgaactg tttttagcta cctcgacagg tccgatgata
    19741 ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt gttctttcca
    19801 tcatagttga cgaagacaac accacatccg acattgattt cttctcccag cgtagcatca
    19861 ccaacataag tcaagtgacc gactttagtt cctttgccga tttgagcatt tttcacttcc
    19921 acaaagtttc cgatatggac gttttcgcca atttcagctt ttggtcgtaa atgtgcataa
    19981 ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg
    20041 acgtgatcct caatcctact gtctacgatt tttgaatgtg acccgatgac acaatcagaa
    20101 ccaatgactg tatttccttg gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt
    20161 tctacgcctg catcgatgta tgtcgttgct gaatcgacaa agcttacgcc attgaccata
    20221 tgcatctggt taattcgctt tctcatgatc tcatttgctt tagctaacgc gatccgatca
    20281 ttgactccca ttgattcttc aaaatcatcc gtttgataag cagcaactgt atggccttct
    20341 tcttttaaga tttcgatgat atctgttaga tagtattcac cttgagcatt gtttgttcca
    20401 actttgctta acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt
    20461 tcttgtacaa gagcttcttc tggtgttgca tctttttgtt ccacgatttt ttctacgatc
    20521 ccaatatggt cacggatgat tcgtccatat cctgttgggt tttctgcttg tgccgtcaag
    20581 atcgttgcac ttgcattttt tccttggtga tattcaaaaa gattgttcag cgtttcagta
    20641 gtcaataatg gtgtatcgcc actgataact aacgttgttc ctttttttcc ttccaagaaa
    20701 gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta
    20761 ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt gtccaacgat cgtaatcact
    20821 tcatctggtt tcgtttcact gacccgatta atgatatgtt ctaccatagg tttaccagaa
    20881 acgggatgta aaactttata taattttgac ttcatgcggg tacctttccc tgccgccaaa
    20941 ataattgcat aacgtgcgtc caactgcgtc actcctaaat atagtttgct gatttcagtt
    21001 tatcccagta tacccatttt ttcaacaaaa caagtgattt tcttttatcc cagcgttttc
    21061 tggataaaaa aatagataga ccataacggg cctatctatt tggtaaccgg tgctctcctg
    21121 ccaactcctc ggtcacaagt cacactactc ataaaacatg aaaagctgtt ttttgaaatc
    21181 gtttcttgtg acttagagtt aaacggtagg cttacaacct cttcattaaa cgtttttcaa
    21241 tcagctgacc ttcggtatta gctcaataaa cggcaaaata tgaggagcaa tttttccgtt
    21301 tattgagcta atactcagga caggacgctg atttcacaac ctctagataa acggtgttca
    21361 aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca
    21421 aaatttttct tactactcga aactaagcgg ctttttcaca acctcttatt ctgaaaaata
    21481 atttcctggc acaaccgtaa tatttttcgt ttgagtatct acatctttga cataaagcag
    21541 tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa atacagtgat
    21601 gccaacaagt tctgattcga attcttcaat caagctcttc attccgttga ctgttccgcc
    21661 gcctttcatg aaatcatcca ctacaaggac acgtgaacca cgtttcaaac ttctcttaga
    21721 cagttccatc ttttcaattc tttcagaaga tccagacaca tagttgacac ttactgttga
    21781 tccttctgtt atttttgagt cccggcgtac aataacgaat ggcgcattca gataatagga
    21841 cacagcttgg gcaataggta cgccttttgt ggctacagtc attacagcat cgattttttc
    21901 accaaggtat ttagaagcaa tgattcttcc aacttgacgc agcagttcag gttctcctaa
    21961 caaatcagag agataaacat atcctcctgg aagcagacgg tcttgttctg atagacgttc
    22021 tgctagagat tcaatatatt ttttcgcctc ttcatatgaa atttcaggga tgaacaatac
    22081 accacctgca gcacccggga ttgtttccaa ggttccatac cctcgttctt taaatgtttt
    22141 cttgatgatt cccaaatctt cactgataga agatttagca gattcataac gttcggcaaa
    22201 gcttgttaag gaaatcaatt catgcgggtg attcaataaa tactgtgtca tgtcaactag
    22261 acgttcactg cgacgtactt tcacctattc caactccttt taagaaattt tatcgtttta
    22321 cattatataa gtttttcttc aaaaattcga gcattttatg aaaaaataag aaaatagttc
    22381 ggtatttatt aaaataaaaa gaagcctata aaaaattttt cgtttttata aggcttcttt
    22441 tctttataat ttagttattt tttcttctga aggcaaccaa tattcgtttg atgatattta
    22501 caatcaggaa caaagcaata aagactaatg tgattgttgc tcctggaggt gtatctagat
    22561 aaaacgagct agttagtcct gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa
    22621 tcacgacatt gaagcttttg cctaatcgca taccgattgc tgcgggaaga accatgattg
    22681 cggatatcaa aagtgcaccg gcaatcggga tcatcacagc aatagccaca ccagtaatca
    22741 cattgaacat catcgacatc aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat
    22801 caaaagtcag aacatacatc ggccgtttga ataaaaagaa caatataccg atggcaacga
    22861 ataagattcc taaaaagatg acttgctccc atgtaatggt cacgatcgaa ccaaacagat
    22921 aggattggat acttgttgct gaattccctc cgctcaaatt catcaatact aacgctagcg
    22981 ccaaaccacc agccattaaa atggcaatgg agatttctga ataggttcgg tagatcgttc
    23041 gcagatactc caatatcaca gctgctaata caacgactag catggtcgta agtgtaggat
    23101 taaggttcaa aaagaatcct aacgctaccc ctgctaaaga gacatgagaa agcgtatcag
    23161 ccatcagcga ttgtcttctg atcactaaaa acacacctag catcggggcg attccggcaa
    23221 tgaagatagc ggcaagaaac gctcttctca tgaattcata tgaaagcaac gccattgaga
    23281 atcctccttt ctcaccagtc ggatctgtct gtccgcatac tgtttgatat cttcatgatc
    23341 atgggtgatc attagaactg atttcccatg gacatgcgca ctatgtcgga gaagacgata
    23401 aaattcattt ctggattctt catccatacc tgttgttggt tcatcaagaa caaataagtc
    23461 agggtcagta gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc cagacaattc
    23521 acctattcgc cgatttcgca tatcccacat ccctacagct tctaaagcac gctgtacatg
    23581 ttgatgatct ttttcagaga gacgcttgaa ccagcggcct cttggatatc ttcctgaacg
    23641 gactaattcg atcaccgtac taggaaaacc tgcattgaaa gatgcaactt gttgaggaat
    23701 ataaccgata cttaattttt ttccttcttg atttttctca gcaatcttta tcattccttt
    23761 tgaaggtttc agtaatccta acgatgcttt caccaaggtt gacttggctg ctccattttc
    23821 tccagttaaa atgacaaatt cacccgcatc tacatgataa gagacatctt ctagtacagg
    23881 ttcatcatca tagtaaaagg tcaaatcaga tatttcgata taacgcacct tacaacctcc
    23941 attctaaaac ttaattgata ctttctttta aagctttcag atttttttcc atgacagaaa
    24001 tataattttc gcctgctttg atttgttcat tagtcaagct ttctaaagga ttcaacactt
    24061 ctagcttcac tcctgtttca ttcgacaatg tctcagctac cttcgaagag gcattttctt
    24121 caaaataaat cactttgatg ttgtttttct tcacaaattc ttttagctca gctaagcgac
    24181 ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt
    24241 gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt
    24301 ttagctcatc ggagtatttt tgatccagtg cttcaagctt tttgatataa gcatcggcat
    24361 tcttcgtaaa agtttccttt ttatctggat atttttcgcc taattcttca gcaatcgttc
    24421 tgacttcttt tatagctaat accggatcga gccaaacatg tggatcgtat tcatgtgaat
    24481 gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga
    24541 ttcctttagc tgcttcgatc atcaaggtct gtttgctatc cactgaatcc tttaacgaat
    24601 ccacaaaaaa ttccatgtct gaactattat acacgaaaac atctgcatcg gaaatttctg
    24661 ctctttcttt tgcacttggt tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt
    24721 tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc atacatcgga taaaaagtcg
    24781 taacgatcgt caaatcctct tttttgtctg cttccttgtt cgtatttcca catgctgcaa
    24841 acagtaaagc acctatcatt accgtagctg tcaaaagata ttttttcatt tatatgctcc
    24901 tttcaatgaa caaatcggaa ttttacgatt tattaacgtg aactaatgta tcaaaataaa
    24961 aaaacacagt caatagaaat ccatcaattt ctgatttaag catgtatatt cttgattaaa
    25021 tcgtaaaatg gatgagttgc tgctcatatc tagccatttc actacaataa aaaactgaag
    25081 aataccattt taggattcct cagtttttca tcacctattt tacttcttat attttattgc
    25141 agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata tactctctgc
    25201 gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact catcaatgca
    25261 gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg atgccgcttt
    25321 cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata atcttgctct
    25381 cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt atctacttct
    25441 ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac ccagcactgt
    25501 ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc cgtacttccg
    25561 tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag ttcttctaac
    25621 gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca gtctgtactt
    25681 cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt gacaccggtt
    25741 ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt ccgttcatct
    25801 accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat ttcttcaaaa
    25861 aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg atacccatct
    25921 tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat
    25981 tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata gggaaaaata
    26041 aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca gctttcacag
    26101 aattttctct tcataatcca aacaagttct agtgattctt ataattctgt gagcacttct
    26161 tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag ctgcatggta
    26221 gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca atgatcgaca
    26281 tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc ataattatat
    26341 acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac attagctaat
    26401 ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat aaacagattg
    26461 gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa caaatcatat
    26521 cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt ttgatcttta
    26581 cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc cacagagaag
    26641 tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc ttgatgtaat
    26701 tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat
    26761 tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat ccctgcttca
    26821 cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct ttctgatatc
    26881 ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg attcgttgct
    26941 gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt gtttcctttg
    27001 atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa
    27061 cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa taaacggtca
    27121 aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa atattgataa
    27181 agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa ccacacatca
    27241 tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa gcgtccgctg
    27301 tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc atcaaagatt
    27361 atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga taaccacaca
    27421 gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg tgtgattgta
    27481 tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt gacttttact
    27541 gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac tgtctgatct
    27601 aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc agacagatta
    27661 gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact tacgagcacg
    27721 atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg attgattttg
    27781 actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac ataataaaac
    27841 aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa ttgactcatt
    27901 cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt cagcattcct
    27961 atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc tattctcatc
    28021 aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa ggatgccagt
    28081 agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct ctttagataa
    28141 gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat ccaatagctt
    28201 cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa cgaccataca
    28261 gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac catttttcta
    28321 tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc accaaaaccg
    28381 gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt tctttccatt
    28441 tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa caacatcgga
    28501 gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct ttgttgtccc
    28561 atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac ttgatttgcc
    28621 acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg ttcctttatc
    28681 cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact tgtatacttt
    28741 tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga cctgttcttt
    28801 gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata acatgaaagt
    28861 aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat taaaaaaagg
    28921 aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt
    28981 tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat cacaatcaga
    29041 tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag aactaagaga
    29101 atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa tagatttaga
    29161 aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag
    29221 cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg aacagcttca
    29281 gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag aatcagattt
    29341 cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc tgatcgatgc
    29401 gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg agattgctgc
    29461 aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc catgggaact
    29521 tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg ccttagaaga
    29581 agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga aagatcttga
    29641 gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc
    29701 tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa tcagttgcac
    29761 gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct tcttcgattc
    29821 gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct tctggcttga
    29881 ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc
    29941 tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat gattcacttc
    30001 gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca gagataacag
    30061 aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact gggttttctt
    30121 tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc tcatggtcaa
    30181 gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata tcttcgccaa
    30241 gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc aagacgtttt
    30301 gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct ggatgaagtg
    30361 tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt tcatccattt
    30421 ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt ccatcttgta
    30481 tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa
    30541 caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc
    30601 gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc ggaagggtaa
    30661 cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc gctaatgata
    30721 ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat tcttttacca
    30781 ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca tctcgtgcgt
    30841 cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca atcatttttt
    30901 caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct tcttctttac
    30961 tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc gtttgttcgc
    31021 gttgaatcaa acgattctct tgagatttta attctaactt actttctctc agctcacttt
    31081 caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct tcttttttta
    31141 gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat tgtgcaccag
    31201 ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga ccgacaatta
    31261 aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata ctatcttttt
    31321 catttttatc atctatacag cttctatgat aaattattct aaactcgtca atatgcatct
    31381 tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta ccccgtagtg
    31441 tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat ggtttttaaa
    31501 ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt ctattaaatt
    31561 attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa ttaaataact
    31621 cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc
    31681 agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt
    31741 ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga
    31801 aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg
    31861 accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc
    31921 tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac
    31981 ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt
    32041 accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt
    32101 tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc
    32161 tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc
    32221 agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc
    32281 catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat
    32341 atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc
    32401 aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc
    32461 taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat
    32521 agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg
    32581 tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat
    32641 tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct
    32701 cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat
    32761 aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat atctttttta
    32821 gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg
    32881 ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct
    32941 ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt
    33001 tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta
    33061 atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac
    33121 tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag
    33181 cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc
    33241 ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt
    33301 cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc
    33361 ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac
    33421 atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat
    33481 cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc
    33541 aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc
    33601 tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag
    33661 aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt
    33721 tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt
    33781 tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag
    33841 agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa
    33901 tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa
    33961 acgctgctga ttatctccta caactgtttg ataataaact tcgataccta gatcagctag
    34021 ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc
    34081 gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt
    34141 ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc
    34201 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
    34261 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
    34321 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
    34381 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
    34441 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
    34501 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
    34561 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
    34621 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
    34681 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
    34741 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
    34801 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaccctaca
    34861 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
    34921 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
    34981 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
    35041 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
    35101 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
    35161 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
    35221 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
    35281 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
    35341 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
    35401 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
    35461 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
    35521 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
    35581 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
    35641 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
    35701 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
    35761 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
    35821 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
    35881 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
    35941 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
    36001 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
    36061 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
    36121 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
    36181 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
    36241 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
    36301 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
    36361 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
    36421 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    36481 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
    36541 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
    36601 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
    36661 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
    36721 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
    36781 taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa
    36841 tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc
    36901 aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga
    36961 atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc
    37021 tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt
    37081 ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa
    37141 ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga
    37201 agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc
    37261 tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt
    37321 aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg
    37381 tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat
    37441 caatttctca ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa
    37501 cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc
    37561 cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat
    37621 ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt
    37681 gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt
    37741 cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc
    37801 attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa
    37861 caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc
    37921 atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt
    37981 gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata
    38041 aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa
    38101 agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc
    38161 tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca
    38221 aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca
    38281 gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta
    38341 attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga
    38401 cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt
    38461 tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga
    38521 ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca
    38581 gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt
    38641 gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg
    38701 cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg
    38761 ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga
    38821 ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg
    38881 cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt
    38941 tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata
    39001 aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac
    39061 cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata
    39121 atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat
    39181 aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt
    39241 gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta
    39301 agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac
    39361 agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc
    39421 ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt
    39481 atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta
    39541 ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa
    39601 gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt
    39661 ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata
    39721 ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt
    39781 gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt
    39841 acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag
    39901 aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat
    39961 cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta
    40021 gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga
    40081 ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt
    40141 cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact
    40201 cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt
    40261 tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt
    40321 cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt
    40381 aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt
    40441 catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga
    40501 cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta
    40561 agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg
    40621 aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc
    40681 aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc
    40741 aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc
    40801 tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc
    40861 taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta
    40921 taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat
    40981 aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt
    41041 ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa
    41101 acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc
    41161 tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat
    41221 tgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt
    41281 ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa
    41341 cagagaatag atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt
    41401 gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc
    41461 ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata
    41521 tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc
    41581 ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat
    41641 tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt
    41701 gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat
    41761 gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt
    41821 cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc
    41881 ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat
    41941 aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat
    42001 tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat
    42061 atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct
    42121 gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca
    42181 acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca
    42241 aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca
    42301 attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta
    42361 aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa
    42421 atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg
    42481 caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa
    42541 ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa
    42601 tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt
    42661 ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg
    42721 caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa
    42781 cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc
    42841 ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg
    42901 cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg
    42961 actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc
    43021 cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga
    43081 atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc
    43141 atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc
    43201 gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga
    43261 tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt
    43321 gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca
    43381 tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa
    43441 tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt
    43501 tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt
    43561 gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata
    43621 aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg
    43681 cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg
    43741 tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat
    43801 tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc
    43861 ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca
    43921 tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca
    43981 agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt
    44041 tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag
    44101 cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt
    44161 ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa
    44221 cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct
    44281 cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt
    44341 cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt
    44401 tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat
    44461 agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc
    44521 ttggatgcag tgtagcaggt tcgacattca acgctttcgt atcttcttca aatgctcgga
    44581 tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt
    44641 tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat
    44701 agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat
    44761 acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt
    44821 ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc
    44881 tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt
    44941 caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat
    45001 ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc
    45061 ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc
    45121 ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc
    45181 tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa
    45241 taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa
    45301 accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc
    45361 tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat
    45421 gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa
    45481 agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac
    45541 gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg
    45601 tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc
    45661 cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag
    45721 tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa
    45781 ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc
    45841 ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac
    45901 tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc
    45961 agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga
    46021 cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt
    46081 cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata
    46141 aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac
    46201 ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa
    46261 cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa
    46321 taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat
    46381 aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat
    46441 caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg
    46501 ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg
    46561 atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa
    46621 gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc
    46681 gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt
    46741 ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc
    46801 tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta
    46861 tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga
    46921 ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa
    46981 ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt
    47041 atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa
    47101 tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg
    47161 tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc
    47221 gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt
    47281 tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt
    47341 tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg
    47401 catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa
    47461 cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt
    47521 catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca
    47581 tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa
    47641 caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac
    47701 catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt
    47761 ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt aggatatcta
    47821 atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt
    47881 cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat
    47941 cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt
    48001 ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt
    48061 ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca
    48121 atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa
    48181 tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac
    48241 cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt
    48301 tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc
    48361 ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct
    48421 gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag
    48481 taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga
    48541 acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg
    48601 cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga
    48661 cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt
    48721 caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga
    48781 cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt
    48841 aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag
    48901 atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg
    48961 tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag
    49021 cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt
    49081 cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt
    49141 tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg
    49201 tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac
    49261 cgacgataaa aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt
    49321 ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga
    49381 tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca
    49441 tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta
    49501 attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg
    49561 ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa
    49621 tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt
    49681 ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt
    49741 tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga
    49801 ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc
    49861 cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta
    49921 tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga
    49981 accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa
    50041 aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt
    50101 ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat
    50161 cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc
    50221 aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg
    50281 tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa
    50341 cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa
    50401 atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata
    50461 tttagaaata acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc
    50521 ctattctaac atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg
    50581 ctatcttaaa gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa
    50641 aactattttt tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt
    50701 ggatatttga ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt
    50761 caaagtagga acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga
    50821 tcagttagct tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc
    50881 atcaggagct gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat
    50941 tcctgaacag caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca
    51001 aagattcagt gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga
    51061 ttatcctgaa agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac
    51121 gatccctatc gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa
    51181 atttggcgat aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt
    51241 gatcgtcctt tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc
    51301 tcagatgtat cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc
    51361 tggtagcaca tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt
    51421 tgaagcaaac agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat
    51481 tcttgcagga aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg
    51541 caattaggac aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc
    51601 aaaaatgagc tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt
    51661 gaagcaaatc ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa
    51721 gaccgcttgc agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt
    51781 gcttcgttag acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg
    51841 agaatcggca aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca
    51901 aatgtcacga cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc
    51961 ggtggcaaag aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg
    52021 gtcagtgctg gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact
    52081 gcaggacagt tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca
    52141 aaattaatcc aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga
    52201 aataatcacg tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc
    52261 aatgcaaaag cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct
    52321 gagatcgcac cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct
    52381 ttacgtgcgg atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca
    52441 gattgggata ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat
    52501 gaagcaattg cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat
    52561 agttatgcgg ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat
    52621 gcctccactc gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct
    52681 actcaaaagt tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat
    52741 atcatttatg gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc
    52801 ttttgccttt tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt
    52861 ttaatgattt tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt
    52921 attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg
    52981 tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac
    53041 atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc
    53101 gcttatacga ttacgctact gattgctggg atttttcact tatcttataa atatcagctg
    53161 aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc
    53221 cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca
    53281 gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct
    53341 tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc
    53401 atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc
    53461 cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc
    53521 gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat
    53581 tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat
    53641 tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt
    53701 cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa
    53761 taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga
    53821 aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt
    53881 ggtaaacggc gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag
    53941 aagctatttt tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc
    54001 tgttaaacgg tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga
    54061 ggaactattt ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc
    54121 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    54181 tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
    54241 ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac
    54301 atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt
    54361 caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat
    54421 agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca
    54481 gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg
    54541 gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta
    54601 aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa
    54661 tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa
    54721 tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga
    54781 ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta
    54841 cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa
    54901 acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac
    54961 cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt
    55021 tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag
    55081 cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc
    55141 cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac
    55201 cgatccgact agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac
    55261 tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta
    55321 agattgggtc tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta
    55381 gcgctgaact tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct
    55441 ccaatatttc catccctttc tctctattag catcatacat gatttatttt acatgtcaat
    55501 atgcacatgt aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt
    55561 aggattttta tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt
    55621 tctgggtatg aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc
    55681 tatacaacgc ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag
    55741 cactctcaaa aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg
    55801 atcaaagagg aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct
    55861 ttttcgacgc tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg
    55921 aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa
    55981 gaatttggac gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa
    56041 tggtgtcagc aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc
    56101 tttctagaaa tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa
    56161 aagaaaaagc gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga
    56221 actgcattca agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt
    56281 ccatcattca ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat
    56341 caactgcaac ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa
    56401 taattgatca ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat
    56461 tcctaaaacc atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac
    56521 tggtagttta aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa
    56581 agccaccacg aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg
    56641 cagccataaa ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa
    56701 aattattcct tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa
    56761 aatcatcgta gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta
    56821 tttcataaac ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa
    56881 tcgttcccgg atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa
    56941 aattagtcgg ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg
    57001 tcagacgggt cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag
    57061 caaaccctaa gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca
    57121 tgacaaaacg aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt
    57181 gaaagcttac tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata
    57241 aggttgtctt acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact
    57301 gaaaatcacc atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca
    57361 tgatgggata gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca
    57421 tctgctgcgg cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat
    57481 tcttttggat ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccagtc
    57541 tgatttgttg ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct
    57601 tttcttggat cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta
    57661 gctgagtctt tttctccaat tccttttgag tgagataggc actataattc cccgaaaact
    57721 ctcggatttc tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc
    57781 gatcatgact aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat
    57841 caatactttt tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag
    57901 aagaaagaag acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg
    57961 aagtttcttc cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt
    58021 cagcgagata ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat
    58081 gatcgggaaa aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt
    58141 ctcctatgat ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta
    58201 gctggttgcc aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt
    58261 ttacactcct atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct
    58321 gtttgcaaaa aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat
    58381 caccaaatct gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc
    58441 atccatgaaa ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt
    58501 cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac
    58561 acacgattca aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacat
    58621 agtgatcttt tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat
    58681 tgataaaata ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg
    58741 atctgcttcg aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat
    58801 gaattgcata gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga
    58861 aaaactcttg taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat
    58921 cttccctgat tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc
    58981 gccaaaaatc atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa
    59041 aggacgcaag atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggtttcat
    59101 attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg
    59161 gattttgtat gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa
    59221 atcatttttt tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt
    59281 gggtattcaa tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg
    59341 gggatagcac ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg
    59401 ctttttctgt tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa
    59461 catagccatt tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca
    59521 acttttcttg caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct
    59581 cgtatctagt aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg
    59641 ctaatttgat ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc
    59701 taataattga ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca
    59761 aagaatcagc tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt
    59821 ttttgtagaa tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg
    59881 attcttatca aactatagaa gatagctctt ctgcagtaga taattttaca ccgccaaaaa
    59941 tttccgaaat cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt
    60001 aaacagtaat aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt
    60061 tcaaaaactt gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt
    60121 tgtttggctg ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac
    60181 cataaaatcc tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag
    60241 cactcaaggt atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg
    60301 gcttggaacc accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag
    60361 agttgatctc agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac
    60421 ggtagacagg ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta
    60481 cagttggcag ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac
    60541 ggttatacag acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat
    60601 tataggtttc gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac
    60661 ctgttagcca agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat
    60721 agtagtaaac gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg
    60781 attctttgta cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca
    60841 catattccga agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata
    60901 gattgtaatt aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt
    60961 gagcaatcat gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg
    61021 caatactatc aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc
    61081 cagtatagtt ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat
    61141 ctgtcaattg gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg
    61201 tactgtcttc ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg
    61261 tattttctga gacagtagct gactgcaaag tactttctgt atcgttttca attcctgttt
    61321 ctgaagaact cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg
    61381 tgttggcaac tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca
    61441 tcttttttct cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa
    61501 aagcaagtca atgtccatat ttaaataaca tttgtttctt tttaataaat ttgctttttc
    61561 tgcataaaaa gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg
    61621 tttgaataac tatttaacaa gtaattctct ctggatatta tccgttatgg attatacttc
    61681 ctgtgtcgtc ggacggataa tcatgttgct gacagacatc cggtctggcg tttctatcgc
    61741 aaatgctaca gcagaagcta tatcttcaga tgttaatccc cattcttttt gcgcttcatg
    61801 aagttcaaga gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc
    61861 tggagagatg atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat
    61921 gattgcacgg acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg
    61981 tcccgccaca gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag
    62041 tacggcggaa attccattta acacacccat gatgttgata tcaagcatat tttgccattc
    62101 atcacggtga ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac
    62161 atcgattcga ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc
    62221 tcttacatct gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt
    62281 taggcgatct tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt
    62341 cgttgttgct tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa
    62401 atcattcatt ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt
    62461 atttcatctt actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc
    62521 tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc
    62581 attctcgcca atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt
    62641 cttgatgtgg attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt
    62701 caccgtgatg aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc
    62761 taagtcaacg aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt
    62821 gccgaaatct gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat
    62881 gatattttct agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc
    62941 taaaggctct tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac
    63001 tcgaatctct ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt
    63061 tatccttact ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta
    63121 ttccttttaa ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat
    63181 tcgtatactg acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa
    63241 atctctgcat atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact
    63301 tggtgtgaca cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata
    63361 tctttattca aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac
    63421 aaatgacgca atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc
    63481 tttcatttct gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt
    63541 atatagcggg aatgcagatg atatcataga caaactggat agcggattac ttgattttgg
    63601 actcgtaatc gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga
    63661 tcgttgggga cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc
    63721 gcaggaactg aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact
    63781 agctgaatgg ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt
    63841 gtacaatgct tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat
    63901 catcaatact tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc
    63961 actgaatctc atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga
    64021 tgtcctaacg gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac
    64081 aacgaataag ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc
    64141 ttccttttat ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa
    64201 acataactgt ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc
    64261 atactaggca tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat
    64321 ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt
    64381 gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga
    64441 caggaaaaag tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt
    64501 tttgatcgtg atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa
    64561 aatttactta atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat
    64621 gcggaaactg ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac
    64681 attgcttttt ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga
    64741 gaaatcgaag tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg
    64801 acaaaagagc ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt
    64861 cacagcggac gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta
    64921 tctgacatca tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt
    64981 tcactgaatt attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg
    65041 caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa
    65101 agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc
    65161 acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta
    65221 caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg
    65281 gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg
    65341 gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta
    65401 acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag
    65461 tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg
    65521 ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat
    65581 cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc
    65641 tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc
    65701 gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg
    65761 tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct
    65821 ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc
    65881 aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt
    65941 tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca
    66001 tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca
    66061 aagcaaaaac agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa
    66121 cagaaccact tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa
    66181 ttttcactgg gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct
    66241 ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat
    66301 tgatgttgat cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg
    66361 ctatcaaaaa attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg
    66421 atactgcaac gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg
    66481 cattaggcgg agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc
    66541 aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta
    66601 tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg
    66661 cagatgagat ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg
    66721 tggttaaaac agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa
    66781 atgggaaact gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg
    66841 gtgacggcta tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga
    66901 tcacaaacat tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag
    66961 tcttgcttca tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg
    67021 taaaagaagg tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta
    67081 ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc
    67141 aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt
    67201 ccaacaaagc ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct
    67261 gagacaaaaa tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact
    67321 ccgcggtaat aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc
    67381 ttattacttg atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag
    67441 tttatcggta tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    67501 cttcatactc aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa
    67561 tatttctttt aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc
    67621 atcagaggaa atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc
    67681 agctgcttct tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc
    67741 ttcaataatt tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata
    67801 gtcagttaga ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt
    67861 tgctgcagct tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc
    67921 ataaacgaat tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata
    67981 ccaattattc cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa
    68041 ttggtgatct ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc
    68101 gatataagat accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg
    68161 atttcctgga acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat
    68221 tgagtctaaa tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc
    68281 tcctttttct tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa
    68341 gaattccagc gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt
    68401 tgccactccc tattcttttt atttgttaag atttacagct gtacatttat aaattacacc
    68461 tttatcataa agagtgtggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga
    68521 tgaagtattc tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc
    68581 aagatacccg atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa
    68641 atgtacgtaa aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa
    68701 aaccgcagcc ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca
    68761 gcatcgaagg taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg
    68821 ttggtgcgaa tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag
    68881 aagaagaaat ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa
    68941 aagaaaaaag accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt
    69001 gcctgcattt ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa
    69061 gccttgtcat gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt
    69121 cttgataaac ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat
    69181 ggaaagctat ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc
    69241 tctatattat catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt
    69301 ggtaactccc atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc
    69361 tcttaaaaca tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc
    69421 tgctaaagcg attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt
    69481 tggaacatcc tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc
    69541 gttgactgta aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt
    69601 accgataact ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt
    69661 gctactcttc aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct
    69721 atactttgcc atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa
    69781 gggaaatgac acgattacgc tcttttttct agggatattt ttatttttta actatttttt
    69841 tgctttcact gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt
    69901 aaaagagatc tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata
    69961 gattttcccc caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc
    70021 ccaagtgtgt tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt
    70081 agcaaagcca atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc
    70141 ataagaaacg ttgattactt gatttggata taaatctcct aacttatgca tctcttccct
    70201 ttgagtctcc gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt
    70261 gacaggaact tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa
    70321 ttccatcgat tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg
    70381 tatgccgtat ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga
    70441 aaaaccttcg actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc
    70501 taggagatcg cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat
    70561 ccctgcaata aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc
    70621 tattatttta taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt
    70681 cagatcttta agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa
    70741 agtcaattac ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat
    70801 ctaaagaaaa ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat
    70861 gttttacctt tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa
    70921 aaatatcttt tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg
    70981 ttcaaactgc tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt
    71041 atcaattgga acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa
    71101 aaacaaaaaa agttccatct ttaaaacacg ctattctttt actttttctc acattctcta
    71161 agaaaagaaa aggagaatga atttttgaca attcgagcaa aagtgattat caaatatgta
    71221 aaatcagcta cttttttctt aaacgtttct ttatactaaa tataaataag tgtttcatga
    71281 aaggagaact agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt
    71341 tacagctatc atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc
    71401 gcaacagttt ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac
    71461 atgacttgtg attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc
    71521 ttattcgctt acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg
    71581 tcccttgttt cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata
    71641 gaacatccgc aatttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat
    71701 tcagataaat taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac
    71761 aggaaaacag cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg
    71821 ctcattcagc aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact
    71881 ctcgaaacat tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt
    71941 tattatttat ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc
    72001 tcttggttcg tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactccata
    72061 aaaatcctga aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat
    72121 aaaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca agtaaacgat
    72181 aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac acgaatcatg
    72241 caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg actattaggt
    72301 acgcgttttg ttttataagt catgacaaat ttccttcgtt gctgggtaga ttttccggca
    72361 ttgttgatag attttatgat acttttctac attttgtaag agaggttcca ctgcatctgt
    72421 atattggata aaattctgac aacaactttc aggtgtgtcg aaccactcta atctttaaag
    72481 ataaatatta aaataacgat taataatatt aacagtcttt atttttcacc accaaaaacc
    72541 tatctttgca tatactaaat agacgtatat acacagatag gtttgtcttg tgaaaaataa
    72601 tgattatcct taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc aaaaaatatg
    72661 atccttattg aaattactgg ctatttatat tccaacgact tcttgtgtta atttttgtta
    72721 tctcatagta aattatgttt cattttcact taatttagtt gtcacgtgtt cgtcaattcg
    72781 taaaaacggt aaatatataa acacacttgc ggctataatt aatatttgta aaacagctgc
    72841 tcgccagtct ccggctgttg ctaaaaatcc cgatataact ggtggcgtag tccaaggaat
    72901 ttgcactact acatgattaa ttaatcccac agcagttgca aaatatgcta ctgtcaaaga
    72961 aaaaatcggt gcaagcacaa agggaataat cagactgata ttaaatacaa taggcaatcc
    73021 aaaaataata ggttcgctta tattgaatat agcgggagtt accgataatt tagcaaagtc
    73081 cttataatct tttctacgac tgaagataaa tattgcaatc aataaagcaa tcgtatttcc
    73141 cgatccaccc attaccgcaa aggtatcttt aaaggccata ttgataatat gtggaatttg
    73201 ctctttgttt gcataagctg ccatattttc ttgcatattc tgaatcaaaa acggatctaa
    73261 caatgaacct gatattactg cttgatggat accaaatcca aaaaatagat tagcaataga
    73321 tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta cttaatggct ctgtaactat
    73381 cgtggtaatt agcttattta tatccatatt aaacaattga atgattataa atgacaataa
    73441 agcaaacgag gtaatcgtaa taatgattgg gattaataca ttaaaagact ttaaaacagc
    73501 gggaggaata tttccggata gctgaatctg tagtttctta ttattagaaa gtttaataaa
    73561 taattctgta gccgctaatc caacaaatat acctacaaac attcctgaag ctcctgtata
    73621 ggatataggt atcacacccg tcacttcaac agctttatcc gcattttcag gagtaaacat
    73681 catctgcata ggtgttaata caacaaatgt cgaaatagca actaataccg ctgcaatatg
    73741 atttgtataa tttctgcttg cacacatatg ataagatact gctgctacaa ctaataatgt
    73801 agttatgctt aatgtaccat tggcaattga agtaccaatt tgttgtaacg tggtcaatgt
    73861 actagtatcg acaatttccc ccatgaatcc tgttggttct aagatcactg aattaataag
    73921 tgtcacaaat cctgctaaca tcataaatgg cataaatgta gcaaacgcat ctcgcaaagt
    73981 tcttaaatgg atctgattcc ccaacttagt cgcaactata ccaaattttt caaatacaat
    74041 gtccttcctg ctacttttgc ccatattttt tctcctttat gcttattgta atcgattact
    74101 ttttccaccc aaataataca tcacttatca aaaaaagtaa atacttttaa ttaaatatat
    74161 atatcttttt tagtataaaa aaataagctc tgaaaaaata attttcccga gcttattttt
    74221 caacaaaaac agcttcaaaa acaaagtctt gatataaatg tctcgatacc gaatactcga
    74281 aaggtttacc agacgataga gcaacaactt tgtctatctc aatcataaat tcacctggat
    74341 ctatttttaa tatttttgcg tcaaattcat tcgcagtatc tcctttgata cgtatgtgcg
    74401 aagtctttat ttccagaccc agttcatttg tgatataagc atatacagat ttctttaaat
    74461 gttttggttc caagcctggt atgactgcta atggcatgaa tgtttgctca atcgagtatg
    74521 gttgacaatc aataaaacgg cttcgtacaa tattatatac atagtctttt acactgatac
    74581 ttaatttatc agctactttt ttcgatggaa gttcaattga gaaagtatgg atttctgaat
    74641 ctacgtgttc ttcaccaacc acgctaaaca aaccttcgtt tggaccaaga actcgtgagt
    74701 tatcaattgg tgttctgacg acttcagtcc catacccact tctacttcgc aaaactcctt
    74761 cggaaacaag taagtctacg cctttcttta ctgttaactt gcttgtatga taaataactg
    74821 ccaattcatc tcctgtagga agtttctcac caattttata ctctccattt acaatttttt
    74881 tctttatatc taagtaaatt tccatatact tcgcaatttt tttcaatggt gaccctccaa
    74941 tcaaaaacat atcttcatct atactttaac ataaataaac cgataaaatc cacatcaaaa
    75001 ttatacacac aataaaaaag atatatatat ttaaacaaaa ctatttactt taagttgtaa
    75061 gcggtgttat cattaagtgg aaacaggagg taaaaaaatt atgacaacta tgaaaaagct
    75121 aacttttcca gatgattttt ggtggggttc cgcttggtca gctgagcaag cagaaggaaa
    75181 aggagaaaca ggaaaagcag agactatctg ggatcgctgg tttaaagaac aaccttaccg
    75241 gttttatcaa cgaatcgatt cctctattac aaccgatcac atccatcaat ataaagaaga
    75301 tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt cttgggcacg
    75361 aatgtttcca aatggcggta ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa
    75421 attattttcc gaaatgaata ataacggaat caaagtattt gccaacttat atcattttga
    75481 catgcctgct actcttcaag ataaaggagg atgggaatca cgtgaagtag ttgatgctta
    75541 tgttcattac gctgaaactt gttttaaaga atttggtgac ttagtttgtc attggtttac
    75601 cttcaatgaa ccacttgggc ctattttagg tacttatctt gaagattttc attatccaaa
    75661 cctcattgac tttaaacgcg gagcgcaagc agcattcttc actatacttg cacacgcaaa
    75721 agcaatacaa gtatttaaag aactagctct tcctagtaaa ataggcgtta ttctaaattt
    75781 gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga
    75841 tttattttat acgcggagct ttttagatcc tatggttaaa gggaattttt ctaaaaagct
    75901 tatcctgtta ttacaagaat ataatcagct tcctgaagat tatacgaaag aagatcttgt
    75961 atgtatctac gaaaataccg ctcaaattct tggactaaac tattacgagc cacgtcgcgt
    76021 caaatctcgt tcttctatgg tggatacatc tagtccattt ttaccagagt ggtttttcga
    76081 accacatatc atgcctggac gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa
    76141 aggaatttat gatttgtgca tggatatcaa gaaccattat ggaaacatcg aagcatttat
    76201 ctcggagaac ggaatgggcg ttgcggatga agaacgtttc atggatgata cagggcaaat
    76261 aattgatgat tatcggattg agtatatcaa agatcatctt gcttacttat ggaaggcaat
    76321 aaatgaagga tgtaacatta aggggtatca tctttggaca tttattgatt gctggtcatg
    76381 gatcaacgcc tataaaaacc gatatgggct ggtttcactc gaccttacta ctcaaaaaag
    76441 aacaattaaa aaaagtggcg aattttacaa gcaattaagt gatacaaatg gatttagata
    76501 cgatgtagac ttgttagttg aataatattc taaaattcca cttgaagtga acacattaga
    76561 aaacaagaat aatgagaaat accaataaca aatacttttt cttcatactt catttatact
    76621 tgtcagtata agagactgtg acaaatgtag tgatcctcag aagttaggtc tggaaatcta
    76681 acttgagaag atttttttaa actacgtcca gtctttttta tttgtttaaa attgaagttg
    76741 acaagtcttc attaaaaatt tagaatattc acaagtttga cgacaattat gaaaatttat
    76801 gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag
    76861 acacgttacc taccgttttt ggttatatcg gtattggact tgcatttggt atcgttggga
    76921 aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt
    76981 ctgcccaatt tatcacggtc agcatgcttg ctagccatag tccactgctc tccatcgttc
    77041 tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt
    77101 tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt
    77161 tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga
    77221 atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt
    77281 tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt
    77341 ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt
    77401 tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagta
    77461 acttagtgat tttagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg
    77521 ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct
    77581 aggatcctac catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat
    77641 ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa
    77701 aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt
    77761 ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggtaggaat tctttccctt
    77821 tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta ctgtcatgcg
    77881 ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct tatccgttga
    77941 acggaccact aacaacgact tttcctaata tatgtcctgt ttctacgtct ttggttgctt
    78001 gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta ctgcggataa
    78061 tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat
    78121 agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat tggtttttcc
    78181 aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc
    78241 ggacaaggtg tttgatttga tcaaagtagc gggtaatatc gaataataca gcgacatact
    78301 ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga taatcgattg
    78361 gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca gtcgcgtaga
    78421 cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc ccagctccgt
    78481 tgatcactaa gatttttttc ccgcggttgg cattttcttc tggagtcaga ccaaattttt
    78541 caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg
    78601 aaagattttt tggtgcaaga gcgacaatac gttcgtcaac cagttggtat tcttgattgc
    78661 ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca
    78721 cttcatcacc tacagcaaca actttcccga ctccatcaaa ccctaagata cgtaaagcat
    78781 ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat actgcgttga
    78841 cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca tcaataaagc
    78901 tttttggatc atcgatcgac aacccttcat aaaaaccgac taccttcatt aattcctttt
    78961 ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag ttttcatttc
    79021 aatatatata gtatgctatt tgttccactt ttattcatca taattattct cttacttaaa
    79081 gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt tgtcacaacc
    79141 tcttttaaac ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac gacaaaatat
    79201 gaggagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacgacc
    79261 tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt
    79321 ttcaaaggtg tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac
    79381 tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc
    79441 tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag
    79501 tagtccgatc agttcttttt ctgtatgttc cctcggttct ttttcaaatg cataaggatt
    79561 cttgaaaatc ccccggccaa tcatgatgcc atctacgcca tatttttcgg ccaattcttc
    79621 tcctgtctgg cgatctggga tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg
    79681 gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt gcatctactt tactcatctc
    79741 tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat
    79801 ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa
    79861 accgccagct ttagccgctt caatcaattg tgccgctact tctgggcgaa ggatcaagcc
    79921 actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc
    79981 ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc
    80041 ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact
    80101 gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc
    80161 cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg
    80221 tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt
    80281 actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact
    80341 aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa
    80401 agactgtgac aaaaatctta tcacaattcc ttttccggaa taaatggtgt tcaaaagctt
    80461 gcatctgcgg taataagtcc aaccaaacga aaaataggag gagctatttt tgtttggctg
    80521 ttcttattac ttgatgcaga acagcttttt cacaacctct ccactaaacg gtgttcaatc
    80581 agctgaccct cggtattagc tcaacaaacg acaaaatatg aggaactatt ttcccgtttg
    80641 tttctctaat actcaggtca aaacgctgat ttcacaacct cttttctaac ttctcaatcc
    80701 tcttccttta ttgatcaaat accaagtaac ggcgtacaaa acaacgatga atagcacaag
    80761 tacgatcatc gatgttgcta ctggcacatc gattgttcca aggaatccgt aacggaaacc
    80821 gaagatcata taaacgatcg gattgacttt cgaaacagct tgccaaaatg gcggcaacat
    80881 agagatggca tagaaaacgc cacctagata agttaacggc tgcaagacaa atgtaggcac
    80941 gatcgataca tcgtcataag actgagcaaa aatcccgttg agcagtcctg ctaacgaaaa
    81001 caagattgcc gtcatcaata atgtaatgat gaccatgaac catgaataca catgaagtgg
    81061 tacaaagaac aaggaaatga tcgttacgag agagccgacg agaatactgc gacccactcc
    81121 gccaatcaca aagccccaaa tgatcacatg cgtaggaaca ggcgctacca agatttcttc
    81181 gatgtttttc tggaattttt gagagaagaa ggacgaagaa acgttggcat acgaactagt
    81241 gatagcagac atcataatca aaccaggcac gatgaattcc atatatgaaa aaccacccat
    81301 gtcaccgatc cgaccaccga tcatttttcc gaaaataaca aagtacaagg atgtcgtgat
    81361 cacaggcgga accagtgttt ggacccagat acgcaaatag cggttcgttt ccttgatagc
    81421 taaacttttt aatgcagtaa aataaagatt aaacattttt ctcctccgta tgattatttt
    81481 cttctgtgat tttcaaaaac agttcttcta aacgattcga cttattcctc atagaaagaa
    81541 ctttgattcc ttgtcttgtc agctgatcga agattccgtt caccccttga tttcgttcaa
    81601 cttcgacagc gattgtctgt tcatcttcaa atgtacaagc gtatccttca atcacaggtt
    81661 tggtctcata tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttg gatagaagct
    81721 ctttcatact agtattctcg atcagttcac ctgattggat gatcccgatg ttgcgacaaa
    81781 gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca
    81841 attctcggag aaactcccac atctcgcgtc tcaattcaat atcgactcct gctgttggtt
    81901 catccaagat cagcaaacgt ggttcatgca tcaatgccct agcaatcatc agtcttcgct
    81961 tcattccccc ggaaagcata cgtgcacgga catttctctt ttcccaaaga ttggactgct
    82021 tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt
    82081 gattcacgac gatttgctgg accgtttcaa agggattgaa attgaactct tgcgggacta
    82141 atccaatctg ttgctttgct cggactagat ccgtatccag atcataaccg aagacattca
    82201 cttttccaga tgttttattg actaaagaag taatgattcc gatcgttgta gacttcccag
    82261 ctccatttgg tcctaatagc gcatagaaat ctccttcttt cacggaaaga tcgattcctc
    82321 gcaatgcttc gaccccactt ccataaacct ttttcaaatt atttatttca agcgcatttg
    82381 ccatttattt cctatctcct tatagacatg attctttatt gtatcatgga atacagcaga
    82441 tgaaaaaaca acttggttcc agcgaaaaat caggttgctg ggctcaggaa aatctcctaa
    82501 tgaaagacat caatcggacg aagtcagaca accttttcag aaaaaacaaa tcgaagataa
    82561 aggattacta ttcatatgtg caacaaaagg agcgtgagac aaaagtaaag aacacttttg
    82621 tctcacgctc taaacacgta taaacggcgg aagcaaaagc caagattttt gatccaataa
    82681 atactttcga tcacttcgcc tatcccaata catgccactg ccgcttttca tcaaataacg
    82741 cggcttccgt ttttgcggtt tctactaaag tctctgggta atccaaaact ttcagcaagg
    82801 caatcgcatt tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt
    82861 ttccttctgt tacttgttct tcaaagtgga cattctcaca ataattcttt aaaatctctg
    82921 tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg atactccgca agccaccgaa
    82981 cgactgaagc ggaagaagca atccgttcga tcgtatttgt cccttttaaa atctcatcta
    83041 cgaagcagta gcagcgttct ttggaagcca cttggtccaa caaacgtttg atcgatttga
    83101 tttctgaaac aaaataactg tcgccttcaa ataaatcatc ttcgattgcc atggaagtca
    83161 acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta
    83221 gaatacagct gatggctata cttttgacat acgtagattt tcctgatgca ttggaacccg
    83281 tgactaatgt attcttctgc cagttgaccg gattcactac cgcttctttt aacaatggat
    83341 gatagatatc tttcgctgtc acaccgccct cagtaaactc tggtcggcaa gtgatgggca
    83401 tataagtacg gtaattcaat attgcagcgg ctacttctaa ttctcccagc aattcccaca
    83461 gcttgatggc ttcagttgaa tgcttttcta atttagtgag aacgaaatga taggagataa
    83521 atggcagcat gaacatgatg ttgacatatt caaaaatcaa ctctgcttca ctcccactct
    83581 tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa tggtttagtt tgctgtttca
    83641 gctcttcttg aagtggtgtc ggcagttttg ctagctgatt ggcggctgaa atcgttgaaa
    83701 ccaaataact catgctattc agctctgttt ctagtgtttg tttcttcact agataataga
    83761 ccgtattgaa tactaaccca ccgattgcca aatagattcc tagtacttgt cctaaaagaa
    83821 gcaaacccaa acccaccagc ggaagcaagc caagaaaaat aaataaacct acgcgtccaa
    83881 tctttttact ttttccatcc gctagatatt gttttgcaaa attatgatct ttttttccta
    83941 aacgagcgaa ttgatattgg attttttctc tcagttgagg attttcctga taaaaagcaa
    84001 tcagtttttc taactgctga tcttcaccaa aatcaaagct gcgtaaacgt tgatacaacg
    84061 cttctgaacc tacactagaa taggtactgt tgattccttc gaatacttcg aacatatcca
    84121 ggtcatacca agtaagatca tcgatttcgc tatcccatga acggaacttc ttctccgtga
    84181 gccatgcttc cttcaagctt tcttctttat caaatctcgg ttgataagga attttttccc
    84241 atttgctacg aactgtttcc tttaatttca aacgtgaaaa aatgtctaaa gcaactaata
    84301 aaataacagc tgcaataata attaaaacga taaattctct ttgacccata tccctctccg
    84361 ttcctttatt tgttagcttt agtatagcac tgttgcgcta tttgaaaaga gggtaaaaaa
    84421 acacctttca aaataaaagg tgtttttttc ttgtatatta catatttatc tctcatttat
    84481 caaaattgac aaataatcat agggaggaat tgttgttaat tcttgtttat tctccagtgc
    84541 attgacaact gtcccaaggg aaaggatagc ctcaggtact attttttctt gttctgttgg
    84601 attagctaag cgaagaacaa atccttgttc atgaaaataa gaaggataaa tcgatgatac
    84661 aattaagtct tcaggtaatt caatcaatga aaatttacga ggaacatcaa gttcattttg
    84721 taatggccaa atcttgttat ctaacctgtg aataaataca ttcagtttct gcttttgata
    84781 agaaatagat tgttctaacc gtttttctgc ctgttttgcc actttgaact cattaaatct
    84841 tccttcttct acattaaatc caaaagtgac tttatactca cctatctctt gagctagcgg
    84901 tgtttccatc atgacatgcc cttcatttgt cgtatctcct gaagctcgcc caggtcgcca
    84961 agcaagatta g
//
LOCUS       DANJMA010000006        84114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000006 DANJMA010000000
VERSION     DANJMA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            220..1278
                     /locus_tag="PWU31_000490"
     CDS             220..1278
                     /locus_tag="PWU31_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL1083060.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1282..2316
                     /locus_tag="PWU31_000491"
     CDS             1282..2316
                     /locus_tag="PWU31_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL1083061.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2418..3134)
                     /locus_tag="PWU31_000492"
     CDS             complement(2418..3134)
                     /locus_tag="PWU31_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1083062.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3103..3888)
                     /locus_tag="PWU31_000493"
     CDS             complement(3103..3888)
                     /locus_tag="PWU31_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL1083063.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3879..4628)
                     /locus_tag="PWU31_000494"
     CDS             complement(3879..4628)
                     /locus_tag="PWU31_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1083064.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4926..5450
                     /locus_tag="PWU31_000495"
     CDS             4926..5450
                     /locus_tag="PWU31_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL1083065.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5466..6965
                     /locus_tag="PWU31_000496"
     CDS             5466..6965
                     /locus_tag="PWU31_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL1083066.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7053..7655)
                     /locus_tag="PWU31_000497"
     CDS             complement(7053..7655)
                     /locus_tag="PWU31_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1083067.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7759..8715
                     /locus_tag="PWU31_000498"
     CDS             7759..8715
                     /locus_tag="PWU31_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL1083068.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(9020..9976)
                     /locus_tag="PWU31_000499"
     CDS             complement(9020..9976)
                     /locus_tag="PWU31_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1083069.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10150..10710
                     /locus_tag="PWU31_000500"
     CDS             10150..10710
                     /locus_tag="PWU31_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083070.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10703..11527
                     /locus_tag="PWU31_000501"
     CDS             10703..11527
                     /locus_tag="PWU31_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL1083071.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11537..12154
                     /locus_tag="PWU31_000502"
     CDS             11537..12154
                     /locus_tag="PWU31_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1083072.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12172..12855
                     /locus_tag="PWU31_000503"
     CDS             12172..12855
                     /locus_tag="PWU31_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL1083073.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13538..14821
                     /locus_tag="PWU31_000504"
     CDS             13538..14821
                     /locus_tag="PWU31_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1083074.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVNKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14837..15724
                     /locus_tag="PWU31_000505"
     CDS             14837..15724
                     /locus_tag="PWU31_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083075.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15741..16577
                     /locus_tag="PWU31_000506"
     CDS             15741..16577
                     /locus_tag="PWU31_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1083076.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16580..17203
                     /locus_tag="PWU31_000507"
     CDS             16580..17203
                     /locus_tag="PWU31_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083077.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17200..18492
                     /locus_tag="PWU31_000508"
     CDS             17200..18492
                     /locus_tag="PWU31_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1083078.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18851..18948
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19064..19645
                     /locus_tag="PWU31_000509"
     CDS             19064..19645
                     /locus_tag="PWU31_000509"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL1083079.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19648..20955
                     /locus_tag="PWU31_000510"
     CDS             19648..20955
                     /locus_tag="PWU31_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL1083080.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMILISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21371..21859
                     /gene="purE"
                     /locus_tag="PWU31_000511"
     CDS             21371..21859
                     /gene="purE"
                     /locus_tag="PWU31_000511"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL1083081.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21852..22976
                     /gene="purK"
                     /locus_tag="PWU31_000512"
     CDS             21852..22976
                     /gene="purK"
                     /locus_tag="PWU31_000512"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL1083082.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22989..24284
                     /gene="purB"
                     /locus_tag="PWU31_000513"
     CDS             22989..24284
                     /gene="purB"
                     /locus_tag="PWU31_000513"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL1083083.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24464..24841)
                     /locus_tag="PWU31_000514"
     CDS             complement(24464..24841)
                     /locus_tag="PWU31_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083084.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            25097..25825
                     /locus_tag="PWU31_000515"
     CDS             25097..25825
                     /locus_tag="PWU31_000515"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL1083085.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25825..26079
                     /gene="purS"
                     /locus_tag="PWU31_000516"
     CDS             25825..26079
                     /gene="purS"
                     /locus_tag="PWU31_000516"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL1083086.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            26081..26752
                     /gene="purQ"
                     /locus_tag="PWU31_000517"
     CDS             26081..26752
                     /gene="purQ"
                     /locus_tag="PWU31_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL1083087.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26753..28975
                     /gene="purL"
                     /locus_tag="PWU31_000518"
     CDS             26753..28975
                     /gene="purL"
                     /locus_tag="PWU31_000518"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL1083088.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28960..30399
                     /locus_tag="PWU31_000519"
     CDS             28960..30399
                     /locus_tag="PWU31_000519"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL1083089.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30422..31474
                     /locus_tag="PWU31_000520"
     CDS             30422..31474
                     /locus_tag="PWU31_000520"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL1083090.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31471..32049
                     /gene="purN"
                     /locus_tag="PWU31_000521"
     CDS             31471..32049
                     /gene="purN"
                     /locus_tag="PWU31_000521"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL1083091.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            32046..33581
                     /gene="purH"
                     /locus_tag="PWU31_000522"
     CDS             32046..33581
                     /gene="purH"
                     /locus_tag="PWU31_000522"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL1083092.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33597..34871
                     /gene="purD"
                     /locus_tag="PWU31_000523"
     CDS             33597..34871
                     /gene="purD"
                     /locus_tag="PWU31_000523"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL1083093.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34993..35907
                     /locus_tag="PWU31_000524"
     CDS             34993..35907
                     /locus_tag="PWU31_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1083094.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(36040..37605)
                     /locus_tag="PWU31_000525"
     CDS             complement(36040..37605)
                     /locus_tag="PWU31_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1083095.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSTVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37598..39049)
                     /locus_tag="PWU31_000526"
     CDS             complement(37598..39049)
                     /locus_tag="PWU31_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1083096.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(39063..40079)
                     /locus_tag="PWU31_000527"
     CDS             complement(39063..40079)
                     /locus_tag="PWU31_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1083097.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40299..41387
                     /locus_tag="PWU31_000528"
     CDS             40299..41387
                     /locus_tag="PWU31_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL1083098.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41441..42967
                     /locus_tag="PWU31_000529"
     CDS             41441..42967
                     /locus_tag="PWU31_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1083099.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42969..44174
                     /locus_tag="PWU31_000530"
     CDS             42969..44174
                     /locus_tag="PWU31_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083100.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44431..45408
                     /gene="guaC"
                     /locus_tag="PWU31_000531"
     CDS             44431..45408
                     /gene="guaC"
                     /locus_tag="PWU31_000531"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL1083101.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45672..47096
                     /locus_tag="PWU31_000532"
     CDS             45672..47096
                     /locus_tag="PWU31_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1083102.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47557..48666
                     /gene="pdhA"
                     /locus_tag="PWU31_000533"
     CDS             47557..48666
                     /gene="pdhA"
                     /locus_tag="PWU31_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL1083103.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48669..49646
                     /locus_tag="PWU31_000534"
     CDS             48669..49646
                     /locus_tag="PWU31_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL1083104.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49671..51314
                     /locus_tag="PWU31_000535"
     CDS             49671..51314
                     /locus_tag="PWU31_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL1083105.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51322..52728
                     /gene="lpdA"
                     /locus_tag="PWU31_000536"
     CDS             51322..52728
                     /gene="lpdA"
                     /locus_tag="PWU31_000536"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL1083106.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52890..53165
                     /locus_tag="PWU31_000537"
     CDS             52890..53165
                     /locus_tag="PWU31_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL1083107.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            53171..53938
                     /locus_tag="PWU31_000538"
     CDS             53171..53938
                     /locus_tag="PWU31_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL1083108.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            54106..55935
                     /gene="typA"
                     /locus_tag="PWU31_000539"
     CDS             54106..55935
                     /gene="typA"
                     /locus_tag="PWU31_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL1083109.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56196..57968
                     /gene="recQ"
                     /locus_tag="PWU31_000540"
     CDS             56196..57968
                     /gene="recQ"
                     /locus_tag="PWU31_000540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL1083110.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            58082..58384
                     /locus_tag="PWU31_000541"
     CDS             58082..58384
                     /locus_tag="PWU31_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL1083111.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58387..59550
                     /locus_tag="PWU31_000542"
     CDS             58387..59550
                     /locus_tag="PWU31_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1083112.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKNFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59576..63004
                     /locus_tag="PWU31_000543"
     CDS             59576..63004
                     /locus_tag="PWU31_000543"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL1083113.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            63025..64149
                     /locus_tag="PWU31_000544"
     CDS             63025..64149
                     /locus_tag="PWU31_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083114.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            64139..64576
                     /locus_tag="PWU31_000545"
     CDS             64139..64576
                     /locus_tag="PWU31_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1083115.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRVVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64560..64898
                     /locus_tag="PWU31_000546"
     CDS             64560..64898
                     /locus_tag="PWU31_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL1083116.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            65017..65577
                     /gene="rsmD"
                     /locus_tag="PWU31_000547"
     CDS             65017..65577
                     /gene="rsmD"
                     /locus_tag="PWU31_000547"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL1083117.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65574..66065
                     /gene="coaD"
                     /locus_tag="PWU31_000548"
     CDS             65574..66065
                     /gene="coaD"
                     /locus_tag="PWU31_000548"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL1083118.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            66055..67098
                     /locus_tag="PWU31_000549"
     CDS             66055..67098
                     /locus_tag="PWU31_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL1083119.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67257..67985
                     /locus_tag="PWU31_000550"
     CDS             67257..67985
                     /locus_tag="PWU31_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL1083120.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            68101..68346
                     /locus_tag="PWU31_000551"
     CDS             68101..68346
                     /locus_tag="PWU31_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1083121.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68343..68561
                     /locus_tag="PWU31_000552"
     CDS             68343..68561
                     /locus_tag="PWU31_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL1083122.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68697..69236
                     /locus_tag="PWU31_000553"
     CDS             68697..69236
                     /locus_tag="PWU31_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL1083123.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69229..71502
                     /locus_tag="PWU31_000554"
     CDS             69229..71502
                     /locus_tag="PWU31_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL1083124.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71540..72586
                     /gene="holA"
                     /locus_tag="PWU31_000555"
     CDS             71540..72586
                     /gene="holA"
                     /locus_tag="PWU31_000555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL1083125.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVDEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72675..72929)
                     /locus_tag="PWU31_000556"
     CDS             complement(72675..72929)
                     /locus_tag="PWU31_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL1083126.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73329..73598)
                     /locus_tag="PWU31_000557"
     CDS             complement(73329..73598)
                     /locus_tag="PWU31_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083127.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(74068..74523)
                     /locus_tag="PWU31_000558"
     CDS             complement(74068..74523)
                     /locus_tag="PWU31_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL1083128.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74661..76589)
                     /locus_tag="PWU31_000559"
     CDS             complement(74661..76589)
                     /locus_tag="PWU31_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL1083129.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(76786..77619)
                     /locus_tag="PWU31_000560"
     CDS             complement(76786..77619)
                     /locus_tag="PWU31_000560"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL1083130.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(77811..78515)
                     /locus_tag="PWU31_000561"
     CDS             complement(77811..78515)
                     /locus_tag="PWU31_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1083131.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(78538..79164)
                     /locus_tag="PWU31_000562"
     CDS             complement(78538..79164)
                     /locus_tag="PWU31_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1083132.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            79490..81865
                     /locus_tag="PWU31_000563"
     CDS             79490..81865
                     /locus_tag="PWU31_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL1083133.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(81889..82380)
                     /locus_tag="PWU31_000564"
     CDS             complement(81889..82380)
                     /locus_tag="PWU31_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL1083134.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            82567..83487
                     /locus_tag="PWU31_000565"
     CDS             82567..83487
                     /locus_tag="PWU31_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1083135.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            83641..83985
                     /locus_tag="PWU31_000566"
     CDS             83641..83985
                     /locus_tag="PWU31_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL1083136.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        27279 a        14564 c        18080 g        24191 t
ORIGIN      
        1 ataatttaag ttaaccttaa aactaaactt cttaagtttg gttttaaggt tttttttgtg
       61 ttctagtaaa aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat
      121 aagtaacaat gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt
      181 ttttattgtg aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt
      241 cgatcgactt tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata
      301 ttattagggt ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta
      361 tcgaaagtcg tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat
      421 atcaatacag aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat
      481 attattctta ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca
      541 acgttgcgtg tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt
      601 gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc
      661 tttttgtatt cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc
      721 aaagcgatgt tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg
      781 ttccaagggt atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta
      841 gcgtttttgc gaattgacca gactgcaccg tggctatttc agccgatgga ttatttagct
      901 ggtttaagtt caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc
      961 aaaaaagctg catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta
     1021 cctgcattga atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat
     1081 tttgaagcac tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca
     1141 gcctatgcta tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca
     1201 actatttttg ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc
     1261 ataggtttat tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt
     1321 cgtccgaacg agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt
     1381 tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg
     1441 ttgaggggaa attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt
     1501 cgttatgttt ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat
     1561 tttggtatga cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct
     1621 ttgacttttg ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc
     1681 aaagattttc gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg
     1741 atcataggaa caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc
     1801 aaagtgatcg gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac
     1861 tcgcttgaag agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca
     1921 ggtgaaaacg atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc
     1981 ttgattaata cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa
     2041 agcaatcatt tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc
     2101 cgatccttca agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg
     2161 taccctagag ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat
     2221 atgatttcga caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat
     2281 gaggtatctc ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa
     2341 aatgtttaaa aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta
     2401 tgggataatt cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat
     2461 tcataacagg ttagcggccg gccgatttcc aaatatttga ttctttcgtt caatatatct
     2521 tgttctacta aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga
     2581 gccagttctt ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt
     2641 aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga
     2701 gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt
     2761 gtctgagaga aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt
     2821 ttgatcaaat aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct
     2881 gcagcagtga tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa
     2941 tggataccac tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt
     3001 ctgaaatatt tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg
     3061 atgctttcca caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg
     3121 acatttgtca tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc
     3181 atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag
     3241 attcaatcca aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc
     3301 tgagatttct tctgatatcc cgacaccgtc atcgcagacg gtcacgatga atacttcagc
     3361 ttctgtgtca tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac
     3421 ggcttctttt gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc
     3481 atccatctcc attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc
     3541 tttggcttcg tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact
     3601 tgagacttgg ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac
     3661 ttcttcatac ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc
     3721 atgggtttga gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa
     3781 tttcattact tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct
     3841 aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg
     3901 aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat
     3961 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
     4021 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
     4081 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
     4141 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
     4201 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
     4261 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
     4321 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
     4381 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
     4441 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
     4501 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
     4561 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
     4621 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
     4681 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
     4741 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
     4801 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
     4861 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
     4921 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
     4981 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
     5041 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
     5101 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
     5161 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
     5221 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
     5281 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
     5341 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
     5401 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
     5461 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
     5521 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
     5581 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
     5641 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
     5701 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtatacggaa caaggcgtac
     5761 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
     5821 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
     5881 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
     5941 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
     6001 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
     6061 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
     6121 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
     6181 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
     6241 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
     6301 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa
     6361 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
     6421 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
     6481 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
     6541 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
     6601 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
     6661 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
     6721 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
     6781 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
     6841 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
     6901 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
     6961 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
     7021 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
     7081 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
     7141 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
     7201 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
     7261 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
     7321 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
     7381 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
     7441 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
     7501 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
     7561 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
     7621 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
     7681 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
     7741 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
     7801 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
     7861 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
     7921 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
     7981 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
     8041 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
     8101 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
     8161 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
     8221 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
     8281 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
     8341 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
     8401 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
     8461 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
     8521 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
     8581 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
     8641 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
     8701 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
     8761 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
     8821 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
     8881 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
     8941 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     9001 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
     9061 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
     9121 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
     9181 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
     9241 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
     9301 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
     9361 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
     9421 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
     9481 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
     9541 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
     9601 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
     9661 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
     9721 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
     9781 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
     9841 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
     9901 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
     9961 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
    10021 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
    10081 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
    10141 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
    10201 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
    10261 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
    10321 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
    10381 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
    10441 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
    10501 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
    10561 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
    10621 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
    10681 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
    10741 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
    10801 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
    10861 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
    10921 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
    10981 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
    11041 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
    11101 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
    11161 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
    11221 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
    11281 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
    11341 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
    11401 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
    11461 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
    11521 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
    11581 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
    11641 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
    11701 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
    11761 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
    11821 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
    11881 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
    11941 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
    12001 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
    12061 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
    12121 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
    12181 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
    12241 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
    12301 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
    12361 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
    12421 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
    12481 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
    12541 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
    12601 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
    12661 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
    12721 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
    12781 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
    12841 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
    12901 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
    12961 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
    13021 caaaaaaatg tcttgattta ttaaagcgct ttctatagaa tgaaaaacga ataaaaacta
    13081 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
    13141 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
    13201 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
    13261 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
    13321 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
    13381 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
    13441 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
    13501 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
    13561 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
    13621 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
    13681 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
    13741 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
    13801 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
    13861 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
    13921 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    13981 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    14041 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    14101 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    14161 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    14221 tttccagctg cgtaaaaatc tggtaaacaa aggggcccat ccagaaccag gtggtggagc
    14281 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    14341 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    14401 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    14461 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    14521 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    14581 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    14641 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    14701 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    14761 agtaacagca gaagaagcag cagagagtct ttatgagttt gcgtcaaaca ttctaggata
    14821 gcagcaggag gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt
    14881 ggtcggttac gtatttgctt ctccattcat cctaggattt ctattgataa cagtgatacc
    14941 gatgatcttg tcactttatt attcgttcac cgattatcgt ttgggaaatc cagtctcatg
    15001 gatcggcttg gataattata taaagatgct gagtgatgcc cgttttttga actccatccg
    15061 agtcactttt ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg gtctattgat
    15121 tgcttatctt ttaacgagga atatcaaagg aacgactttc tttcgttctt tgtattatgt
    15181 gcctagtttg atcggtggca gtatttctat cgctctagtt tggcgggagt tgttttctaa
    15241 aaacgggtta gtgaatattt tcctggaacg attaggcgca cctgcgcttt cctggtttag
    15301 cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct gcttggcaat tcggttcggc
    15361 aatgatcatc tttgctgcag gtatcaaaga gatcccaggc tcgtattatg aagcggcaga
    15421 gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc acgttacctc ttctttcgcc
    15481 gatcattttg tataacttgg ttatgcagac aatcgcggcg tttatgactt ttacgcaggc
    15541 atttatcatt acccaaggtg gtccgaatga tgccacgaac ttttatgctc tttatgtgta
    15601 taaccaagct ttttctttta attctatggg gtatgctagt gctatttcgt ggatcatgct
    15661 gttgattatg ggagctgtca cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt
    15721 gtgaatgaga ggaggaagaa atgaataata cagtaacgaa atggtttcaa tacggtttta
    15781 ttttgctatt aggtctagtc atgatttatc cggtcttatg gatgatcgca ggttctttta
    15841 aaacggatca agagattatg agtggttcgc tgaatttgat tccagagact tttcaatggg
    15901 aaaattacgc aaaaggatgg gcggggtttg ctggaatttc attttttact ttctttagga
    15961 attcgtttgt catctctgca gtttcgacga tagggacagt tctgagctcg acttgtattg
    16021 cttacgcatt atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag
    16081 taacgatgat gattcctgca caagtgattt tgattccgca gtttattatt tacaatcggc
    16141 tgaatttggt tgggacgtat gtgccgctga ttttaccaca tttttttgga caagcatttt
    16201 tcatttacca gatcatgcaa ttcatggtca gcatccccaa agagttagat gaatcagcga
    16261 ttattgacgg atgcagcaag tatagtgtct ttaccaaaat tatttttcct ttgctgaaac
    16321 cttcgatcat cacgacgatc atcatccagt tttattggaa atgggacgac ttcatgggac
    16381 cattgattta tttaaacaag ccgagatctt acacggtatc gattgccatc aaattatttg
    16441 cagacgctgg atctagtacg acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc
    16501 taccggtttt tctgattttc ttattcttca ataaatatct tgtccagggg atcagtacaa
    16561 gcggtctgaa gggctgaata tgaaaaagcc aatagaagag atgtttcttt acaagctcaa
    16621 ctactatgtc tgtcggattt ttttaaccag tcttatcagt aatgctcttt tcgctttagt
    16681 tctaggatgt ttgttcactt taggattagt gctgtctgct cgtgttgtct atctgattta
    16741 ttttttgatg atgtatcctt cttattatct atctgtccat tacttggcag aaaaaaaggt
    16801 atgttggagg aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt ttcagctgtt
    16861 tgtcattatt tttgcagttg attggtattt attagtggga aagattggag cttggtatct
    16921 gattcctttt ctattgtgtc tgttgctttt tgtatcccat agttttttta gtacgctaat
    16981 tgatatctgc aaagaagata aagcagatag tcttgtttat ttgatcaaaa ggaatatctg
    17041 gctgttgtca aaaaacgtca aggggtttct gtggtattta tgccttttgt atggactgtt
    17101 ctggtgtata cagcatgatt ttttactcac tgcattactg tatccagggc tgccatttgt
    17161 cacagtattt tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa
    17221 gtcggacttg gtgggagagc gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa
    17281 acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg
    17341 atacttgtga acaagtatgg acagaaaaaa gtaaagacat gtatgccatc acaatttgag
    17401 caactactgg atgacgtacg gccggctgaa gtcattatta cgtcagtaga tcgaacgcat
    17461 catgattata tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg
    17521 actatcgatg aggaaaaagc tcaagaaata ataacggtcc aaaaacagac gggaagaagc
    17581 ctgcgagtca cttttaatta tcgttatgca ccgtatcaca cgaagataaa ggaactgata
    17641 gaaagtgatg tgattggtga tgtgttttct atccacttcg aatggctgtt aaatacgcaa
    17701 cacggggcag attatttcag aagatggcat cgaaataagc acaatagcgg cgggcttctt
    17761 gttcataaat ctacccatca ttttgatcta gtgaattttt ggttgaacag ccgtccccaa
    17821 aaggtatttg cattcggtgg attaaacttt tatgggatgc ataatgcgga gaaacgcgga
    17881 gtagaaatat tttacagtcg atgctatgga agcgaactag ctaaagctga tccatttgct
    17941 atagacctgt cagccaatga ggaattgcga gaaatgtatt tagaagcaga aaaagacagt
    18001 ggatatattc gtgaccgcag tgtattttcc gatgatatct ctattgaaga tacactaggc
    18061 gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg
    18121 tgggaaggat tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc
    18181 gaaaagtctt acatcaatgc aggtggagat aaaaaggatg aaggagcagc cgttcttcag
    18241 tccatccatg tctatccgat gttcggtgag ccatatgagg taatcgtcga acaagaagcg
    18301 ggcggacatg gcggaggaga tccgcgttta ttagatgatc tatttggtga agaaaaagaa
    18361 gaagatccgt ggaatcgggc agcaactcac gtagatggta ttttgtcgat tttgacagga
    18421 atcgcagcaa atcattctat tgcttcagga aaagcagtag tgattgacga gcttgtcagc
    18481 ttaaaagaat aagaatagat ttacggaaat tacttcccgt agtttataaa atcttgcctt
    18541 attgccgata ggctgtcttt tgaacactat ttattcggaa aaaggggcta tgacatgctt
    18601 tttgtcatgc cttcttttta tgtgggctgc ttttttagat aggagaaaat aaaaataact
    18661 atttaaaccc gaacaaaaag gaataatgca ttaaaaatca ttagatttta caaaaaaata
    18721 agtaaaccac gttgtcagtt tcagaaagta gtgttagact gttttcaata tttgaaacaa
    18781 cattttttat tcaaatatgg caaactgcct tcacgtgcaa aacagttgca agaaaatgaa
    18841 tttacactat tattagtttt acatacctac ttatatatcg tcataatatg gatgacagtt
    18901 tctagccagt accgtaaatg ctggactata agtaaaagat tggctatttc acccttcttt
    18961 tcttatgata aagtggaggg tgtttttctt taacagaaac atagtaggtt tttgcacaaa
    19021 agcggttatt tcggtattaa taaatgataa aggagcaaat aaagtgaaag aattagttga
    19081 acgtattcaa aaagacggac gtatattagg cgagggcgta ttaaaagtcg acagcttcgt
    19141 cactcaccaa gtagaccctg tattgatgga acaaatggga aaacggtttg cagaagtatt
    19201 taaagaacaa ggaatcacga aagttgtgac cattgaagct tctgggatcg caccagcttt
    19261 atttgctgca agagaattag atgttccaat gatcttcgct agaaaagcaa aaagtttgac
    19321 gatggatgaa gaattattga cagcttctgt ttattctttt acgaaacaag tgacaagtac
    19381 gatttctatt tctcgtaaat ttttatcttc agaagacaaa gtattgatta tcgacgattt
    19441 cttggcaaac ggacaagcgg caaaaggatt gatcgaatta tgccagcaag ctggagcgca
    19501 agtagaagga atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc agttacttga
    19561 agaaatggga ttgagagtcg tttctcttgc gcgtattgct tccttgacga atggacaagt
    19621 tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg
    19681 gttttagggc tgcagcatct gctagcgatg tatgcaggag cagtagcggt gcctcttttg
    19741 atcggtacag gactaggctt taatcaagaa cagatgactt atttgatttc aattgatatc
    19801 tttatgtgtg gattagcgac attattgcaa ttgacagtta accgattttt cggaattggc
    19861 ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc cactgatctt gatcggaacc
    19921 aatgaaggag taggagctat ctacggatct attatcgctt ctgggatctt tgttgtgtta
    19981 gtttctggtg tcttttctaa aattaaaaaa ctattccctc ctatagtaac aggaacggtt
    20041 atcaccgtta ttggtttgac tttgattcct gtagcaatcg agaaaatggg tggaggtaat
    20101 gcaacagcag ctgattttgg tgacaaaaca aatctgctac ttgcatttgt gacgattctc
    20161 ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc ggtattgatt
    20221 ggtttagtcg gaggaagcat cttagctgct ttcatgggaa tgattgatct gagcgcgatt
    20281 ggagatgcgc cgttgttcca tgttccacaa cccttttatt ttggtaaacc aactttcgac
    20341 gtatggtcga ttttactaat gatcctcatt tccatggtca gcatggtaga atcaacaggt
    20401 gtatattttg cacttggtga cattactggg aaaaatgttg gagaagaaga attgaaaaag
    20461 ggatatcgtg cagaaggact tgccgttatc ttaggcggta ttttcaatac attcccttat
    20521 actggctttt ctcaaaatgt cggattggtt caactttcgg gaatcaaaac aagaaaaccc
    20581 atctatttct ctgcattctt cttgattgtt ttaggattat tccctaaaat cggtgcgtta
    20641 gcacaaatca tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg
    20701 gcagtccaag gaatccgtat gctgttggaa gttgatttca gcaatgataa aaatctattg
    20761 attgttgctg tttctgttgg atttgggctg ggtttcaatg ttacgccgac attattcagt
    20821 caaatgccgc aaacattgca aatgttcact ggaaatggca tcgtaatgag tagcattacg
    20881 gctatcatat tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat attggaagaa
    20941 gccttcaaga agtaatagag caaaagaaag gttgtgaaat cagcgttttg acctgattgt
    21001 tagagaaaca aacggaaaaa tagttcctcg tattttgccg tttgtttgag ctaataccga
    21061 gggtcagctg attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt catctccgag
    21121 tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac
    21181 cgaggagttg gcaggagaac accgtttatc cataaaaagg aatgtgacct aacttttgtc
    21241 acattccttt tttttatctt taataaacac gaacaataaa atgtttttct ttgttaacat
    21301 tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg
    21361 aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag attgggaaac
    21421 aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt
    21481 ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg
    21541 aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc
    21601 aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atggtttgga
    21661 ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg
    21721 aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt
    21781 agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag
    21841 tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg
    21901 gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc
    21961 tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct
    22021 atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat
    22081 tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag
    22141 ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga
    22201 ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga
    22261 ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg
    22321 ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag
    22381 gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag
    22441 gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata
    22501 ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc
    22561 gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt
    22621 ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg
    22681 gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt
    22741 gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt
    22801 tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc
    22861 atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga
    22921 cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga
    22981 gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga
    23041 acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg
    23101 gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc
    23161 ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt
    23221 ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg
    23281 tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga
    23341 aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg
    23401 gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt
    23461 attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag
    23521 gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg
    23581 tatgtaaaca attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc
    23641 tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa
    23701 cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag
    23761 gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg
    23821 ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat
    23881 ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct gataccacga
    23941 ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc
    24001 ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta
    24061 tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa
    24121 aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa
    24181 ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt
    24241 tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca
    24301 aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc gttagcggtt
    24361 ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt ttaaaagcgg
    24421 ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg taaatggctt
    24481 ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg agaaaagcgt
    24541 attccatctc ttgtccattg aataagatct ggatacgata atgatttttg aaacgcttct
    24601 gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa agaaaagtcc
    24661 ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat gtataaaaac
    24721 tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta tatagacagg
    24781 aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt gcatcttcca
    24841 tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa tataccatca
    24901 ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga taaaacacga
    24961 acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata aaaagatgat
    25021 tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt aatgtaaata
    25081 aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag ccaaaaaaat
    25141 atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag caactgcgtt
    25201 gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc aaatcacaag
    25261 tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg aacaattgtc
    25321 taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg ttatccgaaa
    25381 tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag aattcatcaa
    25441 accaatcatt gaattctact ataaggatga ccagttggat gatccattta tcaatgaaga
    25501 gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga aagcccaagc
    25561 gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg ttttagtaga
    25621 tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg aagtaacacc
    25681 ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag acgtttaccg
    25741 cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc tatcggaact
    25801 aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac ctataaagat
    25861 tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat gggatatgaa
    25921 acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga tgcagaagaa
    25981 ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt aaatatggaa
    26041 acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg cggtcatcgt
    26101 ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga tcatgggcgc
    26161 tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg gggttttact
    26221 gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc ggttttctac
    26281 aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg gtacatgcaa
    26341 tggattccag atattgacag aagcaggact tttgccaggt gtactgagac gaaatgagtc
    26401 tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg cctttacttc
    26461 agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag gaaattattt
    26521 ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct ttacttataa
    26581 tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag cagggaatgt
    26641 gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat cagaagatgg
    26701 gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat agatgaaaac
    26761 attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg aatggggatt
    26821 gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc ctaattatac
    26881 ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa attctaaacc
    26941 ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg gagaaggcgc
    27001 aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa gtcacaatca
    27061 tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa tcatcagaga
    27121 tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt tcggcgaatt
    27181 agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg gcggatacgg
    27241 caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt gttatgaagg
    27301 aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata tccagaaagg
    27361 gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag gtcgcgacgg
    27421 tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac agcaacgttc
    27481 tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt gtttggaatt
    27541 aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag caggacttgt
    27601 ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga cattagatga
    27661 agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg aatcacaaga
    27721 acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac tatttcagaa
    27781 gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat atcggctata
    27841 tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag atgcaccagt
    27901 ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt tagctcctta
    27961 tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc aaccgacgat
    28021 tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca atacagttgt
    28081 ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag cgttagctat
    28141 gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag gacaaatagc
    28201 agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg cgatcaccga
    28261 ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat cgacttcagc
    28321 tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag gaaatgtctc
    28381 tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg gtatggtcgg
    28441 attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag gggatctaat
    28501 ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa agatgcagct
    28561 tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga gaaaccaaga
    28621 gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact gttcagaagg
    28681 tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga tatctgtaca
    28741 gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg tgttgacagt
    28801 cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg ttttagcagg
    28861 taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga tcaaaataga
    28921 aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga agtgaagagt
    28981 ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc tagtgtggca
    29041 tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat cgtgtccaat
    29101 gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt attcaaagat
    29161 caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg ttatgcaaca
    29221 gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca tgatcagtcg
    29281 atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa agagttagag
    29341 gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca tttgatccga
    29401 cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga agtcaaaggt
    29461 ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga cccaaatggt
    29521 tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc ctcggaaact
    29581 tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg tgagatagtg
    29641 atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca gctttctatt
    29701 tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg gatcaatgtg
    29761 catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt aaaagcagat
    29821 atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc ggaagctagt
    29881 ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac atttatccag
    29941 ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt acgaggagta
    30001 gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac gacaagcaga
    30061 cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag gattgcttct
    30121 cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa agaactcatt
    30181 gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc tttaggtttt
    30241 ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc gtattcagga
    30301 ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta tgaagaaaaa
    30361 tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa gaagggagta
    30421 tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag ctggatatga
    30481 agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta tgatgggcgc
    30541 tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag agccagtcct
    30601 tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag cagacaaaca
    30661 cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag cacaaggcgc
    30721 agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt tacgtttgga
    30781 aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc tgattggcgg
    30841 agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag ccggatttgc
    30901 tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc caggagatgt
    30961 gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg ttcgcaaaat
    31021 tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga aaaaaacact
    31081 tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc cgctgatcaa
    31141 agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag aaaatatccc
    31201 gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc cgattcttcc
    31261 aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt atgaaatctt
    31321 caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag aaatagtaca
    31381 cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg ccaaagaaaa
    31441 acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc ttctggaaac
    31501 ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga atcttcgatt
    31561 gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac tgcgttgagt
    31621 gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta tgaagaagca
    31681 attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg atatatgcga
    31741 atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat ccatccgtct
    31801 cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc tggagtaaaa
    31861 gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc aatcatccgg
    31921 caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga aaaaatccat
    31981 agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa tggaggctat
    32041 ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta gcattcgccc
    32101 aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag catgcgttgg
    32161 ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct gaaatgatgg
    32221 atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga agacgcgatc
    32281 tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac tttgtttgtg
    32341 tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata gcagaagcta
    32401 ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa aaccatgaat
    32461 ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa ttacgtcagt
    32521 atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt cgtcatactg
    32581 cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa aaagaaccgg
    32641 aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa aatagccatc
    32701 aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca gcagcaaaac
    32761 aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct gcgctacgga
    32821 tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat ccttgtggta
    32881 ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca gatcctgttt
    32941 caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca gcagaacaga
    33001 tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa gctttggaat
    33061 tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag gaagaaaatc
    33121 aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat caagatctgg
    33181 caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact ccagcagaag
    33241 caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat gcgatcgtac
    33301 tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga gtcggttcag
    33361 taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta gctagcgatg
    33421 cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata aaagcaatca
    33481 ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat aaatacgatc
    33541 tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc ggataaatga
    33601 acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa cttttagcca
    33661 gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca aaagatggta
    33721 tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt gttaagaaag
    33781 aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg atcgtcgatg
    33841 attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct ctgatcgaag
    33901 gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca gctaaatcca
    33961 gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt gcgccaatcg
    34021 tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc acagtgaagg
    34081 aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat agtagcaaaa
    34141 aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg tttgtccgta
    34201 ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt gataacgata
    34261 aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt tcaaaaaaga
    34321 tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg aaagaactag
    34381 gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa ggaccaaaag
    34441 tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt tctcggttga
    34501 agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta gagttaaagt
    34561 ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac cccgaagaat
    34621 atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt tactatgctg
    34681 gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat ttagtagaag
    34741 catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta atggataaaa
    34801 cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat agcgaactga
    34861 tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa tcggaagcgc
    34921 cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt atataaataa
    34981 ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc agctgcagtg
    35041 atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat tgagacagct
    35101 tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg tgaatgtgaa
    35161 gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct ttttattcaa
    35221 acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca tatcattgaa
    35281 tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt acttcttcat
    35341 cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga attggaacgg
    35401 tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat cgaattgctg
    35461 aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt gaaacatact
    35521 ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat cgatcatgac
    35581 ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg gtcaccatac
    35641 cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga attaaaccaa
    35701 acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc ttctgcttgg
    35761 attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt agggcgaatc
    35821 aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta cgaattgtac
    35881 cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa gggctataaa
    35941 ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg tcttttttta
    36001 ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt tgtttttaag
    36061 gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa ataaataact
    36121 gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac gatagtctga
    36181 ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat cgatgaatgt
    36241 ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg caacttgttg
    36301 agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa aatccaatgc
    36361 atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag cagttaagaa
    36421 agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga ttgctaagcg
    36481 ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt tcaggcagac
    36541 atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag ttggtgtgcc
    36601 ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata atgaagacaa
    36661 atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt cttgaatcaa
    36721 atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta tcatctgcgt
    36781 gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa tggcgacaga
    36841 aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta cctgataact
    36901 ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga atcgagagga
    36961 aacagtagag aagatcactt gttgattctc cctaaataaa aacttcaaat aatcactgag
    37021 atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata cggtcatttt
    37081 tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac tgatcgtaag
    37141 tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa caagatcaga
    37201 agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt ctattgcttg
    37261 tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta cctggattgc
    37321 tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag tccaaggaag
    37381 tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt ggatatcagt
    37441 cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac catctccagc
    37501 ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt cccgcaagtt
    37561 attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg tttcctccaa
    37621 ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg tatttgctgt
    37681 caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg tttacgttgt
    37741 aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct ttacgtattt
    37801 ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat tggatcatct
    37861 catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac ttcgttccat
    37921 ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa acaggaaaaa
    37981 gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt tttaagatga
    38041 ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct tgtcctttgc
    38101 ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct tcttcaaagc
    38161 agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa tggggagtaa
    38221 tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc aaagcgttct
    38281 cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta agtgtttgta
    38341 attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa agttgttctg
    38401 acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag cgacgattct
    38461 ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt aatagaatga
    38521 gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca cgaacaaaat
    38581 cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg atctcttcat
    38641 tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga taatcttcca
    38701 gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt gtatttgtat
    38761 tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc tcattgaact
    38821 catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact ttttgatcaa
    38881 ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga taaatcaaaa
    38941 gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat agaagcatgg
    39001 ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg atacttctcc
    39061 agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac agtctcggaa
    39121 ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata acgagtaacg
    39181 gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt gacgattcct
    39241 gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat caaatcttct
    39301 ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat ttcatcacta
    39361 tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg tttcatctgg
    39421 tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt catcggaggt
    39481 ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga ttccaaaaat
    39541 ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc taggattctg
    39601 atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct
    39661 tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt cgtttgaata
    39721 tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa tacagagtcc
    39781 cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc aatgaacgtc
    39841 cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag aacttggtaa
    39901 tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca gctactctcc
    39961 gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt attaggtgta
    40021 caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac acgtttcact
    40081 ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg aaaatcaaag
    40141 tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt
    40201 acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct ttctttatta
    40261 gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga
    40321 ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag
    40381 gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa
    40441 gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt tataaaacag
    40501 atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa aatatgatta
    40561 caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta actgatgtgt
    40621 tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta ctgatgaatt
    40681 cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta tcgcaagcag
    40741 cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg attgaattat
    40801 ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg atgtctgttt
    40861 tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg agtttcagtc
    40921 aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat aatctgttaa
    40981 gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat ccaataagtt
    41041 taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg
    41101 tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc ggggaacaaa
    41161 cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa atgatcaaag
    41221 caatcagtga tggagaagag gttccggtca atgatactga aacttatgat aatggggtga
    41281 aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat tatcaagcag
    41341 agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag gaaccaaaca
    41401 aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt gtattttaaa
    41461 aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg tgaatctgat
    41521 gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa aatcaacatt
    41581 gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa tcttttatga
    41641 tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat
    41701 tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt ttctcggaaa
    41761 tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa catatgaact
    41821 gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg
    41881 gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc tgttgatttt
    41941 ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga gattgatccg
    42001 agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga atgaaatcgt
    42061 ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga cactagaaaa
    42121 tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt tagttgatcg
    42181 ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag actggacggt
    42241 ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc atttgaaaaa
    42301 aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat tcgctatgag
    42361 tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg aaggggcaga
    42421 gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg tctcggagga
    42481 ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga cgctgagcag
    42541 tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa ctgaggcaga
    42601 aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg tcagtagtct
    42661 tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac aacccgatat
    42721 tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg agatctacac
    42781 gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt ctgaacttcc
    42841 tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta ttacaggaga
    42901 agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga aagaagaggt
    42961 gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa ctaaatttga
    43021 aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta gcgggactac
    43081 ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat ataactaacc
    43141 ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta gttgtgctct
    43201 taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca attgcgggca
    43261 tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt cttgcagtag
    43321 gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata ccggcgttta
    43381 tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt ctaggaggcc
    43441 aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat ttaccagact
    43501 taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc atcacgatcg
    43561 tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg
    43621 aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta ctaggaataa
    43681 cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt ggtttgatcg
    43741 ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt tatgcgacgg
    43801 gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc acgttctggg
    43861 tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct gctcgattga
    43921 atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg gcagtatatt
    43981 ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt gtcggtggct
    44041 tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta gactggcagc
    44101 aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat aataagaaaa
    44161 aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa gacatttgtc
    44221 acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga ctaaaaataa
    44281 tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt ttgcttaatt
    44341 gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt
    44401 gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga agatattcaa
    44461 ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac ggtcacatta
    44521 ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat tattgatgaa
    44581 acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg gtttgatgaa
    44641 gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt
    44701 gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga atcgctaaat
    44761 cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat tgatatgatt
    44821 cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt tggtacaccg
    44881 gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca
    44941 ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg gcaactggca
    45001 gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg tgggatcaga
    45061 acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat gattggttca
    45121 ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat
    45181 aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga
    45241 aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga aatgcaacaa
    45301 gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat tcgaaaagtt
    45361 gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa aataaataaa
    45421 atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc caaacaaaaa
    45481 tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat
    45541 cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa
    45601 aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa
    45661 aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat cgtcaaatta
    45721 tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt caacttgaat
    45781 cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc catgcattgg
    45841 aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg tttgttaatc
    45901 agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg atctctcctc
    45961 gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag atccaaaaaa
    46021 catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg aattttgagt
    46081 ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat cttttaggct
    46141 atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa ttggcatttt
    46201 cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg attttacaaa
    46261 cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt taccgattac
    46321 ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa gaagggatcg
    46381 acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg cttatcaacc
    46441 cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag
    46501 aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg gatctttcat
    46561 ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat gtgttatacg
    46621 tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg ataggacctg
    46681 agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg atgaatatcc
    46741 ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa atgcaagatg
    46801 taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat tcacaatcgt
    46861 ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg gatgggaaaa
    46921 aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt gcaccaagtc
    46981 tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg gatgaaaacc
    47041 aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca gaataatttc
    47101 acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc tgtttctttt
    47161 ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt gtacgtccgt
    47221 tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca acgtttttaa
    47281 atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat gaaatataaa
    47341 acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt ggcataattt
    47401 cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca tgataattta
    47461 ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg cagaatgaag
    47521 agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa agcaccgatc
    47581 gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca agcacttgat
    47641 caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga tgaactcgtt
    47701 gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac agcgctgaac
    47761 cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag tcagctggct
    47821 agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga tgtacctcaa
    47881 ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg acatgctgca
    47941 ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat cggtgcacaa
    48001 tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa aaatgtcgtc
    48061 ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc aatcaatttc
    48121 gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta tgcgatctct
    48181 acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt cgcagctggg
    48241 attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt atctaaaatg
    48301 gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact gacttatcgt
    48361 tatggaccac atacactatc tggtgacgat ccaactcgct accgtacaaa agacacagat
    48421 gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac ggaaaaagga
    48481 ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga aatcaaagca
    48541 gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt gaaaaacatg
    48601 ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc gaaggagtcg
    48661 aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta gcacttgaac
    48721 tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac ggaggagttt
    48781 tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc gatactcctt
    48841 tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga tttcgtccag
    48901 ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta gtagctcaaa
    48961 tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca attcgttcgc
    49021 catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa ggtttgatcg
    49081 ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca aaaggactat
    49141 tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg aaactatatc
    49201 gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat aaagcagcag
    49261 ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg cgtgaagcaa
    49321 tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt gatcttcgta
    49381 cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact ggtcgcgtag
    49441 ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt tctgagatct
    49501 cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca cctgatacag
    49561 tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat atcgaagaca
    49621 aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc atggcttatc
    49681 aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg
    49741 tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat
    49801 cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt gttccagaag
    49861 gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga cacgaagaca
    49921 atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg gctgctgaac
    49981 ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa tttaagttac
    50041 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
    50101 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
    50161 ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc actgtagcaa
    50221 atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca ccgtcaacta
    50281 gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg
    50341 ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt caatttgccc
    50401 gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa
    50461 aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag
    50521 cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac
    50581 cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca atgacgccaa
    50641 cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca cacgtgacat
    50701 tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt aaagaagttg
    50761 ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct ttaacagcga
    50821 ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt
    50881 ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta tatgtaccaa
    50941 atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc aatgagaaag
    51001 caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga acaattacga
    51061 tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac tatccagaag
    51121 tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa
    51181 tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc gtagatggag
    51241 caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca gagctattaa
    51301 tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt agatacagtt
    51361 gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga aatgggacaa
    51421 aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt cggctgtatt
    51481 ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga ttctgaaatg
    51541 tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga atggaaagaa
    51601 aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa acataaggtt
    51661 gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt cattcatcca
    51721 gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc ccgtccaatc
    51781 gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg attagcattg
    51841 aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc
    51901 ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc acaaatttta
    51961 ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa aaaaggcgta
    52021 acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg
    52081 aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat ggtcactgta
    52141 ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga agtaggagaa
    52201 cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat ctatgccatc
    52261 ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc aaaaatcgct
    52321 gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc ggcagtagcc
    52381 ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa agatgctggt
    52441 atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt atcgcttggt
    52501 aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct tattggcgcg
    52561 caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc aatcgaatca
    52621 ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct tggcgaaatc
    52681 gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt tgaataatac
    52741 aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc aataatatta
    52801 ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg
    52861 aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag
    52921 attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga
    52981 atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg
    53041 tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt
    53101 ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg
    53161 ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga
    53221 gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct
    53281 agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga
    53341 tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg
    53401 aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa
    53461 tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga
    53521 cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga
    53581 gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta
    53641 tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg
    53701 tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta
    53761 tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg
    53821 tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt
    53881 agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa
    53941 aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa
    54001 aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa
    54061 aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac
    54121 gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat
    54181 gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg
    54241 gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta
    54301 gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc
    54361 gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa
    54421 ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca
    54481 atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa
    54541 gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt
    54601 tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca
    54661 accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc
    54721 gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc
    54781 ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa
    54841 ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa
    54901 cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat
    54961 atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac
    55021 attgacgaac caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga
    55081 gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca
    55141 gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt
    55201 ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa
    55261 gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa
    55321 cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta
    55381 cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc
    55441 ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt
    55501 tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc
    55561 ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc
    55621 atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga
    55681 atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa
    55741 caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa
    55801 caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca
    55861 ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct
    55921 aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag
    55981 ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc aatggacata
    56041 aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat ttctagagag
    56101 tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa
    56161 atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta aaacaatact
    56221 ttggctatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc attgatcagg
    56281 aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac cagcttccag
    56341 ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg atgaaggatc
    56401 aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc tcgttagatg
    56461 gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt ttactttata
    56521 tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga tggaacgtcc
    56581 gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat ttccgcccaa
    56641 gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta attgttgctt
    56701 tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta aaaattccag
    56761 aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa gtaattaaag
    56821 atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat caatctggta
    56881 tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt aaaaaattcg
    56941 atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca gaaatgcaag
    57001 aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt ggtatgggta
    57061 taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct ctagaagcat
    57121 actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc atcctgcttt
    57181 tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa agagaagaag
    57241 ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg tatattgagt
    57301 cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt
    57361 gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca cagatggttc
    57421 tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg aaagtcttag
    57481 caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct acctatggac
    57541 tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg atatcgaatg
    57601 gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa agagggatac
    57661 aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc cagcagctat
    57721 cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg gatctagcat
    57781 cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa gaaatgagcc
    57841 gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc caaagcaagc
    57901 ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct aacgttctgg
    57961 ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa tctgtttttt
    58021 ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga ggtaagcaca
    58081 gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag cagaacggat
    58141 caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca aagccttcga
    58201 agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat
    58261 aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac gagaattgaa
    58321 tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg gcaaggagga
    58381 atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat cttgattccc
    58441 tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc gtatctatta
    58501 ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt ttgggtgctt
    58561 agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa aaaccagcgt
    58621 ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt gttttttggt
    58681 aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat ccaacctgcc
    58741 gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa aagacaaaac
    58801 agtgtacaaa aaaattttct tgggactgtg aaacgtccac ttgcaatggt tatcggactt
    58861 actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt tttgattatt
    58921 ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt aggagtagga
    58981 ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg aaagatctta
    59041 cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc tttctcagac
    59101 gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa cggaggacta
    59161 tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga agcacaaacc
    59221 gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat tttgatatta
    59281 gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg
    59341 ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt atttgtcaat
    59401 cttggaggaa ttactggcgt tattcctttg acagggatca cgttcccatt tttaagtcaa
    59461 ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg
    59521 gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa
    59581 aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga
    59641 attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg tacaccgttt
    59701 caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga
    59761 tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc atccgggtta
    59821 cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag gcatcatttt
    59881 tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag caaaagctgc
    59941 agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg cttctataga
    60001 cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg cagctctagg
    60061 cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag agggctacga
    60121 acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata
    60181 catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg gaaacgtcat
    60241 ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag tagaagtagc
    60301 gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt
    60361 gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg aaggagatga
    60421 tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca cagaaatgat
    60481 tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg atctgcataa
    60541 agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca ttcaatgccg
    60601 aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa tcgatacgta
    60661 tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt
    60721 agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg ctacctttga
    60781 aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg gagtgaaaac
    60841 aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa
    60901 aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac gagaccgcgg
    60961 aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag gtatcgaaag
    61021 tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca caagaactga
    61081 ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg tcgaatggat
    61141 caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc atcaaagtct
    61201 gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga ctggagaagg
    61261 tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg tggcttaccg
    61321 ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac
    61381 gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt atcctgacaa
    61441 tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat ttcgaatctt
    61501 tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac gggatactgg
    61561 taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa gtcgggcaaa
    61621 atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg gtgcgcatat
    61681 cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc gtttgattag
    61741 tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata ccagcgggaa
    61801 tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg acgtggcaat
    61861 gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt atgcactggt
    61921 caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa accattattg
    61981 ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga
    62041 agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac aggcaaaagc
    62101 tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg tcaacttgat
    62161 gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata tggcactatt
    62221 tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa cactaagttt
    62281 ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg gcggatttcc
    62341 taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac gtccaggtct
    62401 tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta
    62461 tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct ttttggacta
    62521 tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa catttttcca
    62581 aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat
    62641 tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt tcttcaattt
    62701 gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta cggtccaaac
    62761 ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt ctggttctgt
    62821 tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac
    62881 tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag tcgcacatgt
    62941 atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag tgactgagaa
    63001 ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct gattctgcta
    63061 ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact tgaagtgaag
    63121 gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa
    63181 gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa ggattttggc
    63241 caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta tgaaaataaa
    63301 gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac tgttaaagta
    63361 ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat gcaagatctt
    63421 acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac agaagttgga
    63481 atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga taacgacact
    63541 tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt gtatttagat
    63601 aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct tccacattat
    63661 caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg
    63721 actttattaa atatgcttag aagagaagac gaacttccca tatacaatca aacacttgcc
    63781 tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga ggaaatattg
    63841 tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa
    63901 ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt agaaaatact
    63961 agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata ttggtacagc
    64021 gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg cattgctttt
    64081 tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag tgatagcagt
    64141 gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat
    64201 aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg aagaatctgc
    64261 aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc
    64321 ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagttgttc gaaaagcaaa
    64381 aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa cacagctgca
    64441 aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg aaatcaaagt
    64501 agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca
    64561 tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc gtatgggtct
    64621 acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat gtctcaagaa
    64681 gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca gaagaacgaa
    64741 tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat atcgaaatga
    64801 attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc gaaattgaag
    64861 agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg agcagttttt
    64921 aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta tttttttggt
    64981 atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc aggaaattat
    65041 ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac agataaagta
    65101 aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt attggatctg
    65161 tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag tcatgcggta
    65221 tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga aattacgaaa
    65281 gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga acggctagca
    65341 gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa acaaaaaata
    65401 atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc agttatcgta
    65461 tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca tcaaataaga
    65521 aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa tcaatgaaac
    65581 gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat acggtggaac
    65641 gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa
    65701 gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct catcttccta
    65761 atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa gagattggtg
    65821 cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa cgagagattg
    65881 ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta gcaaaaccag
    65941 aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc ggagatgtga
    66001 gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa
    66061 gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat cattggattt
    66121 gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa
    66181 ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct tacctctgtt
    66241 ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc ttttgaagaa
    66301 ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa tcaaatgcaa
    66361 cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac
    66421 aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa aacatcgaat
    66481 ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa aagctttcaa
    66541 agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc
    66601 agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga gttaccaaca
    66661 gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga atcagataca
    66721 gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat gttcacttta
    66781 gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact
    66841 ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa aaaagtggca
    66901 agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat ttcagacgat
    66961 gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc tgccgctaaa
    67021 aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc gcttgactat
    67081 ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga gttctgtcac
    67141 aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga aaaaattgac
    67201 gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa ttttaaatga
    67261 aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg atagaaaaaa
    67321 tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg atagttctta
    67381 tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga gaaacagaag
    67441 agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat ccatcagctg
    67501 aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat atcaaaggaa
    67561 gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat gatgtactaa
    67621 agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt tctgcgaaga
    67681 tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca gaacatcttg
    67741 atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct cccaaaataa
    67801 atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga tcagtcaaag
    67861 cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg gaagacctgc
    67921 aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc gttacaatta
    67981 aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg gatactaaga
    68041 ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat aggaaatagc
    68101 ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct ccaaaataaa
    68161 atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca agctctacga
    68221 gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat tctcattggg
    68281 acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa aatctgcagt
    68341 ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt atcacgccac
    68401 cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa gaatatatct
    68461 ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt atacggaatt
    68521 tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg gacttcattt
    68581 cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt aaagaatatt
    68641 ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata taaaacatgt
    68701 tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat aagtcacata
    68761 atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta tctttacgta
    68821 gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg atcattgctg
    68881 gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg tgttacgtcg
    68941 tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta cagtgtgcaa
    69001 aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca tgtttggcct
    69061 gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat gactatcgaa
    69121 atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac caagttgctt
    69181 tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat gtctagcgtt
    69241 ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat gattatctgt
    69301 ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat ttttttatgg
    69361 ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag cataacggta
    69421 tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac atctgcgcaa
    69481 attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat ggaaggaagg
    69541 ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga agcagaattg
    69601 aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg cgagttcatc
    69661 tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt caggaaccat
    69721 agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca aaatcactgg
    69781 cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc tgaatttccg
    69841 caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc aggattcaga
    69901 gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct atcgggtcta
    69961 cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg atggccacta
    70021 cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat tgcaggaagt
    70081 tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt caaattgctg
    70141 aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct tttactattt
    70201 gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag ttttttgttt
    70261 atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt cctgtttact
    70321 atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt aggtagtctt
    70381 tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt gttatttctc
    70441 acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga gggattaatc
    70501 ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg acgtttgccc
    70561 cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag gttgaaagaa
    70621 caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt gactttgcca
    70681 tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga cagtatcttt
    70741 ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca gttacacttt
    70801 gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa tatcgttcca
    70861 tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca cgacgatacg
    70921 gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa aatttatatc
    70981 ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc tcaaaaaaat
    71041 ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta tgatctttat
    71101 gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg tctatggatg
    71161 gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat ggaaaaacaa
    71221 ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca tcacggtagc
    71281 cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg gattatatcc
    71341 tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac actagaagaa
    71401 aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg ctggcatcca
    71461 gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc atctgctcat
    71521 ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt tacaaaaaat
    71581 acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt ttctccaaga
    71641 tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg acttaaattt
    71701 tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagatgaag cagagacgtt
    71761 gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt taacaggtga
    71821 aaaagtaaac aatggcatcg aacaaaatac tgatttattg acggcttatt tgaaagaacc
    71881 attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg aacggaaaaa
    71941 agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt tgaatgaaaa
    72001 agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa tggaacgaag
    72061 tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga tgagagagtt
    72121 acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag aagtagaaga
    72181 attagttcct aaaacgttag aacataatat ttttgacatg acccaatata ttttaaaagg
    72241 gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg aagaaacaat
    72301 caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa aatttcttgt
    72361 aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc catatcgtgt
    72421 aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac agttgtttga
    72481 tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag aattaagttt
    72541 tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag aaagacgagg
    72601 gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa aaaaatcggc
    72661 cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc gcggttagct
    72721 ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac agcttcttta
    72781 tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt tttgattgca
    72841 gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct tgtacgaaca
    72901 cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt aagttcttac
    72961 gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg ctttgcaata
    73021 tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt cagtaaaaac
    73081 ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat agcgtcaaac
    73141 caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt tggtactaag
    73201 ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat acgattccct
    73261 gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta tcctttgatc
    73321 agtaaaggct attctggtgt accgattatt ttaatgtatt catcgatatt ttttatcaac
    73381 atttttttta tgaccaactg gttccgacgg ttccatttgc cacttacttc cagcacatat
    73441 ttatttaggg gcaaacgtaa gatatatttt gccaaatagc gatctttaag caggccattg
    73501 atcacacggt cctcaagctg caagttgatc ccagtcagca tcattggctg ttcttgtaaa
    73561 acgtacgtat caattaaaag accttcaaaa gtttccacaa gcgtcttcct ttcttttcaa
    73621 agtaacctga aaacaggcac taattcttcc attttgatat tcgttgaagt ggatgcgatc
    73681 attctttctt ttgtccactg taatctagta taacaaaaaa gagcagaaga gccaaaaaga
    73741 aaaaagagca tcgaattcta taggaagtct cattggcacg gcctaaatta ttacaaaaca
    73801 ttgattaaaa aaagcccctg catcgcagga gcatccccta tatcgtttta ataggtcaat
    73861 caaaaagcag tatcaaacac attttgtatc aaatcaggaa ataatagata agctaagccc
    73921 agaatcaaaa gtaccgctag aagaaaccag ccgattgttc ctatgaaata ctttaatttt
    73981 tcgaacattg tatcacctcg ttgtcagtat atcacggatc agtaaattgg gggtgttaag
    74041 aatctcctaa aatagacaaa atttgttcta ttttcctaaa gcaaatactt gtaagataca
    74101 gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga agacaacacc
    74161 taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa tcaatcctac
    74221 acccttggaa gataagacag ccactagccc accgcaaaaa acagctaccc agccaatagg
    74281 ggatttaaag gcttctaata gatcacgtag accgatttct cctgttgcga tagggattag
    74341 aatagccgct gtgatgatcg tcaccccaat attgattcct ttgccttgaa gccaagggaa
    74401 aaaggaacgg gaggctggta tgagcttcat aatcaaaaca aaagctgccg ctatcatcag
    74461 actttgattt ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa gccaactttc
    74521 catgaaatat attcctcact ttcttcgaaa caatcataac atgtttttag aaagttacaa
    74581 aagtaggaat tcatccttca agacctattc ataagctgtt tttttcataa aataaacaaa
    74641 atcataaaaa aacactttgc ttatttgaca gaagacagcc gcgctttgta tagtggacaa
    74701 gacttacatt tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa acgcaagcca
    74761 ttcagaataa ccaatattgt gcttccttcg tgtcctacaa ctccaagagg tagatttaaa
    74821 aattgcagga agttagaagc aatcaacaag agaataacaa gaatcgaaaa aataatattt
    74881 tgtttgatga tccgccgcat ttttaaagat agccgatgac tgtagacaaa tttatttaag
    74941 tcgcttttca ttaagacgat gtctgcgata tccatagcgg catctgtccc ttgccccata
    75001 gcgacaccta aagaagcggc tgctaaagca ggcgcatcat tgactccatc accaaccatc
    75061 gcatttactt ggtaaagttc attctctttt ttgactagtt gtgttttttc ttccggagta
    75121 cagcttgcat agtaatgttc aatccctact tgttcagcaa tggcctttgc tgtgccagaa
    75181 tggtcgccgg ttatcatact tgtatggatg ttttgggatt taaagtagga aattacttgt
    75241 tgggtatttg cttttggaat atctagcaga cctaaaacag caattaactg ctgatcttta
    75301 gaaagataaa tcacggtttt gccttgattt tccaaacgtt cgatcatttc cgcaatctct
    75361 ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc caactttcca ttgcgcacct
    75421 aaataggtag tttgtaatcc aaaaccagta atttcttcta ttggtaagta ttggatttct
    75481 tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag caagtggatg cgtcgttttt
    75541 tgttccatag caacaagaag ttgttgtgcc aaatcctttt catcaaagaa aaagctgtca
    75601 gttacaactg gttttccttt agtgagcgtt cctgttttat caaaggcaat cgcttttaat
    75661 tcagaaagct tttctaaatg gataccgcct ttgattaaaa tcccattttt tgcagaattt
    75721 gaaagagcag ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg agaagccaca
    75781 actaataata ccattcctcg ataaaagctt tcatcccaac tccaacctag gaaaaagtgg
    75841 gggagaagga tcattagagg aacagcaagc aaaatcaatt ttacataatt attttcgatt
    75901 cgttcgataa atgaagctgt ttgagttggc gtattctgcg cttcttctac taattggata
    75961 atcttagcaa agacagtttc ggaactcgtt tttgttactc gaatcgtgat gggatttcct
    76021 tgattcagcg ttcctgcata catctcatcg cctaaatatt tttctacagg cacagattca
    76081 ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc atctatcgga
    76141 acagctgctc ctttagcgac aaataccaag ttatccacct ttagttgaga aacatctact
    76201 tcttgcattt gtccattttc ttttaataat tgggcttttt gtggttgaag attcatcaaa
    76261 gcagtaattt ctttttgact tttattagtt gtgtattcct ctaatgcacc gcttaaacaa
    76321 aagatgaatg tcaacattgc tccttcaaag taattaccga tcaaacaggc accaatagca
    76381 gctaaagcca tcaataaatc cacattcagt gtccgatcat tccacaattc tttcaagcct
    76441 tccgatgttt gtttatatcc accgatgata atggcactga tataaaaaag tggggtgatg
    76501 gtaagagaga atcggtcgaa gataaaaccg aagatcataa ataaaaggca gccgattgtt
    76561 gctgcaaaag cttttttctg attagtcatc gttattagac ctccttgtca aaacaaggat
    76621 agcataataa aaatgtaatt gataataata acagctaaat gagaacgata atggttatca
    76681 tttggtgatt aagcttgatg ttgtttttat tatttataat gattattatt aaagaaaata
    76741 aaaaaccaga acatttctca aataagagaa gtgttctggt tttttctaca ttgcaataaa
    76801 ccaataacca atcaaaatga taccaaggat aatacgatac cagccaaaag cggtaaagtc
    76861 gtttttcttc aaatagctta gtaagaattt gatagctaag acggaaacca caaaagatac
    76921 gaatgttcct acaagaagaa caacagtttc ttccattttg aatgaattcc cttcagctaa
    76981 atatttgacg atcttcaagc cgcttgcgcc aaacatgaca ggaatcccta agaaaaatga
    77041 aaactctgta gcgacaaaac gcgaagcacc aatcaagata gatcctagga tcgttgcgcc
    77101 agaacgggat gttccgggaa caagtgccaa gacttggaat aacccaacaa gaagagctgc
    77161 ttggtacgta aagtcattca gagaagtcca acgaggtgtt ttgtttttgt tgcgtttttc
    77221 gacaataata aaggcaatac cgtaaacgat caaaacaatg gcaactggta agaacttgtg
    77281 gaaatttgct tctaaccaat catccaaagg caaaccaatg atgatggctg gtatgcaagc
    77341 aacgatcact ttaaaccaaa gtgtccacgt atcttttttt tctaccgttg atttacttgg
    77401 agaaaatggg tttaacttgt gaaaataaag aacgacgaca gccaagatcg cccctaattg
    77461 gataacgaca ttgaacatcg acataaattg tgaactggca tccagtttga tgaattcatc
    77521 tgctaggatc aaatgaccag tactactgat gggcacccat tccgttattc cttcaataat
    77581 accgagaatc actgctttga taatatttgc aaaaaacata aattccttcc ttcccaatta
    77641 agatacatct tatagtatac cgttattttt tgagaaaccc aatctgtttt ttaatattaa
    77701 gaaaaacttg agaaataaag cagataagta cacgaagaga aaaaagaaga caaagccctc
    77761 gttcagactt tgccttttat gattctctgg taaaaattaa aatatcccca ttatagaaac
    77821 gagtcgcgac tatgtgtttt cccgttatcg atataagacg tatcattgat gccgtggata
    77881 gaaaatcgac cgtttgcata agtgaccttt gttacgctgc catttaaaag cccagcacga
    77941 acaggctgtt taggatcgat cagattaagc aaaaaggcga tcgtcaaacc atgagatacg
    78001 actaaaacat ttccaccacc atttttttcg cagtgatggg cgatatcttc aaatcctgtc
    78061 catacacggt ctcgcaatgt ttcataagtt tccgcccacc cagcggtatc taagttgtat
    78121 acagcattag caatttgttc aaaagaaact tctgtggtaa acatttcatc atatgtttta
    78181 aaattcaata cacgagggat cacaccccac atttccatgt catatccacc ttcaagagac
    78241 ccgaaacacc attctctaat ccttttttca ataaaataag gaatttcttt tcccttagaa
    78301 tgttctccta gaataatacg ggcagtttcg attgcacgac cactgtcact agaataagca
    78361 aatgaaaagt cgacattttt taacccgcga cctaaataat gaatgccttc ttgtccttgc
    78421 tttgtgaggg gtgtatcaca ccagccttgt actcgttcaa ttgtattgaa catcgttttt
    78481 ccgtgtcgta cgatataaag tgtagtttct gtcattttat tctcctccta agtcgtttta
    78541 acgattgaaa aaaggatttg tccttttttc ctgttcaatc gtcgttgcat cgccatgtcc
    78601 tggatagact gcaaattcat caggtaatac aaacagctgg gtccgaatac tatgaagtaa
    78661 ttgttctaaa ttccctgtat acagatcggt tctaccgata cttcctctaa acagggcgtc
    78721 gccagaaacg acaaaatcat caaaaataaa gctgacgctt cctatagaat gccctggggt
    78781 agggacgacg cgaaaagaca tgtttcctaa agtataagtt ttcatttcaa attcatattc
    78841 tgcaggcgag acaataacat cagcaatatc atcatgtcta ccgagtccag aaagattcaa
    78901 aatcggatca cctagccatt tttgctctag tgggctgaca taaactggga tttgataata
    78961 cgcgcgtaaa ggttccactg caccgatatg atcgtagtga gtatgggtaa gcaaaatcgc
    79021 taccggctgt tggtttgttt ttttgatttc ttgtttgatt cgttccgcat cttctccagg
    79081 gtcaatgatc aaaagtgctt cttcgttgta gaccaaataa cagttttctt caatcattcc
    79141 tgtttttaat ctttcgatat gcatgtgcat tcctccttga tagccaaaac ttctttcatc
    79201 cattagtata acgcattcat tgctaggaaa caaaaacagg tacttatttc tctttactta
    79261 tctgaattat cagactatat gaaaagtatt tggatattct aaagagaagg aaagtaagtt
    79321 ttcatagcat tttaatatgt ccttgttaca ttagattcat ccaaaaatcc aaattctata
    79381 tcctatatcc ctgcctaatc agcagggtgt ttttttatgt ttggaaagag agctcttttt
    79441 gctaaactag gttttttcag gtaatcttag agagaaagag gtggtggaaa tgatcaaaca
    79501 gtcaaaaata tctgtgcggc acttagtaga atttattttg cgacgtggaa gtatcgacaa
    79561 tcgtaaaaaa agtaaccata ctgcattaga aggagcgaga atccaccgca agctccaaaa
    79621 agatgcggga gaaaactatg aaaaagaagt ctttttaaaa acaacagttc aattagatga
    79681 atattacctg atcgttgaag gccgtgcaga cggtattttc aaaagagacg gtatttatta
    79741 tatagatgaa atcaaaacct ccgaaccacg atttgaagat ttagaaccag aacaagtgga
    79801 acttttcttt catcaagcaa gggtgtatgc ctatatctat agtcaagaac atgatttaca
    79861 agaaataaat ctgcaattga cttacttcca gacggcagaa gaagtgatca cgagaaaggt
    79921 tgaacatcag acaagagagc agttagaaac tttttttaaa aagctgactg aagattataa
    79981 agaatggctt gtttttcaag aaaactggcg cactgtacgc aatgcctctt tgatggcttt
    80041 gaaattcccc catgatgagt ataggaaagg tcagcgtgaa ctagcggtag ctgcttataa
    80101 aacgatccgt acgaaacaga aattgtttgt agaagcacct accggaacag gaaaaacaat
    80161 ctctacgtta ttccccgctt tgaaagctgt aggggaagaa gagggagaaa agatctttta
    80221 tctaacggct aaaacaataa ctagacaagt agctgaagat gctatgacag cattaaaaga
    80281 tacaggagct gaagtaaaaa gtgtcacttt gacggcgaaa gacaagatct gctttttaga
    80341 tgagacgacc tgtaatccag atcagtgccc atatgccaat ggttattaca atcggattaa
    80401 tgaaggttta tgggatcttt tgaatcatga gaatcagatc actcgtgaag tcatcgaaac
    80461 ttatgcaaga aagcatacac tttgtccttt cgaactatca ttagatgtca gtatctggtg
    80521 tgacgtaatc attggggatt acaattattt atttgatcca actgtttatt taagacgttt
    80581 ttttgaagat gaaaaaaacg aggactatct gtttttgatt gacgaagcac ataatttggt
    80641 gaatcgttcc cgagagatgt attcggcaga actatcttat gaaaaaacga aacgaagtaa
    80701 ggaagctata ccaaaggaat tcaaaaaact tcatcgccgt ttcaacaaac tattaaaaga
    80761 atttgattcg atccgagaaa tagcaaaaga agatcattgg gattatcatc atcaaaaagc
    80821 accggctgaa tctttagtaa aggcaggtta tcaattaagc gaaaaaatta aagaatggct
    80881 ggcagaattt cctgagcacc catcacaaga acaattgctt ccttattatt ttgatttgct
    80941 tcattttttg aaggtcagtg aattttatga tgatcactac gaaacaacag tcgaaaaaac
    81001 gtatcgcgat ctgatagtaa aagagttttg tattgatcca agcttgtttc ttgagcagtc
    81061 gttggataaa ggaaaaagca gtattctttt ttctgctagt ttttcaccac tctcttatta
    81121 tcaggaaaca ctggggggac aaaaaagtct ggcttacaaa ctgccgagtc ctttcccaga
    81181 aggaaaccag caagttttca tcgcaaatta cctagagacg acttatcgaa aaagggaaca
    81241 cagtctggca aaattagtag agacgattca tgcttttgct gatggaaagg tcgggaatta
    81301 tttcgttttc tttccttctt atcaatattt agatcttgcg gtggaagcat tcagacagca
    81361 atatcccgac agcaatgtgc taatccaaga aacggacatg aatgaggaag aacgcgaacg
    81421 atttttaaca aaatttcaaa caaatcccgc tgaaacattg attggatttt gcgtacttgg
    81481 cgggattttt tcagaaggaa tcgatttgag aggggaccga ttgatcggca gtatgatcgt
    81541 aggtgtaggt ttgccgcaga tgaatcatga gcaggaactg atcaaatcat attatgatga
    81601 gaaggaacac cttggatttg cttacgctta tcagcttcct ggaatgaaca aagtcttgca
    81661 agctggtggt cgagtgattc gtggcatggc ggatcaagga atcgttgtat tggcagatca
    81721 acgattttct agtttttctt atcgacgttt attcccacaa cattggcaaa cagcaagaga
    81781 agtacgaagt gttggggaat tggaagaagg aatacagcgt ttttggttat caaaagaagc
    81841 agtaaatcaa aaaaacgagg aatgacagac gtcagcctcg ttttttgatt aacgatagtt
    81901 agtgaattgc aactcgattg gtaagtcaag tttacgtaat aaagcaatca cagcttgtag
    81961 atcatcacgg ttcttacctg tcactctaat ctgatcttct tggatttgtg cttttacttt
    82021 cagtttttca tttttgatag ctgtagtgat tttcttcgcg ttttcacgat cgattccgtt
    82081 gaccaaatcg gcagtttgtc gagctttgcc acctagagcg tgttctgatt cggaaaaatg
    82141 gatatttttg attggcacat ttcttttgat cagcttacca aataatacat ctttgatctg
    82201 ttcgattttg aaatcatcat cgctcaatac aactagattg ctgttctcta atttgatttc
    82261 tactgtagaa tccttgaagt caaagcgatt ggacaattct tttgttgttt gctgaatcgc
    82321 attttttact tcttctgtat tgacttctga tacaatgtcg aagctagcat cttttgccat
    82381 aggaaccctc ctgatataca tataaatatt taccatacat ctatcttatc ataggattag
    82441 ggcagattct tacctgtttg ctaaaattct tctcatttga gttcgaagta ttttatttta
    82501 atagataaac tgttattctt acatttggaa taccaaagta ggggagaggt gcttgtgagt
    82561 aaaattatga ctgcgtatca taaaaatgaa agattaaaga aaatagtggt tattttgata
    82621 accggattga ccgctgcggt cgctttaaat tattttttga ttccagccaa tgtcttttct
    82681 gcagggatga atgggatcgc tcaaatcatt tcctcgcttt tatatgaatg gtttcatatc
    82741 aagatagaca caggaacatt tatttttctt ttgaatatcc ctgtgtttgt attagggttt
    82801 ctcaaagtag gaaaacgagc aacgatcctt agttttgtga atgttgtatg tgtatcggtg
    82861 atgacgatgc tgcttccagt cggacagatc actgataata ttttgatgaa tgccctagta
    82921 ggcggtgttt tattaggtct tggcgtagga ttatctttaa aaatgggctt tacaactggt
    82981 gggatggata tcatctctct tgtactttca caaacaactg gaaaaacagt cggaaactac
    83041 atgctgattc taaatggtat cattgtagtc atcgcaggct tcttctttaa ctgggaaagt
    83101 gccttgtata cgattatttc catttatgca atgactcaag taattgatgc catccacacg
    83161 agtcatcaaa aagtaacagc aatgatcgtt accgtcaagc cagaagaggt aacagaagaa
    83221 ctagcaaaac ggatggtacg tggaatgaca ttactgccgt ctgtcggtgg ctattcaaaa
    83281 aaacaaggga acatgatcat gatggtcatc acccgctacg aaatgtatga tcttgatcag
    83341 atcgttcatg aaatcgacga cgatgcattt atcaatattt taccaactca ttcagtattt
    83401 ggccggtttg caaatgaaaa cgaacaaaaa atgtatcgaa caactggcgt attgccagaa
    83461 ataaaaaatc gttcacaaaa aaaatgagga atcaggttta ttttagaata aatttaatct
    83521 attacatcta ataagaacat ttttataaat ttaaacgaga atcttacttc taaattctca
    83581 tttacaagca gctaaaaaaa gagtatgata aataaaaatc gtgttaagga gtgtcgtacg
    83641 atgagcgaag caaaccaaga acggtctgga caagaaatag aactaatcaa agaacgtatt
    83701 cttgctgcct tggaaatggt gattgatcct gaattaggaa tcgacatcgt caatttaggc
    83761 ttgatttatg atatagaatt caatcctgaa aatggagaaa cagtcattaa gatgactcta
    83821 acaactatgg gctgtccatt agcagatatt ttaacagaat ctatccatga cgcattaaaa
    83881 gaagtgccag aagtaacgaa agcagaagtc aaactagtct ggtatccagc atggacgaca
    83941 gataagatga gccgttatgc acggattgcg ttggggatac gatagaaatc ttggctgact
    84001 agctatgaga agcaaatagg aaaaacaaaa aagcctttta tcctttctag aagaaaagag
    84061 ataaaaggtt tctttgttta taaaattttg tctgctatat tataagttaa ttaa
//
LOCUS       DANJMA010000007        76546 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000007 DANJMA010000000
VERSION     DANJMA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..420
                     /locus_tag="PWU31_000567"
     CDS             <1..420
                     /locus_tag="PWU31_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL1083137.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="PWU31_000568"
     CDS             complement(504..1226)
                     /locus_tag="PWU31_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1083138.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="PWU31_000569"
     CDS             complement(1216..1953)
                     /locus_tag="PWU31_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083139.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLPGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="PWU31_000570"
     CDS             complement(1965..2957)
                     /locus_tag="PWU31_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1083140.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="PWU31_000571"
     CDS             complement(2972..3715)
                     /locus_tag="PWU31_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083141.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="PWU31_000572"
     CDS             complement(3956..5278)
                     /locus_tag="PWU31_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1083142.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="PWU31_000573"
     CDS             complement(5265..6230)
                     /locus_tag="PWU31_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083143.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="PWU31_000574"
     CDS             complement(6227..7765)
                     /locus_tag="PWU31_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL1083144.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="PWU31_000575"
     CDS             complement(8181..8468)
                     /locus_tag="PWU31_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083145.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="PWU31_000576"
     CDS             8602..9270
                     /locus_tag="PWU31_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1083146.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="PWU31_000577"
     CDS             complement(9561..10301)
                     /locus_tag="PWU31_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083147.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="PWU31_000578"
     CDS             complement(10431..11378)
                     /locus_tag="PWU31_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1083148.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="PWU31_000579"
     CDS             complement(11507..12271)
                     /locus_tag="PWU31_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1083149.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="PWU31_000580"
     CDS             complement(12275..12664)
                     /locus_tag="PWU31_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083150.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="PWU31_000581"
     CDS             12799..13341
                     /locus_tag="PWU31_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL1083151.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="PWU31_000582"
     CDS             13489..14121
                     /locus_tag="PWU31_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083152.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="PWU31_000583"
     CDS             complement(14255..14380)
                     /locus_tag="PWU31_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083153.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="PWU31_000584"
     CDS             complement(14514..15407)
                     /locus_tag="PWU31_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL1083154.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="PWU31_000585"
     CDS             complement(15589..16515)
                     /locus_tag="PWU31_000585"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL1083155.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWU31_000586"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWU31_000586"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL1083156.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWU31_000587"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWU31_000587"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL1083157.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWU31_000588"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWU31_000588"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL1083158.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWU31_000589"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWU31_000589"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL1083159.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="PWU31_000590"
     CDS             complement(24786..25220)
                     /locus_tag="PWU31_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL1083160.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="PWU31_000591"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="PWU31_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL1083161.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="PWU31_000592"
     CDS             complement(26117..26992)
                     /locus_tag="PWU31_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL1083162.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWU31_000593"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWU31_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL1083163.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWU31_000594"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWU31_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL1083164.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWU31_000595"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWU31_000595"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL1083165.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWU31_000596"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWU31_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL1083166.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PWU31_000597"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PWU31_000597"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL1083167.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PWU31_000598"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PWU31_000598"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL1083168.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PWU31_000599"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PWU31_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL1083169.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="PWU31_000600"
     CDS             complement(35443..36210)
                     /locus_tag="PWU31_000600"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL1083170.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PWU31_000601"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PWU31_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL1083171.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="PWU31_000602"
     CDS             37323..37721
                     /locus_tag="PWU31_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1083172.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            38000..38500
                     /locus_tag="PWU31_000603"
     CDS             38000..38500
                     /locus_tag="PWU31_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL1083173.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PWU31_000604"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PWU31_000604"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1083174.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="PWU31_000605"
     CDS             complement(39286..39513)
                     /locus_tag="PWU31_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL1083175.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PWU31_000606"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PWU31_000606"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1083176.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="PWU31_000607"
     CDS             complement(40048..40761)
                     /locus_tag="PWU31_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL1083177.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="PWU31_000608"
     CDS             complement(40764..41660)
                     /locus_tag="PWU31_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1083178.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="PWU31_000609"
     CDS             complement(41818..42660)
                     /locus_tag="PWU31_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1083179.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="PWU31_000610"
     CDS             42792..43445
                     /locus_tag="PWU31_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL1083180.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="PWU31_000611"
     CDS             complement(43508..44035)
                     /locus_tag="PWU31_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1083181.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="PWU31_000612"
     CDS             complement(44055..45002)
                     /locus_tag="PWU31_000612"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL1083182.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="PWU31_000613"
     CDS             complement(45014..46900)
                     /locus_tag="PWU31_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1083183.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="PWU31_000614"
     CDS             complement(47060..49138)
                     /locus_tag="PWU31_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL1083184.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="PWU31_000615"
     CDS             complement(49151..49426)
                     /locus_tag="PWU31_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083185.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="PWU31_000616"
     CDS             complement(49788..50243)
                     /locus_tag="PWU31_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083186.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="PWU31_000617"
     CDS             complement(50397..51605)
                     /locus_tag="PWU31_000617"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL1083187.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="PWU31_000618"
     CDS             complement(51717..52049)
                     /locus_tag="PWU31_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL1083188.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="PWU31_000619"
     CDS             52197..53072
                     /locus_tag="PWU31_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1083189.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="PWU31_000620"
     CDS             complement(53154..53735)
                     /locus_tag="PWU31_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL1083190.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="PWU31_000621"
     CDS             complement(53741..55000)
                     /locus_tag="PWU31_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1083191.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="PWU31_000622"
     CDS             complement(55002..56048)
                     /locus_tag="PWU31_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083192.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="PWU31_000623"
     CDS             complement(56283..56558)
                     /locus_tag="PWU31_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL1083193.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="PWU31_000624"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="PWU31_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL1083194.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PWU31_000625"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PWU31_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL1083195.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="PWU31_000626"
     CDS             complement(59657..60337)
                     /locus_tag="PWU31_000626"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL1083196.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="PWU31_000627"
     CDS             complement(60386..61021)
                     /locus_tag="PWU31_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1083197.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="PWU31_000628"
     CDS             complement(61087..62490)
                     /locus_tag="PWU31_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL1083198.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="PWU31_000629"
     CDS             complement(62477..63508)
                     /locus_tag="PWU31_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083199.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="PWU31_000630"
     CDS             63539..63760
                     /locus_tag="PWU31_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL1083200.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="PWU31_000631"
     CDS             complement(63925..64662)
                     /locus_tag="PWU31_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL1083201.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="PWU31_000632"
     CDS             complement(64815..66155)
                     /locus_tag="PWU31_000632"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL1083202.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="PWU31_000633"
     CDS             complement(66392..67915)
                     /locus_tag="PWU31_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL1083203.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="PWU31_000634"
     CDS             complement(68293..68904)
                     /locus_tag="PWU31_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1083204.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="PWU31_000635"
     CDS             complement(69234..69950)
                     /locus_tag="PWU31_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL1083205.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PWU31_000636"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PWU31_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL1083206.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71306)
                     /locus_tag="PWU31_000637"
     CDS             complement(70509..71306)
                     /locus_tag="PWU31_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL1083207.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="PWU31_000638"
     CDS             complement(71276..71647)
                     /locus_tag="PWU31_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1083208.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PWU31_000639"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PWU31_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL1083209.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="PWU31_000640"
     CDS             complement(72649..73122)
                     /locus_tag="PWU31_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1083210.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="PWU31_000641"
     CDS             complement(73227..74090)
                     /locus_tag="PWU31_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1083211.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="PWU31_000642"
     CDS             complement(74286..76385)
                     /locus_tag="PWU31_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL1083212.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        20972 a        16108 c        13046 g        26420 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccaggt aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccacttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt acatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaaccgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag cttttt
//
LOCUS       DANJMA010000008        71202 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000008 DANJMA010000000
VERSION     DANJMA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            191..421
                     /locus_tag="PWU31_000643"
     CDS             191..421
                     /locus_tag="PWU31_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL1083213.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="PWU31_000644"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="PWU31_000644"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1083214.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="PWU31_000645"
     CDS             1832..2008
                     /locus_tag="PWU31_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1083215.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="PWU31_000646"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="PWU31_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL1083216.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="PWU31_000647"
     CDS             2624..3091
                     /locus_tag="PWU31_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083217.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="PWU31_000648"
     CDS             3176..4060
                     /locus_tag="PWU31_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1083218.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="PWU31_000649"
     CDS             4174..4410
                     /locus_tag="PWU31_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL1083219.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="PWU31_000650"
     tRNA            4498..4578
                     /locus_tag="PWU31_000650"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="PWU31_000651"
     tRNA            4585..4656
                     /locus_tag="PWU31_000651"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="PWU31_000652"
     CDS             4781..4951
                     /locus_tag="PWU31_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083220.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="PWU31_000653"
     CDS             5171..5878
                     /locus_tag="PWU31_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1083221.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="PWU31_000654"
     CDS             complement(5937..6581)
                     /locus_tag="PWU31_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL1083222.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="PWU31_000655"
     CDS             6707..6994
                     /locus_tag="PWU31_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083223.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="PWU31_000656"
     CDS             7102..7422
                     /locus_tag="PWU31_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1083224.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="PWU31_000657"
     CDS             7503..8816
                     /locus_tag="PWU31_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1083225.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="PWU31_000658"
     CDS             8813..9532
                     /locus_tag="PWU31_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL1083226.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="PWU31_000659"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="PWU31_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL1083227.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="PWU31_000660"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="PWU31_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL1083228.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="PWU31_000661"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="PWU31_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL1083229.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="PWU31_000662"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="PWU31_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL1083230.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14969..15862
                     /locus_tag="PWU31_000663"
     CDS             14969..15862
                     /locus_tag="PWU31_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083231.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="PWU31_000664"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="PWU31_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL1083232.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17799..18656
                     /locus_tag="PWU31_000665"
     CDS             17799..18656
                     /locus_tag="PWU31_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL1083233.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18771..19691
                     /gene="pstC"
                     /locus_tag="PWU31_000666"
     CDS             18771..19691
                     /gene="pstC"
                     /locus_tag="PWU31_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL1083234.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19691..20575
                     /gene="pstA"
                     /locus_tag="PWU31_000667"
     CDS             19691..20575
                     /gene="pstA"
                     /locus_tag="PWU31_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL1083235.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20590..21396
                     /gene="pstB"
                     /locus_tag="PWU31_000668"
     CDS             20590..21396
                     /gene="pstB"
                     /locus_tag="PWU31_000668"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL1083236.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21412..22170
                     /locus_tag="PWU31_000669"
     CDS             21412..22170
                     /locus_tag="PWU31_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL1083237.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22183..22860
                     /gene="phoU"
                     /locus_tag="PWU31_000670"
     CDS             22183..22860
                     /gene="phoU"
                     /locus_tag="PWU31_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL1083238.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23098..24129
                     /locus_tag="PWU31_000671"
     CDS             23098..24129
                     /locus_tag="PWU31_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL1083239.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24126..24893
                     /locus_tag="PWU31_000672"
     CDS             24126..24893
                     /locus_tag="PWU31_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL1083240.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24993..26561
                     /gene="liaX"
                     /locus_tag="PWU31_000673"
     CDS             24993..26561
                     /gene="liaX"
                     /locus_tag="PWU31_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL1083241.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26858..27178
                     /locus_tag="PWU31_000674"
     CDS             26858..27178
                     /locus_tag="PWU31_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL1083242.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27178..27528
                     /locus_tag="PWU31_000675"
     CDS             27178..27528
                     /locus_tag="PWU31_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL1083243.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27695..28633
                     /locus_tag="PWU31_000676"
     CDS             27695..28633
                     /locus_tag="PWU31_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL1083244.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28648..29478
                     /locus_tag="PWU31_000677"
     CDS             28648..29478
                     /locus_tag="PWU31_000677"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL1083245.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29522..30547
                     /locus_tag="PWU31_000678"
     CDS             29522..30547
                     /locus_tag="PWU31_000678"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1083246.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30562..31500
                     /gene="galU"
                     /locus_tag="PWU31_000679"
     CDS             30562..31500
                     /gene="galU"
                     /locus_tag="PWU31_000679"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1083247.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31625..32065
                     /locus_tag="PWU31_000680"
     CDS             31625..32065
                     /locus_tag="PWU31_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL1083248.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32068..32670
                     /locus_tag="PWU31_000681"
     CDS             32068..32670
                     /locus_tag="PWU31_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL1083249.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32729..33835)
                     /locus_tag="PWU31_000682"
     CDS             complement(32729..33835)
                     /locus_tag="PWU31_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL1083250.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34045..35046
                     /gene="ccpA"
                     /locus_tag="PWU31_000683"
     CDS             34045..35046
                     /gene="ccpA"
                     /locus_tag="PWU31_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL1083251.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35383..37728)
                     /locus_tag="PWU31_000684"
     CDS             complement(35383..37728)
                     /locus_tag="PWU31_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL1083252.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38007..38909
                     /locus_tag="PWU31_000685"
     CDS             38007..38909
                     /locus_tag="PWU31_000685"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL1083253.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39122..40897
                     /locus_tag="PWU31_000686"
     CDS             39122..40897
                     /locus_tag="PWU31_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL1083254.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40963..42549)
                     /locus_tag="PWU31_000687"
     CDS             complement(40963..42549)
                     /locus_tag="PWU31_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL1083255.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42800..43024
                     /locus_tag="PWU31_000688"
     CDS             42800..43024
                     /locus_tag="PWU31_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL1083256.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43165..44916
                     /locus_tag="PWU31_000689"
     CDS             43165..44916
                     /locus_tag="PWU31_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1083257.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44916..46700
                     /locus_tag="PWU31_000690"
     CDS             44916..46700
                     /locus_tag="PWU31_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083258.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47026..47877
                     /locus_tag="PWU31_000691"
     CDS             47026..47877
                     /locus_tag="PWU31_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1083259.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47973..49853
                     /locus_tag="PWU31_000692"
     CDS             47973..49853
                     /locus_tag="PWU31_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1083260.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49893..50795)
                     /locus_tag="PWU31_000693"
     CDS             complement(49893..50795)
                     /locus_tag="PWU31_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1083261.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51026..52429
                     /locus_tag="PWU31_000694"
     CDS             51026..52429
                     /locus_tag="PWU31_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1083262.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52429..53862
                     /locus_tag="PWU31_000695"
     CDS             52429..53862
                     /locus_tag="PWU31_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1083263.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            54007..54933
                     /gene="trxB"
                     /locus_tag="PWU31_000696"
     CDS             54007..54933
                     /gene="trxB"
                     /locus_tag="PWU31_000696"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL1083264.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55147..56874
                     /locus_tag="PWU31_000697"
     CDS             55147..56874
                     /locus_tag="PWU31_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL1083265.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56889..57239
                     /locus_tag="PWU31_000698"
     CDS             56889..57239
                     /locus_tag="PWU31_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1083266.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57331..58191)
                     /locus_tag="PWU31_000699"
     CDS             complement(57331..58191)
                     /locus_tag="PWU31_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL1083267.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58283..58711
                     /locus_tag="PWU31_000700"
     CDS             58283..58711
                     /locus_tag="PWU31_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1083268.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58873..59049
                     /locus_tag="PWU31_000701"
     CDS             58873..59049
                     /locus_tag="PWU31_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL1083269.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59076..59522
                     /locus_tag="PWU31_000702"
     CDS             59076..59522
                     /locus_tag="PWU31_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL1083270.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59676..61055)
                     /gene="brnQ"
                     /locus_tag="PWU31_000703"
     CDS             complement(59676..61055)
                     /gene="brnQ"
                     /locus_tag="PWU31_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1083271.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            61761..62732
                     /locus_tag="PWU31_000704"
     CDS             61761..62732
                     /locus_tag="PWU31_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL1083272.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62756..64948
                     /locus_tag="PWU31_000705"
     CDS             62756..64948
                     /locus_tag="PWU31_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL1083273.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64965..65441
                     /gene="ybeY"
                     /locus_tag="PWU31_000706"
     CDS             64965..65441
                     /gene="ybeY"
                     /locus_tag="PWU31_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL1083274.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65425..65820
                     /locus_tag="PWU31_000707"
     CDS             65425..65820
                     /locus_tag="PWU31_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL1083275.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65835..66734
                     /gene="era"
                     /locus_tag="PWU31_000708"
     CDS             65835..66734
                     /gene="era"
                     /locus_tag="PWU31_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL1083276.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66877..67689
                     /gene="recO"
                     /locus_tag="PWU31_000709"
     CDS             66877..67689
                     /gene="recO"
                     /locus_tag="PWU31_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL1083277.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68073..68990
                     /gene="glyQ"
                     /locus_tag="PWU31_000710"
     CDS             68073..68990
                     /gene="glyQ"
                     /locus_tag="PWU31_000710"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL1083278.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68992..71067
                     /locus_tag="PWU31_000711"
     CDS             68992..71067
                     /locus_tag="PWU31_000711"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL1083279.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23280 a        12467 c        15244 g        20211 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact
    17581 ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag
    17641 agacacattt caaagccagt atagcataga aacttttaat gagatagttt tttttacaat
    17701 tatgagaatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg
    17761 ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt aaattattct
    17821 taccaataat aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag
    17881 aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa
    17941 cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg
    18001 gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag
    18061 aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca
    18121 ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac
    18181 tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta
    18241 tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg
    18301 gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg
    18361 ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata
    18421 cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga
    18481 tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt
    18541 tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc
    18601 ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt
    18661 tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc
    18721 aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg
    18781 tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca
    18841 tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag
    18901 ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg
    18961 gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc
    19021 ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc acgccatttg
    19081 ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac
    19141 agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat
    19201 cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt atcttagctg
    19261 gtacatttgt gttgttcgtt atgatcttgc cgactatcac aacgatgact gttgatgcat
    19321 taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc
    19381 aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt
    19441 ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag
    19501 cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc ttaacaatgg
    19561 gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga
    19621 tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag
    19681 agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatatagt
    19741 ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat
    19801 accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg
    19861 aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt
    19921 tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctaac
    19981 agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt
    20041 attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc
    20101 attggctttg actgtattca acttgcctct tttgactaga acagttgagg agtctttaca
    20161 agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac gctgggaaac
    20221 agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag
    20281 ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc
    20341 tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg
    20401 tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga
    20461 tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt tactgttcaa
    20521 cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga
    20581 agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag
    20641 aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc
    20701 aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc
    20761 tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg
    20821 aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt
    20881 tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg
    20941 atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta
    21001 gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc
    21061 ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt
    21121 gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct
    21181 atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc
    21241 atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc ctttttctat
    21301 ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag
    21361 gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa
    21421 gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg
    21481 atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcgga
    21541 aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc
    21601 accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa
    21661 ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat
    21721 gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa
    21781 gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat
    21841 aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg
    21901 gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct
    21961 actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg
    22021 acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt
    22081 cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca
    22141 gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt
    22201 gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc
    22261 gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt
    22321 atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa
    22381 atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa
    22441 gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt
    22501 cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat
    22561 tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac
    22621 gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat
    22681 gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg
    22741 aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc
    22801 gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa
    22861 actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga
    22921 aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc ctcatatttt
    22981 tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgggaaag
    23041 ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg ataaagaatg
    23101 caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt
    23161 cagcatcaag ccgctttata tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa
    23221 gaggatcatc aattgattac aggaaaactg atgaacacgg atcaaaaagg agaatcgatt
    23281 tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa
    23341 atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc
    23401 tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg tggaaaacag
    23461 ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc
    23521 atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata
    23581 gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt
    23641 tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat
    23701 tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct
    23761 gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca
    23821 gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta
    23881 tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg
    23941 ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg
    24001 ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc
    24061 ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg
    24121 aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg
    24181 gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc
    24241 tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt
    24301 tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag
    24361 cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt
    24421 ttttagcaat ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga
    24481 ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg
    24541 ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc
    24601 cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg
    24661 cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct
    24721 ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag
    24781 aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct ttaacctgcg
    24841 tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac
    24901 aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct
    24961 tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt
    25021 tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa
    25081 ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct agtcataaag
    25141 aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg
    25201 agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag
    25261 atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat
    25321 tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa
    25381 aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag
    25441 aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg aaattagaag
    25501 cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata
    25561 agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg
    25621 aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg
    25681 tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga
    25741 ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg
    25801 atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag
    25861 ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg
    25921 aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg
    25981 tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac
    26041 ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta
    26101 cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa
    26161 acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg
    26221 gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta aatggtgatg
    26281 tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa
    26341 atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg
    26401 gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa
    26461 accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa
    26521 cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg
    26581 ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg
    26641 tgccttattg ccgatagtct atctcttgaa caccgtttac caagagatta tgaacctgcc
    26701 aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg
    26761 gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg
    26821 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
    26881 atctaataaa aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat
    26941 tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc
    27001 aattatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
    27061 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca
    27121 aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg
    27181 tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt
    27241 cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct
    27301 gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc
    27361 acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt
    27421 gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg
    27481 atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg
    27541 ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt
    27601 aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga
    27661 tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca
    27721 attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt
    27781 catcgtaact ggggacatct cacgcccagg actagagttg actggttatt ttaattatta
    27841 ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat
    27901 cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat
    27961 tatttcaaga ggattaccag ctccagaaga aatgatccag gcagcaaata aaaatgggat
    28021 agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt
    28081 agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt
    28141 aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat
    28201 caaacgcggc catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt
    28261 aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg
    28321 gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca
    28381 gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga
    28441 agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac
    28501 aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat
    28561 gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc
    28621 aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag
    28681 cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgct
    28741 atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat
    28801 tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt
    28861 gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta
    28921 gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat
    28981 tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca
    29041 atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc
    29101 ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc
    29161 tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt
    29221 ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta
    29281 atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt
    29341 ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt
    29401 ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa
    29461 aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa
    29521 aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt
    29581 cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga
    29641 aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac ccgattccat
    29701 ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat
    29761 tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc
    29821 agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat
    29881 cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag
    29941 ccatgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc
    30001 aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa
    30061 tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc
    30121 tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg
    30181 attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctga cctttatcgg
    30241 attaagcgga ataggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg
    30301 cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat
    30361 ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa tgatggaaat
    30421 cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac
    30481 agccgcaaaa gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct cattaacaat
    30541 tttttgaagg agcgaatgta tatgaaagtt aaaaaagcag ttattccagc agcaggatta
    30601 ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat
    30661 aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggtatcga agacatcttg
    30721 atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta
    30781 gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca
    30841 gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt
    30901 caagcaagag cctttattgg aaatgaacca tttgtcgtta tgctaggcga tgatataatg
    30961 gaagacgaag taccattgac acagcaatta atcgatgatt acgaacgtac tcatgcttct
    31021 acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat tatcaatcca
    31081 ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca
    31141 agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc agaaattttt
    31201 gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac tgacgcaatc
    31261 gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttacgat
    31321 gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac acatccagaa
    31381 gtaggtgcac ctttacgtga gtacatcctg tctttaggta aaacattaga agcagaagga
    31441 agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa
    31501 cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt
    31561 atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt
    31621 gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg cgatacttgt
    31681 cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag tagatcagac
    31741 tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag
    31801 acaagtagaa ggcttactgg tcaaaagtaa tgaactgctc ttggatatca atggaaaagt
    31861 cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcagaact
    31921 taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa
    31981 agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca
    32041 tactgaaacg acaggaggaa aataaaaatg gctaaaaaag gtggattttt attaggtgca
    32101 gtagtcggag gcacagccgc agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa
    32161 gaacttcggg atgaattagc tgcccaagcg gatgatttga tggatatggc ttcagattac
    32221 acagatatgg cgaaagaaaa atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa
    32281 gatctttctc aacaagcagg tgatctagca ggtagtgtca aacaaaaagt aagtgaatca
    32341 gcagatgaag caggaagtga atctgacgat atcttgttaa atcttaaaga acaatcagct
    32401 gacttatcta atcgtttcaa aaaaacggca gaagatatga aaaaacctgc agatgaatta
    32461 ggaagtattg ctaaagatac ttcagaagat atcatgattg atgtaaaaga cgctgctggt
    32521 aaagcaggag atactgtcaa tgaaggaatg tcagaagcaa aaccagttgc agatgactca
    32581 aaagaaatga tgaaaatggc aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat
    32641 acaatgacag attcatctac aaatcactaa aatcacaaat ttatagaata aaaaggtggg
    32701 agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat tcttttggtg
    32761 tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc
    32821 ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta
    32881 ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac
    32941 ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt
    33001 gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt
    33061 caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa gcaattgtac
    33121 gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat
    33181 tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt gctcctgcaa
    33241 ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcaattt
    33301 ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg
    33361 caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca
    33421 gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc gtcaagatat
    33481 cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag
    33541 gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta
    33601 aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag
    33661 gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag
    33721 gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt
    33781 cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc
    33841 aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc
    33901 aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca
    33961 attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata
    34021 taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg
    34081 agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtt
    34141 aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac
    34201 gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat catccctgat
    34261 gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac
    34321 aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt caatgtattg
    34381 aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac
    34441 gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat
    34501 ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa agatgcagta
    34561 aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat
    34621 cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta
    34681 ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta
    34741 gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc
    34801 atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc
    34861 atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca
    34921 caaccattat acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa
    34981 gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca
    35041 aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa
    35101 ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg tgattcggag
    35161 ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt gttataatcc
    35221 tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg tatataaaac
    35281 aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg gactgtgaca
    35341 atactattgt cacaatccct tattactgtg tattctatta aatcaatcat ctttctcatc
    35401 tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt
    35461 atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg gtccattttt
    35521 cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat tgacagtaac
    35581 ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat cagatgataa
    35641 ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt gattgatcag
    35701 attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag tattgtcatc
    35761 gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc atgaatctcg
    35821 gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat ttccgtttaa
    35881 gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac tacgcatcat
    35941 gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc tttcgtgttt
    36001 ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt cttcatagac
    36061 gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga catctactgt
    36121 acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca tctttgccaa
    36181 gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt gatataagtg
    36241 ataagccggg atattgttgg accattctag tgactcacgg acggtttgga aggtgttcga
    36301 tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt gccaagttgt
    36361 tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg caggtccata
    36421 aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg catggttatt
    36481 ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag ttctgttatc
    36541 catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca tgtatccata
    36601 attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg aatggattgt
    36661 atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac tgaccgatga
    36721 atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc ttgttgcttc
    36781 attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt gttgtggtag
    36841 aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg taatttggcc
    36901 ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag aaagatcttc
    36961 ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg gtaatccagc
    37021 gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga ttccttttgc
    37081 agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg gtgagacatt
    37141 caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg cagaaagaat
    37201 gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct gtttgactaa
    37261 ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag atactaatgc
    37321 tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat cctctgtgct
    37381 gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg tgcgcatcag
    37441 atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg acgtttctgt
    37501 aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag
    37561 ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca aaagaagaac
    37621 agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa caaaccattt
    37681 ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc
    37741 aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct
    37801 gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga tctttattat
    37861 aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga acagggcaaa
    37921 gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt gttagtaaca
    37981 attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc atgagcggaa
    38041 agaaaatgct gttaggagca gcagcagaag cagccagtta tggatcttcg acttttatga
    38101 tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg aatattgatg
    38161 ccggaaaagc gtacatggca gaaaacggtc taacaaatat cgtcgttcat gccccttata
    38221 tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc caatttctga
    38281 gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt cacccaggtg
    38341 ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc ttgaatgaag
    38401 ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt aaaggtacgg
    38461 aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa ttaaatgaaa
    38521 aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat attaaagaag
    38581 acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg cttaaagtac
    38641 tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac gccaatattg
    38701 gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca atgtttgaca
    38761 atatttcgaa aatcttggaa actccctatg tcggcaaaga taaaaagaaa gcttacgcac
    38821 catatggaaa agaaatcgcc atgttcaaag cacaaatatt cgatccagaa gtgtttcctg
    38881 agttattagc agaagaagtg atactctaag attttctatc tgacagcagc aaaaaaggac
    38941 caggcacatt gccttgtcct tttttgttta agaaacaaag gagtactttt tgcagtcttt
    39001 ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacaaggta tactgttaga
    39061 aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa
    39121 gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata catactttat
    39181 tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta aagcaatcta
    39241 tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg ctcaaaatat
    39301 tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag gaatctccta
    39361 cagcttattt ttagtacttg cacataaact gcatctttct tatgctgttt tgttcggcat
    39421 gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgatcg tgaagaacaa
    39481 gtatcttctt ttaagtatct atttttattt tttgttttct ccgattagtt ttacacatga
    39541 atattccact cgaacatatc gtaatgccct cgtgattccg gctgttgaga tcattatcgc
    39601 ttgtttacta gctatttatt accgcaaata cggttctagc agaaaattgc tgccatggtt
    39661 tttagggctt tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt ctatctggct
    39721 tcttcctttt actgtagtag ctttgggtat cagtttcgtc cagatcttgc tagggcaaga
    39781 cttttctttc aaagaagtaa agaagttaaa aaaacgactc tttcttctct cgaaacggga
    39841 atggctgaaa ctttcattgt ttttacttcc tatagttggc ttttccatcg tctatcagtg
    39901 gatcgaatat aaaaatgaga caacatacgg tattgcaacc atcaatgacc gttccagtgg
    39961 agaatttgga cgtttgatga aacaactgat ccgaatagat gatgaaacga acttgaataa
    40021 agaaaacagt gagatatggg tctcacatgc tgcactaaat aaagcattag ccgcatctcc
    40081 tacattccat actgtttcta cccgtattga cgaattatat acgactcacg cgtggactca
    40141 aggtggaaaa gtaaaagaat tacccggaga tatcatcttt tgggcattgc gagatgtggt
    40201 aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt
    40261 caaccaagaa ttagaagaag cctatcacaa gggaaccatc aaaaagaaaa aagagttcta
    40321 tctgatggga tcaggagatg gaaagacgat gagtgatctg ccaatcgtat tcgatttcct
    40381 tcaatcagga tacgcttatg catggcgata taaagatttc catcaaggag gaaattatag
    40441 tttcggaacg caagaacaag tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga
    40501 gaattggatc gatagtaatg aaccgagaac acctaatttc caattaacaa aatcagcaaa
    40561 agtcgcgaat gtcatcataa aaatttacca agttctggct attcccgtgc tgattgcttc
    40621 aatagcaggg atattactta tgatactagg aaccgtattt gcaaaagctg atcgtgccta
    40681 ttatagttcc tctttgattg tggtcaccgg attaatactg acacaattgg tttttctttt
    40741 cggtgtctcg tggttttgtt cttatgcgcc agaaaaaaag gattattttt taagtgtcta
    40801 cactggcgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc tagttttcag
    40861 taaattaaat ctgaaaaagg ctttatcttc taaataaaaa gacccttggt agctagattt
    40921 ttaacctaac tatcaagggt ctttgtgtgg aaaaatcagt ttctattcga tttcttctac
    40981 cgtttcctgc aaacgctgtt catatttttt gtttccttta tataaatcac caatcgtcca
    41041 tgccaacagg agaaggacaa gaacaatgat tacataagcg atgtgatcag ccaatgctag
    41101 ttgagcagaa gtggcttgtt caccaaagaa actttcgatc tgtcctggta atcctcgtaa
    41161 gttcaagtaa gttagactaa tcacagagaa ccagccaaga agttttacga tgaatgaatt
    41221 tttaaatcgg ctacccatct ccgcttggct gttggtcatc atcagtaaag ggatcatcga
    41281 aaacggcaac gcaaaagcca agaacacctg ggattcattc atcaagttat tcaaagcggt
    41341 atgttcttcg atcgttcctt ttgtacttgt aaacaaaaca caaatcaata caggaatcac
    41401 agaaagcaat cgtgtgacca gtcttcttag ccaaatcggg atacgcatat ggatgaagcc
    41461 ttccatgatg acttgaccgg tcaacgtacc agtaatagta gagttctggc cagaagcaag
    41521 aagtgctaca gcaaataaag tggataagat accagatttt gctacagcaa tcaagatacc
    41581 attgctcata gctgatggat cagataaggc ttcatacaga ccaaaaaagg aaggatcttg
    41641 gacagaaccg gttttgaaga cagcgacacc catgatcaat agcagtgcat tgactagaaa
    41701 agccatcgac aattggatat tagagtccca agtagaaaaa cgtacagctt gtgctacatc
    41761 ttcttcgtct tggtgatcaa tcttccgagt ttgagaaact gctgaatgaa gatataagtt
    41821 atgcggcatg actgttgcac caataatccc taaagctcca gtcagcggag tttcgccgcc
    41881 gatgaccggt gagtctgaga atgtatgact gttagggata aagccagcaa ataaactgtg
    41941 ccaatctggg ttagacaagc caacttgata gacaaaaacg aataaaatga cgaaaattaa
    42001 gcagacaacc agtgcttcga tcttacgaaa cccgactttt gtcagtagta agagaacgaa
    42061 aacatctaat acagtaataa agacggcaat caccaatgga atatcaaaaa gtaagtagag
    42121 ggcgattgct gcaccaatga cttctgcgat gtctgttgcc ataatggcta attcagtcaa
    42181 gatccataaa atgacaccca atgtcttact tgtgcgtgct cggattgctt gggccaaatc
    42241 catttgggtc acgattccta attttgctgc catatactgt aaaagcatag caatcagact
    42301 agacatcaaa ataactgaca tcaataaata ttggaaattt tgtccaccag tgatcgaagt
    42361 ggaccagtta cctggatcca tataaccaac tgctactaat gcacctggtc cagagtaagc
    42421 aagaagtgtt ttaaaaaaac ttttcccctt tggtacatca actgttccat ttacttcagc
    42481 taaagataaa ccatttgtat gttcaataag tcgatgctta tgaaattgtt tgttggattg
    42541 attattcaag aaaaaaccac ctttcctatt tatctacgat tacattctac acctcaaaaa
    42601 gaaaaaatta aagaaaaata ttcggtatgc ctaatttatt ttcgtggcgc gtcttgttag
    42661 atgacgaaag aaattttgtt ctcctctaaa aaaaccacta ttttttgcat gaaactaaag
    42721 aatacgcctt tcttttttca ataaacttta gtaaaataag tcattgagag caattcggaa
    42781 atagaaagga gagatttata tgttatcaac aggtattgtc gtacttattg ctattattgc
    42841 tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga aagactattt
    42901 agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt caatgggaca
    42961 aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa
    43021 ataggttctg acccaacgac tttttacggg ctgatttttt cagtccgttt tttattttgt
    43081 aatttgttag actattcaga ttattcagca aaaagacaag cacagacaat cctaggctct
    43141 tgataaagac aaaggaggag taaaatgtcg atttttagaa agttaggctg gttttttcgt
    43201 caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc cttggtacag
    43261 ttagttcctc caaaagtgat tgggatcatc attgatgaga ttgccgataa aaatttatca
    43321 gtaaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttctt acaatatctt
    43381 tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga gaaagaactt
    43441 cggcagcagt tattcaaaca ttttacaaaa atggacaatc tcttttacca aaaatatcgg
    43501 actggggatt tgatggctca tgcaacgaat gatttgaatg cgatccagaa tgtagcagga
    43561 gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat cattgtgatg
    43621 attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc tttgctggcc
    43681 gtcacttcac gagtgctggg gtcgaaactc catgatgctt ttcgtgattc gcaagaagct
    43741 ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt cattaaaaca
    43801 tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga tgcaattatt
    43861 aaaaacaaac gagtcaattt tctagactcc ttatttgatc cattcattac cttgattatc
    43921 ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga aggcacgatc
    43981 tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg gccgatgttt
    44041 gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga tcgcgtcaat
    44101 gaacttttgc atgaaaaaac gcatatcgtg gaacaactgg gtgcagtgca gaccccggta
    44161 aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga atcaccagca
    44221 ttagaacaaa tcaagtttgc aatccaagaa ggagaaacat taggtattgt tgggaaaact
    44281 ggtgcgggaa aaacaacgat catcaaatta ctgatgcgtg aatacgatga ttatcaaggg
    44341 aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt attaggatca
    44401 atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga taatatccgt
    44461 ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt agcatttatc
    44521 aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga acgtggagtc
    44581 tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat caaaaatcct
    44641 gaattattaa tattagatga tgcactttct gcagtagatg ccaaaacaga agaagctatc
    44701 ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcattgcagc tcaccgttta
    44761 agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat cattgaacga
    44821 ggaacccatc atgagctgct tgcactaggc ggctggtatg aacaaatgtg ggagaaacag
    44881 caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat caatcggaat
    44941 ggacaaaatc gattccactt aaagaacaga aaaagatcat caaacgactg atgaagttcg
    45001 ccaaaccttt tagaacaacg ttcatcgttg ctatcttatt tgcgtttgct ctgtctgtga
    45061 tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg gctaaacagt
    45121 cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga gtcctagtaa
    45181 aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg aagacctatc
    45241 agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt tattttgatc
    45301 agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg ctatttgaat
    45361 tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct tcattcattg
    45421 ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg ccgattttag
    45481 gaattgtcat ctggtattac cagaaattta gttctcgtat ttaccgaaat atgcgggaaa
    45541 aattaagtca gctgaataca aaattgaatg aatcgatttc cggcatgcgt attatccagc
    45601 agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat gagtatttag
    45661 acacgcgcta tgcgatgatc aaaacgaatt cgattctgtt aagtccgatc atcaattttc
    45721 tatatgcatt ggcgattgcg ttggcgttga ctttatttgg gatcgatgcg ttgcattctc
    45781 ccgtagaagt tggactgatc tatgcattta cgacttatgt ccagggattt tttaatccaa
    45841 tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct ggtagtcgta
    45901 tcttgaagat tatggatacg gaagaatatg caccacaaca aaaccctgga gcagatcaaa
    45961 tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat ggagagcaca
    46021 atgtattaaa caatatctct tttgttgcta atccgggaga aaccgttgca ttagtgggac
    46081 acaccggaag tgggaaaagc tcgatcatca atgtgttgat gcggttttac gaattttatg
    46141 aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa gagttacgta
    46201 agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc gcaggaaata
    46261 ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct gagtttgttc
    46321 aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg atcgaacatg
    46381 gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca atcgtgactg
    46441 atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa acagaaggat
    46501 tgatccaaga aggattggcg aaaatgcgac aaggacggac aacgattgcg attgcccatc
    46561 ggctgtcaac gattcgagat gcagaattga ttttagtttt agacaaaggt catatagtag
    46621 aacgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat atgtacaaac
    46681 tgcaaagtag tggtatataa aagatgttgt gacagtagca ttttgaccta agcactagag
    46741 tgaaaataaa acatagcttc ccatattttt catctgtgag agttagtgtc gtagttacca
    46801 gatttaaaaa agacgacgtc agctagtcag aagtgcctaa tagcccctaa gaaagcgttg
    46861 acaaaatcgt ttgctttcga taatataaag aaaacgatac aacttggata gtgattgcct
    46921 gatgcaagct aaacctgatg agtctcccat ggtttcatgg acggcttttt gggtttttct
    46981 ttttgttttg gatacagccc tatatatcat ggaaaggagc aaaacatgct gattgagaaa
    47041 gtactgaata acaacgtcgt aataacaagg gatgaaagag atcgggaaat gattgtgatg
    47101 ggaaaaggat tggcattcaa aaagaaggtg ggtgacccga ttcttgactc atgcatcgac
    47161 aaaaaatttc agttgacaaa tgacggactt tcacaacagt tccaagaatt ggcacaagcg
    47221 attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctctg
    47281 caagtccatt taaatgattc tgtgtatatt tcattgacgg atcacttgta tacagctatc
    47341 gaacgtcaaa aaatggggat ctttgtgcca aatgtattgc tgtatgatat caaacaactg
    47401 tttcctaaag agtatgccat tgggaaaaaa acagtgaaga aaatctcgga taaataccac
    47461 atcaagttat cagaagacga agccggattt atcgcattgc atttagtcaa tgcacaaggc
    47521 attcaaagcc agcaagacaa tacctacctg tttaccacaa caatacagga tattttaaat
    47581 atcgtccgct attactataa gattacattt gatgaagatt cagtccactt ttttcgattc
    47641 gtcacacacc tgcgtttttt tctgacacgt gtcctagatg gagcgaaatc agaagatgga
    47701 gaagtttctg aggaattgtt ccaagttatt caaaaaaaat ataaggatac gtttggttgc
    47761 gttgagaaaa tagcagactt tttaaccggg gctttcgact accagatgtc cacggatgaa
    47821 aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaata
    47881 aaaaaggagg acaaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta
    47941 aaccttccac taattaattg gagggaatag aaatgggaaa ataccaggaa ttagcagaaa
    48001 acatcgtaaa gaatatcgga ggaaaagaaa atatccgtag cttgacacac tgtgtgacgc
    48061 gacttcgttt caaactaaag gaggaaaata aagcaaagga tgatgttttg aaaaagatgg
    48121 aagggatagt caccatcatg aaaagcggcg ggcaatatca agttgtaatc gggaatcatg
    48181 tgccagatgt ctatgcagaa gtactgacag tagccgggat ttccgatgaa gcagaagaag
    48241 aggtttcttc aagtagtaat ctgttcaatc gattaatcga catattaagt ggctgtttcc
    48301 agcctttttt aggtgctttg gctgcagcgg gaatggtcaa aggattaaat gctttgttct
    48361 tgtttctcaa attttatact gaaggatcgg gtacgtacat catgctgaat gggatagggg
    48421 atgcaatttt ttatttcatg ccagtgatct tagggtacac agcagcgaaa aaatttcatc
    48481 tgaatccgat ggtggggctc gtgattggtg cagcactttg ttatccgact gtacaaggag
    48541 cagcactaca aagtggtttc cctgatggca gtcctgcgcc ttatagcttg ctgggtcttc
    48601 cggcttatga aacatttttg ggtatcccat gggtgggcag cagttatact agtagcgtcg
    48661 tacctgttat cttcattatc gcatttgccg gacaagtcca aaaaattgcc aagaaatgga
    48721 ttcctgcagt cgtccagacc tttttggttt ctttttctgt gttattaatc gcacttcctg
    48781 ctggcttttt gattatcggt ccgattgtca gtatactgac agatctatta agcacgggat
    48841 tccaagcatt gatggacttt tctccagctg tgtatggtgc agttctaggg tttctttggc
    48901 aagtactggt gattttcgga cttcattgga gtattatccc tcttgcgatt atgcagctag
    48961 cgcaaaatgg gtggagtcag atgttagtag gcgtttatgg cgcgagtttt gcacagacag
    49021 cagtggtatt ggcgatgtat ttcaagttca aggatcaaaa aatgaaggcg ctttgcatgc
    49081 cagctgtgat ttcaggaatc ttcggcgtaa cagaaccggc tatttatgga atcaccttac
    49141 cgaagaagaa gccatttatt tactcgatga ttggtggtgc tgtttcagga atctatttga
    49201 tggcatctgg aacaatcagc tacataatgg gcggattagg gatttttggg gtggtaaact
    49261 acatcacgga aacagacgcc agtggtatgt ttcaagcgtt tatcggcatt gctattgctt
    49321 cggtaatcgg gttttcgctg acttttttct tttggaagga tgaagcaggt gaaacagatg
    49381 ctatacagaa aaaatcaggt aaagaaattg taaaaacacc gatacaagga caaatcgctc
    49441 cactaaatgc agcgaaggat gcagcttttg cacaaggaac actaggtcga ggaatcttaa
    49501 tctaccctga aaaaggagaa gtacgtgccc cttttgacgg aacagttatg accctgttcc
    49561 cgacgaaaca tgcaatcgga atggtttctg aaacagggct ggaattgctg attcatgtcg
    49621 gattggatac tgtgcagttg gaagggaaat attttgaaag cttagtgcag caaggagaaa
    49681 gagtgagaca aggcgatctg ttgctacgct tcgacaaaaa gaaaatcgaa aaggaaggat
    49741 acagtttaga aacacctgtg atcgtcacca attacacgga ttatttggat attttggaaa
    49801 accaagggga gacggtcggg ccagacgatg cacttatcac cgcactgacc tgaacggtta
    49861 ggtgaagaat ggactaggct taactattct ttttattcca cttctttatt ttcaaaattg
    49921 aaaggctctt tcatgatggc tgagctgata gggcgtttat cgaatttttg accgacagaa
    49981 attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct
    50041 agaagataac aaattgatgt attgatcgta aaattaccaa tttttgaata cagtcgctcg
    50101 cgtgtcgtaa gtcgcaaagc ggctgtactc attgcagata gcgttgaatc accgatactg
    50161 gtcaaagcta aaattggaac atcattttgt ttcaatattt ctgcggtacg aaggatcaca
    50221 ttgttttcac ccgtataaga aatcagaatc ccgcacatat cgctcgtaca attgtacgct
    50281 tcatagaatg attcaccaaa agtggtgaca attgtgacat ccattttgat gcgcttcatt
    50341 ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaagat cacgattttt
    50401 tttgcctgga gcagtaaata tttagctttt tgcagttggt catgatgaat gagagaaagt
    50461 gtatcatgaa tcgtcatctg ttctaaagaa gccattttat ttgcgattgt cataataccg
    50521 tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct
    50581 gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct
    50641 acacggatga gtgtagacgg gtggacataa catcgctcag ctatcgcttg gatggtcatt
    50701 tcgccaagtg ctggagaatc gcttaagaga aactggatca cttcattttc tgaaggagaa
    50761 aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttattttttt
    50821 acgtaaatta taactttttt gaaaatgttt tgtaaacgga tttatactat ataacttaaa
    50881 ataggctttt tttgacaaga tatttacaaa aatccactag aaaacagtaa cttttgtaaa
    50941 tgttactgga aagaaacgga tttctattgt ttgttttgtt tacaaaaaag ataatgaaag
    51001 tgtaaaaaag taaaggagtg agaacatggc aaaaggaatc aaaatcgcta cgattggtgg
    51061 aggcagcagt tacacccctg agttgatgga aggatttatc aaaagatatg aggaattacc
    51121 gatacgagaa atctggctcg tagatgtaga agaaggaaaa gaaaagcttg agatcgttag
    51181 tcagatgtct cagcgtatgt gggatgcttc tccatatgaa gtcaagatct atgcaactct
    51241 ggatcgggaa gaagcattga aagatgcgga ttttgttacg actcagtttc gtgtcggtca
    51301 attagatgca cgaatcaaag acgagcggat ccctagttat tatgggatga ttgggcaaga
    51361 aacaaatggt gctggcggga tattcaaagc ttttcgtacc gttccagtcg ttttggcaat
    51421 cgttgaggac atgaagcgtt tatgtccaaa tgcttggctg atcaatttcg cgaacccaag
    51481 tgggatggtg acagaagcag ttatcagata tggcaaatgg gataaagtga ttggattatg
    51541 caatgtgcca gtgatggcga tgatgacaga accagcaatg cttggcaaga aaaaagaaga
    51601 gctgatctac aagtttgccg gtctaaatca tttccattgg cacaaagtag cagataataa
    51661 aggaaatgac cgcacggcag aattgattga tcgtttatac gatgaagata atggattacc
    51721 gaaaaatatc tttgatatcc cgtttttcaa agaacaatta aaacaaatga acatgatccc
    51781 ttgtggctac caccgctatt actatcgtga agaagaaatg ctggaacatg ctttagaaga
    51841 atatcaaacg attggtacaa gagcgcagca agtgaaagaa acagaagcag agctatttga
    51901 attatacaaa gatccagaat tagaccataa acctgaacaa ttgcaaaaac gaggtggcgc
    51961 ttattattca gacgctgcct gtgaaacgat tgcttcaatc tatggaaata aagaaacaca
    52021 gatcgttgta tctacaaaga atgatggagc tgttcctgac ctgtcggcag attgtgttgt
    52081 agaagtcact gcctatgttg gcgcacaagg agcgagaaat gtagcgtttg gttctcttcc
    52141 tgctgctgaa agaggctggt tgcaagtaat gaagaatatg gaattgctga cgatcgaagc
    52201 agctgtgact ggtgattatg ggacagcttt acaggcgttt acgatcaatc cacttatacg
    52261 atcaggaaag acagcaaaac ggatcatgga tgagttgttt attgcacacc agcagtattt
    52321 accgcaattc aaggaaacaa tcagccgatt aaaagaagaa ggaatcgaag ttcaagatga
    52381 actggcaaga gaattatcag gtaatacaca tataatggag gaagtataat ggggttcaga
    52441 aaagattttt tatggggcgg tgctacagca gccaatcaat gtgagggcgc ttatgatgaa
    52501 ggtggacgcg gactagcaaa cgtagatgtt gtgccaatcg gaaaagaccg ttttcctgtt
    52561 atcaccggaa agaaaaaaat gtttgaattt gatgaggatc atttttatcc agcaaaaatt
    52621 gcaatcgata tgtatcatcg ttataaagaa gatattgctt tgtttgcaga aatggggttc
    52681 aagacttatc gaatgtctat cgcctggagt cgtatttttc caatggggga tgagaaagag
    52741 ccgaatgaag ctggcttgaa attttatgag gatgtcttta aagaatgcca aaagcacgga
    52801 attgagccac ttgtgacgat tacgcatttt gattgtccaa tgcatttgat caaagaatat
    52861 ggtgcgtggc gttccagaga actcgttggt ttttatgaaa atctgtgtcg agtcatcttc
    52921 aatcgctata aaggacttgt caaatattgg ctgacattca atgagatcaa tatgatcctt
    52981 catgcaccat ttatgggcgc agggctgtat tttgaagaag gagaaaatga agaacaaatc
    53041 aaatatcaag cggcacatca tgagttggtt gcaagtgcca tcgcaacgaa gattgctcat
    53101 gaagtagatc cggagaacaa agtaggctgt atgctggcag caggagcata ttatccatac
    53161 acttgcaatc ctgaagatgt ttgggcttct agaaaatcag atcgagaaaa ttatttcttc
    53221 attgatgtcc agtcacgagg agaatatccc gcttatgcat tgaaagaaat ggagcgaaat
    53281 ggcattcaga tcgaaatgga accaggggat gccgcattgc tgaaagaaca tactgttgat
    53341 tttatctctt tttcttatta ttcttcacga gtttcgacta ccgacaacga actcttggaa
    53401 cagactgcag gaaatatctt tgcttctgtc aaaaatccat atctgcaagc aagtgaatgg
    53461 gggtggcaaa ttgatccatt aggcttgaga ataacgatga atgatattta tgatcgttat
    53521 caaaaaccat tatttattgt agaaaacggc ttaggtgcag tcgatacgcc agatgaaaat
    53581 ggttatgtgg aagatgatta ccgaatcgat tacctggcag cgcatatcca ggcgatgaag
    53641 gatgcagtag aactagacgg agtcgatcta ttaggctata cgacttgggg atgtatcgat
    53701 ttagtatcag ctggtaccgg tgagatgaag aaaaggtacg gattcattta tgtcgatcgt
    53761 gataaccaag gaaatggcac attagcccgt tcaaagaaaa aatcattcaa ctggtataaa
    53821 aaagtaattg attcaaatgg agaaaatctt tcgaacaatt gatcaatgat ttataaaagt
    53881 ctattgtttt tcagcggatt tttcgagtat aataaagcta ttcgagaaac gcaagcagaa
    53941 caatagactt tttttcgcat tggctgtaaa gtatgcttac ttgattaagt aaaggagaat
    54001 ctacatatgt atgatgtaat cgttattggc gcaggtcctg ctgggatgac tgctgctttg
    54061 tatgcttcac gttcaaactt atccgtattg atgatcgaac aaggtgcccc tggcggccaa
    54121 atgaacaata cggcagaaat cgaaaattat cctggattcg attcgatcat gggccccgat
    54181 ctagcttata agatgtatga aagtgtctca agatttggga cagaaaatgc ttatggaatc
    54241 gttcaagata tcaaggatca cggcagccat aaagaagtca tctgtgaaga taaaagttac
    54301 gaaggaaaaa cagtcattat cgcaacaggc tgtatccacc gtaaattagg cgtacctggc
    54361 gaggaagaat ttgctggacg aggcgtttct tactgtgcag tctgtgatgg tgcgtttttc
    54421 cgtaacaaac gtctagtagt catcgggggt ggggactcag cagtagaaga agccatctat
    54481 ttgacacaat ttgcttctga agtagtgatc gtccaccgta gagatgaatt acgtgcacaa
    54541 aagatcatcc aagaccgtgc atttgcaaat gaaaaaattt cctttatgtg ggatacagtc
    54601 gttgacgaaa tcgttggaaa cgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa
    54661 accggcaatg aatttgattt agcagcagac ggagtattca tctatgttgg actggatcca
    54721 ttaactgcac cttttagaga agcaggtttg acgaacgaag ctggctggat tccaacagac
    54781 aacgaaatga aaacgaatat gccaggcgtt tttgccatcg gagatgttcg tgaaaaagat
    54841 ttgcgacaaa tcactacagc tgttggtgac ggcggtattg ctggacaaca agtgtataaa
    54901 tacttggagt ctttagaaga aacaacagca taagtttaaa aagagtgaaa gaacggtttc
    54961 aattgttctt ttactctttt ttttcgcgtg aaatccactg aatccgattt atgtgaatac
    55021 ttgcaatcat gcttgataaa agaatccttt cttgatttaa gcgttttttg gtcctcatac
    55081 tttcatatct aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt
    55141 attttcatgt cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa
    55201 aacttaaaaa aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt
    55261 tatgcaccgt tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat
    55321 cgaatgaaca tctatacggt aagacaagca acagaaggac tagcacgttt catggataca
    55381 caaggtgaag aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcagtca
    55441 ccagaatttg caatggaagc agccaaaaca ctggcacatc acaatatccc gtcttatgta
    55501 ttcgaaagtc tgcgtccaac accagagctt tcttttgcag ttcgtcattt gaagacgttt
    55561 gcgggaatca tggtaaccgc atctcacaac ccagcggctt acaatggata taaagtctac
    55621 ggagaagatg gcggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt
    55681 gaagtcagca atccgctaaa agtcgaagta ttatctgacg aagaagctaa acatagcggg
    55741 ttgatcacga tcatcggaga agaagtggac gctgcttatc tagaagaaat caaagcagtg
    55801 acgattgatc gtgaattggt tgaaacaatg gggaaagatt tgaaacttgt gtacacgcca
    55861 cttcatggaa cagggaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa
    55921 ttcgtactag ttcccgaaca agctgtggca gatccagatt tcactacggt caaatcgcca
    55981 aatccagaag aacattcagc ttttgaatat gctatacgac ttggagaaaa agagggagct
    56041 gacttgctga ttgcaacaga tcctgatgca gaccgtttag gcgcagcggt tcgtttgcca
    56101 gatggttctt atgaagtatt gactgggaac caaatcggag cattgtttgc tcaatatatt
    56161 ttggaagcac atcaatcaag aggcacttta ccagaaaacg cagcggtatt gaaatcaatc
    56221 gtttccagcg aattgcctac agcaattgcg gaacactacg gtgctactat gttcaatgtg
    56281 ttgacagggt ttaaattcat tgctgaaaaa atccaacaat ttgaagaaga ccattcacat
    56341 acttttatgt ttggattcga agaaagttat ggttatttag ttaaaccttt tgttcgcgat
    56401 aaagacgcga tccaagcatt gcttttatta gcagaagtag cagcttacta taaaaaacaa
    56461 ggcaaaacgt tgtatgacgg cttgcaaacg atttttgaaa cctatggcta tttcgcagaa
    56521 aaaacgattt ctgtgacaat gagcggacaa gaaggttctg cgaaaatctc tgctttgatg
    56581 gaaaaattcc gtaaagaagc accgacagaa tttgccggag caaaagttat tcaaacagaa
    56641 gactttaaag aattgacgag aacagaccgt gaaggaaacg tagaaaaact aacaacaccg
    56701 ccatccgacg tattgaagta tacattggaa gaaaatggct ggatcgctat ccgcccttca
    56761 ggaactgagc cgaaaatcaa gttttatatt ggtgtaaaag gaagctctac agaagacgca
    56821 gccgaaaaag tagcctctta tgaagcgatt atcaaaaaga tgacagaaga ataaaaaact
    56881 tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag ttggaggtga
    56941 atggattgcc agacacgcaa aaatcaaaag tagagaaagt cagtcaatta ttcaaaatgt
    57001 tgagtgaccc cactcgtctt aaaattctgc tatatctgaa agacggggaa caaaatgtga
    57061 cagcaatcac ccaagcagtg gaaatggagc agtctgctgt atcgcatcaa ctacgtttat
    57121 taagagaaaa tcatgtcgtc aagtctcatc gcgagggaaa agcgattttg tatagtcttg
    57181 atgattctca tgtactggat attttgaacc agacactcaa acatgtggaa catcaataac
    57241 gataggtgca aatagataaa cactggagtg tgacaacgaa atttcttcgt tgtcacactc
    57301 cagtgttttt taagaaaaag acaaaaatga ttaatcgctt ttttctgttg tttcagttcc
    57361 ataataacta tgatcttcta ggtctaaagc attcaaaata agcgaagccg tttcttcaat
    57421 agataaagat gccacattga tcactacaca tcctagtttt tcataaagat cattcgcaaa
    57481 atcaagttca gctctaattt tttcgatatc ggaataagca gtatcagggt ttaatccgta
    57541 agagcgcatc cgttctttac ggatattatt taaaatatct gggttattcg ttagtcccac
    57601 gattttttta ggatcgatct cccaaagctg tttgggaata tgtgcttgtg ggatcaaagg
    57661 taaattggca acttttaaat ttttgttagc taaaaacaag ctcaaaggcg ttttggaagt
    57721 tcgggatacg cctagcaaaa cgacatctgc ttctaaaaaa ccacgaggat ctttcccatc
    57781 atcgtatttt acagcaaatt ccattgcttt gatccgtttg aaatagttct cattcaataa
    57841 atggagggca ccaggttgcc gaattggctt gataccagtt aaacgttcaa tttctgatat
    57901 cggtggcgtc atcacatcaa aactaaataa ttgatgttct tcacaaaaag aacgtgttat
    57961 ctcgatcagt tcttgattga ttaatgtatg caggacaagt gctttttctt ctaatgcatc
    58021 ttgcaatgcc tgccttaatg ttttttcatc tgtcacgaac gttctgcgga ataggttgaa
    58081 ctcaacggta ggatactgag ccatagtagc ttgcgctagt tttgtcgcag tttctccagc
    58141 agagtcggag atgacaaaaa aagtaacgat ttctgtagaa tcggaagaca tggataaaac
    58201 ctcacttgca ttttttttat gtttattata ccatactcta gaatgcgtac ttcgtaatca
    58261 ttttgatttg gaggaataag tcatgaaaaa acagacattg atcgaacaat cattagaaac
    58321 attgaaaaat aacggtttta aatatactaa aaaaagagaa gcattgttaa cttatttagt
    58381 gaagcgtaac cgctacgtat ctgctaaaga agtttttgac tatatgaatc aggaattcaa
    58441 aggggtaagt tatgatacga tttaccggaa tctccatgat tttgcagaga tcgatttact
    58501 ggaagaaacc gatttaaatg gagaaatgaa gtttcgtttt cattgctgtc aggggaaaat
    58561 cggccatcac catcatttta tctgtacagt gtgcggaaca acgaaagaaa tccatatgtg
    58621 tccgatgaat tatttcgaag aacagctagg tggatgcacg atcgaagggc atagattcga
    58681 gattttcgga agatgtgaaa attgtcaata aggatttaaa taaaataaca tttgataatt
    58741 ttaaaaaaaa gcaagatttc cttgacggtc aaaacagcat tagatataat atttacagga
    58801 cagtcttgtt ttttgagata tatttcaata acaattacgg ttacttttta agctcggagg
    58861 gagggaatta acatgtcaaa aacagtcgtt cgcaaaaacg aatcacttga cgatgctctt
    58921 cgtcgcttca aacgttccgt gtcaaaagcg ggtactttac aagaatctcg taaacgtgaa
    58981 ttctacgaaa aaccaagtgt aagacgcaag aaaaaatctg aagcagctag aaaacgcaaa
    59041 aaattctagt tctgtgatga attggagttg gtagtgtgtc actactaagt agattaaacg
    59101 atgacatgaa agcagcgatg aaagcgaaag ataaggaatc tttacaagtc atccgaatga
    59161 tcaagtcatc tatacaaaac gagcagatta aagaaggtca cgacttgaca gaagaagaag
    59221 aactaacggt gttgtctcgt gaaatgaaac agcgacgtga ttctttgcat gagtttgaag
    59281 aagcaggacg tgacgattta gctgaaaaag taaaaagtga aattgtaata gtcgaaaaat
    59341 atatgccgga acaactttcc gacgaagaaa ttcgacaact tgttcaagaa gcaatcactc
    59401 aaactggtgc ttcatcaatg aaagaatttg gcaaagttat gggtgcaatt atgcctaaag
    59461 tcaaaggaaa agcagatgga aaccaagtga atgcgattgt gaaagaacta ttacaatctt
    59521 aattttatca tttaaactgc ggacagtgat tatccgcagt tttttgtgtt cgtaaaaaaa
    59581 gaactaaaaa acagctagaa gaaagaatct tattctttct tctagctgtt tttttcctgt
    59641 ttaatatctc ttatgttaaa ttgtgtttga tttggttatt cttctggtaa ctcacttgct
    59701 tcttgtttat atcctgatac gcgatataca taataaaaga gcaagctgcc agcgtaacca
    59761 agcaacgttg ggacgaccca gccaagacct aatgcagcaa aaggaacata atgatgataa
    59821 aatcctagta agcttgctac gacagtttgc tgactcacta atgcaggcga atttgccaac
    59881 atatccaata cggctggaac ggcagaaaag aacatcgtta cttgatagac aaatggactc
    59941 ttttggaaga attttgctgt taaggaaagt aagattaacg caagtgacaa tggatacaaa
    60001 agcatcaata ctggtaaaga ccattggatg atattatcca atccggcatt cgcaacgaca
    60061 aaagagacaa aagtcgttaa ccgcagccat gtcatataac tgactttagg gaacagttta
    60121 tggaaatctt gtgcaaaaga aacaacgagt cccatagcag tagtgaagac acctaatgtg
    60181 accattaccc ctaagaagct agcaccgaag ttacctaaat aaaaattagt gatctgagaa
    60241 agtgcagttc cgccattttc tgataaagac atcttattca aactcatcgc accgagaagg
    60301 accagaccaa aataaatcat gacttctaac gcaacgctca ggctaccagc ttttgctgtt
    60361 aattctgtga cttttttatc tttaaaacca agatcacgaa ctgcatggac aaaagtgaca
    60421 cttaaagcaa gtaaagctac tgcatcaacc gtgttgtatc cttctaaaac gccatttgtc
    60481 aaagcgttcg tctgataaga atctgcagca gattggttta gattccccat gggttggaat
    60541 aaagaaacga caaaaatgat agctaataat gcaagaaaca aggagtttaa atactttccg
    60601 acatatttga ccaaacgtgt tggatgtaca gtaaggaaat aagcaagtaa aaagaaaaga
    60661 gcagagaata aaaacatacc taatgaatca tatttttcag gcaaaaaggg tttagcagcc
    60721 atttcaaaag cagttgctgc tgtccgcggt gtaccaaaga atggtccgat cgttaaatga
    60781 acaaggatca agaacaaggc agcataccat tttccgacag gtttagctaa gtcatacaga
    60841 ccatcacttt ttgtcactac tacagctaag atagctaata atgggaaaag agcgcctgag
    60901 atggcaaatc ctacaccagc gctgatccag tttgatccag ccatttgacc taagtgtaca
    60961 ggaaagatca gattacctga cccgaagaac atcccgaaga taagtgaact gaccatcaat
    61021 aattgcttcc atgaataagt ttttgtttct tccataaatt tttcctccaa taattatcat
    61081 tttgagtaac aaaataaacg ttgcagaaac tagaaatacc actaatatca gattccatga
    61141 aaaccaccac cttaaaatat aaaaaaagcc cttttccact tgggaaaagg gctctagatt
    61201 ctagaacggt accacctttt ttcgcttaaa aaagcttctt cattcacgga cgcttttcga
    61261 taaagttatc cgctagcctg ataatggagg cagccacaat gatctacgtt ttcgacattg
    61321 actcggaaat gatgttcgtc tattcgattt attgtcattt ctcacttaca tgactcactg
    61381 ggaataaatc actgaatagc ctactgtttt cctcgacgct ttgctgtata tttatttgtt
    61441 gacgttataa tacacctgtt ttttttgaaa tgcaagaata tttcaaatat tttgttttct
    61501 ttttttgaaa caagaaaaat acccagagaa tacttgctaa gttagacgga atacttgtcc
    61561 tctatcttat gcatagctta catgatcagc tttttttatt caaacgagac ctttagagaa
    61621 tggaagcggc tagtttttta acgtttcttg gtacttatgg ttaagcaact cttttagaac
    61681 cttttcttat ttgctatata gtttcttctg agtatggtaa aataggaaag aatgaatcga
    61741 aaaaaagggg atgtctattt ttgacggaag agtctagttc tttagagatt agattgacgg
    61801 ctgctgatga tgtcagcatg cttttaggtg cacacgacaa gcatataaaa ctgattgagg
    61861 aaacaacgga aacaaccatc catacacgtg gagaaatcat tcagatcatt ggtgagcaat
    61921 cccagatttc cttggctgcc tcagtcattc ggacattaca ggaattgatc aaacgaggaa
    61981 tccacgtaag tacgcccgat gttgtgacgg cattgaaaat ggggcaaaaa ggtacattag
    62041 attattttgt tgagatgtat gaggaagaga tcgtcaaaga ccgtaatgga aaacctatac
    62101 gggtaaaaaa cagcggccaa aagaaatatg tcgaatcggt agccaaacat gatatcgtat
    62161 ttggcgtagg accagccggt acaggaaaaa cttttcttgc agtagtatta gcaatcgctg
    62221 ccttgaaaaa aggtgaagtg caaaaaatca ttttgacgcg gcctgctgtg gaagcaggtg
    62281 aaaatctagg ctttcttccc ggggatctaa aagaaaaagt ggatccttat ctgcggcctg
    62341 tttatgatgc tttataccaa atttttggat tggatcatac gaaccgattg atggaacgag
    62401 gcgtaatcga aatcgcacct ttagcttaca tgcgggggcg aacattggat gatgcattcg
    62461 ttatcttgga cgaagcacaa aacacgacag tcgctcagat gaaaatgttc ttgacccgtc
    62521 tcggttatca atcaaaaatg attgtcaatg gggatacaag tcagatagac ttgcctcgag
    62581 ggacgacaag tggtcttgtt catgcagaac gaacattaaa gcaaatcaag aaaatcgatt
    62641 ttgttaattt tgaagcaagt gacgttgttc ggcaccctgt agttgccgaa atcatcaaag
    62701 cttatgaaaa ggcagatctc catcaagaat aagctttgct taggaggata agcacatgtt
    62761 gaataaaaca ctgcagaaac tacaagaaaa acttggaaag gcagcattgc ctttcctgct
    62821 gttcttgttt tttatcatta tctgcggcgt tacatttagt agtgtcaaac aaaaggccaa
    62881 tgatttcaga gaaggccaag tagcagaaga aagtatccgt gcgaacaaaa cgatcgagaa
    62941 cacggaagaa accgaacaaa aaagaaaact ggcagcagaa gcagtcactc cagaatacac
    63001 ttaccaaaaa gatcttgcag atgaccagaa caatcggatc aagcaactat ttgaattgat
    63061 cgataagacg aatgatgcca tcaacaaatc ttatgacgaa aagattgata aggcaaagga
    63121 agatgaaaat atcccaaagc ctactacaga agagcggatc gccgcgctga agaaaaattt
    63181 tgaaaatgtg aatgcagaaa atgttgtgtt ttatcaaaaa ctaccgaaca atttttattc
    63241 gacgatattt gaaatgactg aaactgagat tcatactgtg agggatgaga gtctgaagct
    63301 cattgacgaa cagatgagca aacaagttcg tgaaagtgaa ttggaagctt ttaaacaaga
    63361 agcagaagag caaatccaat atttgaatgt cacaccagca caacaacaaa tgattcgcta
    63421 cttagtggat caaggtatcg tagtgaatga cgtattgaat gagaaaaaga cagaagaatt
    63481 gaaacaatcc gcacgagaag ctgtacaacc agtgatgatc ttccaaggag aaatcattgt
    63541 tcgtgaaggt aatcaaatcg atgcttcagc tatgaaaaaa ttggaattac taggactgac
    63601 taatcaaaca acttcgattt ttccgctaat tgccatggtt ttagctgttt tgcttcaaat
    63661 cggcgtactg atttataatt cacttcaatt ccaaaatgca ggagaaagaa caaaatacgt
    63721 gcttttctat gtgacagcta tgtcagtcag catagtgctg atgaaatttt tccaattatt
    63781 ccaaacggaa caagctactt atatcccgct gttttatccc gctgcttttg caccacttgt
    63841 tttaagcttt ttcttgaatc gaagagcagg gatcatggct ggtattttcc aagcagtatc
    63901 cgcacttttc attttttatg attcgattgg cacgaacttt ctaacgatta tcttgatgtc
    63961 ttatgttttc tctgggctca tggcaacgat tgtcagacgc aaacggatct cagaacaagg
    64021 attatctgct gccatgtggg tcattatttt tcctgtgatg atggatgtca ttttgatcgt
    64081 ctatcaagga atgagctttg gtgatgcaac gacttggtta atgctcgtct gcgggttcac
    64141 tggtgcactc ttctcattct tgctgacgat cggtctacac ccatatattg aattgatggt
    64201 caatgatgac agtgtaattg tattaaatga actcagcaat ccaaatcatc ctttattgaa
    64261 acagttgctg gaagaagcgc caggtactta tcatcatagt atgatggtag ctaatctaag
    64321 tgctaacgct gtagcagaaa tcggcggacg ttctttgcta actcgagtag cttgctatta
    64381 tcatgatatc ggcaagatca aacatgctag ctttttcgtt gaaaacttgc cttcaggagc
    64441 ggaaaacccg cacaatttct tattgccaga agacagtaaa catattatct ttggacatgt
    64501 gacagatgga gccaaaatat tggaagatta taatatgccg caaatggtga tcgatatttg
    64561 caaacagcat catggaacca cgctaatgcg ttatttctac atcaaagcga aagaacgcaa
    64621 tccggaagta acagaagagc aattccgtta tcctggacca aaaccgcaaa gtcgtgaagc
    64681 agggatcgtc aatattgctg acagttgtga ggctgcagta cgggcaatgg atcatccgac
    64741 gagcgaaaaa atcgaaagtt ttgtccataa tcttatcgaa gagcgtattt ccgatggtca
    64801 attagacgat agtggactga ccttgaaaga aatccgaaaa gtggaaaaat cattaatcag
    64861 cggtttaagt agcaccttcc attctcggat caaatatcct aaaatgaaaa cagaagttga
    64921 gaagataaag gaagaacaag aagaatagaa agaagcgatg tataatggat attaccttta
    64981 tggatgaaac aaaccaagtc tcagaagaga aaatgaaaga aatcgatgac ttgctgcaat
    65041 ttgcagcatc ttatttaaag ctgccagagg aaacagaaat gtctgttact ttcatggaca
    65101 atgcggcaat ccaagtaatc aatcgtgatt accgtggaaa agatgcaccg acagacgtca
    65161 tcagttttgc tctggaagaa gaaggcgaag acgaaatccc agtgattttt gaagatggag
    65221 agaatccatt acctcgtgta ctaggagaca tcatgatctc tattgaacgt gctgctgaac
    65281 aagcagaagc ttacggccat tcatatgatc gggaacttgg ctttttagcg gtacatggat
    65341 tcttgcatat caacgggtat gaccatatga caccagaaga tgagaaagaa atgtttggtt
    65401 tacagaaaga gatcttggat gcctatggac tcaaaagata aaaacgtaga gaaaaacaaa
    65461 catttcctga tgtctgtgga atttgctgtc caaggagtca aaaccgtttt tgaggaagaa
    65521 cgcaatatga ggaaacatgt tgcgttcggt atccttgctt tatttatggg cgtgattttc
    65581 cagcttgatc ggatagagtg gttatggctg cttttagctg tttttctcgt ttggatagta
    65641 gagatcatca atactgtttt tgaaaatgta gtggacatgt ttacggattt tcattttcat
    65701 ccgattggga aaaaaatcaa agatatggca gctggagcag ttttagtgac cgcttgtttt
    65761 gctgtcatca ttgggttgat tttatttgtc cctaaaatat atcatttgtt gtttaattaa
    65821 aaaggagaac gtatatgcca gaacataaat cagggtttgt cgcaatcgtc ggccgcccga
    65881 atgtaggaaa atccacatta ttgaaccgaa tcgtgggtca aaaaattgcc attatgagtg
    65941 acaaggcaca gacaactaga aataaaatcc aaggagtata tactacacca gaagctcagc
    66001 tgatttttat cgatacacca ggaatccata aaccaaagca tcgtctagga gattttatgg
    66061 tagaagcagc ttacagtgcg ttgaaagaag tggatgcagt cttatttatg atcagtgctg
    66121 atcaaaaacg tggccgggga gacgatttta ttattgaacg tctaaaaaac gtgcattctc
    66181 ctgtttatct gattatcaat aaaatcgata agatccatcc agacgagttg ctgggaatca
    66241 tcgaagatta ttcgacgcag atgccttttg cacaagtgat tccgatctct gctacagaag
    66301 gaaacaatgt cgaacgctta atggatgtat tagtcagtga aatgccagag ggcccacaat
    66361 attttccaga tgatcaagtg actgatcatc ctgaatactt catcgtttct gaattgatcc
    66421 gtgagaaagt cttgttttta acacgagacg aagtgcctca ttctgtcgca gtcgttgttg
    66481 attcgatgaa gcgaaacgag aatgataaaa tccatatcca ggccacgatc atcgtcgaaa
    66541 gagatagcca aaaaggaatc attatcggca aaggcggaaa aatgctgaag caagtaggaa
    66601 cgaaagcaag aaaggatatc gaaaatcttt taggagacaa agtatttcta gaactttggg
    66661 tcaaagtcca aaaagactgg cgagacaaaa aagtctatct gcaagacttc ggctaccgaa
    66721 aagacgagta ctgatattct ggacttctta taggttaggt atagaaataa actggataaa
    66781 aatccgtacg ttgagaaaaa cagcggaaga tcgaaacata aataatctgg atcggcagaa
    66841 tctgcttttc cagatttatt ttttaggagg gagaatatga gccagcttgc agaatcgaaa
    66901 ggcttgattt tatctagccg taattacaaa gaaaaagata aattagtcaa aatctttacg
    66961 gaatcttctg gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa tcagccatta
    67021 gctccggctt tgctcccctt taccgaagca gtctatatcg gtaattttcg aagtgaaggt
    67081 ttgtcgtttt taagcagtgt caaagacgtc catgcgtttc ggaacatcca agaagatatc
    67141 tttttgagcg gttatggtac gtatttgcta aatctagtag atgcagcaat cgaagatcat
    67201 caatatgacc cgcatttatt ccaatttacg caacaagctc tgcagcggat ggaccaagga
    67261 gatgatgcag aaatcatcac gaatatcttt gaagtgcaaa tactgcaacg cttcggaatc
    67321 gcgccaattt ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt tgactattct
    67381 tccaaatatg gcggtgtgat atgcgagaaa cactggccaa tggacgaaca ccgttatcat
    67441 gcagatcctc gcgctgttta tttcgttcga atgttttcgg ctatttctta tgataagatc
    67501 agtggaatcc acttgaaaga agaaacgaag caggcaatcc gtgacctcat tgaccagttg
    67561 tatgatgaat atgtcggtat tcatctaaaa agcaaaaagt tcattgatca aatgaagtct
    67621 tgggcacacg tattaaagac gccagagaaa aatatgcctg aaaaagagat acctgaaaaa
    67681 gaagaataaa tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata cagggacttc
    67741 cgtctattat taaccgtaga ttaaatagat cgttcactga gaggatctat tatacaaacc
    67801 agaaaactgc aaaatgaata cgtacattga ccaagatcag taaaaaacac gtggtttagc
    67861 gagttcggga gagtggaagc cggacccaaa cgatttttga agaggcactt gcgagtacga
    67921 taaatgaagc tgccgaatct ttattcggaa gtagggtgga accgcgaaat tattcgtccc
    67981 tatgtctgta atcagacata gggacttttt gtcgttttct ttcctgtatt cttattgaca
    68041 gcaacgctta aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc gtccaagata
    68101 tgattttaac attacaaaaa ttttggtcat caaacggctg catgctgatg caagcttatg
    68161 atacagaaaa aggagcaggt acgatgagcc catatacatt cttacgggca atcggaccgg
    68221 aaccatggaa tgctgcttat gtagagccat cccgccgacc agctgacggg cgttatggag
    68281 aaaacccaaa ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa ccttcacctg
    68341 aaaatatcca agagctttat ttagaaagct tgcgtctttt aggaattgat ccattagaac
    68401 acgatattcg ctttgttgaa gacaactggg agaacccatc gatgggttgt gccggtgtgg
    68461 gctgggaagt atggcttgat gggatggaga tcacacaatt cacatacttc cagcaagtcg
    68521 gaggactacc ttgtaaacca gttacttctg agatcactta tggactagaa cgtcttgctt
    68581 cttatatcca agaagttgaa agtgtttatg atctggaatg ggctgatggc gtgaaatatg
    68641 gtgagatctt caaacaacca gaatatgaac attctaaata ttcttttgaa gtaagcgacc
    68701 aagacctgct actaagcaat tttgatcgtt ttgaaaaaga agctaaacgc tgtatcgatg
    68761 aaaatcttgt tcatccagcg tatgattata ttttgaaatg tagccataca ttcaatttat
    68821 tagatgctcg cggtgcagta tccgtaacag agcgtgcagg ttatctttcc cgtatccgca
    68881 acatggctcg tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta ggattccctt
    68941 tattgaataa aaacacagca aatccagaaa acgtgaagga ggctgaataa gatggcaaaa
    69001 gacttattac ttgaaatcgg gctggaagaa atacctgctc atgtagtcac accaagcatg
    69061 aaacaattag aagaaaaaac agcaaaattt ttagatgaaa accaattgac ttatgatacg
    69121 atcgaaacgt tttctacacc gcgccgctta gcaatcaaag taacaaacat tccagaacgt
    69181 caagcagaca gcgaagaaga agtcaaagga ccagccaaaa aaattgcttt ggacgcagat
    69241 ggaaactgga cgaaagcagc tgaaggattt gtccgcggac aaggtctgac gacagatgct
    69301 atcactttta gagaattaaa aggcgtggaa tatgtctatg taacgaaata cactcacggc
    69361 aaagaagcaa aagaagtatt gacaggttta gcagatgtca ttaaaagcct cactttccca
    69421 gtaacgatgc actgggcaga atacgatttt gaatatattc gccctattca ctggatcgtg
    69481 gcattgttag atgacgaaat aatcccgttt gagatcttgg atgtcaaaac agacagagct
    69541 tcaagaggtc atcgtttctt aggcgaagat gtgacattta cgcatgcaaa acaatacgaa
    69601 gaaaaattga atgaacaatt tgttattgct tcaccgcaaa aaagaaaaga aatgatcatc
    69661 acacaagcaa acgaattagc agaaaaaaat cactggatca ttgatctaga tgaagattta
    69721 ctagaagaag tcaacaattt agtcgaatat ccaactgtct tttctggcga cttcgaagaa
    69781 aaatatctat ctgttcctga agaagtacta gtcacttcta tgaaagaaca tcaacgttat
    69841 ttcgaagtac ggaacgatca aggtatgttg cttcctcatt ttatttctgc tcgtaatggc
    69901 gatcgggtaa aattggaaaa tgtggtacga ggcaatcaaa aagtattgac tgctcgttta
    69961 gaagatgcgg aattctttta taatgaagac aagaaagctt caatcgacca atacgttgaa
    70021 aaattaaaaa ctgttacttt ccatgaaaaa atcggtagca tctatgagaa aatgcaacgg
    70081 gtacaagtca tcgctcgcat cataggcgaa caagtaggac tatctgagga agaattgatg
    70141 gatctgcaac gcgctgctga aatctataaa tttgatctag taaccaacat ggtaggagaa
    70201 ttccctgaac tacaagggat catgggcgaa aaatatgctc tgcttaaagg cgagaaacca
    70261 gccgtagcaa cagcgatccg tgaacattac atgcctatat ccagtgaagg agatttacct
    70321 gagactacag taggttctgt tttagcgatt gctgataaat tagatagtat cttttctttc
    70381 tttgcagttg ggatgatccc aagtggctca aacgatccat atgctttgcg ccgtcaagca
    70441 tatggtgtcg ttcgaatcat cgaatcgaaa aaatgggcct ttcctttgtc tgttctgcaa
    70501 gaaacgatta gtgaagttat cagtaaagat acggatcgtt ttggcattgg cttgtcagcg
    70561 ggacaacaac aggtgatcga ctttatcaaa ggccgtctgc gccaattatt gacaacgaag
    70621 aatatccgtc atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga tctgacaaaa
    70681 gtatttgcag cagcacaatt gttcaagcag catcttgcag atgaagactt caaaccatcg
    70741 atggaagctt tgacacgtgt cgtgaactta gcgaaaaaag cggagcttga aaaacaatca
    70801 gaagtagacc ctgaactgtt cgaaaacgaa gcggagaaag aactgcacaa agcagtagag
    70861 gcaatgaaaa caagctttgc tgaccaaacg atcaacgaaa attatgatca gcttgtaagc
    70921 cttcgcccac tgatcgaaaa ttattttgat cagacgatgg tcatggcaga agaaccagca
    70981 gtacgtaaga accgattaac gcaattaagt caaattgctg ccatggcttt gtcactagca
    71041 agtttagatc aaatcatcac aaaataattt ctaatatgta aataaaccag agaacaggca
    71101 caaaataggc tatttctctg gtttatttgt tataaatata aataactatt attaattcaa
    71161 tgttaatttt tttgttattt tattttttgt gtaactttta aa
//
LOCUS       DANJMA010000009        65551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000009 DANJMA010000000
VERSION     DANJMA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            202..720
                     /locus_tag="PWU31_000712"
     CDS             202..720
                     /locus_tag="PWU31_000712"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1083280.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            848..1531
                     /locus_tag="PWU31_000713"
     CDS             848..1531
                     /locus_tag="PWU31_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL1083281.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1840..1913
                     /locus_tag="PWU31_000714"
     tRNA            1840..1913
                     /locus_tag="PWU31_000714"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1874..1876,aa:Arg,seq:tct)
     gene            2072..2431
                     /locus_tag="PWU31_000715"
     CDS             2072..2431
                     /locus_tag="PWU31_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL1083282.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2554..4185
                     /locus_tag="PWU31_000716"
     CDS             2554..4185
                     /locus_tag="PWU31_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL1083283.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4247..4888)
                     /locus_tag="PWU31_000717"
     CDS             complement(4247..4888)
                     /locus_tag="PWU31_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1083284.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5074..5150
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5170..5478
                     /gene="rplU"
                     /locus_tag="PWU31_000718"
     CDS             5170..5478
                     /gene="rplU"
                     /locus_tag="PWU31_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL1083285.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5505..5849
                     /locus_tag="PWU31_000719"
     CDS             5505..5849
                     /locus_tag="PWU31_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL1083286.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5862..6155
                     /gene="rpmA"
                     /locus_tag="PWU31_000720"
     CDS             5862..6155
                     /gene="rpmA"
                     /locus_tag="PWU31_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL1083287.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6589..9396
                     /locus_tag="PWU31_000721"
     CDS             6589..9396
                     /locus_tag="PWU31_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1083288.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9587..10078
                     /locus_tag="PWU31_000722"
     CDS             9587..10078
                     /locus_tag="PWU31_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1083289.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10181..11170
                     /gene="manX"
                     /locus_tag="PWU31_000723"
     CDS             10181..11170
                     /gene="manX"
                     /locus_tag="PWU31_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL1083290.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11203..12006
                     /locus_tag="PWU31_000724"
     CDS             11203..12006
                     /locus_tag="PWU31_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL1083291.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12027..12941
                     /locus_tag="PWU31_000725"
     CDS             12027..12941
                     /locus_tag="PWU31_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL1083292.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13024..13398
                     /locus_tag="PWU31_000726"
     CDS             13024..13398
                     /locus_tag="PWU31_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL1083293.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13537..13782
                     /locus_tag="PWU31_000727"
     CDS             13537..13782
                     /locus_tag="PWU31_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1083294.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13954..15018
                     /locus_tag="PWU31_000728"
     CDS             13954..15018
                     /locus_tag="PWU31_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL1083295.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15264..15689
                     /locus_tag="PWU31_000729"
     CDS             15264..15689
                     /locus_tag="PWU31_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1083296.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15682..16131
                     /gene="nusB"
                     /locus_tag="PWU31_000730"
     CDS             15682..16131
                     /gene="nusB"
                     /locus_tag="PWU31_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL1083297.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16228..17082
                     /locus_tag="PWU31_000731"
     CDS             16228..17082
                     /locus_tag="PWU31_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL1083298.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17083..18420
                     /locus_tag="PWU31_000732"
     CDS             17083..18420
                     /locus_tag="PWU31_000732"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL1083299.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18425..18649
                     /locus_tag="PWU31_000733"
     CDS             18425..18649
                     /locus_tag="PWU31_000733"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL1083300.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18651..19541
                     /locus_tag="PWU31_000734"
     CDS             18651..19541
                     /locus_tag="PWU31_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL1083301.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19550..20368
                     /locus_tag="PWU31_000735"
     CDS             19550..20368
                     /locus_tag="PWU31_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL1083302.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20475..20924
                     /locus_tag="PWU31_000736"
     CDS             20475..20924
                     /locus_tag="PWU31_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL1083303.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20942..22624
                     /gene="recN"
                     /locus_tag="PWU31_000737"
     CDS             20942..22624
                     /gene="recN"
                     /locus_tag="PWU31_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL1083304.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22679..22795)
                     /locus_tag="PWU31_000738"
     CDS             complement(22679..22795)
                     /locus_tag="PWU31_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL1083305.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22868..23824)
                     /locus_tag="PWU31_000739"
     CDS             complement(22868..23824)
                     /locus_tag="PWU31_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1083306.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23995..24363
                     /locus_tag="PWU31_000740"
     CDS             23995..24363
                     /locus_tag="PWU31_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL1083307.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24533..24964
                     /gene="mraZ"
                     /locus_tag="PWU31_000741"
     CDS             24533..24964
                     /gene="mraZ"
                     /locus_tag="PWU31_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL1083308.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24985..25944
                     /gene="rsmH"
                     /locus_tag="PWU31_000742"
     CDS             24985..25944
                     /gene="rsmH"
                     /locus_tag="PWU31_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL1083309.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLRQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25949..26350
                     /gene="ftsL"
                     /locus_tag="PWU31_000743"
     CDS             25949..26350
                     /gene="ftsL"
                     /locus_tag="PWU31_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL1083310.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26351..28543
                     /locus_tag="PWU31_000744"
     CDS             26351..28543
                     /locus_tag="PWU31_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL1083311.1"
                     /translation="MSLKNKIWRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVTDYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28569..29531
                     /locus_tag="PWU31_000745"
     CDS             28569..29531
                     /locus_tag="PWU31_000745"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL1083312.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29545..30906
                     /locus_tag="PWU31_000746"
     CDS             29545..30906
                     /locus_tag="PWU31_000746"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL1083313.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30932..32020
                     /gene="murG"
                     /locus_tag="PWU31_000747"
     CDS             30932..32020
                     /gene="murG"
                     /locus_tag="PWU31_000747"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL1083314.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32035..33255
                     /locus_tag="PWU31_000748"
     CDS             32035..33255
                     /locus_tag="PWU31_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL1083315.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33411..34736
                     /gene="ftsA"
                     /locus_tag="PWU31_000749"
     CDS             33411..34736
                     /gene="ftsA"
                     /locus_tag="PWU31_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL1083316.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34759..36000
                     /gene="ftsZ"
                     /locus_tag="PWU31_000750"
     CDS             34759..36000
                     /gene="ftsZ"
                     /locus_tag="PWU31_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL1083317.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36017..36694
                     /locus_tag="PWU31_000751"
     CDS             36017..36694
                     /locus_tag="PWU31_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL1083318.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36708..37307
                     /locus_tag="PWU31_000752"
     CDS             36708..37307
                     /locus_tag="PWU31_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL1083319.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37322..37609
                     /locus_tag="PWU31_000753"
     CDS             37322..37609
                     /locus_tag="PWU31_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL1083320.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37658..38440
                     /locus_tag="PWU31_000754"
     CDS             37658..38440
                     /locus_tag="PWU31_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL1083321.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38461..39168
                     /locus_tag="PWU31_000755"
     CDS             38461..39168
                     /locus_tag="PWU31_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL1083322.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39430..42228
                     /gene="ileS"
                     /locus_tag="PWU31_000756"
     CDS             39430..42228
                     /gene="ileS"
                     /locus_tag="PWU31_000756"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL1083323.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42277..43803)
                     /locus_tag="PWU31_000757"
     CDS             complement(42277..43803)
                     /locus_tag="PWU31_000757"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL1083324.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43818..44465)
                     /locus_tag="PWU31_000758"
     CDS             complement(43818..44465)
                     /locus_tag="PWU31_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL1083325.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44649..44978)
                     /locus_tag="PWU31_000759"
     CDS             complement(44649..44978)
                     /locus_tag="PWU31_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL1083326.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45155..45883
                     /gene="sfsA"
                     /locus_tag="PWU31_000760"
     CDS             45155..45883
                     /gene="sfsA"
                     /locus_tag="PWU31_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL1083327.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45899..46912
                     /locus_tag="PWU31_000761"
     CDS             45899..46912
                     /locus_tag="PWU31_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1083328.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFYVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46912..48189
                     /locus_tag="PWU31_000762"
     CDS             46912..48189
                     /locus_tag="PWU31_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1083329.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKKKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48252..50954
                     /locus_tag="PWU31_000763"
     CDS             48252..50954
                     /locus_tag="PWU31_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1083330.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKKEFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51106..51423
                     /locus_tag="PWU31_000764"
     CDS             51106..51423
                     /locus_tag="PWU31_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1083331.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51453..51773
                     /locus_tag="PWU31_000765"
     CDS             51453..51773
                     /locus_tag="PWU31_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1083332.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51881..53341
                     /locus_tag="PWU31_000766"
     CDS             51881..53341
                     /locus_tag="PWU31_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1083333.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53409..53630
                     /locus_tag="PWU31_000767"
     CDS             53409..53630
                     /locus_tag="PWU31_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083334.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53661..53843
                     /locus_tag="PWU31_000768"
     CDS             53661..53843
                     /locus_tag="PWU31_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL1083335.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53843..54256
                     /locus_tag="PWU31_000769"
     CDS             53843..54256
                     /locus_tag="PWU31_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL1083336.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54379..55560
                     /locus_tag="PWU31_000770"
     CDS             54379..55560
                     /locus_tag="PWU31_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083337.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55631..55795)
                     /locus_tag="PWU31_000771"
     CDS             complement(55631..55795)
                     /locus_tag="PWU31_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083338.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55949..56022
                     /locus_tag="PWU31_000772"
     tRNA            55949..56022
                     /locus_tag="PWU31_000772"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55983..55985,aa:Arg,seq:cct)
     gene            complement(56091..57230)
                     /locus_tag="PWU31_000773"
     CDS             complement(56091..57230)
                     /locus_tag="PWU31_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1083339.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57529..58164)
                     /locus_tag="PWU31_000774"
     CDS             complement(57529..58164)
                     /locus_tag="PWU31_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083340.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58277..58912)
                     /locus_tag="PWU31_000775"
     CDS             complement(58277..58912)
                     /locus_tag="PWU31_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL1083341.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58946..59407)
                     /locus_tag="PWU31_000776"
     CDS             complement(58946..59407)
                     /locus_tag="PWU31_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL1083342.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59537..59968)
                     /locus_tag="PWU31_000777"
     CDS             complement(59537..59968)
                     /locus_tag="PWU31_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL1083343.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59986..60306)
                     /locus_tag="PWU31_000778"
     CDS             complement(59986..60306)
                     /locus_tag="PWU31_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1083344.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60605..61381
                     /locus_tag="PWU31_000779"
     CDS             60605..61381
                     /locus_tag="PWU31_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL1083345.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61396..61599
                     /locus_tag="PWU31_000780"
     CDS             61396..61599
                     /locus_tag="PWU31_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083346.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61615..61935
                     /locus_tag="PWU31_000781"
     CDS             61615..61935
                     /locus_tag="PWU31_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL1083347.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            61940..62110
                     /locus_tag="PWU31_000782"
     CDS             61940..62110
                     /locus_tag="PWU31_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083348.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(62117..62899)
                     /locus_tag="PWU31_000783"
     CDS             complement(62117..62899)
                     /locus_tag="PWU31_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083349.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            62950..63657
                     /locus_tag="PWU31_000784"
                     /pseudo
     CDS             62950..63657
                     /locus_tag="PWU31_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
     gene            63835..64176
                     /locus_tag="PWU31_000785"
     CDS             63835..64176
                     /locus_tag="PWU31_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083350.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            64169..64840
                     /locus_tag="PWU31_000786"
     CDS             64169..64840
                     /locus_tag="PWU31_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL1083351.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64846..65532
                     /locus_tag="PWU31_000787"
     CDS             64846..65532
                     /locus_tag="PWU31_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083352.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT        21749 a        10983 c        13699 g        19120 t
ORIGIN      
        1 ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa
       61 taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac
      121 atttttacag ctataatttg aaaaaaattt atcgttatta tatgattaag aaggattgaa
      181 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
      241 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
      301 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
      361 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
      421 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
      481 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
      541 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
      601 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
      661 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
      721 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
      781 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
      841 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
      901 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
      961 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
     1021 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
     1081 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
     1141 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
     1201 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
     1261 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
     1321 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
     1381 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
     1441 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
     1501 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
     1561 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
     1621 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
     1681 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
     1741 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
     1801 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
     1861 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
     1921 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
     1981 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
     2041 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
     2101 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
     2161 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
     2221 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
     2281 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
     2341 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
     2401 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
     2461 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
     2521 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
     2581 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
     2641 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
     2701 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
     2761 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
     2821 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
     2881 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
     2941 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
     3001 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
     3061 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
     3121 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
     3181 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
     3241 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
     3301 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
     3361 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
     3421 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
     3481 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
     3541 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
     3601 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
     3661 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
     3721 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
     3781 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
     3841 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
     3901 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
     3961 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
     4021 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
     4081 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
     4141 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
     4201 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
     4261 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
     4321 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
     4381 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
     4441 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
     4501 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
     4561 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
     4621 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
     4681 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
     4741 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
     4801 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
     4861 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
     4921 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
     4981 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
     5041 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
     5101 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
     5161 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
     5221 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
     5281 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
     5341 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
     5401 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
     5461 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
     5521 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
     5581 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
     5641 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
     5701 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
     5761 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
     5821 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
     5881 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
     5941 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
     6001 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
     6061 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
     6121 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
     6181 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
     6241 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
     6301 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
     6361 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
     6421 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
     6481 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
     6541 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
     6601 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
     6661 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
     6721 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
     6781 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
     6841 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
     6901 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
     6961 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
     7021 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
     7081 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
     7141 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
     7201 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
     7261 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
     7321 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
     7381 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
     7441 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
     7501 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
     7561 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
     7621 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
     7681 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
     7741 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
     7801 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
     7861 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
     7921 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
     7981 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
     8041 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
     8101 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
     8161 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
     8221 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
     8281 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
     8341 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
     8401 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
     8461 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
     8521 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
     8581 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
     8641 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
     8701 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
     8761 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
     8821 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
     8881 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
     8941 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
     9001 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
     9061 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
     9121 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
     9181 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
     9241 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
     9301 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
     9361 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
     9421 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
     9481 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
     9541 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
     9601 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
     9661 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
     9721 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
     9781 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
     9841 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
     9901 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
     9961 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
    10021 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
    10081 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
    10141 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    10201 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    10261 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    10321 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    10381 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    10441 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    10501 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    10561 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    10621 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    10681 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    10741 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    10801 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    10861 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    10921 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    10981 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    11041 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    11101 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    11161 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    11221 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    11281 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    11341 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    11401 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    11461 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    11521 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    11581 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    11641 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    11701 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    11761 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    11821 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    11881 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    11941 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    12001 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    12061 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    12121 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    12181 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    12241 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    12301 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    12361 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    12421 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    12481 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    12541 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    12601 tcgtattagc tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa
    12661 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    12721 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    12781 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    12841 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    12901 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    12961 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    13021 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    13081 ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg
    13141 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    13201 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    13261 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    13321 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    13381 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    13441 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    13501 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    13561 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    13621 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    13681 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    13741 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    13801 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    13861 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    13921 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    13981 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    14041 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    14101 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    14161 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    14221 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    14281 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    14341 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    14401 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    14461 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    14521 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    14581 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    14641 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    14701 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    14761 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    14821 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    14881 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    14941 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    15001 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    15061 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    15121 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    15181 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    15241 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    15301 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    15361 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    15421 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    15481 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    15541 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    15601 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    15661 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    15721 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    15781 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    15841 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    15901 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    15961 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    16021 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    16081 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    16141 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    16201 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    16261 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    16321 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    16381 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    16441 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    16501 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    16561 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    16621 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    16681 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    16741 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    16801 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    16861 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    16921 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    16981 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    17041 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    17101 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    17161 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    17221 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    17281 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    17341 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    17401 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    17461 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    17521 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    17581 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    17641 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    17701 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    17761 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    17821 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    17881 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    17941 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    18001 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    18061 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    18121 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    18181 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    18241 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    18301 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    18361 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    18421 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    18481 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    18541 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    18601 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    18661 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatattgtct tttttagatg
    18721 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    18781 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    18841 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    18901 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    18961 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    19021 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    19081 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    19141 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc
    19201 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    19261 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    19321 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    19381 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    19441 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    19501 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    19561 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    19621 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    19681 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    19741 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    19801 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    19861 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    19921 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    19981 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    20041 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    20101 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    20161 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    20221 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    20281 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    20341 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    20401 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    20461 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    20521 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    20581 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    20641 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    20701 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    20761 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    20821 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    20881 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    20941 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    21001 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    21061 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    21121 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    21181 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    21241 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    21301 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    21361 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    21421 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    21481 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    21541 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    21601 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    21661 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    21721 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    21781 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    21841 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    21901 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    21961 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    22021 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    22081 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    22141 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    22201 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    22261 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    22321 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    22381 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    22441 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    22501 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    22561 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    22621 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    22681 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    22741 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    22801 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    22861 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    22921 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    22981 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    23041 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    23101 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    23161 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    23221 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    23281 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    23341 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    23401 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    23461 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    23521 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    23581 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    23641 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    23701 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    23761 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    23821 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    23881 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    23941 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    24001 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    24061 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    24121 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    24181 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    24241 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    24301 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    24361 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    24421 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    24481 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    24541 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    24601 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    24661 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    24721 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    24781 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    24841 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    24901 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    24961 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    25021 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    25081 ggcggtgccg gacatagtga atatttatta agacaactaa gttctaaagg gcatctgtat
    25141 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    25201 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    25261 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    25321 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    25381 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    25441 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    25501 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    25561 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    25621 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    25681 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    25741 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    25801 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    25861 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    25921 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    25981 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    26041 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    26101 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    26161 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    26221 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    26281 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    26341 agtgaagtaa atgagtctta aaaataaaat ttggcggttt atggaaaaga aaaatctaaa
    26401 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt
    26461 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    26521 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    26581 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    26641 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    26701 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    26761 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    26821 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    26881 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    26941 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    27001 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    27061 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    27121 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    27181 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    27241 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    27301 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    27361 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    27421 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    27481 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    27541 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    27601 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    27661 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    27721 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    27781 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    27841 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    27901 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaac
    27961 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    28021 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    28081 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    28141 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    28201 agtatccgta accgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    28261 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    28321 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    28381 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    28441 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    28501 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    28561 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    28621 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    28681 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    28741 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    28801 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    28861 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    28921 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    28981 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    29041 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    29101 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    29161 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    29221 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    29281 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    29341 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    29401 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    29461 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    29521 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    29581 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    29641 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    29701 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    29761 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    29821 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    29881 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    29941 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    30001 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    30061 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    30121 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    30181 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    30241 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    30301 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    30361 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    30421 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    30481 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    30541 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    30601 gttttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    30661 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    30721 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    30781 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    30841 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    30901 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    30961 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    31021 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    31081 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    31141 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    31201 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    31261 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    31321 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    31381 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    31441 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    31501 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    31561 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    31621 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    31681 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    31741 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    31801 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    31861 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    31921 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    31981 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    32041 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    32101 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    32161 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    32221 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    32281 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    32341 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    32401 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    32461 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    32521 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    32581 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    32641 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    32701 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    32761 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    32821 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    32881 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    32941 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    33001 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    33061 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    33121 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    33181 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    33241 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    33301 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    33361 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    33421 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    33481 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    33541 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    33601 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    33661 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    33721 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    33781 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    33841 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    33901 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    33961 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    34021 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    34081 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    34141 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    34201 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    34261 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    34321 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    34381 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    34441 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    34501 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    34561 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    34621 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    34681 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    34741 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    34801 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    34861 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    34921 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    34981 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    35041 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    35101 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    35161 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    35221 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    35281 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    35341 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    35401 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    35461 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    35521 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    35581 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    35641 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    35701 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    35761 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    35821 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    35881 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    35941 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    36001 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    36061 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    36121 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    36181 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    36241 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    36301 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    36361 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    36421 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    36481 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    36541 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    36601 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    36661 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    36721 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    36781 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    36841 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    36901 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    36961 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    37021 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    37081 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    37141 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    37201 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    37261 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    37321 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    37381 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    37441 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    37501 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    37561 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    37621 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    37681 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    37741 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    37801 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    37861 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    37921 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    37981 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    38041 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    38101 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    38161 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    38221 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    38281 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    38341 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    38401 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    38461 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    38521 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    38581 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    38641 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    38701 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    38761 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    38821 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    38881 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    38941 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    39001 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    39061 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    39121 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    39181 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    39241 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    39301 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    39361 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    39421 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    39481 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    39541 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    39601 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    39661 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    39721 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    39781 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    39841 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    39901 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag ctatatttac
    39961 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    40021 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    40081 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    40141 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    40201 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    40261 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    40321 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    40381 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    40441 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    40501 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    40561 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    40621 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    40681 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    40741 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    40801 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    40861 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    40921 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    40981 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    41041 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    41101 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    41161 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    41221 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    41281 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    41341 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    41401 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    41461 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    41521 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    41581 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    41641 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    41701 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    41761 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    41821 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    41881 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    41941 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    42001 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    42061 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    42121 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    42181 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    42241 ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc
    42301 acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa
    42361 agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc
    42421 ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga
    42481 gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct
    42541 attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc
    42601 ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat
    42661 cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa
    42721 tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg
    42781 tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt
    42841 gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc
    42901 cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa
    42961 cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat
    43021 ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat
    43081 atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc
    43141 tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg
    43201 agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc
    43261 taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt
    43321 caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa
    43381 gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg
    43441 aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg
    43501 gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata
    43561 gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt
    43621 ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac
    43681 agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt
    43741 acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga
    43801 catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg
    43861 acggggattt tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca
    43921 acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct
    43981 ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt
    44041 tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa
    44101 tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt
    44161 tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa
    44221 aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca
    44281 gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa
    44341 atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg
    44401 actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt
    44461 gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa
    44521 cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa
    44581 aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg
    44641 ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga
    44701 gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga
    44761 tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa
    44821 tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga
    44881 tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt
    44941 gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac caaatcaccc
    45001 ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata
    45061 aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg
    45121 ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc
    45181 tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat
    45241 aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga
    45301 agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt
    45361 aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga
    45421 tggtatttta gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa
    45481 gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca
    45541 aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc
    45601 tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga
    45661 tggttatgcc gcttatgtct tgtttattgc tcaattcgaa catcttcatc tggcaacgat
    45721 ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt
    45781 tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat
    45841 tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat
    45901 gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt
    45961 aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga
    46021 atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa
    46081 tattactcat atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc
    46141 taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc
    46201 aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc
    46261 agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga
    46321 cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaggtatt
    46381 agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt
    46441 aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta
    46501 tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga
    46561 gaatttttat gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat
    46621 ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt
    46681 tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat
    46741 ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg
    46801 attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt
    46861 aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa
    46921 tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca
    46981 ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg
    47041 gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg
    47101 gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa
    47161 gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg
    47221 ggcggagcca acgtcaaacg tcagattgag ggtttgaaga aaaaaccaga aatcttgatt
    47281 ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg
    47341 aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt
    47401 gatcaaattc ttcatcaagc tccaacagag tatcagctag cctttttttc agcgacagca
    47461 gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg
    47521 acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga
    47581 aagaaagatg actatttgag acgtctcacg catatagaag attttcatgg tctgattttc
    47641 tttaatcaac tgactgaatt aggcgtgatg gaagaaaaat tgctgtatag aggtgtaccg
    47701 gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc
    47761 aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt
    47821 acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat
    47881 cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat
    47941 aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt
    48001 tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca
    48061 gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa
    48121 gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg
    48181 cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga
    48241 ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac
    48301 aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc
    48361 gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg
    48421 tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc
    48481 aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg
    48541 caaaagctgc gattctttat ccaccgcggg ggctgcatac gattattttt ggagaaactg
    48601 gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc
    48661 tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac
    48721 tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata
    48781 gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt
    48841 taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc
    48901 ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat
    48961 cttcagaaag tttcttatct acgtttaatc gacggattcc tatggcaatc accttgccga
    49021 atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag
    49081 aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc
    49141 tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa
    49201 aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac
    49261 tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc
    49321 gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct
    49381 ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt
    49441 ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg
    49501 atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt
    49561 tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag
    49621 accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg
    49681 atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt
    49741 ttaagaagga atttcaagta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag
    49801 ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag
    49861 aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga
    49921 ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt
    49981 ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca
    50041 aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa
    50101 acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga
    50161 caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag
    50221 atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg
    50281 aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag
    50341 gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac
    50401 tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg
    50461 aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat
    50521 ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg
    50581 atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct
    50641 tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt
    50701 tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt
    50761 tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa
    50821 aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat
    50881 atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata
    50941 aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact
    51001 ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct
    51061 tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca
    51121 atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa
    51181 gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat
    51241 aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg
    51301 aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg
    51361 caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa
    51421 taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca
    51481 tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg
    51541 ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac
    51601 ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata
    51661 tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct attgcgttta
    51721 ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac
    51781 ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag
    51841 aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg
    51901 gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc
    51961 tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat
    52021 gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt
    52081 agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt
    52141 ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct
    52201 tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat
    52261 tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa
    52321 tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc
    52381 agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt
    52441 tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac
    52501 gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc
    52561 agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga
    52621 tgggcaactg atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc
    52681 tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg
    52741 tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact
    52801 aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt
    52861 agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc
    52921 atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa
    52981 acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa
    53041 cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt
    53101 gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc
    53161 tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac
    53221 aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat
    53281 ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata
    53341 agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag
    53401 gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag
    53461 aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt
    53521 tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca
    53581 aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt
    53641 aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt
    53701 tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca
    53761 caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag
    53821 aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt
    53881 tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc
    53941 agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg
    54001 ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac
    54061 aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga
    54121 ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct
    54181 tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat
    54241 cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa
    54301 gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt
    54361 gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag
    54421 gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa
    54481 cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg
    54541 ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta
    54601 atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat
    54661 cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg
    54721 gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc
    54781 agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag
    54841 cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa
    54901 aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa
    54961 ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt
    55021 tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct
    55081 ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat
    55141 tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta
    55201 atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat
    55261 ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa
    55321 tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta
    55381 gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta
    55441 gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat
    55501 gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga
    55561 taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg
    55621 aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg
    55681 ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt
    55741 ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa
    55801 aggacctccc tacattattt cattctacct ctagtttatc atctgttaag gactagtgga
    55861 aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca
    55921 aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc
    55981 ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtataaata acccctgtat
    56041 cctttgcggt acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca
    56101 tttttatata aactatcgaa cacagtattg attttttctt tatcctgatc ttcaagctct
    56161 cgaacaatat gaagataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
    56221 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgtgt atgccttagt
    56281 ccatgcaaag taaattgctt ttcaatccct aattctttta attttttgcg caaatatttg
    56341 ctgacggcgt ttgatgaaac taatccattt tttaagttga aaaaaacaaa attatgaggg
    56401 tttttgattt caaaattctc gaataattct ttttgattga ttttaaactt ttttaagaga
    56461 tcaatcgtat gtttatcaat ggaaattttc ctatttgaag tttcgttttt tgtttttccc
    56521 cattcattta atttataatt ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg
    56581 atatcttccc atgttaatcc tagaagttca gcaaatcgca ttccagtggc accagcaact
    56641 aacacgagca taggagaaga gtactgcgca gaaaggtttt cttctacgag tttcattaaa
    56701 gatttaaatt gatcataatc taaatattta tcttcttttg gcttcaatga atcttttcct
    56761 ttgattactg cttttctagt tggatcaaac ggaattaatc cttcttctac ggcatctttc
    56821 aacgacgctc taatatggtt attaaactta ataacggtag attttacatg gtctttagca
    56881 tacttattta aaaagcgttg atatcccact ctatctaaat cagaaatcaa taccgctggc
    56941 atatatttct ttatgttcat taacgtatct tcgtattttc tataagtgat aggagatact
    57001 gttccttctt tataaagttg catccaatcc tcaaagtaat ctgataataa tagatttttg
    57061 cgatcccctt taaggccttt gttcaagtta tattctaatt cgttggcggc atcttttgct
    57121 tctccttttg tcttaaatcc tgattttctt atcttcgtgt attttccgtc atcttttttg
    57181 tatgaaattt cgtattgcca actatttcca cgtttgacta atcttgccat aattgaatca
    57241 gctctctttc tctgatacaa taggaactat aaagaagccc attgtttggg ttttattatt
    57301 ttttgcacat cctttttgct tgccggcgca aggatgtgct atttgtcgtt ttaatggtat
    57361 aattggatgt gtattagatc atccaatcag aggtgtttat tatgattaaa gtgattttta
    57421 aaagaataac tgaacttcca gagtttagat tatttgttga ttattggtat gtctggtttt
    57481 ttattgttgc attgctgata ggtctagatg tcatattaga aagaaaactt aagtaagagt
    57541 tgagttaata aattgaattc ctaaatgaga tattttgtag cccatatttt gtttgccgac
    57601 atcacctaat actatggagc taatcgtttt ttctaacaga ccaaaatcag ataatctatt
    57661 taagctacca tctattagct cttgtgaata gccagacatg ctgatatttc cagtgaaatt
    57721 ttcgtttcca cgactcatta agcatgctaa caattgaatc tcaatcgatg ttaattgatt
    57781 cagtacaaga agataatact cctcgttatc ccatgaagcg ttgttaattc gaagtaaaca
    57841 attcttataa agatttttat acatatctat tttattttgt gcctttgatt tttctacctc
    57901 gtcatttatc ccttcgaata ttccaatgag ttggtccttg ttctctaagt tgaaaacatc
    57961 ttctggaatt tgatcgcgaa tgttttctaa tgattcgttt aaatttttgt agaatttctc
    58021 gatgcgtttg aaacgcttct cgtttttaga cccaaaataa agtgactgca caacgccacc
    58081 aatataagga attgctccaa taccgccttc aactattaat tcaagatatt cttttttggt
    58141 tagtttttca ttgctattat ccatagttac ctcctatttt gtcatttcct ttacaagcat
    58201 tgtgactcct gatggcactc ttttgtatag gttttattat tgtgcacaat ctaactctta
    58261 gtggggcgtg gttcatttat tttttactta ctttactatt aatcatatcc cattgagcat
    58321 aggatttttc tatgttcgat cctcttgaac taatttctcc tgtaaaagtt actttatcgc
    58381 cctttgtaaa ggtatcagca ttgtttaagt ccttcgcaaa aattacatat gaaactttag
    58441 gtgttgcaga aatatcattc caacttttat catcgtactc tttgtctgct ataatggcta
    58501 ttctagtagt cattgattcg ataacagttc cttcaaaagt atatgtttta ccactaatat
    58561 atttagtaaa agctgtattt tgctcagtag ctggagtaat agaataataa ctatctaaga
    58621 aagcacccca gtcatgatta tttgtctcta aaaattcttc aaattgagtt aggcttttat
    58681 tgctgctgtt agatgtagta gtttgagtat cagatgatga gctttcctgt ttgttattgt
    58741 catttgcctc gtttgatgag gaaagcgaga aacctacggt agtaaaaata atcccgataa
    58801 taaggagaat cataggtgtt ttcttatttg tttttttgat aaatcttaca attaacatga
    58861 ttaaacctat aataaataaa acaataccag taaaaccaaa tataccgtcc attaatagat
    58921 cctctttctc ttttgatatt gaatattata aaccaagcaa ttcttttttc ttaaaatcga
    58981 attcttcttg attaataatc ccttcatcta acaactcttt atacttttta atttcatctg
    59041 cacttgaaat ttgtgtttct ttggaattat tatctaatat agattgttgc ttctctatat
    59101 atttttttaa attttccatt tcattccaat atttttttgt aaacataacg gtgttttcat
    59161 ctttcgtggc cgctaatata cctccgcgac tttcgtttcc gcctagcaag gtaaattgta
    59221 tataaccatt actcatacct ggttttttta attgtacggc tgatatattt ttaaagggga
    59281 tagttttttc tcctttaata ccttgattaa ctaaatttat aaatcctttt ctaataattg
    59341 aaatagaatc atcagttact ttaatcaagg tttttcctgg agattttatt aaaatctctt
    59401 tttccatttt ttcttcctca tttctgtgat atgatttttt ttgtagaagc tcatatatga
    59461 ggaaagagcc cgtgttgcag cacgggcttt tttaatgttt tgctgaaatc ggttttttta
    59521 ataattcttg atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc
    59581 attggcagac agcataacaa gtgtatctga gcttattctg aatagaataa tactccataa
    59641 atttttcgag attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata
    59701 aaattagaat agctatttta tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag
    59761 tatcgtacaa aactgaaaat tcaaaatgag aggcgctgaa atgtgcaagt tcatgagata
    59821 aatgaaaggc ttctgcagtt tcaccgtata gattttcatt caaaaaaatg attctgggtt
    59881 ttggatagta gaaaccaggc tctttcatct ccatataaac tactttcaaa ttgtattcgc
    59941 tcaacatttc tttcaatttc aaatacatac aaaccatcac tccaactatt cattttcctc
    60001 taaagcttta gcaattgcaa tcgctttacg cattgtctcc ttagatattt cttttccatc
    60061 aaaagaaaaa acagtatcgt cttctgataa atccacatgt ttaggggttt ctctttcttc
    60121 tctacctaga aggtagtcta cagagacatc gaaatagtca gcaatccgac ttaattcatc
    60181 tgaattagga gtattatttt tccactttgc tagataaccg ttagaatatc caaaattaat
    60241 ttctaactgc cgtatagata tctttttcct cttcgccaag tcttttatta tttcataggt
    60301 attcatcgat aaaacaacct ttctaaatgt ttacaaaaaa agtttagaaa aatacgcaga
    60361 aataatttga ctaattctga gtaatgagct atactatatc ttgtaaacag atttaacaac
    60421 taaaaagaca acaaaaaaca ctattgatat gtaaatgcag accgccaaga aagctttaaa
    60481 atcaatgttt ttatgtctta tttaattatg ctcttagtat agagtattac tcagatactg
    60541 tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga atataaaaga aaggaatgag
    60601 aaatatgaac acaccgcaaa tttttaattt cgaacaaaac gaagttcgga caattttagt
    60661 aaatgatgaa ccatattttg taggcaaaga cgttgcgagt gttttgggtt attcaaacac
    60721 taaagatgct ttgtcgcggc atgtagattt ggaagataag atggggtcgc gaatcacgac
    60781 ctcaggtcaa tcaagagaga tgacaatcat caacgaatct ggtttgtaca gtttaatctt
    60841 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacaagtg aagtgttgcc
    60901 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
    60961 tccagataca atcattcaat tagcaactca actaaaagaa gaaagaactg gaagattaat
    61021 cgcagaacaa aagattgcag agtacgaacc taaaatctcc tatttagata gcatattatc
    61081 ttctacagat tcagtaacaa ttagtcagat tgcagcagat tatgggatgt ctccacaaca
    61141 gatgaataaa ttacttcata aactaggtat tcagaaaaaa gtcggtaacc aatggttatt
    61201 gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat acaactgaga tcccgaaagc
    61261 cgatggtggc actaaaattg taatgaatac caaatggaca cagaaagggc gtctatttat
    61321 ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg gatttagagg aaattggtta
    61381 gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc
    61441 aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga
    61501 attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac
    61561 agaattatag attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa
    61621 tatctagaag caaaaatccc aattccagaa ggctatgtaa ttatctccca agtggattat
    61681 gaggagttaa aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa
    61741 cggattaaca gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga
    61801 aatattatcg atatcgagaa aaacaaaaat ggatttgtct attatccagt tgaaggaaga
    61861 gacacatatc tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa
    61921 atcttaagga ggtaagcgga tgggtaaatt taatagagca ctagtattca gcgcaccact
    61981 aattatctac gctttaggtc tttggggaag cagacaagca ttaatcggca caattgtata
    62041 tatggtctgg atctttatgg ggcttgatga agctgaatat agagcgaaaa agccagtcgg
    62101 gagggactga cttaacctaa ttaattaaaa attgtaacaa ataacgcaaa aataaagcta
    62161 atgaaattgt attgttagga tctactccaa tagttatatt tacaatttcg gttactacta
    62221 gtcctatcaa agctgcccca gtttgctcta aataccacat tttatctttg tagaaagcag
    62281 gtgaagcttg ttgtttttca gatggttcat tagttatatc tttgttcttg gctgtattta
    62341 tttcttcaaa aagctttata gaatcatcaa ggactttcct ttgctcatta aggatatcaa
    62401 aaaacttagc ctcaaactca tcagatggat tagaagtgtc tttaaaaagt agattttttg
    62461 tactaacttc cggttcggaa gaaaatgctt tttctaattc ttgggtagct acccctattc
    62521 tgaagtcgta cttagacatg tcaacggcaa atttttctgt gattttagag atatcagaaa
    62581 aataaggttt tggcatagta gctgccattt ttttagcaat attaaaactt ggagattcta
    62641 attgtttctg tattgcgaac aaagaaggag gaatcatatt agcaatagat ttgttacgtt
    62701 tctcgacaat ttgaatgaga gaatcattga gtattgcttg cgaagctata aaagtatctt
    62761 gaattgcaat taatttttta gcttgttcta tcgcaggctg catagcgtta acgacatcta
    62821 gagaaggagc aatggtttca ccgattaatt ttgaagaagc tatcaatttt tgataataag
    62881 gagtatctga aatatccaat tatatacacc acctttttac ctaaattata ccaaaaggga
    62941 gagaaaaaaa tgcaataatt agtaattttg aaaaataaag aagctgtgac tacgagctta
    63001 caagtcgcag aaacattccg aaaggaacat agagcagttt taaaggcgat tgacgatcta
    63061 aaagatgggc ttgcacaaaa gtatgcagac ctattttggg aagatgtgta catccatccg
    63121 caaaacaaac aggcttatcg aattgtctat atgaatcgtg atggctttac tcttgctgta
    63181 ttaggattca acaatactca aaaagtttta gagttcaaat tgaaatacat tgaagcgttc
    63241 aacgaaatgg aaaaggcaat tcgcaaaaat acagcttttg acacaattga agacatgatg
    63301 atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaaaatact
    63361 atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatac gaaagttatg
    63421 gaagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca
    63481 aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt
    63541 aagagattcg atgatgctgt ttcatttatt gaaatgtggt taccagaaac tgcgattcgc
    63601 atggaaatcg atcaactgaa cagacaacaa agactttttg gtgatgaaaa tgaatagagc
    63661 tgaagcgcta agaatcggga cggtaattgc taatcgctgg tggagacaca ataaaccaat
    63721 catcctaagc caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact
    63781 ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa
    63841 aaagaacttt ccactctaga tcaatatttg actgatccta gttggggcaa atcgaatatc
    63901 aaggaaacaa ataatcgaaa aatcagacga aatcttttga cggatgaaga actagcatgt
    63961 gatcaagacg atttgggaaa ttttgtgagt atttgggatc atgtctatct tatccattta
    64021 tcgaggaagt ccaaaaaacc tgaatatatc tatgtcatcg aagatggctt gattgatgcg
    64081 ctagaagagt atgacagaga taacttgatt gatatctctt attacggatc aggtaagaaa
    64141 tacattgctg aaatggaggc agaatttgat gagtgaaaac aaagggacaa cgacttttga
    64201 aaaacttttt agtcgcaagt taaataaaat actcaagaaa aaaggaaatt ttgattattt
    64261 atcttgggct cacgcatggg agattatgaa aaagaatgat ccacaggcaa cggtaactat
    64321 taatgagtat aaacactaca gagttgtttc tggaactcat caagactttc ttgttgagga
    64381 atataaacct tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa
    64441 aggacacacg gaaaccgaat tatttcctgt tttagattat cgaaaccaac cagttgttaa
    64501 accaaatgca atgcaaatca ataactcatt gaagcgatgc tttgtgaaag cattggctct
    64561 acacggactg ggattatatg tatttcaagg ggaagatatt ccaacaccac ctagaatcga
    64621 tacgaagaaa ttaaacatgc tagagacgat tctagaagct ttcaatgagc agatgggtaa
    64681 agatatgacc aaaaccttaa tcgaatatgt taatgagcag acagataaat tagggctctt
    64741 agctgataac gttgaaacta ttgaacagtt aagctatgag caatgtgcct tgatggagcg
    64801 agcaatagca gctaagagaa aagaattaga taagaagtga tatgagtgtt taaaccatta
    64861 atcgattcat attcagcggt tctgaaaaag ttcaaaggaa aagacatagg tgcaaccatc
    64921 aatgaggaag tgaacattga tcgactaaag acgatgtatg acggctacga tggtgatcga
    64981 gttattgaaa ttcgttttat tgatcctaga cgtttcacag tacagcaacg aaacttcatc
    65041 tatgcactca taggcgatat tttcatcgat acaggcatgc caacggactt ctggaaggaa
    65101 ttcttttact tccgctttga aggtgtcaca gggcgcaaaa taagcctcaa agatgaatcg
    65161 aatacgactg tgagtgatgc taacgtctta gcaaatatca tcttagattt catctttgaa
    65221 catcatattc ctttcaaaga aggctatgag attttaccag cgaatcaaga atattacttc
    65281 tacaaatgca tcacaaaaag agtctgttgc atctgtggca aaacaggagc tgatatcgat
    65341 cactttgaca aagcgctagg aagacgaaag cgcaaagaag ttgatcattc agagtacaca
    65401 tttgcagcac tctgcagaat ccatcacacg gagaaacaca agataggtgt gatcaatttc
    65461 aaaaataaat atcaaatcaa agggatcaag ttaaaccagg aaacaattaa gaaacttaga
    65521 ataggaggat aaaaaatatt ggctgacaac a
//
LOCUS       DANJMA010000010        61007 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000010 DANJMA010000000
VERSION     DANJMA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(135..1901)
                     /locus_tag="PWU31_000788"
     CDS             complement(135..1901)
                     /locus_tag="PWU31_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083353.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1861..3615)
                     /locus_tag="PWU31_000789"
     CDS             complement(1861..3615)
                     /locus_tag="PWU31_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083354.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3725..4294)
                     /locus_tag="PWU31_000790"
     CDS             complement(3725..4294)
                     /locus_tag="PWU31_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL1083355.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4312..5727)
                     /locus_tag="PWU31_000791"
     CDS             complement(4312..5727)
                     /locus_tag="PWU31_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1083356.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5738..6406)
                     /locus_tag="PWU31_000792"
     CDS             complement(5738..6406)
                     /locus_tag="PWU31_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL1083357.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6536..7120)
                     /locus_tag="PWU31_000793"
     CDS             complement(6536..7120)
                     /locus_tag="PWU31_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1083358.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7451..7684)
                     /locus_tag="PWU31_000794"
     CDS             complement(7451..7684)
                     /locus_tag="PWU31_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL1083359.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7699..8649)
                     /locus_tag="PWU31_000795"
     CDS             complement(7699..8649)
                     /locus_tag="PWU31_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL1083360.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8646..9488)
                     /locus_tag="PWU31_000796"
     CDS             complement(8646..9488)
                     /locus_tag="PWU31_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1083361.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9490..10209)
                     /locus_tag="PWU31_000797"
     CDS             complement(9490..10209)
                     /locus_tag="PWU31_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1083362.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10901..11059
                     /locus_tag="PWU31_000798"
     CDS             10901..11059
                     /locus_tag="PWU31_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083363.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11131..12357
                     /locus_tag="PWU31_000799"
     CDS             11131..12357
                     /locus_tag="PWU31_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1083364.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12946..14394)
                     /locus_tag="PWU31_000800"
     CDS             complement(12946..14394)
                     /locus_tag="PWU31_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL1083365.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPDEYVLAINSMTKPNPTFKKEMKSIKNARGEDDILFII
                     DVEPSISHVIFNSNGDCFLRIGDKSVHQTHDQISKLEYDKGQRMFEDIEILDSTLDDI
                     DESLMDSYKKLKKAEAISTETILTGRGLMRNGHLTNAGMLLFGKNTFLALPNSRIRFL
                     RYDGKRNETGRRLNIVKEFDYEGAIPKLIESITRDVKTQLREFQYLNDDGKFSIIPEY
                     PEFAWFEGIVNALTHRDYSYKGDYVRINMYDDRLEIFSPGALPNIVTLQNMRRTRYAR
                     NPRIARTLSEFGWVKELNEGVNRIYDEMQLLFLNEPKYSEPNNNSVLLVLENSITSRH
                     LRERDRLDDYLGEFEVDSLSTSEKMIVSHLFNNEKISVKEASEVLKKGVGYSRKVLKE
                     MLRKKIIQWHGTSTKDPTQYYSLNRVKQSKIE"
     gene            complement(14540..16036)
                     /locus_tag="PWU31_000801"
     CDS             complement(14540..16036)
                     /locus_tag="PWU31_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDL1083366.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKSFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(16208..16453)
                     /locus_tag="PWU31_000802"
     CDS             complement(16208..16453)
                     /locus_tag="PWU31_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1083367.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRIRLEKGIGQTELIRDLQLHKIA
                     ITRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            16558..17331
                     /locus_tag="PWU31_000803"
     CDS             16558..17331
                     /locus_tag="PWU31_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083368.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSETSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAKLYTEKVEQFFKSGANKDFSKISDYSENFFNETQDEIMNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
     gene            complement(17561..17809)
                     /locus_tag="PWU31_000804"
     CDS             complement(17561..17809)
                     /locus_tag="PWU31_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1083369.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(17908..18258)
                     /locus_tag="PWU31_000805"
     CDS             complement(17908..18258)
                     /locus_tag="PWU31_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083370.1"
                     /translation="MIFENESEEIGRMTEENKVDLLETLVINKKEFEQLFLDFFVQYD
                     RIYDFNSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDADYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(18299..18793)
                     /locus_tag="PWU31_000806"
     CDS             complement(18299..18793)
                     /locus_tag="PWU31_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083371.1"
                     /translation="MFKVYFPFQWLVKGIFFVLLGLILFGSYLTIKARYFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKSETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(18786..19088)
                     /locus_tag="PWU31_000807"
     CDS             complement(18786..19088)
                     /locus_tag="PWU31_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083372.1"
                     /translation="MSDPYIKVYCSKETKKLFDSFYEKNKGTLGTKANMFRVMVSNLP
                     MLASQSNKKFSDPESIKLEQTISELESSIMKKLDGIDQKLSCSLKTKYKTEEKKDV"
     gene            complement(19111..20448)
                     /locus_tag="PWU31_000808"
     CDS             complement(19111..20448)
                     /locus_tag="PWU31_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1083373.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     EFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQLTGKKMDIRKNDIRVLQAWNDNILIDNGINPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKNHSIETYRFAKNQ
                     RIGYKLLDSEGELILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSKEVLTEIAIRHL
                     GKSLNGRADSRFNWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIENLLDV
                     FGENKNQFFGFAVEEEPLKYKKKCVNLVYKKVVYDIAESLSTDYDQFNIQDTEPALKR
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETARGIDYQKQQNQYDMDF"
     gene            complement(20433..20930)
                     /locus_tag="PWU31_000809"
     CDS             complement(20433..20930)
                     /locus_tag="PWU31_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083374.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAEKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(21079..21417)
                     /locus_tag="PWU31_000810"
     CDS             complement(21079..21417)
                     /locus_tag="PWU31_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083375.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKYVYEKTGISSVKEFE
                     ETFEINQKGTQEKWQKAVNFANSEFTLVHEFLASDQLMETKFQQFLRRKGETAYRIFY
                     REEAQEESVR"
     gene            complement(21421..21606)
                     /locus_tag="PWU31_000811"
     CDS             complement(21421..21606)
                     /locus_tag="PWU31_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083376.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     MMKNELMNELSHIQKGA"
     gene            complement(21620..24172)
                     /locus_tag="PWU31_000812"
     CDS             complement(21620..24172)
                     /locus_tag="PWU31_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083377.1"
                     /translation="MKIIKKKIDNRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLNTVVIGLMVLSLVIIGYKMVTGKGTVDLKSVGMNVVMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNSGGNDQTEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMAS
                     ERATKPFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPI
                     RLSDRKDGAKIENADRIMSSSERMREAMKNIAESNTNYASAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKN
                     SNTGLEKSVKENLQNSTSSQDRRSVDVRENLHNTTSSQSGNRTVDVRENVQSSSTGQT
                     RTVDVRLNLQDTTSSQSGNRTVDVRENVQSSAPNQPKTVEVRETRAEASQGDKTQKIN
                     IVEERKERPNEKVIVEQEVKEKKRQRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(24159..24377)
                     /locus_tag="PWU31_000813"
     CDS             complement(24159..24377)
                     /locus_tag="PWU31_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083378.1"
                     /translation="MIENHLVDKIYCLDHNADTLELIESICSNTIVLDLRAVNDDYVT
                     HIIVNKKMAQKLSESLKKWAEGGNNENN"
     gene            complement(24370..25110)
                     /locus_tag="PWU31_000814"
     CDS             complement(24370..25110)
                     /locus_tag="PWU31_000814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083379.1"
                     /translation="MREKLKKLGDKKRHTFQGTFEFNGYKKDKYKFGQEYENATFMLS
                     NLKIVQGDNLELVTDHIWMNLTKQFLKFGWLKKGDMVQFDGRIKSYSSKKGINYKIER
                     PSKVKVFRKGTEINESSALKISTRYEIVEDIKRQNREYYEARDFFFENYFSVPRYFKL
                     KEKWPEIQAVVAINENRYTSEQFKQVFKDRDSLVDLYKKIQETDKYNLQKEFYNGLVS
                     NKNRIDLNEVEGYLHKIENFDYGRGYFD"
     gene            complement(25243..26118)
                     /locus_tag="PWU31_000815"
     CDS             complement(25243..26118)
                     /locus_tag="PWU31_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HDL1083380.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYTKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKFDMILQSGQVEPLRVDLMEKWYPAGIPCDVKSDTQLSQDQNSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIQIITRLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEEIDEMKFINTTARNHWEDIAKQE
                     KASRLDMLETLKKIG"
     gene            complement(26288..26569)
                     /locus_tag="PWU31_000816"
     CDS             complement(26288..26569)
                     /locus_tag="PWU31_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083381.1"
                     /translation="MEQIGVSVMLNTDQTNQLKTHVFEVTKEAIDQARIDSGLERPFL
                     KGKEMAKFLNVSYGTFLKFKRLGLPIIVVDTIELYSKEEVKKWMLQHQI"
     gene            complement(26624..26821)
                     /locus_tag="PWU31_000817"
     CDS             complement(26624..26821)
                     /locus_tag="PWU31_000817"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1083382.1"
                     /translation="MFQINEDFSIRIRERRGKLMITKTKAAELIGISRQTLKKIECFE
                     VQSVSKNVYQKIIGWLLKSDY"
     gene            26988..27362
                     /locus_tag="PWU31_000818"
     CDS             26988..27362
                     /locus_tag="PWU31_000818"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1083383.1"
                     /translation="MTQKNRIKETREEQNLTLKELSNLSDIPYQTLRNYEIGKRKPRN
                     DEVWWKLGTILNVRGPYLKGEDSFKTNDELHIYYLKKSHTNFELSKKEEIIDIIDKLN
                     EDGLDEALRQLALLSKIDDFKR"
     gene            27452..28618
                     /locus_tag="PWU31_000819"
     CDS             27452..28618
                     /locus_tag="PWU31_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1083384.1"
                     /translation="MSIQKYKTKSGTRYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDMGYHRPSNDLFRTVYEEWLPSYKNRVQETTYQRTKDLFRLHILPKFG
                     DKSISKITPIYCQNAINEWAVKYTNIKQLKSYSSQIFEYAIFAELLQRNPMTNTKLPK
                     QEKKESENYFSLEELKEFLEILNKEESLKHILIFQLLITTGVRKGELSALRWADINFE
                     EQLLHIGKSYATIRTEDPNATRKTIRIQKNTKNVSSERILPIDTQTIEMLKLWKKEQA
                     EVLLQFGINANTKKQLIFTYVNANGDVNQPLHADYSNNIMRRLEKKYKFKHVTIHGLR
                     HTHATLLLEGGASIKEAQDRLGHKNAETTLNTYSHVTEKAQRNAVDKFIAYTGL"
     gene            complement(28717..29109)
                     /gene="rpsI"
                     /locus_tag="PWU31_000820"
     CDS             complement(28717..29109)
                     /gene="rpsI"
                     /locus_tag="PWU31_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1083385.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29125..29568)
                     /gene="rplM"
                     /locus_tag="PWU31_000821"
     CDS             complement(29125..29568)
                     /gene="rplM"
                     /locus_tag="PWU31_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL1083386.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30087..30554)
                     /locus_tag="PWU31_000822"
     CDS             complement(30087..30554)
                     /locus_tag="PWU31_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL1083387.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30674..32212)
                     /locus_tag="PWU31_000823"
     CDS             complement(30674..32212)
                     /locus_tag="PWU31_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL1083388.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32292..32978
                     /locus_tag="PWU31_000824"
     CDS             32292..32978
                     /locus_tag="PWU31_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL1083389.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33179..33706)
                     /gene="msrA"
                     /locus_tag="PWU31_000825"
     CDS             complement(33179..33706)
                     /gene="msrA"
                     /locus_tag="PWU31_000825"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1083390.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33790..34467)
                     /gene="pgmB"
                     /locus_tag="PWU31_000826"
     CDS             complement(33790..34467)
                     /gene="pgmB"
                     /locus_tag="PWU31_000826"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL1083391.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34464..35594)
                     /locus_tag="PWU31_000827"
     CDS             complement(34464..35594)
                     /locus_tag="PWU31_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1083392.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35594..37777)
                     /locus_tag="PWU31_000828"
     CDS             complement(35594..37777)
                     /locus_tag="PWU31_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL1083393.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37774..38799)
                     /locus_tag="PWU31_000829"
     CDS             complement(37774..38799)
                     /locus_tag="PWU31_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1083394.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38844..39671)
                     /locus_tag="PWU31_000830"
     CDS             complement(38844..39671)
                     /locus_tag="PWU31_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1083395.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39668..40735)
                     /locus_tag="PWU31_000831"
     CDS             complement(39668..40735)
                     /locus_tag="PWU31_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL1083396.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40761..41597)
                     /locus_tag="PWU31_000832"
     CDS             complement(40761..41597)
                     /locus_tag="PWU31_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1083397.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41594..42463)
                     /locus_tag="PWU31_000833"
     CDS             complement(41594..42463)
                     /locus_tag="PWU31_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083398.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42533..43816)
                     /locus_tag="PWU31_000834"
     CDS             complement(42533..43816)
                     /locus_tag="PWU31_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1083399.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43830..45515)
                     /locus_tag="PWU31_000835"
     CDS             complement(43830..45515)
                     /locus_tag="PWU31_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL1083400.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45790..46815
                     /locus_tag="PWU31_000836"
     CDS             45790..46815
                     /locus_tag="PWU31_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1083401.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47410..51084)
                     /gene="rpoC"
                     /locus_tag="PWU31_000837"
     CDS             complement(47410..51084)
                     /gene="rpoC"
                     /locus_tag="PWU31_000837"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL1083402.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(51142..54759)
                     /gene="rpoB"
                     /locus_tag="PWU31_000838"
     CDS             complement(51142..54759)
                     /gene="rpoB"
                     /locus_tag="PWU31_000838"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1083403.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55487..56455
                     /locus_tag="PWU31_000839"
     CDS             55487..56455
                     /locus_tag="PWU31_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1083404.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56473..57378
                     /locus_tag="PWU31_000840"
     CDS             56473..57378
                     /locus_tag="PWU31_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083405.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57375..58181
                     /locus_tag="PWU31_000841"
     CDS             57375..58181
                     /locus_tag="PWU31_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1083406.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58317..59306
                     /locus_tag="PWU31_000842"
     CDS             58317..59306
                     /locus_tag="PWU31_000842"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL1083407.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59623..60147)
                     /locus_tag="PWU31_000843"
     CDS             complement(59623..60147)
                     /locus_tag="PWU31_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL1083408.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60434..>61007
                     /gene="scm"
                     /locus_tag="PWU31_000844"
     CDS             60434..>61007
                     /gene="scm"
                     /locus_tag="PWU31_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL1083409.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADF"
BASE COUNT        18301 a        12322 c         9920 g        20464 t
ORIGIN      
        1 attaagtgaa ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt
       61 aataaaatta ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt
      121 taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt
      181 ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc attatgagta
      241 ccttgatcaa ctagttttcc ttgatccatc actacaattt tatttacctt ttcaatagac
      301 ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc
      361 tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt
      421 ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt
      481 gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct
      541 tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca
      601 atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg
      661 attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata
      721 cagccttcat cataatcata cagtctagac actaatttca agatactagt tttcccggaa
      781 ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct
      841 aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat
      901 ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt
      961 attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc
     1021 tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt
     1081 aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag
     1141 gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct
     1201 tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct
     1261 tgctgcaatt ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat
     1321 ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac
     1381 gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata
     1441 aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat cgcatgctca
     1501 ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa
     1561 taggataatg gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct
     1621 ttataagtac tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc
     1681 aaagtaacaa acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat
     1741 tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa
     1801 ctagaaatag aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt
     1861 ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa
     1921 ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt
     1981 cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat
     2041 cataataact gttttttctt ttatcagatg ttgaaaagcc ttttgtagtt catactcatt
     2101 atctggatca atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat
     2161 tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc
     2221 aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct
     2281 tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta
     2341 aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt
     2401 gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt
     2461 gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa
     2521 tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt
     2581 gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc
     2641 atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata
     2701 tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat
     2761 tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga
     2821 agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg
     2881 aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc
     2941 gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac
     3001 ttgaattccg cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata
     3061 tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa
     3121 catggcgatt agaaccaatc caattttaaa gttaatgaaa aagcctaagg caactgataa
     3181 aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt
     3241 tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa
     3301 acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg
     3361 aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa
     3421 ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta caataattag
     3481 agactgaaga aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc
     3541 agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac taaataattt
     3601 tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac
     3661 tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataaa aggtaaacta
     3721 cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata
     3781 aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg
     3841 catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa
     3901 agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg
     3961 caacttgagg aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg
     4021 ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata
     4081 acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat
     4141 aaacaaatat gattccagat aataatccaa aaatcccggg ggatatgctg tgaccaaacg
     4201 ccccaccaat actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag
     4261 ctaaaccaat aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt
     4321 tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt
     4381 ttttattcca ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg
     4441 atcatgagta gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat
     4501 cctttccata ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt
     4561 ttttgacaat gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa
     4621 cggtaattga tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc
     4681 tttactaact atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg
     4741 gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat
     4801 ctttcctttg gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac
     4861 accgtttttc cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt
     4921 tagaatattt tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat
     4981 atagggaact tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc
     5041 agtttttaac aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg
     5101 atcaattaat tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt
     5161 attttttaaa tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga
     5221 agacggttca tccaatataa ttaatttaga gtttagcgat aaactacatc caatagaaat
     5281 tttctgccgt tcaccacttg atagcgaaaa aatgttttta tctaatatat ttgaaagatc
     5341 taataattca gctaattcat tgattctttt atccatatgc tcttttgtaa atccaaagtt
     5401 ttccatcgaa aatactaact cactcgttgt atttgttgta aagaattgtc ctctaggatc
     5461 ttgacttacg tagccaatat tttttgatat actttcttgt gttaaggatt gtagcgagtt
     5521 attttcaaat aaaatttccc caaccaattt tccgtcataa agtcttggaa taagtccatt
     5581 cattaacttt aatagtgtgg atttcccact cccagaagaa ccagtaatca atgtaagact
     5641 gccttttttt attgaaaaat taaggtcaac aagcgcttgt tccttaacat ccttatatga
     5701 aaaagaaaca ttgttaaatt caaacaattc tttcacctca aatcaaaata cttaagaaaa
     5761 aaatagtaac ggaaaacagg atgatatagt ctaataagct catgtttgtt ttatagtaag
     5821 aacttctttt cacaggagat tctatcccct ttgttattgc attagcggtc atttcttcag
     5881 caatcatagt tgcacgtata agaacgggaa ctaacagaaa ttccaaatat tgcagaggct
     5941 gaaaaaaaga caagcgaatg tttctcattt ttaaagattc tttaatcatt cttatttcag
     6001 aacgataagt cggaaagaat ctgatcacta ctgtaatact taacattatt tttttcggta
     6061 aatgtatttt ttctaaagag gataataatt cattgctagt atagacacta accaacaacc
     6121 atgagatcat tacgaccggc aacattcgaa atacaatata taaggatgtc gcaaaaaata
     6181 tagtaaaaaa ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat
     6241 aaatcaagat gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag
     6301 caataatgaa acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa
     6361 aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa
     6421 aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt
     6481 gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt tttaattaac
     6541 tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt aatagcatag
     6601 tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa
     6661 tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt ccgatagtct
     6721 ttaacttatc tagacaacag actgtctttt tcgtcacaaa tgatgtaatt ttttcaacaa
     6781 tatcttgata tttggttcca gctgattgga ataaaatcaa atcgaatata cgtttattct
     6841 caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa cttttctcta
     6901 tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac atattaataa
     6961 gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa gtaaaatgtt
     7021 tataaaaagc acccgtagtt acgcctgcgg tcaggcacag ttgccttatc ttagtttgct
     7081 gaaatccttc tttttcaaaa tggcttagtc cactttctat taacttttgg tgagttataa
     7141 tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact ttattatctt
     7201 acacaggact tgaagtttca atcgttaacg tttattactg aactcattcc ttagagctaa
     7261 actaatgaga aagagcagaa taactgatac aaaaagcaat cctacaagaa ttaatagact
     7321 catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa attatatcca
     7381 gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc ctccctaaaa
     7441 ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat aaattttttc cctttatcag
     7501 ttaattgaaa tcctatatct aaatagtcct catatctaag tttagccagc atctcactta
     7561 cagatgctgg actaacactt agaacttttg ctaaatcatt attttttaat ttcttttttc
     7621 tttttgataa ttcctgaatt gctagtaaat aaattagtat agtagtcgtt ttaatttgta
     7681 ccatacctac cctccaaatt atttcgaaag gccttcagca attttatcca ggttccattt
     7741 catcatcgca tagtaactat caccatcttg tcctttttct gcaattgaat cagtaaaaat
     7801 cgttgaatag ataggaatat tggtatcttt tgatactgtt ttcattggtc tatcatccac
     7861 actgctttct acgaataagg agggaacttt tgttgtgcgt aatttttcaa ctaagtgttt
     7921 tatttgatct ggtgttcctt cttcttcagt gttgatttcc caaatgtaag cagaaggcac
     7981 attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca tttttttatc
     8041 attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca atttttcaat
     8101 atacttatct agattttctt tatagaattt tttattatca ggatcttttt ccgctaactg
     8161 tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag catgaggatc
     8221 ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat cacttgctgc
     8281 aaaatagtct ttgttttcct ctttgttcgc atttttaact aattttgtaa accaagcatt
     8341 tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg aagttttttg
     8401 aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta cgatactgtg
     8461 taaatcaatt ttatcttttg ctatattttc ggtaatatct gctaaaatcg aattagtaac
     8521 tactactttt aatttttcgt tcgaagccac tttatctttg tctttactat ttattgaaca
     8581 tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg ctttccattt
     8641 tctcatcata acgatctctc cttctttctt aaaaattttt gttttggtga gattagaaaa
     8701 ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc tatgttaaaa
     8761 ctataaccaa taaacaatcc aagcaatgat gaacttgctc cgactgttgc agaaataaat
     8821 atcatagttt tcaagctatt tgcatataaa tatgctgtag ctgctggtgt aattaacatt
     8881 gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa agttaataga
     8941 accatcaata gataatgata aaaactgaca ttcatcccta ttgcttttgc cattaatgga
     9001 tcaaaagaag tgattaataa ttctttaaaa aatagaacta ctataaccaa cactaaaacc
     9061 gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt tccaaataaa
     9121 atatgaaata aatccgttga gctatttgca actcctataa ggataactcc tagtgctaaa
     9181 aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt gctttttata
     9241 aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa aaaattaata
     9301 cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc atcacccata
     9361 agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat ccctataact
     9421 acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt aataaaattt
     9481 tgaagcatat tattccccct ctccaataaa tatagtatct ccataggctt tttttaaatt
     9541 ttcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat tcaaaagaat
     9601 aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta gcactgtttt
     9661 tccctgatgt ttcaattttt ttaacgtatc cataattatt cgttcactaa ctaagtctat
     9721 ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac atcttgctaa
     9781 aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag aatattttcc
     9841 catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt taattctttg
     9901 aaaaactttc attccggcat atgttcccat agatacacat tcttttactt ttattggaaa
     9961 agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt ttttattgat
    10021 atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca ttgctttaag
    10081 caatgtggat tttccagcac catttggacc aataatacct gttaaagcac cttcttgtat
    10141 ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa gctttttcac
    10201 ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat ggtatacaaa
    10261 aattatattt tagtcaaccc taaattttat agtttagatt tttttctgtt gctgaagatt
    10321 agaatgacaa aactatttga aactacgcgt atagtaagta gctttcaaaa tatataaaaa
    10381 aggagcgttc tcaaatgaga acatgctcct aaaactactt tattggcgta tggaaaaatt
    10441 atacttgaag tggatagcgg gttgaataag ttgataagca tcaaaacatt tataaaaatt
    10501 attcaagact atgtctgttc catatacaaa tatgaaaatc agtaaactcc atttaacttt
    10561 tttcgtagtg tgtcttttta tacatgttta tcgttattaa tagtgaatag gaaaaaaact
    10621 atttttgatc attgacaatt aaatacaatt tttgaataac gatccctgct attcgagcta
    10681 gaaactcaag tacgccatga cctgattagc gacaaatact tagagatttc tttttgttct
    10741 gggaaaacaa aatgccatga caatagcagc aaataatgat acttccgtaa ggctcgccgt
    10801 aaactgggga gaggtgaaat tcctatgtgc ttacccaaag caattcaaaa atttcataac
    10861 tactatttta ggagatgatt ttatagaatc cagagtctac ctgaataaaa aacaaacctg
    10921 tgcatttcta ggaatcgata ataatactct agataagtgg attcgtatat ataatttccc
    10981 agtaataaga gttggtggtg tagttcgctt tgacgtagaa gatattaaag actttatgaa
    11041 tacatttaaa ataacttgaa attttcttct cgccgcatat actaaaatta gtgaaatata
    11101 ctgccttact ttttttaagg agtgaggatt ttgcctacaa agctttcaaa tgggaaatat
    11161 aaaactaatc tgagatatcc aaaaaggttt aaagaaataa caggaattgc atcagaaaag
    11221 tatcaaaaaa catttccaaa taggcaattg gcgattaaag ccgaaaatga tatgaagaag
    11281 aaaatagaaa aagttttgag agaggagaat gcaaactcat tagaattaaa aggaaaaata
    11341 acgtttaaaa aattttacga atctaaatgg cttcctagat atgaattagg tcaaacaatt
    11401 agatcaaaca ggcctcctag tgatataacc attagtaata caaaagatat atttcggttg
    11461 catattttac ctatgtttgg tgaatacgca atgaattatc taaatatcaa tactgagata
    11521 atatcagatg aattaaccaa aaaatcgaaa gaatatgcaa atataaaaat cataaaagga
    11581 tatgttcgtt ccatgttcga tattgcagaa atactaaatt atatcgaatt taatcgaact
    11641 actaaaatta ttcaaagtat cactgcacct aaaaaaaatg ctttagaaga aaaaagaata
    11701 caagaaggta agcaagcact atcatcaaaa gaacttacta attggataga agcagtaaat
    11761 gatgatttta ataatcattt acttactttt catgattaca cactttttat gattaccctg
    11821 tatcttggtg atagaaaaag tgagacttac gctttgcaat ggaaatatat tgattttgaa
    11881 aaacaaacgg tccgtcttaa acatacttta gataaatatc aaagaaaaaa gtttacaaaa
    11941 ggtcgaaaag ataccgtcat tcaagtacca gaagttgtaa tgacattgct aagtgaatgg
    12001 aaatcggtac aagctgatca attgttgaaa ttaaaaatta agcaaacgtt agatcagtat
    12061 ctctttactt atactaaacc atctggagaa gtaaattgtc ctgttcatgc cgattaccta
    12121 aactatcgaa tcaattcaat taaaagaagg catcctgact tagttcattt atctccacac
    12181 aaactccgtc atacttatgc aactatagct agacaaggtg gagcagatat gaatcaaatt
    12241 tcaaatgctt taacacactc agatatttct acaacaaaga tatatgtgaa cacaccagat
    12301 atagttgata aggctgtttt tgaagctttt cagagaggat tgaataagtg tgattgatat
    12361 gtgttcttaa atgtgttctt aaaaacaaaa gaagcacttt ccaaataatt gaaaagtgcc
    12421 agaaacgttg atatataaag gatattaacg ttttgagaat tgtgaagctt tacgtgcttt
    12481 cttaagacct ggttaaacaa acttttcatt tataatgttt tacagagata tatgtcgtat
    12541 gaaattaatg ttctcttgtt catgaagttt tacagaattg tatagggtta tctgttgtca
    12601 ttacctcaaa tcattaccac aacttggcga gttggctgat tgaagaagta ctctgtttta
    12661 ctctatctta ctctgcttta ctctatctta ctctgcttta ctctagctta ctctgcttta
    12721 ctctagctta ctctatctta ctctatctta ctctgcttta ctctatctta ctctgcttta
    12781 ctctagctta ctctgcttta ctctatctta cctgctttac tctatcttac ctgctttact
    12841 ctatcttact ctgctttact ctatcttact ctgctttact ctatcttact ctgctttact
    12901 ctatcttact ctatcttact ctgtcttact ctatcttact ctgtcttact ctatcttact
    12961 ctgttttact ctatttaaag aatagtattg agtcggatct ttggtactag taccatgcca
    13021 ctgaattatt tttttcctta acatttcctt taatacctta cggctatatc cgaccccttt
    13081 ttttaatact tctgaagctt cttttaccga aattttttcg ttattaaata aatgtgaaac
    13141 aatcatcttc tcagaagtag acaaagagtc tacttcaaat tctccaagat aatcatctag
    13201 tctgtctctc tctcttaaat gtctagaggt tatactattt tctaagacta ataaaaccga
    13261 attattattt ggctcactat atttaggttc atttaaaaat agtaattgca tttcgtcata
    13321 tattcgattc acaccttcat taagttcctt aacccatccg aattcagata atgtcctagc
    13381 gattctggga ttcctagcat acctagttcg gcgcatattt tgtaaagtga ctatattagg
    13441 taaagctccc gggctaaaaa tttctaacct atcatcatac atattaattc taacgtaatc
    13501 ccctttataa gaatagtctc tatgcgttaa agcattgact ataccttcaa accatgcaaa
    13561 ttcaggatat tcaggtatga tactaaactt tccatcatca tttaaatact gaaattctct
    13621 tagttgcgtt tttacatccc tagttataga ttcgattagc tttggaattg caccttcata
    13681 gtcaaattct ttaactatat ttaatctccg tccagtttca ttacgcttac catcatatct
    13741 caaaaaacga attctagaat taggtaaggc tagaaatgta ttcttaccaa atagaagcat
    13801 gccagcattt gtcaagtgac catttctcat taaccctctt ccagtgagta tggtttctgt
    13861 ggatatcgct tcagcttttt ttaatttttt atatgaatcc atgagagatt catcaatatc
    13921 atcaagagta ctgtctaaaa tttctatatc ttcaaacatt ctctgtcctt tgtcgtattc
    13981 tagtttggat atttggtcat gagtttgatg aacactttta tccccaattc tcaagaaaca
    14041 atcaccgttc gaattaaaaa tcacatggct tatgctaggt tcaacgtcta taatgaataa
    14101 tatatcgtct tctcctctag cattcttaat actcttcatt tcttttttaa aagttggatt
    14161 gggttttgtc attgaattta tagctagtac atattcatct ggatcgatcg caccttgaaa
    14221 tttaaaccca gaaatagtcc catcatcttc tataccgatt accagcttac ctccatcggc
    14281 atttgcaaac gctactatgt gttttataat atctctaggc ttaatccgtg cactttttct
    14341 atcaaaatac tgatcttctt tttcacttat aaacttttga atctcataat ccatttaaaa
    14401 tttcccttct taaatttttt tattatagag ccgtatattc aatttccaca aaaaaataat
    14461 ctaaaacaaa acagacacct tcattttgat atgttctccc acatatgaat cggtcgctat
    14521 acagcattac atatagtaat tattcatact gcgaaataaa tagctccaaa tctttagctg
    14581 gcgtagttaa gcttaaattc gaaatgaaag tttcattgac tattccactt tcatcaaatc
    14641 ccttcttcaa ccaatcagag ttccacaacg taaatccttt aggattagaa actttcttac
    14701 ataattttgc actataggat ttctctcttg aatgaattaa agaaagtctt tgtaaccaaa
    14761 tttcaactat atccgtatta ggagtattaa caaattttct taagatttta tccaagtata
    14821 agttgatttc ttctacagca ataaactcaa aaagcttacc taaaatcaca atacaagcct
    14881 ccaaagttct aggattcttt tgcattatat taacaagaat actaatcaat tggtagatat
    14941 catctggtct tttgtttaaa gtagaaactc tatgtgtata gacttcactt agcgctttac
    15001 ttaaactccc gcaatttgga tatttgtctc ctaaaagctt tacctgtagc aaatgctttt
    15061 gaaggctaag cttaaacttc cacttattat tacttttctc tgtaaaactt gcatgtttaa
    15121 gatcccagta aattttatca ggcttaattg catctaaaat tatattatcc gataagaatg
    15181 ttttatttga atttagcttc aaatttagat ccgataatac ttcagataac aatctgataa
    15241 tcacttcagc ttgatctttt cgaaccgaaa aaattctata atcatctctg taacgaagta
    15301 ttttgaactc tttaatctct gttttttcta tcttgttcag taacatttta tcagcatacc
    15361 caagtactat ttcagctaca aaatccatca atacatttcc ttgaggtata ccgttcgttt
    15421 gtccattttg taaatatcgt attttagaat ctattaagtt tccaacgcct tgagtatctt
    15481 ttctatgtgt cttagcccat tcttttgaat gtactgccca cgggatagta tgtgtgtaca
    15541 ttgagctata acaatcggta atatctgaat gaatgcagta gtcatactcc aaagcataac
    15601 taatctgtgc ttgttcaagg ttctcccacc aatttaaaat agtttccgca gtatcgctct
    15661 ttgaagaaat agattccaca ggaagtgata tacattcaat tcgcttgtcg gcttggaatt
    15721 cttcaaactt atttttgatg tgattccaat tatttttctg tgtaatcccg tttactaagt
    15781 ctacatatac taatggatgt aatatttgaa ggggtctcca agcataatta ccatctttat
    15841 tagctaatat tcgatgatta acatcaactg cctcagagag cgatctctta tgtctaacta
    15901 gttcagtaaa tgatttgttt tttaataaat ccactgcttt ctcaatgcct actgataaat
    15961 caatataatt gggcaacgat gtactaaaaa aacttgatgt ttttaaaaag aattgctttg
    16021 cttcttgatg attcatctct aaaatacttt ttctagtcct agtcaaattc aaaatctcct
    16081 agatcaatta agttgtaatg taagaggata ccctaacgtt acatatctca ttcattatta
    16141 taccagtatt tcttcatgac acataagggt gtaacataat aattttaaac gaaatgacta
    16201 caagtagtca ttgaagtaag tcttcataag atacctgtag gatatcccta attgctctca
    16261 attgatctaa cttaatgtgt tgccggccac cttcaatttt aaccaatgtt tccctagtta
    16321 tagcaatttt atgaagctgt aggtctctaa tcagctcggt ttgaccaatt cctttttcta
    16381 atcggattcg tctaatattg ctaccgatta cattttcttt atcaataatt ttttcctctt
    16441 tcaagaatag cacacctttt ggactaaaat tagtcctttt tcttattaat cttaaagatt
    16501 cggtatgcta caatgggact agtattagtc cacattcaat tttaaggagg aatttttttg
    16561 aagaaatttg caatcattct aacctgcgct ttactcatcg gaactataag cgggtgtacc
    16621 acttctgaaa caagttctac aagtacggaa actagtacaa caacctcaga aaagaattac
    16681 ccaatacatt tttttccggt tccagatgga tttgaagctt attatcatga taaaaaacta
    16741 gatgtccctt tatcagaaac tgaatctact gaaaagattt tgccaattga aattgaaaga
    16801 aatcaagacg atataaaact aaaagctgat cttcccttac tgaacagcac acaaattgat
    16861 agacatgaaa atattaaagt taatattgaa aaaggagcgt tagcaaaact ttatactgaa
    16921 aaagttgaac aattttttaa gtctggtgcc aataaagact tttcaaaaat aagcgattat
    16981 tcggagaact tttttaatga aacccaagat gaaatcatga actcaacttt tatggctgaa
    17041 gatatgtaca atatgacatt aaaaaaaata aaagcttacg accatccgtt aaatctgaca
    17101 ctaaataatg gactgctttc ttttgtatta gcaggttacg ttgactatga atattcacca
    17161 gattatttgg agaaacctct tcaacacgag aacaatgata ttaattttag atttgtctac
    17221 gagtcaaaaa gtaaaacatg gttagttgat caattaagca cgataaacac aagcttccat
    17281 gaattaccta aacctgaaaa tgaagttgag atggtcatta aaatcttcta aaaaagaagc
    17341 gttgaaggag aaatattcct ttcaacgctc ttttttatat gcttagaaac ataaataaat
    17401 tgtatacttt ctttttagcc cctattactt ttcacgaaaa atatcttttt ttcgacgcta
    17461 gcgtttctct acttctaata ctgtacacag tttttgtatt attgaaaaat tactcattat
    17521 ttagcattaa tccacatcgt tttatcaatc gaccttcata ctaaactact ttaaagtcat
    17581 cttccttctc gtttttcatt aaatcagaca cataagccca tgtacccgtt cgttgtaaat
    17641 aaagttcttc ttgtaacaac tcccattttc tcttagaaat cataatgacg ccatcgttca
    17701 atccattttt agaatcccta gtaatttcta tttcagaatt tgaatgaact acttcttgca
    17761 acactttacc tatattttct ttgaagtttg ttggagtaat agtatccaaa atgaacacct
    17821 ctcttctcta tacctctatt atgaagcgcc catcaaataa agcaagcgtt gaggaataat
    17881 tctatcaaca cttgctttat taagctttca ttttgtagtt tgactaaaac caataatctt
    17941 atttctaaac tctcgaaaaa cttcttgtgg ctgataatag tctgcatcta tttcaaatgt
    18001 ttctgagaat aattcattga atttttctac tgaaataatg attttaccat tcttctctcg
    18061 actgttttct attatttctg cttctaattc aaataaatct agtgacgaat taaaatcata
    18121 aatcctgtca tattgaacaa aaaaatcaag aaaaagttgc tcaaactctt tcttatttat
    18181 cactagtgtt tcaagcagat caactttatt ttcttcagtc attcttccaa tttcctcact
    18241 ttcgttttca aaaatcacaa aaatgatatt aacttaaaag ccactaacaa aataagactt
    18301 acaaattaaa gggtaatcca acaaaataac cattcgctct acgtactacg atttggtagt
    18361 cctgcctttc aatcggtaat tcataatagt aatttactgt ttctgattta ccactttcaa
    18421 tatactcagg attatccaat atcatagcat actcataaag ctcttcacca tccttatttt
    18481 caatcgacca ttgttgcgga cgtatcggat tttctgtatc atttttcatt gtataggtca
    18541 cttgtaatac acgatcatgc tgtgcaaatt catttctttt ttcggtcagt tcaatatttt
    18601 taaatgttag ttcaacccca tctgcctgat aagtttcacc tatttttaaa tcttttgtta
    18661 caatttttat tggcttttct tctggaaaat acctggcttt aattgttaga taacttccaa
    18721 aaagaatcaa tcctagtagc acaaaaaaaa ttcctttaac gagccactga aacgggaagt
    18781 aaactttaaa catctttttt ctcctccgtt ttgtatttgg tttttagaga acacgataac
    18841 ttttgatcaa taccatccaa ctttttcatt atacttgatt ctagttctga aattgtttgt
    18901 tcaagtttaa tgctttcagg atcactaaat tttttatttg attgactagc caacattggt
    18961 aaatttgaaa ccatcacacg gaacatattt gctttcgttc caagagttcc tttattcttc
    19021 tcataaaagc tgtcaaacaa tttttttgtt tcctttgaac aatatacttt aatatacgga
    19081 tcagacattc ttttccctct ccctgtttac ttaaaaatcc atatcatatt gattttgttg
    19141 tttttgataa tcaattcctc ttgctgtttc ttcatactct ctccgagctt tttcaatcgt
    19201 accatcatca attgcacgat taaataaata aagaacacgt tttgtttctt gaatataacg
    19261 cttcttaaaa ttacgtttta aagcgggttc agtatcttga atgttaaatt ggtcataatc
    19321 agttgataaa ctctctgcga tatcataaac aactttttta tacaccaaat ttacacattt
    19381 cttcttatat tttaaaggtt cctcttccac tgcaaatcca aagaattggt ttttattctc
    19441 accaaaaaca tccaataaat tttctattct gtcctctatt tcaaatttat atttatcata
    19501 aaattcttca taatcttttg atgatctaag aaatggtata ttctcattat ctaattcaaa
    19561 gcgttgcgct cgttcttgcc aattaaatct actatccgct ctaccattta acgatttacc
    19621 aagatgtcga atagcaattt cagtgagtac ctctttagat tgaatttgct ccgctttttt
    19681 attttctgtg aacaaatcat caagataatt catatcatag ttttgcccta aacgctttcc
    19741 gccaattttt aagattaact cgccttcaga atctaagagt ttgtacccaa tacgctgatt
    19801 tttggcaaac cggtaagtct caatagaatg gtttttcttc aattccgcat gaaaatcatc
    19861 ataatttttc gcatactgag caacttcaga aacagctagt tttatttttt ctcgatttga
    19921 aatcttattt tcagttctaa attcacgttc tttccaattt tcacgatctg caactaaatg
    19981 attatactta tacatatcaa gtgggttaat tccattatca ataagtatat tatcattcca
    20041 agcctgcaaa actcgaatat cattctttcg aatatccatt ttctttcctg ttagctgatc
    20101 gtttggcgaa ataccaaagt gattgtgaac atgccctttg tctgtgtgtg tgacaaccaa
    20161 aattgactgg ttaggaaaca ttttttctgc ccacttaaca cccaactcat gtgctttttc
    20221 agcggaaata tcttctagtg gatcaaagct ttgtgtaaaa tgccaaagct ttattttccc
    20281 tttatcacca aaaatttcat tggttcgttt aaattcctca atagcattta aatcattcga
    20341 tacgccaatt cctgtcacga gttcagaatc ttcttgttta tttaacatat atgaaattgt
    20401 attatgaata ctcctacttt ttgaagcaat ttttataatc gagcccataa cttattcaac
    20461 tctttttcca agcgattcaa tggatcgctt attgatttta tttgtatcat taagtcattt
    20521 ttttcagcat atagccgctt taatcgctct tgcttagttg taaaagataa gtaaacactc
    20581 tgatgttctt ctttttgtat ctcggtatct tttttattta actgaacacg caaataattt
    20641 gctgttttca ctaattgatt caaatttctt cctattgcta gtaaatgaaa attaatttct
    20701 ttatattgtc gatcggtata ttttagactt ttccctgcgt tgtctactgc gattttcttc
    20761 acgtacattg gaacagataa accaaaaatg tctgctgttt tttctagctc ttggtactga
    20821 taatcgtcta ctcgaaactt tatcggccta ttttgtcgtc gactatcttc attacgttta
    20881 tatcgctttc gcctttcttc taatggtagt ttactgacat caattgccat gcgcacttct
    20941 ccttttcaat attattttta agttcaaaag aagcaagcga ggggaaaggg aacccttccc
    21001 cgcttgtcga gggagtaaac tccctacgag caccctcatg ctccgcattt tatcgagatt
    21061 agatagccga taaatcagct acctaacact ttcttcttgc gcctcttctc gatagaaaat
    21121 tcgatacgct gtctctcctt ttcttctcaa aaattgttgg aatttcgttt ccatcaattg
    21181 atcgcttgca agaaattcgt gtactagggt aaattcgcta ttcgcgaaat ttaccgcctt
    21241 ttgccatttt tcttgagtac ctttttgatt aatttcaaaa gtttcttcaa actcttttac
    21301 tgatgaaatt cctgtttttt catagacata ctttcccagt tcgggataaa gttttctctc
    21361 caactcggcg agttcttttt tcaaatctgc aattgacttt tgttttttcc ttgccatttt
    21421 tcatgctcct ttttgtatgt gactaagttc attcataagc tcatttttca tcattaattc
    21481 ttcttgaatc atcagttcaa tataatttgt gaccgtgatt ttttcatttg ttgctttctt
    21541 tactgattca cataattcgt caagttctaa gaaatcatgt gtactaatct gtagaattaa
    21601 ttgcatgaca atcacctctt tattttttaa atagactatc aaacagcgga tttggtttat
    21661 cagaaatatc ctgaaacagt ggattatttt cataagtaaa tcgttgacgt ttcttctcct
    21721 tcacttcttg ttctacaatc actttttcgt taggtctttc ttttcgttcc tcgacaatat
    21781 ttattttttg cgtcttatct ccctgacttg cttcagctct tgtttcacgt acttctaccg
    21841 tttttggttg atttggcgca ctactttgta cattttctcg tacgtctact gttcgatttc
    21901 cactttgact acttgtagta tcctgtaagt ttaatcgaac atctaccgtt cttgtttgac
    21961 ctgtactgct actttgcaca ttctctctga catccactgt tcgatttcca ctttgactac
    22021 ttgtagtatt atgcaagttt tctcgaacat ctactgagcg cctatcttga ctacttgtag
    22081 aattttgtag attttcttta accgattttt ctaatccggt atttgaattt ttagcttctt
    22141 ggattctttc tttggtcaac gcttcttttt cgtccatatt agctcctgta cgcccttcta
    22201 cacgtttcct taacatttcc tcacgattca ttgcttcagg cgtgtatttt gcttcctgtc
    22261 gcttctgatt aattaattct tcagcagact tagcttgacc gtgcatagca tttttacgtt
    22321 cttcaatatc ttgttgaact ttttgctgaa ttgcacttgc ataattagta tttgattcag
    22381 caatattttt cattgcttca cgcatacgtt cagaactaga cataattcta tcggcattct
    22441 caatcttagc cccatctttt ctatccgaaa gacgtattgg catagaatca gcatattttc
    22501 cagtttcatc ttctttgttt ttagctacta taggtttgct atttttatta atagcggaca
    22561 cggtaccatc atctaaacct tctttgaatt tattcttggt atcattagcc atatcacgca
    22621 tactcttaaa tggttttgtt gctctttcac tagccattgt agctccatcc gaagctaagc
    22681 ctgataatcc acgttcacga gcatacccaa gcgttttccc cgttttatta gctttttgag
    22741 aaagtgattg accaaagttg cgttcaggtt ttcgttcttc cccttccgtt tgatcgtttc
    22801 caccactatt tttcccacct ttagatcggt tcatgccacc tgttccttta cgaaacagca
    22861 ttgcacccat tccaaaagca gaagcaagtt gcccatagcc ttctttgagt ccaatatcca
    22921 caccaaagta acgcatgact gttccactgc ctttgattaa aacaaacaca gcacagatat
    22981 aagcaattgt ttttataaaa atatttgttg agataccttc gccagtattt aagtaattta
    23041 tgaacattgc aaacatagat aatccaaacc cttgaaatcc gactgtcaaa tagcactgta
    23101 aaatatctga caaaacttgt tttgatcgtt gtcctgtttc taagtcagta gcaaatacta
    23161 aaactccgag tacacgtttg aatgctagct cgagtattgc tgtaattata atgaaagcac
    23221 taaaaacata cgctaccgct aaagcaatca atccaaatga aattccccac aggttcgctt
    23281 gatagcgata atatcctgat ttgataccat caacaaaggt tgacaaaaaa ttatctgaaa
    23341 atttagtagc aacaaattca ttatcagaat tttcaactaa ttcataacgc aggttatcag
    23401 ctgttgagtt ctctttttcc attttatcaa ttacattatc tgtcaatact tgcgtaagat
    23461 ccgttttcca aaaattttct tttgttagat tgttccgatc attcgtttta tcgatcatgg
    23521 aatattgatc cgttttattg atataagcta aatcagtaac cccttgttca atcagcgacc
    23581 atgccacacc gccatcttga ttgttaatac tagttgcgtc attataaaga gtctttgcaa
    23641 acattattcc tgaaagtttt tctgttgatc caatcaaagt cggcattaaa agaattaata
    23701 ctatcgacat tacaacattc attccaacag attttagatc aactgttcct tttccagtca
    23761 ccattttata accaataatc actaatgaga gtaccattaa accaataaca actgtattta
    23821 aaactgactg atagactttg ttcaatccag cactttccac aaagctaaac aaatctaaaa
    23881 cctcgggaat catattttga atgccatcga caacagcgaa cattgatttt ataacagacc
    23941 aacctatcca cataccgaac tggctgtaaa aatttccgtt atatttttta agataattct
    24001 gataaagtgg attagttaaa aactcagttt gactctcagt atcatcaaac cagttgaaaa
    24061 tactgccttc tgcaacgtta ctcgtaaaaa taatcatagc tatgagaata agaaaactaa
    24121 tcaacaacca atttattttt ctattatcaa tctttttttt aattattttc attgttacct
    24181 ccctcagccc atttttttag gctctcagaa agcttctgag ccattttttt attaacgatg
    24241 atgtgagtta cgtaatcatc attaacagct cttaaatcta gaacaatagt atttgagcag
    24301 atactctcga ttaactcaag agtatctgcg ttatgatcaa gacagtaaat cttatctact
    24361 agatgatttt caatcaaaat aacccctccc gtagtcaaag ttctcaattt tatgaagata
    24421 tccctctact tcatttaaat caattctatt cttattgctg actaacccgt tgtaaaattc
    24481 tttttgtaga ttatatttat ctgtttcttg aatcttttta tataaatcaa ccagggagtc
    24541 cctgtcttta aaaacttgct tgaattgttc gcttgtatat cggttttcat taattgcaac
    24601 aactgcttga atttcaggcc atttttcttt caacttaaaa taacggggaa cgctgaaata
    24661 gttctcaaaa aagaagtctc gtgcttcata gtattctcta ttttgtcttt ttatatcctc
    24721 gacaatttcg tatcgtgtag agatcttcaa agctgaactt tcgttaattt ctgttccttt
    24781 tcgaaagact ttcacttttg aagggcgttc aattttataa tttatcccct ttttacttga
    24841 ataacttttg atacgaccat caaattgaac catatctcct ttttttaacc acccaaattt
    24901 tagaaattgt ttagttaaat tcatccaaat gtggtctgtg accaactcta agttatcccc
    24961 ttgaacaatt tttaaattcg ataacataaa agtagcattt tcatactctt gaccaaactt
    25021 gtatttatcc tttttatatc cgttaaattc aaaggtacct tgaaatgtat gccgtttctt
    25081 atctcctaat tttttcaatt tttctctcaa aataatcaac tccatcattt aatatatttt
    25141 tgtgatggta gatactctcg attaacacaa gagtatctgc gttgtaatca agacaataaa
    25201 tttgatatat taaccgtttt tcaatcatat tattaatcct ttctaaccta tttttttcag
    25261 tgtctctagc atatccagtc tactagcttt ttcttgctta gctatgtcct cccagtggtt
    25321 cctagctgtg gtgttgataa acttcatttc atcaatttct tcaccgagat tcagtttttc
    25381 atttatagaa ttaaaaagtc ttgttagcat tttgtttgtt atatcaaaat agtcttgacc
    25441 tcgaatttct attttatatt ctctttcaat tgaatctttc atactagcaa tcaacttaag
    25501 atacttttct ctttgaactt cagtatatct atcacctagt ctggtaatta tttgaattcc
    25561 tttctgattt ataatatttt caaaaaagga attctgatct tgattaatct cttctatact
    25621 atccttatct tgattagttt gatcttgact attctgatct tgacttaact gtgtatccga
    25681 tttgacgtcg catggtatac cagctggata ccatttttcc atcagatcaa ctcttaatgg
    25741 ttctacttga ccagactgca aaatcatatc aaattttatt ggctttagct ttgagaaaaa
    25801 ttctggtttt aagttcaata acttgaatcg ataaatagaa ggtttatacc tatcgattcg
    25861 tatattttga ttttgtaacc agtggggtag aaacgctaac aaatcttcat ttaaaaaaaa
    25921 tacaaaacct tttgtaaaaa gtatctttac atcatcatcg ggtgttccga gcattttcgt
    25981 gtatggataa gcttcgacaa ttccgtcatc atctgcgttt gtgttcagat atacgtataa
    26041 taattttgct gaatctgaca tcttcataaa acttgcatta tttaaaaatg atttagccag
    26101 cattcgtttt tctgccataa aaatcacact tcttcctatt tgaatgttaa aatgtagtca
    26161 aataaagttt agctagaact ttattattga gcgttcctag tggtctgcaa actaacggga
    26221 acactctttt ttattcttat taaaaccagc aatactagtt caccttcttt catcctttta
    26281 catccaatta aatttgatgc tgtagcatcc atttttttac ctcttctttt gaatatagtt
    26341 caattgtgtc aaccacgata ataggtaatc ctaacctttt aaacttcaga aaagttccat
    26401 atgatacgtt tagaaatttt gccatttctt ttcccttaag aaaaggtcgt tctaatccgc
    26461 tgtctattct tgcttgatca atagcttctt ttgtcacttc aaacacatgt gtcttcaact
    26521 gatttgtttg atcagtattc aacattacac tgactccgat ttgctccatt aaaaattccc
    26581 actttcatat tttcatcaat aagccaactc gccaagttgt ttgttaataa tctgatttca
    26641 ataaccaacc tattattttt tgataaacat ttttcgacac actttgaact tcgaagcatt
    26701 caattttttt aagagtttgc cttgaaattc caattaattc tgctgcttta gttttcgtaa
    26761 tcatcaactt tcctcgtctc tctcgaattc ttatactaaa atcctcatta atttgaaaca
    26821 taaacatccc tccattttta tttttgcatt tatccgttta tccgatgtcg gataaataga
    26881 ttttagtata tattattttg atagtcaatt aaaaatatac attttggata aaattgttca
    26941 tttttttatc caaatcatat attatagata gtaagaggtg aacttcattg actcaaaaaa
    27001 acagaattaa agaaactcgc gaagaacaga atttaacact taaagaactt agcaatctgt
    27061 cggatattcc gtatcaaact ttgagaaact atgagattgg taaaagaaaa cccagaaatg
    27121 atgaagtttg gtggaagtta ggtacgattt taaacgtccg tgggccctac ttaaaaggag
    27181 aagatagttt taaaacaaat gatgaattgc atatttatta tctaaaaaaa agtcatacaa
    27241 attttgaact gtctaagaaa gaagaaatta tagatataat agataaacta aacgaagacg
    27301 gtttagatga agccttacgg cagttagcac tattatcaaa aatagatgat ttcaagcggt
    27361 aacttactta tcatcatgta gactatcagg tatcataaac taattcctca atcagccaac
    27421 tcgccaagtt atcagattgg agagattagt tatgtcaata caaaagtaca aaacaaaaag
    27481 cggtacacga tatagtgtcc gcttatatgt tcgtaaagat gaaaatggga agcaagaata
    27541 ctatgtaaaa aaaggcttca aaactgaaaa agaagccaag ttacacgaag caagaaaaaa
    27601 ggtcgagatt gaagatatgg gctatcacag accttctaat gacttattta gaacagtcta
    27661 tgaagaatgg ttgccgtctt ataaaaatcg tgtacaagaa acgacttatc aacgaacaaa
    27721 agaccttttt cgcttgcata tcctgccaaa gttcggagac aaatcgattt ctaagattac
    27781 tcctatttac tgtcagaatg caatcaatga atgggctgta aaatatacaa atatcaagca
    27841 acttaaatcc tacagctctc aaatatttga gtatgcaatc ttcgcagaat tgttacagcg
    27901 taaccctatg acaaatacca aactgccaaa acaagaaaag aaagaatccg aaaattattt
    27961 ctcactagaa gaattaaaag agtttttgga aattctcaat aaagaagagt ctttaaaaca
    28021 tatcctgatc tttcaacttt taattactac cggtgttcgc aaaggtgaat tatcagccct
    28081 acgctgggca gatattaatt ttgaagaaca gttactacac atcggaaaaa gttatgcaac
    28141 aattcgcact gaagatccga atgctactag aaaaacaatc aggattcaga agaatacaaa
    28201 aaatgtctct tccgaacgaa ttttaccgat tgatactcaa accatcgaaa tgctgaaact
    28261 ttggaaaaaa gagcaagcag aggtactttt acaattcggc attaatgcaa ataccaaaaa
    28321 gcaacttatc tttacctatg taaatgcaaa tggcgatgtt aaccaacctt tacacgcaga
    28381 ttattccaat aatattatga gacgtttaga gaagaaatat aaatttaagc acgttactat
    28441 tcatggattg cgacacactc acgccacttt acttttagaa ggcggtgcga gcattaaaga
    28501 agcacaagat cgattgggtc ataaaaatgc tgaaactacg ctcaatactt attcccatgt
    28561 aactgaaaaa gcgcaacgaa atgcggtaga caagtttatt gcttacactg gattatagac
    28621 aaaaaagttg ccgtcattac ctttttcatt accacaacga aaaaaagcac cttccaactg
    28681 aatggaaagt gcttgaaatc gttggtataa caacgattaa cgcttagaga attgtgaagc
    28741 tttacgagct ttcttaagac ctggtttttt acgttcgacc atacgtgcgt cacgagtaag
    28801 taagccagca cgtttaagag ctaaacggaa gtcaggatca acttctagca atgcacgagc
    28861 gataccatga cggatagctc ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa
    28921 tacgtcatat gcgccttttg tttcagtaac agcgaaaggt tgattgatta cttcacgcaa
    28981 gtcagcatgt ggaatgtatt cttcaacatc ttttttattt acagtaattt tgccagttcc
    29041 tggtactaga cgtacgcgag caactgcgtt tttacgacgg cctgtgccga tatattgaac
    29101 ttgtgccaat gaaattccct cctattagat taagtttgtg atgtctaaaa cttctggttg
    29161 ttgtgctgca tgtggatgtt ctgctccacc gtatacgttc aattttgtaa attgtttgcg
    29221 gcctaaagtg tttttaggaa gcataccttt tacagaagtt tcgatcaaac gacgagaatt
    29281 tttagcacgc aattcgccag cagtgattga tttcaatcct cctgggtagt tgctgtgacg
    29341 gtaataaatt ttgtcagtcg cttttttacc tgttaatttc actttgtctg cattgatgac
    29401 gatgacgaaa tcgccagtat ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag
    29461 tacagatgca acaactgttg aaagacgtcc caatggaaca tcagttgcgt ctactacata
    29521 ccatttacgt tctacttcgc ctggtttggc catatatgtt gtacgcacgt tattttcctc
    29581 caatttaagt tctgatgttt gacatacact tcgagtttcc ggggctcttt gtggggcaaa
    29641 caataccatt taacatatta cccgctaatc tagtcaatgt caacgttttt ctgcttgatt
    29701 gaaaaattta tatgaaactt tacttctttt ctttctttta tataaaaggg tcgtttcctg
    29761 tcaaaatgaa aaacaaaaag aggctgtgac aaaagtcttg tcacaacccc ttattttgaa
    29821 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccata aaacataaga
    29881 agctgttttt taaaattgtt tcttgtgact cggagctgaa cggcaggctt acaacctctt
    29941 ggtaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    30001 gaactatttt gacgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    30061 ttttcggtca agttcaacaa cagtttttac gcgtcgatct ctggcgcttt gcccaaatga
    30121 gcttggatca tccagatacg tttttctgtg gctgttttga attcgataaa gatatcttgt
    30181 gttggatcgt cgccttcttc tccagaaact tcgattcctt tttggtaaag atctgctaga
    30241 taacgatagc ctgcaacaag tgtttccaaa cgttctggaa tagtacgatc ccagcggcca
    30301 atttcatcag ggatttttgt gttatctgca aattcacgca atgtagaata aggtgaaccg
    30361 tctaatgtga tcaagcgttc ggatacttca tctaactgag cgttcaaatc atccatgaac
    30421 tcatccatca ttgggtgcaa tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg
    30481 tgaacaacaa cagacatttg gcttagatct gcaactaatt gatttaatac ttctttggtt
    30541 tgttggaatt tcatctttct tgcctcctaa cattttctta cacttaaaat aatagtagac
    30601 tttcaatgaa aagtacaaca aaatcattta cctgagaacg attttcagtt cgctttttta
    30661 tccgtaaagc caatcatttt tcagaatcag ctaattcttc tcgcagttga gcaaattgct
    30721 gataagtctg tgctaaatgc ttttgtgtcg aaacaaataa tggatagatt ttctgatatt
    30781 ttttatgatc agtttcttgt actccaactt gctctttcaa ttctatcaat tcctgtgctt
    30841 cactgatact ttggatcttt ccgatgcttc tccagcctaa aatcacagct cctaatgcgg
    30901 atgcttctgt gacagtaggg aaaacaagcg ggtaacccag tacatcagcc atcatctgac
    30961 gaaaaatcgg agattgagca aacccgcctg tagcacggat ctctgtgagc tgattttcct
    31021 ggcaaactcc ttttaaaatc cgataaaggt tgagacagat cccttctaga atcgccttca
    31081 tcatttcttt ttctgtatgt tgccgctgta aaccgataaa actgccaaac gcatctgcgt
    31141 cccaaagcgg tgctcgctca ccaagtaaat agggatgaaa aatcagacca ttggcacccg
    31201 ctggcacaaa agcgatagcc gaaagcagtg catcatatgg attttctcct ttttctttag
    31261 cagattgaac gacttcctgt aaaaatgtat gccctgccca atcaaagatg ccagcaccat
    31321 tgctagtcgc tcctcccact acccaatgtt tctgatccag aacataacaa aacgtctcca
    31381 tttctggatg gaactgaggc tgagtagtaa taaaacgaag tgctccgctc gttcctacgg
    31441 tcaaagcggc gatcgtatca ccaaaagcac ctaaccctag attagaaagc ggcccgtctg
    31501 cccctcccaa aacaaatgat atatcatttg gcaaacctag ttctgctgct gtttttgatg
    31561 gtagacctgt tacttgatag gtggaaggaa agacttgcgg caattgctct attcgaatgt
    31621 tcaagtagtc aagcacttct tctgcccatt ggaattcatg gatatcaaaa tatccagttc
    31681 ctgaagctgt tccgtagtcg ctgatcagtt cgccaaacaa tctataaaag atatagtctt
    31741 tgataccgac aaaataagca gtttttttga ataattcagg ttggctgttt tgcagccact
    31801 tgattttgtt caatggtgtc attggatgaa tcggcgtccc agtctgccga taaaattctt
    31861 tgccaagcac ggtatatttc agtttttctg cttcgtccgc tgctcggtta tctgcccaag
    31921 tgatcattcg agataacggt gttttctctt catccaacaa tatcaggcca tgcatcgcag
    31981 aggagaagga gacaaattga atgattgtgt ctgaaggcaa ctgtttaatt acttctttta
    32041 taacggtgca gacaccttga aagatcaatt cctgatcttg ttctgccatc ccttgtgttt
    32101 cttggatcaa agggtagcga aaataagcgg atgctgcttc ttgggcttca tctgtgaaag
    32161 caactgcttt ggtttgtgtg gttccgatat ctaatccgat tgaaacgttc attttttcac
    32221 ctcaaatttt atgtatgtac aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag
    32281 ggggagatgg cgtgttactt tattttatca ttggttgctg tatcggatca tttttatgtt
    32341 taaccgccca gcggattccg ttaggtcact ctattattta tcctagatct cattgtgtca
    32401 aatgctgtcg ttcgctttct tggtatgaat tgattccgat tctctcgatc atcgtccaac
    32461 gatttcgctg ccgctattgc aggtgtcggt tgcccttcta ctatttgttg gcagaagcct
    32521 tatgtggtgg tctttttgcc tggttcttta ctttttcttc cgcaagaaat ttttcgacgt
    32581 tcatatggat gctttcggct ttgctatttt cgttgatgga tttattttac ttcatggtcc
    32641 attctcacac gctattctgt tcttgggcaa ttttatggat tttttggttg caggccggcg
    32701 tcttccaatg gcaaagtgtg ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg
    32761 gtcaatccta tctcggagct ggcgatactc tgttgatgct ttcttggagt ggtggcttgt
    32821 ctctagaaga gttgcttcag atcttgtttc ttgctagtct gtttggaata tccgtttttt
    32881 ctgtttactt tatctgtcat aaaaaaaagt tggaaaagct gcctttcatt ccttttttga
    32941 gcagtgcgtt gctgattgtc cttcatctta ggtattgaaa aaaggttgtg acaaacgact
    33001 tgtcacaacc tctgatatat agtgttctct taccaaatcc ttgtgacttg gagctaggcg
    33061 acaggttcac aacctctcca ctaaatggtg ttcaatcagc tgaccctcag tattagctca
    33121 aaatgctggt tttacaacct ctgatatacg gtgttccata agtagctttt tcggcttctt
    33181 atttcttgac gccccaatgt cgacgaagga aacctgcacg tccagaacct tctcgataaa
    33241 gattgtaatg gagcttgttt ttcttgtaat aatcctgatg gtaatcttct gcaggataaa
    33301 attcgcttct tggttcgatt tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca
    33361 atgcttgttt tgaacgctct gcaatatttc gttcttcttc atcattatag tagatcactg
    33421 gacgataaga atcaccgcga tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct
    33481 gccaatagat ttccaccaac tgatcataag aaataacttc tggatcatat tcgatctcta
    33541 ctgcttctgt atgccctgtt gtgcctgttg ttacttgttc ataagttgga tttggtacat
    33601 gcccgccagt ataaccggat gtcacagaaa tgatccctgg ctgctcttca aatggtttga
    33661 ccatacacca aaagcatccg cctgcgaaaa ctgctttttt tgtcatataa aatccccctt
    33721 ttttctcatc ataccttgat ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa
    33781 cggaaactat tatatacgtg atacggcttc tgataaatgc caaaacgaac gtacttgatc
    33841 aaaaacggtt aaatcaggaa tcacatattc agcagaagga agatttatgt tttcacctat
    33901 tcccaataca tgcatgttac cagccaaagc tgcttgacaa ccagcttctg aatcttcaat
    33961 gaccaagcat gcatttggcg gtacattcaa ttgctgtgct cctttgagaa aaacttctgg
    34021 atcaggtttt gcttttgata cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa
    34081 attcgttttt tctaaaatga ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat
    34141 gtttgttttt ttcaagtact caagaacagc tattgcacca ggcaaaacgg atgtttcatc
    34201 cattttttga atcgcttgaa gatatagatt gtttttttgc tcagcaaagg ctgatttctc
    34261 tttttctgag aatttctgtt ccatttttcc aaatgacagc aatcgctcta gtgactcggt
    34321 tctggatatt cctttcagtt gctcgttttc tttctcagtg aatggaatgg acagctggtt
    34381 agctaaaacc agccacgctt cgtaatgata acgagctgtg tctacaagaa cgccatctaa
    34441 atcgaatagt gctgctttga ttttcatcga ttacctcctt gaaaatgaac gacttgaagt
    34501 aaagatgcga tgtttttagc tgtgcgatga agattatcag tggcttgctg taaagcttct
    34561 tgtaaaggca cagctgcagg aaggatcgga aaaacggccg aaatgccaac atctgctgct
    34621 ttttcgcttc ctttaccaac acttccgcaa atagctatca caggaacgcc ctctggtact
    34681 cttctagcaa tcccaactgg cgcttttcct tttagacttt gtccatctag acgtccttcg
    34741 cctacgatta cgaggtctgc ttgctgtacc cgtttatcga actccagtaa atccaatacg
    34801 tagtcgatgc cagagactat tttccctgaa gcaaatgcta acagtcctgc acccattcca
    34861 ccaccagcac tactgcccga tgcctcgatt acttgagggg caaatctcat ataaaaatca
    34921 tgcatggctt ggtcagccac tgctaattca ttttctttca agcctttttg tggtccgaaa
    34981 acaaaagaag cgccttcttt tccgcataaa agattagtaa catcagtaat gacagtgatt
    35041 ttggctttaa tcagctcaat aggcacgttc gttttatcaa ctgtagcaat tttacctaga
    35101 ttttctccca gtgcttctac tttttgatta tcttgatcgt aaaaagcata acctagacct
    35161 tctgccatgc cgatgccgcc atcgttggta ctacttccgc caacgccgat gatgatgtgt
    35221 cgaacacctt gttgcaacaa ttgtcgtatc agttctccta ctcctcgcgt ggataaccta
    35281 agtggctgtt gttggtttag tgggacatct ttcaatccga cgatttctgc catttcaaaa
    35341 atcgcagttt gaccgtctga agcgtactgg atttctttgt ttttgccaaa tgcattagta
    35401 acgactgctg tttgccgctt caaccttaaa ctgtctgcta atgatgaaac cgttccttct
    35461 cctccatcgc ctataggtag cagatcatat tcagcctgaa caaaaacttc ttgaaaacct
    35521 gattgaatgg cttgtgctgc ctctaaggct gtcagacttt ctttgaaaga atctggcgca
    35581 atcagaatac gcattatttt cccctccgtt ccaagatcag ttctttttcc agctggtatt
    35641 ggtctccatt gacaaaaata ggtaatgttt ctttcgtctc tttttttatt actatttgtt
    35701 tttttgtcaa aacaaatagc aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt
    35761 cccatgctga aggaagtctt ggagaaatag aaagagttcc attcgttact gtaatacctg
    35821 aaaaaccaaa tataactgct agccatagtg ctcctaatga agcagcatga attccgtcat
    35881 ctgatgaatg cggatctggt cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg
    35941 cccattccat ttcttggata cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg
    36001 agtcgtggat tgttttgttt tcatagtaat gcaaattctt agaaacgatt ggttgagaaa
    36061 aatactcagg taataagtat aacagcatga caagatctgc ttgcttgctg acttggagtt
    36121 catttacttc gctacgggca taatcaagta aaatagactg gctgccagga cgttctttgt
    36181 aagtggtcaa atcaataact ggcttactga gatacgtgtc atcttgcgga atcagctgtt
    36241 ctttgttagg cactggcaaa tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa
    36301 gcggatcgcc agcttgatat ttaacagcta aggccacatt ataatgcgcc agatagttcg
    36361 tatatgcatt attgtcgata tattctgtgt attcatctgg accaatgact ctgtgtatct
    36421 ctaaatgatc atcgactttt acagctctgc tcagccaaaa agctgctgtt tcatgaatga
    36481 gcgtttttcc ctttgtttct aaaaacgatt gatcctctgt tgcttggaca tactcttgaa
    36541 cggcataagc aaggtctgca ctgatatgat gttctgcggc agctgaagca acttcttgtc
    36601 ttgttccggt tttgatattc aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg
    36661 cgctttccca tgggaaaagt gcaccttgat agtgataatc tcttgctttt tgccgtgcgc
    36721 cttctaatcg atcataacgg tacagcagca aattctttgc tacttcagga aactgatata
    36781 aaaagaaagg caggataaaa atttccgtat cccagaaaac atgcccttta taaccttcgc
    36841 cagttagtcc cttcgcccct acactaacac ttggatcatt gcggggagtc ataatctgta
    36901 aatggtaagc agcgaagtcc atcgctaact gatcgaaaga agaatcgcct tggatcgtta
    36961 tccgatgctg atgccaaaaa tctgtccaat aagacatcga ctgctctaaa atttctgtat
    37021 aactaacgct tttctgggct tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag
    37081 aggtagagag cacgatgatc ttctccagcg taaaggacgt attttttctg atcttttctg
    37141 aaaaggaagc ggtgatccgg cgattttttg cttggaatac acctgcttta ttgaatttag
    37201 ctgcaatccc aatatcaatt ttactttctg ttgtctggta ggatgcactg atcaaatcgt
    37261 caccaaatac tcgcagttct ttttcaacca actgctgtgt accaaaattg gtttgctgtc
    37321 catcgatacc cgttttcaca tctacttggc agtcttcggt caacggcttg atgaccactt
    37381 tcagggctgc ctgatggaga ttattttgat cagcaacacg acaagtaatg acttccactt
    37441 tttttccttg agcagtttca atgagcgcat agcgtttaag ctctcccatt tctaaatcta
    37501 gtgcccgctc gtatgcaaga actttcatcg tctcaagaga aaaatgttca ccgtcgattt
    37561 ttatttccca tctgatgaca tctgggagat tcactaactc ggctgattct gctcctaaag
    37621 gtcggttata tacaccagct gcaaacattc cccttacttg attccgatag tcttcctcct
    37681 ggcttcctct gataccaaaa aatccatttc caatggtcat ctgactcgca tacttattga
    37741 tatactctaa atcaaactgt ttttcttcta ctttcataag gtaataactt ctcctgtttc
    37801 tgccgaacga tataaatttt ccaccagatt ctggatgaca tatccttgcc agccatctgc
    37861 aatcatctga gtctggtcac cgagcgcccg attcacaaaa tgagccatac ttagttcatg
    37921 tgggtcatta actggcgtgg tcttttgtaa ggaaaccagc tttccatcta catcttcgaa
    37981 tagttcgcct ggatacagat tagcacctgc ctgatcgcca caaaaagcca cattcagttt
    38041 gtcttctctg atattcaaag caaacgaagt attcagttgg atgatcttgc cgttttctaa
    38101 ttccagtgtg ccaaaaattg catcttctac tgtgaatttg cttggatccc atttcccgaa
    38161 tttccctgaa ttttttttcg taccaatttt ttggaagctg tacgcccgaa cgctttttac
    38221 tttaggaaat ccagtaatat acatcgctgt atcaagcatg tgaatgccat aatcgatcaa
    38281 aggcccgcct ccttgaagtt ctttgtcgat aaacgttccc cagcctggaa caccagaccg
    38341 acgaagcgca ttcgcttcta cgtaataaat atccccgaga acatcaagat gattcttgat
    38401 cattgttgct tcgtcagaaa aacgatgctg gaatccataa gccaaaatct gcccgttttt
    38461 atcggctgtc tctgccatct ttcttgcttg atcagctgtc atagctggtg gtttctcgca
    38521 catcactgaa gcattagctt cgagggcagc caatacatgt tcgaagtgaa agcgattggg
    38581 tgtacagaca ctcacgatat cgggagaaat ctcattcagc atttgtgtaa ctgatgtata
    38641 cgcatgagaa aatcctgtac gtcggcaaaa gtcttctgct cgctggctga ctggatcaac
    38701 cacagcggcc aattctaaga catctgatct tttttgataa taagcagcat gtactttttc
    38761 tgctacttgt ccagcgccta tgattgctac tttcttcatt tatttctccc tcctttgctt
    38821 atttacttat tgttattctt tctctattct ttctctaaat catttaaatg acgcgctgtt
    38881 tccagacagt ttttcaagta ttgcaatgat tcctgataaa ttttcacttc attttctcct
    38941 cgcaaacgac attcatatac catataacct tcatactcat tttgaagtag cgtttgaaaa
    39001 tggcgagtaa aatcaattgc gccagatcct ggttggtagc gatgattatc tgctaaatgg
    39061 atatgaccca catactcttt ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata
    39121 ttcatatgat aaaaatcagc gatgatctgc acatgtttca attgattaga gtcaatgtaa
    39181 tgacgcgcat ccgctaacaa attgatcata tggtcttggt atcggttcaa aggctccaaa
    39241 taaataaccg tacctgtttc ttcggctact ttatctaaat aacgaagtga ttcgctgact
    39301 gctttatagt ctccttcttt gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac
    39361 attccccaag ctgctgggat aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa
    39421 atctcagcaa tttgacgtac accattaaga cgccgttctt cgataaagtc accgatccac
    39481 ccatcgtagc cgttacaagc tgtcactact gggatccctg tttctttgat tgcctgtttg
    39541 actgtgtcga tattttctat taacaactta ccgtcgattt cgtattcttc aaatccacag
    39601 tctttgataa atctaaattt ttctaaaatg tctcttggga aaaatgcttg atcttgcgta
    39661 ccgaatttca ttgttctcct cctcggactt gaacgcccat tttgatactt tcttctgggt
    39721 gctggtctac ataacgcatg taagcttctg cgctttctgc aaatggcaca actggatcaa
    39781 tgatttcttc acaatccaag taaccgttca tcaataattc ccaacatgtg tcctcgattc
    39841 gtttacggtt ccatcttggg taatctgggt ttggttcact agatgctcga gcaaagacga
    39901 tttttgcatt attgaaatgt gcttcgcgac caagattaaa cccagctgga aaaggtttag
    39961 caaaggcaac ataagaaatg atcccgccat agccgatccc tcttaaagaa gcttgtaatg
    40021 cggcagcact gccactcgtt tcaataactg aatctactcc acgtttatct gtcaattcct
    40081 ttagacgaaa accgacatct gctttcatag gatctagcgt gtaatcggca ccgtattttt
    40141 ctgctaattc tctacgctgc gctatgggat cgatcccaac gatggtagta gcacctgctt
    40201 ttttagctaa ttgaatcgca atttgcccga tagcgcctaa accgaagatt gctacagcat
    40261 cacctgctcg aacatgtgct tcccggatac cacctaaagc gaattgagca ggatcgtaac
    40321 atactgcatt tttccattga tccaaccgat caagtttacg tagtttataa ttatcaacac
    40381 cgtttgtaat cagcgtttca cgaatgggtc cataagtgca gactaactct cctttttgat
    40441 actctgtcac caaactgccc gtttcttcga tcgtaccgac aatcatattt cctaattgaa
    40501 attcgccaaa taccacaccg cgttggcttc cttcttctct tggcaagaat aggcgccatt
    40561 cttctgaaaa ttcttcatcg ataaatggac ttaatcctct aaagtcgaca atttctgttc
    40621 ctggttttgg agtagcgaaa attactcgga tacgtacttc gttcttttcg atctcacggt
    40681 cttcgtaatc tttgaatccg gctattcttg gttctaatgc aactaatttt ttcatctttt
    40741 ctttcctctt tcttttcttt ttatcctttt acgccgcctt cgactaaatc acctttaata
    40801 aatcgttcag ataacgcata cataatgatg actggtaaag cggtcaccaa tgaagcggcc
    40861 atcattcgtc cccaaatgta gtctggtgta ctgaataatg tgttcaaacc gattggtaaa
    40921 gtaaattttt cggaggtcga caggaaaatc gaagcaaata aataatcatt ccacgaaacc
    40981 ataaagcaat aaacgaagac agaaatgatt ccagaaatag ccaatggtat aatgatccga
    41041 ataatgatct ggatccggct aagaccatcg atcattgctg cttcttcaat atcagtcgga
    41101 atcgtgtcaa aataactttt tagcatgaat atcgcagtag gaagtgtttg aacgaccatc
    41161 gtgataatca gtgctgtatt ggtatcatac aaacctagac cggaaatgat tttaaataat
    41221 ggaacaacga tcaaaatacc ggaaaacata taaacggtgt aaaagctagc attgattttc
    41281 gagcgagctt tgaagcgtag ttttgacaaa gcgtatgccc cgctgatccc aatcactact
    41341 gcagttacgg aggctatcaa ggagatcact aaactatttt tgaaatacat gatgaatgga
    41401 aaaatctcag cattgaaaat atcctgataa tgttcaaaag tccattcttt cggaaaaata
    41461 gtttgtgtcg ttgcaattgc ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa
    41521 aatggaaaca aattgacagc taatagcaac aaaatcgtcg cgtaaaataa gattttcctg
    41581 ctctttttat ttttcatttc aagtccacct tctttcgtga gagcaagacg accgctgcaa
    41641 taatgatgaa caatagaatc gatatagctg ctgcttttcc aagatcattg aacgcaaagg
    41701 ctgtctgata caaataaacg cctaagatat tgactttatt cgtcagtaga tagacatctg
    41761 taaacatata gaacatccag atagatcgta aaacaatcac agttgctagt actggtttga
    41821 ttgccggcag tgtcacgatc ttgaacttct gccaagcatt tgcaccgtcc atttcagctg
    41881 cttcataaag cgttttgtcg actgtttgca aaatagccaa aaatgaaata aacgcatacg
    41941 gatagtatct ccagatagca aacaaaacga ccataaagaa gctagcaact ggctggtcga
    42001 accacagcgg tgcttcgttg aaaagctgca acctatctac taacagatca ttgacgattc
    42061 cataactatt attgaacata tatttccaag caaaaatcaa agaaatagat ggcgtaacat
    42121 aacttaataa aatcagtgaa cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa
    42181 tagctacggc taatcctaaa gctgtactgc ctgctacaac taagaatgtg taagcaactg
    42241 ttaaacctaa agaacgataa aattcactat cacttaaaat ttcaagataa ttgcttaatc
    42301 cgataaaaac ggcgtctgag ttaggattca gcggcatatc gaaaaaactg atctgaatat
    42361 tggaaagcat tggaatcagc actaaaacag ctaataaaag catgcttggt gctaaaagtc
    42421 ccatggctaa ttttaagtca gaatgtttcc ttggacgatt cattgaaacc tccccctgat
    42481 aatctgccaa tagctatcta cttttttcga acaccattgg cagaacttct tgttatttcg
    42541 tatttttttg tgcgtcagct aaagcttgat cgacatcttc gtttccgaca gttacttggt
    42601 tcactgcttt tccgatcaca cctgagcttg tgatatctcc cattttcgtg aaattctttt
    42661 ccccgactag tccaaagact tgtacttcat caaaagcact agcaatctca gatggcagct
    42721 cgccaaaaga cttgatgact tcattttctt tgtaaccatc cagttcgact acttgtttat
    42781 tcactggttg agcaccacca ggagacatca agacccactt ttccatgttt ttcggttgag
    42841 ataagaaagc tgtgaatgct tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa
    42901 tcgtcaatcc agatactgtg ccatagacag cttgatcttt ttgtgttggg atagcaaaac
    42961 cgatattttc tgagttcccc gcttcataaa cagaaggcaa aatgtaagtt gaatagattg
    43021 ccattggtgc tgttccattc atgaaagcat ctttgatttc tgtcacatca ttagagcctg
    43081 gcatcgtgta ttgggataag ttttgataaa acgacaatgc ttctctcatc ttttccgtat
    43141 cgatcgttac ctcgccttca tcatccaaaa catttgctcc atttgacaaa gcaaattgag
    43201 agaacgcttg ctcactcatt gttccttcag cagtaggcat agcaattcca tattgtttat
    43261 tctctttatc tgtgaaatgt tgagcaattt ttaaaatatc atcccaattt tctggttctg
    43321 aaaaaccagc ttctgataat ttttctttgt cataccaaat accttgaacc cagccgctaa
    43381 ttggtgcagc aatatagctt tttccatctt ctgaacgaac aaggtttttg gctccgtcat
    43441 aataattatc ttctccagct tcctcgataa cgttttggac tgccgtctga tcaatcagtt
    43501 catctttatc catcacttta gcaaaatcct gactgacttc cattacttct ggtaatttac
    43561 cggaacgagc taacgtaacg atttttgtat tgaacgctga ttcttctacc ggcacttgct
    43621 tgattttgat gtctggattt tctttttcaa aatcagcaac cagttcattg atcacactta
    43681 atcgttcctg ttcgacagaa gagtgcatga attgaatcgt tgtctgttgt gattcgttag
    43741 agctttgact ttcaccacaa gcagtaagaa gaaatccagt agctagcaga caggtagtaa
    43801 caataatttt tttcattttt ttccacctct tattttatta ttttcagcca catatattca
    43861 tacggctgga atatcaactt tcgttcttga ctgcaatgga taatttgtcc agaaaacagg
    43921 tctaggtaat ctccttttgg taaaagataa cctgctgatt gattggaaaa attatggata
    43981 actaagactt tctcttcctt cgcttcgcga ataaaactga taaaagaatc tgctgagtcc
    44041 agtaagtaaa tcaaactatt cggatggaag gctttttctt tttttcgaat agatagcatc
    44101 tgggtcaatt caccatatac tgctgttctt aatggatctg tctgcaactc tttctcaata
    44161 tctgctaaca aaaatttttg ccgattgatt tttcgattct ctcctgtttt ttctacgcct
    44221 ttgtaatcat ttcgacttcc cagcacactt tgaatataaa ttgccggcac gcctggtaac
    44281 gttaccaaaa atgaatgagc agctaaaaag cgtttgcttc tccattccgt cgtatctgtt
    44341 ggagaactta acgcatctag atacgtagcg tttatctcat atggactggt cgttccatct
    44401 gggtttttct tgtagtttac tcgggcaccc tctttcttca gctgttccac taattcaagg
    44461 atcgtttttt ccggcagaat acctcgtacc ggattcaatc cgataccgtc atgggaggct
    44521 aaaaaattaa aaaaagttgt ttttttcgat gcgaaatcga tggtttttaa ccattctttc
    44581 aacttacttg tattacccga acgaatcgct tgaagggtca aaggtggtag cgaaaactga
    44641 tagaccatct gtgcttcatt ctcgccattc ccaaaataac tgatattatc tttgtgtggc
    44701 acatttgtct cagtgatcaa taccgttcct ggagctgctt catcagcgat tgcacggaat
    44761 aattggatga ttttatgcgt ttctggcagg tgtatacaag aagttcctag ttctttccat
    44821 aaaaatccca cagcgtccaa gcgaataaat gaagcgcctt tttctaagta aaacaaaaga
    44881 atatcgatca ttttcaataa aacttctgga tttttaaaat tcaagtctac ttgatcttcg
    44941 ctaaaagtcg tccatacgtg tttttctttc ccgtttttgt caatgaacgt tgtaagtaac
    45001 gggctggtcc ttggtctagt cacgctgctt gtatctgtat ccagtggcaa atcaataaaa
    45061 aaatctttgt acgtatcatt caagtttaaa tattcctgaa accaagtact ttcggcagac
    45121 atatgattgc agacaaagtc gaacattaac cgtgtttcgc ttctcatttc ttctatatct
    45181 tcccatgtgc ccaaacggtc gttgattgcc tgataattta caacggagaa accatcatca
    45241 gatgtatatg ggaaaaatgg caagaaatgt acaactctaa atgtatctgc caaatacttt
    45301 tgataaaatt tcttaaatgt acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca
    45361 taagtaatca agaaaatatc ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc
    45421 tgctgataaa cactgacttt ttgttcaatc atagaggcgg ttttctcaga caaatctgag
    45481 tcgcgataaa tcgcttgtaa aagttttgta tacatcggat tcccctttct tttttgggag
    45541 cgttcccaac gatttgatca tattatggaa aacgcttact gtcaatagca tttttttaaa
    45601 taattttgat atacacaaaa atacaaataa gaaagcttat gtatcaataa ttaatagctt
    45661 cttaaaaaaa tatggtctct catgatcatg caagctattc ctaaattttc aaaatcatag
    45721 acaaaaaaga atgtctattg tactatggaa ttattctaaa acgctcccaa aaaggaggtg
    45781 catataaaat tgaaacctac tatttatgac attgcgaaaa aagctaatgt ctcaaaatca
    45841 actgtctcca gagtattaaa taaccagcca aatatctctg ataaagcaaa agcaagtgta
    45901 cttcaagcaa tcaaagaatt gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga
    45961 aatggttttg acgcgattct agcattatct catcgtactt ctcatacgac ctatggcaat
    46021 cctttttttt cagatgttat tcaatccatt tctgccgttt ctgaacaaaa taacttcgac
    46081 ttgattctac aaacggcaaa aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc
    46141 caagaaaaaa tgatccgagg aattattatt ttaagctcta cgatggatga aacacttctt
    46201 tttgaattgg atcaatacaa tattcctatt gtggtaatcg gaaaagtcgg aaacgactac
    46261 acgaacatct actcagtgga cacggataat ttccaggata gttacgattt ggtggaaaca
    46321 ctgatcaaga atggacaccg tcatatcggc tgtctccatg ctccgacaga agtttatgta
    46381 tccaatgatc gtctcaatgg ttaccgtcaa tgcttatttg atcaccacct agatatccgt
    46441 aatgaatgga ttatagacgg cggctttcgc ttagaagata gcatggcagc aatgaatgat
    46501 ttaatgaagc tacgagaaag accaactgcg attttttcta ccgatgcatt aaaatcattg
    46561 agtatttaca aagtggcaga acaatctgga aagcagatac ctgatgatct ttcgatcgca
    46621 ggattcagtg atcccttatt gtcctcccta ttttcgccac agttgactcg gatagacatt
    46681 ccgacaaatc agttaggaat caaagccaca gaacttttat tccaactaat ccaccaccaa
    46741 taccctgaag aaaaaagaat cattctttca accgagatta ctctcacaga ttcgatccaa
    46801 caacaagaaa tgtagaacag aattataaaa gaacagaaac actgaggata agtcagttac
    46861 tctgttcttt tttcaatcac tcatccctcg ttaccccctc ccggtatttt agcgaatttt
    46921 tcttattttc catctgtttt agatagctgt ggataactat aatgtttgtg ttaaactatt
    46981 ttttccacag ggaagaaaaa actattttta accacgttgt gtataagttt ttatagttcg
    47041 tttttttaaa cacatttacc cttatttcaa tccatgttat ttggggatac ttatctctaa
    47101 aacataaata gaataacaga catgtctact ggttttgttt tattcgagga attattcaat
    47161 aatagctagc aaaatattaa aaataaccgc tctatcttcc catcaattta ttttgcattc
    47221 aaaaaatgat ctatctttac ttttgttgat tgacttctct tggaagatga tcgttccgat
    47281 accttaattg gatacgaacc gatgttgtta agatcctttc ccacttttct tctagttaga
    47341 caaattattt tagacaaacc aaaaaagagg ttgtgacaat aatttgtcac aacctctttt
    47401 ttaaaatatc tattttgttt gattgttcat tgcgtcttct gcagccattt gcgcttcaat
    47461 atctgaaata ctgtagacat tttcgcttgc tacaccgact tcttttggtt ccatgtcacg
    47521 ataacgttgc ataccagtac cagctgggat gatcttacct ataataacat tttctttcaa
    47581 accaagaaga ggatctttct taccgcggat cgcagcatcc gtcaagacac gagttgtttc
    47641 ttggaatgat gcagcagata agaaactatt tgtttctaat gatgcttttg tgatacctag
    47701 taagacaggt cgagaagttg caggaacgcc tcctgagact agcgtattgt agttttgatc
    47761 tttaaagtct gcgatgtcta ataatgtacc tggtaagata tctgtatcac ctggatccat
    47821 tacacgaact ttacgcagca tttgacgaac cattacttcg atatgcttgt cgccgatttc
    47881 taccccttgc atacggtaaa cacgttgtac ttcacgaagc aagtagtttt caacagataa
    47941 tacatcgcga acttgcaaca attgttttgg atcgattgat ccttctgtca atggtgcacc
    48001 acggtggatc atatcgcctt ctgctacttt catacgtgct gtataaggaa cagtatacgt
    48061 acgtgtatct gtttttcctt tgattgttac ttctttggta cgtgttgcag gatcttctga
    48121 gatatcgatg acatcccctg ttacttctgt gatcactgct tgccctttcg gattacgtgc
    48181 ttcaaagatt tcttggacac gaggaagacc ttgagtgata tcgtctccgg caaccccacc
    48241 tgtatggaat gtacgcatcg tcaactgagt accaggttca ccgattgatt gagcggcgat
    48301 cgtaccaact gcttctccga cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca
    48361 atgtttgcag acaccatgtt ttgtattaca tgtgaagaca gaacggattg tcactgtttc
    48421 gacacctgca tcaacgattt gttttgcgat gtcttcggaa atcaattggt ttcctgcaat
    48481 gatcaattca tttgtttcag gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg
    48541 atcttctaat gattcgatga tttcatttcc ctcgcggatg gcttggattt caagacctcg
    48601 gtcagtaccg caatcttctt cacggatgat tacatcttgg gccacgtcaa ccagacgacg
    48661 agtcaagtaa cctgaatcgg cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc
    48721 gtgagttgag ataaacattt ccaagacgga tagtccttca cggaagttcg aaatgattgg
    48781 caattccatg attcgaccat taggagcggc catcaaacca cgcattccgg caagctgtgt
    48841 aaagttggaa atgttaccac gagctccaga atcagacatc atgaagattg gatttttcgc
    48901 ttccaaacct tccatcagtt tttgttggat gctgtctttt gcagcattcc aaacagcaat
    48961 gacacgttca taacgttcgt catctgtgat cagaccacga cggaattgtt ttgtaatagt
    49021 ttcaacttgt ttatgggcat tttcaatgat ttcttgtttt tcattcagta cactgatgtc
    49081 agcgatccct actgtgatac cggcatgtgt cgaatgtttg taccctaagt ctttcatacg
    49141 gtcaagcatc ttagaagttt cttttacttt gaatcgtttg aagacttctg cgatgatgtt
    49201 tcctaagttt ttcttcttga acggtaatac taattcttgc tctttgatat gtgcaggaat
    49261 atcagtacca gcttcaacaa agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt
    49321 tggttcattc agataaggga attcaggagg catgatctcg ttgaagatga tcttaccaac
    49381 tgttgtgatc atcgtacgtt ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt
    49441 agggttcaca ccgatacgtg aatgtaaatg aacatatccg ttgcgccatg cgataacggc
    49501 ttcgttttga tctctgaaga ccattccttc gccttcgcgt ccttcttctt ccattgtcaa
    49561 gtagtagtta cctagaacca tatcttgaga aggagtaaca actggtttac catctttagg
    49621 gttcaagatg ttttgagcag ctaacatcaa catacgtgct tctgcttgtg cttcttcatt
    49681 caatggaacg tggacagcca tttggtctcc atcgaaatcg gcattataag cttcacatac
    49741 aagtggatgc aaacggattg cgcgtccttg tactaaaact ggttcgaatg cttggatacc
    49801 aagtcggtga agcgtaggtg cccggttcag caatactggg tgttccttga ttacgtcttc
    49861 taaaacatcc catacttcgt cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt
    49921 tgaagctagt tcgcgttgaa caagctcacg catcacgaat ggtttgaata attcaatggc
    49981 catttctttt ggaagtccac attggtacat cttcaagaaa ggtccaacaa cgataacgga
    50041 acgacctgag tagtcgacac gtttacctag caagttttga cggaaacgac cttgtttccc
    50101 tttcaacata tgagaaagag atttcaatgg gcggttacct ggtccagtaa ctggacgtcc
    50161 gcgacgaccg ttatcgatca atgcatctac tgcttcttgc agcatacgtt tttcattttg
    50221 aacgatgatt ccaggtgcat tcaagtctaa caaacgtttc aaacggttat tacggttgat
    50281 tacacgacgg taaagatcat tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac
    50341 cattgggcgt aaatctggtg gaataacagg gataacgtcc ataaccatcc agcttggttg
    50401 atttcctgaa gcgcggaatg cttctaaaat atccaaacgg cggattgcac gtgtacgttt
    50461 ttgtccttgt gcagatttca attcttcttt caattcggct gcttctgcat ctagatcaac
    50521 attatcaagt aattgtttaa tcgcttctgc acccatagca gcttggaatt cttgtccata
    50581 ttgttcacgt ttttcgcgat attcgcgttc tgtcaacaat tgttttttct ctaaattcgt
    50641 atttcctggt tcgatgacta catatgaagc aaaataaatg atttcttcca aagcgcgtgg
    50701 gctcatgtct aatacaagac ccatacgtga aggaatccct ttaaagtacc agatatgtga
    50761 aacaggagct gctaattcaa tatgtcccat gcgttcacgg cgaactttcg aacgagtaac
    50821 ttccactccg catcgatcac aaacgattcc tttataacgg atacgtttgt attttccgca
    50881 ggcacattcc cagtcttttg taggaccaaa aatccgctca cagaacaacc cttcgcgttc
    50941 aggttttaag gtacgatagt taatcgtctc aggttttttt acttcaccgt aagaccagct
    51001 tctgattttt tcgggagaag ccaaacctat ttgcatactt tcgaatttat ttacatcgat
    51061 caaaaggggt tccctccatt tcatttaatg gcaaaccgca agacggctga tcttcgaccg
    51121 ccttgcagct catagaccgg attagtctaa attatcttct gcggtttcaa agttttggat
    51181 gacatcgtct ttttcatctt ccacttgttc agcagctttc ttttctaatt ctttggcagt
    51241 ttgctgttcg gcaaattttg tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc
    51301 catgtcgcga agttcgatct ctgtttcttc gatatccaag acacgcatgt ccagacctag
    51361 tgattgcaat tctttcacca atacgcggaa tgattccggt acacctggtt ttggaattgg
    51421 ttcacccttg acgattgctt cgtatgtttt cacacgtccg actacgtcat ctgatttgta
    51481 tgtcaagatt tcttgtaaag tataagccgc accgtaagct tccagtgccc atacttccat
    51541 ttcaccaaaa cgttgtccac caaattgagc tttacctccc aatggttgtt gtgtaaccaa
    51601 tgagtaaggt ccgattgaac gtgcatgcaa tttgtcatcg accatgtgag caagtttgat
    51661 catgtacatc acaccgacag acacacgtcc atcaaatggt tcacctgtac gtccgtcgta
    51721 cagaacagtt ttcgcatcgc tggccatacc ggcttcacga actgtttccc atacgtcttc
    51781 atcacttgca ccatcaaata ctggcgttgc tacgtggata cctaactgac gtgcagccat
    51841 acccaagtgt aattccaata cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt
    51901 cagcatgata tcgattggtg ttccatcagg taggaatggc atatcttctt ccggcataat
    51961 acgggataca acccctttat ttccgtgacg accggccatt ttatcccctt catggatttt
    52021 acgtttttga acgatataaa cacgaactaa catgtttacg cctggtgaca attcatctcc
    52081 agcttcacga gtaaagattt tcacatcgtg aacgataccg ccgccgccat gtggtacacg
    52141 taatgatgta tcgcggactt cacgagcttt ttcgccaaag atcgcatgca atagacgttc
    52201 ttctgcagat aattctgtta ccccttttgg cgtgactttc cctacaagca aatcgccatc
    52261 ttggacttct gcaccaatac ggatgatacc catttcgtct aaatctttta atgcgtcttc
    52321 cccgacatta gggatttcgc gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc
    52381 tgattcatat tcttcaatat ggatagaagt gtatacgtca tcttttacca aacgacggct
    52441 catgatgatc gcatcttcat agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt
    52501 ttggcctaga gccatttcgc cttgttccat agatggtccg tctgccagag tatccccttt
    52561 ttcgactttc tcacccaaat gaacgattgg acgttggttg tagcttgttc cggagtttga
    52621 acgacggaat tttgttacat cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat
    52681 ttcagaagca tcaacgtatt caacgacacc atcatgttta cataacaaag cagctcctga
    52741 gtcatgcgct gatttgtatt ccataccagt accaacccat ggtgcttgag gattgatcaa
    52801 cggcactgct tgacgctgca tgttggcacc catcaaggca cggttggagt catcgttttc
    52861 caagaaagga atacatgctg tcgcaacggc aactacttgt ttaggtgata cgtccatgta
    52921 gtctacttta tcaatagata cttctaagtt ttcactttga gcacgagcca taacaacgtc
    52981 ttgagcaaat gttccatctt cattcaatgg gctgtttgct tgggctacga tgtaatggtc
    53041 ttcgatatca gctgtcaagt agtcgatttg gtcagtaaca cgacctgttt cacggtctac
    53101 acgacgataa ggtgtttcga taaatccgaa tttatttact ttcgcataac tggacaaact
    53161 attgatcaac ccgatgtttg ggccttcagg agtttcgatc ggacacatac ggccatagtg
    53221 ggagtagtga acgtcacgaa cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc
    53281 taaggcgaaa agacgacgtt tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa
    53341 ctgtgacaac tgagaagaac caaagaattc tttgatactt gccacaactg gacggatatt
    53401 gatcaattgt tgaggtgtca atgtttcagt atcttggatc gacattcttt cacgaaccac
    53461 acgttccata cgtgctagcc cgatacggaa ttggttttgt aaaagttcac cgactgaacg
    53521 gatacgacga tttcctaagt ggtcgatatc atcgacattg ccgatacctt ccatcaagtt
    53581 caagaaatag ctcattgaag caatgatgtc ggctggacga accgttttta cagctgcttc
    53641 tggatagccg tttccgatca cgtttacttc acgttcaggg tctcttggtg aaaatacttt
    53701 gatgacttgg acagtcattg gatctgtgac cacaccgtct tctgatggat agtaagtcac
    53761 tgaatttagg ccattatcaa tgaaaggagc tagtgtttcc atgacttggt gagtcagaac
    53821 tgttcctttt tcaacgatga tctcgcctgt ttctggatca actaatgttt cagctaatgt
    53881 cagattcaat agacgtgttt tcaaatctaa ttttttgttt actttgtaac gaccaacgtt
    53941 tgctaagtcg taacgttttg gatcgaagaa acgagcattc agcaagttac gtgagctgtc
    54001 tgctgttttt ggttcgcctg gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt
    54061 tcttgagtcg cttgcgtttt tatgcaagtc tttttcgact gtattgcgta atgtttcgct
    54121 gtcgccgaag atttcaaaga tcgtatcatc ggaaccaaat cctaacgcac gaactaatac
    54181 ggttaaagga atcttacgtg tacggtcgat acgtacataa gagatgtctt ttgcatcagt
    54241 ttccatttct agccaagctc cacggttagg gataacagta gaaccgaatc cttcttttcc
    54301 gtttttgtcg acttttccat ggaaatagac acctggtgaa cggactaact gagaaacgat
    54361 tactcgctct gccccgttga tgatgaatgt tccttgttct gtcattaatg ggaaatcacc
    54421 gaagaatact tcttgtgctt tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac
    54481 atgtaatggt gcagagtagt ttgcatcatg cgcacgagct tctgctactg tatactttgg
    54541 ttctttcaat tcataatcta caaattccaa agatagattg ccattgaagt catcgattgg
    54601 caaaatgtct tcaaacattt ctctaagtcc ctcatctaag aaccattggt aagagtctgt
    54661 ttgaatttcg atcaaatttg gtaattccaa cacttcactg attcttgcga aacttctacg
    54721 ttcgcgatgt tttccgtatt ttactacgtg tccagccaag ctcttcaccc ctctaaaatg
    54781 tcattcaaaa agactacccg atcaattgtt acaataatat ttcaaatatt atttttaggt
    54841 aacaacagac attttttgcg gcttttaggc aaaaaaaaac caaaaataga acattctctt
    54901 gaaaccttac ttcgtgagct tctacttttg tttccttata ttaaagccga aacggacaat
    54961 atttcgtttc tgccttttct tcatcagata gtttttgcaa cttcatatac tacacttatt
    55021 ttctataaaa gtcaacactt cctggcgttt cttctgcttt ttcgcaacat tttcttaatg
    55081 ttttcaattt tatgttttca attatctgac aattcttaat tgacagacct ttttatctcg
    55141 gttatacttt agaaaattca ttttgtggag gaacttctat gcgaaccaac atcaaccaaa
    55201 tcgcaccaac acaactgaat aacttttact ttagctactt tagataggtt tgtattcatc
    55261 atgatggata caaacgttaa acgacagttt gtatctatga tggctaaaga actttgtatt
    55321 gcagcaaaaa gccacgtaga caaaatgcta agtggctgag cagtgaaaat acaaagtaaa
    55381 agtatcaagc attctacttt ttcaatttat tttccgactt ttcaatccat ttggacattt
    55441 tgtctgaatg gattttttgt ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat
    55501 aatcatcgga ttaggcgctg ccctgctact agctttagga ggttgcgcac aaaaagacaa
    55561 ttccgcagag gcatcagatg gacaaaaaac aattggcgta ttgcaacttg tacaacataa
    55621 ctcgttagat gctgcctata aaggattcaa agaaggatta gcagaagaag ggtataaaga
    55681 aggcgataat ctaacaatcg attatcaaaa tgcccaaaac aatcaagaca acctgaaaag
    55741 tatgagtgag aaactcgtca aagaagatcc tgatttgcta cttggaatcg ccactccagc
    55801 agcacaatct ttattaaatg aaacaaccac tatcccgatg actgtcacag cggtcacaga
    55861 cttggaacaa gcccatttag tcacatccaa tgaaaaacct ggcggcaatg tgactggcac
    55921 gacagatatg gtgccaatcg aaaaacaaat cggcttactg ttaaatatcg tgccagacgc
    55981 caaaacaatc ggcatcatgt ataacaacgg tgaagcaaac tctaagatcc aagctgattt
    56041 ggcagaaaaa gaattgaaaa aagcgggcgt caaagtcaaa gtactgacag caaattccac
    56101 aaatgatgtc caacaagtga cgaaaagtct agctaaagat gtagatggaa tctatattcc
    56161 tactgataac acgttcgcaa gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa
    56221 aacacctgtc gttgctggcg cagttgatca agtcaaagac ggcggtctag caacagtagg
    56281 aatcgactac gaaagcttgg gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg
    56341 caagaatcca agtgaacttc ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa
    56401 agacatggcc aaagcattag gcatcaatcc agacagcatc aaagaagctg aataaaacac
    56461 aggaggattc tcatgacaaa tttgctctct cttttcttat catctgcatc gcaaggggtc
    56521 ttatgggccc tacttgcgat tggtgtatac gtcactttcc gtatcttgga cattgccgac
    56581 ttgactgctg aaggaagctt tcctttaggt gcagggatcg cggcagtaag tattacaaat
    56641 ggctatcatc cattgacagc ttgtcttcta ggctttttag ggagtgcggc agcagggctt
    56701 gtttccggac tgcttcacac aaaattgaaa attcccgccc ttcttgcagg gatcatcacc
    56761 atgactggtt tatattctgt cacatcccgt gtgatgggtg cgccgaacat ctctttattg
    56821 ggtcagccaa cgatctttca ttgggtcgag tcattcggca taacaaaaga caacgctgta
    56881 ctgatcgtcg gtctgctttt tgctttgatc gttgtatgtt tacttgtttt gttcttacgt
    56941 acagaaacag gacttgctat tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac
    57001 gggatcaaca cagataatat gaagatcatc ggttatatga tttctaatgg atgcattgct
    57061 ctttctggct ccttgcttgc gcaaaacaac ggctttgctg atttaaattc tggtattgga
    57121 acgatcgtaa ttggacttgc ttctattatc atcgcagaag ttttgttacg taaccagcca
    57181 ctatttctcc gtctcttgac tgtcgtttta ggtgcggtca tttatcgttt tattttagcg
    57241 ttagtcttcg aactgaatgt tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta
    57301 gtgatctgtc tttctttccc gcagatccag caaaaattcc atttacctca aaaaaagaaa
    57361 ggcggtagtg tcaaatgaat gacgtcttaa cattggaaaa tctccatcat acctttgaaa
    57421 aaggaacgat caacgaaaat catgtcctga aaggcattaa tctttcctta gatcccggcg
    57481 acttcgtgac gatcatcggt ggaaatgggg ctggtaaatc tactttgctg aacagcatcg
    57541 ccggtacatt ttctgtcgat cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa
    57601 aatctgttgt cgaacgttcg aaatccatca gtcgcgtatt ccaagatcct aaactaggga
    57661 cagccgttcg tttaacggta gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac
    57721 gcggcttctt ccgtggtgtc aaaccacaag accgaacatt tttcaaagac cagctggttc
    57781 ggctaaattt ggggttggaa aatcgtttat cctcagaaat cggtttgctg tctggaggac
    57841 aacgtcaagc tattacgtta ttgatggcca cattacagcg tccagaactg atcttattag
    57901 atgaacacac tgcagcgctt gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa
    57961 agctgatcac tgagcagaat ctcactgctt ttatggtcac tcatgacatg gaagacgcga
    58021 tccgctatgg gagtcgactg atcatgctgc atcaaggaag gatcgtcgta gatatccagc
    58081 aagaggaaaa acagcagctc acagtgcccc aattattagc gttgttcaaa caaaacagcg
    58141 gcacggcatt aaaagacgat caagtacttt tatcttcata atcagaaaga ggatgggaca
    58201 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
    58261 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
    58321 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
    58381 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
    58441 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
    58501 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
    58561 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
    58621 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
    58681 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
    58741 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
    58801 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
    58861 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
    58921 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
    58981 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
    59041 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
    59101 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
    59161 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
    59221 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
    59281 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
    59341 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
    59401 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
    59461 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
    59521 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
    59581 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
    59641 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
    59701 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
    59761 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
    59821 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
    59881 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
    59941 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
    60001 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
    60061 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
    60121 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
    60181 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
    60241 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
    60301 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
    60361 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
    60421 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
    60481 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
    60541 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
    60601 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
    60661 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
    60721 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
    60781 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
    60841 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
    60901 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
    60961 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttc
//
LOCUS       DANJMA010000011        59101 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000011 DANJMA010000000
VERSION     DANJMA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(60..188)
                     /locus_tag="PWU31_000845"
     CDS             complement(60..188)
                     /locus_tag="PWU31_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1083410.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="PWU31_000846"
     CDS             complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="PWU31_000846"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1083411.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1675..2730)
                     /locus_tag="PWU31_000847"
     CDS             complement(1675..2730)
                     /locus_tag="PWU31_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1083412.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="PWU31_000848"
     CDS             complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="PWU31_000848"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL1083413.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="PWU31_000849"
     CDS             complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="PWU31_000849"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL1083414.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="PWU31_000850"
     CDS             complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="PWU31_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL1083415.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="PWU31_000851"
     CDS             complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="PWU31_000851"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL1083416.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="PWU31_000852"
     CDS             complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="PWU31_000852"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL1083417.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10492..12051
                     /locus_tag="PWU31_000853"
     CDS             10492..12051
                     /locus_tag="PWU31_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL1083418.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12162..14093)
                     /locus_tag="PWU31_000854"
     CDS             complement(12162..14093)
                     /locus_tag="PWU31_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1083419.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="PWU31_000855"
     CDS             complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="PWU31_000855"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL1083420.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15022..15774)
                     /locus_tag="PWU31_000856"
     CDS             complement(15022..15774)
                     /locus_tag="PWU31_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1083421.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15933..16142)
                     /locus_tag="PWU31_000857"
     CDS             complement(15933..16142)
                     /locus_tag="PWU31_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083422.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16153..17481)
                     /locus_tag="PWU31_000858"
     CDS             complement(16153..17481)
                     /locus_tag="PWU31_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083423.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17617..17772
                     /locus_tag="PWU31_000859"
     CDS             17617..17772
                     /locus_tag="PWU31_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083424.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17846..18433)
                     /locus_tag="PWU31_000860"
     CDS             complement(17846..18433)
                     /locus_tag="PWU31_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL1083425.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="PWU31_000861"
     CDS             complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="PWU31_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL1083426.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20013..21302)
                     /locus_tag="PWU31_000862"
     CDS             complement(20013..21302)
                     /locus_tag="PWU31_000862"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL1083427.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21456..22388)
                     /locus_tag="PWU31_000863"
     CDS             complement(21456..22388)
                     /locus_tag="PWU31_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083428.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22545..23204)
                     /locus_tag="PWU31_000864"
     CDS             complement(22545..23204)
                     /locus_tag="PWU31_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1083429.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="PWU31_000865"
     CDS             complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="PWU31_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL1083430.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24245..24535)
                     /locus_tag="PWU31_000866"
     CDS             complement(24245..24535)
                     /locus_tag="PWU31_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083431.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24945..26750
                     /gene="glmS"
                     /locus_tag="PWU31_000867"
     CDS             24945..26750
                     /gene="glmS"
                     /locus_tag="PWU31_000867"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL1083432.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26822..28177)
                     /locus_tag="PWU31_000868"
     CDS             complement(26822..28177)
                     /locus_tag="PWU31_000868"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL1083433.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28201..29370)
                     /locus_tag="PWU31_000869"
     CDS             complement(28201..29370)
                     /locus_tag="PWU31_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083434.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29367..30254)
                     /locus_tag="PWU31_000870"
     CDS             complement(29367..30254)
                     /locus_tag="PWU31_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL1083435.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30443..34123)
                     /gene="nifJ"
                     /locus_tag="PWU31_000871"
     CDS             complement(30443..34123)
                     /gene="nifJ"
                     /locus_tag="PWU31_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL1083436.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34321..34842
                     /locus_tag="PWU31_000872"
     CDS             34321..34842
                     /locus_tag="PWU31_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1083437.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35541..36106)
                     /locus_tag="PWU31_000873"
                     /pseudo
     CDS             complement(35541..36106)
                     /locus_tag="PWU31_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36285..36941)
                     /locus_tag="PWU31_000874"
     CDS             complement(36285..36941)
                     /locus_tag="PWU31_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083438.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37509..38444)
                     /gene="whiA"
                     /locus_tag="PWU31_000875"
     CDS             complement(37509..38444)
                     /gene="whiA"
                     /locus_tag="PWU31_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL1083439.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38478..39476)
                     /locus_tag="PWU31_000876"
     CDS             complement(38478..39476)
                     /locus_tag="PWU31_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL1083440.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39473..40357)
                     /gene="rapZ"
                     /locus_tag="PWU31_000877"
     CDS             complement(39473..40357)
                     /gene="rapZ"
                     /locus_tag="PWU31_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL1083441.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40492..41223)
                     /locus_tag="PWU31_000878"
     CDS             complement(40492..41223)
                     /locus_tag="PWU31_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL1083442.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41227..42492)
                     /locus_tag="PWU31_000879"
     CDS             complement(41227..42492)
                     /locus_tag="PWU31_000879"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL1083443.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42755..45571)
                     /gene="uvrA"
                     /locus_tag="PWU31_000880"
     CDS             complement(42755..45571)
                     /gene="uvrA"
                     /locus_tag="PWU31_000880"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL1083444.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNNRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45581..47575)
                     /gene="uvrB"
                     /locus_tag="PWU31_000881"
     CDS             complement(45581..47575)
                     /gene="uvrB"
                     /locus_tag="PWU31_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL1083445.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47834..49336
                     /locus_tag="PWU31_000882"
     CDS             47834..49336
                     /locus_tag="PWU31_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL1083446.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49338..50075
                     /locus_tag="PWU31_000883"
     CDS             49338..50075
                     /locus_tag="PWU31_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1083447.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50443..51609)
                     /gene="dnaJ"
                     /locus_tag="PWU31_000884"
     CDS             complement(50443..51609)
                     /gene="dnaJ"
                     /locus_tag="PWU31_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL1083448.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51760..53589)
                     /gene="dnaK"
                     /locus_tag="PWU31_000885"
     CDS             complement(51760..53589)
                     /gene="dnaK"
                     /locus_tag="PWU31_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL1083449.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53640..54203)
                     /gene="grpE"
                     /locus_tag="PWU31_000886"
     CDS             complement(53640..54203)
                     /gene="grpE"
                     /locus_tag="PWU31_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL1083450.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54297..55340)
                     /gene="hrcA"
                     /locus_tag="PWU31_000887"
     CDS             complement(54297..55340)
                     /gene="hrcA"
                     /locus_tag="PWU31_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL1083451.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55575..56741)
                     /locus_tag="PWU31_000888"
     CDS             complement(55575..56741)
                     /locus_tag="PWU31_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL1083452.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56757..56984)
                     /locus_tag="PWU31_000889"
     CDS             complement(56757..56984)
                     /locus_tag="PWU31_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL1083453.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57371..57627)
                     /locus_tag="PWU31_000890"
                     /pseudo
     CDS             complement(57371..57627)
                     /locus_tag="PWU31_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57970..58993)
                     /locus_tag="PWU31_000891"
                     /pseudo
     CDS             complement(57970..58993)
                     /locus_tag="PWU31_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16291 a        12807 c        10278 g        19725 t
ORIGIN      
        1 ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct ttagcacgtc
       61 tatttattaa agattttagg gccttttctc atgtttttac gatcattctt aggtgtttcc
      121 atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat tttttctttt
      181 tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga tgggtttgcg
      241 ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca aataacttag
      301 catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct ggtgacattt
      361 cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca actacgatca
      421 cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct tttccgactt
      481 cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct tcaattgctt
      541 ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac aagccaatcg
      601 tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg gcaaactcga
      661 tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat gactttgctg
      721 agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca tataaaactt
      781 tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg atgaccgaaa
      841 ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta aagaattttt
      901 ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga cggataatga
      961 tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg aaccgttgca
     1021 agatatctct gactactttg attgcttgat cgatgacagg atcttggata taaaaatctt
     1081 cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac tgtccatcga
     1141 tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc attccgaccg
     1201 ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc tctacttgtt
     1261 tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg atacctatgc
     1321 gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata aatgtttcga
     1381 cttttccata tccaaactta ttgcctgctt ttactactag tatttctact tcttctccag
     1441 ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc attccttcat
     1501 aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt tgctgttctg
     1561 tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta taaatcagct
     1621 aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt ttgattaatt
     1681 cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt catccgttaa
     1741 acgttcgact tcttttacga attctttgct gacaacctct aattcttctt catctgtacc
     1801 tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt gttgatgaag
     1861 attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga tcattactgg
     1921 tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat agattattct
     1981 tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca agatctcaaa
     2041 gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt tggcatctgg
     2101 cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca tcgaagcatt
     2161 tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta ctcgagggag
     2221 catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta gttctgatgg
     2281 aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga agtaactgtt
     2341 atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt ctgctgctgc
     2401 tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg taccagctgg
     2461 gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca cttcgttgat
     2521 tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga ctctacatgc
     2581 ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt caaatcctgc
     2641 ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt ttcccgaggt
     2701 ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg ctaattcatc
     2761 tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt tcatgatttg
     2821 accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga cagattgttg
     2881 gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag ataattgcac
     2941 ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca attcacggaa
     3001 gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa tcattcctgc
     3061 taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt tcaagtaagc
     3121 agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat tttccaaaac
     3181 agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt catactctgg
     3241 aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag gcaaactagc
     3301 ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag gctctgggaa
     3361 gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat tcgttgtttc
     3421 gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt ttgcttgacg
     3481 tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt ttttcaattc
     3541 tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg catcacagcg
     3601 catagagcct tcttccattt tcacatcgct gacttctgta aattggataa ttgatcgcaa
     3661 tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag aaacgatttc
     3721 gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa taccatgcat
     3781 gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt tctttttacc
     3841 ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt gggaaatttg
     3901 atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag tatctttaga
     3961 gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag ctttattcat
     4021 tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt ttggttctgc
     4081 accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact cgacgtggac
     4141 ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat agctggttgt
     4201 tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat cgttttttca
     4261 tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa acctgccggt
     4321 acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc actcatgtac
     4381 attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg tgaagatggc
     4441 ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat caatgtacgg
     4501 acttgtcccg cttttttgaa gtaagcgtca taataaccag cacttaagga gaatgtacct
     4561 aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt gacatataca
     4621 tcatctaatg atttcacatt ttctgagcga taaccgtagc ggataccatc gaatcgttgc
     4681 aagttggaac ttgcttctga tgaggcaatg atataataaa cagccacacc gtattttgaa
     4741 tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc tgctgctttc
     4801 aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt aggtaaagca
     4861 atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac tgaaacgcct
     4921 gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa tgcattatct
     4981 ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc aatcaaacca
     5041 aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa agctgctggt
     5101 tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc agctgcaact
     5161 gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca agcatttttt
     5221 gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc catgtttagc
     5281 ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc atcataaatt
     5341 ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt tgttacgata
     5401 ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat ccctttttca
     5461 tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa tgcttcgacc
     5521 tcaggctctg tttctttgat tcgttcaaat gttgcttttg tcaaatcttg tacagtgatt
     5581 tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa ttctgtcatt
     5641 atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg tcttctgttt
     5701 ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga tcttgtcgca
     5761 tcacgtttac tgtttcaacc acattcgacg taaatggaac accttctgta tctacttctt
     5821 ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt gcaagttctt
     5881 catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct tcactaatcg
     5941 ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt aacacaagag
     6001 tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt cattcttcta
     6061 ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact tcgattccta
     6121 attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg atatccgttt
     6181 ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct tttgcttctt
     6241 cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta aaaggagact
     6301 cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg cctgcttgtt
     6361 ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact acactgtcgg
     6421 caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt ttactcaatg
     6481 tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg acatgtcgga
     6541 ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg attgctgtca
     6601 aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct tcttctgtca
     6661 atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt tctagtacgc
     6721 gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc aaaccttctt
     6781 tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca tccaaatgga
     6841 caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg ctgtctttag
     6901 gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct ttataaatca
     6961 gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta tgcaggctgg
     7021 cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt ggcgttacta
     7081 cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact tcttctggcg
     7141 gaaacttgta agctgctgcc catctcggtg ctttgacagt aaaaccaagt tgatcttgta
     7201 gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa tccacacgtt
     7261 tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat agacgttttt
     7321 cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac tgggtttttg
     7381 cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt cttttcgctg
     7441 tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga ttcgcaaaga
     7501 tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt tttggcatga
     7561 aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg accgaccgga
     7621 cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga gtcgcacctc
     7681 tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt tttagttcac
     7741 agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa gctatcagct
     7801 cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg actttttcaa
     7861 aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg atcaaatcag
     7921 gatattgttc ctcaattgct actaattctg cgtattcttt atcataaaca tagtcttcca
     7981 ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt gtacgaagtt
     8041 cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt taccctccac
     8101 tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc ttgacttggg
     8161 aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac tgttcctaga
     8221 ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc ccctccagta
     8281 gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc tgttttttta
     8341 gcgaatacag gtgttttttt aggcgtagga cgcatgcctt cgatttccag taactcctga
     8401 tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata taatgttctg
     8461 gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac atatgcgaga
     8521 cggcgttctt cttccagttc gctttcttcc atcaatgctc gagataaagg aaaaaccccc
     8581 tcttctagac caatcaggaa tacaactggg aattctaatc ctttggctgc atgcaaagtc
     8641 atcaatgtca cttgggaagc atcttcttct aaattgtcaa tatcggaaac aagtgccaga
     8701 tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc attttgctgt
     8761 tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt ttcgatgcgt
     8821 gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct acgatccaaa
     8881 acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac ttcttggatc
     8941 atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc cagatccaca
     9001 ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg tagtttttcg
     9061 atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa actgattgaa
     9121 tcttgaggat ttgccagcac attcaaatac gctaaaatat ctttgatttc tttacgatcg
     9181 tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag agtttcttcc
     9241 attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta attccgatga
     9301 ttgcttcgaa tctcttcttg catccgatct acgataaaac gggtttcgtc tcgttcatta
     9361 tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa gttttttggt
     9421 tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt tttcgtggag
     9481 cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc aaaatccaag
     9541 atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc tcccacgacg
     9601 cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc gtgatttgta
     9661 tcttggtact catctacatg aatataatgg aatttgtttt gataatatgt gagtgaatca
     9721 ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc catacattga
     9781 ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc ttcgtaataa
     9841 ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc ttgagagatt
     9901 gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc ttttagtatc
     9961 cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg gttatacccg
    10021 ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt cgatacccag
    10081 acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc ttttgcagcc
    10141 ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc ctcaatcaaa
    10201 taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc cattagcagt
    10261 aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc gtttaataat
    10321 tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa ttatagcatt
    10381 ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta tggatttttc
    10441 cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg aatggaaaaa
    10501 aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa aggaatattt
    10561 gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga aaagctaagc
    10621 gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt aattggccta
    10681 gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa tgaaccggat
    10741 atcaagggaa gcgggattcc acaagtagag ggacagcttc gcggggaatt atcaatgaac
    10801 tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg tgcgggcctt
    10861 tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca aggagctgga
    10921 caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg tgcaagtagc
    10981 ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt agaagaaatc
    11041 catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat cactgctaac
    11101 ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga ccttccttct
    11161 ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg catcttaggt
    11221 tttatttatc agaaggtctt gctggctttg ccaaaatttt acaaccgatt gcctttacca
    11281 gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta tttatggcct
    11341 agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc cttgccaatc
    11401 attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc ttacggtggt
    11461 ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat cggtagcttt
    11521 tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga ttttgtcgtt
    11581 attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc aattatttta
    11641 gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc tgctcttgtt
    11701 gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt attggaacgt
    11761 ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt tgattttcca
    11821 gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg gcctaaaaat
    11881 atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg tgatacgatc
    11941 atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc aagaatcaag
    12001 caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta acgaacatgg
    12061 aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga ttctataaaa
    12121 aaagaagaga ctgggaaaaa ccagcctctt cttttttatt tctatgcttc tgctgctgca
    12181 ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat agcaataaac
    12241 accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc atgaggagcc
    12301 agtaatttga tcccgaaagc tccgactaat ccgccagtta aagcagaacc aataataaaa
    12361 cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc cgtgacaaac
    12421 gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct gaatttattc
    12481 ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc agccataaca
    12541 gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc tgtaccaaac
    12601 acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc agctaataat
    12661 gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt caatgcatca
    12721 ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat cagtaagcca
    12781 aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa gttcttgaat
    12841 aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag tgcacctaag
    12901 aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat caaaccaggg
    12961 cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa accaaatgca
    13021 gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag atttgataat
    13081 tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat agcgattcct
    13141 ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt gtaaattctt
    13201 tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc atcaccatgg
    13261 aatactggtg cagtaccact aagggctaga ttgatcagct cttctggttt acggatacca
    13321 tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc tacttttttg
    13381 tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa tcggtttttg
    13441 attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt cgcttttttc
    13501 ttcagagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc tgtaacggca
    13561 acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc ttgttctgct
    13621 ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac ttcttctggt
    13681 gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg agatagtgct
    13741 gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat gaaaaacaga
    13801 taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt tgcgaataaa
    13861 actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc atcaccaaga
    13921 cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc gatatcggaa
    13981 atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt tttatcagtc
    14041 gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt catgattatc
    14101 ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca tcaatcaatt
    14161 cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact ccccatttaa
    14221 aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccggca atcattgaat
    14281 cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta taagttcctt
    14341 gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc tgtgcccctt
    14401 cagctagcaa ttttttacca tacggaatga tttcttctac agattggaag gttgtattgt
    14461 aaagctcagc taattcgtga ttgtttggtt tgatcaacaa tggtttctta acaagtgcat
    14521 tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct cgaatgatat
    14581 caatcagttc ttgataaaat ccttttcgca ggctagccgg cgcgcttcct gacaatacga
    14641 cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt tcttcatctg
    14701 aaatagatgg gcccaaaccg ttaatttcag tttcagtatc tgattttagt ttgatattga
    14761 ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt tcaagccact
    14821 tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat tgagaatcga
    14881 tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg aagtcttcct
    14941 tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa tcaattgatg
    15001 gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct tttcacggaa
    15061 ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt ccaaaggtgc
    15121 aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa atgtctcttc
    15181 ggaatttttc atggctaaac gtttaacagc tgcctcttct gggtccggag tcgtgaatcc
    15241 ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa aattttttag
    15301 ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg ttccacctaa
    15361 aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg aatttgtcac
    15421 tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag agccagcgtc
    15481 taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga tttgctgttt
    15541 ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca ataattgctc
    15601 tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct cttgtaaatc
    15661 acgtcttatc gttgattcag aagcattaaa aaggtttaca agatcttgtg atttcacgat
    15721 tttctgctgt tcaagcaagt ctaggatttt ttgtctacgt tcctctgtaa gcattttcac
    15781 gatcacctca atacaaataa tagcatattt tttttctact cgcaatataa atcagtcaaa
    15841 aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca aaaagactgg
    15901 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    15961 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    16021 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    16081 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    16141 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    16201 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    16261 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    16321 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    16381 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    16441 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgcg agcgattctc
    16501 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    16561 atctccttgt tgaatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    16621 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    16681 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    16741 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    16801 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    16861 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    16921 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    16981 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    17041 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    17101 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    17161 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    17221 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    17281 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    17341 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    17401 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    17461 taatttatct tcgccaatca ttttctcacc tctttaaaat ctttttataa tttaattata
    17521 accaacgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    17581 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    17641 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    17701 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    17761 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    17821 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    17881 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    17941 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    18001 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    18061 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    18121 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    18181 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    18241 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    18301 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    18361 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    18421 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    18481 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    18541 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    18601 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    18661 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    18721 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    18781 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    18841 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    18901 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    18961 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    19021 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    19081 tgtttacgtc ctccctgagg tggcacgctt gcaacagttc cttctagaat tttcagcaag
    19141 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    19201 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcacgttc cacattgtaa
    19261 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    19321 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    19381 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    19441 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    19501 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attcaatact
    19561 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    19621 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    19681 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    19741 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    19801 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    19861 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    19921 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    19981 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    20041 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    20101 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    20161 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    20221 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    20281 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    20341 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    20401 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    20461 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    20521 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    20581 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    20641 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    20701 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    20761 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    20821 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    20881 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    20941 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    21001 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    21061 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    21121 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    21181 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    21241 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    21301 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    21361 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    21421 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    21481 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    21541 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    21601 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcatt taacacggaa
    21661 ctaagcagcg catcttcatt accgtaacga tctgaaaata cacataattg ctggtaactt
    21721 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    21781 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    21841 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    21901 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    21961 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    22021 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    22081 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    22141 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    22201 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    22261 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    22321 tttgctagtg cttgttgtgc tactcgcatt aaattctcat atttttctgg aaaatcaatc
    22381 ggcttcacgc agtgtcctcc tcttgctcat gttcagctca tgttcaatag tagcaaaaca
    22441 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg
    22501 acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc
    22561 tggttgaatg ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgcct
    22621 agatgttgct ttttctgatt aaagattgct ggaagatacc ctaacatcag caaaggaaat
    22681 tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct
    22741 tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg accatgattg
    22801 aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagata
    22861 ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa
    22921 cgcgtaatag ctccgataca caaaagatcg accaagaaag caaacattcg gataaaaaaa
    22981 cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta
    23041 tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca
    23101 aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc
    23161 ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt
    23221 aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct
    23281 gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac
    23341 tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt
    23401 cctttcgcat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt
    23461 cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag
    23521 atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg
    23581 cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca
    23641 acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat
    23701 tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt
    23761 tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat
    23821 acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt
    23881 cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt
    23941 cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata
    24001 aacctgaaga ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg
    24061 tactgtctcc ctcttccaac acatttgaag ctagttcttc atccccatat aaccaactgt
    24121 tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa
    24181 ccacaaataa tcctgcagca atcagaactg ctgcccaacg tctcttattc attctttctc
    24241 ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa gtgtcgaacc
    24301 gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgtcaat tcatcttcta
    24361 ccgtaaactc acgagcttca gcaacttcca aggcatcagg tgcgatttct tggatgctat
    24421 cccagccagt atctaaaaat attttcgtat catctgatac accagctgat gcttcgaatt
    24481 tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa tccattttta
    24541 ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat gatagataaa
    24601 aacccattat tttttgacat aaaaagagca tttgacaaag catttgatat ataccaattt
    24661 attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt ttaaatgttt
    24721 ccagataaaa aacaaaagag gaggatagtt atacctttta ggatagcgcc aggtctgtgt
    24781 aaacagatga cgaggagagg gtttatcgaa agattcggcg gatggcccta gggactgcac
    24841 tctacagaaa atccacaaaa aatatctgca aagataaaac aaaaggattt tcctgcagct
    24901 ggaagcattc atacgaattc attatagaaa gtaggatgtt ttttatgtgc ggaattgttg
    24961 gaatgattgg attaaaaaat gtaacaccag gattgattga cggtctagaa aaattagaat
    25021 accgtggtta tgattcagct ggtatctttg tctcagatgg cacaactgat tatctggtca
    25081 aagcacaagg cagaattcaa aatttgaaaa acaaaataaa tattgatact acaggaaata
    25141 taggtattgg tcatacacgc tgggcaactc acgggcaacc ttctgaagaa aacgcccatc
    25201 cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa tggcgtaatc gaaaactttg
    25261 aagaacttaa aattgcttat ttagcaaatg accactttat tggtgagacc gatactgaga
    25321 ttatcgctca tctaattgaa actttcgcga aggatactac aacgcaagag gcgtttttaa
    25381 aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt gattgatcgg acagcacctg
    25441 atgttattta cgtcgctaaa aataaaagtc cactattaat cggactcggc gatggattca
    25501 atgtgattgc cagcgatgca atggctatgc ttgctcatac gaaggaattc gttgagattg
    25561 aagatgaaga gatggttacc gtcacatcag aaaaagttat tattcaaaat tttgcaggtg
    25621 acatcgtaga acgttcttca ttcgaagcac aagtagattc ttcagatatc gagaaaggta
    25681 cctatccttt ttatatgctg aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg
    25741 ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga tcaaaagctg gtcgatacat
    25801 tgtctgatag cgatcgaatc tatatcgttg cttgcggaac tagttaccat gcggggttag
    25861 caggtaaaca gactctggaa aaattgacac agatccctgt tgaggttcat ttagccagtg
    25921 aatttggcta taacacacct cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa
    25981 gtggcgaaac agcagatagc cgccaagttc tggtaaaaat caaccgtctc ggctaccctt
    26041 cacttacgat tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc tttacactgc
    26101 tattgcatgc tggacccgaa atcgcagtag catcaacgaa agcttatact gcacaaatag
    26161 ctgtactaac tttattagcc aaagctatcg gtgataaaaa agaatttgaa acttctcttg
    26221 tattcgatat cactcatgaa ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc
    26281 aaaaagatta tttagaagag ttggcagaag tatatctcag cgatacaaga aatgcctttt
    26341 acattggacg tggcgctgac tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa
    26401 tcagttatat ccaggcagaa ggttttgccg ctggcgaatt gaaacatgga acgattgctt
    26461 tgatcgaaga aggaacacca gtgattggaa tcatcagtga agaagtgact ggtgcgcata
    26521 ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa aacactagtt attgtctcag
    26581 agggattaga aaaggaaagt gaccagctgg tgctaccaac tgtccaccct tatctaacta
    26641 ctctaaccac agtcgtaccg actcaattac ttgcatatta cgcaacgcta ttacggggat
    26701 tcgacgttga caaaccacgt aatttagcga aatcagtgac agtagaataa taaaagaaca
    26761 aggaccagtg tcagacatct gacactggtc cttgttcttt tattttttat gaaaatatct
    26821 cttaatccag tccgatttct tctttgacta ctgccgcgat tttatcgaca tagtaattaa
    26881 ctttctcatc agtaggcgct tctgccatca ctcgaagtaa tggttctgta ccggaaggac
    26941 gaactaggat tcttccttct gatcccattt cagcttctgc ctcttcaatc acttttttga
    27001 ttgccggaac atccatcgca ccgtttttat cacttactcg gatattcact aatttttgcg
    27061 gatagattgt tacctctgct gcaagctctg ataatttctt acctgtctgt ttcatgatat
    27121 tcagtaactg aattcctgaa agcatcccat ctccagttgt gttaaaatct aagaaaatca
    27181 tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt gcgcatttct tctacaacgt
    27241 aacgatcacc aacttgtgta acgacatctt tcaaaccgat tccttccacc gctttgtgga
    27301 atcctaagtt actcatgaca gtcgtaacga tcgtatcttt tttcaatcga tttttttctg
    27361 ccaagtatct agcacagata aacatgattt tatcaccgtc gacaatgttt ccaagttcat
    27421 ccaccgcaat aatacgatcg ccatcgccat cgaaagctag tccagcatct gctccttttt
    27481 caacgaccat ctcagctaaa cgttctggat gggtagagcc tacgccatca ttgatattta
    27541 acccgtctgg atttgtgccc atggtataaa aatctgtttc aagatcggca aacaaacgat
    27601 taacacttgt tgaagttgct ccgtttgctg catcgacaca aatggtcaaa ccggaaaggt
    27661 caccgttgat cgtttgtaca aggaattgag aatatttcaa taagccttct ggaaattctt
    27721 ctacagttcc caatccttca gctgatggac gaggcaattc atccacttca gcatctaata
    27781 atgcttcaat ttctagctct tggtcatcta ctagtttaaa accatctgca ccgaaaaatt
    27841 tgattccatt atcttgtgct ggattatgag aagcagaaat catgacccct gcgcttgctt
    27901 tttgcaaacg tgtcagataa gcaacacctg gtgtagagat cacacctagc tggaacactt
    27961 cgatacccac cgataaaagt cctgaaatca gcgcttcttc taacatttga cctgagattc
    28021 ttgtatcccg ccctactaac acgcgtgggc gttttccttc ttcttcatgc tggcttagga
    28081 cataacctcc gcaccgtccc agcttaaatg caagttctgg cgtaagttcc ttgttggctt
    28141 caccgcgtac gccgtctgtt ccaaaatatt tacccatagt tgtaaaatta ctcctttaac
    28201 ttaattttct gaggaatttg ttctgctcat ttgtgttgat tcgcttgttt ctgatgtttc
    28261 ttctgtactt gtggtcgagc tgcttgtccc agattccgaa ctggaagaaa tggttgttgt
    28321 cgatggttcc gttcctgaat cagcagatga aatacttcta ctcgtacttg tattttcttg
    28381 tgtccgactg ctcgtcgaag cagagctact gcttgacgtt tgcaacacgg gtgtaatctc
    28441 cacactaact gattcgggct tgatacttaa cccctcttct actggtattt caattttacg
    28501 agttgttcga tgattgatac ctgagacatt gatcggtaca gcaatactgt caccgatcga
    28561 tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt tttgaaaaac tgaaatcata
    28621 gctttctacc ccggcagctg gtgttccttg ctgtatccca taaagtcgta ccgacttgct
    28681 tggcttgatc gaatcagctc tcactgttac ttgcactgga tcagagacga tacttaatac
    28741 ttcccctgct gcattcagcg cctgtattgg gacctcacta ttaatcccat catctgtgac
    28801 catcgaagcg tccacatcag ctactactcg tttgatttct gccaatgttt tgtcacctgt
    28861 agtgatagag acttttttag gactaacgga agtatcatca agttcatatc cagatggtgt
    28921 gaacgttgaa ggaatcaacg tttccacatc gaaatctttt attacctttt tctcaaccgt
    28981 tactgtaata gtcgatggtt cgatctttgc tgtgacagca ctgctcaaat tttgaatttt
    29041 tagcggaacc tcatgtgttc cttcgcccag cttagtgata tctgctgtga cgcggaacat
    29101 ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg tttgcactgg aaagtttgac
    29161 agtcactgca ttttcatatc catggatata gtagcgcttt gaatcataag aaatcttgat
    29221 tggcacccca gtaactgttt gattatacgc ttcatttcca gaaagcgtat ttggaacgga
    29281 ctggccattt gcattgaagt acagcgcaat actgaacagc aatgcaataa aactgtaaag
    29341 aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc ctcccttagt cataccgtct
    29401 aggaaagatt gaatcacgtt tttcttgttt tttgttttct cttcagcaat cagttctcta
    29461 cgcaaaattg ccaaatattc ttgctgggat aagccagcca tcagttcatt atctaatgta
    29521 atgctgacat ccccagtttc ttcagagaca acgatggtaa tcgcgtcgct gacttcgctt
    29581 ataccgacag ctgcccggtg tcgagtcccg aattcttttg gaatcaacat actttctgat
    29641 agaggaagat aggcgcttgc tactgcgatc tttccttctt ttacaataac tgcaccgtca
    29701 tgcagcggtg tattaggaat aaagatattg atcagcagct ctccagtgat atctgcatct
    29761 aatgcaattc ctgtctcaat atactcatct aatccagtat gccgttcgat agtaattagc
    29821 gcaccgattt ttcgcttgga catatattgg atggctttgt caaaagacaa gatcatcttc
    29881 tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg aactgcgtcc aagatgttcc
    29941 agtcctctcc ggatttccgg ctgaaaaatc acgatagcag ctatcacccc gtacgtaatg
    30001 acttggttca tcagccaaga caaggtgtgc agaccaatga tttccgctac gattcggata
    30061 acaataaaaa tagctacacc tttcagaagc tgaatcgctt ttgtgcctcg cagcagttgg
    30121 atcagtttgt atacaagata ccagacaacc agaatatcaa gtatgttcac caaaaaattc
    30181 agagagaaca agtcaccgga aaacatttgc tgccaatagt ccaaattaaa aagttccccg
    30241 atttgaaatg acataaaaag cctctcttct cttttgagat ttcctctcta gtatatcata
    30301 tgttttaatt ttagaggctg atctgctcgt atttttcttg agaaaaacgc ataattttca
    30361 ggaaaattgc aagtatgcgt tttatttttc tctgcaataa atatttttaa acttcagaga
    30421 attattcatt atattttttc tgttattttt cctttttttc ttttgtagct tgttccattt
    30481 ttttacgtcg ttctgctctg gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt
    30541 cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt ttcttgattt
    30601 tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg tcttggatag
    30661 tcttttcaaa taaagctttt gcaagatttg gatttgcgtt ctctagcgag gaaaagcgaa
    30721 cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag tccaaagtca
    30781 ttgggttctt gtttttttcg accattgcag ggttatatcg atataatgac caatagccag
    30841 aagaaacagc ttcttttgct tctttaagcg attctttcat tccaccaagc aaaccatgtg
    30901 tgatacaagg cgtataggca atgattaaag aaggtccagg atacttttct gcttcttcaa
    30961 aagcctttat cgtctgcatt tgattggcac ctgaagcaat ttgcgccaca taaacatttc
    31021 catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca ctagccgaaa
    31081 atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta ttggaataga
    31141 cttcgttatc caaaacaaga atattcacgt ctgctccgct ggcaagtaca tgatcgatcc
    31201 cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg ctgtttttta
    31261 cgaatagatc ctttctcttg tatatatcct ccagttcagg gacaattgat cgttcgcttt
    31321 cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcgctctca aacatgtgta
    31381 ccaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat acttctttga
    31441 ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca taaccaaatt
    31501 cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat tcattggtcg
    31561 tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc gcattcgcga
    31621 tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt tcgccacagc
    31681 ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac aagacagtat
    31741 tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc caattcacag
    31801 cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt gcgggacaag
    31861 cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata cggtatttta
    31921 cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct tccatttcct
    31981 cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca cattcattac
    32041 acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc cgcttttcca
    32101 tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca agtgtaccaa
    32161 cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt atataaccag
    32221 tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct gggatcgcga
    32281 cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct acgatagcca
    32341 ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc acctcatcaa
    32401 aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc
    32461 ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta atgttatttt
    32521 cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg tcgctccaaa
    32581 gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca tagctatgca
    32641 ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac gctgaacgta
    32701 tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt ttcgaatcat
    32761 aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg atagctgatt
    32821 tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg aatgtttgcg
    32881 gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca tcatccacga
    32941 tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg
    33001 ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca ccaataacag
    33061 ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga ggttctccac
    33121 ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta tgaggcaact
    33181 tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa aaacctgttt
    33241 tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca actgatccca
    33301 tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag tcataagaag
    33361 ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt aatttatcat
    33421 aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg ttcgtaccac
    33481 taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat ttctcctgat
    33541 tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc tcgtgactcg
    33601 tacggaatcc atcaaagaaa ttcaaaaaag gtacttctcc ttcaatagaa gcaagatggg
    33661 caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata gcaaatcctg
    33721 tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca tttgtcgtca
    33781 ctgcccgact agcaacatgg aaaacacttg gcagtagttc cccagcaatc ttgtacatat
    33841 tcggtatcat caaaagcagc ccttgagaag cggtatatgt agttgtcagt gcaccagttt
    33901 tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct actactttta
    33961 ctggttgacc gaatacattt tcttttcctg ctgctgacca ttgatctact aattcagcca
    34021 tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca taagaaatat
    34081 atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat catccttgtt
    34141 catattctct cttattatct gttccattat aacgtaaaac attccatttt ttcttttatt
    34201 tataaaaata tttcacatat gtctatttga attggtcatc taatttttag acaaacagtc
    34261 taaatcgttg cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac
    34321 atggatatat cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt
    34381 tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaacc tatcttgtcc gtattgccga
    34441 caatggtttg aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag
    34501 cgagtgatca aattgttcga taaagaaaaa gaaagcttgc agcgaggaaa cgtgatgcac
    34561 cggtatctca caatcagcga tggtcaaaaa gcgatcaaag agatcaaaca aatattcgat
    34621 actcaagacg agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa
    34681 ctaaagctga cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag
    34741 caagcacata ttcgatttgt tccgactgtc atcttgggta aagaaatctt tgatgaatct
    34801 atttcaatag aggaattaaa agaattgatc caaacaaaat aaaaaaataa ggagcgtagg
    34861 acaaaagtaa agaacacttt tgtctcacgc tctaaacacc tataaacggc ggaagcagaa
    34921 gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcctt attccgaata
    34981 aatggtgtcc aaaagcttgc atctgccaga ttatctcatt tcttatgaca agctgagcaa
    35041 attatctcca ccaaataatt tattcatatt tttgacttgc gcatactaac taattcttaa
    35101 attttcatac aaaatttctc aaattccctt aacgagtaat atttatgtac ggtgattcac
    35161 actagtatcc caatcacttt ttaagttatc ccgatttcaa ttctacttca aaaatagttg
    35221 cttttatgta tgctgttagt tgattagatg gtcgtttgtt gagctaggtc gtttatttta
    35281 aacaagttgt ttctttagct tatacctatc tcgtctacca aacgcaagat ggcaaacaca
    35341 aatgcatacg catctgtatt tgccactttg attcgtataa agctaacaac tcctaactcc
    35401 tctcatttaa tagttgctct cttttttatt cagatacatt tttgctgatc acagcctagt
    35461 atttcaatgg ttttgaacac taaaccaaat tgttccatta caacattcac tggctgatga
    35521 aatggattaa tagatttcga ttattcaaag cgattttcta ttggtggatt gtgttcccaa
    35581 ctaacaagac ggattccaaa gtcattatat aactcttcaa gagttaaccc tttcctgttc
    35641 ccaatagatg tatctctccc acgctcgata taaaatatgg agtttcgaat gccaagacct
    35701 ggcaaatctt tccttgttac agttacactg ctactaactt ccccatataa aatccagctc
    35761 ctccaaaatg ataatatatt tttcgaccat catctaactc atgtaacaat aattgttgtg
    35821 ccctcttgtc actcgccaaa tgcaaacgcc acgtgcgata ccatgccatg ctacctgcat
    35881 ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat
    35941 ctacttcata ttctaatcta aaatcaaact cccctcgggt aatcgcaggt cttggtggct
    36001 taggacccaa tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa
    36061 caacaataat gattaatatt ttgtttctat ttgataaatt tctcattatt cgcaaatcac
    36121 ctctacattc tcacatacta gttcgtaata ctcctttaat atcaaaatat ctactgatcg
    36181 taataatatt cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt
    36241 acttgctgat gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg
    36301 ttaacaatcg gttcagatat ttcccaattc actagcctta tattatattg atttaacaat
    36361 tcttcttcat ttattgtatt ttgatcaagc ggaccaaatt gttctaataa ttctttgtca
    36421 tgtaaaatat cccttctagt aatgcctctt tccctttcaa tgaaagaagc actaggaaaa
    36481 gtatgattat aaccattcaa cctatctccc atgtaataat tagcaggtcc tggaaaataa
    36541 aatatttgct tattatcact aacttctaac agcaacacta aatcttctcc gctggctaaa
    36601 cgacttgtcc atctacgata tctccctatg ccttcgttca ttccaaatcc atcaaattca
    36661 actattaacg tgtcttctat cacatgtaat tcttcattca attcatactc aagtctaaaa
    36721 ggaaattctc catatgtaat ctctggcaat ggtggtgctg gagttctcca aataaataca
    36781 ataactgtga atagaaaaaa tccaacgatc gatataaatg ttatgagacc tgctcgaagt
    36841 aaaacccgat ataataaaaa tgctgtattt cgctttgcta tatctagaaa aataaaaagt
    36901 actgcaatca taatgatagt aaaagttgta aaaaaacgca tttttgccct ccctttataa
    36961 acatcgattc tcaaattcta aaccaaatct tggtagttca agttcaggta taacgtattc
    37021 caaccataca tctcaattac tatttatgaa cttatgatta tcactgaata aatcactcca
    37081 tccccttgtt aaaacttcta cattaattac acctggttgt tttcgactag ctaatccaat
    37141 gaacttatca accatcctta tccaccaacc acgagaatct tgaccagaag taagaagagc
    37201 agttctcaag gatgatcgag cagttccttc cagtttttta attaatcagg ccagttatag
    37261 ataaatactt gatcttatct atatcaggac ttctatatta agcaaaaatt tatgtacaaa
    37321 actaaaatct tcattcagtt gtctccccgc tttttatacg attgtattaa aaacaataga
    37381 agcctttcca aattatgtta ctccagtaaa gatggaatta tagtttgacg gaagaaaagc
    37441 aagtataact tttaaaaaaa actgatacat tcgcatctcc tgcttcatgt atcagttttt
    37501 tctttggatt aaactgcttc ttcacgcagt ttttccgcaa attcatttat tttgcgaatt
    37561 cggtgattaa ttcccgattt tgatatcgca cctgagggaa tcatttcccc taattctttc
    37621 aagctgacct ctggatgttg aagccgaagt tctgcgattt cctgaagttt ttcaggaagg
    37681 gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt cgatttgttt agaagccgca
    37741 tcaatcgtct tgttcagatt tgctgtctca caattgacta gacgattgac cgaatttctc
    37801 atatcccgta cgattcgaac atcttcaaat cggagcattg agttggttgc accaatcaat
    37861 gttaagaaat cagcaatttt ttctgctcct tttaaataag aaatatagcc attgcgccgt
    37921 tcaagcgttc gagcatgtag accgtaataa tttaacatat cgcaaatgtc ttggttatgc
    37981 tgttcataaa tagaaaatat ttccaaatga taacggctcg tttccggatt attgactgaa
    38041 cctgaggcca taaaagcacc acgtaagtaa gaacgcattt tttgcgaatt tcccataatc
    38101 tcgtccgaaa cgtgtccatt gaacatcata ccatccatga tatcaagatc catcaacaca
    38161 tcttttgtgc cttgcttcaa ccggacgata tagacattgt tcttttttaa tttcattttt
    38221 ttacgaacga gtagctcact tcgtacctga taatgatctt tcaaaagaga atagatacgt
    38281 ctggcaattg cagcattttc tgtttggacg ttcaaaataa actgctggtt cacaatacct
    38341 aaagagccgt tcattcgcag caaagcagct aattccgctt tcgcatgttc tcgatgaact
    38401 tccaaaccag ttagttcttt cttcacatct gcagcaaatg acatcctatt ctcctccttt
    38461 ctccaacaat aaattgctta atattttgaa ccgaaaacta aacggaacaa ttcttctact
    38521 actttgtctc catcgtggaa gacaccacca tctttcaatt ccaaaaagtc ggtggaaatc
    38581 actcgacatc cttcatttct caatccgttg aagtcgtgtg cgacttgtac tagatactca
    38641 tcatagatct ctgggtccat ataatcttca ggcacttttt ccgtattgac caatactgta
    38701 tcgataaaag gctgattcaa atgcttgtgt aaaacacgta tatgatcggc atctgtgaaa
    38761 tgttccgttt cccctttttg ggtcatgata ttacagatat agacggtttc agcagttgtc
    38821 ttgagcattg cttcgccgat ttctggtatc actaaatttg gaaggatact ggtgaataaa
    38881 cttccgggtc ccaaaacgac catgtcggcg tccataattg ctgaaacgac ttttcgagcc
    38941 gctttggctt cacttttatc atttgtgttg cagacataca cacggtcaat ggttttacgg
    39001 tcgatcgcga ttttagattc tcctactgca gtcgttccgt ctttaaagac agcatgcaag
    39061 gtcaaaggag tttctgaaga aggataaaca tgtccatcta catgcatcat ttttgctaat
    39121 aattgaatcg cctcataagt gcttccccgc atctcggaaa ctgcggcaat gatcagatta
    39181 ccgatcgtat gattggctaa aaactgatct tcctttttga aacggtattg gaaaatatct
    39241 tcatagaatt gtggcatatc cgataacgcc actaagacat ttctcaagtc accaggagga
    39301 gccatagcga tcgattctct gattgcaccg ctgcttcccc catcatctgc aacagtcact
    39361 actgctgtga tatctgctcc ttgatttcgc aaacttttca ggataactgg cagaccagtg
    39421 ccaccgccga ccactacgat ctttggtttt cttatccggt aggttttcat tttcacgacc
    39481 ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt cacattgact ttatagtttt
    39541 tccctaaagc atttgccaaa cgttcagtca atgccacaga acggtgttga ccgcctgtac
    39601 agccgattgc aactgtcaag ctgctttttc cttctttaat gtatccaggc aatacagttt
    39661 ccagaagatc caaaaattta tggtagaatg attgcgtttc atcaaaggac atcacataat
    39721 catagactgg ctgatctttt ccggtcaatg gtcttagttc cgggatataa tgagggttgg
    39781 gcaaaaaacg cacatccatc acaatatccg aatcgatcgg caacccgtat ttaaaaccaa
    39841 atgatacaaa ttcgatccga aatccttggt ctccctttga tttgaatgac tcattgattt
    39901 tttcacgtaa ttgtcgtggt gtcaatgtcg tcgtatcaat gatcacggaa gcttgggctt
    39961 tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc agtgatcaaa ccgtccatcg
    40021 ccattggatg tgctcgtctt gtttctttgt agcgagccac tagttcttcg tctgaagcat
    40081 ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc gatttcgatc agcatgtttt
    40141 ggatttcttc aaagaaggaa cgagaacgta aatccactcc taaagccatt tttgtgactt
    40201 tgcctgattc tttgattaat tcccagaatt ttggaatcaa tcgaggcggc atattatcga
    40261 tacaaaaata gcccatatct tcaaaacttt ggatagctac tgttttccct gcaccgctca
    40321 tacctgttat gatgactaat tgcaaattat ctgtcataat tacgaactcc tctcacattg
    40381 tgcatagtaa aattatatca ttccgggcgt atcattacca gttttctttt tcttttacaa
    40441 aaagatttgg accaaatatc ttttaggata tttgccccaa atccaatttt tttacttttc
    40501 cgatgctgta tccaatgcct tgcttctttc agctagcgct cgttcaatcg tccgatctgc
    40561 taaaatagat tgggcatcga tcactggata atggttatct tcagcaaatt catgcatcaa
    40621 tgacaattct gtgcaaccta agataacttt ttcacagttt aggcgctcta ccgcttcttc
    40681 taagatttcg taatatagct cttgattcaa ataatccgat tccttgatct catgatagat
    40741 cagataattg attttttctt gaagaacagt atcaggaatc actgtttcga atccaagatt
    40801 tttgacttcg cgttcataga ttccagcttt catacttcct tctgttccta gaatcgctac
    40861 tctccctgtc gtatgctgac ggacaagttc atttgctgct tctcttggca tatgaaggat
    40921 aggaatatcc gttgccgctt gtaattcttc aaaaaagtag tgtgcggtgt tacaagtcaa
    40981 cacaatgaag ttcggctgca ataaattttg tttctcaata tcatccagca gaaacggcat
    41041 tggattttct tctgatcgat ctaaaatata agctgtacga tcaggaactg ttgcatgatt
    41101 gaataagaca taattcaaat attcttgatc ttttgtagct ttcgttcggt ggttgatcaa
    41161 gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct aaaatactga aaaaattctc
    41221 catgaactag ccttctttca catgaaaata ctttttgaat cttggaatat agttatggaa
    41281 catatatttc atcaacaacc agcgcttgat tgaccggtct ttttcataaa acacagtagt
    41341 accgtaacga ttttctttga tcatttgctc agctagtttt ttgtcttcat tttcccgagc
    41401 atattctaaa aagatttttt tgggcacacc catccataat cttgctttat ctgattgatt
    41461 cgttccatag gtcgtttcaa caaagggttt gttaaacact cgatcttctg tgacgaaacg
    41521 agccagattc aagccgttca atgtcacaaa gaaactgcta cgaccttgtc tcaaattgat
    41581 ttcaaataat ttatattctc catctctagg atcatacttc atatcgaaat tggcaaagcc
    41641 tgtatattcg attttttcca aaaatgcctt aatggtttga tagatttttt cgttataatc
    41701 tggcatgatc acaacgtaat taccaatcga tgctggtgta ggatcttcta acagtggatg
    41761 accaagacac atcatccgaa cttgatgatc ttcatctaca tacgcattta gcacacgcat
    41821 attactatca tcacccggga taaaatcttg tacaatcatt tcacttttat aaccggcttc
    41881 gtatatccgg cctaaaatta aatcgaactc ttctctagtc tctaaaataa aagctttttt
    41941 tcttccttca aattggacag aaagatactc cacactgttt gcaggtttca aagcaactgg
    42001 gaattcaaaa ggcaattcct gttccaaatg tccatttact aacatttctt ctctgacgat
    42061 caatgtttta ggatacggaa gatcatattc ttcacaaact tcataaaagc ttactttatt
    42121 gatcagtctt tcaaataaag aataatcaat atatggacag ataaaatatt ctgacagctc
    42181 ttgtttatgt tgggagatca attctgcata tccatcacca caagcaatca aaagatattt
    42241 tttactctta tcagaatatc tttcttttgc caaacgaagc atcgtctcga taaacacagg
    42301 gtctttatca aatcctggaa taacttctac attgacaatt ttactgtagc gggtaggggc
    42361 taactgatcc gaagcatatg cttggcagat tttgccgtat gcttcattaa aagaacgtgc
    42421 cataccataa acgttcatgt cgcttcctaa taatataggg ataaattctt cattttcaat
    42481 actgttcatc attatctctc acttactcat aatctcttgt ttttcttttc caactacaca
    42541 aggaatatca taccataaag aaaaaagcaa tcaaaggaaa ctcctccgat tgctcaattc
    42601 ttttttgaac ggattgaact tataggaagt ttttgtctat accaatcagt gtagaaagaa
    42661 ctaccatgta atgattctag aacactgata ttaataaaac aagcaaaaat tcattttttt
    42721 atgttttttt aactatctca gctatctatc ctctttatac cattattttt ttcaaatagt
    42781 gacctgtata actttctttt acgttaatga tttcttctgg tgttccagtc gcaaggatcg
    42841 ttcctccacc ttctccacct tctggtccta aatcaatgat ataatctgct gttttgatta
    42901 catctagatt gtgttcaatc actaaaactg tgttaccagc atctactaat ctttgtaata
    42961 catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt cgttggttca tctagtatat
    43021 agaaattctt tccattagag attttgtgca attcactggc aagtttcatc cgttgtgcct
    43081 caccaccgga caatgtcgtt gctggttgcc ccatcgtcac ataacctaag ccaacatcaa
    43141 caatcgtttg cagtttgcga tgaatctttg gaatgtgctt gaagaattct acagcatctt
    43201 ctactgtcat ttccaaaata tcagcaatgc tttttccttt gtaatgcact tctaatgttt
    43261 cagagttata tcgtttgcca tgacatactt cacaaggaac atagacatca ggcaaaaagt
    43321 gcatttcgat cttaataatt ccatccccgc gacaagcttc acaacgaccg ccttttacgt
    43381 tgaaactaaa acgccctttc ttataacccc gcatttttgc ctcgttcgtt tgagcaaata
    43441 acccacggat atcatcaaat acacttgtat aagtcgctgg attacttctc ggcgtccggc
    43501 cgattgggct ttgatcgata tcgatgatct tttcgatact ttcgtagccg gaaattgtct
    43561 tgaatttacc tggcttagca gaattcttat ttaatttttg cgctaacgat ttctttaaga
    43621 tactattgac caatgtactc tttccagaac cagacacacc tgtgactgct acaaattctc
    43681 ctaatggaaa ttcaacacta atatttttca gattgttttc tgaagcacca gtgatcttga
    43741 ttgctttccc attaccttta cggcgttctt ttggtacagg gattgatttt ttccctgaaa
    43801 gatatttccc tgtcaaagaa ttttcatcat tagctacttc ttcaggagtg cctgcagcca
    43861 cgatttgtcc gccttgttct ccggccccag gtccgacgtc aatcagataa tctgctgccc
    43921 acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt gccaagatca cgcattttct
    43981 ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa tccgatcgat ggttcatcta
    44041 aaatatacaa aactccagaa agattcgatc caatttgtgt agccaatctg attcgctgcg
    44101 cttctccccc agataatgtt ccggaagcgc gactcaaagt tagatagtct agtcccacat
    44161 tttccaagaa tgaaagtctg ttatttactt cttttaaaat cggtttggca attactttct
    44221 cttgttcaga taacgttaag tttgagaaga aatctactgc tttttgaata gacaactcat
    44281 ttgtttgccc gatattcgtt ccattgattt gaacagatag agcttgggga ttcaatcgat
    44341 aacctttaca tgtctgacaa gttaattctg tcatataaag acgcatttgc tcacgcgtga
    44401 aatcactgtt cgtatcatga taacgacgct cgatattctt caatacccct tcgaaagtcg
    44461 tttctacatc gcgaacgcca ccaaaatcat tttcataatg gaaatggaaa agatcatctg
    44521 tcccattcaa gatgatatcc tgatgctctt ccggtaattc ttcgaatggt gtatctaaat
    44581 cgataccgaa actttcacaa gcctgcgcta acatttgagg ataatactga gagctgattg
    44641 gattccatgg aacgatagca ccttgactaa gtgtcttcgt tctatctgga atcactaaat
    44701 cttgatcgac ttctaatttg acacctaaac catcacagtc tggacaagct ccaaaaggcg
    44761 cattgaatga gaacagtctt ggttctagtt cgccaacagt aaatccgcaa taaggacagg
    44821 catagtgttc gctaaacaac atttcttctt caccgatcac gtcaaccaga gcatagccgt
    44881 ctgccaatcg cagagccgcc tctaaagaat caaatagacg agaacgaatc ccttctttca
    44941 caatgatccg gtcgatgacg atcgcgatat catgtttttt gtttttctcc agctcaggca
    45001 cttcacttaa atcatagatc tcaccatcga ctcgaacgcg gacgtatccc tctttttgga
    45061 ttcgttcgaa tattttttta tgttgtcctt ttttctttac cacgatcggt gccaacactt
    45121 ggatctttgt gcgctcaggc aaagccatta cttgatctgc catttgatct actgattgac
    45181 tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc tacacgtgca aacaacaagc
    45241 gtaaatagtc attgatttcc gtcacagtac ctactgtcga ccgaggattt ttacttgttg
    45301 ttttctgatc aatagaaatc gccggactca atccgtcgat gctatctacg tccggcttat
    45361 ccatttgtcc taaaaactga cgagcataag cagaaaggct ctctacgtat ctccgctgcc
    45421 cttcagcgta cagcgtatca aatgctaggg aactttttcc agaaccggac aaacctgtaa
    45481 cgaccacgaa tttatctcgt ggaatcgtta catctatatt tttcaagtta tgggcacgtg
    45541 caccgtgaat cgtaatttta tcgttagcca tgttgtcctc ctactttgaa gcttttaatt
    45601 ctaaaatcat atctcgcaca ttagcggctg tttcaaaatc caatgctttc gccgcatttt
    45661 tcatttcttt ttctaatttc atcaataacg tatctttttc ttctttcgtc atttcttcat
    45721 acgagacttg taccgcttct tttgtatcat cttccgattc tttcgtaata gaaatcaaat
    45781 ctcggatttc tttaatgatc gtttttggaa cgatcccatg ttcttcattg tacttctgct
    45841 gaatcgtacg gcgtcttgat gtttcatcca tcgctagacg catagaatct gttactttgt
    45901 ctgcgtacat gattacttta ccttcttcat tccgagcagc tcgtccaatc gtttggacta
    45961 aggaacgttc actacgtaaa aagccttctt tatccgcatc caagatagca acaagggaaa
    46021 cttcaggtac gtctaatcct tcacgaagca aattgattcc aactaacaca tcaaattcac
    46081 ctaatcgaag atctcgaatg atttctgttc gttccagcgt tttgatatca ctatgcaaat
    46141 atttgacttt gatccctaat tctttgaaat aatctgtgag gtcttctgcc atttttttcg
    46201 tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg ttcatttatt tcgcctacta
    46261 gatcatcgat ttgtcccatg attggacgaa cttcgatcac tgggtcaagc agccctgtcg
    46321 gacggatgat ttgttggata acggtatctg tttgttcatg ttcatatggt cctggcgtcg
    46381 cagagacata aatgatctga ttgacatgtt tttcaaactc ttctaaccga agtggccgat
    46441 tgtctaaagc agatggcaaa cggaaaccgt aatctactag catctgtttt ctcgctcggt
    46501 caccattata cattcctctg acttgaggca tggttacgtg cgattcgtcc gccacgatca
    46561 aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg ttcaccttct ttacgtccgt
    46621 ccatatgccg agaataattt tcaattcccg acgtgtagcc catttctaac atcatttcaa
    46681 tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag aagtttgttt tcatttcgca
    46741 agacagttaa acgctgttct aattccgctt tgatattcgc tacggcatgc tccatatgtt
    46801 catcattcgt cacaaagtga gtcgccggga agattgccac gtgttccgtt tctcctaaaa
    46861 cttcacctgt cagtgcattg acttctcgga tacgttcgac ctcatctcca aaaaactcca
    46921 cacgtaaggc acgttcatct cttgaagccg gaaaaatctc cacgacatca ccgcgcactc
    46981 gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa ttggatactg actaagtcac
    47041 gaatcaactg attgcgatct agttctgctc cctgtcgaat cgaaactact tgtttttggt
    47101 actcaaaagg cgaacctaat ccaaaaatac atgaaacgga tgcaatgacg atcacatcat
    47161 ttctttctaa taatgaacta gtagccgaat gtcgtaattt gtcgatctca tcatttacac
    47221 ttgaatcttt ttcgatatac gtatcgcttg aaggcacata agcttcaggc tgataataat
    47281 cataataaga aacaaaatat tcaacagcat tgttagggaa aaattccttg aattccccgt
    47341 acagctgacc agccaatgtt ttgttatggg caataattag tgttggttta ttcactttct
    47401 caataagatt ggaaatcgta tatgttttcc ctgttccagt cgcacctaat aagatttgtg
    47461 cttttttccc gccgactacg ccatccacta attggttaat cgcttcaggc tgatcaccag
    47521 ccggttggta tttcgagaca agctcaaagt gtcgggaggt atctctctca atcattcttc
    47581 gtcctccttt tcattcattc cttttcattt tacgaccaat aaaaagaaaa ggaaagcaaa
    47641 ggcattcctt actaaaaaat tataccacac cgaacatact ttcgccactt ttttatctta
    47701 ttatgcatgt tacgttttat aacataaaat gttagaaact cgcatttttt tcattttttc
    47761 gagcttacag tcttgtctga ttcagcactt ttcgcctata ataataaaat attggaaatt
    47821 taggaggttg attatgaaaa gaaagcattt atttcactca tttgttttct taatgacagg
    47881 acttgtgaca cttgcgtttg gacatgttgc acaagctgac aacactacaa cggcagagtc
    47941 aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa gacgtatacg taattgccag
    48001 tgactctgct tttgctccat ttgaatttca aaacgcaaag ggcgagtacg aaggcatcga
    48061 tgtaaaattg atggaaaaca tcgccaaact gcaaggattt aacatccgaa tggactaccg
    48121 tggtttctct gctgctttgc aagctttgga atcaggacaa gcagacggaa tgattgccgg
    48181 aatgactgta acagatgaac gaaagaaaag ctatgatttt tctgattctt actttgatag
    48241 cgggatccaa ttagctgtca aaaaaggaga tacttcgatc aagtcttatg aagatttgaa
    48301 aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct gaatttttaa gcgaccatga
    48361 atctgaatat ggctattcta tcaaatattt tgatgctgct gatcaattgt acgacgggtt
    48421 aaaagttggc gcaatcgatg caatgatgga tgattatccg gtaatcggtt atgcgatcaa
    48481 acaaggacaa gaattggaaa caccgatcaa aaaagaatct ggcggaaaat atgcatttgc
    48541 cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc aatgaaggat tgaaagaatt
    48601 aaaaagaaca ggcgaatacg ataagattat taaagaatat gtagcggatg gttcagaaac
    48661 ttccagtgag tcttccactg ctgatgaatc aacatttctt ggacttatcc aaaacaacta
    48721 tcaagcattg ttgaaaggac tttggagaac aattctccta actttgatct cctttgcttt
    48781 ggctatagta atcggtgtga tttttggatt attcagtgta gcaccaatac gcgctcttcg
    48841 cacaattgct agtatttatg tcgatatcat ccgaggaatt ccaatgatgg ttcttgcgtt
    48901 ctttattttc tttgggttgc ctggtatcgt tggatttacg attcctgact tccttgctgg
    48961 tgttatcacc ttgactctta acgcaagtgc ttatatcgca gagattgttc gaggcgggat
    49021 caacgccgtt cctgtcggac aaatggaagc ttccagaagt ttaggtcttt cttataatcg
    49081 gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc atgatcccat cattcgtcaa
    49141 tcagttcgtt atctcattaa aagacacaac gatcatttcc gcaatcggtg tagtcgaatt
    49201 gctgcaaact ggtaagatca ttgttgcacg tacaacacaa agtacgtatg tttacttgat
    49261 cattgcgatc atgtacttga ttttgatcac agcattgaca aaattagcaa aagttttaga
    49321 aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga gcatttagtc aaaaaatacg
    49381 gcgataacac agtattaaac gacatcaatg tgtccatcaa cgaaggagat gtcgtctgta
    49441 tcattggccc ttcaggttca ggaaaaagta catttttgcg ttgcttgaat cgtttagaag
    49501 aaccttctga cggtgaaatt attattgacg gcgcccactt gatggataaa agcacagata
    49561 tcaacttggt gagacaacat atcggaatgg tattccagca ctttaaccta ttccctcatc
    49621 tttctatttt ggaaaacatc attttagcac cgatggacct gaagaaagaa tctagagaag
    49681 aagcagagaa aaaagcaatc aaattgctgc agacagttgg tctagaagat aaaaaagata
    49741 tgtatcctga aatgctttcc ggtggacaaa agcagcgtgt ggctattgct cgtgctctag
    49801 cgatgaaccc tgacatcatg ctttttgatg agcctacatc tgcactcgac cccgaaatgg
    49861 tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca aggtatgacg atggttatcg
    49921 ttacgcacga aatgggcttc gctaaagaag tggccaatcg tgtcatgttc attgacgacg
    49981 gaaacttttt agaagacgga acgcctcaac aaatctttga aaatccgcaa aatgaacgga
    50041 caaaagattt cttgaataaa gtattaaata tttaataaaa accaaaaatg cccggtcgct
    50101 tcatgacgac cgggcatttt tagtttcttg ttttttcact taactgtacc agccgcatcg
    50161 cttgttctcc tgcacaaaca agcaatcgct ctacctgttc catcgcataa gaaagtacca
    50221 tcggttcatt gacgatatca agtacatagt cgataccacc ttcagaaact atgatgatta
    50281 tcttattcta aagctggctt ttttgttggt acaaacatga caactacaaa aaaaccaggc
    50341 gaacgtatga ttgttcgtct ggtttttaat tattaatgac ttagcgtttt tactagaaat
    50401 agaaaatttg ggcaagtcta ctttatattt ttactagaaa aactacttcc gctttttccc
    50461 gccaaaagca tctttaaact tatcgaagaa accgtcttct tgctgttctt caactttgat
    50521 gccgccagct tcagcgaatg cacgtaatgc ttctctttgt tgttcattta gatttttcgg
    50581 tgtgatcagt tttactttga catgttggtc gcctgttgca ccaccacgta acttcggagc
    50641 gccttttcca cgtaaacgga agttcgtacc tgtctgtgtt ccagcaggga tcttcaattt
    50701 cacatctcca tggacagttg gtacttggac ttcatccccc aaagctgctt gtacaaaact
    50761 taaaggcaat tcatagtaaa tttcagaacc ttcacgatca aagatatcac tttcttctac
    50821 acgaaaaacc acatacaaat caccaaatgg tccgccgttt tctccagctt ctccttgacc
    50881 agctaaacgc atttgttgtc cttcttctac accagctggt acattcactt taactgaatg
    50941 tgctttcttc tcatgtcctg agccatgaca ggttggacaa ggttctttga tttctttccc
    51001 tgtgccatga caaacatcac aagtttgacg gctcatcact cgtccaagtg gcgtttggcg
    51061 ttctacattg atcgtacctg caccatgaca tttatgacaa gtagttggtt gtgtgcctgg
    51121 ttttgcaccg tttccaccac aagtatgaca gatttcttcc cgattatact tgatttcttt
    51181 ttctacacca aaaatcgctt cttcaaaaga caagtcgacc gtatattgca aatcagcacc
    51241 ttgacgaggc gcatttggat cgactgaccg accgcctcct ccaaagaaag agtcgaagat
    51301 atcttcaaaa ccaccgaagc cgccaccaga gaagccgccg aatccaccaa atccgccacc
    51361 agctccacca gcaccgtaat ttggatctgt gcctgcatgc ccgtattggt cgtatgcagc
    51421 gcgtttttgc gggtcactta aaatctcata tgcttctgat acttctttga atttttcttc
    51481 cgcatcaggt tctttattga tgtcaggatg gtattttttc gaaagcttac gataagcttt
    51541 ctttatttca tcttctgatg ctccttttga gagccctaag acttcataat aatcacgttt
    51601 tgttgccata ggctttcctc ctattggttc tcatcatttt cacgtcgctt gttatcatat
    51661 catattttgt ttgaaacaaa aaccgatact tcaaactatt tttcaaaaga aaaagccaaa
    51721 gtagcaactt tggccttctc tcgaaattca gtgaactgat tatttatcgt cgccatcgac
    51781 ttcttcaaaa tcagcatcaa cgacatcatc tgctccgcct tgagctgctt ctggattttc
    51841 ttgagcttgt tgttgtgcag cttgttcgta tagtttaaca gtcaagtttt gaacgatttc
    51901 gttcaatgca tcgcgtttag ttttcatttc ctcgatgttg tttgcttcaa cagcggcttt
    51961 taattcgtca cgagctgttt ctgctttttt cacttcttct tcgtccactt tgccttctaa
    52021 ttctttcaat gttttgtcca cagagaatag caatgcgtct acatcattac gtaggtcaac
    52081 ttcctcttta cgagctttat ctgcttcagc gtttgcttct gcatctttca ccatacgttc
    52141 gatttcttcg tctgttagac ctgaagaaga tttgatcgtg attttttgtt ctttttgtgt
    52201 acctagatct ttcgcagata catttacaat cccgttttta tcaatatcga atgttacttc
    52261 gatttgtgga ataccacgag gagctgctgg aatatctgtc aattggaatc ttcctagagt
    52321 tttgttgtca gcagccattg ggcgttcacc ttgtaataca tggatgtcta ctgctggttg
    52381 attatctgcc gcagttgaga atacttgtga tttacttgtt ggaatcgttg tattgcggtc
    52441 aatcaattta gtgaacactc cacccattgt ttcgatacct aatgacaatg gtgtaacgtc
    52501 aagtaaaaca acatctttca catcaccagt gatcacgcca ccttggatag cagcacccat
    52561 agcaactact tcatcagggt taactgattt gtttggttct tttcctgttt ctttacgtac
    52621 tgcttctaca actgcaggga tacgagtaga tccaccgact aaaataactt cgtcgatttc
    52681 tgattgtgac agacctgcat cttttaacgc ttgacgtact ggtacttttg tacgttctac
    52741 taagtcagct gttaattcat caaattttgc acgtgtcaat gtcatttcca aatgcaatgg
    52801 tccagattct cctgctgtga taaatggcaa gctgatttgt gtgcttgata cgccagataa
    52861 atcttttttc gctttttcag cagcgtcttt caagcgttgt aaagccattt tgtcttgaga
    52921 caagtcaatg ctgttttctt ttttgaattc tgctaccatg tagtcgatga ttttgttatc
    52981 aaagtcgtcc ccacctagat ggttgtcgcc agctgttgac aatacatcaa agacaccgtc
    53041 tcctagttca aggatagata cgtcaaaagt accgccacca aggtcgaata ctaagatttt
    53101 ttcgtcgcga tctgttttat ctaaaccata ggcaagagca gctgcagttg gttcattgac
    53161 gatacgttct acttctagac cagcgatctt accagcatct tttgttgctt gacgttgtgc
    53221 atcattaaag taagctggca cagtgataac tgctttttcg actttttcac ctaaatagtc
    53281 ttctgcgaat cctttgatat attgaaggat cattgctgag atttcttgtg gtgtatatga
    53341 tttaccttct acatctactt tgtaacctgc ttcaccgata tggcgtttga ttgatgaaat
    53401 cgtatttggg tttgtcacag cttgacgttt tgcaacttcc cctacttgga tttctccatt
    53461 tttgaatgat acaacagaag gtgttgtacg gtttccttct gggttagcga tgatttttgc
    53521 ttcgtttcct tccaatactg ctactgcaga gttagtcgta cctaagtcaa taccgataat
    53581 tttactcata attaacgtct ccttctttaa ttgaaagtgt aagttttata tccgcacaat
    53641 tattgtgcaa caatgaccat agttggtctt aacacgcgat catgtaattt gtagccttct
    53701 tgtagtactt ctacaatcgt atcggctggt gtatcttcag tcgctggaac agtttgtact
    53761 gcttgatgaa gattaggatc aaaggcttct cctttagcag ggattttttc aattccttct
    53821 tcttccagtg cgttacgcaa actttccaag accatttcca cgcctttttt caaacctgcc
    53881 ccttgatcat ctgacacttc tgtggctaat gcacgttcca ggttgtcaat tgatggaagt
    53941 aattttttgg ctaaatcttg tgaacggtat ttttgtaatt gctcacgctc attttttcct
    54001 cgatttgcca tatttgcgat ttcagcacga gcccgcaagt atttatcttc catttcttct
    54061 aactcagctt ttaatttcgc tgtttcgaat tcttccgctt cagcttctga aatacccgaa
    54121 tcttctgcag cttcgacatc gatttccggt tctggctgag catctgtcat ttctttatct
    54181 agttcttctt gattttcttt cacgatacag tcttcctttc attattcagt cagctgtttg
    54241 attgctctta ctaagaaaga gagaacgatc agctgcaaat agcttcgtta ttcacattac
    54301 gagtttgaca agtcaagcga gcggtagtaa tcagcaagtt tagctgctag ttctcttcga
    54361 taaacatcga ctagaccgaa catccgagaa taaggcatac tagtaggccc taacaaggct
    54421 attgtacctc ttccatgacc atctatctcg taacttgctt gaatcatact catattctgc
    54481 aataaatcat tgcctaattc agcaccaatc ttgatatgaa tattgctatc catcggagtg
    54541 atcatttgtg taagtccgtc tgagtctttc ataaaagaaa acatcgattt gaactgatca
    54601 agatcttgac ggggctcata atccaagaga ttcatttgtc cactgacata aaccttttct
    54661 tcaaaaacat gaccaagaac ggtgtcaaat aactgagaca tcccttctgt cgtctggaaa
    54721 tatttatgaa gaatcatcgg aatctccgta cgaagtttat ggtacaccgt catcagagga
    54781 tctccgacta gacggtcgtt tacaatctgt accatttttt ctagttcctg acttcccata
    54841 tattcgggaa gagtgaacac ttggctttct acattcccca tatctgttac gacgatagca
    54901 ataatctgtc gattattcaa gggtacgata cgaaagccag tcagtcggcg gtcttttatt
    54961 tcaggtccca atgaaaaagt cgtatagctt gtcagctcag ataatatctc agcagattgc
    55021 ttaataatct cattgatttc atgaaattct ctgcctagag actgacgaat cagcagcaaa
    55081 tcatcttctg ctacgcgagc aggttttaac aaatgatcca cgtaataacg atagcctaaa
    55141 gcagatggaa tccgcccaga agaagagtga gtcttcaaca gcaatccttc atcttctaat
    55201 gctttcatat cattgcgaat cgttgctgag ctggccttga ctccctcaga cattaatgtt
    55261 ttggaaccaa cgggcacgcc actactcgta tagtgttgga tgatcagacg taaaatgtcg
    55321 ttttgtcgct ttgttaacat cttcctcacc tctcattagc actcatatat tctaagtgct
    55381 aattcactta taaatatagc aagaagccct tctcctgtca agagaaagac tccatttttt
    55441 agcactctaa acacaagagt gctaattcta aataattgat gtaaatccag ttatatcaat
    55501 acttcaatac ctcattgtca aactttctaa actttttctt ttcaagtttc gataaaaaac
    55561 aaatattctt caaactattt ttcaaataaa aagcgctcaa acacattgtt ccccaaaaaa
    55621 gtccctcttg gtgtcaaata aacacggtct tcttcattaa caagtaaccc ttcttgctct
    55681 aattccctta gcacatcacc gtacaccgat tcaattgttt tttgatagcg atcatagaaa
    55741 tgttttttag taatccctgt tttttttctt agtcctaaga acatttcttc ttctatttga
    55801 ttttttcgtg tcaatttttc ttcttccaat accgggcgtt catttgctcg taatgggcgt
    55861 aaatagtgtt ggattggtcc tttgttgcgg taacgaatat ctccaagata accgctagca
    55921 ccagctccaa acccaaaata gtggtcattg ttccagtaca tgaggttatg ttgagattca
    55981 tgtccggaga gcccaaagtt acttatttca tattggtgac gtccatgctt ttccattgct
    56041 tcaatcgtct cttcaaacat tctggtttcc gtttcctgat caggcagttc gagtcttcct
    56101 tgtcgtaccc agttcatgaa cattgttttg ttttcgagga tcaatgagta caaagaatag
    56161 tgaggcagat ccaaagccaa agctttttcc aaagtatccc gatatccttc tagtgtttgt
    56221 cccggtaatg cataaatcaa atcgatgctt acattggtaa aattttctgc ttctaaaaat
    56281 tgcattgttt gatacacatc ttctgcagta tgtttacgtc caattttttt caataagcga
    56341 ttgtcaaatg tctgcactcc catggataaa cgattgacgc catagttttt catcacttga
    56401 agtttttcac gggtcaaatc accaggattc gcttctacag taaactcatt accattttga
    56461 aaaggcagga tctctctagc acctgataat agtcgatcaa gttgtttagc agataaagaa
    56521 gttggtgttc cgcctcctac ataaagtgtt tccaaattcg ctgtaggatg actttccagc
    56581 attaatctca tttcattcag cagcatttcc acatactcat ctactggctg cccttctaaa
    56641 aaaactttat taaagtcaca gtaatagcag atatgctcgc aaaatgggat atgaatgtac
    56701 gcagaaacct tgttttctgc gggatttatt gtttctatca ttgaaattct ttctccttac
    56761 gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc gttcaagcac caactcattg
    56821 aaataagccg catatttaga atcaaggaaa cctagctcta aaaatgcttg cttattgtca
    56881 tcggagctac acggtgcttt tacagcctct cgataaacgg taatcgcaaa gctgaccttc
    56941 ggcattaagt caaaatctgc aaaaatatga aaagctattt tcacagattc ctccttaagt
    57001 gctcagacca caacgctttg cttacaacct ctcatatacg catactaaac gtatttggcg
    57061 aacaaatcca ctaaaaaatc caagaatcga cttcttggat gattttttta cactcatgat
    57121 tcttcctaaa caattttgtc cttattttat tcataaaaaa atctagtcaa atctctcact
    57181 tcaagagttt taactagatt ccaattatta tttttttgta aaaataaaca gtctcttaat
    57241 catagacatt tcttcggtgc cctacttgaa tagcgataac aacgaattga ttatcttcta
    57301 tctctaccaa tacacgtatt tgccaatcag agctgcttct gaatttcggg cgtgaatttt
    57361 aaacggcagg tcattccagc ccaccaaatt cattaaggat tcttccatga atactgttgt
    57421 tttcccactt cttatccaat gtttatgggc aatttcagcc gtttgacgat tgtattcatc
    57481 ttctagttgt tccatcgaat agttttttag aagttcagat aaagtaatcc cataaaattc
    57541 agccatataa gaaagccatt cttttttggc gtcacttaca cgaaccgtaa tcatagccct
    57601 ataattgcct ctcttaaaga atctcatgca tttcctgatg tgattttaga aaaatttact
    57661 tgctaaaaaa ctgtgaaaat gtaggagcta gcatccttgt agtaaatgat cgagctcttt
    57721 attatacatt aaaaaagttc agaaattttg atgaagtagc caactacttt ttcatgagta
    57781 aagcaacact agaaaaagaa tcctccagta cttagaattt actggaggat gtacttttga
    57841 agaagcctat aggttattta aaaaatatta ctacaggaag gaacgagaat tacttcgtta
    57901 cttataatag aacttagtct tatatagaag gcatagctca cattagctta tttgtcctat
    57961 aaaaataaat tacggtattt tagaaaagct attttctctt ttttcaacga tcttcagcaa
    58021 tttttcttta tcgtcaggta actcaagttc atgaattcta gcatgacaat tgggacataa
    58081 cgcgatacag ttttcaatca catcttttcc accttgcgac aaatattcta tatgatgaca
    58141 atgtaaataa ggtacaccgt cttttataaa tggccctttt tcttggcaaa gttgacaaat
    58201 accttttgca agtcgtttaa catattcaga tacgaaggga ttacgtttat atatttttgt
    58261 ttttgagaag caaaactcct cttcgttaac atattttgca gccatttttt tattataatt
    58321 actaactttt cttgcttctt cctctaattt agcattagat aaattacgaa tatgtctagt
    58381 ttgattttct tcctggttat atatctttat cttcaatcaa ttgctcttga tttattaaag
    58441 ctaatggaaa tttatatact tttctgctgt tgcccgaact atctttttca ttaacagtat
    58501 atatttgtcc tgctaaatat acttctccca gatatgtgtg ttcttttggt ttaaatgttt
    58561 caaataaatg aatctttaca aaattacttt ttgaattggc taaaacttta ttttgattct
    58621 tatccaatac ttgatcccct tttagcccca taccggtata ttgtaaaata ccatttaacc
    58681 acttatcttt ataaacatta cttcctgttt gatcactaaa cagtacaaga gtattagttt
    58741 gatgagatct tctcattcca ccctgtttgg aacatttaaa cttctctgaa atttctttgt
    58801 tagtatatac tctaccaact tctaactcac ctaatatgtt tttagaccaa ttttctcgcc
    58861 atgtaataat cttatcaaga ttaaacattt ctccctcttt tggcatgcca tttctaatta
    58921 aattagctaa tacaccagta gaaatatcta atttttttag taattctttc ttagtaatca
    58981 atgttggatt cataacaatg cagctcctaa tgatatcaat ataattgcat tagataactt
    59041 gaaaaatcga aaaaaactca ttttgtgtca agttatctgt aatatataaa aatatcatta
    59101 t
//
LOCUS       DANJMA010000012        53773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000012 DANJMA010000000
VERSION     DANJMA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            10..732
                     /locus_tag="PWU31_000892"
     CDS             10..732
                     /locus_tag="PWU31_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1083454.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            790..2829
                     /locus_tag="PWU31_000893"
     CDS             790..2829
                     /locus_tag="PWU31_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL1083455.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2938..4089)
                     /locus_tag="PWU31_000894"
     CDS             complement(2938..4089)
                     /locus_tag="PWU31_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL1083456.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4243..4773)
                     /locus_tag="PWU31_000895"
     CDS             complement(4243..4773)
                     /locus_tag="PWU31_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL1083457.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4872..5222)
                     /locus_tag="PWU31_000896"
     CDS             complement(4872..5222)
                     /locus_tag="PWU31_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL1083458.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5379..6908)
                     /gene="ahpF"
                     /locus_tag="PWU31_000897"
     CDS             complement(5379..6908)
                     /gene="ahpF"
                     /locus_tag="PWU31_000897"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL1083459.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6918..7481)
                     /gene="ahpC"
                     /locus_tag="PWU31_000898"
     CDS             complement(6918..7481)
                     /gene="ahpC"
                     /locus_tag="PWU31_000898"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL1083460.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7625..8287)
                     /locus_tag="PWU31_000899"
     CDS             complement(7625..8287)
                     /locus_tag="PWU31_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1083461.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8471..10117
                     /locus_tag="PWU31_000900"
     CDS             8471..10117
                     /locus_tag="PWU31_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL1083462.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10108..11190
                     /gene="uxuA"
                     /locus_tag="PWU31_000901"
     CDS             10108..11190
                     /gene="uxuA"
                     /locus_tag="PWU31_000901"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1083463.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11298..12851)
                     /locus_tag="PWU31_000902"
     CDS             complement(11298..12851)
                     /locus_tag="PWU31_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1083464.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12999..14287
                     /locus_tag="PWU31_000903"
                     /pseudo
     CDS             12999..14287
                     /locus_tag="PWU31_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14413..15492)
                     /locus_tag="PWU31_000904"
     CDS             complement(14413..15492)
                     /locus_tag="PWU31_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1083465.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15595..16035)
                     /locus_tag="PWU31_000905"
     CDS             complement(15595..16035)
                     /locus_tag="PWU31_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1083466.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16092..16502)
                     /locus_tag="PWU31_000906"
     CDS             complement(16092..16502)
                     /locus_tag="PWU31_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1083467.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16682..17116)
                     /locus_tag="PWU31_000907"
     CDS             complement(16682..17116)
                     /locus_tag="PWU31_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1083468.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17216..18064)
                     /locus_tag="PWU31_000908"
     CDS             complement(17216..18064)
                     /locus_tag="PWU31_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1083469.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18453..20219
                     /locus_tag="PWU31_000909"
     CDS             18453..20219
                     /locus_tag="PWU31_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL1083470.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20321..21580
                     /locus_tag="PWU31_000910"
     CDS             20321..21580
                     /locus_tag="PWU31_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1083471.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21727..23028
                     /locus_tag="PWU31_000911"
     CDS             21727..23028
                     /locus_tag="PWU31_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083472.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23031..23882
                     /locus_tag="PWU31_000912"
     CDS             23031..23882
                     /locus_tag="PWU31_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083473.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23895..24710
                     /locus_tag="PWU31_000913"
     CDS             23895..24710
                     /locus_tag="PWU31_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL1083474.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24861..26027
                     /locus_tag="PWU31_000914"
     CDS             24861..26027
                     /locus_tag="PWU31_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1083475.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26257..26904
                     /locus_tag="PWU31_000915"
     CDS             26257..26904
                     /locus_tag="PWU31_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL1083476.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26988..27932
                     /locus_tag="PWU31_000916"
     CDS             26988..27932
                     /locus_tag="PWU31_000916"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL1083477.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28067..29518
                     /locus_tag="PWU31_000917"
     CDS             28067..29518
                     /locus_tag="PWU31_000917"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL1083478.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29539..30375
                     /locus_tag="PWU31_000918"
     CDS             29539..30375
                     /locus_tag="PWU31_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1083479.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30375..31034
                     /locus_tag="PWU31_000919"
     CDS             30375..31034
                     /locus_tag="PWU31_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1083480.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31160..31849
                     /locus_tag="PWU31_000920"
     CDS             31160..31849
                     /locus_tag="PWU31_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083481.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31855..32811
                     /locus_tag="PWU31_000921"
     CDS             31855..32811
                     /locus_tag="PWU31_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL1083482.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32855..33367
                     /locus_tag="PWU31_000922"
     CDS             32855..33367
                     /locus_tag="PWU31_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083483.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33623..34171)
                     /locus_tag="PWU31_000923"
     CDS             complement(33623..34171)
                     /locus_tag="PWU31_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1083484.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34260..35441)
                     /locus_tag="PWU31_000924"
     CDS             complement(34260..35441)
                     /locus_tag="PWU31_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL1083485.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35677..37185)
                     /locus_tag="PWU31_000925"
     CDS             complement(35677..37185)
                     /locus_tag="PWU31_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL1083486.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37344..37535
                     /locus_tag="PWU31_000926"
     CDS             37344..37535
                     /locus_tag="PWU31_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083487.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37738..38655
                     /locus_tag="PWU31_000927"
     CDS             37738..38655
                     /locus_tag="PWU31_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL1083488.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38648..39721
                     /locus_tag="PWU31_000928"
     CDS             38648..39721
                     /locus_tag="PWU31_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083489.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39869..40387
                     /locus_tag="PWU31_000929"
     CDS             39869..40387
                     /locus_tag="PWU31_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083490.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40368..40760
                     /locus_tag="PWU31_000930"
     CDS             40368..40760
                     /locus_tag="PWU31_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL1083491.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40836..41771
                     /locus_tag="PWU31_000931"
     CDS             40836..41771
                     /locus_tag="PWU31_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL1083492.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41904..42683
                     /locus_tag="PWU31_000932"
     CDS             41904..42683
                     /locus_tag="PWU31_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL1083493.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42676..42993
                     /locus_tag="PWU31_000933"
     CDS             42676..42993
                     /locus_tag="PWU31_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1083494.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43237..44847
                     /locus_tag="PWU31_000934"
     CDS             43237..44847
                     /locus_tag="PWU31_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1083495.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45004..45852
                     /locus_tag="PWU31_000935"
     CDS             45004..45852
                     /locus_tag="PWU31_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1083496.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46083..46562
                     /locus_tag="PWU31_000936"
     CDS             46083..46562
                     /locus_tag="PWU31_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083497.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46595..48823
                     /locus_tag="PWU31_000937"
     CDS             46595..48823
                     /locus_tag="PWU31_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1083498.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48952..50595
                     /locus_tag="PWU31_000938"
     CDS             48952..50595
                     /locus_tag="PWU31_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1083499.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50668..51213)
                     /locus_tag="PWU31_000939"
     CDS             complement(50668..51213)
                     /locus_tag="PWU31_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL1083500.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51236..51721)
                     /locus_tag="PWU31_000940"
     CDS             complement(51236..51721)
                     /locus_tag="PWU31_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1083501.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51711..52523)
                     /locus_tag="PWU31_000941"
     CDS             complement(51711..52523)
                     /locus_tag="PWU31_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL1083502.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52662..>53773)
                     /locus_tag="PWU31_000942"
     CDS             complement(52662..>53773)
                     /locus_tag="PWU31_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL1083503.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17906 a         9785 c        10381 g        15701 t
ORIGIN      
        1 ttatctattt tgttactgct tacattagta gttggctatt tttcgattga gtttatccat
       61 ggattttcgt ctgcaaaaca gagttcaact gtgaaaaagg tagagccgaa aagtatccct
      121 accacactaa atgtggcttt gattggttca gatgctcgtt cgaaagacga aaacggtcgc
      181 tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct
      241 atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct
      301 tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa
      361 gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt
      421 cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac
      481 aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac
      541 aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag
      601 cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt
      661 agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa
      721 gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
      781 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
      841 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
      901 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
      961 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
     1021 ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa
     1081 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
     1141 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
     1201 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
     1261 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
     1321 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
     1381 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
     1441 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
     1501 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
     1561 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
     1621 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
     1681 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg
     1741 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
     1801 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac
     1861 agtctaggag gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
     1921 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
     1981 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
     2041 tatgctcctg gatctacgtt taaaataatt acagcagcaa tcggtctcga caacggcact
     2101 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
     2161 ggatcttttc aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
     2221 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
     2281 tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
     2341 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
     2401 ggatatggac agggcgaact tctaattaat cctatccagc aagcagcaat gtattctgtt
     2461 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
     2521 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
     2581 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
     2641 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
     2701 ttgcttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
     2761 gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
     2821 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
     2881 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
     2941 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
     3001 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
     3061 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
     3121 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
     3181 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
     3241 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
     3301 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
     3361 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
     3421 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
     3481 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
     3541 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
     3601 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
     3661 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
     3721 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
     3781 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
     3841 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
     3901 taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
     3961 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
     4021 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
     4081 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
     4141 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg
     4201 attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt
     4261 tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa
     4321 tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat
     4381 caacaacaga atactactat aaacactcca acttttagct tttgtattca tgctgaagaa
     4441 caactggaac aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac
     4501 gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa
     4561 cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa
     4621 caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga
     4681 agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta
     4741 tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt
     4801 aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat
     4861 ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg
     4921 atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt
     4981 acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca
     5041 aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa
     5101 cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct
     5161 tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc
     5221 ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga
     5281 gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc
     5341 cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg
     5401 aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga
     5461 ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg
     5521 gtcactattt catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg
     5581 agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg
     5641 cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca
     5701 tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct
     5761 tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt
     5821 gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa
     5881 gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt
     5941 acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat
     6001 ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt
     6061 tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat
     6121 tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact
     6181 tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata
     6241 gctgcactac tagctgctgg tccaccgcca attactaata catcataagg ttctttttca
     6301 aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg
     6361 cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta
     6421 tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt
     6481 aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta
     6541 acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca
     6601 ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca
     6661 tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa
     6721 aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct
     6781 atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa
     6841 aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg
     6901 tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg
     6961 ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca
     7021 gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc
     7081 atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc
     7141 tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg
     7201 tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta
     7261 tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt
     7321 aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata
     7381 ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg atgataagca
     7441 tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata
     7501 attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt
     7561 atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa
     7621 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
     7681 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
     7741 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
     7801 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
     7861 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
     7921 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
     7981 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
     8041 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
     8101 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
     8161 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
     8221 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
     8281 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
     8341 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
     8401 acatagcgat ttttattgta taaaattcac ttgaatacaa aaactaatat gttagtttag
     8461 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
     8521 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
     8581 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
     8641 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
     8701 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
     8761 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
     8821 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
     8881 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
     8941 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
     9001 tattcttgct gcgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
     9061 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
     9121 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
     9181 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
     9241 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
     9301 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
     9361 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
     9421 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
     9481 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
     9541 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
     9601 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
     9661 tccaaatatt cctgatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
     9721 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
     9781 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
     9841 aggaaaacca ttcatcccga gtccagaccc actattgaca gactttcagc ataagctttc
     9901 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
     9961 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
    10021 agaaatgttc atcaagatgg tagcaggacc gaatgccgta cgaaaaacat tacaggatta
    10081 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
    10141 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
    10201 acattgatgg atataccagt aggagaggta tggcccaggg agaggatcaa agaactaaaa
    10261 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
    10321 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
    10381 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
    10441 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
    10501 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
    10561 gaatttgaat tgccgggatg ggaacctgag cgcttagctc atatcaaatc cttgcttgaa
    10621 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
    10681 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
    10741 tactcgattt ttggattacc tcgaatcgta aagaatcgag aagatttaga ttggttgtgc
    10801 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
    10861 ccagaaaatg atgtatatgg gattttagca gaattttgtc aaagagatcg aatcccattt
    10921 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accgcataaa
    10981 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
    11041 aacgaataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
    11101 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
    11161 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
    11221 aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
    11281 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
    11341 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
    11401 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
    11461 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
    11521 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
    11581 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
    11641 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
    11701 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaac
    11761 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
    11821 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
    11881 tcaggtataa acaaatcaaa tacagtacaa aaggactcca ccatttctct tgaataaaga
    11941 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
    12001 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
    12061 attggcagat taggtcgcaa aatatctaca gagggatcaa taaaaaagtc aatatattca
    12121 tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat
    12181 atttttaata gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct
    12241 tttttaaaaa aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct
    12301 gtagcagtac ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc
    12361 tcttttccag aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtacaat
    12421 gctaaaaatg cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt
    12481 tgctgactta aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga
    12541 taactgactg attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca
    12601 attccttcaa aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga
    12661 ttgagacgct caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac
    12721 atatggaaat gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa
    12781 agaaaaatat ctaattttgt attgcttttt ttcttcaaga gaaacgtttt aatcgcttct
    12841 tgagtttgca tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa aatattactg
    12901 tttttttcat aaatttagac aaaaaaacag taaacatata ggtagaatct ctactatctt
    12961 atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca accatcttaa
    13021 tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc cacacagagg
    13081 agatagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga attacctttc
    13141 aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga caaacagcga
    13201 ccatcaaagc aaaaattgac tattcaggag acttagcacc tatactacaa tcagctgtta
    13261 catttgagaa tgttgatccc gctttgtcga ttggctatga cgagaacact ttgactatcc
    13321 aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg ttaaaaccag
    13381 cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcatcat ttaacgccgt
    13441 atagtcagcg gcagacaatt gagcaagatt cagctatcga ctcaggagga gatcttgttg
    13501 aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc
    13561 cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac
    13621 cagagccaga caatgaatca aaacctgaaa taacccctgg agaaaaacca gatgtagacc
    13681 cagaagaaaa accagatgtg actccagaac cagatacgga ttctggaaac caaacagtac
    13741 cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagccc
    13801 cagaagaaaa accagatgta actccagaac cagatacgga ttctggaaac caaacagtac
    13861 cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc
    13921 cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac
    13981 cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa
    14041 atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata
    14101 gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac
    14161 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    14221 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    14281 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    14341 aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    14401 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    14461 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    14521 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    14581 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    14641 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    14701 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    14761 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt
    14821 taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    14881 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    14941 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    15001 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    15061 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    15121 atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca tgtcaaatgt
    15181 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    15241 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    15301 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    15361 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    15421 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    15481 cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta
    15541 aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc
    15601 ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc
    15661 caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg
    15721 tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt catcacccgt
    15781 ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt
    15841 accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca
    15901 taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga ggaggggagt
    15961 gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt gattaactaa
    16021 atatagatct tccatttttc cacctccgct gttttactat agtgtatcat agcggattta
    16081 cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct
    16141 tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa
    16201 agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa
    16261 taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc
    16321 aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg
    16381 gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga
    16441 ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc
    16501 ataaaattac cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg
    16561 aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct
    16621 ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct catcataaac
    16681 attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca cgatggacct
    16741 gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt
    16801 ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg
    16861 tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag
    16921 gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta ccatacaagg
    16981 taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat
    17041 caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca aaatcgattt
    17101 ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt
    17161 tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt
    17221 cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa
    17281 aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca
    17341 caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg
    17401 taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac
    17461 tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg
    17521 aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa ataaacagat
    17581 taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat
    17641 attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata
    17701 aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg
    17761 aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag cttccgaaaa
    17821 gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat
    17881 tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt aatattccta
    17941 gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc
    18001 ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt
    18061 tcatttattc acttccttaa ttcattaata atactcttat cttattataa cttacttttc
    18121 accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt atgtttcatt
    18181 cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta gaaaattgga
    18241 gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta gactaaaact
    18301 tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa
    18361 aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca cttatagtga
    18421 tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac
    18481 cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta
    18541 ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc ataacaactg
    18601 aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt
    18661 attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc catgttgttg
    18721 gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg
    18781 tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca
    18841 ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa
    18901 acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc
    18961 gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat tacttgacgg
    19021 accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat
    19081 acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa
    19141 acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc
    19201 atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct
    19261 ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac
    19321 cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac
    19381 taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa
    19441 aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt
    19501 ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga
    19561 tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg
    19621 catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg
    19681 tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca
    19741 acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat
    19801 tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg
    19861 ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg
    19921 agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga
    19981 aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta aatgatcgtg
    20041 aagataagct ctatttcaca cggaaactag agggacagat catctatgtt tactttaatc
    20101 agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc
    20161 agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa
    20221 aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata
    20281 agggtgtaac agcacgttga atacaaagga ggaaatgaag atgaaaaaaa gaagtgtgaa
    20341 acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa
    20401 tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga
    20461 ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt
    20521 gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg
    20581 gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg
    20641 acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga
    20701 caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct
    20761 tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga
    20821 gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt
    20881 tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt acggtggcta
    20941 tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt
    21001 agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga
    21061 cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat
    21121 cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc
    21181 caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg
    21241 ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac
    21301 gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca
    21361 agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata
    21421 tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga
    21481 aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc
    21541 agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat
    21601 ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta
    21661 tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc
    21721 attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca
    21781 cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc
    21841 gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga
    21901 ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc
    21961 actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac
    22021 gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa
    22081 gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt
    22141 ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat
    22201 tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt
    22261 tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc
    22321 gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt
    22381 gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca
    22441 tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta
    22501 ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt
    22561 gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc
    22621 tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa
    22681 gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc
    22741 acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat
    22801 ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag cgtaggcgga
    22861 ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct
    22921 ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg
    22981 tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat
    23041 ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt
    23101 taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt
    23161 caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta
    23221 ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg
    23281 tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct
    23341 atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga
    23401 ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt
    23461 actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga
    23521 aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag
    23581 ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc
    23641 aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc
    23701 gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa
    23761 accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat
    23821 tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat
    23881 aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa
    23941 ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat
    24001 ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga
    24061 ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat
    24121 tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga
    24181 cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt
    24241 ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc
    24301 gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt
    24361 tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt
    24421 atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt
    24481 cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga
    24541 gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt
    24601 ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc
    24661 ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag
    24721 cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc
    24781 tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag
    24841 tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt
    24901 ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata
    24961 cggacactcc tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta
    25021 caagtaacgc ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg
    25081 gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa
    25141 aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag
    25201 atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt
    25261 tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca
    25321 ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata
    25381 tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa
    25441 ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac
    25501 atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt
    25561 ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt
    25621 atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca
    25681 gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt
    25741 atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg
    25801 atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc
    25861 atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc
    25921 tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt
    25981 tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt
    26041 actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt
    26101 ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat
    26161 aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta
    26221 atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact
    26281 agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct
    26341 gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa
    26401 gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat
    26461 atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca
    26521 attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc
    26581 gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag
    26641 aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg
    26701 gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga
    26761 agcggtacgg caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc
    26821 aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta
    26881 ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt
    26941 agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa
    27001 cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca
    27061 tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga
    27121 gctgtacgat caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag
    27181 ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc
    27241 ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga
    27301 tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga
    27361 tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt
    27421 ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa
    27481 tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc
    27541 agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata
    27601 cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt
    27661 atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa
    27721 tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat
    27781 gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg gaaatttttt
    27841 ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg
    27901 aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt
    27961 cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg
    28021 ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac
    28081 agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat
    28141 acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt
    28201 cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat
    28261 cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc
    28321 tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc
    28381 gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac
    28441 agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg
    28501 atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag
    28561 tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc
    28621 ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat
    28681 tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag
    28741 tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa
    28801 aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga
    28861 ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac
    28921 agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg
    28981 cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa
    29041 agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc
    29101 tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca
    29161 tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga
    29221 agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt
    29281 agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac
    29341 acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga
    29401 aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca
    29461 aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca
    29521 gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt
    29581 tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat
    29641 gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat
    29701 tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa
    29761 cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt
    29821 tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa
    29881 aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa
    29941 gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc
    30001 aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat
    30061 gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat
    30121 gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg
    30181 gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct
    30241 ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa
    30301 atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag
    30361 tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc
    30421 ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag
    30481 gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg
    30541 tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc
    30601 tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta
    30661 cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt
    30721 caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata
    30781 tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc
    30841 tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa
    30901 agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt
    30961 attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatgc
    31021 atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg
    31081 tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat
    31141 atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata
    31201 ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca
    31261 aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg
    31321 ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg
    31381 acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc
    31441 tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc
    31501 tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc
    31561 tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga
    31621 gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa gacgttcaga
    31681 aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag
    31741 tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt
    31801 gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac
    31861 tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga
    31921 ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt
    31981 aaaaatgtga tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc
    32041 caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat
    32101 agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca
    32161 cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa
    32221 cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg
    32281 ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt
    32341 tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga
    32401 tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct
    32461 agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa
    32521 cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca
    32581 ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga
    32641 atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa
    32701 aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat
    32761 tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat
    32821 gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga
    32881 agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg
    32941 ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga
    33001 aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt
    33061 ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc
    33121 ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa
    33181 accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat
    33241 cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta
    33301 tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat
    33361 caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa
    33421 tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt
    33481 tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt
    33541 ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc tcggcttatc
    33601 ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt
    33661 cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca
    33721 actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc
    33781 cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga
    33841 ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac
    33901 aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag
    33961 catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag
    34021 aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag
    34081 taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag
    34141 aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag
    34201 tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtatat
    34261 tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa
    34321 attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca
    34381 ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt
    34441 gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat
    34501 tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg
    34561 attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg
    34621 ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc
    34681 atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg
    34741 aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca
    34801 tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg
    34861 atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta
    34921 gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt
    34981 tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat
    35041 tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat
    35101 ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc
    35161 aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca
    35221 acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc
    35281 gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc
    35341 acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct
    35401 aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact cctattccaa
    35461 attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt
    35521 acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag
    35581 gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta
    35641 atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga
    35701 agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt
    35761 tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata
    35821 agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt
    35881 gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc
    35941 aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc
    36001 atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac
    36061 ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg
    36121 aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc
    36181 gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt
    36241 gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac
    36301 aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg
    36361 ataaacagat tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc
    36421 aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat
    36481 catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt
    36541 caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt
    36601 ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg
    36661 agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc
    36721 ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac
    36781 cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata
    36841 gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg
    36901 caagatatcc atatcataag taatcccata atcaccacgg atcttattac gttcaatatc
    36961 tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat
    37021 tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt
    37081 cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga
    37141 ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta
    37201 agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc
    37261 aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata
    37321 gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac
    37381 actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac
    37441 aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca
    37501 ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt
    37561 cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt
    37621 ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa
    37681 tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg
    37741 aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat
    37801 caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat
    37861 ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc
    37921 cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat
    37981 gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa
    38041 tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc
    38101 acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt
    38161 ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac
    38221 aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta
    38281 gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc
    38341 ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta
    38401 tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa
    38461 aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa
    38521 gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat
    38581 gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt
    38641 tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga
    38701 tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga
    38761 agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa
    38821 tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga
    38881 aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt
    38941 agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc
    39001 atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat
    39061 cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa
    39121 ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca
    39181 aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc
    39241 gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt
    39301 gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat
    39361 attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa
    39421 tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt
    39481 gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat
    39541 ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat
    39601 aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc
    39661 tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg
    39721 attggataag ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc
    39781 agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact
    39841 tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt
    39901 attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc
    39961 aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt
    40021 catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg
    40081 ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac
    40141 agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg
    40201 aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta
    40261 ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt
    40321 ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa
    40381 tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct
    40441 atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca
    40501 cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg
    40561 atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt
    40621 cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg
    40681 ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt
    40741 tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat
    40801 tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa
    40861 tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg
    40921 gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt
    40981 gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt
    41041 tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc
    41101 ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat
    41161 cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg
    41221 aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt
    41281 ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag
    41341 acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa
    41401 ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag
    41461 ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa
    41521 gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata
    41581 taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg
    41641 gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt
    41701 ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt
    41761 ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt
    41821 tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg
    41881 ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt
    41941 tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct tgcggagtat
    42001 tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt
    42061 ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga
    42121 cattattgat ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg
    42181 gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg
    42241 aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg
    42301 ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt
    42361 ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt
    42421 tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct
    42481 caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag
    42541 caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag
    42601 ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac
    42661 aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc
    42721 gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc
    42781 tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa
    42841 gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg
    42901 attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga
    42961 cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga
    43021 ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg
    43081 atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt
    43141 ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga
    43201 tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga
    43261 acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt
    43321 ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat
    43381 gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg
    43441 attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt
    43501 tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat
    43561 ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta
    43621 gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga
    43681 tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct
    43741 gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc
    43801 atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt
    43861 atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt
    43921 tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca
    43981 ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg
    44041 atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta
    44101 ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga
    44161 acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa
    44221 atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg
    44281 actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa
    44341 agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc
    44401 ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa
    44461 gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg
    44521 atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg
    44581 tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt aaaaactttc
    44641 cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga
    44701 ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc
    44761 ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt
    44821 aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa
    44881 acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct
    44941 aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa
    45001 cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac
    45061 catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa
    45121 ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct
    45181 actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa
    45241 gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca
    45301 ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc
    45361 actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag
    45421 tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta cgcttatctg
    45481 attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg
    45541 attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta
    45601 ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga
    45661 agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa
    45721 gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt
    45781 ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt
    45841 tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atattccaac atcatcttgg
    45901 actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt atttctatca
    45961 atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc atatagttta
    46021 gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga ggagttaaaa
    46081 taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa gactatgata
    46141 aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg gctttcactg ggccttgctc
    46201 agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat actggagaaa
    46261 aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta gcgatcttag
    46321 ctgcacaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt tatgatccag
    46381 aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta gaagtagata
    46441 ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta caaaaagacg
    46501 gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag ctaggatatt
    46561 aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac taacagaaaa
    46621 ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa gccatcggac
    46681 aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag atcctgaaat
    46741 gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac aattagccaa
    46801 agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg cagatatgga
    46861 caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg aacaagacca
    46921 agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga cactgatcca
    46981 gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc tagttcaaag
    47041 gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg aaatcgttcg
    47101 agcgattgaa aaaggatatt tattagagat tcaagagccg actgtcatcc gagatgcctc
    47161 tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt taccgacagg
    47221 aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa attaccaagg
    47281 aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa aaatggatct
    47341 tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc aaaaacaaga
    47401 acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa aagctaacgc
    47461 catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct ataagcaagg
    47521 acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca ctgattcaga
    47581 tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg aacttcagga
    47641 catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag gaagaaccat
    47701 tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa ttctgcgctt
    47761 agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga aacaagaaga
    47821 aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta gcgatatcga
    47881 aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt ctgcttctga
    47941 catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc gacaaatcat
    48001 gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt tccaaggaaa
    48061 aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat cacttagccg
    48121 gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag gaaaatacga
    48181 aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt ttgacaaaaa
    48241 gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg aatctgggtc
    48301 aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca ttgccgaatt
    48361 ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc gttcgtcaag
    48421 agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat ggttaaatga
    48481 aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac ttcgtttagc
    48541 agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata ttagtgacgg
    48601 tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata tccagtctgt
    48661 tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg gccaagacaa
    48721 agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg acttgaatga
    48781 atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata aagctcaacg
    48841 aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca aggatacttc
    48901 taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga tatgatacaa
    48961 gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag tttcaaagat
    49021 agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa ttatttgaaa
    49081 gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt tgtcgataat
    49141 ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat ggaagatttt
    49201 gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga tcttgtcatc
    49261 aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa agaaagcccg
    49321 tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa taattggaca
    49381 tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta ttatctgcat
    49441 gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa agaagaacta
    49501 ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt agatgcgatt
    49561 tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt ctcacccttt
    49621 caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt tgaagagtac
    49681 gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc acaatgggta
    49741 gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat ctggcagcaa
    49801 gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc ttggcaaact
    49861 tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt tcctcgtgcc
    49921 gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa agcaatcgct
    49981 atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga aatcggaatg
    50041 acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa aagactttat
    50101 aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat tcgtgaaaca
    50161 acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc tggtttttct
    50221 acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt agaacaacaa
    50281 gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat tagacaaacc
    50341 aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc acaactttat
    50401 gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt aacagaatca
    50461 cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact cttgtcaaat
    50521 tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc tcggttatat
    50581 aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc gaggaagttt
    50641 ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac cgatatattt
    50701 aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac gaggtctgac
    50761 aggtacttgg acatgtttga tatgcatacc gattgatgtg tcaccgatat cgataccggc
    50821 ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag cagcaacaga
    50881 acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat tttttgcttc
    50941 tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt ggacagctag
    51001 cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt gaccaacttc
    51061 cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc atcctaaaac
    51121 aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta tttcagtcaa
    51181 ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt ttcaattaat
    51241 attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca agtcccattc
    51301 caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc caatcatata
    51361 aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat aaaagcccta
    51421 atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct tgtaacccgt
    51481 ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg atcaatagac
    51541 cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca gcggtataga
    51601 tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc aaaattgtta
    51661 atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc ttagttttca
    51721 tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg atcgttcatc
    51781 agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac tgctttggta
    51841 aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca accggccccg
    51901 tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc aattgaatag
    51961 agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac aatgtttttt
    52021 gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga agagggaaga
    52081 gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt aacaagtgga
    52141 aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc agtttctttg
    52201 aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat gaagtctcga
    52261 acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc taatgaagtc
    52321 attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga ttccagcata
    52381 aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc gaatagacca
    52441 aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg atcagaaccg
    52501 ccaattgtca agattctttt cataacattt tcctccatga ataagagtat aacgaaattt
    52561 tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa aaaaacaaaa
    52621 agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt ttgtgatttt
    52681 aattctttct cgatttgaag ccgttgcact ttcattgtag ctgtccgcgg gattgcatca
    52741 aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc ccaccatcga
    52801 ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga atgattttta
    52861 tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa gtcttcaata
    52921 gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa aaataggtgg
    52981 ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata aacattttcc
    53041 tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag taattgtata
    53101 tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg agcgcttgtc
    53161 atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact ctctagtgta
    53221 tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag aaataccgca
    53281 tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat agcatcaaaa
    53341 gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc agtaaaacgc
    53401 cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc ttccaacata
    53461 tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc catggacatc
    53521 aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa agcgattaat
    53581 ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca tcccatagaa
    53641 tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg ggtcatataa
    53701 gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg ttccattaaa
    53761 taaagaatag gaa
//
LOCUS       DANJMA010000013        51881 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000013 DANJMA010000000
VERSION     DANJMA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(253..1056)
                     /locus_tag="PWU31_000943"
     CDS             complement(253..1056)
                     /locus_tag="PWU31_000943"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL1083504.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1076..1918)
                     /gene="kduI"
                     /locus_tag="PWU31_000944"
     CDS             complement(1076..1918)
                     /gene="kduI"
                     /locus_tag="PWU31_000944"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL1083505.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1934..2941)
                     /locus_tag="PWU31_000945"
     CDS             complement(1934..2941)
                     /locus_tag="PWU31_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL1083506.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2948..3592)
                     /locus_tag="PWU31_000946"
     CDS             complement(2948..3592)
                     /locus_tag="PWU31_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL1083507.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3692..4450)
                     /locus_tag="PWU31_000947"
     CDS             complement(3692..4450)
                     /locus_tag="PWU31_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL1083508.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4577..5728)
                     /gene="fucO"
                     /locus_tag="PWU31_000948"
     CDS             complement(4577..5728)
                     /gene="fucO"
                     /locus_tag="PWU31_000948"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL1083509.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5810..6121)
                     /gene="rhaM"
                     /locus_tag="PWU31_000949"
     CDS             complement(5810..6121)
                     /gene="rhaM"
                     /locus_tag="PWU31_000949"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL1083510.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(6123..6968)
                     /gene="rhaD"
                     /locus_tag="PWU31_000950"
     CDS             complement(6123..6968)
                     /gene="rhaD"
                     /locus_tag="PWU31_000950"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL1083511.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6982..8238)
                     /gene="rhaA"
                     /locus_tag="PWU31_000951"
     CDS             complement(6982..8238)
                     /gene="rhaA"
                     /locus_tag="PWU31_000951"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL1083512.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8235..9695)
                     /gene="rhaB"
                     /locus_tag="PWU31_000952"
     CDS             complement(8235..9695)
                     /gene="rhaB"
                     /locus_tag="PWU31_000952"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL1083513.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9848..10717
                     /locus_tag="PWU31_000953"
     CDS             9848..10717
                     /locus_tag="PWU31_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL1083514.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10843..11742)
                     /locus_tag="PWU31_000954"
     CDS             complement(10843..11742)
                     /locus_tag="PWU31_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083515.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11907..12731)
                     /locus_tag="PWU31_000955"
     CDS             complement(11907..12731)
                     /locus_tag="PWU31_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1083516.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12742..14037)
                     /locus_tag="PWU31_000956"
     CDS             complement(12742..14037)
                     /locus_tag="PWU31_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL1083517.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(14053..14532)
                     /locus_tag="PWU31_000957"
     CDS             complement(14053..14532)
                     /locus_tag="PWU31_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL1083518.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14538..15566)
                     /locus_tag="PWU31_000958"
     CDS             complement(14538..15566)
                     /locus_tag="PWU31_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL1083519.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15583..16689)
                     /locus_tag="PWU31_000959"
     CDS             complement(15583..16689)
                     /locus_tag="PWU31_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL1083520.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16918..19515)
                     /gene="adhE"
                     /locus_tag="PWU31_000960"
                     /gene_synonym="adhC"
     CDS             complement(16918..19515)
                     /gene="adhE"
                     /locus_tag="PWU31_000960"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL1083521.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19820..20200)
                     /gene="yajC"
                     /locus_tag="PWU31_000961"
     CDS             complement(19820..20200)
                     /gene="yajC"
                     /locus_tag="PWU31_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL1083522.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20297..21442)
                     /gene="tgt"
                     /locus_tag="PWU31_000962"
     CDS             complement(20297..21442)
                     /gene="tgt"
                     /locus_tag="PWU31_000962"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL1083523.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21659..22357
                     /locus_tag="PWU31_000963"
     CDS             21659..22357
                     /locus_tag="PWU31_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL1083524.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22525..25164)
                     /locus_tag="PWU31_000964"
     CDS             complement(22525..25164)
                     /locus_tag="PWU31_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1083525.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25783..26814)
                     /gene="queA"
                     /locus_tag="PWU31_000965"
     CDS             complement(25783..26814)
                     /gene="queA"
                     /locus_tag="PWU31_000965"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL1083526.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            27007..28182
                     /locus_tag="PWU31_000966"
     CDS             27007..28182
                     /locus_tag="PWU31_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1083527.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            28186..28827
                     /locus_tag="PWU31_000967"
     CDS             28186..28827
                     /locus_tag="PWU31_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083528.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            28824..29741
                     /locus_tag="PWU31_000968"
     CDS             28824..29741
                     /locus_tag="PWU31_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1083529.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            29738..30409
                     /locus_tag="PWU31_000969"
     CDS             29738..30409
                     /locus_tag="PWU31_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083530.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(30950..31438)
                     /locus_tag="PWU31_000970"
     CDS             complement(30950..31438)
                     /locus_tag="PWU31_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL1083531.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(31639..31905)
                     /locus_tag="PWU31_000971"
     CDS             complement(31639..31905)
                     /locus_tag="PWU31_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1083532.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(31905..32168)
                     /locus_tag="PWU31_000972"
     CDS             complement(31905..32168)
                     /locus_tag="PWU31_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1083533.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            32644..32829
                     /locus_tag="PWU31_000973"
     CDS             32644..32829
                     /locus_tag="PWU31_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1083534.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(32971..34794)
                     /locus_tag="PWU31_000974"
     CDS             complement(32971..34794)
                     /locus_tag="PWU31_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL1083535.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(35391..38096)
                     /locus_tag="PWU31_000975"
     CDS             complement(35391..38096)
                     /locus_tag="PWU31_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL1083536.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVVIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(38402..39349)
                     /locus_tag="PWU31_000976"
     CDS             complement(38402..39349)
                     /locus_tag="PWU31_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL1083537.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            39539..39808
                     /gene="rpsN"
                     /locus_tag="PWU31_000977"
     CDS             39539..39808
                     /gene="rpsN"
                     /locus_tag="PWU31_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1083538.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(39984..40346)
                     /locus_tag="PWU31_000978"
     CDS             complement(39984..40346)
                     /locus_tag="PWU31_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1083539.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(40357..41478)
                     /locus_tag="PWU31_000979"
     CDS             complement(40357..41478)
                     /locus_tag="PWU31_000979"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL1083540.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(41494..41844)
                     /locus_tag="PWU31_000980"
     CDS             complement(41494..41844)
                     /locus_tag="PWU31_000980"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL1083541.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(41974..43485)
                     /locus_tag="PWU31_000981"
     CDS             complement(41974..43485)
                     /locus_tag="PWU31_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1083542.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(43807..45159)
                     /locus_tag="PWU31_000982"
     CDS             complement(43807..45159)
                     /locus_tag="PWU31_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL1083543.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(45312..46388)
                     /locus_tag="PWU31_000983"
     CDS             complement(45312..46388)
                     /locus_tag="PWU31_000983"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL1083544.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            46584..48329
                     /locus_tag="PWU31_000984"
     CDS             46584..48329
                     /locus_tag="PWU31_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL1083545.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(48609..49046)
                     /locus_tag="PWU31_000985"
     CDS             complement(48609..49046)
                     /locus_tag="PWU31_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL1083546.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(49030..50403)
                     /locus_tag="PWU31_000986"
     CDS             complement(49030..50403)
                     /locus_tag="PWU31_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083547.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(50725..51213)
                     /locus_tag="PWU31_000987"
     CDS             complement(50725..51213)
                     /locus_tag="PWU31_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083548.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(51235..51837)
                     /locus_tag="PWU31_000988"
     CDS             complement(51235..51837)
                     /locus_tag="PWU31_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1083549.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        14634 a        11309 c         9352 g        16586 t
ORIGIN      
        1 gtaaagaaca cttttgtctc acgctctaaa cacgtacaaa cggcggaagt aaaagcaact
       61 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aattccttct
      121 tatttcttgg agttaaatgc ttctgtccca gcctcttttt tcgaataaat gatgtttaat
      181 cagctgtcct tcggtattcg ttcaatccgc tgatttcaca acctctccac taaacagtgt
      241 actaaagctc ttttaaggtt gttttccgat ataggcaagt atgcctccgt ccacatacaa
      301 gacatggccg ttaacgaaat cagatgcgct agaagccaag aagacagatg gtccagccaa
      361 atcgattggc tcgccccagc gtgctgctgg cgtacgtcca acgataaatt gatcgaatgg
      421 atgacgttca ccgttttctt gtgtttcgcg taatggagca gtttgcggtg tagcaatata
      481 acctggtcca attccattac attggatatt gtattgaccg tattcagaag cgatgttttt
      541 cgtcagcatt tttaatccgc cttttgccgc agcataagca ctgaccgttt cgcgtcctaa
      601 ttcactcatc atggagcaga tattgatgat tttaccgccg cctttttcga tcatatccgg
      661 aataactgct tttgaaacga tgaatggggc atttaaatcc acgtcgatta cttggcggaa
      721 gtctgccgca tccatttcga tcatcggtgt ccgtttgatg atccctgcat tgttgactag
      781 aatatcaact gaacctacat cttctttgat ctgttgaacc atcgcttgaa ccgcttcttc
      841 atcggtcaca tcgcagacat aaccttttgc atcgatgcct gcttctttat aatgcgctat
      901 accttcatca actgattctt gattcaagtt attaaatacg atttttgctc ctgctgtagc
      961 taaagagcgt gcaatctcaa atccaatacc atagacagca ccggtgacta atgctacttt
     1021 tccatctaaa cgaaacatat ccatactaaa ttccatcatt ttctctccct tatgcttatt
     1081 ttaattgatc catttgcacc atatccatgt ctgtataggt gatattttct ccgcacattg
     1141 cccagatgaa tgagtaattg cttgtaccta cacctgaatg gatcgaccag cttggcgaaa
     1201 taatggcttg ttcattgtct acaactaaat gttttgtttc atctggtttg cccatcatgt
     1261 ggaacacacg tgtatcttca ttggcataat caaagtagac ataagcttcc atacgacgtt
     1321 catgtgtatg acaaggcatc gtattccaag cgcttcctgg ttctaaaatc gtataaccca
     1381 tttgtaattg acagctttcg catacatttg gatggatgta ttggtagatt ttacgttcat
     1441 tcagtgtcag gccttcacca gtctccattg gttttacttg gtcgatactg atttttacat
     1501 ttggatattt atgatgagct ggaacagaac taatatagaa tttagctggg ttttcagcat
     1561 cttgagaaga aaatacaaca tgtcgtgttt cttttccgat atagtatccg tcttgttttt
     1621 tcatttcttc tttttgccca tcgatctcga tgaatcctgg tccaccgata ttgatcacac
     1681 ctagttcacg acgctctaag aagtaatcaa cacctagttc tttgtctaat gtgatttcta
     1741 attctttttc agtaggtgtc actccaccaa agatcattcg atcgttgtgc gtataagtca
     1801 aactgatttt ccctggttca aatacctttt ctactaaaaa ttcttttctc aattgttccg
     1861 ttgaataatg acggatatct tctggactgt gtgtataacg tgtttccatt tcttgcattt
     1921 tcttctactc cttctatcga atgatttttc cagagccgca tgctaaaaat tgtgcgactt
     1981 cttcttgact aaattggttg cagtccccat ggatcgtatg cttcaaggca gaagctgccg
     2041 tagcaaaatc aatgatttct tgcggagaca tattggataa aatcccatgg agcacgccac
     2101 ctgcaaaagc atctccgcca cctactcgat ccacgattgg ggtgattcta tggacttggg
     2161 attccacata ttcatggttc atccacaatg tccccttcaa ttcattttca cttgctgaat
     2221 gaacggtacg cttggtcgaa taaaacacct gaatattcgg gaacttttcc tgcatttttt
     2281 gataataata aattagaggt tgttcttctg cttctggcgc tttctcgata cctaacaaat
     2341 aaagagcatc catttttcca gctgagcaga tatctactaa aggtaaaagc tgatggatta
     2401 cttcgctggc ttcttgctgt gtccataatt ttccacgata gttgatatcg aagctgatca
     2461 ggcagccagc tttttttgca gcttctatca cctgtttcgt cattttttgc cattttttag
     2521 acaaagctgg ggtgattcct gaaacatgaa atatatctat tccttggaat aactcctcta
     2581 gcgaccattc caaatgatcc atttcagcaa aacttgatcc ggcacgatca tagatcactg
     2641 aggcggcgcg ttcgccacta ccagtttcca tataataagt acctagtctt gatccaccag
     2701 ataatacctg tttcgtatct actccataac attgcaagtg ctttttgact gcttttccca
     2761 atgcgttatc aggtactttt gtagcaaaaa tcgcttcatg tccgtaatta gctaaagaaa
     2821 ttgctacgtt tgcttcccct ccgccataat gggcagcgaa tcgttcactt gaatcgatac
     2881 gggttccctg ctctgtggac aatcgcaaca tgatttctcc gagtgtcact acttttccca
     2941 taagcagcta cctcttgatt tctttcattt ttgctgcata ctgttgcgcg acttccgtta
     3001 ctttgtcaaa atctcctgta gctgcgggtg ctaaaaggtt cccacctact ccgactgtta
     3061 cgacaccagc atcgaaccac tctttcatat tgtccagact cacaccgcca gttggcatga
     3121 tattgacttg aggcatcggc gccttgaagg cagaaatgat acttggtcca taagcgcttc
     3181 caggaaatag tttgacgata tctaccccgc ttttcagcgc tgttttgatc tcggtaatag
     3241 tcatacatcc tggtaagtaa ggaatttgat acagattgca catctctgcc gtttcttgat
     3301 caaaggaagg gctaacaata tactctgcgc cagctaagat agccaagcga gctgtgacag
     3361 cgtctaatac tgtgcctgcg ccaatcacta tttctggttg gtctttgtag aatgacaaaa
     3421 gttctttgat tacttgatct gcttgaggga ctgtaaaagt caactcaata ccagttagac
     3481 cacctttgat gattgcgtgg caagcattca aagcttcttc tttgcttttt ccgcgtacca
     3541 ccgcaatcac tccggcgttt ttcaatcgag ataaaatatc aatttttttc atcttctcca
     3601 cctatttcct atttagaaat tttattttat aacttggaaa tctataccca tattatacgg
     3661 cagaaaagaa aaattgcaaa cgcttttttc attatttttg aattctttgc tcgatttcgc
     3721 tttttgtttg cttgagggca ctgattattt gttcttttgt ctcatctgtt aaacgatatt
     3781 tcggcatact tacactaaat gcaccaatga tttgttcgtt tttcacgatt gaggctccta
     3841 tacaaaaaat atcacgttcg atttcttcat cgtcaaatgc gatgtgggtt tcttttaccg
     3901 ttttgatttc ctttttcaac cgtaatgcat tggtgatcgt atattctgtg taagggatca
     3961 acggatgctt ttcaagataa tcagtgtact gctcttcact gaattctgct aatacggctt
     4021 tgcccatcgc agaattgtat aatggtcggg tgattccgat ttttgaagac atgcggatcg
     4081 tttgatgttt tggatctagt ttgttcacgt acaagatctc attgttgtcc aataccccta
     4141 agtggatagt ctcatcgatc ttttgctgca gctgttctaa aaacggtaaa gtcaactcta
     4201 ccaaatcgat ttgttcaatg tttttattcg cgtagcgaat aagctttgca cctaaacgat
     4261 agtttttctc tgaattttta tttacgtagc cgatcatcaa caaagtatct agtattttca
     4321 agactgtaga agaggtcatt cccacaccct ttgcgatcgt ctgcaaagag acatcttggt
     4381 tttccgctaa atagtctagt attttagagg ctttgatcaa tacagttcca taaggctttt
     4441 tattttccat ataatattct cctatttctt tataagaaaa attatagcgg aaataccatc
     4501 aatttaaaag agatatccag atataaacta ccatttctct ttacaggcaa tttaatgttg
     4561 aagatccgct tcgtttttac atcaaacttt gatataaagc aatgatttct tctactgtcg
     4621 tttctcttgg atttcctggt gtgcatacat ctcgcaaagc atcttctgca attgctggaa
     4681 tatctgcttc ttttacacct aattcagaaa tggtttctgg gattccaacc gctttgctca
     4741 accgatcgat ctcaccacaa gccgcttctc ttacatcttc taaagacatt tcagctgctc
     4801 ctttaatccc tagaacaaga gcgatctcac ggtacttttc tccagtatat tctttgttat
     4861 atttcatgac agttggcaaa agtgctgcac acgctacgcc atgaggaatg ttgtaccatg
     4921 cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc gacatttgag aagcccattc
     4981 ccgcaatata ctgtcctaat gccatttttt ctcgcccatt ttgatcgcca tctacagaag
     5041 cttccaaaga atggccgatg atttcgattg cttttaaatg cagcatatca gtcatttccc
     5101 aagcgccttt tgtgataaac ccttcaatag catgcgtcat agcatccata cctgttgatg
     5161 cagccaattt tttcggcatt cccatcatca tatcgctgtc gatgaacgcg acgattggga
     5221 tatcatgagg atcaacacaa acaaattttc ggtgatttac ttcatctgtg atgacgtagt
     5281 tgatcgttac ttctgctgct gttccagaag tcgttggtat agctaatata ggtaaacatg
     5341 gatttttcgt atcagcaaca ccttctaaac taatgacgtc tgaaaactct ggattcgtca
     5401 aaataatccc aatggctttt gccgtatcga ttggcgaacc tccaccgatt gcgatcaaac
     5461 agtctgcctc tgcctcttta acaaaagcca ctccatcttt tacattttgg atcgttggat
     5521 taggtacgat cccgtcgtat aaagcataag cgattgctgc tttatctaga agttctgtta
     5581 cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt aatgacagct gctttgccga
     5641 atcctcgctg ctgaaattca gggacgatcg actcaatcgc tccttttcca aaatacgatg
     5701 tttcatttaa aatcattcga ttactcattt tctattcctc ctaatttgta ttatttttca
     5761 caataaaaac gaaagaaccc caagcaaccg gttgcccagt tgcttgttgc tacagttcaa
     5821 agactggcat caattcccaa ctgactggag aggcatctgg atttgtctcc atgatatctt
     5881 ccatgtactt ccaccatttt tgattgatag atgtatttgc cattttttcc cataactttt
     5941 catcttcgat cactaagtaa ccaaataaca aacttgtact cggatcaaga tagatcgaat
     6001 agtcgatgac cccgtgttca cgaagtgctg tacgcatttc tggccaaagt tcatcatgtc
     6061 tttttttgta ttcttcttgt ttttctgggt aaactttcat gcaaaaagct tttttcacca
     6121 tgtcagacac ctgctttcgt atgaacttcc tccaaataac ctgcttttgg agtgacacca
     6181 aacgcatctg ctaaatgcca caggttttcg tccgtgattg tttgcaagat tggtccttgc
     6241 gctttgacgt acgtatagac ttctgctgcc ttttctgctg tctcgatcaa accaaagact
     6301 tcatccatgt cttttcctga cccgtagatt ccatgttgtg gccataaaac caatcgagtt
     6361 tctttcattt tttcagctgt tgcttcccca atctcatttg ttcccggaac catccacgga
     6421 atgatggaaa ttccttctgg aaaaacaact aagcattccg tacacatttg ccatagcgta
     6481 cgactaaagg aacgttcgtc taattcatgc gtaaaactca tcgctaatag atgactagca
     6541 tggcaatgca taacgatccg attatctggg tctacttcta aacgggcgat atgactcatg
     6601 aaatgacttg gcaactcgct tgtaggaagg cattcgtttt ccagccccca caaaagttcc
     6661 agtgttttgc catcttcagc aactcgaacg attccaagat tctcggcagg ttcgctcgct
     6721 acatttttaa aatatcttcc tgaaccagtt acaataaaat agcatcctgc taaacggctg
     6781 gcatcaaaaa tcatcgagat gcggcggatc actttttcag gatccaagaa cggtgtgatc
     6841 tcttcttctt ttagtaaata agaaatattc ccgccatttc tttcatccca tcccaagcga
     6901 taaagattcg acgtcgtttc gatcatttct ttcacataag gcgcttgtaa aatatctatt
     6961 tttttcaata tagccacact cctattttct taattccaac acgtcttttt gatattcctg
     7021 gacttcttcc agccaatcca aaccaactgg aacttgattt ttgtaacaga aataattcca
     7081 tacatcggca tacgggaaat ctttcaattc ttccgtataa gccattcttg aagtaaagtc
     7141 aaaagccagc tccatttctt tcaatcgctc aactggttcc agcatagctt tcagcagtgc
     7201 tttttgtgta tttctcgtac cgatgaccca agcagcgata cggttgatcg ttgcatcaaa
     7261 gaaatccaga ccgatatttg ttttttccaa caattcgtta cggactaatt ctttagcgat
     7321 ttcctgcaat tcatcatcca tcaaaaccac atggtctgaa tcccaacgca ctggacggga
     7381 aacatgcaac atgattcctt tgctgaataa agctaatgaa gataatttat tggaaatcac
     7441 ttctgttgga tgaaaatgtc cagcatctag acaaacaagt ttgtcacgag tcaacccgta
     7501 acccatataa aattcatggg aaccgactgt ataagcttca gcgcctaaac caaatagttt
     7561 gctttctact gcatcttgta cataccgttc atctaactct tctgtaaaaa tttcatcgag
     7621 tgattccatt aaacgttccc gtggggtaaa acgatcaatc ggattatctt tcatcccatc
     7681 aggtacccaa aagttattga tacttgtttg cccagtttct ttcccaaagt actccgcgat
     7741 tttgcggctt cttttcccgt gttcgatcca gtattcacga acttctttat ctggatgagc
     7801 caatgtaaag ccgtctttca tcattggatg cgaaaagaaa gtcggattga agtcaagacc
     7861 gatttcttct ttttttgccc actctaccca tttttcaaaa tgtttcggct caatttcatt
     7921 tagatcgaca gattcttctg tatccaaata gatagcatga aggttcaatt tgtgtttgcc
     7981 cggtatcaac gaataagcct tctccagatc ttgtctaagc tcttcaggtg ttcgtgcggc
     8041 tcctggaaaa ttccctgtcg ccatgattcc accggtcaat tcgccatccg gtgtcaagaa
     8101 acctttgaca tcatctcctt gccagcaatg catagaaatt ttgatgtcct gtatcttttc
     8161 tagtacagca tctgtatcta caccgatagt tgcatatttt tcttttgctt gttcgtaatt
     8221 tctttcaatc gtgttcatac gattactcct ttgattttat tcacgtacca ttccatcgtg
     8281 ttttgcttat tttcatctgg gtaaaaagtt tcacatggaa acgactgttt gattacattt
     8341 cttgcttgtt ctatattcga aaactgacct gtactgagca tttgcatggc aacattcccg
     8401 attgctgtgg cttcactagg tcctgcttct acaatgattc cggcataatc tgctgtcaat
     8461 tgattcagta gacgattatt cgatccgccc cctacgatgt gcagcttttc caatttcgtt
     8521 ccagttaagt gttctacttt gatcagctct tgtgcgtaac aaagagcaag attatcatac
     8581 acacaacgag cgatagctcc cgatgagaca gggactggct gctctgtttc aaaacagtat
     8641 tgctgaatct cttctatcat atttggggga ttcaaaaatc gagggtcatt gatttccatc
     8701 acttgctgca gcggtttttc ttttgcagca agctctgcca attccgcata actgtactcg
     8761 tactcttgca ttcgggcaac ttcctggatc aaccacatcc ccatgatgtt tttcaaaaaa
     8821 cgcaccgtgt tatgggcacc ccattcattc gtataatttt cagaaaaagc attgcgagaa
     8881 acattggcga tctttgtttc aatccctaaa agcgaccatg tcccactgct gatataaccc
     8941 caacgttctc cttgacctgg cgttcctaat acagctgacg ccgtatcatg acttgctact
     9001 gtgataaagg tcacatctgg caaatcatat tttgaaaatt gttctttttg cagatctcct
     9061 agtatcgttc ccggctctgt caatggtgga aataattcct tcgagacacc gattttttca
     9121 agaagttcta tttcccattc ttttgtccct gcattcagca gctgcatagt agatgcatta
     9181 gttttctccg tcaccatgtt accagtaaag acatacccta aataatcagg tatcagtaac
     9241 aatttatgtg cttttgccaa attttctttt tcttcagcaa acaattggaa caaggtattg
     9301 aaaggctgga tctgaatccc tgtctgctca tacaacttat ctagagaata gatttcagaa
     9361 aactgttcta ctgctccatt ggtacgcttg tcccgataag caatcggatc atttatccga
     9421 tccccttgtt catcaatcag acagtaatcg actgcccatg tatcgatccc cgcaaaaagg
     9481 cgatcaatcc ctgtctgctt taatttttca aatccaatca aaatctcttt gataagataa
     9541 tcgatcttcc agcgatcgtg accgtctttg ttccgaaagc cgtttttgaa ccggtgtact
     9601 tcttccaaga tcaaagaacc ttttgcatcc ctcgtacttt tcattagtcg tccgcttgat
     9661 gcaccgatat cgatggcaac aaatgttgga ttcatttctc cacctctttt actttattgt
     9721 tgattagcac tttgttaata aagcgttatc atttattgac aatgctatca taatacagaa
     9781 tcttatattt tgcttcacac tattttggta tataattgca ttatcttgac aagttgggag
     9841 tgtttacatg gattactggg aaaaagccaa ccactcatgg tcggaagact caaaaagagt
     9901 gatcttgacg cctacacaaa gaagcaggaa tttatttttt tatatccaag aaatcggtta
     9961 cttcaaagca ccaatccctt actatactga acgagcacat ttaccttctt acttgataaa
    10021 gtttactttg tctggcaaag gttcgcttac atataatgat aatcagcaac agctaaaagc
    10081 tggagactta ttttttattg actgccaaga atatcagcgt tatcaaacga tcagcgaaga
    10141 accatgggaa atggattgga tccatttgaa tggtcagaac gtccaatcat tttatcagga
    10201 gttcacaaaa gacggccata acaccttcca ttcaacagga gatccatcag aaaacaagat
    10261 ccaccatttg atcacagaat tgatccacat gcaacaaggc acgaatgccc gtaccgattt
    10321 ccagtcctct gtagctatcc acgagttgct gaatgaactg attttgcaaa agtatcagct
    10381 agatttcgaa gaagaggata ttcccgccta cgtttacaaa ctgaagcaat acttagacga
    10441 acatttcaaa gaaacgatta cacttgatca attagaacaa caattcttat tgaataaata
    10501 tcagctgagc aaagattttt ccaaatttat cggttctcca ccaatcgatt atttgatcag
    10561 caaaaaactt tcttacgcta aagaccttct tcggtataca gaatttacgg ttcgaatgat
    10621 ttccgaagaa atagggattg agaattttgc ttactttagc cgcctgttca aagcaaaaac
    10681 aggactatcg ccgagagact ttcgtaaatt cagctgattc atataatatg tacgtcattt
    10741 tttcaaaata ttttgaaata tatagataaa aataaaataa ccgcagtttc tgcttctatg
    10801 taaacaacag cagctactgc ggttattttt tagtgatcat tctcattgtt catcttcaaa
    10861 atgttcataa tgaaccaact gtttctgtgt atcacgtttc aaaaattctt taggcgttat
    10921 gccgaactct ctcttgaatg ctttcagaaa atgcggataa gaagaaaaac cacttttact
    10981 atatacagca ctagagctgc ggtattctcg caacaagtgc ttggcataga tcagcttttt
    11041 cttcattaca tactgatgaa acgtaaatcc agtgtacttt ttaaattctc gtgtgatatg
    11101 gtatttgctg acaaaaaact gcttttccat ttcctctaaa gaaagcggat gagataaatt
    11161 ttttgagata tattgtaaca tttgttccac acgctcatta ggaactacag cattctgttc
    11221 ttgcgcactc tcttcttttt gtacaacttg attgagaaaa atcaagtatt ctaccagagc
    11281 ttgttcatat tttatgtctt caccgtaaat ttttttatca ggaggatggt cgataaatga
    11341 taatttttgc ttcaatactt cctgttctat ttgcaataca cgactcctct tctctcccat
    11401 tggttgaaaa caagccgata gattagacca tggcgtggaa cgactttcta gaaaagaagg
    11461 ggtgacaaag atatagacac gctcgaatac atctgaagat tggcggacga tacgatgcaa
    11521 atcattggaa tggatgagca gcacacttcc tgctttgatc gtaaattgtc gtccgttcaa
    11581 ataaaaagtc gcttctccct ttaatgtagc atggatctca tagaaatcat gatgatgata
    11641 gatcgtttta ttttggctga cgacatcttt gacatgatag atttcatact gatcagaaac
    11701 catcgtattg acttgttcac cgctgatttt cttttggagc atgtgccgca cctccttttg
    11761 ttcacttatg cacaatttta ttataccacg ttttttgtaa tcaaaaagag ctgcttttat
    11821 atactcttat aaaaaagaag agacgtggca tcttctagcc ccgccccctc cattctaaat
    11881 attagttaac caacgtcttc tttacattat tcaagaagac ttcttccata acgaatccca
    11941 tcatcttttg tttcttcaag aaccacatac aggtacggac gattcttcat gatgatttct
    12001 ttgatttttg tgtactcgat cacaccgtct cccaaattta ccggcacgat tttttcctgt
    12061 tcgacacgaa aatctttcaa atgaaccgca catatctttt caccaaaacg ttcaaaagct
    12121 tcttctacca attggacttg ttcttgataa tcactacttg tgatcaaatt ggtcggatct
    12181 aaaatgattc ctagatagtc cgaatctact gcttggatca gctgctctac ccttgctaaa
    12241 gaatacaccg gatgattcaa acctggttct atccccacca tcatttggta tttttctcca
    12301 gcttttacca agcgacggat aacggacaca gcttcagcaa atgcttcatc tgtgaaattt
    12361 tcttccgtat attggatttc cggcaaaaca catccagttt ccgaagcaac catcgacgca
    12421 ccaaaggaag aagcatagcg aagatactgc tcgaactttg tcagcaactc ttgtcgtatc
    12481 gttaaatctg gatgaatcat attgatatag caacttaaaa ttcctacggt cacttcttgc
    12541 ttttctaaaa ctcgtttgac ataatttccc atcccagaat tgatctcatt tgcgccagaa
    12601 ggtatttcag gaaacgaaag acctaaggcg agctgcagtg tatgaatccc ttgttctttt
    12661 gttttttctg caagatcttc tatcgtttgg acttcggaaa gatcatgtcc tctaattgta
    12721 atgttcaaca taactattct cctaactcag catattgggt agccacatga cgatttgcgg
    12781 gaagtaagta atcaaaaaca agacgactaa gataacccca taaaaaggaa gaagcggttt
    12841 cagcattgcc tctgctttta cgcctcctac acttgcaccg acatatagtc ctgtacctac
    12901 tggcggcgtg atggagccta cacacagatt catgattgaa aacaacccgt aatgtacagg
    12961 atcaacccct acacttgtga cgattggtaa aaatattggt gtaaaaatca agatagcagg
    13021 cgccacatcc ataaacatac ccaccagtaa taacacaaga ttgactaata acagaataat
    13081 aaatttatta tccgttacgc ccataatcaa cgaactaatt gcctgaggaa taccggtaaa
    13141 cgccatggcg aaagacatca tggaagaagt agccaataag aacatgatcg tccctgacat
    13201 tgctactgat tgaaacaaca tattaggtat atcttttagt tttaccgttt tatagtaaaa
    13261 catagcaatc aacagggagt aaatgacagc tatcgctgaa gcttctactg ctgtaaatat
    13321 ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa tcgcctcttt
    13381 tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt tattcttttt
    13441 agcaacgatg aatgctacta gcatgattcc tagcgcccac aaagcgccta cgagataacc
    13501 tcccataaac aaagcagaga tcgaagcacc accagcaaca agtgaataca tgataaaagc
    13561 agtacttgga ggaatgacca tacctgtcgg agctgacgcg atattgaccg ctgtcgcaaa
    13621 cttccggtca tatccttctt ccacttgctg agggatcaac actccgccaa tcgcggtaga
    13681 agcggcaatg gccgagctag agatggaacc aaataaagca ttccctaaca cattcgtatg
    13741 agccaaagcc cccggcacgc gcccgacgaa caacatcgca aaatccacta gtttcttagc
    13801 tatccctcca ttattcatga taatgcccga gagaacgaaa aacggaatag cgatcagcga
    13861 aaaactattg atacttccca ccattttttg agctgaacta aaagcagaaa catcaaacgg
    13921 tataactaac aataaagtca tcatcgatga gaatgcaata cttatagcaa tcggcatccc
    13981 taatacaagc aacacaacaa atacaaaaaa taacactaat ccggcttcta ttgccatttt
    14041 attcctccat ttttatattt tttcttgaat tgctggcttg ttgcttaatg tatcgattag
    14101 attgatcagg ctatagataa tgatgagtac ccccgataca ggtactgaca gataaacata
    14161 tcccatagga atacttaaag aaggcgagat ctgccccatt gtcaatgtga cagcattcca
    14221 tccgccatac accatgatca tcgcagcaaa taccagaaat aaactttgaa caacaacttc
    14281 taaaatttta ctttttttgc cagttaattt ttcacttaaa accgttacgg ctaaatgtgc
    14341 tttttgtccc acaacatacg cggaagacaa catggcaaac cagacaagac cgaatcgaac
    14401 aagctcttcc gtgaccgtat ttggatttcc taaaaccgtt ctggaaaata cttgccataa
    14461 aacaacagcg accatcccaa caagcaaaac agctcccagg atctctaata ccttgtttaa
    14521 aatcattttc atcacagcta cgctccttgc tcttggatct tttgataaag gtctttgtag
    14581 gtaggatcgt ttttgaatat atcatgaagt ggggcaactg cctctcgaaa cggtgtatta
    14641 tctacatcat tgaattggac tccatgttct tcttgtgcta aagctttttc ttcttcaatt
    14701 gcttttgcaa aagcttcaat ttcaaaaacc gttgattctt tcgctgcttc ttcaatggct
    14761 ttttcctgct catcagttag ctgttctttg atggcatcat tcatgatgat gatatctggc
    14821 acacgtgtat gctcatcata ggaatagtat ttagccacac caccatgtcc agctgtatac
    14881 aagacaaatt cattgttttc tgatccgttg atcaaattgg attgaagaga cgtgtacact
    14941 tcatcacttc ccataggtac gggcgaaccg cctaaaagct ctaccatttt gatagcggtt
    15001 tcgctttgca tcacacggat cttctttccc ttcaaatctt ccggcgtatg gatcggaccg
    15061 tctaccatat aaaagctgcg ctgaccagaa tcataatagg tcaatccggt aaatcctaaa
    15121 tttttggtcg attggtagat tggctccatt atttcttcat tatccatcac cttaaagaaa
    15181 tgttcttcat cattgaatag ataaggaact gaaaaaatcg aataatcttt agagaaactt
    15241 tccaacgctg aggcacttac ttttgtgaaa tctactgctc ctgactgtgt caattcgatc
    15301 agttcacgtt ctccacccag ttgtccatct gggaaatact ggatctgtac ggaaccatct
    15361 gttttttctt ttactaaacg cccaaattca ttcattgcat caattactgg ctggctgttg
    15421 cttgcataag caaagcgcaa ggtaactatc tcatttacat ctgcatctgc gacagttgcc
    15481 acactagaat tatttccgca gccgcttaga cctagcagac ctaggctaag cgaacttgct
    15541 gcaaacagca gtgatgtttt tttcatatgg gtttcctttc ttttaacaat aagagacgag
    15601 caattctgtc aaacaaagca ccgttaacga ctgtccataa ggcattgctg tgattccgat
    15661 attccgataa aaatctaaac tgtcccccat tcctgtcccg attgaaacgt tttgaacttc
    15721 tcctttttca tcgatttgtt caagcaatcc ttggatagca cgataagcga cttctttata
    15781 ttcttgtggt aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa atcctgcagt
    15841 tgcagaagat tctacgtaag aagaaggatc atccaacaac gtatgccata aaccgctttc
    15901 agactgataa gtttttaatg cacgcacttg tgcttcaagt gtttcgatca ataatttgcg
    15961 caatgcatct tctttcgata attctaagat ttcaatgatc tctggaatag caatcgtcaa
    16021 ccagcagttt ccacgagccc aaaatgcttc ggcatagtta tgatttcctt caaacgtcca
    16081 gccgtgatac cacaatcctg ttcgtttatc catcaagtat ttgatatgga tcataaactg
    16141 gtaacgagct tcttctaaat attctgggcg attcaacagt ttcccgattt ttgccaaagg
    16201 aagcactgtc atcatcaatg tatcgtccca caactgattc ttattttccg gtccatatgt
    16261 ggcatgctgt aacccgtctt catttgtccg aggcatgtca ttcatgaccc attctgccca
    16321 ttgttctaaa taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt aagccatagt
    16381 caatagcggt gccattgtat tgacgttttt gggcggagcg ccttcttcca tacgatcctc
    16441 aaaccattca ttgaccactt gataagcgcg aggatttttt gtcatctggt aatttttata
    16501 aataccatat agaccgacac cttgtggcca attccacact gtccagcttt tgtcatctac
    16561 tttcagtcca tcaaagtcca acaaaaattc accattatcg tctttgatcg ttgtcaggtt
    16621 ttcaacgagc aggtcaattt tttctttcac taattcttta ggtacaaaaa tctcttcttt
    16681 aataatcatc tttacgccac ctttttaaac gctttcattt tatatcctta atatagcagt
    16741 aataccgtcc actataaata tcgctttttg aacaaaaact gtctaaaatt gacctaaatg
    16801 ttaaataaca aaagtaactt tcagttactt ggaaattaaa aaaagaactt ggagaaacaa
    16861 cgttttccaa gttctttttc atctattttc ttatcttttt tctacgcttt cttttgctta
    16921 tccgttgtaa gcatcaatga tgatctgttt caattcagag atcaatggtt ctttcggatt
    16981 agctgtcgta cattggtctt cgtaagctaa ttcagccata cggtctacgg ttgtatctag
    17041 tgtttcttgt gtgactcctt gagcttttag actcatgttg atgccgacag attttcctaa
    17101 ttcataaaca gccgttgcta aagcttcgac taattcctct gttgtttcgc ctttcagacc
    17161 taagaatttt gcgatatctg cgtaatctgt atctgcacgg aaatagtcat atttagggaa
    17221 catcgcatgt ttttgtggat cttttgcatt atagcggata atatgcggta aaagaatcgc
    17281 atttgtccgt ccatgaggga tcccgtattc cccgccgatt ttatgtgcga ctgagtgaca
    17341 aatacctagg aatgcgttag caaacgccat accagccatt gtagaagcgt tgtgcatttt
    17401 ttcacgtgat tccatatcag gtgtttttac tgatttttct aaatagtcaa agactaactt
    17461 gattgcttgt aggctcaatc cacgtgtata atctgaagcc atgactgaaa cataggattc
    17521 gattgcatgt gttaacacat ccatcccggt atctgctgtg acagatgctg gaactgacat
    17581 gacgaattga ggatcgacga ttgcgacatc tggcgtcaat gcataatctg ctaacggata
    17641 tttcacatgt gtttcactgt cagtgatgac cgcaaatggc gttacttcag aaccagtccc
    17701 tgatgttgtc gggatacata cgaattgtgt tttttctggt ttgtcgatct tatatgtccg
    17761 tttacggata tccaagaatt tttgttttgc accaaagaaa gatgtgtctg ggtgttcata
    17821 gaacatccac attcccttag ctgcgtccat tgcagaaccg cctccaagag cgattactgt
    17881 atctggcttg aagtctgcca tcagtttcgt tcctgcgtat acggtatttg tcgatgggtt
    17941 cggttcaaca tctgagaaga cttcgatctt cacatcattt ttacgtcgtg ccaatacttc
    18001 acgaactgta tcgcagtaac cgaattggac cataccaggg tcacaaacga tcatgacacg
    18061 ttcaacgttt tccattttct ctaagtacaa tagagagttc ttttcaaaga agatttttgg
    18121 cggtaatttg aaccattgca tgttatttct ccgtttcgct acagttttga cattgattaa
    18181 gtttacagca gagacgttac gagaaacaga gttcttacca taagaaccgc atcccaaagt
    18241 caatgacgga atcatttcgt tatagatatc accgatgccg ccttctgcag atggtgtatt
    18301 cactaaaata cgacaagctt tcattcgaag accgaatttg acatgcaact cttcgttttc
    18361 tgaatggata actgctgtat gtcctaatcc tccaagattc aacatacctt cacacaaatc
    18421 aaacgcatgg tccgtgttgt tagctttgat catggcaaga actggagaaa gtttttctct
    18481 tgatagcgga taatctgggc ctacgccttc caattcagca atcagcattt tcgttccttt
    18541 tggtacactg attcctgcca attttgcgat ttccatcgca gaatgtccaa cgattgatgg
    18601 attgaccgca tattttcctt cgttcatcac tgcatcttct aatttttgca attcatctgg
    18661 ttttacgata taaacttggt gtgcttggaa ttctgctttt actgctgcat agatttcttt
    18721 atccacgatc acagcttgtt cagaagcaca aatcattcca ttatcaaatg ttttcgaaac
    18781 gatcaaatcg ttcactgcac gtttaatttt tgctgttttt tcaatataag ctggtacgtt
    18841 acctggacct acacctaaag ctggttttcc tgtagagtaa gcagacttga ccattccagc
    18901 tccacctgtc gctaagacga tcgcgatacc tggatggttc attagcatag aagtcgcttc
    18961 gatcgaagga tgttcgatcc attggataca attttcagga gctccggcag cgatagcagc
    19021 atcacgtacc actcgtgctg cttctgcaga agatttttgg gcacttggat ggaaagcaaa
    19081 aacgattgga tttcttgttt tcaacgcgat cattgatttg aaaatcgttg tggaagtcgg
    19141 attcgttgtt ggagttactc cgcaaaccac accgactggt tcagcaattt caatcaaccc
    19201 tttttggaca tcttcattga tcacaccaac tgttttatca tgtttgatat tatgccaaat
    19261 atattcggac gcatacatat ttttgattgc tttatcttcg taaacaccgc ggcctgtttc
    19321 ctctacagcc atcttcgcta atggcatatg ttgatccaaa gctgccattg ccatttcatg
    19381 cacgatatgg tctacttttt cttgatcaaa gctttccatt tctttcaatg ctgcatttgc
    19441 tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt tttctttttc
    19501 tattacttta gccatcatta tcctcctaat gggtcatggt tatttgttaa ataattcaca
    19561 tgtatatcat acaatattta tttttctatg taaacacttt tttgtgattt ttttcacagt
    19621 tatttttaaa tataaaattg ctttatttac aggtgtttgt atagaaaaat atataaagtt
    19681 atttattatt atttatatga cacacccgat tattcaatat tgagcaatat atcccatgct
    19741 cttatctctt tcttttttga ataaaaaatg aggtaggaca tctttctgcc ctacctcgtc
    19801 tttaaataag ctttgatact tatttttctt ccttctcttc tgtctcttcc agcgtttctg
    19861 ttgtttcagt ttttacttca actgtctcag ctggttcttc tgttgtttca acagtagtcg
    19921 ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt gattgctgca cgatcgtact
    19981 caaggaagat cccttcgcag tctaaaacaa ctgtccgttt gtcattgtcg atttctgaaa
    20041 caactccatg taatccgccg atcgttacga ctgaatcacc tggtttcatg ctatctaaca
    20101 agttttgacg ttcttgctgt tgtttctttt gcgaacgtga cataaaaaac cacattgcta
    20161 atagcgcgat aaacatcaca agcattggta atgttcccat ttttttcacc tcactaattc
    20221 tgtatctaca aaaaacactt tagcagaaaa atgctgataa cactagtttt ttacttaatt
    20281 tttaaaagat tttcctttag aagctttttg cgttttcttt attgaacccg tattcttcaa
    20341 aaaaggcttc tctaaattca agcaaattat catccatgat tgcttgacgt acttgtttca
    20401 ttagattcaa caagaagtat aaattgtggt aagaagtcaa acgaatccca aatgtttcat
    20461 cacatttgat caaatggcgg atataggcac gtgtatagtt tttacatgta taacaatcac
    20521 atttctcatc aattggacgg aaatcatggg catatttcgc atttttcact actagacggc
    20581 cttgagaagt catacatgtg ccatttcgag cgattcgagt aggcaagaca cagtcaaaca
    20641 tgtctactcc acggataaca ccatcgatca acgaatcagc agcaccgaca cccatcaagt
    20701 aacgtggttt gttttcgggg atcaacggtg tagtgaattc taatacacga ttcatttctg
    20761 atttaggttc cccgacagat aacccgccga ttgagtaacc agggaaatcc atcccgacta
    20821 gatctttcgc gctttgacga cgaagatctt caaaccctgc tccttggatg attccaaata
    20881 atccttgacg gtctggatta gcatgagctt tcaatcctcg ttctgcccat cgagacgtac
    20941 gttctactga ttttttcacg tagtcgtaac tttcatcgaa tggtggacat tcgtcaaaac
    21001 tcatcatgat atcagagccc aatttatttt ggatgttgat tgctttttct ggtgataaaa
    21061 acatacgcga accgttcaag tgatttttga aatgcacgcc ttcttcttcg atattccgca
    21121 tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt taaaatcggt tgatcccagt
    21181 tcataaattt atgcagtccg ccagcttctt ctactaagtc ttcaccaggg cgtagccata
    21241 aatgatatgt attacttaaa ataacgccag cacccatttc tttcaactct tctggggaca
    21301 ttgtttttac tgtagctaat gttcctactg gcatgaacat tggtgtgggg aaagttccgt
    21361 gtggcgtgat caattcccct aaacgcgcgc cagtatgttt ttctttcttg attaaacgat
    21421 agcgaatggc aggttgcgtc attcatttcc ctactttcta tataaaatat cttgacaact
    21481 cccaccatca taatcgtttc aagtgctttt tgcaagttcc ctcggcttcc ttctacttta
    21541 tttaatattc tttttttatc ctttttttat gaataacaaa aaaaagaaaa acattatgac
    21601 agaaatattt tttgatacat ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat
    21661 gcaatcttct gcagaattaa aacgccaagc aaaagagagt ctaaaaggac gttggggaca
    21721 agctgtttta ctaaatctaa tccctacatt gattggtatt gctactattc ttcttattat
    21781 gattcctatt atcttgtttg ccaccaccat atatgatgga tcagcgagcg tctcagaagc
    21841 gacaacttca ggaggcggta gttcaggagg cggtggcgga attatttcca gcatcattag
    21901 cgctttgttc atgagcggta tttcttggac ttatcttgat attttacgtg gacaaaaaga
    21961 tcagatcgaa ccattcaaag atgcatttcg tggattcaaa ggtttgttta tcggcggagt
    22021 gatcagctta gctgtgctga tctcgatctt tacaacatta tggggtttgc tattagttat
    22081 tcctggaatc attaaaagct attcttattc tcaaagctat tttatttact acgatatcgt
    22141 ccaagaaaca ggacaaacac ctaaagtact ggatacgatc actgcaagtc gaaaattgat
    22201 ggatggttat aaaggacaac ttttctggtt agatctttca tttatcggct ggcatatctt
    22261 agcattagca actgctggta ttggttatct atggctcagc ccatacattt ctgcaacaaa
    22321 agcagcattt tatgaacaat tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga
    22381 gggggtggca ggagaacacc gtttatcgag aggcagtaaa agaaacattc agcccattag
    22441 caaaaaggag gtgtgacaac attcatgtca cacctccttt ttgtatagtt ccagtaaaaa
    22501 gaaaatcaac ttagaaacag ttaatcattc ctcttcttct gcttccggta atcgatggaa
    22561 cttgatcagt tcattcaccg cgattggtac gatagataaa atccctacca agaaccagtg
    22621 attcaaactg atgtgtacta ggccgaatag ttcttgcgta aatggcagca aagcaacagc
    22681 cgttgtaata gctaatgcca gtaacgccat ttctaccaaa gctttattgg tcgctaaatt
    22741 gactttgaag atcgattggc tgctgcgtac attgaacaca tgcagaatcg atgaataagc
    22801 taagatcaag aatgcaacag tttgaccgac atgctgactt gcgtctaccc atgggctgat
    22861 agtatcgaca taactaccta aataataacc aaacaaggtt acgatggtga agacccctgc
    22921 atttatggca attttttgca ataaacctcg agcaaagatc ccttcatttt tcggaatggg
    22981 tggctgatcc atgatgccgt gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc
    23041 atcagcaact acattgacaa acagtagttg cacagctgta aatggtgctc cccatcctaa
    23101 cagcatcgca atcaaaacaa taaagatctc cgaaatatta cagcttagca aaaagttgat
    23161 agctttacga atattctgat aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc
    23221 aaagttgtca tccgtcaaaa tcatatctgc ggcactttgc gccacatctg ttcctgtgat
    23281 ccccatggca cacccaacgt tacttgcttt caacgctggt gcatcattca caccatcccc
    23341 agtcatcgct acgactgcac ccgtacgttg ccaagatttg acgatccgga tcttgtcttc
    23401 tggcgttacc cgagcataga cagagagagc tttgacacgc gcatctaatt cttcgtctga
    23461 taattgatgc agttctgatc ctgaaagtgc ttctgacgga tcattcaaga tacctaattc
    23521 tttcgctatg gcactggcag tcaccacatg atctccagtg atcatgactg ttttgatgcc
    23581 agcctttttc gctcgcttga ttgctccttt actttctgga cgtggtggat cgatcattcc
    23641 gatcagacca atcaaacgta agtctttttc cagtgcttca gaagaaatat cttgcggttc
    23701 ttcgtcataa gtagcataac caacagcaat cactctcagt gcccgtttac caaagcgatc
    23761 attaacgatc gccgcttgtt ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa
    23821 tgctcctttg gtgattgaga tgtatttttc gcccatttgg tgaactgttg tcatcatttt
    23881 tcgttcagaa tcaaaaggaa gctcgtttac tcgtggatat ttttcttcta attctgcctt
    23941 tgtatgataa ttctcttcaa cagcccttac gatggctact tctgttggat tacctttgat
    24001 aacaaggtcg tcatcttctt gctcgaccgt cacatctgta catagtgcag ccattttcaa
    24061 tatttccatc gcgctgttcg tcattgaatc ttcgatattc gttacttcat cttctttgct
    24121 ccatacccgg cggacgcgca tttcattttg tgtcagcgtt cctgttttgt ctgagcagat
    24181 aacattagcg gttcctagcg tttcaactgc tggcaactgt cggatgatcg catgtttcct
    24241 cgccatcttc tgcacaccgt atgcaagtgt caacgtaaca ataacagtca aggtttccgg
    24301 tacagctgca acagccaagg aaatcgaagt catgaacatc tcaaggagtg gttctccttg
    24361 cagttcacca atcacaaata ctaaagccgc agccgctaaa gcgatcatac tgattcgttt
    24421 gcctagctga ttcaatcgga cctgtaaagg tgttttttgc atagtatttt cgttcaaaag
    24481 atccgcgatc ttacccattt ctgtagccat gccaatggca gtgacgatcg ctttcccacg
    24541 acctgcagca accgtacatc ctttaaatac catattaagt tggtcaccta atgactcatc
    24601 ttcttcagca ataaatgctg catctttttc aactgcttca ctttctccag tcaacgccga
    24661 ttcttctaca cgtaattgtg atgcttcgat caaacgggca tcggcagaaa tagcatcacc
    24721 ggactctaaa acaagaatat cacccactac cagttgctct gactcaacag tggtcacttt
    24781 gccgtctcgt atgacagttg ccgtttgttt attcatatct tgcaaggatt ccagtgcttt
    24841 ttctgcgttt ccttcttgga caatcgccaa taccgagttg atcacaacga tagcgatgat
    24901 cagcagtcct tcaaataaat ccccatgttc tgtagcaaat gcggtataaa aggaaatcac
    24961 cgctgctaca agaaggatga tcgttgtaaa atcggacaag cttcgaaaga attttttgat
    25021 catcgattct ttcggcgcct catcgaattt attcaatccc tgcttttttc gccgttcatc
    25081 cacttcttgt gaagacaacc cagtatctgg atctgcttct gttaccttct ttacttcttc
    25141 tattgtctgc ttgtattctt ccatcctatt cctcctttaa aatcttcgta caaaaaaagg
    25201 tacttatttt actataacat ccttttttta tttacttcaa ataaagggct tatcgattat
    25261 ttgttaagaa agcgtaaaat ttaaagtgct tatatttgca aacgctacca aaggatgcta
    25321 ttatatagat gtaaggaagg aggataccat caaatagaca gaagaatttc tcgatagaac
    25381 agtgtaagca aacaaagttg ttagaagtac aggtttcacc cagacatgcg tgtagagtac
    25441 aaacctataa atagtgaatg cgtatggatg tttggatcac gaagtatccg aaacaagaag
    25501 taatgcgata cgtaacaatt gaataacgta aacgtctgaa aattgaatag gaacaagaga
    25561 tgtttcaaat gttgaagatg taacgaaaac aaaatttttg tttaacatga agaaattcaa
    25621 caccgtgctt tgcatgtatg atggaaagtt acaatcattt ctgtttattt gaatccttcc
    25681 gaggtacaag aaagccgagc ttatcgagag ctcggctttt tctttgtcat aaaatctttg
    25741 taaagctttt ttgttattca aaactagcgg cttttcataa ccttatttca cgaacatcgc
    25801 atctccaaaa ctaaagaaac gatagcgctc ttcaatcgca tgctggtaag cagataaagt
    25861 caattctctt cctgcaaagg cactaactaa catgactaaa gtcgattttg gcaaatggaa
    25921 attagttgag aaagcttcta ccactttgaa ttcatagcca ggcgtaatga aaatatcagt
    25981 ccatccgctg tctgctttga tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg
    26041 aattgaagtt gttccgacag ccacgatttt cccgccattt ttacgtactt ggttcaatcg
    26101 ctcagccgct tcttccgtca aacggtagaa ttcactatgc atatggtgtt cttcaatttt
    26161 atctacgctg acaggtcgga atgtacctaa ccctacatgt agtgtcagat agactaattc
    26221 aacacccttt gcttcgatct catcaagtaa ttctttcgta aaatgcaatc cagctgttgg
    26281 cgctgcagca gaaccatttt ctttcgcata gacagtttga tagcgatctg ggtcttccag
    26341 acgttctttg atatatggag gcaatggcat ttctcccaat gattcaagaa tctctagaaa
    26401 aattccttca tactcaaagt caatgatccg tccgccgtgt tccaattctt ctttcactac
    26461 tgcttgcaat ctgccatcac caaaagagat tcttgtccca actttggcac gtttcgcagg
    26521 tttgatcaat gtctcccacg tatctccttg tgtattcgtt agaagaagga cttctaaatg
    26581 cgcaccagta tctggttttt ctccatataa acgagcaggc agaactcgtg tattattcat
    26641 aactagcgca tcaccaggct gtaactcttc aatgatttca tggaaatgtt tatcttccat
    26701 ctctccagtt ttccgatcca aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt
    26761 ttgtgcgatt aattcttccg gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc
    26821 actcactttc tatcatgtgc ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc
    26881 agcacttttt cacttctatt ttttgttttc ctaaataaaa aaactaccct atttgtattt
    26941 tttcattgtt ttaggcatac tagtaataga tagttcttca ttgaaaatag aaaaagaggt
    27001 ggcaccatga ttgaatttca aaatgtgtca aaaatttata aaggtggaaa agtcgctgta
    27061 gaggacgtca atctttcatt tgaaaagggt gagttcattt gttttatcgg tacgagcgga
    27121 agcggaaaaa cgacgacgat gcggatgatc aaccgaatga ctgatccaac acaagggaaa
    27181 attttgattg atggaaaatc catccaagaa attgatccag tggaattgcg acgtcagatc
    27241 ggctatgtta tccaaagtat tggattgatg cctcacatga cgatccgtga aaatatcact
    27301 cttgtccaaa aattgttgaa agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg
    27361 attgatcttg tggaattgcc ccgtgagatg ttagatcgct acccacatga attatctggt
    27421 ggtcagcagc agcgtattgg agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg
    27481 atggacgagc cattcggtgc gcttgatcca atcactcgtg attccttgca agaccttgtc
    27541 aaagatttac aagagcgttt gggaaaaacg atcgtatttg taacccatga catggatgaa
    27601 gcgattaaac tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat
    27661 acaccagaaa atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa
    27721 gatcgcctac tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca
    27781 gcaatcacga ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa
    27841 cgtgtagata cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta
    27901 gaaacgttga ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat
    27961 gttttttacg taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc
    28021 gggctgaaat acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt
    28081 gcatcattag tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat
    28141 gttgcagagt cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt
    28201 catcagcacg gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt
    28261 gctttgatca ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca
    28321 aaagctgcca cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct
    28381 ttattggcat tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc
    28441 tttatttatt cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat
    28501 tggaattatc gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc
    28561 gttgaattgc ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac
    28621 gtgattgcat gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt
    28681 tttagtggac tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca
    28741 attttagcac tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc
    28801 gcacttagag aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct
    28861 ctcttcttat acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca
    28921 ccatttcgat cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg
    28981 gaatggttga acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa
    29041 ctatcaacca tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag
    29101 gaacagattt aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg
    29161 ctaccgtgaa agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatagtt
    29221 ttgaaaatac gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta
    29281 aagtcagcga tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga
    29341 tcaatcgtaa aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt
    29401 cgatattacc tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata
    29461 ttgttcttgg ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag
    29521 atgatcgtca attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag
    29581 ctgatacgcc tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa
    29641 aaatgcagga attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac
    29701 aacgtttctt acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa
    29761 tgtctttcaa caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt
    29821 tatccgtcac ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc
    29881 ggttgggatc atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa
    29941 tatcatccag acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct
    30001 gggagtcaat gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa
    30061 tacgtatacc gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg
    30121 gatgactgct agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat
    30181 ggcaggcata agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt
    30241 cggtgctggt ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc
    30301 gattatttta gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt
    30361 aggtattttg gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa
    30421 aaaacaaaag cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt
    30481 taaggcaata atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc
    30541 tatttatcca ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac
    30601 cgactaagtt tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt
    30661 catggaattc ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat
    30721 caattcttac atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt
    30781 tcgatgcctt cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc
    30841 ttttatacaa tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc
    30901 agcttctcgc catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc
    30961 atccgtcgca actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa
    31021 ccaattgatc gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc
    31081 gtgatgcatt gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt
    31141 ccgtttgagt gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa
    31201 cgacactggt attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct
    31261 ctgggaacca ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg
    31321 ctgtttctcg ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg
    31381 tcgttgccac tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac
    31441 ttcttcctcc ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac
    31501 ccttcaaagg tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt
    31561 tggttttaga acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa
    31621 aggttggctc ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt
    31681 attttcctca tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct
    31741 gccttcatgt gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt
    31801 caatgtctga atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa
    31861 atccactttc gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa
    31921 tcaatatcat cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct
    31981 aaagtacctg tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca
    32041 atcaatacag cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca
    32101 tttacctctt tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta
    32161 ttagccattg tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc
    32221 aaaaaaatac aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa
    32281 aagacccctt tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc
    32341 ggtagccaaa cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa
    32401 agcctgtgag tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg
    32461 attgatatct tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact
    32521 gattttggcc gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca
    32581 ttcatgttca ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta
    32641 aaaatggcac tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact
    32701 tatgaagaca catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc
    32761 tgtacattat ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt
    32821 tacagctaaa taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat
    32881 agagggcacc tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta
    32941 gagggtctgt ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct
    33001 gacaacgata ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg
    33061 gagaatattc tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata
    33121 gttttgtttc gctgcatttt tacttacgat atctacataa cggataattg gattttcaat
    33181 cgagcgataa gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc
    33241 tgcttcgatt tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac
    33301 ataatcaccg atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc
    33361 gactaataca agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg
    33421 aagctgttct gctacttttt tataatgatc atggatctta taaaaaatga acataacgat
    33481 tggcataatg atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat
    33541 gattgcatat gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt
    33601 tccttctttt ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac
    33661 gccgatagag tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac
    33721 gatcgaacct agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc
    33781 ttgatacatg tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc
    33841 agaaaaaccg gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta
    33901 atacaaaata ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt
    33961 ttcaggaacg atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa
    34021 gatcaatgcc atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa
    34081 tggtacagca ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc
    34141 tcgtaaaatc aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg
    34201 caatggcaca gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac
    34261 aaggataaat gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag
    34321 attcagcatc atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag
    34381 tgctggcacc gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa
    34441 catgtagtcg atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc
    34501 actgctgacg acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga
    34561 tagcgttaat gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat
    34621 ggctgcagca gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga
    34681 tgataacgca tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc
    34741 attttcagca gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta
    34801 aactccttta cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga
    34861 tgttatagta cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa
    34921 aacagaaaaa tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt
    34981 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    35041 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    35101 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    35161 gcttgcatct gcggtaataa gcccaaccaa attaaaaaca taagaagctg tttttatttg
    35221 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    35281 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    35341 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    35401 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    35461 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    35521 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    35581 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    35641 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    35701 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    35761 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    35821 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    35881 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    35941 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    36001 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    36061 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    36121 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    36181 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    36241 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    36301 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    36361 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    36421 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    36481 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    36541 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    36601 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    36661 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    36721 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    36781 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    36841 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    36901 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    36961 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    37021 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    37081 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    37141 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    37201 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    37261 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    37321 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    37381 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    37441 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    37501 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    37561 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    37621 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    37681 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    37741 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    37801 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    37861 tgctccccaa tgaccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    37921 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    37981 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    38041 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    38101 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    38161 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    38221 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    38281 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    38341 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    38401 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    38461 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    38521 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    38581 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    38641 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    38701 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    38761 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    38821 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    38881 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    38941 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    39001 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    39061 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    39121 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    39181 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    39241 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    39301 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    39361 ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt
    39421 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    39481 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    39541 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    39601 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    39661 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    39721 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    39781 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    39841 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
    39901 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
    39961 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
    40021 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
    40081 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
    40141 tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt
    40201 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
    40261 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
    40321 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
    40381 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
    40441 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
    40501 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
    40561 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
    40621 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
    40681 gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc
    40741 tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc
    40801 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
    40861 gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc
    40921 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
    40981 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
    41041 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
    41101 cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc
    41161 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
    41221 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
    41281 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
    41341 atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
    41401 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
    41461 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
    41521 agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt
    41581 cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
    41641 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
    41701 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
    41761 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
    41821 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
    41881 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
    41941 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
    42001 tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct
    42061 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
    42121 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
    42181 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
    42241 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt
    42301 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
    42361 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
    42421 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
    42481 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
    42541 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
    42601 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
    42661 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
    42721 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac
    42781 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
    42841 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
    42901 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
    42961 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
    43021 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
    43081 gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
    43141 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
    43201 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc
    43261 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
    43321 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
    43381 tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac
    43441 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
    43501 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
    43561 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
    43621 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
    43681 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
    43741 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
    43801 agatttttat ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct
    43861 gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt
    43921 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
    43981 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
    44041 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
    44101 taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc
    44161 catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt
    44221 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
    44281 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
    44341 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
    44401 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
    44461 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
    44521 tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
    44581 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
    44641 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt
    44701 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
    44761 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
    44821 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta
    44881 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt
    44941 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
    45001 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
    45061 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
    45121 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
    45181 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
    45241 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc
    45301 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
    45361 cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
    45421 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
    45481 tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
    45541 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga
    45601 atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct
    45661 tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg
    45721 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
    45781 gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc
    45841 atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
    45901 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
    45961 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
    46021 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
    46081 tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc
    46141 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
    46201 tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct
    46261 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
    46321 gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta
    46381 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
    46441 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga
    46501 acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
    46561 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa
    46621 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
    46681 aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc
    46741 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
    46801 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
    46861 tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg
    46921 ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc
    46981 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
    47041 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
    47101 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
    47161 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
    47221 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg
    47281 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
    47341 caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg
    47401 atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta
    47461 gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
    47521 cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
    47581 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
    47641 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
    47701 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
    47761 ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata
    47821 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
    47881 tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac
    47941 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
    48001 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
    48061 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
    48121 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
    48181 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
    48241 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
    48301 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    48361 agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    48421 ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca
    48481 cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc
    48541 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    48601 ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc
    48661 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    48721 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    48781 actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    48841 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    48901 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    48961 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    49021 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    49081 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    49141 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    49201 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    49261 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt
    49321 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    49381 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    49441 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    49501 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    49561 gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    49621 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    49681 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    49741 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    49801 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    49861 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    49921 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    49981 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    50041 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    50101 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    50161 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    50221 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    50281 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    50341 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    50401 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    50461 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat
    50521 aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca
    50581 aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg
    50641 tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa
    50701 tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat
    50761 ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt
    50821 ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcttt
    50881 tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg
    50941 tactttattt agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc
    51001 agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag
    51061 ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg
    51121 accaatgatc ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc
    51181 cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt tacattataa
    51241 atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca
    51301 cggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc
    51361 tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt
    51421 tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga
    51481 taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc
    51541 cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat
    51601 gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa
    51661 cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt
    51721 gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag
    51781 accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta
    51841 tattccccac atcttttttc atttattaaa actgcctcct t
//
LOCUS       DANJMA010000014        51625 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000014 DANJMA010000000
VERSION     DANJMA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            25..696
                     /locus_tag="PWU31_000989"
     CDS             25..696
                     /locus_tag="PWU31_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1083550.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="PWU31_000990"
     CDS             755..1195
                     /locus_tag="PWU31_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083551.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="PWU31_000991"
     CDS             1207..1875
                     /locus_tag="PWU31_000991"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1083552.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="PWU31_000992"
     CDS             1939..2562
                     /locus_tag="PWU31_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL1083553.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="PWU31_000993"
     CDS             2618..3004
                     /locus_tag="PWU31_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL1083554.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="PWU31_000994"
     CDS             3217..4608
                     /locus_tag="PWU31_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL1083555.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="PWU31_000995"
     CDS             5219..5587
                     /locus_tag="PWU31_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL1083556.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="PWU31_000996"
     CDS             5767..6708
                     /locus_tag="PWU31_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL1083557.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="PWU31_000997"
     CDS             6777..7508
                     /locus_tag="PWU31_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL1083558.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="PWU31_000998"
     CDS             7652..9832
                     /locus_tag="PWU31_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL1083559.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="PWU31_000999"
     CDS             9852..10334
                     /locus_tag="PWU31_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL1083560.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="PWU31_001000"
     CDS             10350..11111
                     /locus_tag="PWU31_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1083561.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="PWU31_001001"
     CDS             11242..12576
                     /locus_tag="PWU31_001001"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL1083562.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="PWU31_001002"
     CDS             12883..13368
                     /locus_tag="PWU31_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL1083563.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="PWU31_001003"
     CDS             13361..14044
                     /locus_tag="PWU31_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL1083564.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="PWU31_001004"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="PWU31_001004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL1083565.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="PWU31_001005"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="PWU31_001005"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL1083566.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="PWU31_001006"
     CDS             17660..18289
                     /locus_tag="PWU31_001006"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL1083567.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="PWU31_001007"
     CDS             18341..18673
                     /locus_tag="PWU31_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL1083568.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="PWU31_001008"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="PWU31_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL1083569.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="PWU31_001009"
     CDS             19499..20872
                     /locus_tag="PWU31_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL1083570.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="PWU31_001010"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="PWU31_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL1083571.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="PWU31_001011"
     CDS             21927..22367
                     /locus_tag="PWU31_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL1083572.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="PWU31_001012"
     CDS             22511..23212
                     /locus_tag="PWU31_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL1083573.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="PWU31_001013"
     CDS             23209..24330
                     /locus_tag="PWU31_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL1083574.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="PWU31_001014"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="PWU31_001014"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL1083575.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="PWU31_001015"
     CDS             25709..26617
                     /locus_tag="PWU31_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL1083576.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="PWU31_001016"
     CDS             26652..26924
                     /locus_tag="PWU31_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL1083577.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="PWU31_001017"
     CDS             26935..27981
                     /locus_tag="PWU31_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL1083578.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="PWU31_001018"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="PWU31_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL1083579.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="PWU31_001019"
     CDS             complement(30992..31678)
                     /locus_tag="PWU31_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083580.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="PWU31_001020"
     CDS             complement(31656..32150)
                     /locus_tag="PWU31_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL1083581.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="PWU31_001021"
     CDS             complement(32170..33390)
                     /locus_tag="PWU31_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1083582.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="PWU31_001022"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="PWU31_001022"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL1083583.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35568..36479
                     /locus_tag="PWU31_001023"
     CDS             35568..36479
                     /locus_tag="PWU31_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083584.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            37475..37726
                     /locus_tag="PWU31_001024"
     CDS             37475..37726
                     /locus_tag="PWU31_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083585.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            37809..38081
                     /locus_tag="PWU31_001025"
     CDS             37809..38081
                     /locus_tag="PWU31_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083586.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            38171..38410
                     /locus_tag="PWU31_001026"
     CDS             38171..38410
                     /locus_tag="PWU31_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083587.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            38557..42840
                     /locus_tag="PWU31_001027"
     CDS             38557..42840
                     /locus_tag="PWU31_001027"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL1083588.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPVLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            42939..43304
                     /locus_tag="PWU31_001028"
     CDS             42939..43304
                     /locus_tag="PWU31_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083589.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43306..43758
                     /locus_tag="PWU31_001029"
     CDS             43306..43758
                     /locus_tag="PWU31_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083590.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44372..45681
                     /locus_tag="PWU31_001030"
                     /pseudo
     CDS             44372..45681
                     /locus_tag="PWU31_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            45704..46015
                     /locus_tag="PWU31_001031"
     CDS             45704..46015
                     /locus_tag="PWU31_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083591.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            46376..47632
                     /locus_tag="PWU31_001032"
     CDS             46376..47632
                     /locus_tag="PWU31_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1083592.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            47629..48174
                     /locus_tag="PWU31_001033"
     CDS             47629..48174
                     /locus_tag="PWU31_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083593.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48175..48675
                     /locus_tag="PWU31_001034"
     CDS             48175..48675
                     /locus_tag="PWU31_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083594.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            48756..49312
                     /locus_tag="PWU31_001035"
                     /pseudo
     CDS             48756..49312
                     /locus_tag="PWU31_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49332..49676
                     /locus_tag="PWU31_001036"
     CDS             49332..49676
                     /locus_tag="PWU31_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083595.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            49695..50606
                     /locus_tag="PWU31_001037"
     CDS             49695..50606
                     /locus_tag="PWU31_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1083596.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            50685..>51625
                     /locus_tag="PWU31_001038"
     CDS             50685..>51625
                     /locus_tag="PWU31_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1083597.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYV"
BASE COUNT        17584 a         8599 c        11063 g        14379 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa
    35401 taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc
    35461 atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt
    35521 tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt
    35581 caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag
    35641 ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa
    35701 tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag
    35761 atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata
    35821 agttacttaa caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg
    35881 tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat
    35941 taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat
    36001 caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag
    36061 agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta
    36121 gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg
    36181 gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag
    36241 atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga
    36301 aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa
    36361 tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa
    36421 ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa
    36481 aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata
    36541 aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg
    36601 cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat
    36661 acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca
    36721 atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga
    36781 tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag
    36841 cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg
    36901 ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct
    36961 tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt
    37021 tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa
    37081 aaacggcatc gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata
    37141 aaaaatgatt ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat
    37201 cagcattcaa caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt
    37261 gttgtgaaga taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt
    37321 aggttaggac aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag
    37381 taatttccga ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc
    37441 tagatttcgc gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac
    37501 acgaggacaa agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga
    37561 ttgtttcgag gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa
    37621 caacgtggaa tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa
    37681 atttattttg cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta
    37741 gagatcattg ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag
    37801 gtgatagaat gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga
    37861 ttcttagaaa aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg
    37921 taggtgtcta tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag
    37981 atgcctattt acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt
    38041 ttgaatggga aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa
    38101 ataagccagt agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga
    38161 tcattatggt atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat
    38221 tggcaatagt gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga
    38281 aattaaggat gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa
    38341 aaaagctggt attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt
    38401 tgttctgtaa ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc
    38461 cagataaaaa tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt
    38521 tattatataa aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat
    38581 tggttgaaag cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct
    38641 caagcacatg cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc
    38701 gcttctgaag tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact
    38761 gttgaacagg cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag
    38821 acgattgggg aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa
    38881 actgcctcaa ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc
    38941 gaagcaacgc cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga
    39001 atagaagaaa cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa
    39061 aacgttgaaa aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac
    39121 aaagaatcta cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca
    39181 ggtacaccta cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca
    39241 accaatgcag ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact
    39301 tctagtccga acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga
    39361 caagtggcgt tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct
    39421 actgctttga aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct
    39481 tatttaggat atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt
    39541 atgatttggg aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat
    39601 cgtcgaaaag cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat
    39661 aatcaaacac atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt
    39721 ttgaaatatg tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat
    39781 gaagtatgga ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa
    39841 attaaagttc aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg
    39901 ttacatccaa cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac
    39961 attcgtgtgt ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta
    40021 gaaaatgcgg tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg
    40081 aaaaaaatta acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt
    40141 ttaaaagaaa tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt
    40201 gttgaacctg gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt
    40261 gatttagtga aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca
    40321 aaattagctg gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc
    40381 gttgtgacag acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg
    40441 tacattcttt ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc
    40501 acaatttctg aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa
    40561 cgtcttaaag tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca
    40621 gaattcaaga tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat
    40681 acagaaacaa cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca
    40741 cttacttatg gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt
    40801 ttgaatcaaa acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc
    40861 cattttgcga accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac
    40921 aagaagaaaa cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca
    40981 attggtgaat ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat
    41041 gactattact tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa
    41101 gcattcacgg ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct
    41161 gtacgtaatt acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt
    41221 gaaaaagtgg ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac
    41281 ttgtttgtcg gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg
    41341 ccaattttac aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc
    41401 aatggcacaa aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc
    41461 gttgtttttg aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac
    41521 gatgaagccc aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa
    41581 gacggattga cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt
    41641 accgagattc ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc
    41701 ggtgaaccct tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa
    41761 gcagaaaatg aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt
    41821 gaaatcgtcg tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac
    41881 atcaacgaca aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca
    41941 cataaaccca ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct
    42001 gtgaaatacc acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac
    42061 aaagtaacgg aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt
    42121 gtggcagaaa caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg
    42181 cgaggcaaag aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct
    42241 cacgcagata ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg
    42301 aaagcgacga ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt
    42361 gaatgggtca aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc
    42421 tacttaacac cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc
    42481 gataaagaaa tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga
    42541 aaaggcttga gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt
    42601 gaagaagtgg cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg
    42661 gaagcgccaa aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt
    42721 gtaccagtgt tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta
    42781 gtagtattag tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag
    42841 tcatgaggaa caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt
    42901 tagaatttat tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta
    42961 tgaagaaaga gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa
    43021 aggaaattaa tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag
    43081 aacagcctgc tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag
    43141 aaaaagaggt gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga
    43201 atagtcgtga aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg
    43261 ttggtgtctt tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt
    43321 ccaaataatt atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt
    43381 ggcgcattag aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa
    43441 attcgatcac agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga
    43501 cgagttaaat ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa
    43561 ttaattgatc cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat
    43621 tcagatttag tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct
    43681 aatatgaaac aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag
    43741 gaaaaagaga aaaaataagg tttttttgaa agaataggaa ggagtcttcc tattcttttt
    43801 atctaagata gaaggaaggg gagattttga attcagataa aaccaatcaa atactttgag
    43861 agattgtgct tgttatcaaa atatccaaca attgattgag tattttggag acaatccaac
    43921 gatttttgta caaacaaatg catatatttg tgcggttatt gggcagtgta tgtttttacg
    43981 tgatcttgtt cgtttttcat taccgtacca gtggaaacaa gatcatcagg aagtgatttg
    44041 gattcgtata gtacgaatat gtaatatgtg tgcacaccct tataaaaatc gattaaatct
    44101 ttttcgatta tgttttttta ctaaatgtga tattcctgta ttagttgctt tttgtcaaaa
    44161 gtttttagca caggaaaagt aaaaacagaa aggaggaatg tgacaattga aagctcattt
    44221 tttcaagtat cgtggtcggc gaattcgcta tttcaatcgt tatttatggt tttgctatta
    44281 tggtttcttt ttgtttccat ttttgattct gttaggttgg tttagttgga aagtatttta
    44341 tccacgttta agtacgtttt cgaattggca aatgtatctt attcctggaa taagtattgt
    44401 gttgtatttg ttgctggtta gtgggtaatt ttagggttga tgcattggtc gcgactaaaa
    44461 gaaggatatt ttgcctctgt ttattggcgt cagttacttg ttcgaatgtt aattgacaat
    44521 cggtttgttt atacaaagaa acaagttagt gagaaacaaa caagggaaaa aatgagatta
    44581 ccaaaagttt atttttatca aaagaaacaa gaattagtgg tttcttttcc tttagatggt
    44641 gggcgttttc acgaacgatt tttaaaaatg ggtcatttgt tatctgaagt atttttgcgt
    44701 gatttaattc gtgaagaacg agaaaaagcc cgaatcaatt atgtattcct ttcagattct
    44761 ggtcggattc ctattgatca atgtatagca gaaaatggac gtattcattt aatgaaaact
    44821 ttggattggg tatatgatca atcaccaaat atgttgattg ctggtgcaat cggtggtggg
    44881 aaaacctatt tattgtattc tttaattcaa gcttgtttaa gtgttgggac agtagatatt
    44941 tgcgatggaa aagcggcaga tttagcagca ttaggtgacg tcgatgtatt caaaggacat
    45001 gtgttttaca aaataaatga agaaatgatt atttgtttac gaaatgcatt aaaagaaatg
    45061 aatcaacggt atgcctatat gaaaaccatg aaagatccta aatatgaacc agggaaaaat
    45121 tatgcttatt atgggttacc accgcatttt atcttttttg atgaatgggc agctttttat
    45181 ggttcgttgg atatggaagc gagaaataga gtcgataaat tgattcagcc attagtatta
    45241 aagggtcgtc aagcagggat gtacattatt ttagcaatgc aacgaccaga tgcagaatat
    45301 tttccaagtg gtgttcggga taatttaaca ttccgtattt ctgttggtcg attgtctccc
    45361 attggttatt taatgacttt tggagaagat tttaaaaata agccatttta caacaaagat
    45421 caacctggtc gtggatatgc ttttccagga tttgatgtac ctgtagagtt ttttgcgcca
    45481 tttgtttaca aagattatca ctttatcaaa gaatttgaga aatttactga aatgattgtc
    45541 tatgatttta cagtaaaaga ggatagtcaa gatcaaacga tgggagatga taatcagttg
    45601 ttacaagaac aactagagcg ccaaatgtat ttagcaaaag atccaaaagg aacaagtttt
    45661 tctgatgcgc atattttgta atcaactaag gaggtgaagt taaatggcag agagttgtta
    45721 ttatagagta gctacagcaa tacgaatgat taatccttct ctttcttcca gaacgtttta
    45781 tgattggttg aatcgtattg aacaagtaac tgattaccgc tttttgcgca aagaacgtgt
    45841 ttttacagga aaagttatta atcaagtatt gctaacgaaa aaagatatag aacgattgac
    45901 tcgattgtat cattatcgag tagatttaga agaggattta actttgtcaa tttatcgcgt
    45961 tttttctcct gaaaaatata gtgaaataac aaaattagat catttaattt tgtagttatt
    46021 atctatcggg agggctctag acgtagtctg gtagccgcac tctgcctggc ggtgcgcatt
    46081 aaggctgcga agcatgggcc tataatgcgc actcgctagc agcgtgtgca gctcacaaag
    46141 aggtcgtgcg cttgcgcacg gcctctttgt gtaaagcccc ccgatatata atatgggggg
    46201 cagaaataac ataacacata gaaaaaagtt ttataaaccc ttttgtatca agtgattatc
    46261 taaggtcagc agttgtacta cttttcccaa aaaacgaggg ggactagttg atatatcggt
    46321 gtttctaaag ggttaaaaaa aatcgatttt gttattttat aaaaggagcg agaaaatgac
    46381 accgattgag ctaaaaagct acagaaagaa gttaagtgca attcataata taagtggaga
    46441 taaaaagccg ctatcacaag caagatttgc gcaattgatg ggctgctcaa aagattacat
    46501 tagaaagatg gaacaaggga aaagaaaaat cccagaggat tttgaacaac gaatcaagga
    46561 acgctttcga ttgtatatga aaagcggaat agatcttcga gcaatgattg attatttgcg
    46621 tctttccttt tttgggacag actacaaaac agtgattcaa cgtgtattac gaatggatct
    46681 gacagaattt gaaacacgtc caacgggctt gtatcttttt gactgtcttt ctgttcgtgg
    46741 gaatatctgg gtgttctggc attcaaaacg aactgtaaca gaaaatgtac ttgtgcaatt
    46801 ttcaggacaa ggttgccggg aatatgagga agtcatgcgt gaacaacaaa cagattggcg
    46861 aacatggcta tattccattt ggaaaaatca aggaaagttg gaagctggtt atacacgtgt
    46921 gcaatgtagc cgattcgata ttgcgttaga tgaactttgg ggattggaaa cggaagaaca
    46981 tttcgatctc tttgaattac tagaaaaaca aaaagcaggt ttattagaaa tggactttaa
    47041 gagattccag aatattggcg gttgtttttt agatgatggc tattttcgaa gtgaaggatt
    47101 atctttgtat tttggatcgc gaaagagtcc tctctttttt aatttttatg agaaacgttt
    47161 tgaaattgcg aaccgagaaa aaatttcaga attagaagca ttaacgaaat atggtattta
    47221 taatcgatat gagttacggt tagccaatga gaaagcaaca caagcagtag aagcatttat
    47281 tttaggtgat tcaccgaaac gtttaggaga gattggcgtt ggtttaatta atgcaaaact
    47341 tattgtttgg gatagactgg aaagtgatgc acggattgtt gataaacgat gggcaaaatt
    47401 atttcgttcg atggaagaat tggattttac agtaaagcca gaaaaattct cattagatcg
    47461 ttcagaacgc tggtttttga aacaagtagc tccgacatta aagttgttac aagaagcaga
    47521 tcgtatttta ggaagagact ttgtacaaga agggattgat aatgccgaac taaatgacga
    47581 gcaacaaaac tatctagaat ggatgggaac gtcaggaggg aaaattgcat gaatgcatat
    47641 acgacaacag aatggctgcg cctgttggat ttaaaggctg caatcgaagc attaaatgaa
    47701 aagatggttg atttgtctta ttttcgtttt cgagttcctt atattgaaca agcggtaaaa
    47761 gctggacgtt atcaggaaaa agagaactgg caagaaattg cacgcttatt agaagttcgt
    47821 aagggatacg aacaagaact cgaagagttg gaatttagcc gaaggaaagg aaggctagaa
    47881 ttcattcgct tttatcgatt ttcattgcca atacctgcga ttttagcggt taaaaaaggc
    47941 tgcgataaaa tgaagatata tgaaaactgt gtggcagcac ttagcagcga aaaacctctt
    48001 atggaagaaa tttcattggt gactgtcact tgggaaatga gtcgccagcc aacagaagaa
    48061 cagacatatc tttctttaga agagattgag atagaattag aggaaattgg tcgctatgcc
    48121 acttgttcta cctattgtgg atcggtcatt agtattgctg gtgtgattgt ctaaatgaaa
    48181 aaaattatga tttatgttgg tgcgtattgg tcacgtgatc caacagtatt agaaaatcca
    48241 gaggcggtac actattgctt gcgccaatta ttctatctgt ataaagaacg attagaatcg
    48301 ttgattcgtc agttaccata tactgacaga cgtttagatg aacttcttct aaggtatcca
    48361 gcaatgtata aacgacgaaa aaatagattg ttaccagaag agtatcccat tgaaaaacat
    48421 gaacttgaag ggcgatttgt ggcttatttc tatgatgatg ttcgtatgcg cttagtagaa
    48481 caacgaatgg aaatagaaaa tgatcgctac tattttatca attattgtaa gaagcgtaag
    48541 tatcaagtaa ctgatgattt ttatgattgt attttacaag atggggaagt gattctatcc
    48601 aaaattgctc caagttttcg tgaacttgtg ccgattgatt ttttgaaaaa atgtcatttg
    48661 cggattcttc cgtaaaaaag agctataaaa ggaaagaaaa gtagaagcca aatcggtttc
    48721 tgctttttta ttttttttag gaaggagaag atggtatgtc actggaattt ttaggagagc
    48781 caattgaaaa ggaagagttc gtttcgcatt atatggattt gtttattcag gtgataaaaa
    48841 actttgtgcg attgatgaat tttatccacg cgaacaagac tatctagccg ctgaacgaat
    48901 aaaaatcagt ttattatggg aaaaattcac agatgaaaat aaccgaccgc cagattatcg
    48961 ctatttatca gattgtttaa cgaataatgt gctttctaaa gaatacctat ttcacaaacg
    49021 tgagaaaaat caaatgtcgg ttgaacattt ttgtgattgt tatattgcat tattgcgtag
    49081 tcaacagtta ttaacagtag aacgatatgg tgcagagttt ggttatgtca tgcaagctga
    49141 gcgtcataga gctacaagat gctatgaaaa agaagatcaa gtgattgatg gctatgaaga
    49201 attatgtatt cgaaacaatc gctatttgca acaacgttta tatgaacaat tgatgatgga
    49261 tttttcaaat ctctatagag ggtatgaatg ttcgatgatt caagaaaggt agaagaaaag
    49321 ggggaatttt tatgtggcga ttattatttt tggtggtatg tggtatggca ttgttctatg
    49381 tgtatcgtgt ctatctctat ccacgaatgg aacgatggac aagttggtat agagatcctt
    49441 taaaacaacg aaaaggattg aagcggtatc gttatgaatt acttgttgaa ttttcggatg
    49501 ggcgacaagc acagtattat ttttttgcaa actatccaaa atacaacacc agtgaactat
    49561 tgattcaatt attcaaaaat aatccatatg ttgggttaga agaaaacgga cgggagttct
    49621 tttatcgttc agataatatt agtgcggcgt ctatgaacaa aacacgaatt gcataaaaaa
    49681 ttggaggaat aatcatgaaa aaaacaacat tagcagtcgt aacaacacta ggaatttcat
    49741 tattttcgtt tcaattaggg agttcattgg tttatgcaga tgaaactgta gaaacaccta
    49801 agtcagaaat tgtagaaaca ttgccagggg aaggattacc agatgttgag acagagctaa
    49861 atggaggaac aacggaaaaa ccagcagaac ctgaacaatt gcagtcagag aaacctgaaa
    49921 aagagccagt gacaccagaa ccagagtttc ctgaatcaac tttaccgact gattcgatcg
    49981 aagaagaagg tgtgcatagt tcaacaacaa cggattcaag tatagtcaca actccggaac
    50041 aatcaggaac aaccagttct tctgaaaaga atactgattc gtcaagtacg gaacagccaa
    50101 cgatgtcgtc taccagtgaa gaacaaaaag aagaaccgaa acaacctgtt gaaaaatcag
    50161 aagcaattcc aaaagaaaat aaacctattg ttccagcaaa agaagtcaca gtatcggtaa
    50221 ccccaagcgg tgaaattacg acgaatactt ctcaaggaat gagtgtaccg attgtcacta
    50281 gtaatgtaga ggaattgacc catattccaa caccaaccac cccattaaaa gtagcaggtg
    50341 atcaaacaat tgttggtgta aaagatggta ttccattaat ccaagatagt gaagggaatt
    50401 tggtaaagga tttgtcgatt cctgtaaaga aacttccaag tggcaatatt gaaatcaaaa
    50461 cagcagatgg aaaaacaaaa gtcttaccaa agactggtga agaaatacat gttgcacttt
    50521 ctgttctagg aggagtgttg acaagtattg ctggatttgt tggatataaa agaaagagaa
    50581 aaggaataga aaatgagggt aattgaatgg actatttttc agaaaaacaa gcttatcgtt
    50641 gtcaatcgta cgtactagta aaaacatact agaaggagta gcttatgaaa aaacgacaac
    50701 tacggcggaa agaaaaagtt tatacagaga aagtaccgaa gcaaaagaaa atccattcaa
    50761 aaaaagcagt gaaatggatt tggtgtttgg ttttatttct ttgtttttca gggggcttgg
    50821 cattttttca atccatgaaa acgaaggttc aagtgtcgtt tttgaaagat gaaatccaac
    50881 aattacaaaa gaaagaagta caaagtcggt cagaattaac gtacacacct gaaattgaat
    50941 cctttatgaa tcgttttgtt gctttgtata tgaatacatt cgataatcat gaacaagaac
    51001 aacaacgtca agaaacatta aaaaaagagt tttatgcaca aaactttatt caagaaccag
    51061 gaagtgtttc aggtgttcga aaattaactg ctgcttcgtt tattggatta aaagaaattg
    51121 aaaccattcc aactgctagt tataaagtaa cttatgatat tcgctcgatg aatccagaga
    51181 gtaagaaaga aacaagtgtg acaatcacac aagtactcaa tcttcccttt tctatttcaa
    51241 acaagggctg tcgtattatt gcttatcctt attttacggc actaccaaag aatacagaaa
    51301 aacaacgaat gattacttat gatgacacat tatacgaaac ggttgatgcg tcggaagcga
    51361 aagatgttga aggatttgtg gaagatttct tagataagta tgcgaaaaac aatgcaaaag
    51421 atatgtcata catgatgaaa gaacctgaag gactcaatca aacagctaaa gttgatcaag
    51481 tagagatgaa agtattgcgt tataaagaaa cgaaaaaacc ttttattgta aaagcaacgg
    51541 tcgtctttcg agatggtgat acgtcacttt tacaaaaaga acaaatgacg attgtcatta
    51601 cgaaaaaaac aaatcagtat tatgt
//
LOCUS       DANJMA010000015        51343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000015 DANJMA010000000
VERSION     DANJMA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            296..1141
                     /locus_tag="PWU31_001039"
     CDS             296..1141
                     /locus_tag="PWU31_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1083598.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1422..2642
                     /locus_tag="PWU31_001040"
     CDS             1422..2642
                     /locus_tag="PWU31_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL1083599.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2639..5359
                     /locus_tag="PWU31_001041"
     CDS             2639..5359
                     /locus_tag="PWU31_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1083600.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5343..6287
                     /locus_tag="PWU31_001042"
     CDS             5343..6287
                     /locus_tag="PWU31_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1083601.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6356..7366)
                     /locus_tag="PWU31_001043"
     CDS             complement(6356..7366)
                     /locus_tag="PWU31_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1083602.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7546..7890)
                     /locus_tag="PWU31_001044"
     CDS             complement(7546..7890)
                     /locus_tag="PWU31_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL1083603.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7883..8317)
                     /locus_tag="PWU31_001045"
     CDS             complement(7883..8317)
                     /locus_tag="PWU31_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL1083604.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8455..9192
                     /locus_tag="PWU31_001046"
     CDS             8455..9192
                     /locus_tag="PWU31_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083605.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9185..10396
                     /locus_tag="PWU31_001047"
     CDS             9185..10396
                     /locus_tag="PWU31_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083606.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10600..11382
                     /locus_tag="PWU31_001048"
     CDS             10600..11382
                     /locus_tag="PWU31_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL1083607.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11452..12114
                     /gene="trmB"
                     /locus_tag="PWU31_001049"
     CDS             11452..12114
                     /gene="trmB"
                     /locus_tag="PWU31_001049"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL1083608.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12212..13966)
                     /gene="ade"
                     /locus_tag="PWU31_001050"
     CDS             complement(12212..13966)
                     /gene="ade"
                     /locus_tag="PWU31_001050"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL1083609.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14098..15027
                     /gene="tadA"
                     /locus_tag="PWU31_001051"
     CDS             14098..15027
                     /gene="tadA"
                     /locus_tag="PWU31_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL1083610.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14984..16033
                     /locus_tag="PWU31_001052"
     CDS             14984..16033
                     /locus_tag="PWU31_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL1083611.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16030..16308
                     /locus_tag="PWU31_001053"
     CDS             16030..16308
                     /locus_tag="PWU31_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL1083612.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16283..16753
                     /locus_tag="PWU31_001054"
     CDS             16283..16753
                     /locus_tag="PWU31_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL1083613.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16770..17072
                     /locus_tag="PWU31_001055"
     CDS             16770..17072
                     /locus_tag="PWU31_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083614.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17089..17460
                     /locus_tag="PWU31_001056"
     CDS             17089..17460
                     /locus_tag="PWU31_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL1083615.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17432..17791
                     /locus_tag="PWU31_001057"
     CDS             17432..17791
                     /locus_tag="PWU31_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083616.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17963..18970
                     /locus_tag="PWU31_001058"
     CDS             17963..18970
                     /locus_tag="PWU31_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1083617.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18995..20179
                     /locus_tag="PWU31_001059"
     CDS             18995..20179
                     /locus_tag="PWU31_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL1083618.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20616..22199)
                     /locus_tag="PWU31_001060"
     CDS             complement(20616..22199)
                     /locus_tag="PWU31_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1083619.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22777..22920)
                     /locus_tag="PWU31_001061"
     CDS             complement(22777..22920)
                     /locus_tag="PWU31_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL1083620.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23142..23621)
                     /locus_tag="PWU31_001062"
     CDS             complement(23142..23621)
                     /locus_tag="PWU31_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1083621.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23694..23978)
                     /locus_tag="PWU31_001063"
     CDS             complement(23694..23978)
                     /locus_tag="PWU31_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083622.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24056..24250)
                     /gene="ssrS"
                     /locus_tag="PWU31_001064"
     ncRNA           complement(24056..24250)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWU31_001064"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24322..25608)
                     /locus_tag="PWU31_001065"
     CDS             complement(24322..25608)
                     /locus_tag="PWU31_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL1083623.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25746..26417
                     /locus_tag="PWU31_001066"
     CDS             25746..26417
                     /locus_tag="PWU31_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL1083624.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26383..26871
                     /locus_tag="PWU31_001067"
     CDS             26383..26871
                     /locus_tag="PWU31_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL1083625.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26884..27831
                     /gene="prmA"
                     /locus_tag="PWU31_001068"
     CDS             26884..27831
                     /gene="prmA"
                     /locus_tag="PWU31_001068"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL1083626.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27832..28596
                     /locus_tag="PWU31_001069"
     CDS             27832..28596
                     /locus_tag="PWU31_001069"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL1083627.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28829..31042
                     /locus_tag="PWU31_001070"
     CDS             28829..31042
                     /locus_tag="PWU31_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL1083628.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31053..31499
                     /locus_tag="PWU31_001071"
     CDS             31053..31499
                     /locus_tag="PWU31_001071"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL1083629.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31670..32929
                     /locus_tag="PWU31_001072"
     CDS             31670..32929
                     /locus_tag="PWU31_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL1083630.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33018..33965)
                     /locus_tag="PWU31_001073"
     CDS             complement(33018..33965)
                     /locus_tag="PWU31_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1083631.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34359..35651
                     /locus_tag="PWU31_001074"
     CDS             34359..35651
                     /locus_tag="PWU31_001074"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL1083632.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35666..37435
                     /gene="aspS"
                     /locus_tag="PWU31_001075"
     CDS             35666..37435
                     /gene="aspS"
                     /locus_tag="PWU31_001075"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL1083633.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37544..38419
                     /locus_tag="PWU31_001076"
     CDS             37544..38419
                     /locus_tag="PWU31_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1083634.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38685..40568
                     /locus_tag="PWU31_001077"
     CDS             38685..40568
                     /locus_tag="PWU31_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1083635.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQISNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40643..40927)
                     /locus_tag="PWU31_001078"
     CDS             complement(40643..40927)
                     /locus_tag="PWU31_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1083636.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40917..41651)
                     /locus_tag="PWU31_001079"
     CDS             complement(40917..41651)
                     /locus_tag="PWU31_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL1083637.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41780..42403
                     /locus_tag="PWU31_001080"
     CDS             41780..42403
                     /locus_tag="PWU31_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL1083638.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42595..43716
                     /locus_tag="PWU31_001081"
     CDS             42595..43716
                     /locus_tag="PWU31_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL1083639.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43723..46851
                     /locus_tag="PWU31_001082"
     CDS             43723..46851
                     /locus_tag="PWU31_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL1083640.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKAEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQSNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTALVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46870..50064
                     /locus_tag="PWU31_001083"
     CDS             46870..50064
                     /locus_tag="PWU31_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL1083641.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYAEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKSKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50210..50917)
                     /locus_tag="PWU31_001084"
     CDS             complement(50210..50917)
                     /locus_tag="PWU31_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1083642.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16808 a         8956 c        10649 g        14930 t
ORIGIN      
        1 tttttgttta aatcttttgc taagttgaac taatagagtc ttttatgaat atcgaagtta
       61 gtttttcacc taacttcgtt ttttatagtt ggttattata aaatagtaat atagctgatt
      121 tggattcaat tcatatttat ttataaatca aaaatacaga gaatagtata taaatgtcct
      181 ttcctattct taaaatgatg tttgtactta cttaaattag gtataattag tatatgtttt
      241 gtgagaaatt tagacttaca ataagcagtg aatttataat caacgtatag gagacatgga
      301 tgaaatgaaa ctaattggta tagatttaga cggtactctt ttgaattctg accagagcat
      361 tagtcaaaaa aatgcaagaa caatgaaaca tttaccggaa gactgttttc cattcatttg
      421 ttctggaaga gaagtagaag acattaataa tattctcaaa aaatctggtt taaggatacc
      481 agcagtaggg ttaaatggtt ctgttggatt tgatggagaa aaaaagatat ttgagtcttc
      541 cttcgataaa aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc caaccaaaat
      601 atatacaaat ctcggaagtt atgaatctca gaattataaa aataaaatga aaaaaatttt
      661 tcacgaggta gggagtgaat tttctatcaa agagctaaac tatgagcttg agtatgaaaa
      721 atctatttgt tcaatccctt ataataatat tgaggaaatt atagaaaagg atgctatcag
      781 catttacaaa ttttttattt ttattcctaa tcgtcaattg aaagctaata tatttaacca
      841 tttgaaaaag atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt
      901 acctcaaaat gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa
      961 gaaacctttg agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc
     1021 agatattagt tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt
     1081 cactgtttca aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata
     1141 aactaattaa ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc
     1201 cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat
     1261 tgtgacaaaa atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca
     1321 tttatcgtaa tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat
     1381 tgaaaatggt atgataaaag aaaaaaagga ggaaattttt aatggtgaaa tttatccatg
     1441 cggcagattt acatatggat cggtcatttg aaggactgac aactcttgat aaaactgtac
     1501 aggaaaaact attgaagacg aatctgaccg ttttatcaaa tatcattgaa caggcaataa
     1561 taaataacgt tgattttgtt ttacttgcag gagataattt tcatcaaaat cgtccttcat
     1621 taaaaataca gaaacatttt tcagaacaga tgaaacggtt agaaaagaac catatacctg
     1681 tatttataat attcggaaat catgatttct atcaaaaaga gcggtattgg ttctcctttc
     1741 ctaaaaatgt ccatttattt acatccgaaa cggtagagac aaaaaaaata acgataaaaa
     1801 gtggagaaac tgtctctctc agtggattta gttatcgtca accgtggatt caaaaagaca
     1861 aagtgatgga atttccttct agagaactca cagattacca tattgggttg taccacgggg
     1921 aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca accctcaaag atgcaagaaa
     1981 agggatacga ttattgggct ttagggcata tccatgtgcc aacggtctta aatgaaaagc
     2041 aaacaattgt ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa gcttctacat
     2101 ctattctact agttgaatta agtaaaggaa gttgccaaat acatccgatt aaagtagcag
     2161 aggtctattg gaagacaaag gaaatatcat tgaggaaagc aagaacgaca aaagaggtca
     2221 ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg atttagttta attgagataa
     2281 aattaaaaga ttatgaccat ctcaatacag atgttttgga acggatacaa tcaggagagc
     2341 tattagacta tttgttagag gaattctcag ataggataaa tgatttcttt atttggcgta
     2401 tttctctcat agagaatact ttttatacga agaccccact tgctgcttct gctaaattga
     2461 tggaacagtt atttcaatac tatcagacac ctcaagattt ccaacaaatt ttaaatgaag
     2521 tatatagtca tcaagaagcg gctagaatat tgagcgaact gccggaatat caagaagaaa
     2581 cacttgaaaa agccaagcag ctgcttaatc aagatttttt atttgaggag gatcaggaat
     2641 gaagatttta aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt
     2701 ttattcaggc aatcagctga tctatggggg aaacgaagca ggaaaatcaa cgatttatca
     2761 atttattcaa gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac
     2821 accaaaagac ggctcagcct atggcggcaa aatatggctt aaacatcctg tatatggtga
     2881 atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga
     2941 tcaagtggga agtgatgagt tactagaaaa actgatcgca cctttgacga aagagttatt
     3001 ccagcaggta tttactttcc agcaagagca gttgatggaa ctagatcatt tgagagaaaa
     3061 agagctacat gatgcgctga tttctttagg catcacgggc agttctcaag tattccaaaa
     3121 gcggcaagaa tattatcaga aagcacaaca attattcaag aaaaaaggcc aaaaattact
     3181 aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga
     3241 acaggaagct tattttggca agttgatcca acaagaacag gaatacgagt cgcaacagca
     3301 gcagttattg gcacaattaa aagaggcgaa caaacaattt tcattagcca gacagcaaga
     3361 acttaatttt tctttatatg aagaatggca atctctcaaa aaaaacagca ctacaggtct
     3421 tcctgatgag caacttcttt tagatctgga gtctttctcc aaacggtatc agcaaacaaa
     3481 tgagcgtttg gaagaattag aagcaacact ggaaaagcat agcgggatgg atcagcagtc
     3541 agccagatat tatttttatt tagagcagga gcaggaacta aaagatctcc agcaacttca
     3601 atttacagcg gaacaattaa acgaagaatg gcaaagatta caaattgaga ctagagaaac
     3661 aaattcagaa ttgaagattt tggaacaaag gtggcattgg cagaaaaatc cgttaccgga
     3721 agaattttta tatgaagaag aatggaagca tcttctggct gaagaaagaa ggattgctga
     3781 tctagaaagc cagatttctg tgcgtctcca aatagctaaa gaacagcaag agatgattga
     3841 agcagaagtg aatgacttgg aagagaccta tccagcatta ttacaaaatc agtcagagat
     3901 agcaccagtc tcttcatcta aaccgtatat gctgatggct ggcggagcaa taatcattgc
     3961 aggtatattt gtaccaatgc ccttgaaagt gatcctttta ctaattggtg caggcatgat
     4021 tggctatttc ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt tctctgagat
     4081 aaaaggaatg tggcaagaaa aactaggaca acttgatcta gccttagctc aattagctga
     4141 agcagaagaa aaacaaaaga gaactggaga agaaaaacag attcttcagc aagaagtata
     4201 tcgttttgcg gatcatcggc acttaggaaa gatggatacg ctcaaactca tgaaagaata
     4261 tggacaggaa gtcatagatt atcagaaatt atatggcgta tttgagcgga aacataaaag
     4321 agaacaggaa atacaagaaa aactacgaca aatcgaccaa aaatttgagt ttgttaaaga
     4381 atggttaccg atacaagata agacaatttc agaaaaaatg gtattgcttt cccgttttat
     4441 acaagagatg gaacagatga aatttgccag aagctatcag caaaatactt tattgaagca
     4501 agaaatcaat caattgaaaa agaaacaaaa agtatggata caagaataca gcgaacaatt
     4561 gactcaagca ggcttatctt atccttcgga agtacctgtc tttttacaaa aagccaaaca
     4621 agccgaacag cagatgaaaa gatacaatga attatcggaa atgcttactc cactatttcc
     4681 tgaagagctg acaaaagaga aattatctgt aagactgcgc caactgaaag aaaagcaatc
     4741 ccaactccag caagaagaaa aagaagcaag tgaaaatcgg caacgactcc aacttcaagt
     4801 agaagagttg caaagagacg gcacattgga cgaactttat caacaagcta gccggcaaaa
     4861 agcaaaatta caggaattac tggaggaatg gggagcactt gaaatcgccg ggacattctt
     4921 aggtgatctt tcaacagaga tgagtgaaag acaattaccc cagttgttaa gagcagcagg
     4981 tgattattta ggtattttaa caaaaggaaa ttatcagaag atattactga aaaatgatac
     5041 actgacttta acagacggat atacagaatt tcccatctat gagttatcta caggtacgaa
     5101 agatcagctg attatggcga tgagatttgc ttacctctca ttagaagcta accgttcgat
     5161 atgcccgatc atcattgatg atggctggct tcattatgat catcagcgaa agtatcaatt
     5221 agctgaactt ttagctaaat ttggagaaaa gtatcaaata atttgttttt cttctgataa
     5281 agaaatggta agctattacc atgagatgaa ccaaatcgta aaacaattag aaggagcaaa
     5341 taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt tgaacaattt gtcctgatca
     5401 aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt actttccaag
     5461 atacttcagg tactatcgat ggtaaatact gggatgcttc ggaggaggaa atcaataaat
     5521 ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc
     5581 aagtaaagat tcttcatatg cgcttaacaa gggtagatga gccaggagag cctagtttat
     5641 acatggaaag agcccctttg aagaaagaag aaatggaaga agaaatcaat caattgttgt
     5701 ttgagatcac caatgcgcat tggaatcgta ttgttcgcta tctacttaat aaatatcaaa
     5761 aacaattttt tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc agtggattag
     5821 cttatcatac tttgacaatg cttcacttag ggaaatcaat cgccgatgaa tatcctgagc
     5881 tgaatcgagc attgttgtat gctggtatca ttttgcatga tttaggaaag gtgattgaac
     5941 tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt catctggtat
     6001 tgattgacga agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag
     6061 atgttcttgt attacgtcat atggttttag cacaccatgg cttattagaa tatggctcac
     6121 ctgtacgtcc aagaataatg gaagcagaaa tattacatca aatcgacaat atcgatgctt
     6181 cgatgcagat gatgttgact tcgattcgtc aaacagaacc aggaaaatac accgatcgga
     6241 ttttcggtat ggacaaccga agtttctatg taccaaaaga tttatagcaa aaatttattt
     6301 ttttcattca aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt ttcttttatt
     6361 ttgatgaatc agttgtttct tcagttgaag attcttttgt tgaatcagtt gattttgtgt
     6421 cagaactctt tgttgaagct gtagtttcac ttgagtcttt cgtacttgat gaagttgttg
     6481 tgaatgcact caacacattt tcaaaggcat catctttgat ttttacattt gcatctttta
     6541 attcttcacc gattacttta gttgtgaatg cattgtcact tagtttagtt tcagtagcga
     6601 tatcttttag ttgatctttg tatttatcca tgtcattgcc tttattttgg tttttcacca
     6661 ttttgacaac gtagtattct gtcgcataag aagtaggatt tgttgtagta ataacatcgg
     6721 agatctcacc gtctttcaat ttgaaagctg cttcttttac ttctgcagga atagttgttg
     6781 tcgttgagtc gaatttaact ttaccgccat cttcttttgt ctctgtatca gttgatttgt
     6841 ctttggctaa tttagagaaa tcatctccat catccgctga ttttttcaca tcttttgctt
     6901 catcttcact tgataatttg atgatttgtg cttctacttc tggatggaaa gatttccaag
     6961 cagttttcaa atcgtcatct gtaatatcta cgtgagcttt caagccagct tcaaaagcta
     7021 aattgttacg aatatattct ttataagtat ctttcgtata tccagcagct tctaattgtg
     7081 attcaaaagt atcacctaat gatttcgctt gtttgtcgta ttctgcatcc acttgcttat
     7141 cggttacttt atcgccatat ttgttattga atactttata aatgatcatc cgttgtacta
     7201 aggattggtt cgatgattca agttttgctt catcatagaa atcagataca gtgatcgttc
     7261 cgcctttcat cgttgcgata tctttattag tatcgcctga acaagctgct agagttaaaa
     7321 cagccaacgc actagtagct gccaaaataa ttgatttttt tttcattaaa atttgcactc
     7381 ctgtaattag ttattatttt tatatattaa ctataccata tcccatttcc tttaaaaacg
     7441 ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac attcctagga aatacgcaaa
     7501 cttttctcac ttatatagtt aaatctgttg agaaaataat cattattata tttgttcagg
     7561 atactctggc agatcagctt ttagtttttc tacttgttca ttgatttgtg tgatacgtgg
     7621 ttcggcttgg aatttaaaat cttcgatatc ttttttgatt gcttcttgga aagcaggaat
     7681 cacagattca gctgtttctt ttgtttcgat caatgcgctt ttaaaatgat tcaggctgtt
     7741 atttaaatca tttgtcagag ttgttgcttc ttccaattct tcgatcagtt tctttcttgt
     7801 ttcattcccg ctcctgggcg ccaataacag acctccgaca gtgccagcag cagctccaaa
     7861 taacagtcct ttgaaaaagt ttttaatcat cgcaggacac ctgctttacg attgtttctt
     7921 gaatagcttt tagttcttcc gatccataac tatcttgatg attgacaaag tgaatagaaa
     7981 aatcatcctc tttgctgtag cgagggacta aatggatatg tgaatgaaaa acagattgat
     8041 aggctagttc acggttgtta ttcaagatat taagaccttt catttctgga aaagcttttt
     8101 ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc tcctgcaagt tcgggatcat
     8161 attcaaaaat atctgctaca tgacgtttag gcacaactaa tgtgtgtcct ttcgttgctt
     8221 gagagatgtc taaaaaagca tagaccttgt cgtcttcata tattttatag cttgggattt
     8281 cttgattgat gattttgcaa aaaatacagt tttccatata aaacatcctt tctataaaga
     8341 ataaatactt cagcttaact ttaccataag ttaaagctat tgtaaggaaa aaaacaaaat
     8401 accgtcattt tcttcgtggt ataataaata aaaataaatg gcggaggaaa aagaatgagt
     8461 ttaattatcg atcatgtcac aggggggtac ggacatctcc ctgtgttaaa agatatcagt
     8521 ttcgatgtac agcctggaga gatagtaggg ttgattggac tgaacggagc agggaaaagt
     8581 acgaccatca agcacattat cggtctgctg gaagcttcaa aaggaacgat agaaatcaat
     8641 catctatcct taaagaaaaa tacggaagaa tatcgcaaac aaattgggta tattccagag
     8701 actccttctt tatatgaaga attgacattg agagaacata tcgaagtcac tgcgatggcc
     8761 tacgatatac ctatggatat agcaatgaaa agagcagaaa agttgttgga gacttttcgg
     8821 ttatctaatc gtcttgactg gttcccagct aatttttcaa agggaatgaa acagaaagtg
     8881 atgatcttat gcgccttttt gatcgagcct tctttatata tcatcgacga accattttta
     8941 ggcttggatc cactagctat caatgcattg cttgaattga tggtagaaat gaaaaaagaa
     9001 ggagccggta ttttgatgtc tacgcatatt ttagctactg cagaaaaata ttgtgatcgt
     9061 tttgtggtct tacataacgg agagatacga gcagacggtt ctcttgagat gttgagaaaa
     9121 gaatttcagc tgccggattc ttctttggac gaaatctata ttgctttaac aaaggagcag
     9181 gcacatgagt agtttttttc aaaaacgttt agcaagacat caaaagaaaa tgatgaaata
     9241 tatgcgttat gtattgaacg atcattttgt tttagtttgt cttttcttga ttggcggtgt
     9301 gggattatat tattctaatt ggttgaaaac cctgcagcca ccgtttgcaa ttggagggct
     9361 cataatctct gtattttgga tgctctgttt attcgtgggt aagatggcta cttttgcaga
     9421 ggcagcagat attgtttttt tacttcctaa agaaaaagaa atgcaaacct atttggaacg
     9481 agcttttcgc tattcttgca tttttccgtt gatctttttg atattggcat gcggattggc
     9541 catgccatta gtggtggtta gtacggagca atccttttcg atgtttttcg tctatctgat
     9601 catattatgg agtttgaaat tcagtcatct gcaaatcaag agagcagaat tgtttcgact
     9661 agatcaacaa gttattcgtt attggaaatt aggctggttt ttttcttctc tgatcttttt
     9721 gtttctatca ttttattggc taatatgggc aggaatagca ggtgcattag tacaggcaag
     9781 tctatattac tggtttttgt ggaagcaaca agaaaaagga ttggattggg agaaaatggt
     9841 gcatgtagaa gaacataggt tgcatcagat ctatcaattt atcaatttat tcacagatgt
     9901 tcctgaaatt acggcaaaga tcaaaagaag acgctatttg gatcctatat tgactcatat
     9961 taaaagagaa tcaaaaaaca cttatcttta cttgtttgca agaagattgg caagaggttc
    10021 tgattttagt ggtctttatt tccgattagt aattataggt gggttattga ttgccggagt
    10081 agtcgacttt tattttagtc tgattatcgg tttcttattt ttatatttga tcggtttcca
    10141 actgttacct ttagcgaacc agttccgcta tatggttctc gttcagcttt atccaatcca
    10201 taagaatcag aagaagcagg cgattcagta tctactcaca tgggtattaa ttatcgcgtc
    10261 ggttattttt ggtttgattg ctgcagtcgt attagatggg cagcagcgat gggttccgct
    10321 agcggcttat ttgattgtta ctatgctctt tgttaaacta tatgtaccaa ctcgtttgaa
    10381 gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa tgagaatgaa aaatagagcg
    10441 ttgacagtaa gaatgctgtt cgttctattt tttttgagaa aagctggttt tttgaatggc
    10501 aattaaatga aaaagaacgt atatgtgctt gacgtgtagt gttcaggcga ctagaataat
    10561 aagtactgca aaacaaagag taagaggacg ggagaatgca tgatggagtt tcagttagac
    10621 caagagtggc ggttacgtcc catcaaaggc gacacaggaa aaacatacat aggcatgcgt
    10681 gatgaggaca aagtctttat taaacgaaac acgactccga tgttggctgc tctttcaagg
    10741 gaaggaatta caccaaaact tgtttggaca aaacgaacgg ggaatggcga tactttgaca
    10801 gcacaagagt ggctggaagg tcgcttgctg actcctaatg agattggtca aagaaacgac
    10861 gtgattgatg ttttgtacca tttacatcat tcaaattctc ttaaaaccat gctgacccgt
    10921 atcggtggtc gtgtcatgac gccggagaag atgctgcaaa aatatgaaaa agaattaccg
    10981 gaagaactcc agaaaaatca atttctgcaa attgtccatc gttttcttcg tgagcagatc
    11041 ccgtcaatag ataaggaaaa cttatcagta gtacacggag atgtaaacca tcgtaattgg
    11101 ttagtatgta aaaattattt atatctagtc gattgggatt caatcatgtt tgcagaccct
    11161 gcggtggata ttgcaacgat tttaggaaat tatgtgccac tgtctagctg gaatcagtgg
    11221 ctgatcagtt atggcatacg cccgactgac gaaatgcttg aaaaatttca ttggtatgcg
    11281 gccatgaatt tgctccaaga aatcaccaga tattgcaaac gtgaagatca tcgacggatg
    11341 aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct gaggtttggc ccaaactctg
    11401 cggagatttg gccagacctt tttaattaga ggaaaggaat ggagataaat aatgcgtgtc
    11461 cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc cgaattttgt aattagtgat
    11521 ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa acgatcatcc aatccacatt
    11581 gagattggta tggggaaagg ccagttcatc actggaatgg caaaagccca tcctgaaatc
    11641 aattatattg gagtagaaat gcaggtcagt gttgtttcga ttgcactaga caagttgatc
    11701 gaacaaccgc ttcctaacct gaaacttcta catgtggatg gatcagcgtt gacagagtat
    11761 ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt ccgatccatg gccaaaaaaa
    11821 cgtcatgaaa aacggcgatt gacatataag acttttcttg ctgtagatga acagattctt
    11881 cgtccaaatg gagaaatcca tttcaaaaca gataatcaag gattgtttga gtattctctg
    11941 gcaagttttt ctcaatatgg catgatcctc aaacaggtat ggttagattt acatcaaagt
    12001 caatttgaag gcaatattat gacggagtat gaagaaaaat tttcttcaaa aggtcaacgt
    12061 atttatcgag tagaagctca atttcaagat aagaccaaaa agaaaagtct gtgaaactaa
    12121 ctttctgctt tcataaaaac attaatatcc ataaataaat ctcagaaaat gaacattaca
    12181 gatgtaatgt attttctggg atttatttga tttaaaatgt atttatttca tcggtttttc
    12241 cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa tcctttgtcg gtgatcttca
    12301 attcgggtat gacagataat gttaagaaag aaagtgtcaa aaaaggatca aaggttttag
    12361 ctgctcctag ttgatgtgct ttagttatta attcattcaa ctgttcatag acgatcgaaa
    12421 aaggttcctg tgtcatgatt ccgcctatag gaagagaaac acaagctatt tcctgctgtt
    12481 tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag attagcagca tacagcatat
    12541 cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc atgggcaatc gttgtcgcta
    12601 tcgctccttg tttcagctga aatcctttca ctatgcctaa tccaatgttt cccgtttgat
    12661 ggtgacgctc gatcaccgca atctttaatt ggtctttttc gattgaagga atgaatcgtt
    12721 cgccttgtcg gtccactttt tctaccaaat ctttagtcac tagactattc gggataattt
    12781 cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa cgaatgtgga tttaatggtt
    12841 gagcgtttag ttttggcaga caacacgtat atgaatttga atgatcagca aataaatggt
    12901 cttggagttg cccagattga acaactaatg ttcccttata gaaaacttca tgaattggca
    12961 gcttggtaag atcgtctgtt aagaagaaat cagcttgata cccaggggca atcgcaccta
    13021 gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc catctgaatg gcttgtaatg
    13081 gatcgattcc aagtgcaata gcttgacgga cgatatgatc gattgatcct tcttttacta
    13141 aatcattgat cagtttatca tcggtgacaa agaagcatct tcttgaattt tcaggtgtga
    13201 tcgccggtag tagcgatgcc agatcttttg caacggtccc ttctcgaacc attaaataca
    13261 taccaagatc caaacgttct ttagcttctt ttgcagttgt agcttcatga tccgtccgaa
    13321 tacctgctgc aggataaaca tttaactctt ccatgccgat accagctgca tgcccgtcaa
    13381 tttttttctt atgctggtgc atcaattgga tcttttctag gatgtctgtt tcattatttg
    13441 ctactgcttg atagttcatg acttcagcta agcctaaaac ttcgggtttt tgtaaaaaag
    13501 gggctaattt ttttgcattt aaatttgcac cattggtctc aaaaggggtt acaggtacac
    13561 aagaaggtag cataaaaaag atattcatga gtgtttgtct tgcatcttct atcataaact
    13621 caagacctgt actcccagca atgtttccta tttcatgggg atctgtcact acagtagtaa
    13681 ctccatgaag caaggatact tttgaaaatt cgcttggtga taaaagtgag ctttcgatat
    13741 gcacatggcc atcgatgaat cctggcacta gatattgccc attagcatta tgtacgcttt
    13801 ttccttcgta ttcaccgatt cctacgatca ttccttcgca gattgctaca tctttttcca
    13861 tgatttcttt cgtaaagacg ttgacgatct tagcatgctt gatcactagg tcagcaggct
    13921 tttttttact tgctacttcg atatatttcc ttaatgttgt ttccaatgga gtgcctccca
    13981 agataaattt ttataagtat aacataaatt cggcttttgc caatattttt ttgtaaattc
    14041 attttatttg aaacaaaaag atcaaaaaga cgtatgtttt ttgtggaggg gagaataatg
    14101 gaaataaaac gattatcgga tcaaatgata agtgaaagtt ttgccaaaca tgcccaagac
    14161 gtatatatat tgccagcttc agaagtattt tacatctatt ttcgctatgg aaaaaaacga
    14221 acattctgga agaaagtctc ttcggcagat ggacagcaac tgatctcaag atttaagtat
    14281 ttgggacaga tggatatcgg agaaaaaaga aaagctcagt taggtgcatt tacttattct
    14341 ttagaaaaac aggaagttcg tctgcggttg tcttctgtag gagattacct tcaacgagag
    14401 agtttagtga ttcggctatt atacggtact tttgaaacac cccatcgttg ctttagttct
    14461 cgagattttg caactatttt acagaaaacc gtcagacgtg gattatacct tttcagcgga
    14521 ccagtcggat caggcaagac ttctctaatg tatcggttgg ctttggaaga aaaattgcaa
    14581 gtgattacta tcgaagatcc tgtagagata gaagaaccgt tttttctgca gctacagatc
    14641 aatccgaaaa tagcacagac ttatgataca cttttgaaac tcgcattacg gcatcgtcca
    14701 gacttgctaa tcattggcga gataagagat gaatcaactg cacatgctgc ggtaagagca
    14761 gcactaacag ggcaccgggt ttttgcaacc gtacatgcac gcaatcttga aggtacattg
    14821 gtcagaatcc atgaactgac aaagaaaaaa gaagagctat ctgaatgttt agctggtgtc
    14881 atttatcaag aacttttgga agatgccaat caccatcctt ctgttttatg gagttatcaa
    14941 tttatggagg aagaatcagt aaaaaaatca tgggaggaaa gttatgaaga agctcaggaa
    15001 aatatttggc agtcagaact attctaaagg attaacaaga agccagcaaa accagtttgc
    15061 acaaaccatg ggagaactgc ttttatctgg ttttagtctg caagaatccc tttatttatt
    15121 actcacgatc cgttcgtttc cacctgttat tttggcaaat atccaacatc agctaaagga
    15181 agggcaagcg tttagtgagg tactttcgca aataggatat ggagcagagc aactggtaca
    15241 gatcgagtta gctgaacgtc atgggaatct gccgacaact ttgttggaaa ttgccaaaca
    15301 gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag ctgcttgcat atccgatgct
    15361 attgctcacc tttttgctag gtatcttgtt tagtatgcgg ttgttcgttt tgcctcagct
    15421 tattggttca ggaatggtca tgcaagagca ttgggggatt cagctgatac aagtctttca
    15481 ttggtatgtt ctactattca tcctagcggt tactctattg gctttactag taaggtatcg
    15541 tctgatgaag ttgtctcctg ttgatcagag tgaatggata agcagacaat tattgattgg
    15601 accgatatat tccttgtatc aatcggctta tttgtcacta gagattggaa agctttttca
    15661 tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag gaaacgaaac aagaaagtct
    15721 gattcagcat cttgcctcta agttgattca aggattggag gcaggtatgt cattggctga
    15781 gcagttccag aattatcgtt ttctatcaga agaattcagt aaaattgttt tacagggaga
    15841 ggcaaaggga agtttaggta aagaactgat tttttacagc gaatggacaa gaaaacagct
    15901 tttcaaacgt cttcatcact ggatgcattt tattcagcca atcatttttt tgggcgtagc
    15961 ggctttgatt ttattagttt atgccgctgt tttattacct gtttacggaa gcatagagga
    16021 ggttttgcca tgaaaaagag aaaaatatct tattatagcg gttttacatt gatcgaaatg
    16081 ctaatcgtct tactgatcat cagtgtatta gtgttgttgt tcgtccctaa tttgtcgcgt
    16141 tataggaatc atgtagacca agaaagtcgt gaagcgatca tccagttggt agacacacaa
    16201 aaagagctct atgcgcttca aaataatgga agagttccta ctgtggagga attgttgaat
    16261 gaaggctata tcaaacgaga acatgcagaa atctatcagc ggccttgaag gtaaggtttt
    16321 agcatcagga tatacgttgt ttgaatcact cttactgatt ttattactta caacgatcct
    16381 ttcttttcca gttctcgcat tttctgcatg gaagaatgaa ttggcagtct atcaattttt
    16441 cggccaattt gaaaaacgaa tctattctac gcaaaaaatc gctattgtga atcagatcca
    16501 aacaggcttt tattggaata gtgaagagaa tcaaatcatt tttcatgttc cgaatccaga
    16561 taaaataaat tgggaagtat tagatattcc tgatgagatt accttgaaac gccatgcttc
    16621 tattactttt gctgctagaa ccgggaatga aagttcacta aaagcttatc aattttattg
    16681 ggaagaaaaa gagcggacga ttacgtatca attccagata gggagtggtc gctatacaaa
    16741 aagaattgaa taaaacagga tttgttttgt tggagtccct tacagcgctt atgatcgctt
    16801 ctgtctgtgt ctacttagtg agctttggcc agctgttttt gcttcaggca gaaaagaaaa
    16861 gtggggagga actgctattt attcgaatga tttatgaaga tatcaagacg cagcggatgt
    16921 atcacaaaag accagagcga agggaagaaa tgatcccata cgatatccaa attgtcgata
    16981 ataatggagt aatcgaaaaa gtgtttgtca atatggagaa agaagcgatc agtattgaaa
    17041 aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa ttggactgat gatattgagc
    17101 agtctattgc tcacattcaa tcttttgatt cagcaaacca tccaagtaag tgaatttttg
    17161 cagcgtaaag atcaaaaaga gtggctaata tttttagcac aattagaaga agaactcaag
    17221 agcagtcaca atgtctctgt gaaaaatcag caactgcact acacgatcga ggataaacag
    17281 tatatcatcg aaagatatca ggcaatgata cgtaaaagaa gaacttccgg cggacatcaa
    17341 cctatgttaa caagtatcac tgagcttcaa ttattggaaa aagaacacac aatttctttt
    17401 tatgtacact ttgaaaatgg agaagatgga tatgccatat ggacaaaaaa cgatcagtag
    17461 aaaatgttat aagggctcta ttttattttc tgtcttgctt ttatttcttt tatgtacgtt
    17521 tttattgatg gcagcattag aaagctaccg tttgtcaatg gatcttaata tacgaatcaa
    17581 aaattattat atagcaaaaa taatgaagca gatggcttta tcggaacagt ctacgcttgc
    17641 cgaaagttcg gaaggagttt tttactacac aactggatct gtaacatatc agtgggtaca
    17701 acagtcccta ttcttagagg tggaagtctc accgtttatc tttcggttca cagaagaagt
    17761 aaaaaatgat acagatacta gtacagaatg atggaatgtt cgtgaaaacg ccctcttttc
    17821 tgttataaca ctgtttttaa taaaaaaatg tttgccgttt cacaaataaa aaacagtgaa
    17881 aacgattgaa attttgtcaa ttgttcgata gaattaaaca ggtgaaaaaa ttgttataat
    17941 atatctagaa atgaggtgca ccatgttacc agaaaaaatg gaacaagggt ttaatcaaaa
    18001 cctggaagct attcagcttt tacagaatgc attgggaact tcctttttag aagcctatgt
    18061 cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt gtggtggatg gagtgccgac
    18121 aaaagagaca acgcaacgga tcacagctct ttatgaagag ttgaagaaac tttctttcga
    18181 gccagaagaa tggcgcagac tgtctcaatt acttttattg aagggcagcc agacagaaca
    18241 tttgcaggcg aaccatcaac tcactcctga tagtatcggt tttttatttg tttttcttat
    18301 tgaacaacta tacacaaata aaaaagcacc ggttaagatt ctggatattg cggcaggaat
    18361 gggcaacttg ttattaacag ttcttttaaa cctatcgaat gcggggtacc aaacagaagg
    18421 aattggtgtg gatattgatg atacattgtt ggctgtcgca gcttctacaa gtgatttgac
    18481 acaagcaaat gttcaatact ttcatcaaga cgggttgcag gaattattaa tagatcctgt
    18541 cgattttgca atcagcgatt tgccaatcgg ttattatccg aatgacgaaa aagcaaaaga
    18601 gtttttaaca agtaccgaag aaggccatag ttatgcacat catttattgc tagagcaatc
    18661 aatgaaatat gtgaagccgg atggttttgg tttgtttttg atgccgagtg gatttttaga
    18721 aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa gaaggttatc ttcaaggaat
    18781 gattcaattg ccagatgaat tgttccgtaa taaacaatcg caaaaaagta ttttgatctt
    18841 acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc ttgcttgtga aacttgcttc
    18901 attaaaagaa ccagagaagg tgacggaatt tttcaacgag tttaaaaact ggaaatcatc
    18961 tgctctgtaa tcattcaacg agaaggagag tcatatgtct aaaacaattg caatcaatgc
    19021 tgggagttca agtttgaaat ggcaattata tcaaatgcca gaagaaaccg tagttgcgaa
    19081 agggatcgtc gaacgtatcg gtttacaaga ttctattttt accatcaagt acggcgataa
    19141 tcaaaaattc gaacaaatca tggatatcga aaaccacgaa gtagcagtaa aaatgctgtt
    19201 agatcagttg attgaactgc aaattttaag ctcttatgat gaaatcaccg gagtagggca
    19261 ccgtgttgtt cacggaggag aaacttatgg ggattcagta gtgatcgatg agaaagtaat
    19321 ggaccgcatt gctgaattag cagaatttgc tcctttgcat aatcctgcaa acttaatggg
    19381 gatcaaagcg tttaaaaaga ttttacctga cattttgagt gtggctgtat tcgatacatc
    19441 tttccactca acaatgccaa agcaaaatta tttatatagc ttaccggtgt cttattatga
    19501 agatttcaaa gtaagaaaat atggcttcca tggcacaagt catcgttatg tgtcggaacg
    19561 tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa atcatcactt gccacttagg
    19621 aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc atggatactt ctatgggatt
    19681 cacaccatta gctggggtga caatgggtac acgttcaggt gatatcgatc cagctgttct
    19741 cccatattta atggaaaaat tagagattga tattgatgaa atgatcaatg ttttgaacaa
    19801 gaaatctggt ctgctaggcc taacaggatt gtctagtgat atgcgtgatc ttgaaaagaa
    19861 ctatgaaaaa gaacatattc gtttagctta tgacattttc gtggaccgca tccgaaaata
    19921 catcggcggt tatgtgacaa cgatgaatgg tgttgatgca attgtcttta ctgctggtat
    19981 cggcgagaat gacggacatg tacgtagtga aatcatcaaa ggtatgtctt ggtttggttg
    20041 tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa gtagatatct ctacaaaaga
    20101 atcaaaagta agagtattag tgatcccaac agatgaggaa ctaatgattg cccgtgatgt
    20161 ggagcgtctg cgcggctaag ccaaagcatt cgttaggata aaagatgaaa cgcatgcaag
    20221 agggaattct tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa tttaacttca
    20281 atttcccttc ttttttacag gatcttctta gggaattttg cttggaaaag tggtcaaaag
    20341 ggaaaagact ggcattttat ttattgattt tcaggaaaat cccaatattt tattttcatt
    20401 catctttata agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg
    20461 ggctgtcgac tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt
    20521 gatcgaataa taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg
    20581 tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc
    20641 tggcggctga tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag
    20701 tagcggtcat ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt
    20761 tcttctcgag catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt
    20821 ttttctaaaa agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg
    20881 atccgtttac ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact
    20941 tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac
    21001 tgcagctgct gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg
    21061 taaggctttt cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg
    21121 ccgatgatgg ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact
    21181 tgatcagcgt actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca
    21241 ccattagaca agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt
    21301 ttcgccaggc ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca
    21361 tgtgcccact gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc
    21421 agttcgtaat tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg
    21481 tagtttccgg gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact
    21541 tgatcgagaa attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa
    21601 tagttttcca accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa
    21661 agtaaatcgg catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg
    21721 ctaagttctg ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg caagatcgct
    21781 gcaatccggt cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg
    21841 taacgggcca gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga
    21901 ctcatttgct gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg
    21961 gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga
    22021 ctgataacac cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg
    22081 ccagttccgt tgataccgac taaggcaatt ttttcaccag cttcaatttg acaggataaa
    22141 tcctcaaata atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg
    22201 atctctctcc ttgagaccac tcaaaaaggc catggttatc cctggcctgt gttatttaaa
    22261 ctcagattat catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg
    22321 ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga
    22381 tatggtatcg ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt
    22441 ccctccttcg tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc
    22501 gctgacattt acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga
    22561 ctgagggtaa tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg
    22621 aaatcatatg gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta
    22681 ataccgttgc attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt
    22741 atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa
    22801 tgtagcactt gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa
    22861 atcggtatcc gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac
    22921 attgacgaac caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta
    22981 atcgccctgg tgttcacttt ttttatttta cttgaactca ttgactgttt tccctttatt
    23041 ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt
    23101 gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt ggtgtggtct
    23161 ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca agctgcgttt
    23221 ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt caatcctgtg
    23281 gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc aataatttgt
    23341 tttggtgagc catattctac gacagaggtt acattattca agccagcttt cttcgcattg
    23401 ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc aagcattcca
    23461 tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa tagaagttct
    23521 ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc ttcatcagaa
    23581 ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca ctcctcaagt
    23641 caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat tatttactgc
    23701 tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac aataattacg
    23761 atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa aatacccatt
    23821 acgcctaaaa cagcatatgc agcaccgtaa aattgtagga atccttgatt tttatcattt
    23881 ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct aacgaataat
    23941 aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc caaaataagc
    24001 aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat
    24061 ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag caggtgggtc
    24121 ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac
    24181 gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc
    24241 acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca
    24301 gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact cttttatacg
    24361 gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct gataatactg
    24421 agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat caaaatgatg
    24481 aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat ggctatctct
    24541 caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa
    24601 tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga cgtctgctag
    24661 tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt tcactgtacg
    24721 agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca tcagacgccg
    24781 gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa gtgcagcatc
    24841 cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat gagcatctcc
    24901 atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg acagtgtaag
    24961 atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca gcccattcaa
    25021 tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa
    25081 ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt cgactgccaa
    25141 ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc
    25201 tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga ttcggccact
    25261 ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa
    25321 taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta gatccttttt
    25381 tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg cgatcgcact
    25441 tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag agagcatgcg
    25501 agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct gaccaacgac
    25561 ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag gattttcctc
    25621 cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag aaagaacagc
    25681 cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga
    25741 gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg acgccagaag
    25801 tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga agattagctg
    25861 gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc catagctatg
    25921 gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt
    25981 acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg attcctcaag
    26041 gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt aaaaatcgtg
    26101 gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa gcattagcaa
    26161 ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta gtttttgaga
    26221 aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca aacaaaggtg
    26281 tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc tctttacaaa
    26341 gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta
    26401 ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt agctttagat
    26461 tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga aaataaagca
    26521 caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa
    26581 gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa ttatctagca
    26641 gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc tttagtggaa
    26701 tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt ggatgaagac
    26761 gatgacgaag ctgtattcga gcttcactgg gataatcaac aactagcaga attagtagaa
    26821 gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta aggagtgaca
    26881 gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga agcagttgct
    26941 aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt agatgttgaa
    27001 cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac atcgctgaaa
    27061 gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga aatcttgcct
    27121 tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg aaaaaataaa
    27181 gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa atactatcat
    27241 ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta ccagacagaa
    27301 gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac aggcactcat
    27361 cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg tgaaacattg
    27421 ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct tggtgcaaaa
    27481 gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga aaatatggat
    27541 atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac aggaatcgag
    27601 attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatccca
    27661 gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat cattgaagag
    27721 aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga tcaaattttc
    27781 cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcgg
    27841 tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg ggaaaactat
    27901 caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt ggcattcaat
    27961 gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt ttatctgaaa
    28021 gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc
    28081 tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt aggagcgcaa
    28141 gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg
    28201 aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc ccatcggcag
    28261 atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg gctagatgat
    28321 tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca
    28381 gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt tggaccagaa
    28441 ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat actttgtgga
    28501 ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag tgctgctagc
    28561 tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat aaaagagtat
    28621 cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat tatttataca
    28681 atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg
    28741 tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat tcccgtttta
    28801 ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa cgggacccgg
    28861 tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg ttgagaaagc
    28921 atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg aaccctacat
    28981 tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc cccatacagt
    29041 ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag aagatctgag
    29101 agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat tagggaaaat
    29161 aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc tgctagcaat
    29221 ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata acatgcgtac
    29281 gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt tagaaatcta
    29341 tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag aagatacagc
    29401 acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc agacgaagag
    29461 agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag cgacagaaga
    29521 gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa tctatcgtaa
    29581 aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta ttcgagtgat
    29641 cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa aatggaaacc
    29701 aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt accaatcttt
    29761 gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc gaacacagga
    29821 aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag aaggaaaaaa
    29881 tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg agatactcga
    29941 acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag gagatatctt
    30001 tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac caaaaggatc
    30061 cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa ccactggtgc
    30121 aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag atatcattga
    30181 gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat tagttgctac
    30241 tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt
    30301 gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta cgcctaaaga
    30361 tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc aaacagaaga
    30421 tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg ccaatcgtct
    30481 gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat
    30541 catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt
    30601 cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc cgattcctgg
    30661 tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc gtcgagattg
    30721 tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag tcgaatggga
    30781 agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg gctataatcg
    30841 ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag
    30901 cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg ttggtatcca
    30961 gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg atgtctacag
    31021 tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa cgggtaagta
    31081 aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc ctacttgttt
    31141 tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta aaaaaaattg
    31201 cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg
    31261 gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa aaaggaaatc
    31321 gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac gaatcattca
    31381 atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt
    31441 cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa aacaaataaa
    31501 gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag actgataaac
    31561 tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa
    31621 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
    31681 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
    31741 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
    31801 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
    31861 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
    31921 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
    31981 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
    32041 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
    32101 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
    32161 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
    32221 tgtcgagaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
    32281 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
    32341 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
    32401 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
    32461 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
    32521 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
    32581 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
    32641 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
    32701 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
    32761 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
    32821 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
    32881 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
    32941 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
    33001 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
    33061 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
    33121 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
    33181 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
    33241 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
    33301 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
    33361 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
    33421 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
    33481 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
    33541 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
    33601 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
    33661 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
    33721 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
    33781 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
    33841 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
    33901 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
    33961 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
    34021 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
    34081 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
    34141 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
    34201 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
    34261 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
    34321 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
    34381 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
    34441 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
    34501 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
    34561 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
    34621 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
    34681 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
    34741 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
    34801 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
    34861 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
    34921 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
    34981 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
    35041 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
    35101 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
    35161 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
    35221 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
    35281 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
    35341 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
    35401 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
    35461 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
    35521 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
    35581 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
    35641 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
    35701 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
    35761 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
    35821 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
    35881 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
    35941 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
    36001 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
    36061 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
    36121 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
    36181 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
    36241 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
    36301 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
    36361 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
    36421 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
    36481 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
    36541 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
    36601 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
    36661 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
    36721 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
    36781 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
    36841 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
    36901 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
    36961 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
    37021 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
    37081 agaagcttat gatatcgtct tgaacggtta tgaattaggc ggaggttcta tccgtattca
    37141 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
    37201 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
    37261 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
    37321 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
    37381 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
    37441 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
    37501 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
    37561 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
    37621 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
    37681 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
    37741 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
    37801 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
    37861 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
    37921 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
    37981 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
    38041 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
    38101 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
    38161 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
    38221 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
    38281 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
    38341 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
    38401 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
    38461 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
    38521 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
    38581 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
    38641 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
    38701 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
    38761 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
    38821 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
    38881 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
    38941 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
    39001 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
    39061 gtaacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
    39121 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
    39181 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
    39241 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
    39301 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
    39361 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
    39421 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
    39481 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
    39541 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
    39601 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
    39661 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
    39721 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
    39781 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
    39841 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
    39901 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
    39961 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
    40021 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
    40081 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
    40141 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
    40201 aggaacaaga tttgttgaaa gacactgttc tgattggact aggaacgaat ggctcattct
    40261 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
    40321 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
    40381 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
    40441 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
    40501 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
    40561 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
    40621 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
    40681 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
    40741 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
    40801 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
    40861 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
    40921 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
    40981 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
    41041 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
    41101 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
    41161 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
    41221 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
    41281 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
    41341 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
    41401 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
    41461 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
    41521 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
    41581 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    41641 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    41701 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    41761 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    41821 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    41881 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    41941 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    42001 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    42061 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    42121 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    42181 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    42241 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    42301 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    42361 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    42421 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    42481 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    42541 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    42601 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    42661 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    42721 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    42781 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    42841 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    42901 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    42961 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    43021 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    43081 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    43141 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    43201 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    43261 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    43321 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    43381 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    43441 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    43501 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    43561 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    43621 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    43681 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    43741 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    43801 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    43861 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    43921 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    43981 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    44041 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    44101 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    44161 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    44221 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    44281 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    44341 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    44401 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    44461 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    44521 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    44581 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    44641 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagct
    44701 gaaaaaatag aactgtcgca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    44761 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    44821 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    44881 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    44941 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    45001 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    45061 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    45121 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    45181 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    45241 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    45301 cccatacaac aattaaaaca cgaacagtca aataaggaag atatcttaaa agtagaacaa
    45361 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    45421 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    45481 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    45541 gagataacag aacaaaaaac aaaaacagct cttgtagtaa tcgaacaatc tttagataga
    45601 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    45661 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    45721 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    45781 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    45841 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    45901 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    45961 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    46021 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    46081 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    46141 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    46201 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    46261 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    46321 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    46381 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    46441 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    46501 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccttgtct
    46561 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    46621 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    46681 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    46741 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    46801 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    46861 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    46921 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    46981 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    47041 tgccaatgtc cttattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    47101 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    47161 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    47221 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    47281 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    47341 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacgcagaa
    47401 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    47461 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    47521 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    47581 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    47641 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    47701 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    47761 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    47821 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    47881 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctttc agagatcggc
    47941 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    48001 ttctttttcc gaaaatcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggtgaa
    48061 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    48121 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    48181 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    48241 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    48301 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    48361 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    48421 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    48481 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    48541 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    48601 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    48661 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    48721 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    48781 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    48841 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    48901 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    48961 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    49021 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    49081 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    49141 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    49201 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    49261 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    49321 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    49381 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    49441 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    49501 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    49561 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    49621 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    49681 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    49741 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    49801 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    49861 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    49921 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    49981 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    50041 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    50101 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    50161 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    50221 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    50281 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    50341 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    50401 gaaattcttt ctcagccagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    50461 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    50521 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    50581 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    50641 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    50701 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    50761 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    50821 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    50881 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    50941 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    51001 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    51061 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    51121 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    51181 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    51241 tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacaga
    51301 gaaaataagc gttataataa agaacagtat tatttaaaat gac
//
LOCUS       DANJMA010000016        50128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000016 DANJMA010000000
VERSION     DANJMA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(8..319)
                     /locus_tag="PWU31_001085"
     CDS             complement(8..319)
                     /locus_tag="PWU31_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1083643.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(321..518)
                     /locus_tag="PWU31_001086"
     CDS             complement(321..518)
                     /locus_tag="PWU31_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL1083644.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(912..2639)
                     /locus_tag="PWU31_001087"
     CDS             complement(912..2639)
                     /locus_tag="PWU31_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL1083645.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2632..3825)
                     /locus_tag="PWU31_001088"
     CDS             complement(2632..3825)
                     /locus_tag="PWU31_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1083646.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4012..4656)
                     /locus_tag="PWU31_001089"
     CDS             complement(4012..4656)
                     /locus_tag="PWU31_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1083647.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4750..4884)
                     /locus_tag="PWU31_001090"
     CDS             complement(4750..4884)
                     /locus_tag="PWU31_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL1083648.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4886..7018)
                     /gene="feoB"
                     /locus_tag="PWU31_001091"
     CDS             complement(4886..7018)
                     /gene="feoB"
                     /locus_tag="PWU31_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL1083649.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7015..7488)
                     /locus_tag="PWU31_001092"
     CDS             complement(7015..7488)
                     /locus_tag="PWU31_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL1083650.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7889..8113
                     /gene="nrdH"
                     /locus_tag="PWU31_001093"
     CDS             7889..8113
                     /gene="nrdH"
                     /locus_tag="PWU31_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL1083651.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8272..10431
                     /gene="nrdE"
                     /locus_tag="PWU31_001094"
     CDS             8272..10431
                     /gene="nrdE"
                     /locus_tag="PWU31_001094"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL1083652.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10481..11446
                     /gene="nrdF"
                     /locus_tag="PWU31_001095"
     CDS             10481..11446
                     /gene="nrdF"
                     /locus_tag="PWU31_001095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL1083653.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11535..12674)
                     /locus_tag="PWU31_001096"
     CDS             complement(11535..12674)
                     /locus_tag="PWU31_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1083654.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12983..13696)
                     /locus_tag="PWU31_001097"
     CDS             complement(12983..13696)
                     /locus_tag="PWU31_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL1083655.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13950..14651)
                     /gene="nagB"
                     /locus_tag="PWU31_001098"
     CDS             complement(13950..14651)
                     /gene="nagB"
                     /locus_tag="PWU31_001098"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL1083656.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14885..16417
                     /locus_tag="PWU31_001099"
     CDS             14885..16417
                     /locus_tag="PWU31_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1083657.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16608..17810)
                     /locus_tag="PWU31_001100"
     CDS             complement(16608..17810)
                     /locus_tag="PWU31_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1083658.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17892..18581)
                     /locus_tag="PWU31_001101"
     CDS             complement(17892..18581)
                     /locus_tag="PWU31_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1083659.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18687..19055)
                     /gene="rplL"
                     /locus_tag="PWU31_001102"
     CDS             complement(18687..19055)
                     /gene="rplL"
                     /locus_tag="PWU31_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL1083660.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19108..19611)
                     /locus_tag="PWU31_001103"
     CDS             complement(19108..19611)
                     /locus_tag="PWU31_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL1083661.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19815..19942)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20021..20710)
                     /gene="rplA"
                     /locus_tag="PWU31_001104"
     CDS             complement(20021..20710)
                     /gene="rplA"
                     /locus_tag="PWU31_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL1083662.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20814..21236)
                     /gene="rplK"
                     /locus_tag="PWU31_001105"
     CDS             complement(20814..21236)
                     /gene="rplK"
                     /locus_tag="PWU31_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL1083663.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21579..22247
                     /gene="sdaAB"
                     /locus_tag="PWU31_001106"
     CDS             21579..22247
                     /gene="sdaAB"
                     /locus_tag="PWU31_001106"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL1083664.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22260..23132
                     /gene="sdaAA"
                     /locus_tag="PWU31_001107"
     CDS             22260..23132
                     /gene="sdaAA"
                     /locus_tag="PWU31_001107"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL1083665.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23223..24242)
                     /locus_tag="PWU31_001108"
     CDS             complement(23223..24242)
                     /locus_tag="PWU31_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL1083666.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24415..25320)
                     /locus_tag="PWU31_001109"
     CDS             complement(24415..25320)
                     /locus_tag="PWU31_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1083667.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25379..25924)
                     /gene="nusG"
                     /locus_tag="PWU31_001110"
     CDS             complement(25379..25924)
                     /gene="nusG"
                     /locus_tag="PWU31_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL1083668.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26025..26195)
                     /gene="secE"
                     /locus_tag="PWU31_001111"
     CDS             complement(26025..26195)
                     /gene="secE"
                     /locus_tag="PWU31_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL1083669.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26219..26371)
                     /gene="rpmG"
                     /locus_tag="PWU31_001112"
     CDS             complement(26219..26371)
                     /gene="rpmG"
                     /locus_tag="PWU31_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1083670.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26533..27177
                     /locus_tag="PWU31_001113"
     CDS             26533..27177
                     /locus_tag="PWU31_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1083671.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27233..27736)
                     /locus_tag="PWU31_001114"
     CDS             complement(27233..27736)
                     /locus_tag="PWU31_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL1083672.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27749..27795)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28333..29298
                     /locus_tag="PWU31_001115"
     CDS             28333..29298
                     /locus_tag="PWU31_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL1083673.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29397..30569)
                     /locus_tag="PWU31_001116"
     CDS             complement(29397..30569)
                     /locus_tag="PWU31_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083674.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30556..31701)
                     /locus_tag="PWU31_001117"
     CDS             complement(30556..31701)
                     /locus_tag="PWU31_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083675.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31715..32639)
                     /locus_tag="PWU31_001118"
                     /pseudo
     CDS             complement(31715..32639)
                     /locus_tag="PWU31_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32756..33835
                     /locus_tag="PWU31_001119"
     CDS             32756..33835
                     /locus_tag="PWU31_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1083676.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33867..34460
                     /locus_tag="PWU31_001120"
     CDS             33867..34460
                     /locus_tag="PWU31_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1083677.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34570..34797)
                     /locus_tag="PWU31_001121"
     CDS             complement(34570..34797)
                     /locus_tag="PWU31_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1083678.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34900..37488)
                     /locus_tag="PWU31_001122"
     CDS             complement(34900..37488)
                     /locus_tag="PWU31_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL1083679.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37508..38167)
                     /locus_tag="PWU31_001123"
     CDS             complement(37508..38167)
                     /locus_tag="PWU31_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1083680.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38216..38719)
                     /gene="trmL"
                     /locus_tag="PWU31_001124"
     CDS             complement(38216..38719)
                     /gene="trmL"
                     /locus_tag="PWU31_001124"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL1083681.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38940..39806)
                     /locus_tag="PWU31_001125"
     CDS             complement(38940..39806)
                     /locus_tag="PWU31_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1083682.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39915..40556
                     /locus_tag="PWU31_001126"
     CDS             39915..40556
                     /locus_tag="PWU31_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL1083683.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40618..41979)
                     /gene="mgtE"
                     /locus_tag="PWU31_001127"
     CDS             complement(40618..41979)
                     /gene="mgtE"
                     /locus_tag="PWU31_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL1083684.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41997..42896)
                     /locus_tag="PWU31_001128"
     CDS             complement(41997..42896)
                     /locus_tag="PWU31_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1083685.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42898..43695)
                     /locus_tag="PWU31_001129"
     CDS             complement(42898..43695)
                     /locus_tag="PWU31_001129"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL1083686.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43673..44377)
                     /locus_tag="PWU31_001130"
     CDS             complement(43673..44377)
                     /locus_tag="PWU31_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL1083687.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44508..45083
                     /locus_tag="PWU31_001131"
     CDS             44508..45083
                     /locus_tag="PWU31_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL1083688.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            45201..45878
                     /locus_tag="PWU31_001132"
     CDS             45201..45878
                     /locus_tag="PWU31_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1083689.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45957..47765)
                     /gene="pepF"
                     /locus_tag="PWU31_001133"
     CDS             complement(45957..47765)
                     /gene="pepF"
                     /locus_tag="PWU31_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL1083690.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47941..48939)
                     /locus_tag="PWU31_001134"
     CDS             complement(47941..48939)
                     /locus_tag="PWU31_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL1083691.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49165..49818)
                     /locus_tag="PWU31_001135"
     CDS             complement(49165..49818)
                     /locus_tag="PWU31_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL1083692.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15010 a        10511 c         8784 g        15823 t
ORIGIN      
        1 agtatcatta aaattgagat ttatctccat aatctgctcg atataaacca tttattccag
       61 cagttcttcc aatttcactc aaatttctta ttaattcctc ttgatattca gtgaatctta
      121 ttttattagt ataagcagta aaatatagat aattcaccaa tctatttatt aaaggaccgg
      181 gatctaattc atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa
      241 tatcagaata tttaggatct ttacttatag atatatcatt atataattca tgaacaattt
      301 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
      361 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
      421 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
      481 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
      541 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
      601 gtagcaaaat aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat
      661 tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt
      721 catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc
      781 tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga
      841 cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg
      901 tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct
      961 tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct
     1021 ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat
     1081 ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct
     1141 aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt
     1201 ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc
     1261 tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact
     1321 acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg
     1381 atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg
     1441 gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt
     1501 agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa
     1561 ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca
     1621 tttgttgaag caacttctgt atcccattca acataagata agttgatacg ttttgtttct
     1681 ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca
     1741 atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg
     1801 tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca
     1861 accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt
     1921 cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt
     1981 gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt
     2041 ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt
     2101 cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat
     2161 acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct
     2221 ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt
     2281 gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca
     2341 agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg
     2401 attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc
     2461 aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc
     2521 actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat
     2581 tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg
     2641 ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc
     2701 ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa
     2761 aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat
     2821 caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag
     2881 gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa
     2941 tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg
     3001 agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc
     3061 tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa
     3121 ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt
     3181 tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg
     3241 atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct
     3301 ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa
     3361 tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg
     3421 tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag
     3481 accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt
     3541 gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag
     3601 acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa
     3661 gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt
     3721 ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt
     3781 tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt
     3841 ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt
     3901 cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct
     3961 ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc
     4021 aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat
     4081 aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc
     4141 ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct
     4201 aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg
     4261 attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg
     4321 attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat
     4381 ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa
     4441 tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt
     4501 gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt
     4561 ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg
     4621 gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac
     4681 tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc
     4741 aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc
     4801 acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa
     4861 cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa
     4921 actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa
     4981 tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa
     5041 tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt
     5101 tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta
     5161 aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc
     5221 agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa
     5281 taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc
     5341 aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt
     5401 cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag
     5461 aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc
     5521 ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg
     5581 tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca
     5641 taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta
     5701 ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca
     5761 aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc
     5821 ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc
     5881 gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata
     5941 aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc
     6001 cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa
     6061 catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc
     6121 ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg
     6181 ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat
     6241 catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca
     6301 gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct
     6361 ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat
     6421 atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat
     6481 gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt
     6541 ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga
     6601 ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt
     6661 cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata
     6721 actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca
     6781 gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag
     6841 acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc
     6901 cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca
     6961 agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc
     7021 tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata
     7081 ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt
     7141 tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat
     7201 tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga
     7261 ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg
     7321 atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact
     7381 tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg
     7441 ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct
     7501 cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt
     7561 acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt
     7621 atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat
     7681 ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact
     7741 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
     7801 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
     7861 taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg
     7921 tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt
     7981 caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt
     8041 tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa
     8101 acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga
     8161 gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca
     8221 atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt
     8281 aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat
     8341 ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc
     8401 aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa
     8461 gaatatatag aagcagaatt cattcaattg tattcaccat catttattga aaagctatat
     8521 caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat
     8581 tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga
     8641 gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca
     8701 gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt
     8761 aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct
     8821 atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt
     8881 actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt
     8941 ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt
     9001 cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc
     9061 ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc
     9121 gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt
     9181 agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct
     9241 gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat
     9301 cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt
     9361 gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca
     9421 gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta
     9481 ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct
     9541 aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct
     9601 gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta
     9661 ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat
     9721 tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc
     9781 aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct
     9841 gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc
     9901 aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa
     9961 gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca
    10021 atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa
    10081 cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg
    10141 attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca
    10201 atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc
    10261 ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt
    10321 ttaagacatt acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact
    10381 gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga
    10441 tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga
    10501 agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac
    10561 agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac
    10621 attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga
    10681 tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga
    10741 agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc
    10801 tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac
    10861 gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc
    10921 gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta
    10981 tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact
    11041 aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt
    11101 caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta
    11161 tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac
    11221 agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat
    11281 ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc
    11341 aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca
    11401 tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat ttattttaat
    11461 tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga
    11521 actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc
    11581 gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat tatcgtctta
    11641 atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct
    11701 ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac
    11761 ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa
    11821 gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg
    11881 ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc
    11941 tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc
    12001 gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct
    12061 ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg
    12121 aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat
    12181 gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta
    12241 agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc
    12301 tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc
    12361 aattgaattg tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa
    12421 atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg
    12481 agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa
    12541 aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag
    12601 tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta
    12661 aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca
    12721 aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt
    12781 aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg
    12841 cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc
    12901 aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat
    12961 ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat
    13021 ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa
    13081 tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat
    13141 cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc
    13201 tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta
    13261 ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc
    13321 cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca
    13381 ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt
    13441 gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag
    13501 catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca
    13561 tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac
    13621 cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca
    13681 ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga
    13741 caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata
    13801 gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg
    13861 caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt
    13921 tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg
    13981 atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt
    14041 ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt
    14101 tctttacttt tcataattga accgattccc attgaaacag cttttgttgg tacatcttct
    14161 gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa
    14221 gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt
    14281 cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct
    14341 gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca
    14401 aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg
    14461 tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct
    14521 ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc
    14581 ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga
    14641 atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt
    14701 tttcttttta caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa
    14761 ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa
    14821 accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt
    14881 tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta
    14941 tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg
    15001 agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca
    15061 gatccattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga
    15121 aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga
    15181 aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa
    15241 agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt
    15301 tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa
    15361 tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga
    15421 tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac
    15481 gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct
    15541 tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta agtatactgg
    15601 ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata
    15661 tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg
    15721 ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac
    15781 gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac
    15841 attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat
    15901 caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa
    15961 atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt
    16021 cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga
    16081 aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg
    16141 ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag
    16201 tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact
    16261 gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg
    16321 gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga
    16381 tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa
    16441 aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt
    16501 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc
    16561 tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt
    16621 tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa
    16681 acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc
    16741 aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg
    16801 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
    16861 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
    16921 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
    16981 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
    17041 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
    17101 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
    17161 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
    17221 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
    17281 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
    17341 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
    17401 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
    17461 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
    17521 gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt
    17581 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
    17641 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
    17701 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
    17761 aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc
    17821 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
    17881 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
    17941 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
    18001 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
    18061 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
    18121 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
    18181 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
    18241 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
    18301 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
    18361 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
    18421 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
    18481 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
    18541 cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
    18601 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
    18661 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
    18721 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
    18781 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
    18841 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
    18901 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
    18961 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
    19021 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
    19081 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
    19141 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
    19201 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
    19261 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
    19321 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
    19381 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
    19441 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
    19501 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
    19561 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
    19621 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
    19681 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
    19741 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
    19801 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
    19861 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
    19921 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
    19981 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
    20041 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
    20101 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
    20161 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
    20221 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
    20281 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
    20341 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
    20401 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
    20461 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
    20521 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
    20581 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
    20641 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
    20701 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
    20761 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
    20821 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
    20881 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
    20941 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
    21001 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
    21061 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
    21121 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
    21181 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
    21241 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
    21301 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
    21361 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
    21421 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
    21481 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
    21541 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
    21601 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
    21661 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
    21721 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
    21781 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
    21841 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
    21901 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
    21961 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
    22021 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
    22081 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
    22141 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
    22201 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
    22261 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
    22321 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
    22381 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
    22441 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
    22501 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
    22561 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
    22621 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
    22681 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
    22741 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
    22801 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
    22861 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
    22921 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
    22981 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
    23041 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
    23101 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
    23161 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
    23221 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
    23281 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
    23341 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
    23401 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
    23461 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt
    23521 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
    23581 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
    23641 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
    23701 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
    23761 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
    23821 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
    23881 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
    23941 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
    24001 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
    24061 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
    24121 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
    24181 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
    24241 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
    24301 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
    24361 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
    24421 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
    24481 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
    24541 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
    24601 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
    24661 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
    24721 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
    24781 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
    24841 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
    24901 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
    24961 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
    25021 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
    25081 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
    25141 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
    25201 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
    25261 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
    25321 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
    25381 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
    25441 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    25501 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
    25561 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
    25621 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
    25681 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
    25741 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
    25801 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    25861 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    25921 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    25981 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
    26041 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
    26101 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
    26161 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
    26221 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
    26281 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
    26341 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
    26401 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
    26461 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
    26521 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
    26581 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
    26641 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    26701 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    26761 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    26821 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    26881 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    26941 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    27001 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    27061 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    27121 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    27181 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    27241 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    27301 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    27361 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    27421 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    27481 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    27541 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    27601 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    27661 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    27721 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    27781 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    27841 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    27901 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    27961 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    28021 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    28081 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
    28141 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
    28201 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
    28261 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
    28321 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
    28381 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
    28441 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
    28501 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
    28561 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    28621 gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca
    28681 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    28741 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    28801 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    28861 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    28921 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    28981 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    29041 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    29101 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    29161 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    29221 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    29281 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    29341 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    29401 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    29461 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    29521 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    29581 gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    29641 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    29701 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    29761 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    29821 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    29881 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    29941 ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    30001 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    30061 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    30121 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    30181 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc
    30241 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    30301 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    30361 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    30421 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    30481 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    30541 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    30601 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    30661 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    30721 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    30781 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    30841 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    30901 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    30961 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    31021 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    31081 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    31141 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    31201 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    31261 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    31321 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    31381 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    31441 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    31501 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    31561 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    31621 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    31681 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    31741 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    31801 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    31861 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
    31921 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    31981 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    32041 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    32101 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    32161 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    32221 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    32281 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    32341 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    32401 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    32461 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    32521 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    32581 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    32641 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    32701 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    32761 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    32821 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    32881 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    32941 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    33001 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    33061 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    33121 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    33181 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    33241 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    33301 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    33361 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    33421 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    33481 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    33541 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    33601 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    33661 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    33721 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    33781 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    33841 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
    33901 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
    33961 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
    34021 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
    34081 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
    34141 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
    34201 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
    34261 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
    34321 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
    34381 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
    34441 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
    34501 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
    34561 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
    34621 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
    34681 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
    34741 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
    34801 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
    34861 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
    34921 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
    34981 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
    35041 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
    35101 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
    35161 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
    35221 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
    35281 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
    35341 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
    35401 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
    35461 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
    35521 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
    35581 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
    35641 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
    35701 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
    35761 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
    35821 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
    35881 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
    35941 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
    36001 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
    36061 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
    36121 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
    36181 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
    36241 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
    36301 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
    36361 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
    36421 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
    36481 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
    36541 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
    36601 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
    36661 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
    36721 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
    36781 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
    36841 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
    36901 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
    36961 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
    37021 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
    37081 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
    37141 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
    37201 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
    37261 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
    37321 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
    37381 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
    37441 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
    37501 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
    37561 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
    37621 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
    37681 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
    37741 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
    37801 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
    37861 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
    37921 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
    37981 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
    38041 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
    38101 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
    38161 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
    38221 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
    38281 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
    38341 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
    38401 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
    38461 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
    38521 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
    38581 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
    38641 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
    38701 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
    38761 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
    38821 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
    38881 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
    38941 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
    39001 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
    39061 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
    39121 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
    39181 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
    39241 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
    39301 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
    39361 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
    39421 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
    39481 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
    39541 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
    39601 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
    39661 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    39721 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    39781 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    39841 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    39901 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    39961 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    40021 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    40081 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    40141 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    40201 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    40261 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    40321 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    40381 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    40441 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    40501 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    40561 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    40621 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    40681 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    40741 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    40801 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    40861 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    40921 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    40981 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    41041 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa
    41101 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    41161 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    41221 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    41281 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    41341 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    41401 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    41461 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    41521 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    41581 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    41641 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    41701 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    41761 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    41821 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    41881 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    41941 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    42001 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    42061 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    42121 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    42181 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    42241 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    42301 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    42361 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    42421 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    42481 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    42541 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    42601 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    42661 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    42721 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    42781 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    42841 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    42901 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    42961 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    43021 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    43081 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    43141 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    43201 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    43261 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    43321 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    43381 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    43441 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    43501 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    43561 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    43621 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    43681 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    43741 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    43801 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    43861 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    43921 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    43981 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    44041 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    44101 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    44161 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    44221 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    44281 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    44341 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    44401 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    44461 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    44521 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    44581 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    44641 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    44701 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    44761 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    44821 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    44881 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    44941 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    45001 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    45061 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    45121 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    45181 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    45241 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    45301 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    45361 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    45421 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    45481 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    45541 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    45601 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    45661 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    45721 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    45781 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    45841 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    45901 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    45961 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    46021 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    46081 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    46141 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    46201 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    46261 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    46321 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    46381 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    46441 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    46501 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    46561 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    46621 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    46681 tcgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    46741 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    46801 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    46861 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    46921 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    46981 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    47041 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    47101 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    47161 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    47221 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    47281 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    47341 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    47401 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    47461 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    47521 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    47581 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    47641 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    47701 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    47761 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    47821 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    47881 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    47941 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    48001 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    48061 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    48121 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    48181 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    48241 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    48301 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    48361 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    48421 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    48481 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    48541 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    48601 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    48661 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    48721 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    48781 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    48841 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    48901 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    48961 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    49021 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    49081 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    49141 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    49201 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    49261 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    49321 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    49381 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    49441 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    49501 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    49561 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    49621 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    49681 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    49741 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    49801 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    49861 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    49921 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    49981 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    50041 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    50101 tggaaaaact atctttgtaa tagtcttt
//
LOCUS       DANJMA010000017        47920 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000017 DANJMA010000000
VERSION     DANJMA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(37..1188)
                     /locus_tag="PWU31_001136"
     CDS             complement(37..1188)
                     /locus_tag="PWU31_001136"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL1083693.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="PWU31_001137"
     CDS             complement(1201..1638)
                     /locus_tag="PWU31_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1083694.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="PWU31_001138"
     CDS             complement(1653..3419)
                     /locus_tag="PWU31_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL1083695.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="PWU31_001139"
     CDS             3683..4360
                     /locus_tag="PWU31_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083696.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="PWU31_001140"
     CDS             4363..5490
                     /locus_tag="PWU31_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083697.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="PWU31_001141"
     CDS             complement(5690..6130)
                     /locus_tag="PWU31_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1083698.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="PWU31_001142"
     CDS             complement(6141..8174)
                     /locus_tag="PWU31_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1083699.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="PWU31_001143"
     CDS             complement(8176..9879)
                     /locus_tag="PWU31_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1083700.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="PWU31_001144"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="PWU31_001144"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL1083701.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="PWU31_001145"
     CDS             complement(10962..11987)
                     /locus_tag="PWU31_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL1083702.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="PWU31_001146"
     CDS             complement(12196..13065)
                     /locus_tag="PWU31_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL1083703.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="PWU31_001147"
     CDS             complement(13524..15416)
                     /locus_tag="PWU31_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083704.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="PWU31_001148"
     CDS             complement(15413..17140)
                     /locus_tag="PWU31_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083705.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="PWU31_001149"
     CDS             complement(17156..17806)
                     /locus_tag="PWU31_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1083706.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="PWU31_001150"
     CDS             18010..18306
                     /locus_tag="PWU31_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1083707.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="PWU31_001151"
     CDS             18478..19098
                     /locus_tag="PWU31_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL1083708.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="PWU31_001152"
     CDS             complement(19190..20356)
                     /locus_tag="PWU31_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1083709.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="PWU31_001153"
     CDS             complement(20368..20721)
                     /locus_tag="PWU31_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1083710.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="PWU31_001154"
     CDS             20849..21871
                     /locus_tag="PWU31_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL1083711.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="PWU31_001155"
     CDS             21890..22051
                     /locus_tag="PWU31_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083712.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="PWU31_001156"
     CDS             complement(22310..22744)
                     /locus_tag="PWU31_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083713.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="PWU31_001157"
     CDS             complement(22854..24713)
                     /locus_tag="PWU31_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1083714.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="PWU31_001158"
     CDS             complement(24831..25991)
                     /locus_tag="PWU31_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL1083715.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="PWU31_001159"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="PWU31_001159"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL1083716.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="PWU31_001160"
     CDS             complement(27738..28436)
                     /locus_tag="PWU31_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1083717.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="PWU31_001161"
     CDS             complement(28393..30054)
                     /locus_tag="PWU31_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL1083718.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="PWU31_001162"
     CDS             30272..31672
                     /locus_tag="PWU31_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL1083719.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="PWU31_001163"
     CDS             complement(31924..33003)
                     /locus_tag="PWU31_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1083720.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="PWU31_001164"
     CDS             complement(33089..34048)
                     /locus_tag="PWU31_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1083721.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="PWU31_001165"
     CDS             complement(34060..35571)
                     /locus_tag="PWU31_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL1083722.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="PWU31_001166"
     CDS             complement(35593..36156)
                     /locus_tag="PWU31_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL1083723.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="PWU31_001167"
     CDS             complement(36162..37025)
                     /locus_tag="PWU31_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1083724.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="PWU31_001168"
     CDS             complement(37039..37935)
                     /locus_tag="PWU31_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083725.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="PWU31_001169"
     CDS             complement(38110..39399)
                     /locus_tag="PWU31_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1083726.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="PWU31_001170"
     CDS             complement(39371..40888)
                     /locus_tag="PWU31_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL1083727.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="PWU31_001171"
     CDS             41086..41976
                     /locus_tag="PWU31_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL1083728.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="PWU31_001172"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="PWU31_001172"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL1083729.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="PWU31_001173"
     CDS             complement(42885..44357)
                     /locus_tag="PWU31_001173"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL1083730.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="PWU31_001174"
     CDS             complement(44994..45356)
                     /locus_tag="PWU31_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL1083731.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="PWU31_001175"
     CDS             45579..46331
                     /locus_tag="PWU31_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1083732.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="PWU31_001176"
     CDS             complement(46471..47394)
                     /locus_tag="PWU31_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1083733.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="PWU31_001177"
     CDS             complement(47409..47681)
                     /locus_tag="PWU31_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083734.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13960 a         9950 c         8261 g        15749 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca
//
LOCUS       DANJMA010000018        47471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000018 DANJMA010000000
VERSION     DANJMA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(56..790)
                     /locus_tag="PWU31_001178"
     CDS             complement(56..790)
                     /locus_tag="PWU31_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1083735.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="PWU31_001179"
     CDS             1117..1527
                     /locus_tag="PWU31_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL1083736.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="PWU31_001180"
     CDS             1520..2221
                     /locus_tag="PWU31_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL1083737.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="PWU31_001181"
     CDS             2221..3807
                     /locus_tag="PWU31_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083738.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="PWU31_001182"
     CDS             3826..4047
                     /locus_tag="PWU31_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL1083739.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="PWU31_001183"
     CDS             4044..4805
                     /locus_tag="PWU31_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL1083740.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="PWU31_001184"
     CDS             5185..5694
                     /locus_tag="PWU31_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL1083741.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="PWU31_001185"
     CDS             complement(5769..6308)
                     /locus_tag="PWU31_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL1083742.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="PWU31_001186"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="PWU31_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL1083743.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="PWU31_001187"
     CDS             7321..8172
                     /locus_tag="PWU31_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL1083744.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="PWU31_001188"
     CDS             8200..8835
                     /locus_tag="PWU31_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL1083745.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="PWU31_001189"
     CDS             8855..9628
                     /locus_tag="PWU31_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1083746.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="PWU31_001190"
     CDS             10036..10833
                     /locus_tag="PWU31_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL1083747.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="PWU31_001191"
     CDS             10811..11224
                     /locus_tag="PWU31_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1083748.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="PWU31_001192"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="PWU31_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1083749.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="PWU31_001193"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="PWU31_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL1083750.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="PWU31_001194"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="PWU31_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL1083751.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="PWU31_001195"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="PWU31_001195"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL1083752.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="PWU31_001196"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="PWU31_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL1083753.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="PWU31_001197"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="PWU31_001197"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL1083754.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="PWU31_001198"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="PWU31_001198"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL1083755.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNEKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="PWU31_001199"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="PWU31_001199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL1083756.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="PWU31_001200"
     CDS             21908..22486
                     /locus_tag="PWU31_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1083757.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="PWU31_001201"
     CDS             22538..23020
                     /locus_tag="PWU31_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL1083758.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="PWU31_001202"
     CDS             23137..23511
                     /locus_tag="PWU31_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083759.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="PWU31_001203"
     CDS             complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="PWU31_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1083760.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVQAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25688..26278
                     /locus_tag="PWU31_001204"
     CDS             25688..26278
                     /locus_tag="PWU31_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL1083761.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26406..27755)
                     /locus_tag="PWU31_001205"
     CDS             complement(26406..27755)
                     /locus_tag="PWU31_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1083762.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27923..28663)
                     /locus_tag="PWU31_001206"
     CDS             complement(27923..28663)
                     /locus_tag="PWU31_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1083763.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28676..30268)
                     /locus_tag="PWU31_001207"
     CDS             complement(28676..30268)
                     /locus_tag="PWU31_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1083764.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30836..31132)
                     /locus_tag="PWU31_001208"
     CDS             complement(30836..31132)
                     /locus_tag="PWU31_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083765.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31308..32033
                     /locus_tag="PWU31_001209"
     CDS             31308..32033
                     /locus_tag="PWU31_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL1083766.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32026..32868
                     /locus_tag="PWU31_001210"
     CDS             32026..32868
                     /locus_tag="PWU31_001210"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL1083767.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32871..33392
                     /locus_tag="PWU31_001211"
     CDS             32871..33392
                     /locus_tag="PWU31_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL1083768.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33645..34208)
                     /locus_tag="PWU31_001212"
     CDS             complement(33645..34208)
                     /locus_tag="PWU31_001212"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1083769.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34459..34728
                     /gene="rpsO"
                     /locus_tag="PWU31_001213"
     CDS             34459..34728
                     /gene="rpsO"
                     /locus_tag="PWU31_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL1083770.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34916..37042
                     /gene="pnp"
                     /locus_tag="PWU31_001214"
     CDS             34916..37042
                     /gene="pnp"
                     /locus_tag="PWU31_001214"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL1083771.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37552..37818
                     /locus_tag="PWU31_001215"
     CDS             37552..37818
                     /locus_tag="PWU31_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1083772.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37995..39953
                     /locus_tag="PWU31_001216"
     CDS             37995..39953
                     /locus_tag="PWU31_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL1083773.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40307..41608
                     /locus_tag="PWU31_001217"
     CDS             40307..41608
                     /locus_tag="PWU31_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1083774.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41738..43108)
                     /locus_tag="PWU31_001218"
     CDS             complement(41738..43108)
                     /locus_tag="PWU31_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL1083775.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43290..43613)
                     /locus_tag="PWU31_001219"
     CDS             complement(43290..43613)
                     /locus_tag="PWU31_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL1083776.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43938..44546)
                     /locus_tag="PWU31_001220"
     CDS             complement(43938..44546)
                     /locus_tag="PWU31_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL1083777.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44876..45592
                     /locus_tag="PWU31_001221"
     CDS             44876..45592
                     /locus_tag="PWU31_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL1083778.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45605..46300
                     /locus_tag="PWU31_001222"
     CDS             45605..46300
                     /locus_tag="PWU31_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL1083779.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46384..47013
                     /gene="udk"
                     /locus_tag="PWU31_001223"
     CDS             46384..47013
                     /gene="udk"
                     /locus_tag="PWU31_001223"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL1083780.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15392 a         8788 c         9877 g        13414 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagttcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacaac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatgaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaatg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcaat
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gacgaaaata aaataacaaa caatttgtaa agttgacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaaatttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcgaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacgat tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttttgcgaa aactattttt atatcatata tatgtctaaa atctatatta
    23881 taaaagaaat gttgtttgat cagcttctct tcggtattag ttcaaaacaa aacggcaaag
    23941 tatatggagc tactttgccg ttttgtttct ctaatactca ggtcagttta tagacgctca
    24001 ataaacggtg ttcaaaagcc agtttctcgg tattaagtca aatttatgca aaaatgtgag
    24061 aagccatttt caaaaaatat ttcttaatac tcgaaactga gcggcttttt cacgacctct
    24121 caataaacag tgttcaagag acagcctatc ggcaataagg ctgaacgtct ctttcacaac
    24181 ctcttttatc aatctgctac ttcttctttc ttagcgaaac tccaaatcag tccgataaca
    24241 aaaccgataa ctgctggtac gatccacccc atccccaatt tgaaaaacgg caagtagctt
    24301 tccgcaacat ataaaagatt ttgaacaata ggtgtttgtt tgatagtttc tgggcaagcg
    24361 ttcaacccat caaaaatcga agcaatcaat gtaaaataag tagtcattcg ataaacagcc
    24421 gtacgctgct taaacaaagg accaactaac actagtaaaa tcagtgtcat tgctaatgga
    24481 tagatgaaca tcaaaacagg caacgaaaat tgaatgatat tcgtcaagcc tacgtttgcc
    24541 gctaaacaag gaaggatact agcaagtgca acaaagaatg cgtaatttct ttgcggaaac
    24601 aaatctgtga atgtttctgc aaaagctgta atcaaaccaa taccggtctt caagcaagct
    24661 aatataacga taaatgctaa taagatactg ccataagtac ctaaataatg atcagcaatc
    24721 tgagccagcg tgatcccgcc attttcactt agagcaaagc tacctaagct catagcacct
    24781 aaataagcga gcaaagtata aatgattccc atcaatataa tgctgatcgc accagatttg
    24841 atcgtgtctt tagcgatttc agcaggtttt gttacaccca tgttttggat tgttgtgaca
    24901 atgatgatcc caaatgctaa agcagcaagc gcatctaatg tgttatatcc ttgagtaaac
    24961 cctgtgaaaa aagcatgctc ttgataattt ggctgaatag gcgcttgatt tacagaacct
    25021 agtggattca aaaaagcaag gagaagcaaa gcacctaata aaaccaaaaa tgcaggattc
    25081 aggaactttc caacataatc taatatcttt gtcggttttc ttgaaaacca ccaagcctgc
    25141 acaaaaaata caatactgaa aattgctaga tatagcgtct gtctgctagg atcgataaat
    25201 ggtgctaaac caatctcgaa agatgtggtt gcaaggcgag gtaaagcaaa aaacggtccg
    25261 ataactaagt aaagcaagat ggtaaaaacc atcgcatatg tttgatttat ccgttttgct
    25321 aattcatata cacctgagct tttcgataca ccaatggcaa tcacccctaa gaagggaaga
    25381 ccgataccgg taaccaagaa acctagattc gcccaaaagg tagcagcgcc tgcttcttga
    25441 cccaaatgaa cgggaaagat aagatttcca gcaccaaaaa atagaccgaa aagcatggag
    25501 cctataaaaa gatagtcttt aaatgataat ttttttgtca tacataactc ctttacggtt
    25561 tttttaaaag taacaaaatt tcgaaaaata tgcaacaatt ttcagaaaat ttcctatata
    25621 aaacgacggt tatttaataa cgtaagtgat aaaataaaag cgctgaatca tctgaggagg
    25681 aatcatcgtg acaaagtcga gattagaagc atttactgat ggagtggtag ccattgtttt
    25741 gagagttctt gtattagaga tcaagatccc taatacatct agctttcatt ctttatgggt
    25801 catccgaaat acattattag cttataccat cagctttata tttgttgcgg tgatctgggt
    25861 gaaccatcat cgtatatttc agatggcaga acgaattaat taccgagtgg tctggtcgaa
    25921 tatctttttg ctcttttggc tgactttatg tccttctgtt actagttggg tcgggcgtaa
    25981 ccctgaagct ttttggcctg agatggctta tgtcttagta tacacgatgt ggagtttttc
    26041 ttttggtatc ttgatgaggc aaatcatgaa agcaaataaa ccagattccc atgtcgtgag
    26101 agttttgtca aaggaccgtc gaagtcttat ctccatgatg attaacttat gcttgattgc
    26161 cggtgtcttt tttgtaccgt tgatcgggtt attcggacgt ttttttgtat ctggtatttg
    26221 gattttttcc tataaaaaag cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa
    26281 aagaggctgg gacagaagct tttaactcca agaaataagc cgaaatttca caaaaattta
    26341 agaagcaatt ttcgtaaatt ccttcttatt tctcgaagct gaccacttct gtcacaacta
    26401 ctttcttact tcttacgttc atatttcata tgatagatta gtaatagaag caagaaccac
    26461 acaggtgtta gtaataaagc gattctcgta tcttttccta ggaacaagac gacacacaca
    26521 aaggccatgt aaagaaatac cagataatta ctaaatgggt acaacggcat tttaaatgaa
    26581 tgagcttttt tgccgaggat acttttacga tatttcatgt gcgtgtagac aagcatactc
    26641 cagataaaca agaaacaagt agtggcaata ctgcttacta gtgtgaatac ttcactaggc
    26701 atcacgtaat tcaagataac agaaatgaaa atgacgatag tagaaaatgt caaagcattc
    26761 cctggcacat gatgtgttgt taactttttg aattttttag gggcactgcc ttcttgcgct
    26821 aaagaacgaa gcatacgtcc tgtactatat attgcactgt tgcaggcaga agctgctgca
    26881 cttaatacga ctaaattgat caaggaagca gcaatcgtga tcccaatttc cgaaaatact
    26941 tctacaaatg gactgttatc tggattcaag ctgttccatg gatagattgc cataatgaca
    27001 aaaagagatc ctaagtaaaa taagatgatc ctgataggaa tattattgat agcttccggc
    27061 aacacttttt caggattttc tgtttctcca gcgactaatc caactaattc tatcccagca
    27121 aaagcaaagg ttaccatttg gaaagctaag ataaatcctt ttgcaccagt tggaaagaat
    27181 ccgccatgag accatagatt cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg
    27241 aggatcatat aagctcctac gaggataaag gcaatgatcg ctacaacttt gatcaatgca
    27301 aaccaaaact ctaactcacc aaacaatgag acagccacca tattcaagca aagaagaaga
    27361 cctaatgcaa tcagttcagg aagccactga ggaacaccag gcgcccagta gcgtacatac
    27421 attccgatgg ctgttaaatc ggccattgca atcgataccc agcagaacca atacgtccag
    27481 ccagtgatga aagccgcttt tttgccaaga tattctgcaa caaaatccag aaaagaatgg
    27541 ttattggtgt ttgataacag aagttcgcct aacgcacgca taatgaaaaa acagatgcat
    27601 ccagtaataa gataggcgag caatatcgaa gggccagcta gctggataga ctttccggaa
    27661 cctaaaaaca accccgttcc aatcgctccg ccaatagaaa tcagttggac atgtctattt
    27721 ttcagtccac gctgcagttc ttgctcttca ttcattttta aatcatccta actaaaaaag
    27781 tttaattact tccatattaa aagagttttc tgaaaaacac aactaagatt ccaaaatttt
    27841 cagaaattta ccttttatga gtaattcata aaaaagaact gcggtaaaca ccgcagctct
    27901 ttgtcccaat cagctattga tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac
    27961 gttcttcttt cggatggaca aagatatctt ctggtgtacc ttcttctgca atcacacctt
    28021 tatccataaa aatgacacgg tcagatacat cttttgcaaa ttccatctca tgcgtcacga
    28081 taagcattgt caagccagta tgtgctaagt ctttgatcgt attaagcact tcgccaacca
    28141 tttcagggtc taacgccgaa gtaggttcgt cgaatagcaa tacatctgga ttcattgata
    28201 atgcccgagc aattgcgaca cgctgtttct gtccaccaga taattgtgct ggtttggctt
    28261 cgatataacg atccatacca actttttcca gattttcgat tgctactttt ttggcagttt
    28321 ctttatctcg tttcaggaca gttgtttgac cagacataca gttttccaag acattcatat
    28381 tgttgaacag gttgaaggat tggaaaacca tacctagatt cgttcgatat ttaggtaaat
    28441 tatatttagg agctaataca ttttctccat tataaatgat ctcgccgcct gttggttttt
    28501 ccagtaaatt gatacagcgc aagaaagtag atttaccaga accggaagaa ccgatgatcg
    28561 tcacaacttc acctttattg actgttacgc tgatatcttt caatacttca ttttcaccga
    28621 agctttttct tagatgtttg atttcaataa ttttttcatt catgattctt cctccttatt
    28681 cctgcaaatt tgaattgtcg atttcttcaa gcttggtata agctgaagga ccatcgattt
    28741 tcttttctac cgcccgcagg attctggtga tcgtaaaggt catgactaag tagatgatcc
    28801 cgacgatcgt aaatgtttgg aagaattgga aattcgcacc ggaagcagag tttccttgga
    28861 agaacaaatc agctacgccg attacactta aaactgctgt atctttgata ttgataacaa
    28921 actcatttcc tgttgctggc aagatattcc gtaaaacttg tggaataaca acttttctca
    28981 tcgtttggcc atgggtcata ccaatggctt gcgcagcttc gaactgccct tcatctactg
    29041 caaaaatccc cccacgaacg atctctgtca tgtaagctcc agtattgaga gagacaataa
    29101 acaaagcggc aatcgttcgg ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga
    29161 tgaccattgc ttgtaccatc atcggtgttc cgcggaatac ttcgacatag atagaaagaa
    29221 gtgcatttcc gattttttgg aagaagcgag tcagtgcatt tcctgattca ggtaccgaac
    29281 ggaagacacc aatcagtaat ccaagtgtag taccgacaac tgtcccgatc aatgcgatga
    29341 acaaggtaag tccagcacct cgtaagaaca tattgccgta ttgtttccaa atcgtagtga
    29401 aatcagaaat gatgccaggt ttttcttcac tatcagttgc agcaggctga tctttgactg
    29461 cttggtccat aattttggtt cgttcatcgt tagagatgcc agaaagaatc tggtttactt
    29521 tttggatatc cgggtcgcct ttacgcatac caacagaaat ctgaacatct tccgggtttg
    29581 tctggaagcc atctgcaccg ctgaattcaa gcatttccaa gtctttgttc acactagttg
    29641 ccgttacacc ttccggacgt tcgctgacat aaccatcgat cattccagaa gctaaagctg
    29701 ttctcattgc tgagaaatta tccattgctt gttgtttctg cacatctggt atctgatcga
    29761 tcacagtata gtggaatgta tttaactgag cagtaatctt tgctccagaa agatctgcta
    29821 aactttttgc ttttgcatat ttgctatctt tttgaactac tactactaat tgtgattcgt
    29881 aataaggatc ggtaaaatca atttcttttc gtcgttcagc agtagggctc attccggcga
    29941 tgatcgcgtc gatcttgcct gattgaaggg caggagccag accatcccat ttcgtttgga
    30001 cgatgactaa ttttttccct aaaccatctg ctactttttt agcgatttga acatcataac
    30061 cgccggcgta actattttgg ccttgaattg gcaaggcatt attctcatct gtttgttgag
    30121 accagttgaa gggtgcatac ccagcctcca tacctacgcg aaactcatcg ctcggtgttt
    30181 tttcatctgc atttgccaga gatcccacac tgaatagtga gaagacaaaa gcaaatagta
    30241 cggcaactga aaccatattt tttttcattt ttttctccct ttctgattat atgatacacg
    30301 agcggtctag atgaaatgcg gtaaagcttt atctgaaaaa ataaaaagcg accgatttat
    30361 agatataaaa aggccgctaa aattccataa ggtgataaac ctctcacaaa acatagcgca
    30421 acttagcatc actgctaaga cagtccgcaa gctcttaact tgcgtcccaa catacactcc
    30481 aagcaaaaaa gtgcacattt cggcgatcct ccctattcct gcttctccat gtttgcttca
    30541 ctccacaggg ttaatgattc tcgcgacctc tacctcattg attgaggcat ctcgtattca
    30601 gttatttata caaaaaaagt ttacgatact cgagaggctt tgtcaatcta taactgctgt
    30661 tttttgaaaa aatactgata aatatagaaa tctttacttt taccgaaaaa acaatcaaaa
    30721 tgtgaatcat ctatccattg caaacgatag tataattata cacaaaaatg tatgtaatac
    30781 aataaaaaaa gaactagaac agaaaatttc ctgtgtccag ttccatctcg atcatttaag
    30841 ctgccgcttt ctttggctca cgtcctagga tcgcttgcaa aagaacaacg acgccataaa
    30901 tggctaatat tagataaatc aaacttgagt attgggtgat tccccagccc catgttcgat
    30961 atacggctaa tgtaatggct aaaacaattg cggcaatcac gttgaataaa gcaaaagccc
    31021 aaagatttcc attttctttg cgtacaagat aaaaaacaga gaaataaacg cttaacgcta
    31081 accaggcgat acaagccaga ataacgcccc aataaataaa gaatggtaac atatcgattc
    31141 acctcacttt tatacagcag tattgtaaca cgttttcatt cttttgtcag tgaacattgt
    31201 gataaattgt tcaaatgccc ataacacttt tttattccca tttttctgac ttttcaacaa
    31261 taaatttgtt cgagtatggg ctataatgga aagtgaggtg acaaaacatg aaacaattaa
    31321 cagtagacga agcagcaaaa gagatattag cttatcagca aattacattt ttgaccggtg
    31381 caggggtatc cacaccttct ggagtgccag actatcgatc tttgtcagga atctaccacg
    31441 ggatggagca gccggaatac ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat
    31501 tctatcaatt tgtcaaaaag atttatcatc ctgaagcaaa acccaatatc attcatttgg
    31561 aaatggcaca tctagcgaaa gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt
    31621 tgcacgcaaa ggctaaaagc ccgcaactcg tcaattttca tggaagtttg tatcaatgtc
    31681 attgtcgaaa atgtcaacaa acggtagact ggaaagaata tcttcacagc gacaaacatc
    31741 aagcatgcgg aggacaaata cgcccagaca tcgtgttata tggtgaaggg tttcaagatg
    31801 gagtcatgga gcaggcggct tatgccgtca gccaagcaga gctgatcgtc attgttggaa
    31861 cgagttttca agttcatccg ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa
    31921 tcttagtaat caatcaaacg cctgtttatt tagcggagcc ttatacgttt attcaagaag
    31981 acggagcggt tgttttcaaa aaaatacaag aattaggagc agattatgac tgaattcaaa
    32041 accgaaaaag aaaaaatgat tgcaggcgag ctttattttg ccaatgatcc tgagctggtt
    32101 gccgaccgta tgtttgcaag aagtcaaagc caaatcatca accaagcaga atcagcggaa
    32161 ctgcgatccc aattgttgaa agagactttt ggccgtacag ggaaaaaaat ctatatggag
    32221 ccagttatca attttgatta cggatataat atatttgttg gggagaattt ttatgcaaat
    32281 ttcaattgta catttttaga tgtcagcacg attgaaatcg gtgataactg catgttcgct
    32341 ccgaatgtcc agctttacac ggcgacgcat ccactacatc cagtgaaacg taacagcggt
    32401 ctggagtacg caaaacccat taaaatcggt aataatgttt ggttaggcgg aggagttatc
    32461 gtcacgccgg gtgtcactct aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc
    32521 aaatcttttc ccgacaatgt agtgattgca ggaaatcctg ctcgtatcat caagacagta
    32581 gaggaagaac taacagaaga gtctcttgaa acattacgtc ataagatcga tatgatcgat
    32641 cgagagttga ttgctttatt agaaaaaaga atggatacgg taactaaaat aggacaagtg
    32701 aaaaaagaag aaaaccaagc agtttatgat gcaaaaagag agcagcaagt attggataaa
    32761 gttgttagtc tgctgaaaaa taagggatat aaggaaacaa taacagatac gtatgttgat
    32821 ttgatgaaac actcgcgcga ataccaaaac aaaatgaagg aggaataagt gtgatcgaag
    32881 gagagacacg gcgaaaacaa atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg
    32941 ctagcaagtt tgcgaaaact tttggagtca gccgtcagat cattgtaggc gatattgctt
    33001 tactacgagc tgcaggtaaa agtatcgtgg caacagcaag aggttatatc ctcgaaacag
    33061 aagaaaccaa tactgggcac atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag
    33121 aagaagaact gagactaatc gttgaaaatg gtggtgaaat cattgatgta attgtggaac
    33181 atccccttta cggtgagtta gcaggaactt tgcacatcaa aacgccacaa gatatcgatt
    33241 cctttatgag acgttatcaa aaaagtaatg ctactttgtt atccgaatta acaggcggca
    33301 tccatctgca tacgatccgc tacccagaaa agcatatgct gaaaaacatc aaacaaaaac
    33361 tagctgaagc aggaatcctc tacgaaggat aacatcgtag ctgctacttt ttatcctaga
    33421 aactattcta tcatctctga gataaacacc catatggaag aaataagaaa aaataagaaa
    33481 agatgcgtat ccaatacaca gaattttttg gaattgcgat ttctagatgt ctatctaaat
    33541 ttcgattagt ggagaaacat aaaaaaggga agtgcagctt ttgagcactc ccctttttta
    33601 taaagctttc ttgtagcata gtatcgaagg aacctgccaa ttctctactc gatcaccaat
    33661 acgccttctt ttagtgcaaa tggattttga tcgttgatgt ggtcatagaa catcacaccg
    33721 ttgatatggt cgatttcatg ttgaacgacg atcgattcat aattcttcaa acgaatcttg
    33781 tgtttttcac cattcatatc ataataagac acagtgattt tggcatggcg aacaacatat
    33841 cctggtactt ctcgatcaac agataaacag ccttcacctt cccctaaaca agcatcttga
    33901 acagaatggc tcaaaatttt aggattatac ataacagtac ttagagaagg tccatctgcc
    33961 tctgggtcag gacttggtac gtgaacagca atgattcttt tagaaatatc taattgagga
    34021 gcagctaagc caacaccgcc tcgtaagtgt agttcttcag ctttgattgg atcttggctg
    34081 ttttttaaga actcaagcat ctcttttcct aaagagatat cttcttcgct taatggaaga
    34141 gaaacttctt ttgcaacttc gcgtaaggtc ggatttcctt cacgaatgat atcatccatt
    34201 gtaatcatgg aatatgtacc cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt
    34261 atagtttacc acaaaaatgg aaaagtagta gtaagattgt ttgaaaacta ccttttctta
    34321 atgattaact acttgatttt caacccggtt tcaggtaaag tatagctgtg tgctctaggc
    34381 acatgacctg ccacttggtt aagcgaatca ccaacgtttt actcagtcgc aggggaataa
    34441 tcgaaagagg tgaaagaaat ggcaatttca aaagaacgca agaatgaaat catcaacgaa
    34501 tacgctcgcc atgaagggga tactggttct ccagaagtac aaatcgctgt attaactgaa
    34561 gaaatcaacc acttgaatga acatgcacgc gtgcacaaaa aagaccatca ttcataccgc
    34621 ggcctaatga aaaaagttgg tcaccgccgt aacttgttag cttacttacg taaaactgac
    34681 gttcaacgtt accgcgaatt gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa
    34741 agagtgggat tcacatgaat ctcacttttt ttactcgtat atcaattaag taaaattccg
    34801 tgatcaagct agatggactc ctactaacca aatgaaagct gctcaaaaga cggctttcgg
    34861 cagttttcat ttgtttagta gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc
    34921 agaaaaacaa gtattcaaaa cgacatgggg cggccgtccc ttacaaatcg aagtcggtca
    34981 gctagcaaaa caagcaaacg gagccgtttt agtccgttat ggagatacag tagtcttaag
    35041 tgcagctgtg gcctctaaag aagcaaaaga tacagacttt ttcccattaa cgatcaatta
    35101 tgaagaaaaa atgtatgcag cagggaaaat ccctggaggt ttcatcaaac gagaaggccg
    35161 tccaagtacg gaagcaacat tgactgcccg tttgatcgac cgtccaattc gcccgatgtt
    35221 tgcagaaggc ttccgtaatg aagtccaagt aacgaacatc gtgatgagtg tagaaacaga
    35281 ctgctcgcca gctatggcag caatgctagg ttcttcttta gcactatcaa tctcagatat
    35341 cccatttgac ggcccaatcg caggcgtaga agttggtcgt gtaaatggcg agtacgtctt
    35401 gaatccaaca gtagaacaag cagaacaaac agatatcgaa ttgactgttg ccggaacgaa
    35461 acaagccatc aatatggttg aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg
    35521 tgcattgctg tttggttttg acgctatcaa agagttagtt gctttccaag aagaaatcgt
    35581 tcaagcagta gggaaagaaa aaatggaagt gactttactg caagttgatg aagtattgaa
    35641 aaaagaaatc tttgatgctt cttacgctac aatgaaagca gcagtaatga ctgaagaaaa
    35701 acttgctcgc gaagataata tcgagcaggt gaagatcgat atccgcgaag cttatgctga
    35761 gaaatttgca ggtcatgaag atgaagatca cttgttgaaa gaagttaaac aaatcacaga
    35821 agatcttgaa aaagatgtcg tacgtgaatt gatcaccatc gataagatcc gtcctgacgg
    35881 gcgtaaattg gatgaaatcc gtccattgtc atcagaggtc agcttattgc cacgtgtaca
    35941 tggatcaggt ttgttcacac gaggacaaac acaagcttta tcagcttgta cactcgcacc
    36001 tctaggagag caccaaatca ttgatggttt aggggtggaa gtcagcaaac gattcatcca
    36061 ccactacaat tttcctcaat tttctgttgg ttctacagga cgtgcaggct ctccagggcg
    36121 tcgggaaatc ggacatggcg cattaggtga acgtgcgtta gcacaagtaa tcccttcaga
    36181 agaagaattt ccttatacga tccgtttagt agcagaagta ttggaatcaa atggttcttc
    36241 ttcccaagca agtatctgtg ctggcacatt agcattgatg gatgcaggtg taccaatcaa
    36301 agcgccagtc gccggtattg cgatgggact tgtttctgat ggtgaaaact atacgatcct
    36361 aactgatatc caagggctgg aagatcatct tggcgatatg gactttaaag ttgccggtac
    36421 aaaagatggt atcactgctt tacagatgga tatcaagatt caaggaatca cagaacaaat
    36481 cctaactgaa gcattaactc aagcaaaaca agcacgtatg gaaatcttag aagaattgac
    36541 ttcaacgatt gctgctccac gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat
    36601 ccagatcgaa cctgctaaaa tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg
    36661 aatcatcgat gaaactggtg tgaaaatcga tatcgatcaa gatggaaaag taagtattgc
    36721 ttcagctgat gcagaaatga tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga
    36781 agtcaaagtt ggtgaagttt atctagggaa agttgtccgt atcgaaaaat tcggtgcatt
    36841 cgtcaacttg atcaaaggaa aagatggact tgtccatatt tcccaattag caaacgatcg
    36901 tgtcaataaa gtcgaagatg ttgttaaact aggtgatgag atccttgtga aagtaactga
    36961 aatcgataaa caaggacgcg taaatctgtc aagaaaagca atgttgaacg aagatggttc
    37021 aacgaaaaat gaagaaaaat aatttaattc ataggaataa tgtacttgtg atcgtgaagg
    37081 tgacaggggg acaataatta ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc
    37141 cttttttttc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag
    37201 ttaaaaacat taggagctgt ttttatttgg ctgttcttat tacttgatgc agaacagctt
    37261 ttttacaacc tctccaataa acgatgttca atcagctgtc cctcgatatt agctcaaaaa
    37321 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaccgct
    37381 gatttcacaa cctctgattc acggtgttcc agaagcagct tctacggaaa taagctgaac
    37441 acttttgtta caacttcttt ttttctggag tttcttggcg catatttttg gagtccttct
    37501 gaataaagcg taaaataaaa gtgagaaata tggataaagg agggaaagga gatgctgaat
    37561 tttttatggt cgttatttat cggcggtata ttaggtttga tagctggcgc aattttagga
    37621 aaggaagtcc cgggaggcgt atctggaaat atcattatcg gttttttagg tagttggctc
    37681 ggcgaatttt tactaggacc actgggaccg gaactcggcg gtttttattt gataccagca
    37741 ttattgggtg caattttttg ccttgctctt tattcatttg tggcaagacg tatcagagaa
    37801 cgtccaaaga aagaataatt ttattcaaat aagttgacac ttcagtgtgt tacttggata
    37861 attagaagca atttaggaaa gcgggtgtca tggataagaa tatcatctat cattcaagct
    37921 tacctaaagg atatccatat aacaagaaat gagggaaaaa cgtggcaaca acgcacaata
    37981 ccaaaaagat atcaatggct ggcctgcttg tagcgatggg agtcgtctac ggagacatcg
    38041 ggacaagtcc gctttacgtt atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa
    38101 tcaatcctga ttttgttatc ggatcagtct cattgatttt ttggacattg actctactga
    38161 caacaatcaa atatgtcgtg attgcattga atgcagataa ccatggtgaa ggtggaattt
    38221 tctcactcta cacacttgtc cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg
    38281 gaggagctgc cttactagca gatggggtat tgacccctgc tgtgacggta acgactgcga
    38341 ttgaaggctt gcgaggaatt ccgcagtttt ttgaccggtt cggtaataac cagaacgtga
    38401 tcgtcattat cactttagtt attatttttg ttttattcat ggtccaacgc tttggtacag
    38461 atattgtcgg aaaagcattc ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa
    38521 tgggattaat gaacttcagt caagactgga cagttatccg tgcactgaat ccttattatg
    38581 ctatccagct gctttttagt gcagataaca aattaggctt gttcattctt ggaaatattt
    38641 tcctggctac gacaggtgca gaggcgcttt attccgatct tggacatgtc ggcaaacgaa
    38701 atattcgtct gagctggcct tacgtcaaaa tttgtttggt tatcaattat tttgggcagg
    38761 ctgcgtggtt gttgaatgtt tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc
    38821 cattcttcca aatgatgcct caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg
    38881 ctgcagtcat tgcttcgcaa gcattgatta caggttcgtt tacgctagtt tcagaagcaa
    38941 tcaagttgaa actgctgcct cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa
    39001 tgtatatccc agcagtcaat ctgattttat ggatcgcctg ttctctagtg gttgtcacat
    39061 tccgaacgtc tcatcatatg gaagctgctt acggtctatc gatcactgtg acgatgttga
    39121 tgacaacagc actgctttac ttttatctta tccaaaatgg gcattctcgt tggcttgcat
    39181 atttagttac ctttttcttc ggtgcgatag aagtcgtttt ctttatctca agtgttgtca
    39241 aattcttccg aggcggtttt gtagccgttc tgattggttt gatcatctta gtcgtgatgt
    39301 ttatttggga acaagggaac attattcgcg aatcgtcaac cgaggatgtg tctttgagag
    39361 attatatccc gcagcttaga gaattacgtg atgatacttc tttaccaatc tatcagacaa
    39421 atgtggtctt cttggttcct gatatggctg aagataaagt tggccgacag ttcatctatt
    39481 cgattttaga taaacggcct aaaagagcac gagtctattg gtttgttcat gtggaagtga
    39541 cggatgagcc atatacgaaa gaatatcaag tagatatgat ggacactgat tttattgtcc
    39601 aagtgaatct gttcttaggt ttcagggtcc aacaggaaat caacgtatat gttcgccaag
    39661 tcatccatga tttgatgaaa caaggccgtt tgccaaaaca gccacaaaga tactctctga
    39721 caccagggcg agaagttggc gatttccaat ttgtcctgat tgatgaagta gtttccaatg
    39781 tgacaactct aggaaaatgg caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa
    39841 tcgctacctc gccagaaaca tggtttgggc tagagtacag cgaagtaaaa catgaaactg
    39901 ttccgcttat cattggaaat actcggaaaa catggttgaa agagcggttg tgaaagtagc
    39961 gctttgacct gaactaatgt cgaatgtcag ctacttgaac accgtttatc agaggtcatg
    40021 aaaaagctgt actgcaacaa gcagtaagaa cgagcagata aaaacagctt ctcatgtttt
    40081 tcatctgttt gggcttaatg ccgtagatgc aagcttttga ataccgtttt tatcagaggt
    40141 catgaaaaag ctgtttagca acaagtaacg caatgatttt gaagtgataa ggagatgtgg
    40201 ctgaaaaatg ccgcatctcc ttttttattc ctaaatatga atacgcatta ttttcaatct
    40261 ttttgtagaa aaagggtaca atagaaaaag agaaaggcgg gagaatatgg ttatcaattt
    40321 cattatttta tttttgatga ttgcaattac ttcatttttt gtagcaagtg aatttgcttt
    40381 agtgaaaatc agacgatcaa gattggaaca actagaaaag gaaaatgtaa agaacgccaa
    40441 gctagctctt catgttacac accatttaga taactattta tcagctagtc aattaggaat
    40501 tacgctaacc ggtttgatca tcgggtgggt cggtgaagga tctgtcgcgg ctttactaga
    40561 accagtgatc ggtaaactac cttttagttc ggctattagc agtacaatct ctgtggcgct
    40621 gggatttatt ttagttacgt atatcgatgt ggttattggc gaactgcttc ctaagagcta
    40681 tagtatcgtc aatacggaga aggtcgtctt gttcgtagtt aaaccacttc attattttta
    40741 taaagtaatg tttcctttta tttgggtatt gaatcattca gctgcagggc tagggaagct
    40801 attaggtgtg cgtctcgtat ctgaaggaga agagacattg tctcaagaag agctgacatt
    40861 agtcgcattg aattcttatg aaaaaggaga gttgaccaaa gaagaatatt attatttgga
    40921 aaacgtgttt gaatttgatg ataccttggc aaaagatata caagtagacc gtacgtccat
    40981 gcaagtattc gaagcttcag aaaccgtcaa agaagcgata aaagcttcga ttgaacaagg
    41041 acatactcgc tatccggtta tcttagaatc aaaagacaat gttttaggtt atgtcacctt
    41101 gccagatttg atcaagcaat cttataaaga tgaccagctg acagtagaac agttgattga
    41161 agagccgatc gtaacaactg aaacgatccc tataaaaaaa ttattgacga tcatgcgaaa
    41221 aaagggtaag catattgcta tcttaaaaga tgaatacgga gggacaagcg gcttagtgac
    41281 gattgaagat attttagaag aaatcgtcgg ggaaatccga gatgagacag atttggacga
    41341 agcattgata gcggaacagt cagatggctc ttatatcatc tccggcaaac taacattaga
    41401 tgatttccag cgttattttc atgtagagat tccagagttt gaagaaacga attttactac
    41461 acttgcgggc tttgcttcta gccgatataa agaaataaaa gcaggaacaa tcattgagat
    41521 tgcatcattc cgtttcacgg tattagaata ccagcatgca catatcgatt atttcaaagt
    41581 agagtccacg gaaagaaaaa cagaataaaa gaaaaaaggc tgtgacattt tttgtcacag
    41641 cctttttgtt atctaaaaaa cctgttttcg gtaaacgaag aagatatttt caataaaagt
    41701 tttcattttt agccgcaacg atttctttta atcaaggcta tcttgcttct gcatgttcta
    41761 atgtgaaatg ttcctcttca cccatgtgtt tcaattgttt catcgaacgc atataagagt
    41821 aaagcaacac ggcaacagaa ccgatccaag ctaaaggaga agctgctgct gctcctgggt
    41881 atccccacat tcctgtaaga acgatagctg caaaagaacg cataagcaat tccatcatac
    41941 cggcgatcgt agggatcttg ctttgtccaa gcccttgaag tgtgtagcgt aaaatgaaca
    42001 ggactgctaa gatccagtaa agagacccat tgatattgaa gtaggtttgt gctaattcaa
    42061 atacacgtgt ttctgatggg ctaacaaaca attgaacaaa gaaatgaccg aagaaaatga
    42121 cgatagcacc tgctaagaga ctaaatccga cactcatcag taaagtttga cgtacccctt
    42181 gcaagattcg gccatattct ttcgcaccat agttttgtgc agtgaaagtt gccattgcaa
    42241 taccaaatga catcatcggc tgatttgcaa attggtcgat acgaccagca gcggcttgag
    42301 cagctacgac atctgtccct agactattta gtgctgcttg taacacgata gcaccaacag
    42361 cgatgatcga agattgaaac gccattggca aagcagcatt taggtgtaca gcaaactctt
    42421 ctttttcaaa tttgaaatct tttttgcgta actgtaacaa aggcattttt ttcttgatat
    42481 acactagaca cagtacgctg gaagaaactt gtgcgattac tgtagcaata gctgcacctt
    42541 ccacacccat gccaaaatag ataataaaga tcaaatcaag tacgacgttg atgatgaccg
    42601 caatgatcag aaagaatagt ggtgtgcggc tgtcaccaag tgcgcgaatg acattagaaa
    42661 gcaaattgaa actgactgat gcaaagatcc ctaataaaat aattgaaata aatgtttgtg
    42721 cttgatcaag tatttcagga ggagtttgca tcaattgaag catcggacga ataaacagga
    42781 gacttaggac agttaaaatc acagtgacga caagactaat gacaacacta gcagcaaaac
    42841 ttttcttcag accccgataa tcttttgcac cgtaacgttg tgcggtaata atggctaaac
    42901 cagctgttaa accttgggca aagccaatga tcaaaaatgt caggcttcca gttgcaccaa
    42961 ccgctgccag cgcattttta cctagcgtct gacccacgat gatcatatcg atcatattgt
    43021 agaactgctg aaaaacattt ccgatcagta aaggaacagt gaacatcaaa atcagtttag
    43081 cggggcttcc ttttgttaaa tcgcgcatct tttaaactcc ttatatcttt aaaatttagt
    43141 tagtagtata tagcgtttat cctgagaata ccaattagaa atcaatgata atttatgaaa
    43201 ctccataaaa agaagccaag aattgagtca aagaatcaac aacttggcct ggtctgtaaa
    43261 ataagggaca tccacgttta tctatcacct taatattgtc taatctcact tggtaaaagg
    43321 atcgtttctc tcgtaccgtc atcataggcg tatacttgag caggaaattg gttcgaatag
    43381 gcaaacggca aatcaatatc catttccaca ttgcttcctg cttgagaaaa atgcatcgtc
    43441 acttttccac gattttcgat caaatcaaag gttaatatat tttctaatgg aatgactccc
    43501 ttaagatcta ggtcaataat aaaccatata ctgtcaatca attcatctgg caaacttgat
    43561 acgacaccaa ctgaagcgta acggttgcgg ctgctatcaa aactttcaaa catctgttta
    43621 acctccttcg aatcaacgaa tgttttattt ttataattaa tcctaggata aactagaaat
    43681 acttacttca acgagggttc attctaataa agaatcagga aaaatgcaac aataattgaa
    43741 tacttttttt caaagtataa atttttataa aggtacgata aaagtatgcg aggaaaagct
    43801 tggtaaatta ggatttttcg tttgtaaagc catttacaaa agtggaagcc ccatatgaaa
    43861 ataaacgatt agaaataaaa aaggcgtgtt aaaagctgct gacccgccgc ttctaacacc
    43921 acctgattgt ttcagtatta ttcttctgtt gaaccgatca cttctggttc gccaccttca
    43981 cctgtgcttt cttcttcttc actttgagcg actacagcag cgatttgctc ttcactgtcg
    44041 gtcacgaatt tgtaatcgtc gttttttgga agatcggcta ctgtaataga atcacctaat
    44101 tccaagtttg tcacgtcaac ttcaattcgt tcaggcaatt tgtctggtgt agcagatacg
    44161 actactgtat acaagttttg agctaacact ccaccagatt tagcacccac tgattcgcca
    44221 acaagaacga cttctgtttc gacttctgtt tcttccgaca tgtttacaga tagaaattct
    44281 gcatgttcga aatgattggt aaaagtgttc gtttggatct tgtgaaccaa tgtattgact
    44341 ttttgtccat cgatgtccat cgtgatgact gtgttcgctc catgttcacg caacaatttg
    44401 cttagtgtca aaccatcgaa tgagataggg gtactttcta ctttataccc ataaacaaca
    44461 gctggaactt tcccttcgtg acgaagtttg tttcttaatg aacggggacg tacttctctt
    44521 ttacttactt ctaatgatac tgacataacc aaaattcctc cttaaaatgt gactgtctgc
    44581 tttggtaacg gacagatttt atttaagtga attctaaagg ctaatctcgt gcacatcatc
    44641 gaaggcaggg cgcttaaaaa agcgcaaaag gagagatgcg cgcatctccc cttagaaagt
    44701 caggattcaa cttttctcca ccaggtcatt acgctgtgta tgagaaatcc aattcacttt
    44761 actatttaac cgtaacgtga actgttaatt aagtcaagga ataactatga tgatgtattt
    44821 gcaagcaaga atattcaagg tataattaat tagtagtaat aagaaaggaa acagcatgcg
    44881 tttagataaa ttaatagaag aaaaattaga aacgacacgc aaggaaatga agcggctgtt
    44941 tgctttgaag caagtgatga tagatggcac agtcgaattt cgacaaaatc gaaatgtaga
    45001 tagtttactg catcaaatcg aagtagcagg tgaacactta gaaacaaatg agtcctatta
    45061 tcttctcaat aaacctgaaa aagctgttac agctgtatca gatccagaaa agacgacagt
    45121 aatcgatctg attgcaagtg ccgatcgttt ccaaccatta tatccagtgg gaagactaga
    45181 tagagataca acaggactat tgctgatcac ttcgaatgga caattgggat atgaaatgct
    45241 acttccagaa aaaaaggttt caaagactta tcaagtagtt gtgaacgaaa gggtgacacc
    45301 ggcagacgta acagcatttg aaaatggaat cgtattcgct gatgggtatc aatgtaagcc
    45361 ggctgattta aaaatactca aagcagacaa gcaccaaagt acggttttat tgacgatcca
    45421 agaaggaaaa ttccatcaag tcaaaaagat gttcttagcc caaggaaaga aagtgaccgc
    45481 attgaaacgg ctaagcatgg gaccattgga attagatgaa cagctaggag taggcgagta
    45541 tcgatcgttg acattagcag agatgaaaaa gttaagtatt tattttaaat aggaggtcaa
    45601 aaagatgaaa tataaaactt tattgtttga tgtagatgat acattgttag attttcaatt
    45661 aactgaagcc aatgctttaa gtgctttgtt tgcagaacaa ggaatcacat tgacaccaga
    45721 aatcgaaacc agctataagg ctttgaatca tcatttatgg caggagtttg aaaaaggcag
    45781 actatcacga gaagaaataa taggcagtcg ttttgggtta cttttccagc aatttgggaa
    45841 aacagtagat agtgatcaaa tggaaaaacg ctaccgccac tacttgaacc aaggtcatga
    45901 tcttgttcca ggaagtctag aaatcctgaa aaaggtccat ccgcatgctg atctttatat
    45961 tgtgacgaac ggtgtgtcgc acacgcaaca tcggcgatta acagatgcag gaatgcttga
    46021 ttacttcaag gatatctttg tctcagaagc tgtcggagcg caaaagccga tgaaagaatt
    46081 ttttgatcat gtcttttccc acattcctaa ttttgataaa aagcaaactg tgatcattgg
    46141 tgattctttg acttctgata tcaaaggagg aaatatggca gggatcgata cgatttggtt
    46201 caatagaaat aaattaccag aaatcgaaga aatccagcct acttatcgaa tcgattcatt
    46261 agaggaactt tattcattat tggaaattga aaaggattaa ttttcagaaa aatatagcat
    46321 tttcgtgttt tttcgctata atagattaga atgaaaaaac ggatacgaaa gaggttttag
    46381 tcaatgacaa aaagcaagcc aattatcatt ggtgtgacgg gtggatccgg aagcggaaag
    46441 acaagtgtca gccgtgcgat tttcaacaac ttcccggacc attccatcat gatgcttgaa
    46501 caagattctt attacaagga tcagagccat ttgagttttg aagaacgatt gaacacaaat
    46561 tacgatcacc catttgcctt tgataatgat ctgctgattc agcatgtagg cgacttactg
    46621 aactataaag ccattgaaaa acccgtgtat gactatgtag cgcatacaag aagtcaggca
    46681 acgatcattc aggaaccaaa agaagtgatc attctagaag gaatcctgat tttagaagac
    46741 gaacgtctgc gtgacttgat ggatatcaaa gtatacgtag atacagatga tgacattcgt
    46801 atcattcgtc gtatcaaacg ggatatggaa gaacggggac gcacattgga ctccgtgatc
    46861 gaacaatatt tgacagttgt taaaccgatg tatcatcaat ttatcgaacc aacgaagcgc
    46921 tatgcggata tcattgttcc agaaggcgga gaaaaccacg ttgctattga tttgattaca
    46981 acaaaagtag ctagtttctt aaaccataaa tagtgcgcag cgaaacagtt gaacggactg
    47041 cgaccgtttg aaaagcaagg attctgcttt ttgaacagtc accagccaac tgttttttta
    47101 tttataacaa gaggttgtga aaacagcatc ttgacctaag tattaaaaag aagaaacgga
    47161 aaatagctgt ttatatttta ccgttttttg agctaatacc gaaggtcagc tgattgagca
    47221 ccgtgaagta gaggttgtaa aaaagctgtc tttgcatcaa gcgataagag catccaaata
    47281 aaacagctcc tcacgttttt cgttgggttg ggcttattat cgtagatgca agcttttgaa
    47341 caccgtttaa cgagaggttg taacagaagt ggtcagatgc aataaataag aagaaactta
    47401 cgaaaattat ttctcgaatt tgtgtgagat ttcgacttat ttttttgaag aagctctttt
    47461 gtcgcacccc c
//
LOCUS       DANJMA010000019        46289 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000019 DANJMA010000000
VERSION     DANJMA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            547..1275
                     /locus_tag="PWU31_001224"
     CDS             547..1275
                     /locus_tag="PWU31_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1083781.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1293..2486
                     /locus_tag="PWU31_001225"
     CDS             1293..2486
                     /locus_tag="PWU31_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1083782.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2479..3474
                     /gene="lacD"
                     /locus_tag="PWU31_001226"
     CDS             2479..3474
                     /gene="lacD"
                     /locus_tag="PWU31_001226"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL1083783.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3471..4412
                     /locus_tag="PWU31_001227"
     CDS             3471..4412
                     /locus_tag="PWU31_001227"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL1083784.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4458..6224
                     /locus_tag="PWU31_001228"
     CDS             4458..6224
                     /locus_tag="PWU31_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDL1083785.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6241..6720
                     /locus_tag="PWU31_001229"
     CDS             6241..6720
                     /locus_tag="PWU31_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDL1083786.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6733..7623
                     /locus_tag="PWU31_001230"
     CDS             6733..7623
                     /locus_tag="PWU31_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1083787.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7610..8419
                     /locus_tag="PWU31_001231"
     CDS             7610..8419
                     /locus_tag="PWU31_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1083788.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8435..8839
                     /locus_tag="PWU31_001232"
     CDS             8435..8839
                     /locus_tag="PWU31_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1083789.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(9020..9958)
                     /gene="yidC"
                     /locus_tag="PWU31_001233"
     CDS             complement(9020..9958)
                     /gene="yidC"
                     /locus_tag="PWU31_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1083790.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10292..11374)
                     /locus_tag="PWU31_001234"
     CDS             complement(10292..11374)
                     /locus_tag="PWU31_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1083791.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11516..12967
                     /locus_tag="PWU31_001235"
     CDS             11516..12967
                     /locus_tag="PWU31_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL1083792.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12960..13682
                     /locus_tag="PWU31_001236"
     CDS             12960..13682
                     /locus_tag="PWU31_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1083793.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13702..14853
                     /locus_tag="PWU31_001237"
     CDS             13702..14853
                     /locus_tag="PWU31_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1083794.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15201..15476)
                     /locus_tag="PWU31_001238"
     CDS             complement(15201..15476)
                     /locus_tag="PWU31_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL1083795.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15584..16348
                     /locus_tag="PWU31_001239"
     CDS             15584..16348
                     /locus_tag="PWU31_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL1083796.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16472..16975
                     /locus_tag="PWU31_001240"
     CDS             16472..16975
                     /locus_tag="PWU31_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1083797.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17240..17887
                     /locus_tag="PWU31_001241"
     CDS             17240..17887
                     /locus_tag="PWU31_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL1083798.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            18048..19316
                     /locus_tag="PWU31_001242"
     CDS             18048..19316
                     /locus_tag="PWU31_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL1083799.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19493..20695)
                     /locus_tag="PWU31_001243"
     CDS             complement(19493..20695)
                     /locus_tag="PWU31_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1083800.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21222..21947
                     /locus_tag="PWU31_001244"
     CDS             21222..21947
                     /locus_tag="PWU31_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDL1083801.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21937..22611
                     /locus_tag="PWU31_001245"
     CDS             21937..22611
                     /locus_tag="PWU31_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDL1083802.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22899..23183)
                     /locus_tag="PWU31_001246"
     CDS             complement(22899..23183)
                     /locus_tag="PWU31_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HDL1083803.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23477..24784)
                     /locus_tag="PWU31_001247"
     CDS             complement(23477..24784)
                     /locus_tag="PWU31_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL1083804.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25273..26379
                     /locus_tag="PWU31_001248"
     CDS             25273..26379
                     /locus_tag="PWU31_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083805.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            26970..27170
                     /locus_tag="PWU31_001249"
     CDS             26970..27170
                     /locus_tag="PWU31_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1083806.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            27317..30106
                     /locus_tag="PWU31_001250"
     CDS             27317..30106
                     /locus_tag="PWU31_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1083807.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            30153..30680
                     /locus_tag="PWU31_001251"
     CDS             30153..30680
                     /locus_tag="PWU31_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL1083808.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            30701..31891
                     /locus_tag="PWU31_001252"
     CDS             30701..31891
                     /locus_tag="PWU31_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1083809.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            31931..33229
                     /gene="asnS"
                     /locus_tag="PWU31_001253"
     CDS             31931..33229
                     /gene="asnS"
                     /locus_tag="PWU31_001253"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL1083810.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            34163..34837
                     /locus_tag="PWU31_001254"
     CDS             34163..34837
                     /locus_tag="PWU31_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1083811.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            34834..35175
                     /locus_tag="PWU31_001255"
     CDS             34834..35175
                     /locus_tag="PWU31_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083812.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            35205..35564
                     /locus_tag="PWU31_001256"
     CDS             35205..35564
                     /locus_tag="PWU31_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL1083813.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            36088..38169
                     /gene="cadA"
                     /locus_tag="PWU31_001257"
     CDS             36088..38169
                     /gene="cadA"
                     /locus_tag="PWU31_001257"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1083814.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            38480..39946
                     /locus_tag="PWU31_001258"
     CDS             38480..39946
                     /locus_tag="PWU31_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1083815.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            40256..40729
                     /locus_tag="PWU31_001259"
     CDS             40256..40729
                     /locus_tag="PWU31_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDL1083816.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFIVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            40803..41243
                     /locus_tag="PWU31_001260"
     CDS             40803..41243
                     /locus_tag="PWU31_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL1083817.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            41256..41465
                     /gene="copZ"
                     /locus_tag="PWU31_001261"
     CDS             41256..41465
                     /gene="copZ"
                     /locus_tag="PWU31_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL1083818.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            41465..43651
                     /locus_tag="PWU31_001262"
     CDS             41465..43651
                     /locus_tag="PWU31_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL1083819.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            43671..45842
                     /gene="copB"
                     /locus_tag="PWU31_001263"
     CDS             43671..45842
                     /gene="copB"
                     /locus_tag="PWU31_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL1083820.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(45954..>46289)
                     /locus_tag="PWU31_001264"
     CDS             complement(45954..>46289)
                     /locus_tag="PWU31_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HDL1083821.1"
                     /translation="FRKSLRWTSYLIYLHFFASTNTIAYQKLLNPLETLVPRKPAESL
                     QEYSLRIEQEFKLDLRFIRLTTQYEAEIYGRQADPVKFDKKTYLEITRIIRKTVYKKI
                     LTKGSRFKK"
BASE COUNT        15139 a         8040 c         9834 g        13276 t
ORIGIN      
        1 taaagttcac aaacatcgaa aagtatagag cccgaatttt acgaatattt tttgtaatat
       61 aaaagtatag atactaaatt cttattcgtt aaacttacgc cataaggaaa ctagaaaaag
      121 acagaattat cataaaaaat ttttgttatt tttaaaaatg tacaacaaaa tttcgactat
      181 cagaccggca tgagagaaaa gttatctaaa aaataaaaaa tcctacttta tttagagaaa
      241 gccattttta tttacatagt agtctattag gcccatttgc ttgtcgatag tagaaatagc
      301 cacagatgta taaagaatat tttttcttta cttctctaga ccatttagct gaatctttgc
      361 caagattcag cttttttttg ttttttattt gtcaactggt atttttgagg tatcttttga
      421 taccagttct aaattacctt gaaaccttgt tataatagct tttttacgcc aaaaaccctt
      481 tgacaaatga ggaatatgtg ggtattcttg tgttgtggtt ataaccagta aagaagaggt
      541 atttttatgg agagctcaag taaaggaaaa tcattatatt atcaactagt tgacgtattg
      601 aaagaacgta tcgaaacaca gatgacacct catgacaaat tgttatctga aagagaactt
      661 tgtcagcatt atggagtgag tcggacaacg gttcgccttg ctttgcaaga attagagacg
      721 caagggtata tttatcgccg acacggaaaa ggaacatatg tgtctgatct atcagataag
      781 tcgtctgatc tggcgggagc gtatagtttt acagaagaga tgagggaatt gggacgggtg
      841 cctaatacag tcatcttgga attcaagcaa atggaagcaa acaaaaaggt tgccaagaat
      901 ctaaacatca atttaggcga aacagttttc aaattgaaaa gacttcgttt agcggatgac
      961 gaaccattga tggtggaacg ttcttattta cctgcaaaac tatttattgg tcttgatctg
     1021 ccattacttg aaagtaagcc attgtatgat gtgttttctc aggattatga acagcaagtc
     1081 caattagcag atgaagaatt ttatgcaagt atcgttcaaa agaaagatgc tgatccgctt
     1141 cgtatcgctg atggtgcccc ggtattatat ctaagccgaa cgacctataa tgtgaaaaat
     1201 gaaattattg aatatacgga tagtgtagcg agagcagacc agtttcatta taagatccgc
     1261 cacgtccgaa aatgattgag agggaaaaca acatgtttga actatcgaag gataaattaa
     1321 aagagttggg tgcggaaata actacgcgag aaatcaaaca gcaaccgcaa ttatggaaag
     1381 aaacagctga gatattagca cagaatcaag aaaaattagc attgtttttt gaccagataa
     1441 aaaaagcagc aaatggcaaa agagttcgag tgatttttac tggtgcaggc acatcacaat
     1501 atgtcgggga tacagcagta ccttatttaa atgaatacgg tgataacgat cactttattt
     1561 ttcatagctt tggtacgaca gatatcgtgt cttcaccaaa agaatttcta tttccggatg
     1621 aaccgacatt actggtgtca tttgcacgta gtggaaatag tccagaaagt gtggcaacag
     1681 tcaagattgc caatgaacaa atcaagacat tgtttcattt gacgattact tgtgctaaag
     1741 aaggtcagtt agcaaagaat gcagcgacag atgaacgaaa ttatctttta ctgatgccag
     1801 aaaaatcaaa tgatgcagga tttgccatga caggaagttt tacgtgcatg ttgttgagtg
     1861 cgttatgggt attcgaccaa actgccgttg agaaaaaaga aagttatctt caacaattga
     1921 tcagaactgg agaagatcta atttcacgag atcaagaaat ccaaaaatta ttgcctaagt
     1981 catttgaacg tatcgcctat cttggttcag gaagtttggc tggtgcaacg agagaagcac
     2041 aattgaaaat attggaattg acagccggta aagtggcaac gatctttgat tcttctatgg
     2101 gcttccgcca tggaccaaaa tcgtttttaa atagcgaaac acttgttatt ggttttgtta
     2161 ataaccaacc gtatacaaga aattatgact tagatattct agaagaaata aaagatgacc
     2221 aaattaccac gagtgtcatc gcgatcgccc aaccaggaaa catcgatttc acaggtacaa
     2281 gtcttttgtt agaagcttcg atcagcctac ctgatgcgta tcttgcctta cctatgatca
     2341 tgacagccca aatcattgcg ctcgatacgt cgataaaagt agcgaataaa ccagatacac
     2401 catcgcctac aggtacagtc aatcgagtgg tcaaaggtgt cattatccat gaatatgtga
     2461 acaaagggga aacgaaggat gaatgaagaa aaaattgcag ctatgaagag tttatcaaca
     2521 aaagaaggaa tcatcagtgc attggcaatt gatcaacgcg gtgctttgaa aaagatgatc
     2581 aaagcgttag gaaaagaacc aacggcgcaa cagatcactg aattcaaaga attagtatca
     2641 gcagaattaa cgccttatgc ttctgctatt ttactagatc cagagtatgg attgccagct
     2701 gctaaagcca gacatcagga agcaggacta cttttagcct atgaaaaaac gggttatgat
     2761 gcgacaactc caggacgtct gcctgattta ttagcggatt ggtctacgct tagattaaag
     2821 gaacaaacag ccgatgcgat caagtttttg ctttattatg atgtagatga agacccagag
     2881 atcaatcatt tgaaacatgt atttatcgaa cgtttaggca gtgaatgtgc aggtgaaaag
     2941 cgtccgtttt atctagaatt ggtttcgtat gatgccaaaa ccaatgacag cacttcatta
     3001 gaatatgcga agatcaaacc gcataaagtg atcgagatga tgaaagaatt ttctgatcct
     3061 aaatataaag tggatgtatt gaaagtagaa gtgccagtca atatgaacta tgtagaaggt
     3121 tattctgatg gtgaaattgc gtatagtaaa aaagaagcag cggcattctt caaggaacaa
     3181 agtgatgcaa caccgttacc atttatcttc ttgagtgcag gggtttcgac tgcattgttc
     3241 caacaaacat tgatctttgc tcatgaatct ggttcaacat tcaacggtgt gttatgcgga
     3301 cgtgcgactt ggaaagatgg agtcaaagca tatgttgaaa atggtagaga aggtgcgatt
     3361 gagtggttga gaacacaagg gcgacaaaat atcgaatcgt tgaataaagt gataaatgaa
     3421 aacgccaccc cttggtttga caaagtaaaa gaagattcgg tgacagcaaa atgatcacga
     3481 cagttaccat gaatccttca attgacatcg cttacacgtt ggatgaatta cggatcgacc
     3541 aagtcaatcg ggtcaaaacg gtcaacaaaa cggctggagg aaaaggcttg aatgttgctc
     3601 gtgtccttca tttattagca gatgatgttt tagctactgg attagtcggc gggcagcatg
     3661 gagcatatat caaagaacaa ctcgatcaat cagggataac gcatcaattt ttacctatca
     3721 aaggggaaac gagagattcg attgctattt tacatggagt gaatcaaaca gaactattgg
     3781 aaagcggtcc tgttgtgacg gaagaagagc aacataaatt taccgagcat tttgcacgac
     3841 taatggaaac aactgatatc tttacgattt cgggtagtct gcctcaagga ttttctaaaa
     3901 gttattatgc agagttactt gccctaacga ataaaaaagg aacacgagta ttgttagata
     3961 cttctggtca aaccttgctt gaagtcttgg aaggcaagga aaaaccattt ttgatcaaac
     4021 ctaatttgga agaattagga gagttggtcc accaaacaat ttcttcggct acttcgttag
     4081 atgaaatcca atcattattg gatcatccgc tttttgaagg aatcaattgg atcgttcttt
     4141 cgctaggcgc aaaaggagca atcgctaaag tagggagaca cttctatcag gttacgattc
     4201 ctaaaatcgc ggtggtgaat cctgtggggt caggagattc gactattgca ggcttagcac
     4261 atgctctatc aaagagctat acgattgaag aaacacttgc gtttgcgatg gcctgtggga
     4321 tggcgaatgc acaacaagaa aaaacaggat ggatcgatcc tgtgaaagtc gattatttta
     4381 ggggggaaat caacgtcaga aagattcgtt gattataaaa ttagaaagcg gtttctaaaa
     4441 acgaggagga aatttgcttg actagatttg aaataaaaga agagtttttg ttagacggtc
     4501 aacctttcaa aattttatct ggtgccatcc attatttccg agtgcatccg acgaattggg
     4561 agcggtcatt gcacaatttg aaagcgcttg gatttaatac tgtggaaacc tatgtgcctt
     4621 ggaatctcca tgaaaaagaa gaaggaacct atgatttttc gaaaatattg gatctttccc
     4681 attttctaga gttagcgcaa tcccttggat tatacgcaat cgtacgtcca gccccttata
     4741 tttgtgcaga atgggaattt ggtggcttgc ctgcttggct attgacaaag gagtgccgta
     4801 ttcgttcagc ggatcctcgc ttcatgaagt atgtcaaacg gtattaccaa acgctactac
     4861 ctaaaatcgt tcctcatcta gtcactcatg gtggcaacgt cttaatggtg caggtagaaa
     4921 atgagtacgg ttcgtatggt gaagagaaag catacctgcg ccaagtaaaa aaatacttgg
     4981 aggaaggtgg aatcgatgta ccgctattca cttctgatgg accttggcaa gccacattaa
     5041 gagcaggcat tttgattgac gatgatgttt ttacgacagg gaattttggc tcaaaagcaa
     5101 aagaaaattt tgcagatatg gcccagtttt ttgaagcaca tggtaaaaaa tggccgttga
     5161 tgtgtatgga attttgggat ggctggttta atcgttggaa agagccgatc attaaaagag
     5221 atccggaaga attagcggat gcagtcaaag aagcattaca gatcggcagt atcaatctct
     5281 atatgttcca tggcggaacc aattttgggt ttatgaatgg ctgttcagcc agaggcgcga
     5341 ttgatttgcc gcaagtcact tcctatgatt acgatgcacc attggatgag cagggaaatc
     5401 caacggaaaa atattttgct ctgaaacgaa tgatggcaga aaattttcca gaaatgcagc
     5461 aaatggaacc atggataaaa gaaacaatcg tcaaaaaagg aatcaacttg aaagaaaaag
     5521 tcagcttttt cgcaacactg gataccatca gcaagccgat ccactcgacc tatcctcaaa
     5581 caatggaaga attgggtcag aatactgggt acctattgta tcgaacaacg atcaaaaaag
     5641 atgcggtcaa tgaaaaatta cggatcatcg acggacgtga tcgggtccat gtttttctcg
     5701 atgaagaaaa gcaagcgatt caataccaaa cagagatcgg tgagaccatt ccattgacac
     5761 ttgagaaaga agaccatcaa attgatctat tatttgaaaa tatgggacgg gtcaactatg
     5821 gacacaagct gctagctgat acgcaaagaa aaggaatacg tacaggagtg atgagcgatc
     5881 ttcattttat cacagaatgg acacagtatt gtttaccttt agagactaca gaacacatcg
     5941 atttttcaaa aaaatgggaa gcaggtcaac cagcatttta ccgctttgaa gcggaactgt
     6001 cagaaatcgg tgatacttat ttggacctta gtgagtttgg gaaagggatc gtttgggtga
     6061 atggcacgaa tattggtcgt ttttgggaag taggaccaat tctttcactg tttgttcccg
     6121 agggtttatt tagaaaagga caaaatgaaa tcgttatctt tgaaacagaa ggtagattca
     6181 gtgaagttat agattttgtc aaagaaccaa tagaaaaaag ttagaaaagg agaatggatg
     6241 atggcaatta ttggagtaag aattgatgga cgtttgatac atggacaagt ggcaaatctt
     6301 tggacgacaa agttgaatat tggtcggatc atggtcgtag acgatgaggt agcaacaaat
     6361 gcgatcgaaa aaagtgggtt aaagttggca acgccagctg gagtcaaatt aagtatttta
     6421 cctattgaaa aagcagccgc aaatatttta gcaggtaaat atgattctca acgagtgctg
     6481 atcgtagcga aaaaaccaga tcgtatctta gcgctagtag aaaaaggtgt accgatcaaa
     6541 gaattaaatg tcggcaatat gtcacaaaca gatgaaacac gttccatcac aaaatcaatc
     6601 aatgtcgtag atgcggatgt agaggtcttc aagaaattag atcagcttgg cgttcatttg
     6661 atcgctcaaa tggtgccaaa tgacaaagca gaagacttta tgagtctttt aatcaaatag
     6721 gaggaatgat tcatggatat tcaatggtgg cagatattat tattaacatt atatgcaggg
     6781 tatcaaattt tagatgaatt acagatttat tcttcaatga gcgccccagt ttttgcagga
     6841 ttatatgctg ggttagtaat gggtgattta aaagcaggtt taatcattgg tggaagtatg
     6901 cagttgactg ttttaggggt agggacattt ggtggtgcat caaaaatcga tgccaactct
     6961 ggaacgatct tagcaacagc tttctccatt tctctaggga tgaacccaga acaagcgatt
     7021 gcggccatcg cggttcctgt agcaagtttg atgatccaat tagatatttt ggcacgtttt
     7081 gcgaatacgt attttgcaca tcggatcgat caacatgtgg aaacaatgaa ttacaaagca
     7141 attgaacgga acttcttgat gggggcatta ccttgggcac tttccagaat ggttcctgta
     7201 ttcttggccc tagcattcgg tggtggctta gtccaaaatg ttgtggctgt tttaaacggc
     7261 gacctaaaat ggctaggaga tggcttatct gttgcagggg cagtcttacc agcagttggg
     7321 tttgcaattt tactacgcta cctaccagta aagaaacaca tttcttatct gattttagga
     7381 tttacgatca cagcgttatt agcaacagtg ttctcgaatg tccaattgtt aggaacaagt
     7441 gtggctagcg tggtcaaaga tttcccagga acattcaatt cattaccgat gcttgctgtg
     7501 gcattgattg gtctagcact ggcaacgatc agttataaga atgatcaaaa gaatgaccaa
     7561 catccagtag taagtcagca atcacaatct gatgaggggg aaatcgaaga tgacgaaatc
     7621 taattataaa ttgaccaaag aagattttaa gcagatcaat cgtagaagtt tgtttacttt
     7681 ccaattaggt tggaactatg aaagaatgca aggttcaggt tatctgtata ctatcttgcc
     7741 acaacttaga aaaatctatg gtgatgatac accagaatta aaagaaatga tgaaaacaca
     7801 tacgcaattt tttaatacgt ctaatttctt taacacgatc attactggga tcgatcttgc
     7861 tatcgaagaa aaagaaggag tctcttcaaa ggaaactgtt tctgggatga aagcaggatt
     7921 gatgggttcc ttcgctgcaa ttggtgattc tgtatttgcg gcattgatcc cgaccatatt
     7981 tggagcttta gccgcaaata tggcaacaga tggtaatcca gtaggtatcc ttatctggat
     8041 ccttgctcag gtcgctgtca tggtctttcg ttggaaacaa ctagaatttg cctacaaaga
     8101 agggatttct ctagttacca cgatgcaatc acgcttgact gcattgacag atgcggctac
     8161 tttactagga gttttcatgg ttggagcgtt agtagcaacc atgatcaatg ttaaattctc
     8221 atgggcacca acgattgggg atgtaacgtt gaatatgcaa aactatatcg atatgatctt
     8281 acctaaactt ttaccagctg gaatcgttgg tggcgtttat tggatgttag gtaagaaaaa
     8341 tatgacacct actcgagcaa tattcatcgt attgatcgta tgtgtagcat tatctgcatt
     8401 aggtgtgatt tcgaaataag ggagaattgg aaagatgagt agacaactag tcttgattag
     8461 tcatggtaaa ttttgtgaag aactaaaaaa cagtacagaa atgatcatgg gaccacaaga
     8521 aaatatctat acgatccctt tattgcctga agatggcgcg gacacgtata aagaaagatt
     8581 cctctccgtt acagcaccat tgaaagatta cgtagtgctt tgcgatctgt tgggtgggac
     8641 acctgcaaat accgtttcca agctaatcat ggaaggtgaa acgatcgaac tatatgctgg
     8701 catgaatttg ccgatggtca tcgaattcat caattcgcaa atgattggag cagaaccaga
     8761 ttttattggt tcgggtcgtg agagtatcgt ttcagtgaac caagtgattg ctggcatggg
     8821 agacgatgaa gaagaataat caagcacaac ttcttgactg gtgatacaac tggatataaa
     8881 tacggatgta taaagaggcc gtgtataaaa aagccaacgt accgcgaaat atagttttct
     8941 atatttcaca gtacgttggc ttttatcgtc ataaaatcag aaatgatctg ggtcgatcga
     9001 agaagctagt tacaaataac tatttacgct tttgctgttt accagcattc cgaccattct
     9061 tcgcattgtt ttttggtgct tgtacttgtt tttttgtttg cttcgcaggt tcagcaggtg
     9121 tgacatcttt acgaggcgtc accacttgtt ttggcggatt ttgttttaat tcttccgcaa
     9181 cttgcgcttt taatcttggt ttcatgatga cattcgtaat gaatgtttgt agacaactaa
     9241 agatcccacc gactacccag taaagagtga caccagctgg ggcactgatc gacatgaaga
     9301 cgatcatagc gggtgatacg atcaacatcg tacgcatcgt tttcttttgt tcttcaggga
     9361 taccgatagt tgagatatag ccttgaagta aataagcaat accggcaatc gctactaaaa
     9421 ctaagctagg gttacctagg tcgatcccgt aaaaggttga ttcacggatg ccttctgtat
     9481 agcgagcagt gaaatatagc gcagaaaaga tcggcatttg gatcagaagc ggtaaacaac
     9541 cgataccacc agtcatactt aagccatttt cacgataaac ttgttgcatt tcagcttgtg
     9601 cagccatttg ttcttctcga gttgtcgcag ctttcaattt ttgttgcgta gcttcaactt
     9661 gtggtttcaa tgcttgcatc ttttcagatt ggatcattgt ttttttcgat tgactaatac
     9721 ctaatggcaa aatgatgata cgaacgatga ctgtcatcaa gataacagcc cagccgtagg
     9781 accaattaaa gttttccact aaatatgtaa tcgcttgtcc cattgggtga accaatatac
     9841 ggtaaatgat tcctgaagtg tcagggttac catttgaatc catgcggaca caaccggaca
     9901 agaaaagcat gatcgtgaca agcccagagc ccaataacca atttttccat ttattcataa
     9961 aatacttcct cttttataat ttttacacaa gcttaactat actaaaaaag cgctcataat
    10021 tcaatcaact tcatagaaat ttcatagaaa taaccgattg gaggatcaaa aaagacgctt
    10081 aatgttaaaa agaagaatcc agctaaaaat acttcagtat aaagcatact tagcacctcc
    10141 gttctctaaa ataagatgag caacggatat ttccttgaaa tcataatttt agcgcaaaaa
    10201 aaagaagtgg aatgtcaatt ttttccactt cttttttttt accattgagc cgatccataa
    10261 aaaagctgac tgccatggta taaggggcta actatccctg tgtcacttgt tctaactctt
    10321 tcattttctg gacaaccagt gctggtgtga tcgtttcttt catgaagtgg atcatttcat
    10381 caggtaaaca tgcacgttca gctacggctt gatagtcact ttctgttaaa tgtaaaccca
    10441 tatcagctaa actcgttggt aagttcaagt gatgataaaa cggcagtagg tgttcgattt
    10501 ctgctaattt attttcgatc atcagctgga caaggatacc gtatgcaact ttgtttccat
    10561 gaagttgctg atggctatca gggagtacgg tcatcgcatc gtgaatggaa tgagctccag
    10621 ctgttcggcc gtagtcatcg ccaaacccac caaccatacc agccaataaa atgtttgttt
    10681 ccacgacatc gataaaagct tgattgattt tttgctggtc catcgcttcc attgcctctg
    10741 cacttttctc taaaaggatt tcgcggcatc gtaacgctgc aaattccgct acttggactt
    10801 cgataggctt gatagttaac tgagagatga ttgcgtgaga ttcataccat tttgctaagg
    10861 tgtcaccaat accggcaatc atcatctcgc gaggggagtg caaaatgatc tttggatcga
    10921 tcaacactaa atcatttgct ttagggaaca tatcatagcg aagcatcact ccttcttcat
    10981 tatagaccac actaagtgga gtataagctg cgcacgtcgc agctaaggta ggtaagatga
    11041 tgattggtaa gtttgtctta taggcaatca attttcctag atctgctact ttcccgccgc
    11101 cgactgcaat aataccattg aaatccttct ttttgcataa agaaacaaaa ctgtcagcaa
    11161 gtggatcggt acattctcca ccataatatt caaaaatttt tgttattgat aaatctggaa
    11221 aaacatcttt tactgctttc caagatgttt ttccatggag aatcaaaacg ttgtcaatat
    11281 gccttttgta aaggtgttca gggagtgaat cccatgcgtt ttcttttatt tcatactctt
    11341 gaggggcacc gcgaacaatt aaattttttg tcatataaaa aacttcttcc attatttttt
    11401 taaattaatc tttttttatt ataatagtta tgctagcata ttagcaagtt atttatgaat
    11461 atttattctt ttatagcttt tctgattaca ttattattca aaggtggcca aatggatgaa
    11521 aaaaggaata aacatattat tagtggcgct gatgtttcta ctggtttttt taccagtaaa
    11581 agtacaaggt gcagaaaaag atgaaattta cgagcggata aaacaaagtg gagaattagt
    11641 cgttggccta tctgcagact atgcgcccta tgaattccat gcgaatgtgg atggtaagga
    11701 taaaatcgtt ggttttgatg tatccattgc cgaaaagatc gcaaaggata tgggtgtgaa
    11761 actaaaagta gaagaacttg gttttgatgc attattaggt gcgttgaaaa ctggcaagat
    11821 cgatttagtt atatctggta tggcggtgac tgaagaacgt ttacaagaag ttgacttttc
    11881 tgacccatat ttcgtggtac agcaaaaagt actcgttcgt aaagaggaca aaaataaatt
    11941 taagacgaca gccgactttt ctggcttagc cgttggggcg caaaagcaaa ccacacaaga
    12001 ggagttagtg aaaaatgaac tgactggtgc agagttgacc tccctgcaaa aagttcctga
    12061 tctgatcaat aatttgctga atgaaaaagt tgaaggtgtt gtgctggaac aaccagttgc
    12121 agaagcatat gcccaacaga ataagaaagt agctttgact ccagtaaaat ttgcgcaagg
    12181 cgaaaaaacc acagctattg cgatgtcaaa aaatacacca gaactgaaaa atcatgtcaa
    12241 tgcgtcaatc aaaacgatca atgacgacgg cttgctgaaa gattatcaaa aacaagcgaa
    12301 tcaattgatg ttccaagatg atcagtcctt tcttcaaaaa tacggtccgt attatttgat
    12361 tggaacaaaa tacactattt tattagcttt tgtgggtgta ttgtttggaa tggtctttgg
    12421 tagtttgctt gcactgatga aattgggtaa gaataaattt ttaaaggcat tagccattat
    12481 ttatatcgaa tacgtacggg gaacacctct cttagttcaa atctttatcg tttacttcgg
    12541 aacaggagta ctgggattgg atctatcgaa actggtagca ggttgtattg ctgtctcgtt
    12601 gaatagtgct gcttatgtag ccgaaattgt ccgtgccggg atcaatgcgg tcaacaaagg
    12661 acagatggaa gctgcacgtt ctttaggaat ggaccagaaa gaagccatgc gtttcatcat
    12721 cttcccacaa gcaatcaaaa atattcttcc agctctagga aatgagtttg tcacgatcat
    12781 caaagagtcc tcagttgttt cggtcattgg ggtatcggaa ttgatctttc aaacgggcgt
    12841 ggtacaaggt gctagcttca aaccattcct accatatgtg ttggtctctt tgatttattt
    12901 cgtgttgacc ttcagtattt cccgtatatt agctttagtt gaaaggagaa tgggtctgaa
    12961 tgcttaatgt aaaaaaactg tcaaaaagtt ttggcgagaa tgaagtctta aaatcaattg
    13021 atctttctgt gaaagaagga gaagtagtgg tgatcattgg cccttcagga agtgggaaaa
    13081 gtacaattct acgttgtatg aatctgctgg aagaaccaac ttctggtgaa atctttttta
    13141 agggacaaaa cattacagcc cctgacagcg atatcaatca aattcgtcaa aaaatgggaa
    13201 tggtatttca aagttttaat ttgtttcccc atatgaatgt actcgaaaat ttgaccatca
    13261 cacctgtaaa aatcaaaaaa gaagacccta aaaaagcaaa agaacatgct ctatcattat
    13321 tgaaccaagt tggtctaaag gaaaaagtgg acagttatcc taccagttta tctgggggac
    13381 agcaacaacg ggtagcaatt gctcgagcat tagcaatgaa tcctgatgtg atgcttttcg
    13441 atgaacctac atctgcactt gatcctgaaa tggtcggtga agtgttggca gtcatgcagg
    13501 atttagctaa aaaagggatg acgatggtcg tggtcactca tgaaatgggt tttgctaaag
    13561 aagttgccga tcgtgtgatt tttatggctg acggcatcat tcaagaagag ggaacaccga
    13621 aagagatttt tgaaaatccg caaaatagtc gaacacagga tttcttgaac aaagtgttgt
    13681 aacaaagatg gaggaagtaa gatggacaaa agtaagattg cacgccggtt tcagcaacca
    13741 gcagaaaatc tgttgatgaa tattgctatt ttggcaaagg aaaaagagga tattatcgat
    13801 ttatcgattg gtgatcctga cttgatcact gatccgacga ttattgaagc agcttataca
    13861 gatgtaaaaa atggtgctac taattatact gcgcctgatg gcagcaaaga attcttgtca
    13921 acggtcgtag atttttatca aaagaaatat ggattgagcg ttgaatgcaa ccaagttcga
    13981 gctacagtcg gagcaatgca cgggatgtat ctagttttac aggcattact agatccagag
    14041 gatgaggtca tcatccatga accttatttt tcaccatatc gggaacaagt cattttatct
    14101 ggaggcaagc ctgtttttgt tcctacctat gaagaaaagg gcttccagtt ggatgtggaa
    14161 gatttaaaag cagcgattac ggatcgtaca aaggtgatcg tcatcaattc gccgaataat
    14221 cctacgggag ctgttttttc tgaaagcacg ttgaaaaaaa tcgctgaagt cgcaatcgaa
    14281 aatgaactct atatcatttc cgatgagatt tatgaagact ttgcctttaa agagaagttc
    14341 gtaccgatgg cgcgatttgc gcccgataat acgatcactg tcagtggtgt atcgaagggg
    14401 tttgcgatga ctggctggcg cttagggtac atgatcgcac cagcatacat caatcaaatt
    14461 gctgggttga tcaatgaaaa catcacttat tccgcaccga ctgcttctca aagaggtgca
    14521 atctacgcct taaagcacta tgaacagctt gtaccgccag ttgttgaagt attcaaagaa
    14581 cgattagctt atattgagaa aagagtggca gaaatccctt acttatcttt atcttctgtt
    14641 tctggaagca tgtatgcttt tattaatatc agtaagagtg ggctcgattc agtaacgttt
    14701 gtggaagagg ttttagaaaa aagcggcgta ctttttgtcc ctggaaaagc gtttggggaa
    14761 acgatcgggg acaactatat tcgactagca gcgactcagc cattggaaat cttaggtgaa
    14821 gcctttgatc gtttagcaaa actaaatttt tagataaaac tgtatatttg aaaacagcta
    14881 tgggtatcca ttagaacaaa gagggaaaat gatcctttcg agagttggct tgttgactgg
    14941 gcagcgtcag gatttgacaa tgaaaaagtg gtagtggaaa atcaaagtcg attttccact
    15001 accataaaat attatagact ccattttatt gaaccaaaat ttttgaagtc ctcccgcttt
    15061 tacgtatgcc tatcactggt tactaaactc gctaataaga atgttttttt gtcgatctag
    15121 gatggagaga ggggcaaact ttttcgatct acatacagac agtccgattt ttctgacatt
    15181 tcctattcat ttctagaagg tcagcctatt acaacaaact tttttctgtc ctccatcatc
    15241 ggattttcct gaaggtctac atattggaca cgtccgctgg ggcttggtga tcgtttgact
    15301 tcttccacga attttttgat tgcctcatcg tcgcccgcag cttcgatcgt gacagagccg
    15361 tcatcttcat ttttcactgt tccggtgatc cctaattgat ccgctacgat ttttgtcata
    15421 tagcgaaaac caacgctttg tacacgtcct tgcacgttca tcttgatttt gttcatttta
    15481 ttcaccgttc tttcttttat ttttaaatta atgataccga cttttttgct actgaacaag
    15541 tgcaatgatc taatccatgg tataatcagt tgaggtgaaa aatgtgaaag agatccaatc
    15601 agcaaaaaat ccaagaatca aagaattaaa aaaattacat aagaaaaaat atcgtgaaga
    15661 aaaaggggaa tatctgcttg aagggtttca cctgatcgag gaggctgttg cctcaaatgc
    15721 gaaggtcaac gaacttttta ttacgcaacg cggtctgact gagtgggcta gttggcttga
    15781 agaaaatcag gcgctttctg ttacactagt atcagaagaa gtgatggcaa ctgtttcaga
    15841 tctaccaact ccgcaaggga tcatggcagt ggtggaagct gaaaaaagag agataccgaa
    15901 ttttgaaggt ggttggttgt tgctcgacaa cgtacaagat cccggcaatg tagggacaat
    15961 gatccgcaca gccgatgctt ttggtctatc ttgtgttgta ctaggtaagg gaacggcgga
    16021 tgtctacagt accaaagtat taagaagcat gcaggggagt aactatcatt taccgatcat
    16081 ccaagaggat ctaacaaaac tattgcctat ccttaaagag caaggattta taacctatgg
    16141 aacagaactg aatgaagaag cagtgtccct gaatgatatc cagacaacag aaaattatgc
    16201 gatcgtgatg gggaatgaag gtcaaggcgt acgtccagaa gtgctggcgc taacggataa
    16261 aaatgtgtat ataccgattc aaggaaatgc ggagtcttta aatgtaggag ttgcagcggg
    16321 catcatgctt tatcacttct ccgtatagtt aaggaactat taagctttct gcattttgcg
    16381 aaaacctcac tagaatatta cgaacactag tggtatacta agttcaattt caaagataaa
    16441 tggaattcaa aaaggtggca gatcgataat tatgacagaa aaatggcgtg aagacgaaga
    16501 atatatgtct tatataaatg atctattaga aactgatgca gtaaagaaat tagcaaatta
    16561 cacgcaacat gtccactcga cacgattaga gcattcaatc agtgtgtctt actatagcta
    16621 caaattagcg aaaaaatggg gcggtgatgc aagagcgact gctcgagcag gccttttaca
    16681 tgatttattt tactacgatt ggcgtacgac gaagttcgat gaaggtacac atgcgtatgt
    16741 tcacccaagg atcgctgtga aaaatgctga aaaaatcacg gagctttcag atctagagcg
    16801 tgatattatt ttgaaacata tgtggggagc aacgattgcc cctcctaaat ataaagaagg
    16861 ttatatcgtt acttttgtgg ataaatattg tgcagtcaaa gaagcagctc aaccaatgac
    16921 agcttcaatg aaacaaaaat ggcaacgata tttcggtaaa gaacaatcga tttaaggtag
    16981 ttaactaagt atttgacccg agtattttga aaaaaatcct cgggtttttt gtttagacat
    17041 tgactaattg gtctttttag tgtttacttt gttgagatga aaaagtacat aaccaacata
    17101 aaaataaagg gggtgatgat aagatgcgat gtgtatctta tttaaaagag tttgagggct
    17161 ttatctaaag gcactttgat agaaagatca cttttttctt caaagtagcc gattttgaaa
    17221 gaagaaagga ctcaaagtca tggaaaaaac aatcaaacca tatcaatatt attttatcaa
    17281 gcgagaagtc gagcagctgt taagcgccta tagttcagtg aatgatccaa agacagtcca
    17341 aacagtccaa gcattagctg cggaaaagat ccacgaggta cttggtcatc ctaatacagc
    17401 tgtaaatgaa tttatggaaa aggtactaga cgtcactcta aaaaaagaaa aagcagaacg
    17461 actattagag gaattgaaag tgatcgttct tccattcata caaccgacaa aaccgcagat
    17521 cgataaatta ttccgaaaag tgaaaaagtt aaaacaacca gattggaatc aactggattt
    17581 aagggaacat acttatattg gttggaatga tccaggtagc caaaaaaaat ttatgatcta
    17641 ttatgaagaa gaaaaactaa aagggatcag tggaacatta tctcctacaa tcaacaaagg
    17701 aatttgttcg atttgtcaaa gaacttcaaa tgtttcactc tttctttcta ccacaaaaac
    17761 tggttcagat ggaacataca cgaaaaaagg aaattatatt tgtcatgata gtgaccaatg
    17821 taatcagcag ttggtacaat tagctccttt ttacgaattc acagaacgag tgaaacgtga
    17881 gcggtgattt cattgtattt ttctgaaaaa tatgctatct tatttggtga agtgaatagg
    17941 tgatggtgtt cgcctttaaa cgttgatcgt aagataacta atgacgccta ctaaagaaac
    18001 ggggctaccc ctttttttag taggcgtttt tttgaggagg aaatgagatg gaaaatcgat
    18061 tacctgatcc attgaaggtg atcatctata tgatcaaatg gttattgatc acagcaattt
    18121 taggtatttt tatgggaagt ttatccgcat tctttctaac tagtttgact gatgtgacag
    18181 ctattcggct ggagcatcct tggttacttt ttcttttgcc gataagtggg ggactttttg
    18241 cttatctata tacgacgtat gggggcaatt cttcccgggg aaacaattta gtgatcgatc
    18301 aagggaatgg aggaaatgaa aagattccat tacggctgat cccactgacc ttatttggca
    18361 ccattaccac gcatcttttt ggtggttctg ttggtcggga gggtacagcg gtccaaatgg
    18421 gcggggcact tgctgagaat tttgctcgtt ggctaaaatt gaacaaaatc gagcgtgaga
    18481 ttcttgtcat cagtgggatc agcgcaggtt ttagctcagt atttgggaca cccttggctg
    18541 ggacattgtt tggattagaa gtattggcaa tcggaaaagt gagaaccgaa gcactgtttc
    18601 caagcttttt tgcagcactt tttgcgaact ttgtaacgga aagtttcaat gtcaagcata
    18661 cccattatca aatgggggcg attcctgcat gggacccttc acttttcttc aaattgttgc
    18721 ttgcgggcat tgcatttggt atcattggct ggctctttag ccgttcgatc gtctggttaa
    18781 aagcttggta tgcaaagcgg atcccccatc cagttttacg caactttatt ggaggagcag
    18841 tcgtggtact cgtagtagtg attttacaga cgcaaagata tctcggatta agcttacctt
    18901 tattacaaga tgcatttaat ggaagtgctc atccttatga ttttcttggg aaacttggct
    18961 ttaccgttct atcacttggc gcgggatacc aaggaggcga agtgacccct ttgtttgaga
    19021 ttggggcaac tttaggtgcg acattagcac ccattcttca tgtatcagta ccttttttag
    19081 caggattagg gtttattggg gtgttctctg gagcaacgaa tacaccgatt gcttgctttg
    19141 ttatggggat cgaacttttt ggaggtcaag cagccagcta cttctttatg atctgtttga
    19201 ttagctttat gtgttcagga aatagtggaa tctattctgc gcaaaaagtt gaagtgaaaa
    19261 aaggaatctt gttcctaccg atccttggaa gaatctcacg acaaaaagag aaataaaaag
    19321 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    19381 taaatttttg tgaatttcgc ttatttccga aggagttgct tctgctccca ccgtttattc
    19441 ggagttagag tctgtggcaa aactgatttt tagttttgtc ccacgctctt ttttattgag
    19501 caaatttgag aataagagca gaaaatactg caaagccaat cattaagcca cagatgataa
    19561 gatggcgttt tttgtgtaag acaaggatgg caataaattc tcttaagata gcattcggta
    19621 agtagtatag tgcagttttt gcaccgattc cgtttgcatt gagtttcgct tgtcgcgcat
    19681 atagtccagc tctaaagata tggtaattat ttgaagtaaa aatcgcgcga taaggcccat
    19741 tcatttgctg atccattatt tcttttgaaa acagcatatt ttctagcgtc gttgtggaat
    19801 tcgtttctac taaaatatca cgctcgggaa tccctttttc taaagcatat tgtttcattg
    19861 caatggcttc agctactttt tcatcggttc cttttcctcc tgacatgagc aagactggcg
    19921 gattcaaagt tgctttactt tgtgcccaat aaaatgcgat cgccttattg atccttttag
    19981 caagtagggg agatacgcgt tcaccatcaa tcaagccagc gccgagaaca acgatataat
    20041 cttggttata ccttggacgg ttgaactgat acaaaattga aacagtcata aaattaaaga
    20101 aaacgatgag tagataagca aaaataaacg gaatgacagc aaatagagca tttgcccatt
    20161 gtgggagcca atggactaat gtatagtcca atatcaaaaa gatagtcaaa gcgactccta
    20221 acaagagagt caaaaggttg gccaaagaac gggattcttt tttcatgact acaacaccat
    20281 tccaaaataa aaaaatgatc aaaatgtaga taccgattgc caaaagaatt aatattggta
    20341 tcccgatcaa agccaataac ccaccgataa agatattttg cgtctctatt agtagccacc
    20401 ctagataacc accaaagaca atcaaaaacg tattaaaaag tacgccattg attaacctac
    20461 gtttttcctt aaaatagaaa aatgcaaaaa tactgaaaaa tagcaatgga atcaaaaaat
    20521 aaaatgtatg ataaaaaact tctaaaacga cgaagtaagt gaccatcatc ataaaagtcc
    20581 acgtcccaca ataaaagaat agagcgggat cgctttggcg cttgcgccaa tagaggatgc
    20641 ctgacattcc taaaatatag ataactaaaa atatcaggtc tctagtaaga ttcattgaat
    20701 gaactccttt cgatttgtaa attttttctt aaaaaagata gcatcattta gtagtaaata
    20761 tatcgaatat agaaaagtta gttactaaat agtcaatcag ttttttcacg acgtttctat
    20821 tataacgaat tatgttgaaa aaagctttta tcgaaaacat aaatcttcaa attaacagat
    20881 tttttagacc tcgatcatga tgaagacttt tgtagataca aataactgta acattgaaaa
    20941 tgaatgcgaa aacgatattt ttttttattg gaaaatatcg gtcaagcaat aaaccagttg
    21001 cataataaaa tgttaacggc aacaaaatct gtggaaggaa tgaaccggat gatttgatgg
    21061 aaaaaacaag atagacaaga agtatacata gtaaaacgtg gaacaagtag gccagaatga
    21121 aaaatcagtt tttatccact cgcttttcta tacattcact gtcacaaata taatcgaaag
    21181 taattctatt ttgagataaa gtattttata ggagatagta tatgaacaaa aaaagattta
    21241 aacgtattga aagaaaattt gcattaactt atgaccaagc taagtatttg aaaacactat
    21301 ttccaaaaca ggtagttcgg gataaatata gtgatcattt tatttcttcc ttgtattttg
    21361 atacaaacga tcatcacttt ttaaaagaaa tcactacaga aaagtcctat cgagaaaaat
    21421 ttagaatcag aaaatatgga gatcctcagg acatatcgcc tatctactta gagataaaaa
    21481 gaaagtttca aggaatgaca gataaacgta gaataaaatt agcgcatcat gagctcgatg
    21541 atatgttgga aaatatcgaa ttggcagaaa ccacattcag taatttagaa gaagagtata
    21601 tttttaatga aatacagtgg ttgttcagaa ggttggcatt ggagcccaaa gtatatatct
    21661 attatttaag agaagcattt gtaaatagag attcagaaga tttgcgaatc acttttgatc
    21721 aaaatatcca ttaccgtcta atgaacttaa cgacctggga acactgtaca gaagacaatc
    21781 tggtagcgcc agaaatcgat gtgctgatgg aagtaaaaac gacaggagaa atacctgaat
    21841 ggttacaggt aggattggat aggatgggta aaaaagagat aaaattttct aaatataaac
    21901 aagtctataa aagatatatc caagaggacc cgctctatgt tcaatagtgt cttaaaaaat
    21961 gagccttctc aactcacact agatgatttc tttttatgta tggtggtgtc gattttgcta
    22021 ggcattctga ttgcttacac ccatatgtat caaaacaact gtacaaaaaa cttcatgttt
    22081 gccttgattg ggttacctac tttagtacat accgtcattt tgttgatcaa cggtaactta
    22141 ggcacgggga tcgctgttct aggcgcattt aacttagtgc gatttcggtc tgctcaaggg
    22201 aattcaagag atattttcag tattttttgg gcaacgtcga ttggtttagc cacagggacg
    22261 ggatatattg tctatgcact attcgtgtct agtatagttg ctgctatcca agtcatcatc
    22321 ttcacgcttc caatcaaaaa taaacataca gtttttaata ctgaaaaaac gatcaagcta
    22381 aaagtttcta aatccttgtt tctttcgagt attattgaag aaaaaattgc agaatataca
    22441 gaaaactgca atttaaaaaa agtaaaaatc aataaagaaa cttgttcctt acactatata
    22501 gtaaaagtaa aaacgagtat gcttgaaaaa gaattggttc gaacgatcat ggatcatcca
    22561 gaagtcattt cgatcactgt aggtatggat aaatcgaata aattattgtg ataaccaaga
    22621 aagtacttaa caatgaagta agaccccagt tccaaagttt agcggggaca cttcaataaa
    22681 gtactttctt ttttgtcttc agcaactact ggtaaaactg agagcaagct ttcgtgagag
    22741 tacagcgata aagatcaacc tcccttttta tctcgagcaa tttccttgca aggctgtttc
    22801 gttgatctta aacgagttag caacagtcaa agatgcgatg gccataaaaa aattgcatta
    22861 agttggctgg tatctgatag ctgggaatct aaggaagact atccattctg tttcttaaat
    22921 tccgctagtt gatagatatc agccaattgt tccagatgat caacatcaaa gataaccgaa
    22981 agatgtttcc ttttattccc atttgcttga agagttttta tcttctcttt ggttccttca
    23041 ctcaatgaga gtgtgactaa tttctccctc gttttttctg ggaaatgaaa ggatactttg
    23101 aaaaatccgt cccaaataga aagccagaag atcggtttta ctttctgaga gtgggacaaa
    23161 agtaagaaac acttttgtct cattctttaa aaacgaataa acggtgggag cagaagcaac
    23221 tccttcggaa ataagccaaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    23281 ttatttctcg gagttaaaca cttctgtccc aacctcttgt attcaccttt tgatagccaa
    23341 gcttttccat cattataaaa ccgcaaattt caaaaaaaaa cgaacaatac aaaaagcgta
    23401 ttgctcgttt ttctcttcag tttctcacac ctaaacgact tttgtctcga cctctttttg
    23461 gtgactattc atgatgttaa aataaattac tgaaaaaatc tgtgacatgg cgccaaccaa
    23521 tgacaaaaat attagctttt tcaacagtag aagtggtaat gatgtctgtc gaaggctctt
    23581 ggtttgcttc caaatagcct aatttgtcat cagctaattg gatcgttgct gagccaactg
    23641 tagtgccttt gttgatagga gcggtcactt ggtcatccgt tactttcttt ttatcaattg
    23701 ttggagtaat cgttaatttt ccagtatcca ttccattttc gacccagact tttacagggc
    23761 tttttagcgc aacattgacg gaggtttctt ttccatcttt cactttgatg tcttttaggc
    23821 ttgggatgct tgcatttgct tttccaagtt ctttttctga ccagttgtca taacaataat
    23881 ccatcaatcg tgatgtttca ttgaatcggg cacttggatt ttcttcatgg ttatctgcat
    23941 ttaaaacgac tgtgatgatt cgtcggccat ctttgttgat cgttccgaca aagcatgcac
    24001 ccgcaaaatc agttgtgcca gtttttaatc catcgacacc ttcgtgatag tttacaaaac
    24061 caggtagcat ccagttccaa ttgaccattt caaccggtga ctgtgtgttt tcgccgaatg
    24121 tttgatttgt ggtactcgtg atttttaaga cttcaggata atctaagatg aggtgacgag
    24181 caacaatagc gacatctttc gcagacagtt gattttcatc tgtttcacca gtattctcag
    24241 gtcgattctc acctaggtaa gaattgttta aacctgatgc attgacgatc gttgcatctt
    24301 tgatacccca atcttttagt tgtttcttca ttagctcaag aaatttactt tcactgcctg
    24361 caactttttc agctaaagca accatggaag cattggctga ttggatgaaa gctgagtcaa
    24421 caagttcttt cactgtataa tcattttctt tatgcaaagg aacattcgat agatccggca
    24481 cgacacttaa tttttctgca taatccgaaa tggctacctt atcttcccaa gataatttac
    24541 cctctttgat ttgttcaagt acgatatata gaccaatgat ctttgtgatg gaagcaattc
    24601 ccatcgtttt ttcgccatcc tgatcataaa ggatttttcc actttctgca tctacggcaa
    24661 aggcagcttt ggcatttatt ttaaatgcgt cgtctgcttg aacagttgcg ctaggcaaga
    24721 aagaagtgat acataagata agacttgcta atagtacaat aaaacgggta ttttttttga
    24781 acatctttgt tctcctcatt tttaaattga ttcttacaac ttagaatatt atcagagaga
    24841 acaaaaggtt tcaatggttc tgaaaaaaac cttataaaga acaagtctta ttaatgaaga
    24901 atcatagaaa gttatctttt gggttcattg acacgaactt tgactggctg aacttcgggt
    24961 tcggttaggc gtgatgctgc aaaaagggcg cctgctgaga gtgcgaccaa actaaaaatc
    25021 atgaataatt gcataagatc acctcgttct tttttgtttt tttatagtat agcaagagag
    25081 tttgaagcta ctgttaagga aatgagcata taattgatga aagtatgaaa aaaatatgaa
    25141 gtaggacaac acaactaaaa atcgatcagg tatttctctg gtagggattc gttttattgt
    25201 attgataaaa tgggatgtgg tagactttat ttacagatac aaagaaatga tttttttagg
    25261 aggggtacgt ttatggcaaa aaaagaaaaa gaattattat cacaattgac ttcagaaggt
    25321 accaaaggac cgtttgtcac gattatgtta aacacccatg ttgcgcatca agacgtagaa
    25381 aaagatcaaa tcaaattcaa gaattttgca aaggaagcga aaaagcgctt tgaaaagaaa
    25441 tactctgagc atgattggtc tccattccaa gaaaaaatta ctagtttgtt ggacgatccg
    25501 tctttctggc gtagtgccac aacgagtgta gctgtcatag tatcaccaga acaagtgttt
    25561 atccaccgtt tgggtattcc tgtagatgac cagtattatg tgagtgatct accttattta
    25621 ttagcgatca tcaaaaatgg acatttcaac tattcatact atttattagg attgaatcgt
    25681 gattcgatga aattgtacga agtaacgaat aaacaagtca aagaagtggc tttgcctgaa
    25741 gatgcaccaa gagatgtcgt cactgcttta ggatctgaac taacaggagg cgggttgaac
    25801 tacgcatcaa atggtagtga aggtacgacg ttccatggag tcaatcctaa agatgaagaa
    25861 gtcgaaattg attgggtcaa ttattatcaa gcagtcgatg actatttgaa agatacattt
    25921 gaaaatccag agaaatggcc tatcttctta tttgcattac cagaaaatca gacgatgttc
    25981 aaaaaagtgg cgaagaaccc ttattatcgt tccgatgttg cgatctcagc ttcacctgca
    26041 caactttcat taaaagaaat tgaagcaggc gcaaaaaaac tatcgcaaga attagctgtc
    26101 aaagaagcaa gtgactacaa taagttgctt gataaaaagt tcattgatca actcgttgat
    26161 attataccgg cagcaaaaga agggaaaata tcccatttat tcattgctac atctaattta
    26221 gcagatggtt ttggcgaaga tccagaaaca gaatatgacc gccgtcaggt gctgaatgaa
    26281 ctagcagata atgtgatcac gaacggtggt caggtcttta tccttgaaca aaaagatgca
    26341 ccagacgaaa agagtttagt tgcgatctta cgttattgag tgaaaactac caaagtagat
    26401 gaatggtcag gcgttgatag aagtgtatag ctccgagaga aggagtttcc tctgtttcag
    26461 ccgttggatt catcttgagc gagagacaaa agctagcttt tgtctctcgc tcattatttt
    26521 tgtgctttag tcccattggc tttgcgaggg gaagatactt ttctcaaaaa aacgaacctt
    26581 gcagttgaga gggggataga tgcgcatact aatttttatc agttaaaaaa tgatgtggat
    26641 cattagagaa ttccaaagtt cgttcatcct ttttcagata ataaattgaa attttttgca
    26701 aaagtgaaaa tagcaaatag aaattttctg aatcttatag gggtgatttc gagaaaacgt
    26761 tcgtttattt gcaaatatat cttctaaata agagaataaa tacaaaaaaa taaggaaaaa
    26821 ctctggcaat tttgtaaacg gtatgatata gtaagaatgt agtttttgtt cggtaatctg
    26881 tggtagcttg tttcaaataa acatcttcca gatatacacc attcaagtaa ttgcaaaata
    26941 tgtgttgata cacaaggagg atttttatta tggaaacagg tacagtaaaa tggtttaact
    27001 cagaaaaagg atttggattc atcactgcag aaaacggaaa cgatgtattc gttcatttct
    27061 cagctatcca aggcgacgga ttcaaaactt tagaagaagg ccaagcagta actttcgacg
    27121 ttgaagaagg tcaacgcgga cctcaagcaa caaacgttac taaagcttaa tttacctata
    27181 ttaaaaataa aagtccagac atttatgtgt ctgggctttt ttgtcttttt ttccttaaaa
    27241 agcgatgaga gtatttttct gcactttgta gtataatttc aaaagaacat tagaggcagg
    27301 agaagaggaa gatattttga aaccaagaac ttatgcagtc gttgatatcg agacgactgg
    27361 gacgaatcca aaagaagatc ggatcatcca atttggctgc gtcatgattg aagctggtcg
    27421 gatcacttca cgtttttcga tcgatattca tccagggcgg aagatttcaa aacaaatcca
    27481 gcatttaaca gggatcacga atcaacgcgt ccaaaaagcg ccttattttg aagatgtcgc
    27541 ccaaacgatt tacaatcttt tggcagacac catatttgtc gcgcataata tttattttga
    27601 ttataatttt ttgaaccatg aactcatgcg atgcggactt cctagtttga agataccggg
    27661 catcgatacc gtagaacttg cacaaatttt cttgccgacg gaaccaagtt ttcgcttagc
    27721 tgatttgtca gaaagccttg gctttattca tgagaatcct catcaggcgg atagtgatgc
    27781 cgaagtgaca ggtcagcttc ttttactgat cgaagaacga atgaggaaat tgccgatcat
    27841 cacattagag cagattgcac gattaagtag ccatacagga atggatacga gtcgtttcat
    27901 ttaccacgtg cttgaagaaa tgaaagaaaa cccggagccc cttgatctct cattagaaat
    27961 catcgatggg ctagctttgc agaaaaaaga agtggagcta ttcacatccg tccattatgg
    28021 cgagcggaca tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta aaacactgat
    28081 gtatcgaaaa gaacagaaca ggctgatgaa tgctgtctac gaccatttca caaaagatga
    28141 atcaaaagat ttgatgatcg aagcagctac aggaatgggc aaaacgattg gttatctgtt
    28201 gcctttgagt tatttggcta cacctgaaaa accagctgtc atcagcaccg tttcgcttgt
    28261 tttgcagcag cagatcatgg aaaaagacat tccattactg aatcaattgc tagatcagcc
    28321 gatacaacct gtgattatca aaagctatcg ccactatatt gatttacagc gttttaaagg
    28381 tacacttgtg gaaccgccgg gacaaaaaca atatgctttg taccaaatgg ctgtcttagt
    28441 ttggctaaca caaacgaaga ccggtgattt ggatgaatta catttaacca atctgaacca
    28501 tgcattcttt gcagatgtcg cgcacagagg cactgggttt ttagcaagga atcagtcatt
    28561 ttacaaacaa gactttgtgc gtttcttgca aaagaaaatc aaacaaagca attttttgat
    28621 agtcaaccat gcatttttag tacaagaaac acagcgaaaa gaaccattgc ttccagctag
    28681 tccttatttg ttgattgatg aagtgcatca tttgccggaa attgctgaaa aagtaaatga
    28741 caaacgacta agtattaact acttccataa acaaattcag caactggaag agccgaatca
    28801 gttatttgac cgcatacaaa gttatttgcc gaaggaacat gaagccttgc gctggttgtc
    28861 gatttatcga gaagaactct ctgcactatg cgaagcgcag atttgcgtgg cagagggatt
    28921 aatgaactta gtaagcagca agcaacctaa cggacaatat ccagaagaag tcatgatgac
    28981 aaaagaaatg ttggatcagc tgccactgga atctggaaca gcaatggatc atctgcaact
    29041 tttttatcaa gagatccaac aactacaatc acagatgcgc ttgacttttg agattatcca
    29101 agaaagttgg accaaccaga tgcggatcga tttagcaagt ttatatagct tatttgatca
    29161 attagacaag caatacttgc tgatggacca atggctagaa gactggcgag atcatttgat
    29221 ccattggttc gttcccggga atacagcaca gcagatgatt tttcatctcc ttgattttca
    29281 agcagctatg ttgccgtcaa ctgtgtggta tcctcgatat gaccatattc tttatattgg
    29341 aggaacactg aagatcggac ctaatcgaca gtatctctct aaacgacttg gcattgaagc
    29401 agcacctctt aaagtgattg cttctcctta tgattatgag aaacaggcaa gaatctatgt
    29461 gccgaaagaa gcacctgcag tccatcaagt cagcaatcaa gcatatatcg attatttaga
    29521 agcagcactc acaggattga ttgaacaaga agatcggccg atccttgttt tattcacttc
    29581 tcatgatgtt ttgcaaaagg tatatcagaa aatgcatttg tctatgctag aaaaaggacg
    29641 tgaaattctt gctcaaggat ttggcgggag tcgggaaaaa cttttgaaac gcttcatttt
    29701 gtcagaaaaa agcgtcttat ttggtgctga cagcttttgg gaaggcgtag atttacctgg
    29761 agatgctttg cagattgttg tagtcacacg tttacccttc gacaacccgt tacgtccgat
    29821 ggtcaaagct aggaatgctt atctggaatc agaggggatc aatccttttt atcaggaatc
    29881 cgtgcctaaa gcagctttga agttacgcca agcattaggt cgtttgataa ggacggagac
    29941 agatcgtggc gtattactta ttttggatcg gagattagtc actgcaaaat atggtgcacg
    30001 aatcatccat gcattaccga aaaaagtacc gataaaagag ctagctatag atgaaatcta
    30061 tcaggatatc catgaattct taaagaaaaa tgaagaagga agttaaaggt gccgtctttt
    30121 ttcatcatta aaatctgcta taatgaaaag gaatgaagat aaaggagcga cgacccttga
    30181 agaaaaaaca gacaaaggaa aaaacgatta atcgcgtgtt gcttggcttg atcgtatttt
    30241 tattagcggt catcgtgatc agctcgattt tttacttgcg ttccagtcgt ccggcggtcc
    30301 aggcgaaaaa agaagcagta gaaatcgcaa agaaatatgc aaatctcgag gaagccgatc
    30361 atttttactg gtttactcga aagaaaacgt acttttcatt gacgggtacg aatgataaag
    30421 gaaaagaaat cgccgttatc attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa
    30481 caaagggact gactgaaaac gaagcaaaac agacaattgc tgcggcccat cctgaaatcc
    30541 aaatcgaaaa agctgcttta gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa
    30601 aagaaacaaa tgggaaactg aattattacc tgattggctt tgaatctgga gatgaagtca
    30661 agacaatcaa gggaatttaa aaggtaaagg aatgaatggg atggagttat caaaaagaac
    30721 aaaacgatta gaaccgtctg tcactttggc agctgcggca aaggccaagg cattaaaagc
    30781 aaaaggcaga gatgtgctga gtttgactgt gggagaaccg gattttgcga caccagaaaa
    30841 catccaagaa gcagcaatcg aagcaatccg caatgggaaa gctagctatt atacaccaac
    30901 agcaggaatc ccagagcttc gacaggcaat cgttgattat ctgaagaaaa actatggact
    30961 tgtctatgaa ccttcacaaa cagtcgtaac agacggagcg aaatttgctt tgtataccct
    31021 tttccaaacg atccttgacc cgcaagatga agtcatcatt cctgtgcctt actgggtcag
    31081 ttacggtgag caagtaaaac tggcagaagg acttcctgtc tttgtgaaag gggaagaatc
    31141 caatagctgg aaagttactg ttgagcagct agaacaagca cgttcagaaa aaacaaaagc
    31201 cgtaatcatc aattctccat cgaatccgac tggaatgatt tatagcaaga atgaactgca
    31261 ggcaatcggt gaatgggcag taaaaaatga tattttgata gttgctgatg atatttatgg
    31321 ccgtttagtt tataatggca atgagtttac tccaattgca acgatttcag aagcaatcaa
    31381 aaatcagacc atcatcatca atggcgtgtc taaaacatat gcgatgacag gctggcgcat
    31441 tggttatgcg gtggggaatt ctgagatcat caatggaatg attgctgttg cttcccagtc
    31501 aaccagtaat ccgacagcag tcagtcaata tgcagctgtt gaagcattga aaggcgagca
    31561 agatacagtg gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt tatatccgct
    31621 tgtggcagaa ctgccgggtg tttctcttga aaagccacaa ggagcttttt atctttttcc
    31681 taatgtaaaa gaaacgctac gcatgtgtaa atatgaaaat gtcacacaat gggtagaaga
    31741 tcttttagaa gaaacagggg tagcgcttgt gactggcgaa ggctttgggg caccagaaaa
    31801 tgtccgcatg agttatgcca ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa
    31861 gcaatttatt gagagtaaaa gtcaaaatta atcctgaaga aattcaagga atataaaagg
    31921 agagtcatac gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt
    31981 aacaatcggc gcttgggtcg caaataagcg atcaagcgga aaaatcgcat ttttgcaatt
    32041 aagagacgga acagcctttt tccaaggtgt agttgtaaaa agcgaagtgc cggaagaagt
    32101 tttccaaaca gctaaaaatc tacaccaaga aacatccgtg ttagtaactg gagaaatccg
    32161 tgaagacagt cgttcaaaat ttggttatga aatcggaatt acaaatgtgg aagtggtaag
    32221 cgagagccac gattatccga tcacaccaaa agaacacgga acagacttct tgatggatca
    32281 tcgtcattta tggctgcgtt cttcaagaca acatgcgatt atgcagatca gaaatgaaat
    32341 cattcgtgca acgtatgaat tctttaatca acatcatttt gtcaaaatcg atccgccgat
    32401 cttgactggt tctgcaccag aaggcactac ggatctattc gaaacaaatt actttgatca
    32461 aaaagcttat ttatctcaaa gtggccaatt atacatggaa gctgctgcta tggctttcgg
    32521 aaaagtcttt tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt
    32581 gatcgaattc tggatgatcg aaccagaaat ggctttcact catcaagaag aaagtttaga
    32641 agtacaggaa caatatgtag cattcttagt acaaaatgtt ttagatcatt gcgaatacgc
    32701 attagatgta ctaggacgag acaaagaagt cttaaaaact tatacaaaat tgccattccc
    32761 tcgtattact tatgatgatg cgatcgaatt actgaagaaa aatggatttg atgacatcga
    32821 atggggcgaa gactttggtt cacctcatga aacgttcatt gcgaattcat tcgaacagcc
    32881 tgtctttatc ctaaactatc caaaagccat caagccattc tacatgaaac cacatccaac
    32941 aagagaagac gtggtgatct gtgcagatat gatcgcacca gaaggttacg gtgaaattat
    33001 tggtggaagc gaacgggcaa cagactatga tttcttgcta gaacaaatcc gtaatcacgg
    33061 tttggatgaa aaagaatatt catggtatct tgatctacgt aaatacggtt ctgttccgca
    33121 ctctggtttt ggtctaggcc ttgaacgaac agtcacttgg attgctggta tcgaccatgt
    33181 gcgtgaagct attccattcc cacgtttgtt gaaccgaatt tatccataag aggttgtgaa
    33241 atcagtgcct tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta
    33301 ctgttttttg agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg
    33361 tgcgtctgcc gttcagctcc gagtcacaag gagtaatttc aaaaaaacat tttttcatgt
    33421 ttgatgacta ttgtgacttg tgaccaagga gttggccgga gagcaacgtt tatttaaaaa
    33481 aaagaactgc gaactctcgc agttcttttt ttttaaatac gggataaagg aagtttccag
    33541 cagataagag aagaaagcaa aagaaattgc tatttgattc acaaggttct ataacgaaat
    33601 cgcaatcaat caaacatata caaagaaggt tattcaaatg gaaatctgat ttgatgatgc
    33661 ttctgctgtt gaaataaagc agcatctagc acctgaaaac aaactaatag taacattcga
    33721 agatggagta ggtccgtatt ttcagcatgc aatgatccat atgtaagttc aattcaccct
    33781 tgaaatcatc ggaccagata aagttatggc caatctatcc caagcaatct agaagagata
    33841 ttagtaaagg aacatttact ggaagtctta gatgagccta taaatatatg ttctgatggc
    33901 gtcatcaagt ggtctgattg acggaagctt aggtgtgatc gatctctctg gaccaaacaa
    33961 tgaaggaatc aaggggaatc cagcacgtta agaaaaaaat agcataaaaa actagacact
    34021 tgatgagcgt tcattcaatt gtttgaaggg actaggctgg tgtcattttt tatacatata
    34081 ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat catcgtttta
    34141 ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg acagcattac
    34201 agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc cacttttgat
    34261 tgccggaaaa ctaatctccc aatatccaac gatggaattt gaatgcatga accgtgggat
    34321 aggaggagac aaaattgcag atctgcaagt acgctggcga gaagattgtt tagctttaca
    34381 acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata ctagcgatca
    34441 ggctatattc ggaactgaga aatctgctca gcaatttgaa tccgtttatc gaaaattact
    34501 aaatgaactt caaaaagcgg gaatctgcaa catcatcttg atggagccgt ttgtacttcc
    34561 tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc aaattgttcg
    34621 taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat tcaatgcttt
    34681 agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc caacgattgg
    34741 cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg atttttttag
    34801 tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa
    34861 gaagatctac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt cgtaaaagca
    34921 aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt aacaaaagaa
    34981 aaacggatta tcggttttgc gcaagcggac gtgcaacagt cagaagaacg ggtgggtcag
    35041 cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc
    35101 caaaaaaaat ttgcagaata tttgatgctg gaagctaaaa aaagaagaat aaaatcaatt
    35161 tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt tgtttaggaa
    35221 atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta gcagcgggga
    35281 tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt aagatcgcct
    35341 tcctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc atgtcattga
    35401 tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca catgtcgtca
    35461 gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa tctttcaaac
    35521 ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaagaga ataataaagg
    35581 ttagataaag catagagtca ggtgggatac atgtatttca cctgactcta tgcgttttcc
    35641 tgtataagtt cttccatgtg acttttacgt cttttattta tatggggtta ctgtttatgg
    35701 aaagttgaaa taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct
    35761 tcgtatatct gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa
    35821 ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag
    35881 atcaagaagg caagtgcggg ttggataaga gcagtggcaa tgatcgaaga gaaaaagaaa
    35941 gaataagcca aaacatctgt atggaagaaa ttttccgatg cagatgtttt tcattttttc
    36001 tttcggatac cttgacatat gaataaccat tcatttataa ttagttatga agagatattc
    36061 atatgttgag gcgaggaggt cattaaaatg aaaagttaca gattagaagg tttagattgt
    36121 gcgaactgtg caatgaaaat cgaaaaagga gttcagaaaa tcaatggagt caaggaagca
    36181 acggtaaact tcacttctgg aaaactaacg attgacgcgg aggaagatca tttggctacg
    36241 attgaacaag aaacaaaaaa agtagtgaag gaattagaac cagatgtcaa agtaacagaa
    36301 atagataagg aaaaagtatc tgaacatgga aatgaaaaag agagaaatac gctgttccgg
    36361 attttatttt cccttgcagg tatcgcttta ttgctattat ttgattttaa tgaactaatt
    36421 cgcttgatag ggtatttatt gatttatctg ctgataggat atgatgtagt gaagaaggca
    36481 gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc tgttgctacg
    36541 attggcgcta tgttgatcgg tgaatatcca gaagccgtag cagtgatgct attttatcaa
    36601 atcggcgagt atttccaagg attggcagtc agtcattcaa gaaaatcgat tcgcgaattg
    36661 atggcgatcc gtccagaaac agctcacgtg cagacggcgg aaggattgat gaccgtgaac
    36721 cctgaagacg tgctgatcgg tcagtttgta cttgtgaagc ctggtgaacg tgtaccgtta
    36781 gatggaacga tcatcgaagg agaatcatta gtcgatacgt cagcacttac aggagaatca
    36841 gtccctaaat ctgtatatgt tggtgaaaca gtattaagcg gatttatcaa taaaaataaa
    36901 ccatttttag ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa attattagaa
    36961 ttagtagaaa atgccagcag taaaaaagca ccagctgaaa atttcattac taaattcgcg
    37021 cgctattaca caccagtcgt tgtaggattg gccgttttat tagctgtttt acctccactt
    37081 gttgtttctg gagcggcgtt ttctgaatgg atctatcgag cattgacatt tttagtgatc
    37141 tcatgtccat gtgcattagt gatttccgtg ccacttagtt tctttggcgg tatcggagga
    37201 gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact tggagttgct tgcgcaaacc
    37261 gagacagtgg tattcgataa aaccggcaca ttaacgaaag gcgacttttc gatccagacg
    37321 atcgatacaa aaatcgatcc aaagatattc atgcaatata ttgcgagtgc agaacagttt
    37381 tctacacatc cgattgctca atctgtttta gaaggctacg caggtccgct tttaccaaca
    37441 gctaatatcc aggaatttgc aggtgaaggg atcttagctg aagttgatgg caaacaagta
    37501 ttagtaggta atcacaaatt gatggaaaga tttgaaatct cttttccttc aagtcaagaa
    37561 atcggtacat tgctttatct agcaatcgat caaagttaca gtggttatct tgttattgca
    37621 gataccttga aagaagatgc cgtagacgca ttggttcaat taaaacaggc tggtgtgaaa
    37681 aacacagtca tgttgacggg cgactcgaaa aaaattgctg atcatatcgg gaagcaagta
    37741 ggcgtggaca agatatatag cgaattgctt ccagaagata aagtgcagcg gttagaggaa
    37801 atcttacaaa gtaacaataa aaagaccgca tttgtcggag atggaatcaa cgatgcaccg
    37861 gtgctagcaa gagcagatgt aggaatcgca atgggtggat tgggaagcga tgctgcaatt
    37921 gaagcagcag atgtagtcat catgaatgat caaccatcga agattgcaga agcaatccat
    37981 ttagcaaaga aaacattgaa aatcgtcaaa caaaacattg tctttgctat tgggataaaa
    38041 attttggtac tttccttagg tgcttttggt tttgcatcaa tgggagatgc agtatttgca
    38101 gatgttgggg tgacagtatt agctgttttg aatgccatgc gaagcttgca tgtaaaaaat
    38161 aaacgttgat caatgaagag gacgaatgaa cgtcctcttt tttttatatc cttttcttaa
    38221 tgaaaaatgt aaagaaaata gataggatta taaaataact aatgttttta attttgcaga
    38281 aaattcaaaa tagcttgaaa ttcaataagg tcaatgaaat aaaagaaaat aacttatttt
    38341 tttgatggaa aaaatatgaa aaaacgatgt gtattaaata aaataataca cacataaaat
    38401 aacactgtct tgtaagtttg ttaaaaattg aatatgatta catctggata acaaattttt
    38461 atcataaagg agagaacgaa tgaacaacaa acaattacgc tggtttactg ttgcgttaat
    38521 tgcatttaac atggtttggg gcatggggaa tgtagttaat aactatgctc agcaaggagt
    38581 atcagttgtc acctcttggc tattgatttt ggccatttat tttattccat acgcattgat
    38641 cgttggacaa ttaggatcag ccttcaagga tagtaaagga ggcgtcagtt catgggtcga
    38701 aaatacgaca tctaataaac gactggcata ctatgccgct tggacttact gggtcgtcca
    38761 tattccttat ttggcacaaa agcctcaagc aattctgatt gctggtggtt gggcagtcca
    38821 aggaaatgga aatatcgtaa atacgatgtc agtacaaatg gtagcagtta tttcacttat
    38881 tatatttctt gcctttttat atttatcaac aaaaggactt tctactttga aagtgattgg
    38941 cggactggcc ggaacagcga tgttcgtcat gtcgatctta tttattcttt tagctgtaac
    39001 ggcaccgttc atcaatccaa cgatggaatt tgccacacca catatgaatc aagtgaaaac
    39061 atatattcca acatttgatt tcagttattt cacaaccgtt tcgatgctag tatttgcagt
    39121 tggaggggcg gaaaaaatat ccccttatgt caactctaca agaaatcctg caaaggaatt
    39181 tcctaaaggg atgatcttct tagcagcaat ggtagggatt tgtgctgtat taggctcagt
    39241 cgcaatgggt atgctgtttg ctagcgataa tatccctaaa gacttgatgg caaatggggc
    39301 atacagtgct tttcaaattt taggtaatta ctatggtatc ggtaactcat tgatgatcat
    39361 ttacgcattg acaaacatga ttggacaaat ctccgcatta gccttttcaa ttgatgcacc
    39421 actaaaaatc ttgttagcag atgcggatcc agaatttgta ccaaactggc tacgtaaacg
    39481 taatcgtaaa ggtacattgg tgaatggtta tcttctgaca ggtatactag taggaatcat
    39541 tattttgttg ccgatctttg gtatcaaaga tatgaatgaa ttagtaaaat ggttgacgaa
    39601 cttgaactca gttgtcatgc caatgcgtta cctatgggtg ttcttggcat atatgatgtt
    39661 gaataaagct tataagaaat tcaattctga atataaattt gttaaaaatc caaaaatcgg
    39721 ttttatcttt ggtgcttggt gctttgcctt cactgcgttt gcatgtatcc taggaatggt
    39781 gccaaaagta gattatactg ctgatcctaa agcatggtgg ttccaattat tcacgaatat
    39841 catcacacca attgtcttga tcttattagg tatgatcttg ccagcaattg ctcgtcgaga
    39901 caaaaacaaa gaaattactg aaccagaaac agtggttcag ccttaaaata gaaatacttg
    39961 ttacaagagg ttgtgacaga agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa
    40021 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcat attttgccgt
    40081 tttttgagct aatgctgaag gtcagctgat tgaacaccat ttattcgaaa caagggactt
    40141 tgacacgact tttgtcacag tcccttgttt ttttgcggcg ttataagtgg tatgatcgaa
    40201 ccaaattcca aagagaagtg agggagaaga atgaagatca tcgtgattgg acaaagtggg
    40261 tcgggaaaat cgtccctcgc aaggaagatc aaagaaatca ccggctttcc gcattcaggc
    40321 aaaaaagtgg tttttgcaag aacagcaatc gtttatagta tctaatccgt catggatcgt
    40381 tgaaggaaat tatacttcta cgttagagga aaggattaaa gaagcagata agatcatttg
    40441 gcttaaagtg ccgcgttatc tcgccatcta ccgagtagtt tggcgttctc taaaaagaaa
    40501 gataaacaaa aaatctcggc cagatatgcc tgaatcattt tcggagaaac taaacaggaa
    40561 atacttagaa tttttatctt ttatctggca ttttgaagga aacaatgaac caaaaataca
    40621 gcagctgatc caagtagcta atgcaagaaa taaactgatc atccttaaaa caagacgaga
    40681 gaaacaagaa tttttaacta aattgaaaat agagagcaaa aataattgac agtttttgtt
    40741 tacaagcgta atctgtattc ataagctaat gattacattt gtaaacgaaa ggtgtgaaat
    40801 aaatggaaaa agtcaaaacg ccagtaaaga tcagtgattc agagtgggag atcatgcgtg
    40861 ttatttggac acttggacag acgacagccc aagaaatcac gcagatctta gctgattcga
    40921 tggattggaa accagcaacg atcaaaactt tgctgggacg tttggtaaaa aaagaagtga
    40981 tctggacaga acaaaaagga aaaaaattca tttatcatcc agctgtctcg gagatggaaa
    41041 cagttcgcag tgcgacggaa aatctttttt cccatatctg tgcaaaaagg attggagaga
    41101 cgattgcgga tctagtagaa gaagctactt tgactcaaga agatatcaat gagattttga
    41161 aacaactgga acaaaagcaa ccagtagata cgatcgaatg taactgtatc ccaggtcaat
    41221 gtgaatgtaa acaacatcaa taggaggaag aaaaaatgaa acaacaattt tcaatcgaag
    41281 gtatgagctg caatcattgt gcagcaagag tagaagaaac agtaagcgca cttgacggcg
    41341 tgcaaaaagt gaaagtaaat ttgaaaaaag caaacggtac ggtcaagttt gatgaaacaa
    41401 aagttcaatc cgaaaagatt tgtcaagcaa tcaatggatt gggttataaa gcagaggtga
    41461 tttgatggca acagcagaaa ccaaaatgga tacgttcgtc attacaggta tgacttgtgc
    41521 aaattgttct gctaggatcg aaaaagagct gaacggacaa cctggtgtgg tcaatgccac
    41581 agtcaacttg gccactgaaa aagcaagtgt caaatatgaa gggacgacca ctgaaaaatt
    41641 aatccaaagc gtagaaaaca tcggttatgg tgcaattttg tacgatgaag cccacaaaca
    41701 aaaaattgca gaagaaaagc aagcgtattt gaaaaaaatg ctattcgatc ttatattgag
    41761 tacggttttg actttgccgt tgatgttgtc aatgattgct atgatgttag gaagccatgc
    41821 ggcgattgtt cacttttttc attttccaat cgtccagtta gtgctgtctg ctcctgtcca
    41881 attttatgtc ggtgctcgtt tttataaagg tgcgtaccat gcaatcaaga cgaaagcacc
    41941 aaatatggat gtattggtag caattggaac atcggctgcc tttgcattga gcatttacaa
    42001 cggatttttc cgtggacacc cccaagatct ttattttgaa agcagcagta tgatcatcac
    42061 gctgattttg ctcggcaaat atttggaaca tacagcaaaa acgaaaacag gtaatgcaat
    42121 caaacaattg atgtctcttc aaacaaaaac cgcccaagtg ataagaaacg ggaaagaaga
    42181 aacgttagcg attgaagaag tagtcgttgg tgatcaacta gtcatccgtc ctggtgagca
    42241 gattccagca gatggtcgaa ttatttcagg aagcagtgcg atagatgaaa gtatgcttac
    42301 tggtgaaaat cttcctgtag aaaagaatcc ggatgacacg ctttttggcg ggacaatcaa
    42361 tacgaatggt ctgctacaca tggaagtcac acaagtcggc aagcagacag tattagcgca
    42421 aatcattcaa atggtggaag atgctcaagg gagcaaagcc ccaatccaaa aaatcgcaga
    42481 tcggatctca gggatttttg ttccgatcgt attagtcatc gcgtttatca cgctcatagc
    42541 tacaggtttg atcactggtg attggcaact cgcattgatc catagtgtct ctgtattagt
    42601 cattgcgtgt ccatgtgcat taggtttagc gaccccaaca gctattatgg taggaacggg
    42661 tgttggtgca cgaaatggaa ttttaattaa aggcggagaa gcattagaag cagctgctca
    42721 cttagacagc attgttttgg ataaaactgg aacgattaca gaaggaaagc ccaaagttac
    42781 agatctagtc ggttcaaaag aagtcctatc tattttttat actttagaac aagcttctga
    42841 acatccacta ggtaaagcaa tcgtggaata cggaaaactg caagaagcag caacatacga
    42901 tatgattgat tttacagccc acccaggtgc tgggatcagc ggtacgatta atggagtacg
    42961 ctattttgcg ggtacacgta aacgattaat tgaattgaac ctatcatttg atgaatacca
    43021 agaacacgcg ttagaattag aacagcaggg aaaaacagtc atgtttttag ctgatgaaaa
    43081 gcaagtgatc gggtggattg ccgtggctga tcaaatcaaa ctagaagcca aacaagcaat
    43141 caagcagctc cagaataaag gattagatgt ttttatgctg acaggagaca acaaactggc
    43201 agccgaaacg attggtaaac aagttggtat tgatccaaag catatctttg ctgaagtact
    43261 tccagaggat aaagcagcct acgtagaaaa acttcagaaa gatggcaaaa aagtcggtat
    43321 ggcaggcgat ggaatcaatg atgcaccggc cttagcatta gctgatgtcg gtatggcaat
    43381 gggaagtgga acggacattg cgatggaaac agccgatgtg acgctgatga atagcagttt
    43441 ggcatccatt gcacaaacaa tcgagttgtc taggttgaca ttgagaaaaa tcaaacaaaa
    43501 tcttttctgg gcttttgtct ataatacgat tggtattcct tttgccgcac taggcttttt
    43561 gaatccaatc attgcaggag gagcgatggc gtttagttcc gtcagtgtat tgttgaactc
    43621 cctgagctta aatcgacaca tgaaaaagta agagaaggga tggagtaaaa atgaatggga
    43681 acgatatgga gaaaaaacat gagcataaaa aagaagtagt aaacaaagaa agtacagaat
    43741 ctcatgaaaa acagatggag atgaaacatg accatagtca gatggatcat tccatgcaca
    43801 tgggacataa ccatggagat atagcaaaca gcaaacaaat ggaccattcg atgcacatgg
    43861 atcacgaaca cggcggcatg gatcattcca tgcacatggg gaatttcaaa caaaaatttt
    43921 ggctatcgct tatcctagct atcccaatca ttcttttttc accaatgatg ggaatggaat
    43981 ttccatttca agtaacgttt cctggttctg attggctcgt attgatctta gcaactatac
    44041 tatttatcta tggcggtcag ccatttttaa gtggagctaa gatggaattg aaacaaaaaa
    44101 gtcctgctat gatgacactg atcgcgatgg ggattacagt tgcatatatt tatagtgtct
    44161 attcgtttat cgcaaatctg ctcaatccac atacacacgt catggatttc ttctgggaat
    44221 tggctacttt gatcgttatt atgctactag gtcactggat tgaaatgaat gctgtgtcga
    44281 atgccagcaa tgctttacaa aaactagcag aattactgcc agaatcagtg aaacgcttga
    44341 cgaaagatgg aaaagaagag acagtttcat tgaaagaagt aaacgaaggt gatcgtttga
    44401 tcgtccggtc tggtgataaa atgccaacag atggtgtaat cttgaaaggt gaaacaattg
    44461 ttgacgaatc agctgtgact ggcgaatcaa gaggaatcaa aaaacaagcc aatgacaaag
    44521 tgattggcgg ttctatcaat ggggatggaa cgatcgaaat cgaagtaact ggaactggtg
    44581 aaaatggtta tctagcgaag gttatggaaa tggtgcgaaa agcacaagga gaaaaatcca
    44641 agctagagtc attatctgac aaagtggcaa aatggctgtt ttatgtcgca ttaattgtgg
    44701 gtatcctggc attcatcgcc tggttgttct tgacagatct tcctaatgca ttagaacgta
    44761 tggtaacagt ctttatcatc gcctgtcctc atgctctagg tttagctatc ccattagttg
    44821 ttgctcgctc cacatcaata gcagctaaaa acggattact tttgaaaaac cgcaatgcac
    44881 tggagcaagc caatgatcta gatgtgatca tgctggacaa aacaggaaca ctaacagaag
    44941 gaaaatttac tgtaacgggt gtcgaagtac ttgatgatgc gttcaataaa aatgaaatcc
    45001 ttcaatatct aggagccttg gaagcgaatg ccaatcatcc attggctgtt ggcattatga
    45061 attatttgaa agaacatgaa atcaagcctt atcaagctga aaatctaaaa aatctttcag
    45121 gagtcgggct agaggcaaca gtcaaaaaac aacaagtgaa aatagttaat gaaaaagaag
    45181 ttgagcgtct cggattaaac gtagaacaag cattattgaa accttaccaa gaacaaggaa
    45241 ataccatcag cttcctgatt ttagaaaatc atctagctgc aatcgttgct ttaggtgacg
    45301 tagtaaaaac agaagcaaaa gaatttatcc gaacattgaa agaaagaaaa atcactcctg
    45361 tcatgcttac tggtgacaat aaaaacgctg cccaagcagt agcagattat ttagggatcg
    45421 aagaatatta tggcggttta ttaccagatg ataaagaagc tgttgtccag aaataccttg
    45481 accaagggaa aaaagtaatc atggtgggtg atgggatcaa tgatgcgcca agtttagccc
    45541 gtgcttcaat cgggatggca atcggtgcag ggacagatat cgcaatcgat tctgcggatg
    45601 tcgtattgac aaacagcgat ccaaaagata ttttgcattt tcttgatcta gccaagcaaa
    45661 cgcgaaagaa aatgatccag aacctctggt ggggggcagg ttacaatatt cttgcaattc
    45721 cgctagcagc cggaattctt gcgccaattg gtatcatatt aagtccagca gttggtgctg
    45781 tcttgatgtc gttaagtacg gtagttgtag ctctaaatgc tttgacattg aaaatcaaat
    45841 aataatgaaa aaaggttgtg acaaaaaacg tgtcacaacc tttttttcga gtaatcgata
    45901 ctcaatcagc tggttctcgg cattccagaa gtagattctc cagaaatttt atcttatttt
    45961 ttgaagcggc tcccttttgt cagaattttt ttgtataccg tttttcttat tatacgcgtt
    46021 atttctaaat aggttttttt atcgaatttt accggatctg cttgtcgtcc atatatttct
    46081 gcttcatatt gagtagttaa tcgaatgaaa cgcagatcta gcttgaattc ttgttctatt
    46141 cgcaggctat attcctgcaa agattctgcg ggtttgcgcg gtaccaatgt ttccaaaggg
    46201 ttaagaagtt tttgatatgc aatcgtatta gtagaagcaa aaaaatgaag atagataaga
    46261 taacttgtcc agcgcagaga ttttctgaa
//
LOCUS       DANJMA010000020        46219 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000020 DANJMA010000000
VERSION     DANJMA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            160..840
                     /locus_tag="PWU31_001265"
     CDS             160..840
                     /locus_tag="PWU31_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1083822.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="PWU31_001266"
     CDS             930..1964
                     /locus_tag="PWU31_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL1083823.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="PWU31_001267"
     CDS             1978..2313
                     /locus_tag="PWU31_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083824.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="PWU31_001268"
     CDS             2297..4777
                     /locus_tag="PWU31_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083825.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="PWU31_001269"
     CDS             4833..6392
                     /locus_tag="PWU31_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1083826.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="PWU31_001270"
     CDS             6389..7753
                     /locus_tag="PWU31_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1083827.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="PWU31_001271"
     CDS             7924..8994
                     /locus_tag="PWU31_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083828.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="PWU31_001272"
     CDS             9009..9677
                     /locus_tag="PWU31_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083829.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="PWU31_001273"
     CDS             9697..10275
                     /locus_tag="PWU31_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1083830.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="PWU31_001274"
     CDS             10676..11587
                     /locus_tag="PWU31_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1083831.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="PWU31_001275"
     CDS             complement(11965..12882)
                     /locus_tag="PWU31_001275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL1083832.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="PWU31_001276"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="PWU31_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL1083833.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="PWU31_001277"
     CDS             13579..14127
                     /locus_tag="PWU31_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL1083834.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="PWU31_001278"
     CDS             14242..16602
                     /locus_tag="PWU31_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL1083835.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="PWU31_001279"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="PWU31_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL1083836.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="PWU31_001280"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="PWU31_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL1083837.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="PWU31_001281"
     CDS             19108..20007
                     /locus_tag="PWU31_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083838.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="PWU31_001282"
     CDS             20000..21262
                     /locus_tag="PWU31_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083839.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="PWU31_001283"
     CDS             21423..23321
                     /locus_tag="PWU31_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL1083840.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="PWU31_001284"
     CDS             complement(23651..24370)
                     /locus_tag="PWU31_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL1083841.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="PWU31_001285"
     CDS             24707..25039
                     /locus_tag="PWU31_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083842.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="PWU31_001286"
     CDS             25026..27029
                     /locus_tag="PWU31_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL1083843.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="PWU31_001287"
     CDS             27048..27518
                     /locus_tag="PWU31_001287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL1083844.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="PWU31_001288"
     CDS             27539..28123
                     /locus_tag="PWU31_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL1083845.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="PWU31_001289"
     CDS             28138..29124
                     /locus_tag="PWU31_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL1083846.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="PWU31_001290"
     CDS             29114..29425
                     /locus_tag="PWU31_001290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL1083847.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="PWU31_001291"
     CDS             29447..31228
                     /locus_tag="PWU31_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL1083848.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="PWU31_001292"
     CDS             31221..32597
                     /locus_tag="PWU31_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL1083849.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="PWU31_001293"
     CDS             32604..33239
                     /locus_tag="PWU31_001293"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL1083850.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="PWU31_001294"
     CDS             33269..34624
                     /locus_tag="PWU31_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL1083851.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="PWU31_001295"
     CDS             complement(34816..36210)
                     /locus_tag="PWU31_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1083852.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="PWU31_001296"
     CDS             36302..37273
                     /locus_tag="PWU31_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL1083853.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="PWU31_001297"
     CDS             37270..38052
                     /locus_tag="PWU31_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL1083854.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="PWU31_001298"
     CDS             complement(38221..38592)
                     /locus_tag="PWU31_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1083855.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="PWU31_001299"
     CDS             38726..39169
                     /locus_tag="PWU31_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL1083856.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="PWU31_001300"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="PWU31_001300"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL1083857.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="PWU31_001301"
     CDS             complement(40040..41407)
                     /locus_tag="PWU31_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1083858.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="PWU31_001302"
     CDS             complement(41391..42221)
                     /locus_tag="PWU31_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL1083859.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="PWU31_001303"
     CDS             42384..42821
                     /locus_tag="PWU31_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL1083860.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="PWU31_001304"
     CDS             42897..43517
                     /locus_tag="PWU31_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL1083861.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="PWU31_001305"
     CDS             43706..44212
                     /locus_tag="PWU31_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL1083862.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="PWU31_001306"
     CDS             44229..45128
                     /locus_tag="PWU31_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL1083863.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="PWU31_001307"
     CDS             45215..46099
                     /locus_tag="PWU31_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL1083864.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        15236 a         7977 c         9766 g        13240 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaatttt
//
LOCUS       DANJMA010000021        44940 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000021 DANJMA010000000
VERSION     DANJMA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            212..604
                     /locus_tag="PWU31_001308"
     CDS             212..604
                     /locus_tag="PWU31_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1083865.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWU31_001309"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWU31_001309"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL1083866.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWU31_001310"
     CDS             3165..4775
                     /locus_tag="PWU31_001310"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL1083867.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWU31_001311"
     CDS             5067..5936
                     /locus_tag="PWU31_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL1083868.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PWU31_001312"
     CDS             6101..7372
                     /locus_tag="PWU31_001312"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1083869.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLITTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PWU31_001313"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PWU31_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL1083870.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PWU31_001314"
     CDS             8763..9026
                     /locus_tag="PWU31_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL1083871.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PWU31_001315"
     CDS             9471..9920
                     /locus_tag="PWU31_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1083872.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PWU31_001316"
     CDS             9977..11395
                     /locus_tag="PWU31_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1083873.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PWU31_001317"
     CDS             11395..12255
                     /locus_tag="PWU31_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL1083874.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PWU31_001318"
     CDS             12429..13202
                     /locus_tag="PWU31_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1083875.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PWU31_001319"
     CDS             13349..13714
                     /locus_tag="PWU31_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL1083876.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PWU31_001320"
     CDS             complement(13862..15070)
                     /locus_tag="PWU31_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1083877.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PWU31_001321"
     CDS             complement(15089..15367)
                     /locus_tag="PWU31_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083878.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PWU31_001322"
     CDS             15521..16216
                     /locus_tag="PWU31_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1083879.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PWU31_001323"
     CDS             complement(16658..17455)
                     /locus_tag="PWU31_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL1083880.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PWU31_001324"
     CDS             complement(17452..18636)
                     /locus_tag="PWU31_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL1083881.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PWU31_001325"
     CDS             complement(18661..19908)
                     /locus_tag="PWU31_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL1083882.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PWU31_001326"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PWU31_001326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL1083883.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PWU31_001327"
     CDS             21888..22202
                     /locus_tag="PWU31_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL1083884.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PWU31_001328"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PWU31_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL1083885.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PWU31_001329"
     CDS             22887..23531
                     /locus_tag="PWU31_001329"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL1083886.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PWU31_001330"
     CDS             23546..23875
                     /locus_tag="PWU31_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL1083887.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PWU31_001331"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PWU31_001331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL1083888.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PWU31_001332"
     CDS             24863..25687
                     /locus_tag="PWU31_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL1083889.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PWU31_001333"
     CDS             25680..26027
                     /locus_tag="PWU31_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL1083890.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PWU31_001334"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PWU31_001334"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL1083891.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28180
                     /gene="dinB"
                     /locus_tag="PWU31_001335"
     CDS             27077..28180
                     /gene="dinB"
                     /locus_tag="PWU31_001335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL1083892.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPL"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWU31_001336"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWU31_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL1083893.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWU31_001337"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWU31_001337"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL1083894.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PWU31_001338"
     CDS             31657..32451
                     /locus_tag="PWU31_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1083895.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PWU31_001339"
     CDS             32441..34483
                     /locus_tag="PWU31_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083896.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="PWU31_001340"
     CDS             34764..34913
                     /locus_tag="PWU31_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL1083897.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="PWU31_001341"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="PWU31_001341"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL1083898.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="PWU31_001342"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="PWU31_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL1083899.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="PWU31_001343"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="PWU31_001343"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL1083900.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="PWU31_001344"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="PWU31_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL1083901.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="PWU31_001345"
     CDS             39330..40412
                     /locus_tag="PWU31_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL1083902.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="PWU31_001346"
     CDS             40412..41215
                     /locus_tag="PWU31_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL1083903.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="PWU31_001347"
     CDS             41285..42025
                     /locus_tag="PWU31_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1083904.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="PWU31_001348"
     CDS             42036..43040
                     /locus_tag="PWU31_001348"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL1083905.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="PWU31_001349"
     CDS             complement(43085..44929)
                     /locus_tag="PWU31_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083906.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14453 a         7837 c         9348 g        13302 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat tactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt tgagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgccggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatga gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
//
LOCUS       DANJMA010000022        38151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000022 DANJMA010000000
VERSION     DANJMA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..940)
                     /locus_tag="PWU31_001350"
     CDS             complement(<1..940)
                     /locus_tag="PWU31_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1083907.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKD"
     gene            1184..2635
                     /locus_tag="PWU31_001351"
     CDS             1184..2635
                     /locus_tag="PWU31_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL1083908.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2984..3616
                     /locus_tag="PWU31_001352"
     CDS             2984..3616
                     /locus_tag="PWU31_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL1083909.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4204..5643
                     /locus_tag="PWU31_001353"
     CDS             4204..5643
                     /locus_tag="PWU31_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1083910.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5938..6156
                     /locus_tag="PWU31_001354"
                     /pseudo
     CDS             5938..6156
                     /locus_tag="PWU31_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6405..6620
                     /locus_tag="PWU31_001355"
     CDS             6405..6620
                     /locus_tag="PWU31_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083911.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6837..7093)
                     /locus_tag="PWU31_001356"
                     /pseudo
     CDS             complement(6837..7093)
                     /locus_tag="PWU31_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7280..8191)
                     /locus_tag="PWU31_001357"
     CDS             complement(7280..8191)
                     /locus_tag="PWU31_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1083912.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8210..9214)
                     /locus_tag="PWU31_001358"
     CDS             complement(8210..9214)
                     /locus_tag="PWU31_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL1083913.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9211..11334)
                     /locus_tag="PWU31_001359"
     CDS             complement(9211..11334)
                     /locus_tag="PWU31_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1083914.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWFPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11339..13786)
                     /locus_tag="PWU31_001360"
     CDS             complement(11339..13786)
                     /locus_tag="PWU31_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1083915.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13773..14162)
                     /locus_tag="PWU31_001361"
     CDS             complement(13773..14162)
                     /locus_tag="PWU31_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1083916.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14222..14725)
                     /locus_tag="PWU31_001362"
     CDS             complement(14222..14725)
                     /locus_tag="PWU31_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL1083917.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14738..14962)
                     /locus_tag="PWU31_001363"
     CDS             complement(14738..14962)
                     /locus_tag="PWU31_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083918.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(15066..16976)
                     /gene="ltrA"
                     /locus_tag="PWU31_001364"
     CDS             complement(15066..16976)
                     /gene="ltrA"
                     /locus_tag="PWU31_001364"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL1083919.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(17722..17859)
                     /locus_tag="PWU31_001365"
     CDS             complement(17722..17859)
                     /locus_tag="PWU31_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL1083920.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17856..19040)
                     /locus_tag="PWU31_001366"
     CDS             complement(17856..19040)
                     /locus_tag="PWU31_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1083921.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(19299..19589)
                     /locus_tag="PWU31_001367"
     CDS             complement(19299..19589)
                     /locus_tag="PWU31_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083922.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(19579..20721)
                     /gene="dcm"
                     /locus_tag="PWU31_001368"
                     /pseudo
     CDS             complement(19579..20721)
                     /gene="dcm"
                     /locus_tag="PWU31_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(20797..20961)
                     /locus_tag="PWU31_001369"
     CDS             complement(20797..20961)
                     /locus_tag="PWU31_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083923.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20954..21628)
                     /locus_tag="PWU31_001370"
     CDS             complement(20954..21628)
                     /locus_tag="PWU31_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1083924.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(21706..23046)
                     /locus_tag="PWU31_001371"
     CDS             complement(21706..23046)
                     /locus_tag="PWU31_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL1083925.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(23129..23464)
                     /locus_tag="PWU31_001372"
     CDS             complement(23129..23464)
                     /locus_tag="PWU31_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL1083926.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(23474..23848)
                     /locus_tag="PWU31_001373"
     CDS             complement(23474..23848)
                     /locus_tag="PWU31_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1083927.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23861..24175)
                     /locus_tag="PWU31_001374"
     CDS             complement(23861..24175)
                     /locus_tag="PWU31_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1083928.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(24288..27515)
                     /gene="fss3"
                     /locus_tag="PWU31_001375"
     CDS             complement(24288..27515)
                     /gene="fss3"
                     /locus_tag="PWU31_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL1083929.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            28303..28626
                     /locus_tag="PWU31_001376"
     CDS             28303..28626
                     /locus_tag="PWU31_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083930.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            28790..29707
                     /locus_tag="PWU31_001377"
     CDS             28790..29707
                     /locus_tag="PWU31_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1083931.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            29700..30482
                     /locus_tag="PWU31_001378"
     CDS             29700..30482
                     /locus_tag="PWU31_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1083932.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            30483..31163
                     /locus_tag="PWU31_001379"
     CDS             30483..31163
                     /locus_tag="PWU31_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1083933.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            31156..32052
                     /locus_tag="PWU31_001380"
     CDS             31156..32052
                     /locus_tag="PWU31_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1083934.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            32264..34186
                     /locus_tag="PWU31_001381"
     CDS             32264..34186
                     /locus_tag="PWU31_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL1083935.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            34187..34633
                     /locus_tag="PWU31_001382"
     CDS             34187..34633
                     /locus_tag="PWU31_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1083936.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            34649..34963
                     /locus_tag="PWU31_001383"
     CDS             34649..34963
                     /locus_tag="PWU31_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL1083937.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            34989..36038
                     /locus_tag="PWU31_001384"
     CDS             34989..36038
                     /locus_tag="PWU31_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL1083938.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            36050..>38151
                     /locus_tag="PWU31_001385"
     CDS             36050..>38151
                     /locus_tag="PWU31_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1083939.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKP"
BASE COUNT        11621 a         7360 c         6972 g        12198 t
ORIGIN      
        1 catcttttgt gacgtaactc aaaaatactt ccattggtgt ccgatagtgt aatgattttc
       61 taggaatatg atttcgtttc gatgccaccg attggataaa ggtttcagat acaccattaa
      121 aatccatagc tttatacaaa ccatctttac gtaataagcc atttgaatgt tcattcaatc
      181 ctcgttgaga aggtgttcct ggatccgcaa aataaattga aatatcgttg gtattactaa
      241 tctttttcca atttgagaat tcttttccac aatcaaatgt gatggattta aataagtttt
      301 ttgggacaca acgaaaccat tgattcagcc tattttcaat gtctttagcc tgtctgcctt
      361 cgggtttcaa ggtaatgatg actttagata gccgttcaac taatgtgatg accgcgcttt
      421 tatgttttcc acccacgata gtgtcacctt ccaggtgacc aaattcatga tcaaactggg
      481 tatagtctgt ctttctgtct cggattgacc ggcgaaaccc ttgttttcct cttttttctt
      541 tatgaccgtt tggttttctt tttcccttca ttggtaaatc gtgagtgtca aaggttttat
      601 ctttaaacat tcgatacagt gtgcgtaccg aacaagcaat aggaaactct gctcgaccaa
      661 taaggacatc aggagcccac ccttgaacaa cctttttttg aatgtattcc tgttgctcat
      721 caggtaaaac aatcggatgt cttccgcagt tacgtttgtt tttcttatat tgtttatagt
      781 agctcattgc gcttccacca gatttaagaa aacgatagac cttataaata gtttgtcgag
      841 aacgttgtaa cagattggct acttttgaaa caggttgtga ttgattgaaa taagcttcta
      901 tcattacgag ttcagttggt gtaagatggt tataggtcat ttgtactcac tccttataat
      961 tctttcgtcg gaactatttt gagtgtagca caaatgactt tttttagttg tctagcttaa
     1021 ttttacaatc ggcgttttaa aggcagtaac aggattaaac attgaaacat aatcgttact
     1081 aaatgaaatc aagaaatata ctgtattact caattcattg aaaatattta tcataatttt
     1141 taatagaaaa cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aatctttaaa
     1201 acttgcatca gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt
     1261 ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag
     1321 taattccgat gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag
     1381 tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa
     1441 tgccgagtct tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc
     1501 aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag
     1561 tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa
     1621 aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga
     1681 atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga
     1741 caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg
     1801 tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag
     1861 tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta
     1921 taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac
     1981 gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa
     2041 attattaact aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa
     2101 tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca
     2161 atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga
     2221 agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat
     2281 aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac
     2341 acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta
     2401 ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca
     2461 ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta
     2521 tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag
     2581 tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag
     2641 gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata
     2701 ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat
     2761 cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat
     2821 gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa
     2881 ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt
     2941 aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg
     3001 tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg
     3061 tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctaacaggta gaaccaaaag
     3121 ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt
     3181 tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga
     3241 atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga
     3301 tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg
     3361 attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg
     3421 tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac
     3481 tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat
     3541 agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg
     3601 ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc
     3661 gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca
     3721 acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga
     3781 tatataaaca tctataatat attattcata ctattgatat ttagtataat cctatggaat
     3841 tgaatatcat agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata
     3901 aatatgaaaa aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc
     3961 acactattaa tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag
     4021 cgaattatca ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt
     4081 acatgaggat atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg
     4141 ttgggcagta gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct
     4201 attatgtata gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt
     4261 gctatatcta tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta
     4321 cctaccatct cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt
     4381 tatctgatga ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg
     4441 aaaatcaaag tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg
     4501 ttcaatcatt cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt
     4561 atgactatgg ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt
     4621 cgggcattgg gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt
     4681 ggcggcttga ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg
     4741 ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc
     4801 catacaatcg atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga
     4861 gcaatctttt tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta
     4921 atttcagtga ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg
     4981 atttctttcg gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg
     5041 attttttctt ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc
     5101 gggttgagtc ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc
     5161 ggggcgcctt taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta
     5221 ggcttgggct tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct
     5281 ttgtggctat acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc
     5341 cccaataata cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg
     5401 atgaattcct ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc
     5461 ttatatcaag cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg
     5521 ccggatgtct ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt
     5581 gttgcctttt tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc
     5641 tgaaaaaacc actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat
     5701 ggatgatgga agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa
     5761 tactagtgct caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa
     5821 ccgcaagcta ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg
     5881 agccaacgct ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac
     5941 ttctgtcaaa aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa
     6001 tgtagacaca cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc
     6061 cgtatacatc ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa
     6121 tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa
     6181 taagagatac cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt
     6241 agtcatgaga ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt
     6301 tctaattata ataagatgac cactacagta acctactgaa atatataaac agccactgta
     6361 gtaccatttt caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg
     6421 cggatatgaa tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag
     6481 gtactggtat caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa
     6541 agctgaatct tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg
     6601 agattgaaat atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa
     6661 cgatccttgg tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt
     6721 ctttactaac aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac
     6781 ttttatctaa gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt
     6841 atcagacgtg agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga
     6901 gcataatatg aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt
     6961 ctgaaattct ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt
     7021 tacaatgttt cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg
     7081 tatctaattc catggtatat caaccatttc ctatatagac tgattatgac atggattaaa
     7141 ataaaaaggc actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc
     7201 acctatagag gatgggcgac ttctgcctga aataagttac actacttata acttctaata
     7261 aaattagatt gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta
     7321 atactaattt gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct
     7381 ttgtactcac tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa
     7441 actttaagtc aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt
     7501 tttcagaagc tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg
     7561 ttgttttggc atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt
     7621 ctggctttcc aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa
     7681 agattgtcac tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt
     7741 gctccactaa aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta
     7801 cagattgaac ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga
     7861 gaccgttttc tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt
     7921 tatttttcca tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag
     7981 tattcactac cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg
     8041 tgaaattttt ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca
     8101 ccatttttcg atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt
     8161 ccttttgatt ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc
     8221 ggccagctcc gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg
     8281 gatctcctgc atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag
     8341 taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat
     8401 caatatgagt catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt
     8461 tggcataaca ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac
     8521 caaacacata ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt
     8581 tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc
     8641 cataattata ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt
     8701 tttgaccacc tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc
     8761 cgtttttccc gacatgatct agatacccgc caccatagtt ataggcctga ataatactat
     8821 cttgatcaca ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt
     8881 gtttgatgga tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt
     8941 gcataacgtc ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa
     9001 tatactctgt tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata
     9061 cttcttctga gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt
     9121 cttcaccaaa caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag
     9181 ttaatgaaat aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg
     9241 acgatggttc attctttttt gtactttttg attggtctgc tttggcagag tggacctctg
     9301 tttcacttgt tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat
     9361 ttcttgacta gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt
     9421 tggtgcttct tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat
     9481 attcgccctt ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc
     9541 cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc
     9601 ttggaacagt ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg
     9661 gagattgtat ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt
     9721 atctttcacc aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat
     9781 tcgggattgt ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc
     9841 gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga
     9901 ttttgattgt tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc
     9961 tcgtcccaca tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg
    10021 catgacgcgt cttcccattg actggctatc attactctgg aggttgaaca tactcataat
    10081 gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa
    10141 aaatggatag gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat
    10201 caccaaagta acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt
    10261 tcctaaactt tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc
    10321 aaagataatg aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa
    10381 ggaaatgcca atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt
    10441 ggtaatactg agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg
    10501 atctttgcct ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc
    10561 aatttcttct atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat
    10621 cgaaaacaaa ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg
    10681 tacaacgatc tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt
    10741 gatttttgtg atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat
    10801 cacaaaatta atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata
    10861 cgccacatac acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga
    10921 ggtctggagt ccattttcag taataccagc caatgtttgg atattctttc caatactttc
    10981 agccgtatcc gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt
    11041 cgacaaatac aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac
    11101 gcttttccct aagccatcac cccaattcca aggaaaccaa tcccaagagc tatccacaaa
    11161 gaaatctagt tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt
    11221 atcatcaact agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag
    11281 agtgagtatg ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc
    11341 actccattct ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga
    11401 ctggatgtac ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat
    11461 tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt
    11521 ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat
    11581 tattcttcaa gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg
    11641 cattcatagc ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt
    11701 gaagaaaact ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga
    11761 taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg
    11821 tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga
    11881 tatttaactg cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat
    11941 gggcaaaatc ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg
    12001 agccttcttt tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa
    12061 ccgtccgaat ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg
    12121 ttaaaaaggt cagaatatca attgctagac tttcagaatc cttctttttc tccataatca
    12181 cataaggatc aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt
    12241 catccgcaat ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa
    12301 tcacagcttg tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact
    12361 ttccgccacc gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga
    12421 ccccttgtga ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac
    12481 caaagtaaat gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga
    12541 gaaaatcaga cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga
    12601 attcttcatg tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat
    12661 cataaaaatc tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta
    12721 ctcgaaccac atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca
    12781 attcatctac ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat
    12841 aggcatggtt gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct
    12901 ttttgttcgt cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt
    12961 gctggtaata gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg
    13021 ttaagtacgc tacatatgat tcatgattct catgttccat gcataagtac cggggctttt
    13081 cttcaatcaa acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact
    13141 tcagcttttt ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat
    13201 agatatgttc aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc
    13261 gagccaattt actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg
    13321 ataaggaaac aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc
    13381 cggagaagaa agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt
    13441 tgaagccaat aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct
    13501 ctgtctgtaa atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt
    13561 ttgatcgttc ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct
    13621 tgccttcacg attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt
    13681 caggtgacaa aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct
    13741 cttgattaaa gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt
    13801 accagagtaa tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa
    13861 gtgaccttaa atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta
    13921 ccgtcaaacg ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt
    13981 aaaaaggccc cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc
    14041 aaggataaaa caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca
    14101 ttaattgcat aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc
    14161 atcaaatcat tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt
    14221 gttaatatgg atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca
    14281 agtctctacc acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa
    14341 aatcaccagc ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat
    14401 aatagataat atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt
    14461 gaatctcaga agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca
    14521 accgattaat ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat
    14581 gaattgcata ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat
    14641 cgacaggtgg ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg
    14701 caatataaac acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa
    14761 acaaactgag taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca
    14821 aagcaatcac caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac
    14881 caatgaccac aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata
    14941 tggataggta agttcctgat attataaata tctcaaactt ttccccccat tcacacggag
    15001 cgtgcgactt tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag
    15061 tagacctata tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt
    15121 atgagctgga ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt
    15181 ttagcgtgta ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca
    15241 tttccaccag ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat
    15301 tcttccagag ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct
    15361 gcgtattcta ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta
    15421 tcaacactta gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa
    15481 tacacctttg ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc
    15541 tcgccagcaa cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta
    15601 agttgtctgc ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag
    15661 atagccatta cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga
    15721 taataatttt gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt
    15781 ttcccttttg agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt
    15841 ttgaattgtt tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt
    15901 ttaatcttga aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt
    15961 tcttgtgata cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct
    16021 ttttcagcac tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata
    16081 tacatttcct ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta
    16141 ccgcttttat tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt
    16201 tctacccaat ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc
    16261 ccttgcggag ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct
    16321 tttaatatcc tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc
    16381 tgttttatca gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc
    16441 acataatgta gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact
    16501 gacctattag gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct
    16561 aatacttgtt ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt
    16621 ctggttttac catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca
    16681 tgtggactgc ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg
    16741 ccaatatctt taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg
    16801 atatttcgat atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg
    16861 aaaatatctc cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa
    16921 tattcagcgt gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg
    16981 cttcactcct ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa
    17041 acaacataat acttttctaa tatatacacg acttgagggc attcctccat tctcattaca
    17101 agaatttctt aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg
    17161 tcagtttatt ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata
    17221 catatatatc cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta
    17281 tatggcattt cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt
    17341 tgcatttcta caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata
    17401 gaacattcta accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc
    17461 tggatagttt tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct
    17521 ttaccgagct tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag
    17581 gcgtttcaga gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca
    17641 aactttttcg ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa
    17701 ttttgtccga aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc
    17761 acttgcattc atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa
    17821 aatatctttt acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc
    17881 atactttgtt gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata
    17941 tcctggatat aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg
    18001 gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa
    18061 gttaattgaa tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc
    18121 cagttggttc gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg
    18181 gaaaacgtgg tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca
    18241 cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc
    18301 cctaacttga tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg
    18361 agactgccga tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc
    18421 ccggaacgat aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt
    18481 ttcgttagct caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt
    18541 ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga
    18601 gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct
    18661 ttatcttctt catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag
    18721 gagtaaaagg cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga
    18781 ataatttttc gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc
    18841 aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata
    18901 gatgcggtta cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc
    18961 gtagccaatt tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa
    19021 tctaaattcc gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg
    19081 agttaacacc tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc
    19141 ttacttattt tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc
    19201 ggctaacgcc gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc
    19261 cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt
    19321 acttccaact ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg
    19381 tccgcaatcc gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc
    19441 tcattcatta aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt
    19501 aaccgtcgca attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa
    19561 atttctccaa tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat
    19621 atcaaaaatg accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga
    19681 attgacttga gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc
    19741 tctaggtgtt agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt
    19801 tagtaaggta tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa
    19861 agccagattc acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat
    19921 catgattttt gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata
    19981 aactcgatta ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct
    20041 tgctggcagt gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga
    20101 accatagact tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat
    20161 gttttgatca gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc
    20221 tcgagaatgt ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt
    20281 aagcacctac cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa
    20341 cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg
    20401 tcggagaaga gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa
    20461 tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc
    20521 cactggtccg agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac
    20581 ctccttactt gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca
    20641 aaaaccgata caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc
    20701 gaataaatct aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca
    20761 ttttgctctt ggctcataca ttattaattt gtcctactat tttttattta atccaactaa
    20821 tttccgttgt ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt
    20881 tgcagtagca ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt
    20941 tacttgttct ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg
    21001 gtaaaaataa ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat
    21061 cttttattat gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct
    21121 ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa
    21181 tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg
    21241 tcactcgaaa atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa
    21301 aagttgcatc cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta
    21361 ccaaccaaga agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact
    21421 gggtgcgaaa gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa
    21481 tggcaacctc tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa
    21541 cttcggggga tttccctttg ataatataag aaataaactt atccttattt gcttttgcat
    21601 actctaaata agaatcatta ctttccatat taattatccc ctttcttcct actatataaa
    21661 agtattatac gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt
    21721 ttctaattta ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa
    21781 ctcactaatc acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa
    21841 gaagtttttt tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa
    21901 agccacgcga aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct
    21961 ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag
    22021 cttattcaaa atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc
    22081 atcaaagatt aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta
    22141 gttagacatt tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac
    22201 acaagcagaa atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc
    22261 taaatctgca tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc
    22321 gataatggtg aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg
    22381 gggtaaagaa tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc
    22441 cactacttcg tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac
    22501 ataggagtct ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt
    22561 ttccaatttt aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag
    22621 taatccatct ttcattcgat aacagatttt ggggaagtaa ctgatagtct ctctggttcg
    22681 gctactattc aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg
    22741 gttctctaga atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag
    22801 aaaaaagtga atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca
    22861 tactaaatct tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc
    22921 aaattgtcta aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag
    22981 ccagccaaga acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt
    23041 gtacattttg atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat
    23101 ttcaaaaagt tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca
    23161 attgatttaa aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg
    23221 attctgcctg ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt
    23281 ctcctaacca gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac
    23341 cttgatgtat aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct
    23401 cagtcagctc gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt
    23461 ccataatctt cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct
    23521 gccgcttcaa gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa
    23581 aaacataatt ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag
    23641 caaactcgcg taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag
    23701 aggattttaa attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt
    23761 gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat
    23821 ctacgacaat accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat
    23881 caacatacaa cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca
    23941 aatggggatt cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg
    24001 cagctactac cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc
    24061 gaccaatcac cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat
    24121 aacttaactt tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct
    24181 tttcctcttt tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc
    24241 ggtcgcgcac gttggttctt aggatttact tgtatcatct catcacctta gttccggcga
    24301 ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca
    24361 ttggaatttt tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag
    24421 ccacttggaa cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct
    24481 tttggtgtta aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc
    24541 ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg
    24601 gtatctactt ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca
    24661 atcttgccag atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt
    24721 gtttcgaaag cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac
    24781 atatccacca catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt
    24841 tggatagaaa catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta
    24901 acatccttcg ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag
    24961 ttgaataaga caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc
    25021 caagttcctg ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt
    25081 ttggcttttt catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct
    25141 accacatacc agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg
    25201 gtagaatcca gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa
    25261 ctatcttctt cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct
    25321 ttgttcgtca gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt
    25381 tcggtaatgg taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta
    25441 gaacggattt ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt
    25501 aaataatccc cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg
    25561 ttataagctg tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc
    25621 gacactttaa aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca
    25681 aatttaaaga aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta
    25741 atcacggcat ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca
    25801 tcaaggctat atccttcggg cgcttttgtt tcttggcaat aatactcgtt gatagctaaa
    25861 tgtttaacgg caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct
    25921 ttcgttccct tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc
    25981 ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc
    26041 ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct
    26101 tttacgttac tggtaacagg ggctacggtt tgattggcat acttcaattc gacttttaca
    26161 ggtttgaacg tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc
    26221 aattccaacg cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc
    26281 atttcttgaa cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct
    26341 aaggaataat tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg
    26401 atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg
    26461 gtggtagtca tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg
    26521 gttacttttg taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc
    26581 acgtcttttg caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca
    26641 tcacccgatt ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt
    26701 ttgattgtgt aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc
    26761 tttttatagg cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt
    26821 tcttttgaat tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg
    26881 tttgcggcat tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc
    26941 acagtagtcg attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc
    27001 tgataatcgt caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta
    27061 ccatctgatc gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc
    27121 tgtgccacaa tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat
    27181 ttggctttat cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg
    27241 taaatcggga ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca
    27301 taatgcggaa cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca
    27361 tattgatgtg ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca
    27421 aaagtaatcg taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt
    27481 aatacacata atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc
    27541 acaaaaaaat aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta
    27601 ttgactgaat agaatggatt tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa
    27661 atatactgcc tttaaattag aacacatttt agaacacaaa catttcaaat ttaatcaaat
    27721 ataataaaat atatttacgg gaaaaccttt ataaatcaag gtttttcaaa cttattcaat
    27781 gatacgaaga aaaaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt
    27841 taattgcgat tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct
    27901 gagcgcagtt accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt
    27961 tttgtgtttt tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat
    28021 aaacaaacgg gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt
    28081 gtgacagaag tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt
    28141 ttgataaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag
    28201 aagggattgt gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca
    28261 aacggcatgt attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt
    28321 tatatggtct ggcttcagcg agaagttcca acgcatatac aaggacgact tatgtcaacg
    28381 ataacaacga ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt
    28441 gattggaaga caacgcaacc acgtctgcga gacagtctaa tttttctggg agggagtctt
    28501 gctatagtta tccttataac cggtctggta aaattactca agcttgatct gaaagaagca
    28561 aaaattttca gtgaagagga atacagagaa acgactttgg tcgaaaagaa agaatcagga
    28621 aactgataca agcgtataac aaactgcctg ataataggaa agacacgtat tgtccgattg
    28681 ttttttttga cattcaattg gaaaatttac actttcttta tacatttctg gctgctttct
    28741 ttatacccgt caactatact tcactcatca ataagaaaga aggataagta tggattttgg
    28801 aaatgaaacg gtcttgaaag caactggtat aaccaaaaaa tacggtgctg ccaaagcatt
    28861 agataaagta tcaatcgaaa tcaaacgtgg aatgatctat ggattgattg gggaaaatgg
    28921 agcgggtaag tctaccttta tgagaactat tatgggtttg atctcgattg atgaaggttc
    28981 gatcgaatta tttggaacga cggatttgca ggcagcaaga cgacgtatgg gtcaatcgat
    29041 tgaaacccct gctctttatc cagaattaac tgcaagagat aacctacgta tccaagcagc
    29101 gaacggcggt gtgtcggata gagaaatcga agacctgctg aaaatgatgc gattggaaaa
    29161 tacagggaaa aagaaagcaa agaatttctc tttaggtatg cgccagcgtt tagctattgc
    29221 aaatgcactg atcacgaatc cagaattttt gattttggat gaaccgacga atgggatgga
    29281 tccagcaggg atggcagaaa tgcgagaaat catccaacgt ttggtaaaag aacggggcat
    29341 caccgtcttg ctttctagcc acttgctaga tgaattgtca cagatcgcca ctcactatgg
    29401 tattttgcat gaaggacact tgatcaaaga attatcgaaa gaagaattgg cacaagaatc
    29461 tcgtcaattt ataaaaattg acacttcggc gacggaacaa gcagtaacgg ttctcgactc
    29521 tcttggttat agggattatt ttgtacaatc tagccgtgtt atccagcttt ttgaaggaat
    29581 tgaccaagta gcagccatca atcaagcatt ggtagaagca aaagttcctg tggatggtat
    29641 tcacctagtt gggcaaaaac tggaagatta tttcttacaa ttaactggag gcaatccaca
    29701 tgtttaatct attaactgct gaaaaaataa aagtatggaa gagccgtaaa atgtgggtct
    29761 cactaggact gatggtcgta cttccgcttt tttattcttg gaacgaatgg tatatgcata
    29821 caaaatacgg caatgaatta aaccaagcaa cggatacagt catcaacggt gcaacaggga
    29881 ttttaatggt agaaaaaatg gctgcatgga ttcttctggc tttcgcagca tttgcgtgct
    29941 tttatattgg ggaagaattt caaaacggta cgattcgcaa tacgttatct ttaggacgga
    30001 atcgaatgac ttattatgtg tcgaaacttc tcgttacgct tttgatcaca acacttggcg
    30061 tggttttgat cacgggacta gccatgcttg cttacacact tgctttcgga tttggtgaag
    30121 ttgagggaat caaaaactat ggaaattatg ttctaaaagt atttcctgta ttgcttctgc
    30181 tgacattagg gacattatct gttccagtgg ctttgacatt catcactaga agtaccagtg
    30241 tcagtttgct tctttctttt ctttatatca tgggaacggc cttcattccc ggtgttttcg
    30301 caaaaatcaa aggcttggaa tttctaacgg agtggtttac ggaaacgtgg ctcatgtata
    30361 cggactttgc gcaacaagca acttattcgc aagcaccgaa aatgattctg gtcagtctag
    30421 taacaatcgt ggtatcatca gctctaggaa tgtttatttt ccaaaaatct gacattaagt
    30481 aggtggtcct gctggaaact attttgatta tcgaagacga tgaagctgtt cattccttac
    30541 tggaggaggt tcttgaacag agatacaaac ttcttgatgc ttattcaggt acggaaggaa
    30601 aattattgtt aagcacatat cctgtcgatt tgatccttct agatctcatg cttccagggc
    30661 tttcaggtga agcattgtta gcagaaatcc gacagacatc gaatgttccg atcatcgttc
    30721 tttcagcaaa aagcgatcaa agagataaag tttccttgtt ggcagcagga gcggatgact
    30781 atgtgacgaa accatttgat atcgaagaac ttttgttacg aatcggcatc caacttcgtc
    30841 atcagacttc tgtccaagta aaagatctgt cgcaacgcat ctattacaag gaaatcttac
    30901 tggacaaaga gtcgagggat gtaaatgtga atggtgtggc tgtccatttg acaggaagag
    30961 aattcgatct gctgaagctt tttttagaac atccgaaaaa agtattttcg cgagcaaatc
    31021 tttatgaaac agtttggcaa gaaccatatt ttgatagtga gaaaacagtg aatatgcata
    31081 tttccaatct tcgagggaag ctagcaaagg cacatgctgt gtatattcat acggtgtggg
    31141 gagtggggtt tagatttgat taaatggctg ttgcttctcg ttgttatctt tatgggaagt
    31201 tttattttct tcctgcaaag ggaaatcaag agattaacta agaaaatcgc cactctttct
    31261 gaacgacaag aatatggtgg tcgtctttca gtcgaatttc gagaaaagaa tctaatggac
    31321 ctagtagatg cactgaatac atggttggat gaaaatgaac aaaaaggaca agcatttcaa
    31381 gaaatggaag aaaacatcag actatcgatc gtcggccttt ctcatgatct cagaactcct
    31441 ttgactgcta ttaacggtta tgtacaatta ctggaacagg tagaggatac ggagaaacgg
    31501 gagaagtatt taacgatcat caaacaatct gttgcaagat tactcagtat gacggatcag
    31561 ttttatgatc ttgctcgaat agagacgaag caaaaagaaa tggatctcca tacgctttct
    31621 ttttctaaag aggtggaaga agtatttttc tctttttatg aacaattttc tgagaaaaat
    31681 atcgaggtag cgttcgctga acactcgata aatacaactg tattagcaga tccgctcatg
    31741 ttgctgcgag tgctgcaaaa tatcatacag aatttattgc gttatgcaga tggacaagca
    31801 aaggtgacat tttcaaaaga ggatccattc attcgattaa cagtggccaa tcctatcaaa
    31861 ccaggaacgc aaacctctgt tgaaaaagtc ttccagcgtt tttataccga aaataccagc
    31921 cgcacaaata cggaatctag cggaatcggg ctttacctgt caaaaaaatt ggtggaagga
    31981 atgggaggag agatgaccgc tagattagac gggggcatct tctccatctc cgtcaagttg
    32041 cggagggttt aattatacaa gattagtgag aactagtaga aaaatgtatt atcttatata
    32101 tatttaatag agaaaaaagc ctgtgatata aatattgcag gcttttttat tgatttgaaa
    32161 taatcaagaa tattagaaac ttctacatta atcaagaact ttcttctgta tgaaaataga
    32221 gataataatt gtacacttaa gctgtattca ggaggtgaag ataatgggca tcgaatttag
    32281 cgaacggcaa atgaaaatac tatacttttt gattgaaaat gagcacacaa cttacaaaga
    32341 attatcagaa caattatatg tatctacgaa aacaattagc aaagatattt taattttgaa
    32401 agaaataatc caaaaaatgc cagatgtaca aatgttggtt caacctaaaa aaggcgtttc
    32461 gcttattgga aaaaaagaaa cacttcgaaa tttattatcc agtcataaaa tcaatatcgc
    32521 agactctccg gaggaccgtc taacttatgt gtttgctaaa ttattgaata ctaatgattc
    32581 tctaaagatt caaaatcttg ctgacgaatt atacgtgagc aggtcgacgg tagaaagcac
    32641 aatgaaagat gttagaaaaa gactaaacaa gcatgggatg tccttagttt cagatcgaaa
    32701 tggcgtgcgc atcaaagctg atgaaaaaag taaaagaagg ttgatgtcag aagttgtgaa
    32761 ctattattgg ggtggcttta cagcatctaa cagtaaaaca gatctcttag acttgaatat
    32821 caatttcatt ggtgaagcga ataatttctt aaataaggac atattgaaaa aagtagccga
    32881 attgcttaat cgatacatga ataagacaca attgaaattt acagattatg aatttcaaac
    32941 tttagctatc caccttgcta ttgcgataga aaggattgaa acaagcaatt tcttatcacg
    33001 tagtaataat tctgaagaag aaatctcaaa gaatacactt attttaatca aaatgattga
    33061 aaaagcattt tcaattattg tgccaataga tgaacaagct tatgttgaca ttcacattac
    33121 ttcgattgaa aaaaatgcta tgaatcacga cttagatcaa acgaagcttt cctatttaga
    33181 tatgggtgtt gagctacaag agttagtaag aattaaatta aatgaatttt atcctgatga
    33241 agaattgatc aataatctag tattacatct caatgcagca gttaaaagac tcaaattggg
    33301 agtaaatatc tataaccctt atacggacaa aatcaaatat tcttttaaaa ggtcatttat
    33361 gataagtgta gatttattag aggaaatcga agagagattc tgtatacatt ttaatgaaga
    33421 tgaaatcgct tacgtaacac tccatattca atcacttttg gatcgttaca aacctgacaa
    33481 aacgaaagtg atattagttt gtagtagcgg atatggaaca tcaaaattat tagagcaaag
    33541 aataaataac ggttttgcag atatggttga gatagaagat gtactaagca tcaatgaact
    33601 acaagataga aaagtcactg atgaacttgt tataagcacg attccgattg aaaatacaaa
    33661 ttttcctgtt attgttgtta gtcctttgat gttaggggca gatgtcagaa aagtaaaaca
    33721 gtatctacag ttaaatgaac aaacagaaga tgcttttcta aatttattag ataaacgatt
    33781 ttgctttttt acccaccaaa taaaggataa tcaagtgaac gttttaaata taattaccaa
    33841 aaaattggta aaagaaaatt acgcgaaaga agggatacta gagggagcaa tcaaaagaga
    33901 aaaactatct tcaacagcga tgaatgattt tgctatgccg catatcgaag tagattttat
    33961 aaaaaagcca gtaatctgtg tctacgttaa tccagaagga attgattggg atgggacact
    34021 tgttcattct gttttctttt ttgcacttaa tgaaaaagta aaacctgaat tgaataaaat
    34081 ttatgaaaca ttcaatgaaa tcgcttcgaa tcatgagtta ttagcaaatt taaaaacgag
    34141 ttcgtcgttt gaaaaattag tttatatatt gaaagaaggc ttataaatga tcagtgaaaa
    34201 aatcaatata aataatatag tgatagattt aaatgcatct gataaaaaaa gtgtagtcga
    34261 aaaattatcg aaattgttag agactaatgg gtttgtgtct gaccacaaac aatttcaaaa
    34321 agacgtattt gacagagaaa accatatgac cacaggaatt gggaaaaata tcgccattcc
    34381 tcatggcaaa agcctagcag taattgaatc aacagtggct gtagccaagt taaagcggtc
    34441 tgttgaatgg ggcgcattag atgatcaacc agttgatctc gtctttttgt tagcaataaa
    34501 agacactgat aaaggtacta atcatttacg tattttagca gaattatctg gaagattgat
    34561 ggatgacgag tttgttaaaa gaataaaaga ttcgagaaat aaaaaagaat tggtaaaagc
    34621 tctatcattt taaaaggagg aatttcatat gacaaaaata gtagcagtaa cagcatgtat
    34681 cgcagggatt gcccatactt atatggcaaa agctaattta gaaaagtttg cgaagaagaa
    34741 agggttagat attaaagtag aaacacaggg ggctatgggg atagaaaaca aactttcaat
    34801 ttcggatgtt tccgaggcag atgttgtcat atttgcagta gatacaaatg tttcagaacg
    34861 aggtcgcttt gacggtaaac cgattttaac agtagggacc tcagaggtag taaaagatgg
    34921 tgaagagacg attgatgaag caattgcatt aattaacaaa taaaaaatat atcgtggggg
    34981 agttactaat ggaattcaag aaacaaggaa gagaaattct aaatcatttc caaagtgggg
    35041 tttcctatat gatcccgctt gtcgtagcag cagggttact aacttctatt gcagtcattt
    35101 ttggtggcac aggtgtttgg gaccagacgg atacattctg gggagtcctg cgaatgattg
    35161 gtcagacggg attacagttt atcgtaccga tgatttcagc ctatatcgca tattcaattg
    35221 ctgaccgtcc cggtctagca ccagctttta tcactgggat gatgtgtcaa aatcttggta
    35281 tgggatttat tggaggaatg gttgctggat tagcttgtgg ctatttagct aatgcattga
    35341 aaaaagtaaa agtaccgatg caagttatta gtctaaaatc aattatgctg attccttttg
    35401 ttactacatt agttgttgga atcatattat ggtatgtact agctacgcct attcaagcaa
    35461 tgacaacagg actgactaat tggctagaag gcatgtcagg agcaaatgct gctttgatgt
    35521 cggctatttt aggaggaatg atggcgtttg atatgggagg acctatcaat aaaatcgcat
    35581 atgcttttgg ggttgcttct ttttcaagcg gaagctttgg acctagtaca gccatgttat
    35641 taggaattgc tattccgcca tttggaatgt ttttagcaac aattttaaac aaaaaacttt
    35701 atgatgaatc ggaacgagat aatgcaaaga cagctatgat tatgggcctt gtggggatta
    35761 ctgaagggac gatcccgttt gccgtgaaag atcctttacg agtaatacca agcatcgtgg
    35821 tgggcactgc agtagcatgt ggtctaaatg gattttttgg tgttactcaa gaaacaatgc
    35881 tttcaacctt tatggcaatt ccttttgtta cgaatatacc tttatattta gtatcaattg
    35941 ttattggggg agtggttaca gcgttaatgg tcaatgcact aaaatccatc aagtataaaa
    36001 aagaacaaca agtaaattca gtagaagaag gaaagtagga gaatctgata tggtaaatgc
    36061 acatataatt caacacacgc actgggacag agaatggtat tttacaaatg aagatgccat
    36121 cgtattgtct gatcaggtct ttactgaagt tctagatgaa ttggaaagga accctaaagt
    36181 aaacttttgt ttagatggac aaacttcaat cgtcgatgag tatttggaga tcaatccgca
    36241 aaagcttaag gttatccaac gactgatgaa aacaggacaa ctattcgtgg gaccgtggta
    36301 tgcacaaaca gatgccttat tagtggatgc tgaatcaatt ttaagaaact taatgattgg
    36361 aatcagtgat acgatcctta aatatggaga tccaatgatg gtagggtatt tgcctgacac
    36421 gtttgggttc aatgcgaatt tgcctacact tttgcatcaa gtagaaatcg ataatttcat
    36481 gtgttggcga ggattaaact ttgaaacgat ggttccatct ccttatttta tctggaaagg
    36541 attgggagat aaatttgttt atgccatgaa ttttccattt ggttatatga ctggaatgat
    36601 gaccttagac gcaattaaag atattcctga atttgcaaaa gaaaagttgg atgaagcagt
    36661 tgaaatgctt catatgcaag gaaaaaacaa agatatttta gtaccttcgg gtgtagatca
    36721 aaagtcaatg attttaaatt ttgatcaagt cgtagagaag ttgaataaat acagtaaatt
    36781 ccacaatatt atttcagatt atccttctta tgtagaagtt atcagaaaca aaaaagaatt
    36841 acctgaatat aaaggtgaac ttcgtcagcc ggtttactct cgtgttcatc gttctattgg
    36901 ctcagtaaga acaaaaatga aaatagaaaa ttttaaattg gaacaaaaaa ttctaaaacg
    36961 agttgagccg ctcatgctga ttgcagaaag aaatggtatc catatcagta aaggcctact
    37021 tcgtcggtta tggaaaaaga ttttgcaaaa ccaggctcat gatagtattg gtggctgtgt
    37081 ttcagataat gttgcggaag atattttcca tcgaatcaaa gaagctaatg agatagctga
    37141 cggaatcgaa aaccttattg taaaacggat ggctgatgca ttaaagctaa aatcgaatga
    37201 agtccttgtt ttcaacacaa cgactgagaa ttatagtgga tcgaaaatcg tgcatgtagt
    37261 agctgattca aagaaaatac acttcaaagg tagtccctat aatatcattg aaaaagaagt
    37321 ttattttcca gaacggaaaa atgcttatcg gatgattgct acaggtttcg aatattttga
    37381 tgaaccagct tattatgaac ttgacataaa aataaatgta aatgtgccag cactaggata
    37441 cactgttgtt gaatttgaga aaaataatga aaaattagaa acagcaatcg agatagacaa
    37501 aactgagata agtaacaata aatataaact ttcttttaaa gatgggcaat tgaatttgat
    37561 agttggagat agacaatatc ttgatttcgt tcatttgatc gattcagcaa atgatggcga
    37621 tacgtatgat tattcaccat tagaaggaga tacagaacta tctctaaaat tagaaacagc
    37681 taaagtatat aaagattctt tacaagaaac attagttgtt tacggaaaag ctcaacttcc
    37741 taaagattta aaagatcgtt tatctgaaaa acccgaaatg gaagaaatat cttatgaaat
    37801 atcgttcagt ctgggagaaa gtcaaatcgt tgaaggcaca ctcaaaattc ataatactgt
    37861 ttactcgcat cgtatgcgct tagcaattaa tgttttagat aaatctgaaa aatcaattgc
    37921 acaaattcaa aacggattta ttgaaaatac ccctgatgtc attcctccga attggtcaga
    37981 aactatggta gagaaacctg ttaatttaga aatattcgat aagagtgtct ctgtggcaga
    38041 aaatgatcat tattttacgt tttatgcaga tggtttaaaa gagtatgaac gtatagaaaa
    38101 tcaattaatc ataaccttat tttcaacaac aggggaactt ggaaaaccta a
//
LOCUS       DANJMA010000023        37148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000023 DANJMA010000000
VERSION     DANJMA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(144..2297)
                     /locus_tag="PWU31_001386"
     CDS             complement(144..2297)
                     /locus_tag="PWU31_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL1083940.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2591..3439)
                     /locus_tag="PWU31_001387"
     CDS             complement(2591..3439)
                     /locus_tag="PWU31_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1083941.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3663..5603)
                     /gene="thrS"
                     /locus_tag="PWU31_001388"
     CDS             complement(3663..5603)
                     /gene="thrS"
                     /locus_tag="PWU31_001388"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL1083942.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5940..6119)
                     /locus_tag="PWU31_001389"
     CDS             complement(5940..6119)
                     /locus_tag="PWU31_001389"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL1083943.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6166..7989)
                     /gene="cadA"
                     /locus_tag="PWU31_001390"
     CDS             complement(6166..7989)
                     /gene="cadA"
                     /locus_tag="PWU31_001390"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1083944.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8267..10546
                     /locus_tag="PWU31_001391"
     CDS             8267..10546
                     /locus_tag="PWU31_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1083945.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10639..11286)
                     /locus_tag="PWU31_001392"
     CDS             complement(10639..11286)
                     /locus_tag="PWU31_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083946.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11465..12184)
                     /locus_tag="PWU31_001393"
     CDS             complement(11465..12184)
                     /locus_tag="PWU31_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1083947.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12315..13466)
                     /locus_tag="PWU31_001394"
     CDS             complement(12315..13466)
                     /locus_tag="PWU31_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL1083948.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13683..14423)
                     /locus_tag="PWU31_001395"
     CDS             complement(13683..14423)
                     /locus_tag="PWU31_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1083949.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14427..14750)
                     /gene="rsfS"
                     /locus_tag="PWU31_001396"
     CDS             complement(14427..14750)
                     /gene="rsfS"
                     /locus_tag="PWU31_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL1083950.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14807..15400)
                     /gene="yqeK"
                     /locus_tag="PWU31_001397"
     CDS             complement(14807..15400)
                     /gene="yqeK"
                     /locus_tag="PWU31_001397"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL1083951.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15384..16034)
                     /locus_tag="PWU31_001398"
     CDS             complement(15384..16034)
                     /locus_tag="PWU31_001398"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL1083952.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16044..16355)
                     /gene="yhbY"
                     /locus_tag="PWU31_001399"
     CDS             complement(16044..16355)
                     /gene="yhbY"
                     /locus_tag="PWU31_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL1083953.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16371..17480)
                     /gene="yqeH"
                     /locus_tag="PWU31_001400"
     CDS             complement(16371..17480)
                     /gene="yqeH"
                     /locus_tag="PWU31_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL1083954.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17477..18004)
                     /locus_tag="PWU31_001401"
     CDS             complement(17477..18004)
                     /locus_tag="PWU31_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL1083955.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18184..18969)
                     /locus_tag="PWU31_001402"
     CDS             complement(18184..18969)
                     /locus_tag="PWU31_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL1083956.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18962..19831)
                     /locus_tag="PWU31_001403"
     CDS             complement(18962..19831)
                     /locus_tag="PWU31_001403"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL1083957.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19832..21211)
                     /locus_tag="PWU31_001404"
     CDS             complement(19832..21211)
                     /locus_tag="PWU31_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL1083958.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21219..21638)
                     /gene="fabZ"
                     /locus_tag="PWU31_001405"
     CDS             complement(21219..21638)
                     /gene="fabZ"
                     /locus_tag="PWU31_001405"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1083959.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21663..22139)
                     /gene="accB"
                     /locus_tag="PWU31_001406"
     CDS             complement(21663..22139)
                     /gene="accB"
                     /locus_tag="PWU31_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL1083960.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22142..23377)
                     /gene="fabF"
                     /locus_tag="PWU31_001407"
     CDS             complement(22142..23377)
                     /gene="fabF"
                     /locus_tag="PWU31_001407"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL1083961.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23396..24133)
                     /gene="fabG"
                     /locus_tag="PWU31_001408"
     CDS             complement(23396..24133)
                     /gene="fabG"
                     /locus_tag="PWU31_001408"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL1083962.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24145..25062)
                     /gene="fabD"
                     /locus_tag="PWU31_001409"
     CDS             complement(24145..25062)
                     /gene="fabD"
                     /locus_tag="PWU31_001409"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL1083963.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25169..25393)
                     /locus_tag="PWU31_001410"
     CDS             complement(25169..25393)
                     /locus_tag="PWU31_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1083964.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25446..26408)
                     /locus_tag="PWU31_001411"
     CDS             complement(25446..26408)
                     /locus_tag="PWU31_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL1083965.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26418..26858)
                     /locus_tag="PWU31_001412"
     CDS             complement(26418..26858)
                     /locus_tag="PWU31_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1083966.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27164..28129
                     /locus_tag="PWU31_001413"
     CDS             27164..28129
                     /locus_tag="PWU31_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1083967.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28186..28416)
                     /locus_tag="PWU31_001414"
     CDS             complement(28186..28416)
                     /locus_tag="PWU31_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL1083968.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28502..29389)
                     /locus_tag="PWU31_001415"
     CDS             complement(28502..29389)
                     /locus_tag="PWU31_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL1083969.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29478..30701)
                     /locus_tag="PWU31_001416"
     CDS             complement(29478..30701)
                     /locus_tag="PWU31_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1083970.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30718..31764)
                     /locus_tag="PWU31_001417"
     CDS             complement(30718..31764)
                     /locus_tag="PWU31_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1083971.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31928..33655)
                     /gene="ptsP"
                     /locus_tag="PWU31_001418"
     CDS             complement(31928..33655)
                     /gene="ptsP"
                     /locus_tag="PWU31_001418"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL1083972.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33655..33921)
                     /locus_tag="PWU31_001419"
     CDS             complement(33655..33921)
                     /locus_tag="PWU31_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL1083973.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34400..36634
                     /locus_tag="PWU31_001420"
     CDS             34400..36634
                     /locus_tag="PWU31_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1083974.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36782..37102
                     /locus_tag="PWU31_001421"
     CDS             36782..37102
                     /locus_tag="PWU31_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL1083975.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10450 a         8039 c         6480 g        12179 t
ORIGIN      
        1 gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg tatgaagtca
       61 aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac tcgaaactaa
      121 gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata cgcgtccatt
      181 atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt cggcaagaca
      241 acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt actattgact
      301 gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt tgcggaaagg
      361 tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc atggacaact
      421 tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac cgtattttct
      481 actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc attgccatag
      541 atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt tgaagcatat
      601 tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc ggtaaagtta
      661 cctggagcat accattcttt gccattatcc gcattgaact tcttcatata atcaacagaa
      721 cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc aagaccaaac
      781 tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa actaggtagt
      841 cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat catatacgta
      901 ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct tcgattaaag
      961 acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa aacttgattt
     1021 ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac tacagaccct
     1081 ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt ggaaccttgt
     1141 tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat agccactgca
     1201 taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata attccttttc
     1261 aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa attagaacct
     1321 ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc aagggaaact
     1381 tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa ataactttgt
     1441 cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc atctgctgga
     1501 agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact tgctgcatta
     1561 tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc gcgttctgcc
     1621 acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt gtttaattca
     1681 tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc gtttaaatta
     1741 atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt ccctaattgc
     1801 ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc agccagccaa
     1861 aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat cagcgagttc
     1921 agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct cgtatatgtg
     1981 atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta gatcattccg
     2041 cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc ctccatctct
     2101 tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa taacgtaaat
     2161 ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa cgttttgttc
     2221 ttaggttctt gtgcaccttt tcttattttt tctaaccaag aagtatttaa tatttttttc
     2281 ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt cattgtaccc
     2341 aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta cctaatttca
     2401 agataaaatc aactttttgt ttcacagcaa tcacttaaag ttacatatcc aactattttt
     2461 ttcattaaaa aataaactta tttttgaaaa ttatgttttc ttaagtcgtg aaacactttg
     2521 atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta tataaaaaat
     2581 agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca
     2641 ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag
     2701 caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag
     2761 atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg
     2821 caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc
     2881 tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga
     2941 gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca
     3001 gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg
     3061 attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt
     3121 ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg
     3181 cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt
     3241 tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac
     3301 tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac
     3361 cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa
     3421 tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac
     3481 catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca
     3541 acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa
     3601 tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc
     3661 tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc
     3721 aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc
     3781 aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc
     3841 ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa
     3901 cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt
     3961 gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg
     4021 acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt
     4081 tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt
     4141 atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc tagttttggt
     4201 ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg
     4261 gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta
     4321 ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat
     4381 actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca
     4441 aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct
     4501 gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa
     4561 gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca
     4621 agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata atgatcccag
     4681 tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa
     4741 cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct
     4801 tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt
     4861 tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct
     4921 ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt
     4981 gagtttccac gccaataagc acctgccact gataataatt taaatacttg tatacggcca
     5041 gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta
     5101 atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa
     5161 atttctaatg cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg
     5221 attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca
     5281 ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt
     5341 ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca
     5401 agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga
     5461 gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt
     5521 gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca
     5581 tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat
     5641 catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa
     5701 gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg
     5761 accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa
     5821 gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc
     5881 aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact
     5941 tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca
     6001 tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc
     6061 atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata
     6121 atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt
     6181 cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat
     6241 agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt
     6301 acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc
     6361 tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt
     6421 caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat
     6481 accgatatct gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac
     6541 aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg
     6601 ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc
     6661 cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc
     6721 tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt
     6781 tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc
     6841 aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag
     6901 ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat
     6961 atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc
     7021 attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc
     7081 aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat
     7141 cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag
     7201 tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca
     7261 ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc
     7321 taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg
     7381 atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa
     7441 agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa
     7501 agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc
     7561 gccaggcttg actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa
     7621 ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc
     7681 tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa
     7741 tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc
     7801 taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa
     7861 gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa
     7921 aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa
     7981 atgtgtcatt gttttttctc ctcttttatg tgacttagag aggttagtaa taaatttcca
     8041 gctgcctctt tcatcttaag catagtcctt gactagcact ttttaaaaca ataggcctta
     8101 attgaaaaca actatcacta tcagcgtttc caaaattcat cagttctaca aacaaaaata
     8161 ttcagcaagg attacttttt cgattcatca gttaaaaagt accttttttc gaaaaactat
     8221 gctatacttt taattcgtta tttacggcgg aaggagcaaa catgcgatga atgagaaaca
     8281 actagaacaa acgcacgtgg atgaaacgat cgaactaatc cagatcgaac aaaatatgct
     8341 ggatacaaaa caacaagaat tgactcagga aatgaatcat tcgacaaaag acacagctaa
     8401 tcacaagatt cgagctggtt ccaatgaagc tttttatgaa tctgctgttg aatatcgcca
     8461 acatgagcag gaattgcttt tgaaatacca tactcttgaa gcacagcaaa aacgcctgca
     8521 aacgttgtct gtcatgaaag atagtcctta ttttgcccgt atcgacttta aagaagaccg
     8581 tgaaacagaa acattatatc taggtatcgc ttctttaaga gatctggcag aagaaacaat
     8641 cgttattgac tggcgcgcac caattgccaa tctttattat gaaggtgaga ttggtcccgc
     8701 attttatgaa actgaaacag atagaataga tgtcgatctg cagttgaaac gacaatttaa
     8761 aatccaagaa ggacgtattt tatctatggt agatacgtcg gaagtaatca atgatgactt
     8821 tttgctagag atattggatg aatcatcaag caaccatatg aaaaacatcg tctctacgat
     8881 acaaaaagca caaaatgcaa tcattcggga cacaacaagt aaagtaatgc tgattgaagg
     8941 aatcgccgga agtggaaaga catccgcttt gcttcaacgt attgcttttc tgttgtatca
     9001 taatcgcaag tggctcgagg ctgaaaacgt tcttctattt tcacccaacc atttgttttc
     9061 tgattacatc tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta cacagacatt
     9121 caaaaattat ttggtgcagc tgctgccaga gttttcttta ctagatgaac agcaacaaga
     9181 aagtggtttt ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg gattgacctt
     9241 agtcgatcag attgatcgct atatccaagg catcacaaac tacggtccct tgttccgtga
     9301 tatgaaaata aatggccgta cgatcctatc aaaggaatcg attcgccaat ggtataaaga
     9361 aacaaatgaa caattacctt tacatcatag actttctcta ttacaaacga agctcctgaa
     9421 aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag atctggcgga
     9481 agaacagctc caagaactat acgcaacaga tccaaatctt gaatatacag aacaaaagga
     9541 gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc gtcgaatcca
     9601 tcgagggatc atgaattatc aatttgtcaa tataacgaaa caatatctcc actttctaca
     9661 aactgtgcca ctttctcttc taacacgaca taaaatcagc gagaccaggt ggcaggaaca
     9721 tttgacaact gttcgtacac acttgcgtca aagagaattg cgtcaagagg atgctgtact
     9781 attcttttta ctgatgcgcc gaacatatcc agtgacggtt acacacaaag ctcgctatat
     9841 ctttattgat gaaatgcagg atttcgcacc atcacaagtg gcattgctgc aagccttgtt
     9901 cccaaaagca aatttgactt tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac
     9961 gatcgttgga tcattggaag accttttcgt ggaccgtgaa gtcactcgtt tccaattaac
    10021 gacaagctat cgctccacaa aagaaatcac tgattttgct aatcatttta ttgtgaaaga
    10081 acatcaagta gaaacaacag caagaaaagg agaccttcct ttgctcatcc aaggaacaac
    10141 agatgatatc aaattgaatt ggttagcaga taccattcga acgactcagg gaaaagcccg
    10201 gtattggcgt acagcaatca tagctaaaac acaagaagaa tgcgaagctc tttatgctca
    10261 actgccagac gagttgaaac aacaagtcca gctgatttta aatgagagtg actttatgaa
    10321 gcgttccatt attttacttc cagccttttt agcaaaaggt ctggagtttg accgagtttt
    10381 cttgtgggat gtagatgcag aacatttttc ttctcagcaa gacaaattga tcctttacac
    10441 catgtgtaca cgtgcgatgc atgaattgac cctttttacc tccaataatg aaagtccttt
    10501 gttaaacaat atggatattt catcttatga gaaaaaagta ctgtaagtaa aaagacaact
    10561 gctatcatta atgttagcag ttgtcttttt atttacaata ataattgcag aaaaagtaat
    10621 tcaatcattt aaggtcattt attctatata taaatccaaa tctgtttcag gatattttaa
    10681 tttgacaagc gaaataatca ttttcagttc ttccatgttt tctggatatt ctgggtcaaa
    10741 taccttgcta tgacggccaa tcccgccatt gtcgatcaat cggtaaattc gattccattc
    10801 aatgaattgg tcaattgatt ggaaattgct gattggatag ccgccacaat ccaaaatctc
    10861 tgatacacga aatgctaggt atgcactatc gatcacttta ttttttttcg tgtctatctt
    10921 ctcatttctt tcgataagaa ttttttcagg acgatgtttc aagctccact ctaatagtag
    10981 aaagaaagcc tccatccgat actcttcttc aaataaccga aaggctgaat cagaacgacg
    11041 aaaaagcagc ggaatcagat aattttgtct ataaatcagc attggtgcgc ggtccaccat
    11101 acactctaaa taacggttca gataccctgt accgacatag tttcctaatc gatagccttc
    11161 aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa
    11221 ctcttctact ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag tgatttttgc
    11281 ttccatattc ttccccgtcc atcttccaca tacttcatat catttcacat gatacttatg
    11341 ctctcatcat accacaggtt gaattagaaa agaaaataaa tcacacaaaa aagcaagaca
    11401 atctgttagt gagattgtct tgcttttcga aaaagactga atacttttcg ttaaaactgg
    11461 ttgcttagtc ttctaatgcc acattatgga acacatgctg tacatcttct agatcttcca
    11521 gtgcatcaat cattttttca aatgtttctt gatcttctgg agatagtgtt acctcgtttt
    11581 gagggatcat ttggatctca gcgattgaaa aatcagtgat tcctttttct tttaatgctt
    11641 cttgtatact atggaaatcc gctacttcac cgtaaacgat gatttgtcct tcttcttcca
    11701 ttacatcgcg aacatcgatg tctttttcca ttaggtattc aaggatctcg tcagcgtctt
    11761 ctcctgcaaa agcaaagatt gctgtattgt caaacatgta agaaaccgct ccggaaacac
    11821 ccatgttccc gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat
    11881 tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg tccaaatcct tcatagcgca
    11941 attcagagta tgtttcatct cctgcacctt ttgctttttc gattgctcgg tcaataatat
    12001 gttttggtac attataagtt ttagcgcgat caatgacgaa acgtagtttt tggtttgcat
    12061 gcggatcagg atcacctgat tttgctgccg cgtagatttc aatcccaaat tttgcataaa
    12121 tccggctatt attagcatct ttagcagttt tctttgctac gatatttgcc catttacgtc
    12181 ccatattttc acttcgcttt ctgatttgat agtttcataa cgcaaaaacg tcctgttcca
    12241 ttatacttgt gaatcacgat tctgtgaagc tttttttctt tcttccttca ttcttctcat
    12301 ttttagtcaa ttatctatac tcggattggg atttgcccga aattctgttc cggaatattg
    12361 cctcccaatt gatagatttg atcacttcta actaacagtt ccgcttcttg tgcctctttt
    12421 tttccgattc gggaataaat agaaatctct tttttttctt ttaagaatct tcttccagca
    12481 tcggtaaatc caagtagtcg aagataattt ttattttgtt ggacaggaat ctcttgcttt
    12541 ttgatattca gcaagacata acaagataat cgttggatcc gtgtccatgt atatcttttt
    12601 gtttttacct ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg
    12661 cgcgattcta ttccttcggt catctgatag atatctgtca gttccagcaa agtagaagat
    12721 aacaaccgat aacgcaaata cggccaataa gattcccaag tctgcagcga acaagttgaa
    12781 agatcttctt ctgtctgagg cggcacaagt ccagctattc tttttccttc ttctaacgca
    12841 tggcgaatgg ctgttgcact ggctatcctc tgatctgtga tgattttttc atgatagcct
    12901 gcacttttac gtcggatcgg atgtaattcc atagggtgga catacttggc attttccttt
    12961 gcataactta atcccaagat atgatttggc gaagaaaaat caagcgtaat atcgggatac
    13021 agactgcgaa aaacctcagt cattttttgc ggataactat acgtttgatc agacagttct
    13081 tgaaatgcct cttctatcct atcttgattc tcttggacaa agcgtccgaa actgctgtaa
    13141 tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt cacagtccag tgtttgcaag
    13201 atttttactg cacctttagc aaaatagtca gctgactgaa ccgcccattc gacgggaagc
    13261 tccactacta aatccacacc attttgtaaa gcttcattcg cgcgatgcca tttatcgatg
    13321 atagccggtt cgcctctttg caaaaaattc ccgctcatta cagcaatgac gacctctgct
    13381 cctgttattt ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa aggattgtac
    13441 tctacgataa tcccgcagct tttcatgcgc atcctccttc atagttatcg ataaaaaaga
    13501 aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat ataaactatt
    13561 ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt
    13621 tcggatatat aatttatgtt gaagatttat tcttttcttg ttaactcgag caggcaaaac
    13681 cgctagtctt tgtatgctac aaagaaccag cgctttgatt tctctgatgc tgcttcgtct
    13741 gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt tttccagcat tcttaaataa
    13801 ctttccaatg gataggtacg ctcttgatgt agttcatctt cacgaataaa tcgaccagac
    13861 ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct
    13921 ggataactat cccacaaaaa agcaaaatta tccccttgat aatgatagct atattctgga
    13981 aaaacctcat ctacttgata ggtggagtgt acgtcaaata aaaatcggcc attttcttct
    14041 aacgcttgat agacttcatc aaacacttgc tgtacctctt gtctattttt catatagcac
    14101 agcgaatcag aaaaacaggt aattgcctga tattgtccga tttctgataa atcgagcata
    14161 ttgccttcca caaattggat cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc
    14221 aacatctctt ctgaaaggtc taaccctgtc actttatatc catcttttgc aaaggcaacc
    14281 gctaatgctc cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga
    14341 cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg
    14401 aaagcaaatg tttcataagc catatgttaa tccaaccatt cactaagatc gactagcgga
    14461 gcatctgacc atagtttttc cagattatag aaatttcgtt ctggcgcatg gaaaacatgt
    14521 acgatcacat ctccaagatc gatcaatacc cattttccgc cttcttttcc ttcaatacgt
    14581 tttacttcat atttgttttc ttcttcttta tcaatgattt cttctgtaat tgcgttgatt
    14641 tgacgttcgc tatttgctga acagatcatg aaataatctg ccaacaagga aacatttcga
    14701 acgtctaaag cgataatgtc ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt
    14761 aattgttctt tactatctat gatgatttcc tcctaatatg agcagcttat ttttttgcta
    14821 cccagtaatt atatgtttcg attgtttttg gataaatctg ttgttcttgc gcaagcaaat
    14881 gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg
    14941 ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc aatatagtct gctacataaa
    15001 tgattttgtc aaggagactc atcttagcag ccccagtcgt atgcacccgg attgcatgaa
    15061 gaatctctgc atcggtgatt cctaattccc gctctacaaa cgaagcgcca acaagaccat
    15121 gccagattgc attattataa ttcaataact ccggatcaaa accgtctctt acgattacca
    15181 ttttgaactc ctcatctggt cgttcttttg cataatcatg tgtcaatgca gcaatactag
    15241 ctttttcagg agaagcaccg tattttttgg caagagccac ggctgtttct tctactccaa
    15301 gcacatgctt gaagcgttgt tcgctcattc tcatctgtat tttttgcatc aggttttctc
    15361 tagaataagc agtatattca ttactatagt ccatattcat acaacccttc attttgaata
    15421 taatcaatta ctttatctgg aacaaggtag cggatagaac agccttcttt gatcttttga
    15481 cgtattttcg tggaactgat gtcgatctca ggtacatcta cccaaattac aggatatgga
    15541 gtatccgttg tgtatcctgg tcgacgtata cccacaaagt tgaccataga agtcagctca
    15601 tcgattttat accattttgg caaatattct accatatccc caccgataat aaagtaataa
    15661 tctgtatccg gattgttttg cgtcaattcc ttcatagtat cataggtgta acttttccca
    15721 ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc
    15781 aacatgttga gtcgatgttt tgcatcaatc gtctgtttct catcaacatg aggtggctga
    15841 taagaaggca tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg ttcagccatc
    15901 actaaatgtg caaggtgaac gggattgaat gttccgccca agatccctac ttgctttttc
    15961 ttctctaaga attgaggcat ttcttcagga aataattgtg aacgaacaaa tgtcttagct
    16021 tgcgtattca tcatggtcac tccttatatt gcttttactt cttttgatag cctttgatac
    16081 ttttcttttg tggaaggttt gtataataca ataacacgtc caatgatttg gacgatttcg
    16141 caatctattt ctttttctaa tacttgagct gcttcttctg caatctcatc tgtattttga
    16201 agtaacgata ctttgatcag ttctcttttt tctaaagctt cttcaatttg aacgatcatt
    16261 gctgaattga tcccaccttt tccgatttga aaaatcggct gaagatgatg cgctttgcta
    16321 cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt tccttcttcc ttaaatcaat
    16381 gctttgcgtt tcaatacatc tacacccttt ggcgcccagc ccgcaattac tactggcgct
    16441 gttacagtga tccagcctag accggcaaaa acgatgtctg ttttttcttt cacagaaaac
    16501 tcaaaacgaa caagctccgg aaatgcctcg acttcatcag gacgaggagg ctgtaataat
    16561 ccacctacat gtttttgata aaattcgtct gctttttcca gtttcgttcg atgaatattt
    16621 acatcatttg acacataagc aataaatgaa gcacgattgc cgctgataaa atcaaatctt
    16681 gcaagtccgc ccaagaaaag tgtttgccct tcattcagct gataaacttt aggtttgatt
    16741 tcttttaacg gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc catttgatgg
    16801 cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg aatctcgatt
    16861 ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta catcttgtac tcccgctgta
    16921 tttttgatga tttgattgat aagcgtcgat tttccgacat ttgttactcc gacgatatag
    16981 acatcccgtc catcgcggta ttcttcgatt ttttctaata gttccgtcat ctcatgagat
    17041 ttcttagcac tagttaataa tacatctact ggacgaattc ctgcttcgta agcacgttcc
    17101 ttcatccatt gtgttaattt agtttttttc aatgatttag gtaatatatc cactttgttc
    17161 ccaactaaaa gtactggatt gtcaccgaca aaacggtgta gtcctgggat tagtgaacca
    17221 ttgaagtcaa aaatatccac cacattgacg atcaatgcat ctttttgccc taattcattc
    17281 agtaagcgca aaaagtcgtc atccgtcaat gagacatctt gggtctcatt ataatggcgt
    17341 aatctgaaac aacgctgaca atagacttcc ccggaagcta aacctttttc caaagccgtt
    17401 tgcggcgtgt atcctaattc accgggtttg tttgtttgta taacggcacc acagccgata
    17461 cattgtaatt cttctgtcat tcgattccac ctcgccagcc catatcaggg tgttttgcta
    17521 atagataggc cataattctt ttttcccaaa aacgattaaa ttgtgtcttc caactatctg
    17581 ttgcgataat gggttttaca aggatgctac gtatccctgc acgattggct ccacgtatat
    17641 cagtcataat ctgatcgcct accattacta tctcatcatc tgctaaatcc aattgtttct
    17701 ttgctatgtt gatccctctt gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa
    17761 atttttcaat cgctcgcgcg acacgttttg aattattgtt tgaaacaaca acaacaggga
    17821 ttcctgcatt tttcatttct aaaatccaag taagcaactc ttctgttcca tctggattat
    17881 tccatgcaat caacgtattg tctaaatcgg tcaatactgc tttgatgcct agattcttta
    17941 attgggcagg tgtaatctga tagatggctt ctaccatcca tgttggtttg taatttgaaa
    18001 acatgctgtc tcctcatctg caaaaaatga aggagcacat aaagtactcc tcaaccccgt
    18061 attatactgc atatcagcaa aaaaaactac ctctatactc gttcgtcttt cagaaaaagc
    18121 atttctttac aaaatgacca aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa
    18181 caactaatat tggcggaacc tcttgtatcg tttttccagt aatttatgct gatctaatcg
    18241 aacaagttcc gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg
    18301 tggcaactgc tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg
    18361 tagttctggc gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt
    18421 ccacaaaatc gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat
    18481 ccagacttca tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat
    18541 gatggcgatg atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc
    18601 tccttcgccg cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaaggt
    18661 aatcaccggt ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc
    18721 ttcaggatgg ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg
    18781 aataccgatc actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc
    18841 gtcatctgca aataaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa
    18901 aaaatccaag cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt
    18961 cttatccacg atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct
    19021 gtttcaattc catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag
    19081 ccttttggaa atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag
    19141 caaaaccgat caagctttgg ggttctgcta gaataatatc gccttccatt gcaaaactgg
    19201 cagttactcc tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc
    19261 tatgccgttt gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt
    19321 gcatccgtgc gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc
    19381 gttcgaataa tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat
    19441 taggatccat cactccaata atgaagggaa tcccctgaat ggttgcttta ccagtgagga
    19501 ctgcttcatg aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa
    19561 atgctagcgg atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa
    19621 taatcaaagc tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata
    19681 ctttttcttc accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc
    19741 acatgttatc aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc
    19801 gaatatattt ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg
    19861 tccaattcgg taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg
    19921 caggatgact aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa
    19981 attcacttaa ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga
    20041 ttatttttgc aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac
    20101 tgtctactcg cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag
    20161 aaggagcaaa atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt
    20221 ggaatactac ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt
    20281 tttttactaa atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag
    20341 tattcatttc catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg
    20401 cattttcata attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt
    20461 ttgtttgtga gatcactaca gaaggagatt cttccagtac tttttgattg ttgcgttgta
    20521 aagaacaatc tcgctcgcct aaatgaatga catgaccaaa tcgatcacct aagatctgaa
    20581 cttctatatg gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg
    20641 ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa
    20701 ctttccgaat gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa
    20761 ttctgtctgc tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa
    20821 tgactgggac atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat
    20881 cgatcgttgc acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa
    20941 atcggctatt ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga
    21001 ttgcagcgct cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa
    21061 tgcagattgc ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata
    21121 tcgctacggt ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac
    21181 cgcggttagc tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat
    21241 tccgcttcgg ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa
    21301 gctttcactt tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta
    21361 cggaattttg ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga
    21421 gaaagtaatg caactgctcc tgcttgagcc attgcttcaa caatcaatac ccctggcata
    21481 acaggttctt ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta
    21541 gcaacgatcc gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa
    21601 cgatgtggaa tgatttcttt tatttcttgt atgttcattg ttttccctct ttcctgcttt
    21661 gcttattttt gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct
    21721 acaaggattt ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc
    21781 gcttcaataa tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct
    21841 ggtttgtcgg gcgctggttg cagataaacg attcccacga ttggcgaaac aacctcgatt
    21901 ccatcaaata ctgccgattc tttttctttc attatttctg gagcagttgt tgcttgttca
    21961 ggtatgacat tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg
    22021 cctcgacttg ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat
    22081 tccgttagcg aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa
    22141 attattcctc ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat
    22201 tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa
    22261 tctcaggatc ttgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca
    22321 gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt
    22381 tcgtgctgct gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg
    22441 cttctgcctg gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt
    22501 ccggctgtac accagcttct tccatcgcaa gcttcattgc tttagatgca ccagaaccat
    22561 ctggagtagg agctgtcata tggtaagcgt cacagtttgc accgtatcca acgatttctc
    22621 catagatttt ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacgcctg
    22681 ctccttcacc catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag
    22741 gatcagttga ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa
    22801 tcgttgcttc tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga
    22861 atgcttcccc aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac
    22921 cttttgcacc tacacgcaaa gcaatatttc cagctgccat gttggcaatc gccattggta
    22981 cgaacattgg agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga
    23041 ttgttggaag accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt
    23101 tttctttatc taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg
    23161 aaaacatatc cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt
    23221 tgacttcacc cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga
    23281 tgccattcgt cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg
    23341 aagtgactcc atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc
    23401 cgtgcatgac caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg
    23461 ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt
    23521 gcttttccgc ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga
    23581 atcctggcgc aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt
    23641 ttgttaatcc tactacgcca gctttactag ctgcataatt tgcttgacca atatttccaa
    23701 tcaacccaga tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt
    23761 tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt gatatccaga cattttttga
    23821 aatcttctgc atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta
    23881 acacatggat cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat
    23941 aatcagagat gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta
    24001 tcttttcttt tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa
    24061 aagctaaggc catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc
    24121 cttttacatc cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt
    24181 attctcaaca cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac
    24241 ttttccgggg ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact
    24301 ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt
    24361 cttcattatt tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat
    24421 ctgacttagg tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg
    24481 cccgctgaca tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc
    24541 ttcgtctact gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc
    24601 aggagatacg atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg
    24661 cgcattcatc actgcaacca ttttcccgct tccagctggg gcagcttctg tcatataagc
    24721 gcctcttttt gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa
    24781 tgccgaatat tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt
    24841 caataagcga taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg
    24901 atccaaacgt tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact
    24961 tgcttcatca aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg
    25021 atattgtgca ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc
    25081 cagaatatat gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat
    25141 gtcccagccc ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta
    25201 aatcttgaac cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa
    25261 tttcattgat gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag
    25321 tagtcaattt gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt
    25381 tttcgaatac cataataata tattcctccg atattgtttg ctttttattt ttataattta
    25441 acagtctaca actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat
    25501 attactttct gctgttttcc taaggtgatc tttccactag aaactgcttc atccaataag
    25561 atagggatgc ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct
    25621 tgcggcatct ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa
    25681 agcagataat caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt
    25741 acggcaaaat caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct
    25801 tctgaataga acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta
    25861 ccgtcagctg ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc
    25921 ccacctgctc catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta
    25981 gatagtgttt caccaccaat caccagtccc ttagttgagc cgcagcggat cattttttca
    26041 gccatgctta acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca
    26101 tgagttgctc ccaatttccc ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc
    26161 gacattgttg ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc
    26221 aatttttccg ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt
    26281 tcacgaatac ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga
    26341 tcattattag taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat
    26401 tgttccatta aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca
    26461 tccagcaagg catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca
    26521 cgatgaaaat gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt
    26581 acaacgcggc ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg
    26641 ttaatagcag tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt
    26701 gtttttttct tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct
    26761 ttgaattgag attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta
    26821 acaagataat catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt
    26881 ttgattatca aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt
    26941 aaaagatcat ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg
    27001 ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt
    27061 atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc attatttcaa
    27121 aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat
    27181 gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg gtaaggtcat
    27241 ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa
    27301 acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt
    27361 agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc
    27421 tttttataac cgacaaatga attatttcag taattataat tatgtacata ttaccaataa
    27481 gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa
    27541 aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga
    27601 aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga cttttgaaca
    27661 gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac tgacttactt
    27721 gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca
    27781 aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa agcagttttt
    27841 aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca
    27901 ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg acgtttctcc
    27961 tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga
    28021 agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtt
    28081 tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg
    28141 cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt ggattaagtc
    28201 atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt
    28261 ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc
    28321 ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag
    28381 tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc
    28441 tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt
    28501 tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg
    28561 ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag
    28621 ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt aaatctttga
    28681 taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat
    28741 tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat
    28801 caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa
    28861 tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa
    28921 atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac
    28981 cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat
    29041 ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg
    29101 tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact
    29161 gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac
    29221 tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat
    29281 cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc
    29341 gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct
    29401 cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca
    29461 taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc
    29521 gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa
    29581 aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa
    29641 acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct
    29701 tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt caaataagca
    29761 tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt
    29821 gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca
    29881 ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat
    29941 tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa
    30001 gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc ataagaaatc
    30061 ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg
    30121 ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga
    30181 atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac acgttcactt
    30241 ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat
    30301 tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa
    30361 gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg
    30421 aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac
    30481 cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt
    30541 cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact
    30601 tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg
    30661 acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta
    30721 gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca gtacactttc
    30781 gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata
    30841 accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa
    30901 ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac
    30961 tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc
    31021 ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat
    31081 gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat
    31141 tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta
    31201 ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa
    31261 accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg
    31321 ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa tagcttgaat
    31381 cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag
    31441 atagtccgct ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt
    31501 cgatccgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt
    31561 ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat
    31621 gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag
    31681 agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact
    31741 ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc
    31801 ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga caaaaacgtt
    31861 ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat
    31921 acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc gtatcgcatt
    31981 ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac
    32041 cacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta
    32101 gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt
    32161 cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt
    32221 ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat
    32281 cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg
    32341 ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac
    32401 ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg
    32461 tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga agcaatgcac
    32521 gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat
    32581 atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac
    32641 cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt
    32701 aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag
    32761 tacggtacag accaactgct tctccaccat tattatggac accaactaga tcttttggtg
    32821 ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt
    32881 ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag
    32941 ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa
    33001 tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct ggaatttcta
    33061 aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt
    33121 ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga
    33181 ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa
    33241 tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca ttatcttcca
    33301 ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta
    33361 cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca
    33421 tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac
    33481 gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag
    33541 ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag
    33601 cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc
    33661 agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc
    33721 accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat
    33781 gattgatttc aagtttactg atttaccttt atactctaag tttacatcag agttaaattt
    33841 gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac
    33901 gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg
    33961 agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa
    34021 gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc
    34081 aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac gtgtttattt
    34141 cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt
    34201 gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa
    34261 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
    34321 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
    34381 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
    34441 cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
    34501 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
    34561 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
    34621 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
    34681 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
    34741 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
    34801 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
    34861 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
    34921 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
    34981 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
    35041 gatcctattc atagatgaag ttcacgaaat agtcggtgct ggttctgctg gtgatggaaa
    35101 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
    35161 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
    35221 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
    35281 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
    35341 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
    35401 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
    35461 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
    35521 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
    35581 gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
    35641 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
    35701 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
    35761 agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
    35821 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
    35881 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
    35941 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
    36001 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
    36061 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
    36121 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
    36181 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
    36241 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
    36301 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
    36361 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
    36421 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
    36481 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
    36541 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
    36601 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
    36661 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
    36721 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
    36781 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
    36841 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
    36901 agcccctcaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
    36961 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
    37021 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
    37081 aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa
    37141 aagtaaag
//
LOCUS       DANJMA010000024        36706 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000024 DANJMA010000000
VERSION     DANJMA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..300
                     /locus_tag="PWU31_001422"
     CDS             <1..300
                     /locus_tag="PWU31_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1083976.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="PWU31_001423"
     CDS             complement(724..894)
                     /locus_tag="PWU31_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1083977.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="PWU31_001424"
     CDS             complement(1088..1267)
                     /locus_tag="PWU31_001424"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083978.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1654..2766
                     /locus_tag="PWU31_001425"
     CDS             1654..2766
                     /locus_tag="PWU31_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1083979.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="PWU31_001426"
     CDS             complement(2844..3137)
                     /locus_tag="PWU31_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL1083980.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="PWU31_001427"
     CDS             3305..3619
                     /locus_tag="PWU31_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL1083981.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="PWU31_001428"
     CDS             3640..5619
                     /locus_tag="PWU31_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1083982.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="PWU31_001429"
     CDS             5632..5958
                     /locus_tag="PWU31_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL1083983.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="PWU31_001430"
     CDS             5974..6492
                     /locus_tag="PWU31_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1083984.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="PWU31_001431"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="PWU31_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL1083985.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="PWU31_001432"
     CDS             complement(7928..9433)
                     /locus_tag="PWU31_001432"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL1083986.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="PWU31_001433"
     CDS             complement(9525..10115)
                     /locus_tag="PWU31_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1083987.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="PWU31_001434"
     CDS             10294..11940
                     /locus_tag="PWU31_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1083988.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="PWU31_001435"
     CDS             11989..12708
                     /locus_tag="PWU31_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL1083989.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="PWU31_001436"
     CDS             13187..14134
                     /locus_tag="PWU31_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1083990.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="PWU31_001437"
     CDS             14146..15066
                     /locus_tag="PWU31_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1083991.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="PWU31_001438"
     CDS             15107..16564
                     /locus_tag="PWU31_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1083992.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="PWU31_001439"
     CDS             16909..17523
                     /locus_tag="PWU31_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1083993.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="PWU31_001440"
     CDS             17516..19240
                     /locus_tag="PWU31_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1083994.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="PWU31_001441"
     CDS             19237..20688
                     /locus_tag="PWU31_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1083995.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21161..23314)
                     /locus_tag="PWU31_001442"
     CDS             complement(21161..23314)
                     /locus_tag="PWU31_001442"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL1083996.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23316..24362)
                     /locus_tag="PWU31_001443"
     CDS             complement(23316..24362)
                     /locus_tag="PWU31_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1083997.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24512..25801
                     /locus_tag="PWU31_001444"
     CDS             24512..25801
                     /locus_tag="PWU31_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL1083998.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            25814..28516
                     /locus_tag="PWU31_001445"
     CDS             25814..28516
                     /locus_tag="PWU31_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1083999.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28501..29358
                     /locus_tag="PWU31_001446"
     CDS             28501..29358
                     /locus_tag="PWU31_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1084000.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29429..30328)
                     /locus_tag="PWU31_001447"
     CDS             complement(29429..30328)
                     /locus_tag="PWU31_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1084001.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            30497..32668
                     /locus_tag="PWU31_001448"
     CDS             30497..32668
                     /locus_tag="PWU31_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL1084002.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32736..33077
                     /locus_tag="PWU31_001449"
     CDS             32736..33077
                     /locus_tag="PWU31_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1084003.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33353..35107)
                     /locus_tag="PWU31_001450"
     CDS             complement(33353..35107)
                     /locus_tag="PWU31_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084004.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35118..35954)
                     /locus_tag="PWU31_001451"
     CDS             complement(35118..35954)
                     /locus_tag="PWU31_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1084005.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(35956..36639)
                     /locus_tag="PWU31_001452"
     CDS             complement(35956..36639)
                     /locus_tag="PWU31_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL1084006.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11843 a         6446 c         7481 g        10936 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttcttcttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt ccagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ctaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttt caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt gatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctgaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa aaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct
    20821 cggtattaag ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa
    20881 tactcgaaac taaacagctt tttcacgacc tctccactaa atggtgttca atcagctgac
    20941 cctcggtatt agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct
    21001 aatactcagg tcagaacgct gatttcacaa cctctcaata aacggtgttc aaaagcttgc
    21061 atctgcggta ataagaccaa ccaaacgaaa aatatgagga tctatttttg cttggctgtt
    21121 cttattactt gatgcaggac agctttttca caacctcttg ttaattatag tttgataagg
    21181 aaaaaggcaa atcttttgct tgatatttct tagggtgagg gatgatccct aattggtaag
    21241 agatcgtgcc gcctttcagc aaatcttgat gggtaaaata taatttcgta tattctctgt
    21301 ccatccgttt gattttatgg ataaattgtt gctgcggccg attaggcgtt gcttcaatcg
    21361 tcaattcctt tccatttgac aaaagcaaaa cagccttgtc aattaatggc ataccgataa
    21421 catattcacc ggttccaggc gtgacaggat aaaatcccag actattgaag atataccaag
    21481 cggccatact tccattgtcc tcatctccag ggtaacccat tggagaatcg tcgaaggtct
    21541 gagtcatcaa ctgtttaagc agtggctgtg ccatttcagg ttgtcctata taactgaaca
    21601 agtaaggata gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg
    21661 ccatctcact catttcgtgg atctcaaaac catatcctcc tgggtcaaac gttgggggct
    21721 gattgcacag ctcaatgagt ttttctttat atctctcact gccaccatat tttccgatca
    21781 gtcctgaaaa atcgtgataa acagcaaagc tgctttgcca agcacttcct tctgcataat
    21841 cttgtcccca acgattagct gaaaatggtt ctctgaactt gccatcttta tcttttgaac
    21901 gcataaatcc tgactcatag tcaaagatat tttgatagtt caatgattgc tgctgatatt
    21961 tcttcgtcat ttcctcttct tctagtacct tggcaacttg gctaatacaa aaatcgctat
    22021 aagcatagtc taatgtgtga ttgactgatt cgtggtagtg attaggaaca tagccatatg
    22081 tcaagtaatc ttctgttcct tggcgtccat aatttggatg tttgctttga accgtagcac
    22141 ctttttccat tgccgttaga aattcaggca tcaagtctcg tcggatatct tttacagcag
    22201 catctgcaat gaccgcatcg atcaatgtcc caggcataag tccacgttca tcaggagaca
    22261 gccattttgg caaatagcca ctttctctat agctgttcaa aaaaccttcc agcatttctt
    22321 cgtatttttc tacagcaatc aaagaataaa gaggataaac cgttttatac gtatcccaaa
    22381 acccattatt tgtatagaga acaccaggtt ttatagatcg actagtcgta tcatagtgga
    22441 ttttttgccc actaggatca atttcataaa atgtttgtgg aaataaaaac acccgatata
    22501 aattatgata aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt atacgatcaa
    22561 aataggactg ccattgttct tgactgtgag caaggtaagt ctctttgctt tggttttgtt
    22621 ctagttccag gttataagct gcttgttccg agctgataaa agatgtccca aatcggatag
    22681 tttgtttttt tgtctgttca aaatacaacg ggatcgagtc gctatttttg ttggaaacag
    22741 ctaactcatc aattgttaaa ggatattcaa atctcaatat aaaataaaaa gaaaaatcag
    22801 gatcttctga accagcaaaa tgtgtgattt ttccttccac tgtgtaagga tctataacaa
    22861 agagctgatg gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa
    22921 tagataagat tccgccatac atgctaggta tcaatgtaga acagatgcca taacgtaatt
    22981 gacagatcga cagatgagtc gggcaaaata tactttcttc tgggcggtaa gaactttgag
    23041 catggaaaag ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc atatgactga
    23101 aatcacccat ccaaggactt ggttgatggg taagacggta gccttgaaaa acgcggtcat
    23161 caggatgaaa ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc
    23221 caaaaggaac gccagtataa ggctggcagt tcccattaga gaaatttggc tgattcgctg
    23281 ttgcatgtct agtatctatt tgaccgatat tcattttatt tctcctttgt tgattgtctt
    23341 ttgatgaatg aaactggaaa tttactatcg gatggtcgat cttttgtctc taaccaagtg
    23401 accaaagcat ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt
    23461 ggcgggtcaa caagagctgc tgcttggata tcatcaaatc cgataattga aaaatcttca
    23521 gggatatgat aaccatgctg tctcaagaga ttcatggtct ggatagcgac aacatcgttt
    23581 tcacaaacaa aagcagttac ttcatactta gtgatataag aaagcaattc atctaatgtc
    23641 gcgcttttgt catccagtga agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg
    23701 taaccaaggt aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa caaataagcg
    23761 atttttgaat gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta
    23821 tcggaaagaa cggtaggaaa aggtaactca tagatttttt tatctaatat gacgacaggg
    23881 aattctttca atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc aagagcataa
    23941 taaatcaatc cgtcaaattc attgattagc tcttttgctg ttttattttg tagaaagtca
    24001 agcgtcgtca tcatgacatc aatctgttta tcttgcatca cagggttcaa tcccttgcta
    24061 aaatcaccaa cagacaaatc agcgacatat ggaagaagaa acagaatccg tcgtgttttt
    24121 gtaggagaag caagaatctg ttccggagaa gcggatttaa cgaaactgcc tttcccgcga
    24181 acgcgataaa taagtcctaa ttgctctaat tcagtcaatg cacgtttgga agtgattctg
    24241 cttactttat aggtacgtga aagttctttt tcagttggta gttggctgtc tacaggtaat
    24301 tcaccagtta aaatagccgt ttgcaaatca ttaaatattg tctggtacaa tggttgagtc
    24361 atataaacac ctcttaatga tatatcatat tcaaataata ccataatgga tgaaaaagta
    24421 aataaaaaga tattttttga aagcgtagac ataattggct gtatgaagta taatctttaa
    24481 agatatatca aatagaaaga aggttttatt agtggtttat aaagatatac caaaatcggt
    24541 gcagaaattt atggaagata tcacacaaaa atgtggggat atccatgcgg actgggcaaa
    24601 gaattttaca gcagcatttg ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg
    24661 aaccaccttt ttattgacgg gagacattcc agcgatgtgg ttaagagact ctactgcaca
    24721 agttcgtcct tatttagtga ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg
    24781 gttagtcaaa aagcaatttt tttatatcaa tatcgatcca tatgcgaatg ccttcaacga
    24841 agaagctaac ggtgctggcc accaaacaga tcatacaaaa atgaacgatt ggatctggga
    24901 aagaaaatat gaaattgatt ctttatgcta cccaatacag ttggcctatc ttctttacaa
    24961 aaatacagga aaaactgatc aattcaatca atcttttatt gaaggtatca aaaaaatctt
    25021 ggacgtgttc aaaacagagc aagatcatga acactctcct tatacatttg tacgagacac
    25081 gacaagaaaa gaagatacgc tgatcaatga cgggcgaggt gcaaaaacag cttttacagg
    25141 aatgacatgg tctggtttcc gtcctagcga tgatgcttgc cgttatggat atctggtacc
    25201 atccaatatg tttgcggtgg tcgtgttagg ttatgtggaa gaaatttttc aaacgatact
    25261 cgatgataaa gaaattgtac agcaaacaca gaaattgaag aaagagatcc aagaggggat
    25321 tcagaatcac ggacgaacgg ttaatcaaaa agggcaaacg atttatgcat atgaagtaga
    25381 tggaatggga aacgccacag tcatggatga cagtaatgtg cccaatctga tcgccgcacc
    25441 ttacttggga tattgttctg aacaagagga agattatctg caaacaagaa gaacattgtt
    25501 gagtaaagaa aatccatatt tctatgaagg aaagtacgca aaaggaattg gatcctccca
    25561 tacgccagaa aaatacgttt ggccgattgc attagctatg gaaggaatga caacgtcaga
    25621 caaatctgaa aaagagcgga tattgaattt acttgtagca actgacgcag gaacacacct
    25681 tatgcacgaa ggattcgatg tgaacaatcc tgaaaattat acgagagaat ggttttcttg
    25741 ggcaaatatg atgttttgcg aattagtcat ggattacttt gatattagag tagaaaagta
    25801 ggaggtatcg taaatgaaaa agaaagtata tatcatttct catagccact gggatcgtga
    25861 atggtacatg gcttacgaac agcatcacat gcgactgatt gaattgatag acgatctact
    25921 agagctattt gaagttgatc cgtccttcaa cagttttcat ttggatgggc aaacgattat
    25981 tttagatgac tatcttcaag taaggccaga aaaaagacag gcaatccaac aagcaatcaa
    26041 tgaaggaaaa ttgagaattg gaccatttta tatcttgcaa gatgattttc ttatcagctc
    26101 ggaatcgaac gtgagaaata tgctgattgg catggaagaa agccgtaaat ggggaacccc
    26161 tgtcatgtta ggctattttc ccgatacatt tggaaatatg ggacagacgc ctcaattaat
    26221 gaaacaagct ggtatttcag cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga
    26281 taatcaagtt ttggaagcag aaaactattc ttcccaatac tcagagatgt ggtggaaagg
    26341 ccctgatcaa acagcaatct ttggtctgct atttgccaat tggtatagca acgggaatga
    26401 gattccagta gaaaaagaag cagctcttgc cttttggaag caaaaattag cggatgcaga
    26461 acaatatgcc tctacgaacc acttattgat gatgaatggt gttgatcatc aaccggtaca
    26521 aaaagatata tcaaaagcaa tccatctcgc aaatgaactg tttccagatt acgagtttat
    26581 tcattctaat tttactgact atctggaagc agttcaaaaa gatgtgccag aagacttagg
    26641 ctcagtagaa ggagaattga caagtcaaga aacggatggc tggtatactt tagccaatac
    26701 agcatctgct cgggtttact taaaacaatg gaataccaag gtccagcgac agctagaaaa
    26761 tattacagaa ccacttgcga cgatggctta tgaagttagt ggaaactatc cccatgatca
    26821 attagattat gcttggaaga cattaatgca aaatcatcct cacgacagta tttgcggttg
    26881 ttcagtcgat tctgtccatc gggaaatgat acctcgtttt gaaaaagcag atgaagttgg
    26941 aaagtatctt gcacaagatt ccttagaaca gctgaccgca gcaatcgata cgaccggatt
    27001 cccaaaagac agctttccat ttgtcattgt aaatactgca ggaatggata aaactggtga
    27061 agcagaaata accatcgaat tagaaagaaa aagattcgct gaaggaatac ctgaacaact
    27121 atatcaagag ttagaaaatt tgcctaaacg taagtatcat gtagaaacaa aaagtggggc
    27181 cactatacca gctattttat ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga
    27241 ccgtttccgt gtgccatata tggcaagaat gatcaaagtg actttaccat tagaaaacat
    27301 gccagcattt tcttgggaaa cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa
    27361 ggaaacaatg attcatcaga gtggacgaat catcgaaaac ggccctctac atttgacgat
    27421 tgaaaaaaat ggtaccatta caatggaaga tcgcaaaaac aaacgaaagt tgaataatct
    27481 gcatattttt gaagatatcg gcgatatagg aaatgagtat atctttaaac aacctttctg
    27541 tgatcagcca atcctatcca gtaacaaaga aaactctgaa gtaaaagtat tagtagatac
    27601 accggaaata gcaaaaataa gtattctaca ggaaatggaa atccctgttt ctgcagacga
    27661 gcgtttagaa aaagaacagc agatggttgt ggaatttcgt tatcgaaaag cagaacgatc
    27721 caaagaaaaa cgaatccttc aaataaaaac aatcatgaca atcagaaaag atagtaaaaa
    27781 aatcgacttt gaaacgactc tcgacaatca gatgaaagat catcgcttac gtgtcttgtt
    27841 tcctacaaag ttacatgtgg aacatcacga agcagatagt atttttgaag tggtgaagcg
    27901 cccaaaccat gtgtctaaaa gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt
    27961 tgtcaatatt catgatgaag agtacggtgt aacagtcgga aactttggtt tgaatgaata
    28021 tgaagtaaca gaagacggac aaatcgcagt gacgctactt cgcagtgtgg gagaactagg
    28081 cgattgggga tattttccaa caccagaagc acagtgtcta ggtgagcatc ggttcaatta
    28141 cagcattgaa ctacatggac cagaagaaaa attctctact tatttgcatg catacgcagc
    28201 acagattcct ttttccactc aacaaatcaa acatcatgaa ggaacattga tttcaaaaca
    28261 acaatattta acgataaaaa gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt
    28321 tagtgataaa gtagtagtaa gaggtttcaa tatgtctagt catttagaaa aattggaaat
    28381 aactaaagac aatggaaaaa ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc
    28441 ggttgtccca ataatccaac cttatgagat tcggacaatc ggattcgagg aggaaaacga
    28501 atgtatggcg gaatagaagc aggcggaaca aaatttgtgt gtgcggtttc agatggtgga
    28561 gaaattttag aaaaattaag tatccctacc acaacaccag aagaaacatt ggatcaggta
    28621 tttgaatttt ttgatcgtta tgaattgaag ggaatcggga ttggctcatt cggcccgatt
    28681 ggtatcgaca aaacaaaaga taattacgga tacgttttgg caactcccaa aaaagggtgg
    28741 gcacaattcg attttcttgg cacaatcaag gaacgctaca atattcctgc tgcttggaca
    28801 acggatgtca atgctgctgc atatggggaa ttgaagcaag gagcagcaca aggaaaaaat
    28861 agttgtattt accttaccgt gggaacagga atcggtgcag gtgtcgtgat caatgaagaa
    28921 attttttcag ggatcgcaca tcctgaaatg ggacatattt gggtgaaacg ccatccagat
    28981 gaccattatg aaggtacttg tccttatcat aaagattgtt tagaaggatt agcagcagga
    29041 ccgtctattg aggcacgcac agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt
    29101 tgggctattc aggcttatta tattgctcaa gcactgatca actatacgtt gacgttagca
    29161 cctgaaaaga ttattttagg cggtggggta atgaatcagg atcatctcct tcaaaaaatc
    29221 cgtcagcaat ttgttgaatt gatgggtggt tatatggaaa cacctcaagt aagtgaatat
    29281 atcgttcgtt ggggactgcc gaatgaaagt gggattatcg gtagtttgct gttagcagaa
    29341 cacgaacatc aaaagtaagc agacaaatta cacaaaaaga acagagtctt atcattatct
    29401 gctgataaga ctctgttctt ttttatttct attcccataa tccaggtaaa gctttaccga
    29461 ctattttttc ttgagtaaat ggatggatca tgtgcagttc taatgcatgg agcatcagtc
    29521 gttcagctgt tctattttgg tagagagggt ctccaacgat cggatgtccc ttatgagcta
    29581 aatgaactct gatctggtgt gtacgtccgg tttccaaaac acaatagact tgtgtttgtt
    29641 cagaaagtac tttgttgaca gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta
    29701 cgaccctttt tctccgatca tggcgatctc gtccaatttt attggtgatg gtcatatctt
    29761 tggcaatttc tccccatacg atggcttgat atctccgata gatcattttt tgttccaaca
    29821 ttcttccaag tatcggcaag acaaatggat tttttgcaaa taaaacggca ccgcttgttt
    29881 ctttgtccaa gcggtggaca acgtaaggtc gttgctgttt agtgtcgaga taagcagcta
    29941 aatgattgag taaggtactg ttttctgtcg gttcatttgg atgagtcttc attcctgcag
    30001 gcttatccac tataatcaaa tgctcatctt catataaaac acggatgtgc ttcgcatctc
    30061 ctaattgaac ttcttgataa ttataatctg tttcttcgaa taacaatgtg atctggtcat
    30121 tgccctttac ttcatgatgg aacaaagcag gttgttgatt gatccaaacg tttttacgta
    30181 tacgcaaaaa gtgtcgaacc tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa
    30241 ttgttgtttc aggatggttc ataggtaaag tgatcgtgat tttcacaagt gtactccttt
    30301 cagtgcaatt gcagactaat tgtaccattt ttaaaacata tttaagaaaa aacttacatt
    30361 tttctgttca ttcttatgtt actctacttg cactaacgta aaaaaacata ttgaactaca
    30421 acttaagcag gaagaaaaca ctctttcact atggtaaaat aagacgattg aatcaaatag
    30481 acaggagaag aagcaaatgg attttcaaag cattttagaa aagataaaag ctgctttgat
    30541 cagtttttgg cgttggatca gaccatatct cggacaattc catcgatggc gaaagcgcat
    30601 ctggaaaaaa tatcatgtga acaaattgat tttgctgatt ggtttgatct gtgtcttagt
    30661 gacaagtatc tacctcttta ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg
    30721 attaagccaa tcgacagtca tttatgataa gaaaaacgaa gaagctggca cactgtacgc
    30781 ccaaaaagga acatatgtag aattaaacgc gatttctccc tatatccaag atgcagtaat
    30841 ctcgacagaa gataggaatt tttacgagca tcacgggttt gatatcaaag ggattgcccg
    30901 tgcagcagtc cgaatggtct tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac
    30961 tcagcagctt gccaaaaatg cgtatctgac attagaccaa acgtttgatc gaaaagcgaa
    31021 ggaattattt ttggcaatcg agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat
    31081 gtatcttaac aatgcctatt ttggcaacgg ggtatggggt gtccaagacg cttccagaaa
    31141 gtattttgga gtagatgcaa cgaatgttac gctgtctgag gctgctactt tggcaggaat
    31201 gctgaaaggt cctggtattt ataatccgat cgatcaccca aaaaatgcga atgatcggaa
    31261 aaacacagta ctaagtgtaa tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc
    31321 agaaagccag acagatatca gcgcgtatct taatgacaca tacgataatt cagattctgg
    31381 ttatcgttat ccttattatt ttgatgcagt gatcgatgaa gcagttaacg agtatggtct
    31441 tgatgaagaa gatgtcatga ataaaggcta taaaatctat acagctttag atcaggatta
    31501 tcaaaagcaa ttagaagcta cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga
    31561 tggtgccatg gtccaatctg cttcagtagc tttagatccg aatacaggtg gtgtacaagc
    31621 actagtagga cgaagaggag atcatgtctt tagaggattc agctttgcta cacagatgcg
    31681 tcgttcacca ggatcaacga tcaaaccatt aagcgtatat acaccagcac tggaagctgg
    31741 atacaagcct tccagtatct tgaaagacga accgcaaagt tattacgatg cgcataattt
    31801 tgacgggaca taccaaggag aagtaccgat gtatcaagct gttgcacaaa gtttgaattt
    31861 gcctgctgta tggcttttgc atgagatcgg tttacaaaaa ggatatgata aagcagaaga
    31921 attcggcctg cctttagcaa aatctgataa gtattatggc ttagcccttg gtggactgga
    31981 aaaaggagta tctccattga tcatggcggg tgcttacagt gcatttgcca atgatggtca
    32041 gatgtatacg ccgcatctta tcacgaaaat cgttgattcg actggagcgg tgatcgtcga
    32101 taacacaaac aaaaaaccaa aacaggtcat atcaaaggaa acagccgatg aaatgaccag
    32161 catgcttctt ggtacatttt caaatggaac agcggctgct gcgaatcctg caggttatac
    32221 gatagcaggg aaaacaggaa ccacagaaac taatttcgat gccacaaaag caaatgacca
    32281 atggatgatc ggttacacgc cagacgtagt gatttcgaca tggatgggtt acgaagaatc
    32341 cagcaagctg cactatttgg aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc
    32401 agcggcagaa ggcatccttc cgtatacttc aaaaacagga tttagtgtag cagatgccta
    32461 tgccacagga ggacaagtgg tagctgcaga tcaagttgga aacacaggaa attctaatgg
    32521 agagtctgga aattggcaag ataacttgaa tcaatatggt gaacaagcaa aagaagggct
    32581 gaaaaacttc ggcgatatgg tgaaagaagg cgttcaggga attggtgaag cggctaaaga
    32641 cctctggcgg aaatatcaag gagagtagcc cctttgaaaa cgaataaccc catgttacaa
    32701 tagagagtaa ctactaaaag aaaagagggc ataacatgtc aaacatctac gatagcgcga
    32761 atcaaatcga acgtgagatc cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg
    32821 agaaagtaca agcaaacgaa gaagcacaca aactgttcaa agacttccaa gcgatgcaaa
    32881 tcgaattaca agaaaaacaa atgagtggac aagagttcag cgacgaagat gcaatgaaag
    32941 cacaagaaat ggctgtgaaa atccaatcag aagaagtaat caatgacttg atgcaaaaag
    33001 agcaaggctt cagtacaatc atcaatgact tgaaccgtat catcatgaca ccagttcgcg
    33061 acctttacag cgaataaaac tattttatga aacaacagag ggtgtgaaag agacgttcgg
    33121 ccttgagcga tgacaaaatt gcttcttatt gccgataggc tgtctcttga acaccattta
    33181 ccaagaggtc gtgagcctac cgtttaactc tgagttacaa ggaacaattt caaaaaacag
    33241 cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
    33301 tattcagaaa taggagctac gacaaaagtc ttgtcatagc tcctattttt tgtcattcat
    33361 ttttcaagcg gtagaaatag cctttttgag ctaacagttt ttcgtaagta cctatttcaa
    33421 cgatttttcc ttcatcgatc acaacgattt tatctgcctc tttgatcgtt gaaagccgat
    33481 gagccacaat aaaagtagtg ggtgtttctg tcaggtaatt cagggcttgc tggatttttt
    33541 tctctgactg gttatctaaa gctgaggttg cttcatctag caaaatgatt tttggctgac
    33601 gtatcaacgc tcgtgcaatg gagatacatt gcttttggcc accagacagc ttgttgccat
    33661 gttcgcctac taaggtatcc agaccatcag gaagctggtc aatcagtgaa gaaagttgcg
    33721 cagcttcgat gacttcatct aaacgttctt ttgatacgtt tttcaagcca taagtgatgt
    33781 tctctttgat cgaagcggag aacaacaaag tggtttgagg aacaacggct aaataatttc
    33841 gcacacttcg catatccagt tcatccaaag gctgatcatc tagaaaaatt tttcctgaag
    33901 tcggctggat aaatccaatc aataagttaa ggagtgtaga ttttccagag ccagatggcc
    33961 caacaaaagc aattgtttct ccagctttca cctctagaga aaaatgatcg ataatcggtt
    34021 tgagtgagtc cggataaaga aaagaaacat cctcaaaacg aaaatttcca tccaaatgtt
    34081 ggattttttt cttaccgtga taagtttcta catcgccagc aatcaacagt tcgccaatag
    34141 aagtgataga ttccatgcct tttgaaatcg taggaatcag cgtgatcatt ccagaaacgg
    34201 aattgacgat cgtcgtgaaa tacgtctgat acatcacgac atcgccgggt tggatgcgcc
    34261 cttgtaaagc taatatccca gtgaaactca aacaaatcag ctgaaatatc tgaaaactag
    34321 cccagctgac tgatccgaaa acagattgca ggatatctaa gtggaatcct ttttcataga
    34381 tttgagtaaa atgcttattt aagcgagttg tttcgtgttt ttctaaccca tgagctcgag
    34441 caatcggtgc taattcgatc acttcgatca cttttgcaga agtctcttcc atttcttgcc
    34501 tgaagtctga gttggactgt ctgatccttt tacggaaaaa gagagtaacg aatgcggcga
    34561 gaggaacagt caataagaaa aacatgaaaa caacaggact tttataaagc gtgataccta
    34621 aagctacgaa taaattgaca gcgatattta atccgctgac aaatacttga gaggacaacg
    34681 tttggatagc ttctacatca cggatgatct ttgactggat tcgtccagat tgcaattctt
    34741 tttgataagg aatagatagt tcttggattt tcttgataag ggtcgctctt agtccagctt
    34801 ctacttgacg gatcgaagtg ctatggtatt ttacatgaaa ataattcgtc aatatatttt
    34861 gaccaatcag acaagccatc aaaaaagcgt tgaaataaat ggcctgcaac tgagaagaat
    34921 ctttcgatga agctgcattg attacattgg caatagcgat cggcattaac caagatggaa
    34981 gatgtttgat aataaaaaag agtcctgaca tggcaaaacg ccaataattt ccctgataaa
    35041 gatgcagcaa tgtctgaaaa gcatttgcat gatatttttg ataacttttc tgtattcgtt
    35101 cctccataat cttctcctta acaatcttgg taaataaact gcaagtgttc tattgcttca
    35161 ggaacataaa tctcaggagt agcttcaaga gtggcgtaca ttagaggacg ctcatatttg
    35221 atataatgca gtaccgggcg gaaatctaaa cagcccttgc caacaggtac catgcggatc
    35281 tttccatttt ccatgatata gtctttgaga tgaagcgctg taatataacc atgtaaattg
    35341 tccaaggcac ctcggatcac tttttcctga tcagcatgat tttctttatg catcaaattc
    35401 gcacaatcta aaatgatttt taaatttggt gaagctactt catcgattaa acgtttagct
    35461 aactgatagt tatagattgg atgattgatc cctgcttcaa cagcaacagt tacgccaaaa
    35521 ttttctgcat gagcgactaa ttcaatgata gaatttctgg cgatctgata agcttcttca
    35581 gtaaaatttt cttttgtgta accgacagag acactaccgg tttccgtagc aatcatcttg
    35641 gcttgatagg cagagcacag agcaatttgc tgtttgaaag ttgccaatgc ttgtgcacga
    35701 acttcttgat ttttgctgga taggttgata tagcatccta aaatggaaag ttcgatattg
    35761 tttttttgga agtattcacc aaaataggca gcactcccaa gtgtcatgtc agttaattta
    35821 agttgttcag gaaatgcttt ttgtggtgca aattggatat gggacaagtg aagctgtgat
    35881 actttttgaa cgatttcttc acgatttcga acatctaaat cgtgggcacg gatgcctaaa
    35941 tttaatggaa acatctcaaa ctcctttttg taaataatta gcaagaggca gacgggattc
    36001 ttttatcaat tcaacaatta ttcgggcaac tttatcagcg ccttctggac taaagtgggt
    36061 attgtctttt agtccttctg ggtaattttt acaagtatca ggcataagat gcaggtagaa
    36121 tttttttgtc tcttcttttg agaaggtctg gaacaattct tgtgatcttg agaacacatc
    36181 taaaagagga atatggtgtt tctcagcaaa tgcacgcatc gcttttggat agtctccaag
    36241 tatatctgga ttcaatgttc cgttttctaa gtaatctcgt cgcgtcacag atgtcagtaa
    36301 gaccatttgg acgttcgctt tttttgcgca ttcaaaaaat atctgcagat tttttaaata
    36361 gctaccgaag ggaagcgtac ctctttcttc ttggattttc tggtcgttat gtccaaattg
    36421 tacaaataag aaatcattcg gctgaaactg tgaagctaga cgggctaacc ttccttcctg
    36481 gataaatgat ttggtgctgc gtccattctt tgcttggtta ttgatctgga tctcgggtga
    36541 aaaataagca gagatttttt ctccccaacc tgtctcggga cgcttgtttt catctttttc
    36601 tgccgcagta gaatcaccgg caatatggat agtaggcatg ttatctcttc tttcataaag
    36661 gtttataggc aatgaagcag aacagcaaga gaagaagacg gagcaa
//
LOCUS       DANJMA010000025        33979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000025 DANJMA010000000
VERSION     DANJMA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..357)
                     /locus_tag="PWU31_001453"
     CDS             complement(<1..357)
                     /locus_tag="PWU31_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL1084007.1"
                     /translation="MLTKKELIDHIDETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIIDILDLKVDPELGGILRGKEGYFPAYHMNKEHWISVDLRKI
                     EKLDEPDCRQKLSVRTA"
     gene            501..1940
                     /locus_tag="PWU31_001454"
                     /pseudo
     CDS             501..1940
                     /locus_tag="PWU31_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1947..4499)
                     /locus_tag="PWU31_001455"
     CDS             complement(1947..4499)
                     /locus_tag="PWU31_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL1084008.1"
                     /translation="MNWYKLTVEDTLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIVKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHTVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFSYGMDWPILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVNEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLSNMKNCQELKTKEEQKTENLSGNPTELALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHTEGKGAVIYVKGAPEVLLAKSTLSSSEQEAWGQTATAFAKK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILILALILNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVALLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKSLFLSLGILALLQGMVIFLPS
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREFSR"
     gene            complement(4612..5943)
                     /locus_tag="PWU31_001456"
     CDS             complement(4612..5943)
                     /locus_tag="PWU31_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1084009.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIALAGPVVI
                     LVYLICGVAIYGLMRAMGDLFLANSKFNSISDFIDYYLGEKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIV
                     FFILLGIGLSVSYFHTQSIGIKANFGQLNELLPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYVGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQSGAAIVNLMILVSSLSSANSAVYSTSRILYQTAKEKSLPPWFCALSKKGVPLHGIL
                     ITGTLFLAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHIQFVKEKKRNTIP
                     KYKDLALLVFFVAIAFSLLFERMTRFIPFLLVIWFVLLSFVYKRISDRNIN"
     gene            complement(5909..7387)
                     /locus_tag="PWU31_001457"
     CDS             complement(5909..7387)
                     /locus_tag="PWU31_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1084010.1"
                     /translation="MKDAGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFIVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLTFDMAKIGGKIGVWLGLY
                     VPLTMMLLLGLAAWIKVGIVPTGTLGNFSWDKVIPDSTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGISVLRLRY
                     THAKLNRPFRIGKKENKSNLGVWAIVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7694..9529)
                     /gene="tdc"
                     /locus_tag="PWU31_001458"
     CDS             complement(7694..9529)
                     /gene="tdc"
                     /locus_tag="PWU31_001458"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL1084011.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMADGWGHITADGSIANLEGLW
                     YARCIKSIPLAVKEILPEKVKNMSEWALLNLSVEEILEMTESFTDEEMDKVKAASSRR
                     GKNIQKLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     DQKIPILGVVAVVGTTEEGQVDSVDKIIQLREKLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHYVTIDKEVYEGFRAISEADSITIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVEAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLEQLHFTVKGKTIEVYPLNHPDFNMVNWVFKVQDC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFIERMGLSKEE
                     WQKEQQITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIK"
     gene            9999..11426
                     /locus_tag="PWU31_001459"
     CDS             9999..11426
                     /locus_tag="PWU31_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084012.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIVYTHREKDCYTLYFSEHIPRCKIMQQLTQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFHDCGIEIDRYSPRFTEMERRLLLLNVAYRL
                     GSFKSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQLGTVTID
                     PTFIEEIKKRPIYNYVESTWETTDFQTYYKKEEFVFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFETHFRQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQANLYKDVQTLFSSWAKRLPYDLRFNPNCMRAFVIELSGILRLTKERLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYASISDIKKYAQQPSNRVLCERTLYTPDA
                     VQYENIIPISINTIERAIISAVQNK"
     gene            complement(11587..11952)
                     /locus_tag="PWU31_001460"
     CDS             complement(11587..11952)
                     /locus_tag="PWU31_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL1084013.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLIWGVCLSIKDFLKYLF
                     VHTEAKNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVPNK"
     gene            complement(11949..12314)
                     /locus_tag="PWU31_001461"
     CDS             complement(11949..12314)
                     /locus_tag="PWU31_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL1084014.1"
                     /translation="MFHSNLIEFLEWIIAILNICSIGILVAGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEIG"
     gene            12755..12883
                     /locus_tag="PWU31_001462"
     CDS             12755..12883
                     /locus_tag="PWU31_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL1084015.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            12940..14634
                     /locus_tag="PWU31_001463"
     CDS             12940..14634
                     /locus_tag="PWU31_001463"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL1084016.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKDTPMQDEITVTGLKMTNTLTQ
                     EEEHVTVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(15038..15898)
                     /locus_tag="PWU31_001464"
     CDS             complement(15038..15898)
                     /locus_tag="PWU31_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084017.1"
                     /translation="MKQIQNKWNKRIIVMISSVCLFCLTVMLCLISTLANQHFMLEQN
                     MQSDYAKKVAAEINREIQSYHYEIGAPAEALEKIVSEELVQKNIELFIRNRYEKEPVE
                     LIGEKEVEEKLQEIINTYKQEEAEDDPFASEENNLLIYMTSGIFRRGIQSIYLMSFID
                     QILTIRERMVSFLPMISSILLVLLSGVFYLYRMSWKKVLWSFAVVIGGAGFQLLSLSV
                     SLYLYDMASQLDIRTQALEHLLDTYILSFIQFFSVVAIVEVVIAAGLWWIGKRVNTEK
                     KVFSKRNGYK"
     gene            16189..17322
                     /locus_tag="PWU31_001465"
     CDS             16189..17322
                     /locus_tag="PWU31_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL1084018.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPRILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDETNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNNLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLEEAAQAYEIFDKKEDKNIKVVLKP"
     gene            17353..17667
                     /locus_tag="PWU31_001466"
     CDS             17353..17667
                     /locus_tag="PWU31_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL1084019.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAVFLTIRPILRIVRNIFIYDEKRTRKKHGK
                     AV"
     gene            complement(17804..18262)
                     /locus_tag="PWU31_001467"
     CDS             complement(17804..18262)
                     /locus_tag="PWU31_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1084020.1"
                     /translation="MERRTVIKLRDRPTLIKETAEWFASKWHLPVAVYEESIQASTRK
                     NTSVPQWYVVLNEWGEIIAGAGVVDNDFHDRVDLTPNVCALFVEKDYRNQRIAKMILE
                     TIKQDFAEMGVKVLYLVTDHTDFYEKYNWQFIGMAQDNEGIPVRVYQMTL"
     gene            complement(18484..19737)
                     /locus_tag="PWU31_001468"
     CDS             complement(18484..19737)
                     /locus_tag="PWU31_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084021.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPVIGGVLATKSIAGGVLAAVNLI
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(19766..20083)
                     /locus_tag="PWU31_001469"
     CDS             complement(19766..20083)
                     /locus_tag="PWU31_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1084022.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LDA"
     gene            complement(20108..20428)
                     /locus_tag="PWU31_001470"
     CDS             complement(20108..20428)
                     /locus_tag="PWU31_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084023.1"
                     /translation="MEKRTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQIESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(20741..22156)
                     /locus_tag="PWU31_001471"
     CDS             complement(20741..22156)
                     /locus_tag="PWU31_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1084024.1"
                     /translation="MKITIPENFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAKIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYHSGNYGGR
                     IGCILNPEMVYARSSSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCRKQSIYF
                     DPATEDLETVQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEEQYMDEKGIVQDDYRIEF
                     IGQHLRYLIKAAEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRILKKS
                     ASWYQSLIDKRELSLPDEAYK"
     gene            complement(22173..23489)
                     /locus_tag="PWU31_001472"
     CDS             complement(22173..23489)
                     /locus_tag="PWU31_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084025.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLVTGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIVVSILIYLPFVKVANRVENDVI"
     gene            complement(23639..24337)
                     /locus_tag="PWU31_001473"
     CDS             complement(23639..24337)
                     /locus_tag="PWU31_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL1084026.1"
                     /translation="MEENKTLVEVTTDKILRYLKEKQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQISRMKSLTQKIETSFREGTQDHVQLDIDFHTTIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMATN
                     RRELQKIIGKKETN"
     gene            complement(24614..25570)
                     /locus_tag="PWU31_001474"
     CDS             complement(24614..25570)
                     /locus_tag="PWU31_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1084027.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRELLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELKIDQILE"
     gene            complement(25710..26513)
                     /gene="msrA"
                     /locus_tag="PWU31_001475"
     CDS             complement(25710..26513)
                     /gene="msrA"
                     /locus_tag="PWU31_001475"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1084028.1"
                     /translation="MDKEQVFIQTLYNLILNPNTRDWERKVLIQTKNDIRENTSAKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLLTEGFDTQQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGNLSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SDLLTIYWQITDPTDAFGQFQDRGKQYRPVIFYQDERQKKLAEQSKQELVSSGTFRQP
                     IVTKIEPVGTFWPAENYHQQFYKKQPKRYKKIQQARKQFLIYQRIKNKW"
     gene            complement(26748..27491)
                     /gene="truA"
                     /locus_tag="PWU31_001476"
     CDS             complement(26748..27491)
                     /gene="truA"
                     /locus_tag="PWU31_001476"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1084029.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNSEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHA
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLMGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(27627..28364)
                     /locus_tag="PWU31_001477"
     CDS             complement(27627..28364)
                     /locus_tag="PWU31_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1084030.1"
                     /translation="MFKYMDKIKQLLDLVEKEEKKSIKEAIDVLTQANLQKRSVYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGREVMLDRSPITFTSQMERLEGYGTNLAKTVSF
                     KNQDVLILHSVSGRNPIIIDLALTAKAKGVKIISLTNVQYSRSVTSRHSSGKRLFEVS
                     DIVIDNHGDIGDACCELTGIVQKAGPSSTVIGAAILNTIIVEVCEQLIANGIKHPPIF
                     YSANLDGGDLRNAELYEEYKEVIHYEL"
     gene            complement(28365..29615)
                     /locus_tag="PWU31_001478"
     CDS             complement(28365..29615)
                     /locus_tag="PWU31_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1084031.1"
                     /translation="MNFISEHILKNPPVLLGLIAMLGLFIQRKSFSETVKGALTAAFG
                     MVALTAGVNMLVGTISPINIAVQTQLGVEVTEGLSDVTFTAEYGGTVGLAMFLGLVIH
                     LLIARFTPVKTIFLTGHMLWWFPFVFVAAGVEAGLSGGALIGISGILSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGVLSLVSGFVAKRVGNKEKSTEDIKVPENLSFFREISI
                     TGALVMFLMNTVVGIIAPVLIPEGDNLLMFSIEAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVVLANSFHLFGVLL
                     VPLVITSFFECGGAAVIGEGQGGFRGAVVGTIAASFVMVVLAGFSAIVFSTTIQSWIL
                     IFGGNDLSLWGMIGRGFSGLFGGF"
     gene            complement(29620..29901)
                     /locus_tag="PWU31_001479"
     CDS             complement(29620..29901)
                     /locus_tag="PWU31_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084032.1"
                     /translation="MKILAVCGFGVGSSMVLKMSLDKVIKELGLKATVENTDLSTAKA
                     TMADVYFTSHELLPELKASVKAPVYPIKKYMDTSEIKAQLEQYLADKGE"
     gene            complement(29914..30345)
                     /locus_tag="PWU31_001480"
     CDS             complement(29914..30345)
                     /locus_tag="PWU31_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084033.1"
                     /translation="MSTINKLLPKNQIQVIETAENWMKAVEIASNPLLKNKMITHTYL
                     ENMIRSVEENGPYMVLADYFALMHAKPGLGVNEQSMSLLVVKDAVDMKGKPIHIFLVL
                     AAKNHESHLERLKDIMEIFMDNEKYQTILSGNKETIIQLFA"
     gene            complement(30342..31982)
                     /locus_tag="PWU31_001481"
     CDS             complement(30342..31982)
                     /locus_tag="PWU31_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1084034.1"
                     /translation="MSVIQYQESKLRRVAILNRLSNFEHVSYQQLSEEYYVSRSSIAN
                     DISYIKYVFAKEGLNLSFDRFGTYFEGNEIQVQKVLKRMILNHFDELEIVREIVDRKL
                     LAIVERSFRESIADKQIELPESYFSSIIVSILLMIQRSREGKKISLKGKNQYGKYFLE
                     FNKYPLVCELLHQLEEQRIYQFSQEEVQYLTYVIVGSGLRFFMKNETIPLVFREKIRQ
                     LIQKVSRGIQIDLTQDSRLEEDLLVHLYQLLLRIEAQTTIVNPLIDEIKQNYPSLYGV
                     VWFALNDFRWPYQVSLSEDEVGFVAIHFQAAIERVKRLNRLLFVCPNGMGTSSFVSAK
                     IRRILPDIDSIETASVDKLNRLDLFDVDLIISTVDIPKQIRPVVRISPMVTARDMKRI
                     MNHYIDLVIDNEQINIEGSIAEKVKPLISSNVYFDHFDSKEAAIRFLVDQQQFENEES
                     KQRFLYSIFEREEIQSTYLENGFAIPHGNPNVVKQTSVAVLIADKPFSWGYQKADIVV
                     LLMIREEDVKEVEPVMKMVMQGIEDKNWFISKMLEVRE"
     gene            complement(32202..32837)
                     /locus_tag="PWU31_001482"
     CDS             complement(32202..32837)
                     /locus_tag="PWU31_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL1084035.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EISSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTASIGFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWSFLNKIGFCQRISFFFNYVPIAWLSILHLRQ"
     gene            complement(32989..>33979)
                     /gene="sstT"
                     /locus_tag="PWU31_001483"
     CDS             complement(32989..>33979)
                     /gene="sstT"
                     /locus_tag="PWU31_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL1084036.1"
                     /translation="LSALVAVIISSIFTVPITLQETVSEQAPQNLESVLSTMLTNVTQ
                     NPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQISVALSQVVQVIIAFAPIGI
                     LGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLILFCL
                     KESALPAFFTRSSAANIPINMMLAEKLKLTKESYSISIPLGATINMGGAAITISVMSL
                     TAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIV
                     GIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDRLDGLNER"
BASE COUNT        10432 a         7157 c         5720 g        10670 t
ORIGIN      
        1 agctgtcctc acagacagct tttgtctaca gtctggttca tctagttttt ctattttacg
       61 tagatcaaca gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt
      121 tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaaggatgt cgatgatcct
      181 atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca
      241 ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc
      301 tggagacaaa ttgtatgttt catcgatgtg atcgattagt tcttttttcg ttagcatctg
      361 cttccatctc tttcttattg attcacttta ttcaaagata ccacgtaaga atagaaaaat
      421 gaatactggt tgaaagatca gttgaaactt gaccaccgtt tattttctcc gtattcttag
      481 aatagaaggg agtctgattt atgtattcac taatgaaaaa aatcattacc gaaaaagata
      541 ttcgacgtca tgtttcttta gtcgaacaat tgctgaatca tacaaagata acggtaaatg
      601 aattagctga aatcataggg acaaccgaac gaacgatttt ttctgacctt caatctattc
      661 gctctcagct gcccgaaggc tgggacatcc tcagcgatca agcaggaata agtttgcaaa
      721 accatcaaaa tttattaaca aatgaccttt gggagatttt tttcaaacaa tcagtcagtg
      781 ttgagctgct gaaaaatctg ctttttacaa aaaaagttgc tgtccctgat tttctagcgg
      841 atcacgggct ttcttatggg acattaaaaa gacatgtgac taaaatcaat cagcgtttag
      901 caagttacga cttgcagatc gatttgacga aatatacagc ttgtattctt ggaaaagagc
      961 gtgcgattcg tactttctat catcgattgc tgattccttt cactcataac aactattttt
     1021 tcgaggatta ttcgatccat gaatcccatt attttcaatt tttaagaaac ctcagtcaaa
     1081 ctgaactagc tgttgaaaca gaagagattt tcggaacatg ttggttcttt atcaatacga
     1141 tccgtataaa agcgaattgc cgattagatt cttctatcca tatcaacagc acactctcct
     1201 ccctgtatga ctctgcattg aaaaaattgt atttaacgga aggtatttat cttaaagaca
     1261 ctgagctttc ttttgcttct ttctgctttc tggaaagttg gaattacaac aataactatg
     1321 gacaagaaat cgctcgctgc ctgcatcatt ctccattttt agaggtcctc gaaacgtttg
     1381 tagaggaact agcttcagag ctgtctcttg atcaactaaa aaaaacatct ctgactgata
     1441 acttggctct cctcgtatta aaatatcatg aatcgccgat actgatttaa cctttggaac
     1501 gccaatacca tcgtttttta gagacgtatg atcagcaata tgcacacttg tatcagcaga
     1561 aagagaattt actgaacgat ctaaaaaaag aaatggaaat caatgaacca aattactttc
     1621 ttcaactctt gtctttactg atccaacaaa cgattttttc tatcaaaccg aatttgttca
     1681 atgtctattt tattttccaa ggagaacctt catggaaggc gttcttgcag caggaattaa
     1741 aagattactt gggaaaacgt gtctgcttct tacctatcga actaaccgat ctttccacta
     1801 tttcttttga aaaaacagat atcttgattt ccaatttccc tctggattct ctagatatcc
     1861 ctattgtata catttcttct attccaacga aaaatgagct caatcgtttg actgaactca
     1921 ctttcaaaac ttttttatag taacggctag cgactgaatt ctctagctgt tttttctttt
     1981 ttgcgaaaca gtttattcat gatccctttt tctatttcta caactaagaa tagaagaatc
     2041 acatttgccc caatcaataa ctgttgtgtc atcgttaaac ttactgtacc gatcagctgc
     2101 tggccgcttg gcagaaaaat aaccatcccc tgcaataaag caaggatacc caatgaaaga
     2161 aaaagcgatt tgttttgcaa aagaccttgg ttcaacgacg gcttggacaa ctctctgcaa
     2221 ttgatcatgt aaacagcttg agctaagacg atattttgca gaagaatggt ttgctgtaag
     2281 ctttgccctt caaattgcat agccaataaa taggcaggaa ccatgatcag tagtgcgaca
     2341 tagatgatcc gaaacaagtg ataacctgat aggattcctt gctttggatc tcttggtgga
     2401 cgcttcatga tttctggatc tggcgactcg aaccctaatg cataagataa agtgattgtc
     2461 gttaccatat tgacccataa aatctgtact ggggacaaag gtagcggccg attcagtatc
     2521 agcgccaaaa tcaagatcaa tccttgagct aacgcagttg gtaagaagaa attgatggtc
     2581 ttttgcaagt tatccaaaat gcgccgacct tcttttacgg cttttgcgat cgtatggaaa
     2641 ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc tttgattccc
     2701 atagcgattc ctatatcggc tcttttcaaa gcaggtgcat catttacgcc gtctccggtc
     2761 attccgacga tttctccatt tttctgcaaa gctttcacga ttcgcagttt gtgttctggt
     2821 gtcgttcgag caaagacatc gacttttggt acttgtcgaa tcaactcttc ttcagacata
     2881 agatccaaat cgatcccttc taaaacttta gcagtatgtt ttaagccaat ttgttccgca
     2941 atcgcttttg ctgtgtcttt atgatccccc gtaatcattt tcactgagat ccctgcttgt
     3001 tggcattcct ttacagcctt gactgcactt tcttttggcg gatcgatcaa cccggcaatc
     3061 cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga atctactttt
     3121 ttgtaagcaa atcctaaaac acgctgacct tttttcgcaa acgctgtggc tgtttgaccc
     3181 catgcttctt gttctgaaga agaaagagtt gatttagcca ataatacttc tggcgcacct
     3241 ttgacgtaaa tcaccgctcc ttttccctcc gtatgcatcg ttgccatata tttgtattca
     3301 gagctgaacg gaatctttgc ttctattttt ttcaaaggca gattggctgt ttcagtgtgt
     3361 cgaatcagtg caagttctgt cggattgcca cttaaatttt ctgttttctg ttcttctttc
     3421 gtctttaact cctgacaatt tttcatatta gacagtacgc gagtttcttc ctttgctgct
     3481 acatccacaa ctgtcatttc attttttgtc aatgtccctg tcttatctga acagatcact
     3541 gtcatagaac ctaatgtttc tacagaaggc agtcctttga tgattgcctt ttcttttgcc
     3601 atttcattta ctccgacaga taggatcatg gtcaaaacag ctggcaatcc ttcgggtacc
     3661 attgacacca ccaaagcgat catagctgaa agaaggatag gccaatccat gccatagctg
     3721 aatgttgtaa aaacaactaa gaacaagatc aaacaaagaa tgccttgaaa aatttgtttg
     3781 ttcaatcggt tgatcttctt gatcagaggc gtcgtttgtt gttcgaccga ttgcagtgct
     3841 tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc gactgcagaa
     3901 ccagattgta caagtgttcc tgaaaaagcc atatttttct ggtcaccgat tgattcttct
     3961 gctgccaaaa cagtatgatt tttctcagat gcttctgatt ctcccgtcaa aatcgattct
     4021 tccactagta gatcatgtac ttcgatcaac cgaacatctg ctggaacaac gtctccagca
     4081 cgtaagacaa tgacatctcc ttttactagt tctttcgcag gaattttttc ttgcatcccg
     4141 ttcgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa cgattcttct
     4201 gcttttcttt cttgccaata actaacgagt ccgttgacga tcactacgaa taaaataata
     4261 cttccttcga tgtattcgct cgaagcaaat ttcaaaaaag aggcaatgat caacacgatc
     4321 atcagcagat cagtaaagtg ctttactatt tttttccatc gttttgtctg ctgtcttgct
     4381 tctatctcat tatatccttg atcagtcagg cgtttctgcc gttcttcttg cgacaagcct
     4441 tctgttgatg tttgatagtt tttcaatgta tcttctaccg tcaatttata ccagttcatc
     4501 ctatcccctc ctacttaatt gatactttta ttatacgtag ggagatcttt caggcgtatc
     4561 gattatttca gagaagtctg aaataattcg ttttgccgga tatatttagt cttaattgat
     4621 atttctatcg cttattcttt tatatacaaa acttaagaga acaaaccaga taactaataa
     4681 aaaaggaata aaacgtgtca ttctttcgaa taacaagctg aaagcaatgg ccacaaaaaa
     4741 taccaacaaa gcaaggtctt tatattttgg tatagtgttc cgtttctttt ctttaacaaa
     4801 ttgaatatgt gcacataaga tcgaagacca cacaaataga aaacaactcg ttgtcatacc
     4861 tgccaaaagc tgaaaaaaca atcgactatc agcgacaaaa aaatgtagac aaagacctgc
     4921 aagaaataac gttcctgtaa tcaaaatacc gtgtaaaggt acgcctttct tagaaagcgc
     4981 acaaaaccaa gggggcagac ttttttcttt tgctgtttga tataagatcc tgctagtact
     5041 gtaaaccgca ctatttgccg aagacaaaga agaaaccaga atcatcagat tgacgattgc
     5101 tgccccgctt tggcaatgga tcatttgcaa catagtgact aaaggacttt cttttaaatc
     5161 taaacaatgc catggaacaa cgaataaaat cgacagaatc gttcctacat agaaaaagag
     5221 gatacgccaa ggcagacagt tgattgcatt tttcaaattc ttttgtgggt cactcgtctc
     5281 accaactgtt aatccgatca tttcaattcc agcaaaactg aataaaacca taggaaatga
     5341 agcaatcatt ccagcaaagc caaacgggag taattcattg agctgtccaa aatttgcttt
     5401 gattccgata gactgggtat gaaaatatga gacgctcaaa cctataccca ataaaataaa
     5461 aaaaacaatc cccaaaatct tgatgattga gaaaaaagat tccaattcgc caaaccattt
     5521 gacggcaaca agattgactg ccattaagat cattaatgca ctacctccgc ttatcaaagc
     5581 tggtacatgt acaaaccaga aatggatata caatcctatt gctgtcaatt ccgcaagtcc
     5641 agctccaagc cagcaaagcc aataagtcca attgacaaaa aaggcccact tttcacccaa
     5701 atagtagtca atgaaatcac tgatagagtt aaatttacta tttgctaaaa acaaatcacc
     5761 catagcccgc atcaagccat aaatagccac accgcatatc aaatagacga gaataacaac
     5821 tggtcctgcc agtgcaattg cctttgctga acctaagaat aatcctgttc caattgcgcc
     5881 acctagtgcg attaactgga tatttcgatt agttagagtc cgtttcattg ccggtttttc
     5941 catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc caagaaggtt
     6001 tctttatttt ttcaatacat agtggtacta caaacaaaat gacactgatg gctgtgactg
     6061 caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaag ccgattgccg
     6121 caaataaaac aataacgatc gcccagacgc caagattgct tttgttctct ttttttccaa
     6181 ttctgaatgg acggttcaac tttgcatgtg tataacgtaa tctcaaaacc gagatcccca
     6241 taatgacata gacgatacaa tagattacag tagttgaatt aactagcatc aaaaatgctt
     6301 gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg acagattgag
     6361 tcaaaataac ggatttggat acgtcaaatt gatttgtctt ccaaaaattg aattttggtg
     6421 gatacgcacc ttttctagca ctttctgtca ctgtctttgc aggtcctgat gcccaagcag
     6481 ataattggac ggccacaccg atgaagacaa gcaaactaaa cagattcaca ataagatgcg
     6541 gcaaaccgag aatttggcaa taaatcgaaa tagattgtgc gatattattc aattccattt
     6601 gaccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg aaaaatataa
     6661 aaatcagagc agcaaatact ccgcgaatat acgttttcac tgggttcttc aatcgagtga
     6721 tatacacgga ggacatttcg atgccagtaa aaataaacat gatcggcgca aaataaacaa
     6781 atgaggatgc agtcgtacta tctggtatta ctttgtccca tgaaaaattg cctaatgttc
     6841 cggttggtac gattccaact ttgatccacg cagcaagccc taataagagc atcatcgtca
     6901 aaggcacata tagacctaac caaacaccga tttttcctcc gatttttgcc atgtcgaatg
     6961 ttaaattcaa aaaagtgatg atccaataaa caactaaaat aacaactaat gtaaatttgc
     7021 tgttcagccc aagcggcaca ttatcgataa tattaccaaa caacggagcg agtgctgaag
     7081 caaccatcac catccctgga aacatttgga cccataacag ccaggagaca atgaatcccc
     7141 atttttttcc tagagcattc gatacccaaa gttctggacc acctttttga ggaaacattc
     7201 cggcaaattc accagagatc aatgagatcg gaaaggcata taacaatacg gcaaccagca
     7261 tgtaaaaaat catcgtccag ctcgttgctg ccacatctgg aatatttcgt acacttgcta
     7321 caagggcaca ggtcatcccg ataaaggtca tggtcgtgat ttgttttgat gaaccagcat
     7381 ctttcatctt tattccttct ttctttcaat agcgacatgt ttttcgtttt cttttatttt
     7441 tgttcaataa caaaagtgac aaggcttcat cgattcagta caaaacccag tatcaccgaa
     7501 acatccattg aacgaactta gtgaactggg tttgatacgt gaaacgaacg aaccaactat
     7561 agaaagattt ataaaaaaga atgtcttgct gaggaatata tgaaaaacag ggggaaatac
     7621 aactgttttg atgaggcaaa taattgtctg ttctccatat gtctcttgct attctttcac
     7681 acaaaaatca gttctatttg atgatttcgt ttagtttctt ttccattgct ttggcgattt
     7741 cttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg atagccgcac
     7801 gtagcagagt gatttgctgt tctttttgcc attcttcttt cgatagaccc attctttcaa
     7861 taaaacggat cggagaatca ccgtaatctt cttgtgtaaa tgtcgtatga gaagtaataa
     7921 accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt ttttcattta
     7981 attcattgat agcgttcaaa tctgtacaat cttgtacttt aaatacccaa ttgaccatat
     8041 taaaatcagg atgattcagt ggatagactt cgatcgtttt tccttttaca gtaaagtgta
     8101 attgttctaa aaattctctg aatctttgag ctgcttcgat tgatgcacca atcaattgac
     8161 catatccggt cacattcaat ggcaatacac gatgagctgt ccagactgca gcagccgtcg
     8221 ctcctgcttt tgagccttct aaaatataag cacctagcat atctggtgct tcgactgatt
     8281 tttcaaacac atatggtgca aaataagaaa tgatatttct catattttta tgtttgatta
     8341 cgattcctcc agcagcataa gggacatagc ccatcttatg cggatcaatc gtgatagaat
     8401 ccgcttctga aatagctctg aatccttcat acacctcttt gtcgatcgtt acgtaatggt
     8461 ggaatacatg atgctcttca aagaattcag ctaaggaagc atatggcaca aattctcctg
     8521 cttcgtttaa aaacagtgaa cgcgcgtacc cgccatatgc agcgtcaaca tgtaaataga
     8581 aatagatacc ttcatctttt aacttttctc ttaactggat gattttatct acactgtcta
     8641 cttgtccttc ttctgttgtt cctacgacag caaccacacc tagaatcggg attttttgat
     8701 cagctaattc acgaattgtt ttttccaaag cgttgatatc cattcgataa tcctcttgaa
     8761 ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc ttcatccaag
     8821 aatagtgttt tgtttgagga accagccatt tccctaattt ttggatattc tttcctctac
     8881 gagaagaagc ggctttgacc ttatccatct cttcatccgt aaagctttcg gtcatttcca
     8941 aaatttcttc aacagacaga ttcaaaagag cccactcaga catgtttttc actttttcag
     9001 gcagaatttc tttgacagct aaaggaatcg atttgatgca acgggcgtac cataatcctt
     9061 caaggttagc aatagaacca tctgctgtaa tatgtcccca tccgtctgcc atgttgaata
     9121 gtgaggcaaa atcctgtcct acttctgctt ccatctgcga agttgccata gaagattcta
     9181 gtgcaacgtt attgggattc catagcatcg cataattgta agctaataaa gcaggcatca
     9241 gtgtttcagc gttcatttgt ccccagtaac ggcctgctga atgccagggg atcgatcctg
     9301 cgcgtaatcg ctgactgact tcatccaaga cttctatcat atggtcgcgg gtggctagat
     9361 aatctggagt tagcttatct ccttcgctaa ttgcaggcat atctgaagga atataatttt
     9421 ctctccagcc aagatgttca tctaccatct tgttcaacaa cattttgtaa actggtccat
     9481 tttcagcttt gtcgcctata aagactgcct tgatatccat atctttcatg tttcttccct
     9541 ccatatttcg ttggtcaatg tctcgcagat acatgtgctt atctgtaaca cctcttcact
     9601 atagcgccaa aaatgagaaa atttttttta taacatcaga ttcacgtaga atatttcaaa
     9661 caaaaataaa taaagtcgga tttttctgat aattgaacgt ggaaaaacga acaatttttt
     9721 aataaatcag acttctaaaa tggatagtct aaaaaaaacg aacaaataac ttaaatgacc
     9781 atcattcatt catcaactgt atttgcattc cttttgcttg ttagaaattc acaatctatt
     9841 ttctaatgaa aatatttatt tttcaatttt tataacataa aaatctttca ttaacggaat
     9901 ttacaggatt tttcagaaat taattgatga tttccaaaag aaaacatgtc ataatatttt
     9961 ccatattgtg caaaaaaata caagatggag gctttactat gattgaagaa tatattgaga
    10021 agaatatttt gagacagctg tttttatgtg gacaattcta tgtaaataaa gaagtcaatc
    10081 tagaaaagct ctccaatctt ctacatgttt gcaaaaccac tttactcaat gatatcaata
    10141 acataaaaaa agaatttgaa gaacaaattg tttatacaca tcgggaaaag gattgttaca
    10201 ctttatattt ttcagaacat atacctcgat gtaagatcat gcagcaatta acccaaaatt
    10261 cgttgttttt aaaaacttgc ctgctttatt tggaagaaga tgaacctgat tatctccaat
    10321 taactgagtg tgaattcatc tctgtcagca aagcttactc cttaaaaaaa caagtattgg
    10381 cttattttca tgattgtggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
    10441 aaagacgtct gttgcttctt aatgtggctt atcgtttagg cagctttaag tcctgggagc
    10501 tacctgaatc gttttttgaa cgtgcggatc gtttcattga atctgtaacg gagaatagcg
    10561 gtagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct attagtttct
    10621 tgcgccaaca gttgggtact gtgacaatag atccaacatt tatcgaagaa atcaaaaaac
    10681 gtcctattta caattatgtc gagtcaacat gggaaaccac tgatttccaa acctattata
    10741 aaaaagaaga atttgtcttt attttgacac tgttcaatct ttgtaattat ggtttccatt
    10801 cttatcagtt gattgcggaa gattttcaac agctacacca agtgtttatt gataatactc
    10861 ccgaaataaa agaactagtt gctacctttg aaactcattt ccgtcaagaa ttattcggga
    10921 atcagccatt tgaacgtgca ctgattcatc tgatgcgttc ggcttgggat aactatcaat
    10981 tattcatgcc ggaaaaattt tatcttctaa atgaggaaca ggcaaattta tacaaagacg
    11041 tccaaacact tttttccagt tgggccaaac ggctacctta tgatctacgg tttaatccta
    11101 attgcatgcg agcttttgtc atcgaattga gcggaattct ccgtctgaca aaagaacgtc
    11161 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
    11221 aagctgtaac tactttcgat tttcaagttg caccaacaat ctatgcaagt atatctgata
    11281 taaagaagta tgcacagcaa ccctcgaacc gagtgttatg tgaacgtaca ttatatacac
    11341 cggatgcagt ccaatatgag aatattattc ctatttcaat caatacgatc gaacgagcaa
    11401 tcatttcagc tgtgcaaaat aaataagagg ctgtgataaa catcatgtca cagcctctta
    11461 ttctgaataa acaatattcc aaaagtaact tcttcggaaa taagccgaaa ttttcataaa
    11521 tttcttctta ttccttgaat ctgcccactt cttaacaacc tctcgataaa cggcgggctc
    11581 atagcctcac ttatttggga cgtctactgg cccgatttct ttatttaaaa aataagaaat
    11641 gatcgttcgg ataactacca atgcagctag ctggaaaata tcctgcaaag taggtttgat
    11701 aatcgattcg atgatcccag ccgaaacaag aatttctaaa cttaggagta tataacctcc
    11761 taacatcttt ttaatagcat tgttctcgct gactcggtcg tttttcgctt ctgtatggac
    11821 aaataagtat ttcaagaaat ccttaatcga cagacaaacg ccccaaatca ataaacagat
    11881 agcaaaaata tcaagtccca gtacaagttt ttcaaaatat ggaagaagaa actgcatcaa
    11941 ctctgtcgtc atcctatctc tcctcttctt tgtttctctt tgtcttctat ttcctgattc
    12001 aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa aatatctgtc
    12061 caagtcggct tgataactga ctcgatgata tcagcaacga ctagcacttc caagcttaac
    12121 aaaatataac ttgctaacag ttttcttgta tctgcattac gagtattacg ttctgcatag
    12181 tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat cccagcaact
    12241 aatataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa ttcaatcaaa
    12301 tttgagtgaa acatataagc tacctacttt cttttgattt gttcctattc ttttattgta
    12361 aaagaaagag accttgttcg tctatttaaa agttgtttta ttgatcacac cttgcctaaa
    12421 atcctcctct tccctttctc attcttcacc ctccatacca tccatacatc atggaagtat
    12481 ccaaaacaac atagcaagtt aaaataaggc ttagtccgtc atcagctttt caaagtggca
    12541 ctgtctcgcg ctttttttac ccaaatttct aaaaatagaa ccatacatct cctcaaaact
    12601 tttcttcttt cctaccctat tactaattac aggaaaatca gacacttctt cttttttgtc
    12661 aaaaattcct ttttcttctc accctatcat ttggcattct cataaaaacg aactatcctt
    12721 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
    12781 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaaa cagggctatt
    12841 cgaagatgac agtcatttat atgatgaatt tgaatccatc tagcatctta tttcgttgaa
    12901 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
    12961 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
    13021 tggaaagggg accagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
    13081 ccgaccgaca ctgtagtgga tgaatattgg aataaaaatc atccattaga aaaccaaaaa
    13141 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
    13201 ctattaagtc gaataccttc tgttactgag ccaggaatga ctgctgaaga agatacgaga
    13261 gattttgatg caaaacaccg tacctttgac aaagcacgtt tgatggaagg tggtaaaggg
    13321 atcattgacg gcggaaaact gggcttcaac aacaaggacc gtattcttct gactaaactg
    13381 attgccatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
    13441 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
    13501 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
    13561 ttcactcaaa tcgaacattt agtgggcgtc aatcgaacac gttacaatca gtacgaaagt
    13621 atcatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
    13681 cgtgtcactg actgggaatt caaagataca ccgatgcaag atgaaatcac tgtcactggt
    13741 ttgaaaatga ctaatacatt gactcaggaa gaggaacatg tcacggtaga tgaagataca
    13801 gcggtcattt tcacgaatgg ttccattaca gattcagcta ctttgggaga ttacgaaaca
    13861 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagcgaa
    13921 cgtttctata acttaggaaa tcccgacaaa tttttcgcgg atcgagatgc gagcgaatgg
    13981 gtcagcttta cactgacgac gaaaaaccac ctgttattga atgaaatcac ccgtatcacg
    14041 actcaagaac caggaaatgc cttgaattca tttatttcaa ctacaccgat cacgccatta
    14101 ggccaaaaag atgtcaatat gtctatcgtc gtgcatcatc aaccgcattt tacgtctcaa
    14161 aaaccgaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
    14221 gtggataaac agtatattaa gatgaacgga aaagaaatgt tggaagaact gatcggacaa
    14281 ctttcaaaag tagatcctgg tccagtgaat attcgggaaa aagaaacaga gattttcgac
    14341 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcattatt taataaccgt
    14401 gcaaaaagtg atcgaccaaa agtcattcct aagcattcaa ccaatctggc ctttacagga
    14461 gaatttgttg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
    14521 gaaatcgctg ctttccattt tgcgggcatc cctatgtccc gtcttgtcaa aacaccgcgt
    14581 tacgacaaag acatccctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
    14641 ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagcaaaa acagctactt
    14701 atgtttttga cttggttgga cttattatcg cagatgcaag cttttgaaca ccgtttatca
    14761 agaggttgtg aaaaagacgt ttaatttcga gtattaagaa aaattttgtg aaaatggttc
    14821 ctcacatttt tgcataattt ggcttaatac cgagaaactg gcttttgaac accgtttacc
    14881 aagaggttgt gaacctaccg tttagctctg agtcacaaga agcaatttca aaaaatagct
    14941 tcctatgttt tatgaatatt gtgaccgagg agttggtagg ggaaccccat ttattcataa
    15001 caagaggttg tgacagattt catgtcacaa cctcttatta tttatatccg tttctttttg
    15061 aaaaaacctt tttttcagta ttcactctct tgccaatcca ccataatcca gctgctatca
    15121 ctacttcaac gattgctact acactaaaaa attggatgaa tgacaaaata tatgtgtcta
    15181 acaaatgttc gagtgcttgg gttcgaatat ctaactgact ggccatatcg tacaaataga
    15241 gactgactga cagacttaat aattggaagc ccgcacctcc tatcaccact gcaaatgacc
    15301 aaagaacttt tttccaagac attctgtata aataaaacac tccgctcagc aatacaagca
    15361 agatactact gatcattgga agaaaagaaa ccattcgctc tcgtatcgtc aatatctgat
    15421 cgatgaacga catcaaatag attgattgta ttcctcgtcg aaaaatccca gaagtcatat
    15481 agatgagtaa attgttttct tcactagcaa atggatcatc ctctgcttct tcttgtttgt
    15541 atgtattgat tatctcttgg agcttttctt ctacttcctt ttctccaatc agttctactg
    15601 gttctttttc atatctgttg cggatgaaaa gctcgatatt tttttgtact agttcttcag
    15661 aaacaatctt ttccaatgct tcagccggag ctccgatctc ataatgatag ctttggatct
    15721 ctcgattgat ctcagcagct acttttttcg catagtccga ttgcatgttt tgctctaaca
    15781 taaaatgttg atttgccaag gtgcttatta aacaaagcat aacagtcagg caaaacaggc
    15841 agacagaact aatcataaca atgatccgtt tattccattt attttgaatt tgtttcatta
    15901 cgcctcctcg tatttgtttt ttcactgaaa cgaatgatgg ttggttattg tttaaatagt
    15961 taaaatgatt caatagttca actatacttt actgtacgta aggatacaat gaaggcacac
    16021 tccttctttt tctaataaaa aaaaattttg gaactcttgc ttttttgagg atttttattt
    16081 tgagaaaaaa gcgtttcatt atcaaaaaga taagaactcc tttagaatga aactatctgt
    16141 agtaccaata atgcagacaa ttttgtgaat ctgaaaggag catctccaat gaaagcagtc
    16201 acttggcaag gaaaacaaaa aatggaaatc agaaacgtag atgatccaag aatattagaa
    16261 ccaacagacg caatcatccg tatcacagca accgccattt gcggctccga tctgcatctt
    16321 taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggtcacga acctatggga
    16381 atcgttgaag aagtaggatc acaagttcaa acattgaaaa aagggcaacg agtggttatc
    16441 ccttttaata tcagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt
    16501 gatgaaacaa atccagatca gttatatggc gggcttttcg gttttggcaa gttgaatggc
    16561 aatcattggg gtgggcaagc agaataccta agagtacctt atgcagactc tacgtcgttt
    16621 gtcgttccag ataataatct tcctgatgaa aaagttttgt ttttatcaga tattttgcct
    16681 acagcttatt ggagcgttga aaatgcaggc gtaaaaaaag gcgatacagt tgtgattcta
    16741 ggatctggtc cagtcggtct atttgctcaa aaattcgctg taatggctgg agcagagcgc
    16801 gtgattgcag tggatccagt acaacatcgt ttagataaag cggctaatta caacggcgtg
    16861 gaaacttatt tcttagaaga cacagcacaa gctggaacgg aattgtatga gttggctaaa
    16921 ggtggtgccg atgtcatcat cgattgtgta ggaatggatg ggttagaacc tgtaaaagaa
    16981 aaagcaaaaa atttggtgag cctacaaagc ggcacgattt ctccattaca gatggcttcg
    17041 caagctgtca aaaaattcgg aaccgtccaa gtaactggtg tgtatatgac gccagcttct
    17101 tcttacccgc ttcaagagtt tttcatgcga gatattgcgg tcaagcatgg acaggcacca
    17161 attatccatc tgatgccgaa aatctacgaa atgattgcag aaggactatt tgatcctaca
    17221 cagatcatta ctcattcgct gccactagaa gaagcagcac aagcatatga gatctttgac
    17281 aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaaatcagta aaaaatacag
    17341 gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa
    17401 gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaaccg ttacacattg
    17461 atctcactag ctgtcgactt attaaccggg attttctata tttttggaag ccttgcttcc
    17521 ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc tgttttcttg
    17581 acgattcgtc ctattctcag aatcgttagg aacattttca tttacgacga gaaaagaaca
    17641 aggaaaaagc atgggaaggc agtttagcct tctccatgct tttttgttat tcttgtgttg
    17701 tgcatatttc gcatactgcg attcgaaaaa tttctaagta tagaacaatt tttcggtttc
    17761 cttctctata tactcacgtc tatgctgttt catgtatcta ctcttacagc gtcatttggt
    17821 acactcgcac aggaattcct tcattatcct gagccatccc gataaactgc caattgtatt
    17881 tttcataaaa atccgtatga tccgtcacaa ggtaaagcac ctttacgccc atttctgcaa
    17941 agtcttgctt gatcgtttct aaaatcattt tggcaatccg ttggtttcgg tagtcttttt
    18001 caacaaacag cgcacagaca ttcggagtca aatctactcg atcatgaaaa tcattgtcaa
    18061 caacacctgc acctgcaata atctctcccc attcattcaa caccacatac cattgcggaa
    18121 ccgatgtgtt ttttctcgtg cttgcttgga tactttcttc ataaacagca actggcagat
    18181 gccattttga agcaaaccat tcggctgttt cttttatcaa tgtcggtcga tcccgcaatt
    18241 ttatgactgt tcttctctcc atttgtactc tctcccattc atatttggtc tattatagca
    18301 ccttgtattt caaatagctt tccaatcgct cgattttttt gaatgaatgg gattttttat
    18361 ttatacacaa atttcaagac aaatatacct cgattttctc attcattatt cgatcctaag
    18421 ttttaaatta cttatttcca taaaaaaaca gaaccagata accggttctg ctttcttttt
    18481 tcattaagca tccaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg
    18541 cagatagatc aaaatagaaa ggatcagatt gacagcggcc agtacaccac ctgcaatact
    18601 cttcgttgcc aatacaccgc caataactgg tggtgttacc catggtggca taacagtagc
    18661 tgctggcact aaacctgtag aagtcgcaaa gtacgcaaca ctaactaaga ccattggtgt
    18721 caaaataaat ggaatgaaca tgattggatt caatacgatc ggtaaaccaa acatcgttgg
    18781 ttcattgata ttgaaaacac ctggcgcaat cgacaagttt gttacaacca tatacggttt
    18841 gtttttacgt cctactaaat aaatagctaa cagtaaacct aatgttgcac ctgttcctcc
    18901 taaattgaca aagctgtcga agaaaggttt gttcacgata aatggtgcgc tatgtccagc
    18961 tgcgatcgca ttgacatttg cttcgattgc cggcgcattg atcgtttgca tcaaaggatc
    19021 gaccatattc gccccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcaa
    19081 taatgcagat ggataagagt tcgcaagtcc cataaatggt tcttggacca ctgtatagaa
    19141 cgaacctacg atatcatcaa ccccaaatgc agcaaaaatt gcagcaacta aagcaaaggc
    19201 agagatagtg atcattgctg gcagtaatga agcaaatgct ttcgcaactg ctggtggcac
    19261 accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt
    19321 tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc
    19381 gcctaaagca aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta
    19441 gccgttagct aaatgatacg caagtaagaa agcaatcaag acagaaagaa tggcaaatgt
    19501 gccgttccaa gctgcagccc cgaatccttt ccatgattca ccgccaaaaa ttgaattcat
    19561 caattcttgg aatccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaaaat
    19621 cattaacggc atactcacca taaatgaatc ccgaatcgca actaaatgcc gttgtgcacc
    19681 aatttttgat gcgacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa
    19741 tcttccctct tttctttact gtgaattacg cgtccaaccg acgatacaga tcaatgatct
    19801 cttttgctaa atctgtaaaa gcaatagatg tcatcaggtg atcttgactg tgaactgtca
    19861 aaagtgttac ttcaacgtgg tttccttgtg cttcttgcgt cagcatctct gtttgagagt
    19921 gatgtgcttg gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat
    19981 ctcctttttt cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca
    20041 tgatcagacc catgatggct tctagatttt tttgatcttc catttcttgt ttcctccgat
    20101 atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca
    20161 tgccatagtc agccatattg atcacatcta gaggaatgcc tttaggtgct agcttttcag
    20221 caaattgatt ttgcataaaa cgtacttgcg gacctaagag taaaacgtct acatttttag
    20281 attctatttg attatctgca tctgaggcag agacagcaaa gatatctgct tccattcctt
    20341 ttgcttctgc tgctttttgc atttttgtga ctaggaggct tgtactcatt cccgctgaac
    20401 atacaagcat gattgttctc ttttccattt taaacacgtt cctttctatt cttacacttg
    20461 tatataaagc aagtagtgtg ccaactttaa aagcgcttta aaaagttgat aatatcacgt
    20521 ttatggtcta taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa
    20581 ttctttttta ttgtatttat ttcttttaac gtgcacaaaa aaagttgtga caagtctttt
    20641 gtcacaactt ttttgaataa gaggtcaaaa tgctactttc acaacctcct ttattccaaa
    20701 taaccatcaa atacaatcaa tatcttcgaa aaacttctag ttatttatag gcttcatcag
    20761 gtaagcttaa ttctctctta tcaatcagag actgatacca ggaagcagac tttttcaata
    20821 ttctgctccg ttcatcgttc aattcgattc gaactaatcc atatcgattc ttaaatgcat
    20881 tcattggtga aacacagtca gtgaatgccc atagcatata accttcgcaa tttgcacctt
    20941 cttctgctgc tttgatcaaa taacgcaaat gctgtccgat aaattcgata cgatagtcat
    21001 cttgcacgat tcctttctca tccatgtact gttcttcatg ttctcttccc ataccatttt
    21061 ctgttatcag ccaagggata ttatgataat tttcttttag atacatggct atatcgtaca
    21121 tgatttttgg atagatctcc caccctctag aattgttcat tttctttcca ggtaaatcaa
    21181 agacttcata atatcgttct ggatggaaag gtgtctcttc attccattga taacgaggag
    21241 cacgaacgcg tttagggtaa tattgattga tcccaaggaa atctactgta tgatgctgaa
    21301 ccgtttctaa atcctctgtt gcaggatcga aataaatcga ttgttttcga cacaactcta
    21361 ggaactcttc tgtgtagtac ccttttacca tcggatcaaa aaatactcga ttataaaaca
    21421 gatcatacat ataagcagct ttttggtctt ctggagaact ggaacgagca tagaccatct
    21481 caggatttaa aatacagcca atccttccgc cataattgcc actatggtaa atttctacag
    21541 ctttagccgt tgctaatact ttgtgataat tccacatcat ccattttttt gtgttttgtt
    21601 catgcggcca gcgaacagca tccaaatagc aacgcgtttg gggaacgatt ggttcgttga
    21661 atgtaaacca gttttttacc ctgtcaccgt aacgatcaaa agcaatcttt gcgtacttag
    21721 cataaaaatc aaccactttt cttgaagacc agccatcata tttttccaaa agaaaagcag
    21781 ggacttcgta gtgttctaaa caaatcatcg gttctacgtc tgcttcgatc aaagagtcga
    21841 tcatttcact tatatgctga gcatattctt catctactat acaattctcg taatcagtga
    21901 aaaaacgagc ccaattgata gaagtccgat aatgtgtcag atggatggat ttcatcaaat
    21961 ctacatcctg ttggtactgc tccataaaat tagttgctac ggctggacca tacccattat
    22021 gccaaacaaa atgttcattt ttataccagc tatctataaa cgaatcctgt ccttcttttt
    22081 ttcctttcca accttccgtt tgccaagcag aagatgctgc accaagaata aaattttctg
    22141 gaatagtaat tttcatgatt tcctcctgtt ttttagatca catcattttc cacacgattt
    22201 gctactttta cgaatggcag atagatcaaa atagaaacaa caatacaaac aagttgcgta
    22261 ataactgcac cgatatttcc tgctgtggcc agataagcat tgacgatcgg cggcatcggc
    22321 caaggaacca ttacaactgc ttttgccgca aatcctaaag cggttgcaaa ataaccaatc
    22381 gttcctgtaa caagcggcgt taagataaac gggatagcta aaataggatt cagcatgatc
    22441 ggcataccaa aaataactgg ttcattgatg ttgaagatcc caggagcaag agataacttg
    22501 gtgatttgtt tcatatcttc tcgctttccc actagcaata ctgctatcag tagacctatc
    22561 gtgaccccag acccgcccat actcatatac atatcccaaa atggcatagt gataatgttt
    22621 ggtacttctt ttccagcttc aaaagcttcc gtatttacag caattgccgc taaaagcaac
    22681 ggttctcgaa ttggcttcac catttgattt ccatggatac caatcaccca aaagacttgc
    22741 gcaatgaaca tcaataacaa aatacctggt agcccttgaa cgacaccttc taatgggcgt
    22801 tgtacgacat tgtaaatgat atcataaagg tacatccctg taagttcttt gatgataaac
    22861 cccgcagccg cgatacctga tactgtcaga atcgtgggaa ataacgcaga aaaactggca
    22921 gaaacattcg ttggcacggt atcaggcatt ctcagctgta gttttttctg tttcctcaac
    22981 cagcaataaa gttcaatgga aaggatggag accaacattc ccaaaaataa ccctttcgta
    23041 tctgtatatt gtttagccaa tacgttctct actataacag tctgattttc attcactaat
    23101 tcataaacag ttggattaac agataaataa gacatcactg cgataatacc agggaataca
    23161 ccatccactt tattcagctt tgctaattcg atccctatca gaaaaacagc atagatggtc
    23221 aagaaactta gtgtgacata gctgatggca gatgcgatag gttttaattc ttctaaaaaa
    23281 cgcaaaaaac ttatctgtgc taacccattt tccgaatcaa agaccattgc tgaaaacaac
    23341 gtgccaaaag cacccgtaat aattaccgga atcaatgcag caaatgcgtt ttttatcacc
    23401 atgatatacc ttaaattatt gattctcgtt gccacgttcc ctaaaacatt ggctaattta
    23461 tccataaaac tcattccaga tcccctcatt cctgcttctt ctccttgccg tcatctcttt
    23521 ttgcctgtta gtcagcgctt acatttcggt gaataaaaac tatttgcttg tccgttttac
    23581 caaatataaa acaaaaattc atctgatgta tttatcataa aatacatcag atgaactgtc
    23641 aattagtttc tttcttccca attattttct gtaattctct ccgatttgtt gccatatgga
    23701 cagtcatcgc atcaaatgcc gcaagtacat tccgcctctc gatagcctga acgatttctg
    23761 tgtggatatc gatcgtctcg attttcaaat gctggatatc ataatttttg ttaaataaag
    23821 caatcgattc attaatgatc ggaatgatat gcagtaacgc tacgtttccg ctagcttctg
    23881 caatcgtcgt atgaaaatcg atgtctaatt gaacatgatc ttgtgtacct tctcgaaaag
    23941 acgtttcaat cttttgcgtt aagcttttca ttcggctgat ttgatttttc gttgcatatt
    24001 ccgccgctaa ccctgccatc tttggctcca gtaaatatct cagctcaaac aagtctcgta
    24061 tcatccggtc ctgatcttca atcaaagaaa aaccgagtgg atcattagca atccctcgct
    24121 tatcactgac aaatgtcccc gccccttgcc gaacttcaag aatattccta gaagcaagcg
    24181 ctcggacagc ttctcgcacc gtgctgcgtc ccacctctaa ataatcggac aactcatatt
    24241 cattcggcaa tttatctcct acttgcaact gcttttcctt taaatatcta aggatcttgt
    24301 ccgtggttac ttctactagg gttttatttt cttccaaacc acatacctcc ttttcatctg
    24361 atgtattttt tcgtaattat tataacacgt ttgaatcatc tgatgatttt ttgtataaaa
    24421 atcgattcat ccgttcatgg atatacaaac gaagctattt accataaaaa aagactagga
    24481 taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaacgt atgatgttca
    24541 ataatagatg agcttcattc gattcttgat gacatttttt gatagatgga taaaaagttt
    24601 gattacatcg tttctattct agaatttggt cgatcttcaa ttcttcttct ggtgagaaat
    24661 ctaaattatc catcattcga acactttctt gtaactgact taaccgactt gctccaacca
    24721 gtacactcgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc gctaatgatt
    24781 gccctcgtga tacggcgatt gtctgcagtg ctttgatttt ttccagagtt gatccaactt
    24841 gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca ggaatcccat
    24901 gcagataacg atttgtcagc aaaccttggg caagcgggct gaacgtgatt gctcctagac
    24961 cttcttcttc taatactgga gataagtcgt cttctagtag ttcacgagaa aacatattgt
    25021 agcgcatctg atgaataatg aacggtgctt cttttctttt taaaatttca gtcatttttt
    25081 tggtatcttc tccattatag ttggatatac caatatataa tgctttccct tgtcttacaa
    25141 gttgcatcaa tgcttctgct gtttcttcca aaggtgtatc agggtctgga cgatgatgat
    25201 agaagatgtc gacataatct agccccattc tttggagact ctgatcacaa cttgaaatga
    25261 tagatttacg agagccccat tctccgtatg gaccttccca catatgataa cctgctttgc
    25321 tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata cgcccgaagt
    25381 ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa tgggtaattc
    25441 ccatgtcgaa tgcacctctt aaaatctctc tttggttatg gataggatca taatcaccaa
    25501 aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga ccggatttac
    25561 gatattccat tttttcataa cgtgcactat ctgcaatata caagttttct cctccttacc
    25621 atttttaaaa cgctttcttt cgttctgctt ttagtttact acattttaaa gaaaaggagt
    25681 tagtcaaaat gattttttct tgtatttttt taccatttgt tttttattcg ttgatagatc
    25741 agaaattgtt tacgtgcttg ctgaattttt ttatagcgtt tcggttgttt tttgtaaaat
    25801 tgctgatgat aattttctgc tggccaaaac gtaccaactg gttcaatttt tgtgacgatt
    25861 ggttggcgga acgttcctga actgactagt tcctgtttcg attgctccgc taattttttc
    25921 tgacgttcgt cttgataaaa aatcaccgga cggtactgct tccctctatc ttgaaattgt
    25981 ccaaaggcat ctgtgggatc agttatttgc caatagattg tcaacaaatc gctataactt
    26041 atctctctcg tatcataaat gatctccaca gcttccacat ggcctgtgta accaccactt
    26101 acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa aacggatagg
    26161 ttcccttttt tcgattcaaa cggctcgacc atgcaccaga aacatccccc tgcaaatact
    26221 gcacgttctt gatagtcagc atcttgcata gcatgcttgg aaaagtcata tttttgttgc
    26281 gtgtcaaatc cttccgtaag caataaataa aagtccatta cgtcaggcgt cagattcatt
    26341 cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt agcggatgta
    26401 ttctctctta tgtcattctt tgtctggatc aatacttttc gttcccaatc tcgtgtattc
    26461 ggatttaaaa tcagattata aagtgtttga atgaatactt gttccttatc catagactct
    26521 cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata aagtgaggaa
    26581 ataagcgatt gaatcgtatc tattcaaaga agataagcgg cttcagatga tataagatga
    26641 tctttatctt aaaaagtata ctctatggtt tggtttaact aaaaaacgac ctctgaaaaa
    26701 agggatgttc tccagttctc agaagtcgag taaagatgat tcttttttta tgaacgataa
    26761 taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg ctgccgcact
    26821 ttgctttcga atatttcatc gattgtttct atcggcatgg cacctgtacc gatttctagt
    26881 aatgttccca tgatgattcg caccatttta tatagaaatc cgtctccgac aaaagtaaaa
    26941 tgaagcattt ctcccttacg ttcgatagat aattcatcaa tcgtacgtac cgtagatttt
    27001 tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccatcaa tttttcgcaa
    27061 gctgcattca gcttatccat atccaactct tgaggaaaat aaaagctgtg atttcgttca
    27121 aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc caccgcatta
    27181 tatcttgcat ggaagcgctc aggcatctct tcgattcttt tgatgacaat atctcgcgga
    27241 agatagcgat tcataaagtc gatcatttct gccagtccaa tttctgagtt tgttttgaaa
    27301 tttgccactt gtcctcgcgc atgcgtgccg gcgtctgttc ttcctgatcc aataatttct
    27361 atctcttcat ttgtcatctg atgcaacact ttttcgattt tcccttggat ggttttatcg
    27421 tcatctccaa gtctttgcca gcctgaatat ctttttccgt catattcgat cgtcaacttg
    27481 atgtttctca tatttttgtg gctcctctat attccatttg gtcttctctc taccttttta
    27541 agattgtagc acaaattaaa gctacttgct tctctgtgag agaaaccaag tagctttaaa
    27601 taattgttta tgaacaattg tattatttat aattcataat gaatcacttc tttatattcc
    27661 tcatataatt ctgcgtttcg cagatcgccc ccatctaaat tcgccgaata aaatatagga
    27721 ggatgtttta tcccatttgc aataagctgt tcgcaaactt ccacgatgat cgtattgagg
    27781 atggctgcac cgattacagt agaggaagga ccggcttttt gtacgatacc tgtcagctca
    27841 cagcaagcat ctcctatatc cccatggtta tcgataacta tatcagaaac ctcaaacaat
    27901 cgtttccctg atgaatgacg actagtgacc gatcgtgaat attggacatt cgtcaaacta
    27961 atgattttta cgcccttcgc ttttgcagtt aacgctaaat caataatgat tgggtttctt
    28021 cccgataccg agtgaaggat caatacatct tgatttttaa atgaaactgt ttttgctaaa
    28081 ttcgttccat atccttctaa acgttccatt tgactggtaa aggtgatagg ggaacgatct
    28141 agcatcactt ctcgtccaaa gatagcattg atggtcatca tcccacctgc ccgataatac
    28201 atctcttccg ctaagatacc agcatgacta gcaccaaaaa tatagaccga acgtttttgc
    28261 agattcgctt gggtcaatac atcgattgct tctttgattg actttttttc ttccttttca
    28321 accaaatcta ataactgttt gattttatcc atatatttga acatctagaa ccctccaaac
    28381 aaaccgctaa atcctctacc gatcattccc cataacgaca aatcgtttcc gccgaaaatc
    28441 aatatccagc tttggatcgt cgtactaaaa acaatagctg aaaaaccagc taatacgacc
    28501 atgacaaatg aagctgcgat tgttccgaca accgctccac gaaagccacc ttgaccttca
    28561 ccgatgactg ctgcaccacc acattcaaag aaacttgtga ttactaatgg cacaagcaac
    28621 acgccgaata gatggaatga atttgccaaa acaactaaaa tcgtagaagt gatcattgcc
    28681 acgataaaac cgataatcac tgcatttggt cgataattga ataaaatagg cacatcaaat
    28741 gctggtttcg ctcccggtac gactttttct gcaatccctt gaaacgctgg aatgatttga
    28801 ttgatcagta gtcggacacc atatagcata atgagtagtc ctgcaccaaa attcaaccct
    28861 gcttcaatcg aaaacatcag gagattatcc ccttctggta tcaaaacagg tgcgatgatc
    28921 ccaactacgg tattcatcaa gaacatgact agtgcacctg taattgagat ttctcggaaa
    28981 aatgaaagat tctcaggtac tttgatatct tctgtcgatt tttctttatt tcctactcgt
    29041 tttgctacaa atcctgaaac taaagacaaa acacccgttg gatgaccaat caggaaagaa
    29101 tcatctcccg tagcatccca gacatatttg cgcatgatcc acggcataaa agaccaataa
    29161 caagccgata aaatcccgct gatcccaatc aatgctcccc ctgacagtcc tgcttccaca
    29221 ccagctgcta caaatacgaa aggaaaccac caaagcatat gacctgttaa aaagatcgtc
    29281 ttcacaggtg taaaccgagc aatcaataaa tggataacta atcctaaaaa catagctaat
    29341 ccgaccgttc cgccatattc tgcagtaaac gtcacatcag atagtccttc tgtcacttct
    29401 acacccaact gtgtttggac agctatattg attggagaaa tcgttccgac cagcatattg
    29461 actccggcgg tcaaagcaac cataccaaat gcagcggtca aagctccctt aaccgtttct
    29521 gaaaatgatt ttcgctggat gaacagcccg agcatcgcaa tcaaacctaa caagacgggc
    29581 ggattcttca agatgtgttc actaatgaaa ttcattcttt tattccccct tatcagctag
    29641 atattgttcc aattgcgcct ttatttctga agtatccata tactttttaa taggataaac
    29701 aggcgctttt acagaagctt ttagttctgg aagtaattca tgacttgtaa aataaacatc
    29761 tgccatcgtt gctttggcag tggataaatc tgtattttcc actgtcgcct ttaaacccag
    29821 ctccttaatt actttatcaa gagacatttt caaaaccata gaactcccta caccaaatcc
    29881 acatactgct aaaattttca tttttcttcc tccttatgca aataactgaa tgattgtttc
    29941 tttatttcct gataaaatcg tttgatattt ttcattgtcc ataaatattt ccatgatatc
    30001 ttttaatctc tccagatggc tttcgtggtt tttggcagct aaaaccaaaa aaatatggat
    30061 tggtttccct ttcatatcta cagcatcttt tacgactaaa aggctcatac tttgctcgtt
    30121 tactccaaga cctggttttg cgtgcatcag cgcaaaataa tcagccaata ccatatatgg
    30181 tccattttcc tctacacttc tgatcatgtt ttctaaatac gtatgcgtta tcattttgtt
    30241 ttttaataat ggattgctag cgatttcgac tgctttcatc caattttcag ccgtttctat
    30301 tacttggatt tgatttttcg gcagcaattt attgatagta ctcattctct gacctccaac
    30361 atttttgaaa taaaccaatt tttatcctct attccttgca tgaccatttt catcacaggt
    30421 tcgacttctt tcacatcttc ttctcggatc attagcagaa caacgatatc tgccttttga
    30481 tatccccatg aaaatggctt gtctgctatc aatacagcaa ccgaagtttg tttaacgaca
    30541 ttgggattgc catgaggaat agcaaaacca ttctccaagt atgttgattg gatttcctca
    30601 cgctcaaaaa tagagtagag aaatcgctgt ttcgattctt cattctcaaa ctgttgctga
    30661 tcaaccaaaa aacggatagc agcttctttt gaatcaaagt gatcaaaata gacattagag
    30721 gaaataagag gtttcacttt ttcagctatc gagccttcga tgtttatttg ttcgttatct
    30781 atgaccagat cgatataatg attcatgata cgtttcatat ctctagcagt aaccattggc
    30841 gaaattctta ctactggtct aatttgttta ggtatatcga cagtggagat aatcaaatct
    30901 acgtcaaaca aatctagtct gttcaattta tcgacagatg ctgtttcgat actatcgata
    30961 tcaggtaaga ttcttctgat ttttgcagaa acaaatgaac tggttcccat tccattggga
    31021 catacgaata agagtctatt taatcgtttg acacgctcga ttgctgcttg gaaatggata
    31081 gctacaaaac ctacttcatc ttcggacaag cttacttgat aaggccagcg aaaatcattt
    31141 aacgcaaacc agacaacacc atacaaagaa ggataatttt gttttatctc atctatcaaa
    31201 ggatttacga tggtcgtttg agcctcaata cgtaaaagca gctgatacag atggacaagc
    31261 agatcttctt ctaaacgact atcttgggta agatctatct gaatgcctct actcactttt
    31321 tggatcaatt gccggatttt ttctcgaaat actaaaggga tcgtttcatt tttcatgaaa
    31381 aagcgtaaac cacttcccac aataacataa gtcaaatact gtacttcttc ttgcgaaaat
    31441 tggtaaatac gctgttcctc taactgatgc agcaattcac aaacgagggg atatttgttg
    31501 aattctaaaa aatactttcc atattgattt ttacctttta aactgatttt cttcccttct
    31561 cttgaacgct gaatcatcaa taagattgaa acgatgatac ttgaaaagta gctttctggt
    31621 agctcaattt gcttgtctgc aatgctctcg cgaaaagatc gttcgactat tgctagaagc
    31681 tttcgatcca ctatttctct tacgatctcc aattcatcaa aatgattcag gatcatacgc
    31741 ttcagtacct tctgaacctg tatttcattt ccttcaaaat atgtcccgaa acgatcaaaa
    31801 ctgagattca acccttcttt cgcaaataca tatttgatgt aagaaatatc atttgcgata
    31861 ctgcttctgg aaacataata ttcttcagaa agttgctggt aacttacatg ctcgaaattg
    31921 gacaatctgt tcaggatcgc tactcttcgt agtttgcttt cttgatattg tattacactc
    31981 atttccatca ccttctatat tcactataat tttttcttat aaggagaact agaccaaata
    32041 aaagattaaa ataacctgag aaatcataca ctgctctttc tcagttaata tacgctaaaa
    32101 cagaagctat acaagaaaat tatggaacta tatttcagaa aaacaaaaaa acagccaacg
    32161 gacgaaatgt ccgctgactg ttcaatttga tggctcaagt tttactgtct gagatgcaag
    32221 atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaatcct
    32281 attttattca ggaatgacca agaatttata gaaggaattc ttgccagacc ataaatcgcc
    32341 gcaaaaagca aactgatcac agacaatcca aggtataagt ggcttacgtc catttgccca
    32401 ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact ataacctaaa
    32461 cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata
    32521 ctgaacaaac ctgtaaagat acaatcacct ataccataaa aaccgataga tgctgtaacc
    32581 agggtagcta acacacgaga tgtacttaca aatgcagcat acaatgcagg caatgacagg
    32641 atgaaaaaaa tccctgaaac cactgaccaa ataagaaatg cttttcttac aggactggaa
    32701 atctctccaa atacactaat cacactcttc atttgatcta attcaggata atacaatcct
    32761 aatacgtacg gagtcaaaaa atcactcaac acgcctaata ataaaaagaa gaaaccaaat
    32821 ttacgtaaaa aatccatgat gcgcctccaa taacaattac ctatgctttt aattgttatt
    32881 ataaacgact ttatctcgat ttaacaggat cctctatttt acacgaacat ctacataact
    32941 cattaaaaat ttgaatcgtc ttaaggttca tccctaaata tctaatattc aacgttcatt
    33001 taacccatct aatcgatctt tcaatttgat tttttctcca cttcttttac gcgcgcctaa
    33061 ttctcctatt gcagtgaaca acagatcact tgaggaattt aaggcagttt ccattgaatc
    33121 ttggataaca ccgataataa atccgatacc gacgatttgc atcgcaacat cattagagat
    33181 accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa cacctgaagc
    33241 cccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa atggtgcttc
    33301 aatccctaag gaatgtacag ccgtcaatga catcacggaa atggtaatcg cagcaccgcc
    33361 catattgatc gtagcaccta atgggatcga tatggaataa gactctttcg tcaatttcaa
    33421 tttttcagcc agcatcatgt ttatcgggat atttgcggca gaacttcggg taaagaaagc
    33481 gggaagcgca ctttctttca aacaaaaaag aatcaatgga taggggtttt ctttaataaa
    33541 caaaaacgtg aggaacgggt acacaactaa tgcgacaaac agcatcgtgc caatcaacac
    33601 aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtggcaa tcgattgata
    33661 aaccaatcct aaaatcccta tcggagcgaa tgcaataatc acttgtacga cttgtgataa
    33721 tgctacagat atttgatcca ccaattcttt tgtcgattct gaacgtgctc gaaaagccag
    33781 acctaacaac acagcccaga ataagacacc aagataattc gcatcaatga gcgattgaac
    33841 aggattttgt gtgacatttg tcaacatcgt agataaaaca ctttccaaat tttgcggtgc
    33901 ttgttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac ttataatcac
    33961 tgctacaagt gccgaaagc
//
LOCUS       DANJMA010000026        32521 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000026 DANJMA010000000
VERSION     DANJMA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            132..506
                     /locus_tag="PWU31_001484"
     CDS             132..506
                     /locus_tag="PWU31_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1084037.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="PWU31_001485"
     CDS             complement(620..1249)
                     /locus_tag="PWU31_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1084038.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="PWU31_001486"
     tRNA            complement(1383..1455)
                     /locus_tag="PWU31_001486"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="PWU31_001487"
     tRNA            1669..1742
                     /locus_tag="PWU31_001487"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            complement(1880..2044)
                     /locus_tag="PWU31_001488"
     CDS             complement(1880..2044)
                     /locus_tag="PWU31_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084039.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2339..3667
                     /locus_tag="PWU31_001489"
     CDS             2339..3667
                     /locus_tag="PWU31_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL1084040.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="PWU31_001490"
     CDS             3869..4702
                     /locus_tag="PWU31_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL1084041.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="PWU31_001491"
     CDS             4718..5455
                     /locus_tag="PWU31_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL1084042.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="PWU31_001492"
     CDS             complement(5610..6578)
                     /locus_tag="PWU31_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL1084043.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="PWU31_001493"
     CDS             6810..7643
                     /locus_tag="PWU31_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL1084044.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="PWU31_001494"
     CDS             7677..8516
                     /locus_tag="PWU31_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1084045.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="PWU31_001495"
     CDS             8542..9102
                     /locus_tag="PWU31_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1084046.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="PWU31_001496"
     CDS             9301..11022
                     /locus_tag="PWU31_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL1084047.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="PWU31_001497"
     CDS             11187..12329
                     /locus_tag="PWU31_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1084048.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="PWU31_001498"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="PWU31_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL1084049.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14129..14763
                     /locus_tag="PWU31_001499"
                     /pseudo
     CDS             14129..14763
                     /locus_tag="PWU31_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     misc_binding    14892..15108
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15156..17801
                     /locus_tag="PWU31_001500"
     CDS             15156..17801
                     /locus_tag="PWU31_001500"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL1084050.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18111..19424
                     /locus_tag="PWU31_001501"
     CDS             18111..19424
                     /locus_tag="PWU31_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL1084051.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19414..20067
                     /locus_tag="PWU31_001502"
     CDS             19414..20067
                     /locus_tag="PWU31_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1084052.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20122..20814
                     /gene="radC"
                     /locus_tag="PWU31_001503"
     CDS             20122..20814
                     /gene="radC"
                     /locus_tag="PWU31_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL1084053.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20964..21164
                     /locus_tag="PWU31_001504"
     CDS             20964..21164
                     /locus_tag="PWU31_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1084054.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21418..23097)
                     /locus_tag="PWU31_001505"
     CDS             complement(21418..23097)
                     /locus_tag="PWU31_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1084055.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23099..23311)
                     /locus_tag="PWU31_001506"
     CDS             complement(23099..23311)
                     /locus_tag="PWU31_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL1084056.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23704..25434
                     /gene="efrA"
                     /locus_tag="PWU31_001507"
     CDS             23704..25434
                     /gene="efrA"
                     /locus_tag="PWU31_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL1084057.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25431..27191
                     /gene="efrB"
                     /locus_tag="PWU31_001508"
     CDS             25431..27191
                     /gene="efrB"
                     /locus_tag="PWU31_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL1084058.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27286..27483
                     /locus_tag="PWU31_001509"
     CDS             27286..27483
                     /locus_tag="PWU31_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084059.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27636..28121
                     /locus_tag="PWU31_001510"
     CDS             27636..28121
                     /locus_tag="PWU31_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL1084060.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28225..28818)
                     /gene="clpP"
                     /locus_tag="PWU31_001511"
     CDS             complement(28225..28818)
                     /gene="clpP"
                     /locus_tag="PWU31_001511"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL1084061.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            28998..29519
                     /locus_tag="PWU31_001512"
     CDS             28998..29519
                     /locus_tag="PWU31_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084062.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            29616..30353
                     /locus_tag="PWU31_001513"
     CDS             29616..30353
                     /locus_tag="PWU31_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1084063.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30357..31274
                     /gene="miaA"
                     /locus_tag="PWU31_001514"
     CDS             30357..31274
                     /gene="miaA"
                     /locus_tag="PWU31_001514"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL1084064.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31276..32505
                     /gene="hflX"
                     /locus_tag="PWU31_001515"
     CDS             31276..32505
                     /gene="hflX"
                     /locus_tag="PWU31_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL1084065.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10767 a         5518 c         6874 g         9362 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttgcg
    13921 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
    13981 acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca
    14041 cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata
    14101 tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc
    14161 aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat
    14221 aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg
    14281 cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt
    14341 tcttatcgat gtattaatac taaagaagaa aaacgaatcg cattagtagg atatggaaat
    14401 cttggcaaag cattgaaaca caacaatttt agaagaaatg aaaatctaaa tatcgtgtgc
    14461 gtctttgata atgatccagc actgatcaat cgagtaattg atggagaaat gatctatccg
    14521 attgatcgtt tcgcggagat agccaaagca aaaaatgtga cagtagcaat ctctacggta
    14581 ccaagtaaat attcacaatc tgctattgat gaaattgtca aaggaaatgt aaccgctata
    14641 cttaattttg cgcctgacag agtaactgtc ccagcttatg tcaatgttca atacattgat
    14701 ttgacaaccg agttgcagac gcttatttac tttgatgaga attactcaga agttttttct
    14761 taggaaaatt tgagtaggct tattgatctt gcgagaactc gtcgtaattc tttcaaaaat
    14821 tttttagtac taaatgaagt ggttttttca cgaccacttg ttgactaaaa aaaaaaacca
    14881 ttgtagaatg aagcataacg ctgaacaaga ggagtaactg gcacattcat tcttagagag
    14941 agaactatct gctgaaaggt tctcaatgac aaccagtgaa ggtagcttgg gagtttctgt
    15001 cttgaatgga gtagagatag acgagtcgtg atcgttaact cacttgagag gcaataatga
    15061 ttgattattg caatttaggt ggtaccgcgt aaatcatacg tcctagaata cgaaagtatt
    15121 ttaggacgtt ttttagttta aaggaggaaa acagcatgac ggaagaaaat aacctatcaa
    15181 caaaatataa tccgcaagaa gttgaagctg gacgttatca gcattggtta gaacaagatt
    15241 tgtttaaacc gagcggagat aaaaaagcaa aaccttattc aatcgtaatc ccgcccccaa
    15301 atgttactgg aaaacttcat ttagggcatg cttgggatac gaccttgcaa gatatgctga
    15361 ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct gcctggtatg gaccatgcag
    15421 gaatcgcgac acaagcaaaa gtagaagcga agcttagaga gcaagggatt tctcgttatg
    15481 atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg gaaagaagag tatgcgagcc
    15541 acatccgcga acagtgggct aaaatgggct tatctttaga ttacagtagg gaacgtttta
    15601 cattagatga tggattatct gaagcagtac gtaaagtttt tgttacctta tacgaaaaag
    15661 gtttgattta tcgtggtgaa tatatcatca actgggatcc tcaagcacga acagctctat
    15721 ctgatatcga agttattcat aaagatatcg aaggcgcctt ttatcatatg agctatgaac
    15781 tagcagatgg atcaggcgta gtagaaattg cgacaacccg tccagaaacg atgttgggag
    15841 atacggcgat tgctgttcat ccagaagacg agcgttatca agcgctcatc ggcaaaaaag
    15901 tgattctacc tttagtcaat aaacaaatcc caatcattgc agatgaatat gtagatatgg
    15961 aattcgggac aggtgtagta aaaatcactc cagcacatga tccaaacgac tttgaggtag
    16021 gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga tgatggtacg atgaatgaac
    16081 tagcaggaaa atatgctgga atggaacgtt ttgctgctcg taaagcaatc gtgcaagatc
    16141 tgaaagaaat cggtcgattg attaaaatcg atacgatgat ccacagtgtg ggtcattccg
    16201 agcggacagg tgtcgttgtt gaacctcggc tgtctactca atggtttgta aaaatggcgc
    16261 cgttagcgaa aaaagccatt gataaccaag aaacagatca agcagtggaa ttcttccctc
    16321 caagattcaa tcaaacgttt ttacgttgga tggaaaatgt ccatgattgg gtaatctcac
    16381 gacaattatg gtggggacat caaattcctg catggtatca caaagaaaca ggtgaaatgt
    16441 atgtagggat ggaagcacca gcggatagtg aaaactgggt acaagatcct gatgttttag
    16501 acacttggtt tagttcagct ttgtggcctt tctcaacaat gggctggcca gatacggaaa
    16561 gcgaagatta taagcgatac ttccctacta gcactttagt aaccggttat gacatcattt
    16621 tcttctgggt aagcagaatg attttccaaa gcttggaatt tactggcgaa cgtccatttg
    16681 agaatgtctt gattcatgga ttgattcgtg acgaacaagg gcggaagatg agtaaatcat
    16741 taggtaatgg aatcgatccg atggaagtaa tcgaaaagta tggagcagat gctctccgct
    16801 ggttcttatc gaatggttct gcaccaggac aagatgtccg cttcagctat gaaaaaatgg
    16861 atgcttcttg gaactttatc aacaaaatct ggaatgcttc acgtttcgtt atcatgaata
    16921 tcgaaggaat gaccgtagaa gagatcgatt tatctggaga aaaatcagtg gctgaccggt
    16981 ggatccttac tcgtttgaat gaaacgatcg aacgagtaac tgagctattt gatcgttttg
    17041 aatttggtga agcaggtcga caactgtata actttatctg ggatgatttt tgtgactggt
    17101 acatcgaaat gagtaaagaa gtactttatg gagatgatgc tgtttcaaaa caaaccaccc
    17161 gtagcatctt agtccataca cttgatcaga tcctacgttt attgcatcca atcatgccat
    17221 tcgttacaga agaaatatgg gaacatattc ctcatcaggg aaattctcta gtagtggcag
    17281 aatatcctgt tgtacgtcca gagtttgatg atgaaacagc ttctaaaggg atggaagtat
    17341 tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga agtgaataca ccattgtcta
    17401 aaccaattac gttgctaatt aaaacaaacg atgctaaaat cgatcaattt ttagttgaaa
    17461 atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt gacgatatca agtgagatca
    17521 ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc agaaatcttc ttgccgttag
    17581 caggattgat caatattgaa gaagaaatca aacgattaga aaaagaactg gaaaagtgga
    17641 caagcgaagt aaaacgtgtc caaggaaaac tttctaatga acgttttgtt tctaacgcgc
    17701 cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga ttatttagag aagcaaacgg
    17761 ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata agaatagaaa aagagagtgt
    17821 gaaatcagct agctattact aaaagctagc tgattgaaca tcgttacagg aaagagtttg
    17881 agcctcactc ttagcttcga ttcataagga gatgtgacat aaattttgtc gcatctcttt
    17941 tttagtctac tcttattatg taggagaagg agggaggtgg atcatgtatc aagagtcaat
    18001 aaagtagttg ataaaatcgt tcctctctct gtttcttttc tctttgtcat acttttattt
    18061 ttcttatgtg ttctatttgt tataataaaa gagaaatggg ggaattgttg atgacgattg
    18121 aagaagcaat agaatggatc catagtcgat taccatttgg ttcacgtcct ggccttgacc
    18181 gagtcgaagc tttattagaa gtggtagggc atccagagag aaaagtaaaa acgatccata
    18241 tcgctggaac aaacggaaaa ggttctactg tagcttattt gagaagccta ttagaagagg
    18301 ctgggctgac agtaggtaca tttacttcac cttatatcga aacgttcaat gaacggatcg
    18361 caatcaatgg caaaagcatt ccagacaatg atttgatcca gcttgtaaaa aaatatcagc
    18421 cactgatcaa acaacttgat caatttgaag cagtagctgg tattactgaa ttcgagacat
    18481 taacagcaat ggcatttgat tattttttga tgaagaaagt ggacgtagcg attatcgaag
    18541 ttggcttagg cggcttattg gattctacca atgtagctgt accaatgctg acgggaataa
    18601 caacgattgg acttgatcat accgaaatac ttggagatac aattgaagaa atcgctgcac
    18661 aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac aggaaatatt gccccagaag
    18721 cttttactgt catccaacaa acagctaaag caaaaaatgc accgctttat gtatacgggg
    18781 aaaattataa aataaactat ctgcatcctg atgagatgtg gggagaagta tttgaatttt
    18841 ttaatgaaat aggaaagatc cctcaattaa ccactccgct tttagggcaa catcaagctg
    18901 aaaatgcagg agtagccatc gaattgtatt ttgtttactg tcaattggaa aagctgcctt
    18961 tccgagaaaa agatgttttg aagggattga agaaatctcg atggccagga agaatggagc
    19021 gtttgagtaa cgagccatta gttgttttag atggtgccca taatacgcat gcaatgaacc
    19081 ggcttgttga gaatttgaaa aaagaattca aaggatatca aatcaacatt ttgttttccg
    19141 ccttacgaac taaaaatgtt agagaaatgc tagaacgatt gcagcaagta ccaaatgtcc
    19201 atatttattt gacgaccttt gaacatccaa aagccatcga attaagtgaa gtatcagatt
    19261 tagcaagtga gagagtatca atcgtttcat tgtggcaatt cggattagca gatatattgg
    19321 aaaagatgac aaatgaagac gtattagttg tgaccggttc actttatttc atttcagaag
    19381 ttcgccattt attaatacaa ttaggaggag atcatgaagt ataatgcagt gatttttgac
    19441 atggatggtt tgctgttcga cacagagtta gtgtattacg aagccacaca agtagtagca
    19501 gacaagatgg gttttcctta tgataaagac ctgtatttaa aattcttagg tgtttcagat
    19561 gaagaagtat gggcgaatta tcatatgatt ttctcggaat ttggcaaaac aagtgtacaa
    19621 agattcatag atgattcata ccaggaaacg cttgaacgtt tttcgcaagg agcggtacaa
    19681 atgaagcccg gtgtgttgga actcctctct tttttagaag aacatcagat tcccaaagtt
    19741 gtcgcttcca gtaaccaacg agcagtcatt gaacttttat tgaagaagaa caatctgttt
    19801 gaacgatttg agacgattgt ttctgcagaa aatgttcaac gagcaaaacc agacccagaa
    19861 attttcttgt tagctaatca gaagctaggt acagcgaaga aagaaacatt gattttagaa
    19921 gattcacaaa atggtatttt agcagcttct gcggctgaga tcccggtagt catggtacca
    19981 gatctgcttc caccatccca agaattgatc aaaaaaacag ctggggttgt ttcttcttta
    20041 catgaaatcc ctgcattgtt aaaataaaaa tccatctctt ttcgtatctt attttagata
    20101 agatagaaag gagatgtttt tttgggaaaa cgattgatca aagaagtacc tactagctct
    20161 ttaccaaggg aacgtatgga aatttatggc gcggaagctt tatcggatca ggagcttttg
    20221 gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga tgtcgattgc tggaaatctg
    20281 ttaaaaacgt ttggaggact ggcctcttta cgacaggcta ctttgcatga gttagaagag
    20341 attcgaggga taggacgcgt gaaagcaatc gaaatcaaag ccttgatcga attaggtcga
    20401 cgaattcaga cggatcatga aagagcggct ccaccggttc gtaccagcta cgaattggcc
    20461 cagcaattga ttctggaaat gaaagatcac aaacaagaac atttggtttg tatttatttg
    20521 gacacgaaga atcaagtgat cttaaaaaaa acagtgttca tcggttcgct gaatcagtca
    20581 gtcgctcatc ctagagaaat tttccattat gctgtacgtt attgtgcagc aaggatcgtt
    20641 ttagcgcata atcatccttc tggtaatgtt attccatcgc agcaggatat gaattttacc
    20701 aaaaggatac aaaagtgtgg agaaatgatg gggatcactg tactggacca cttgatcatc
    20761 ggacgcaaac gatattttag tttgagagaa gaaggaatga tggaagagaa gtaagttgct
    20821 ttcttgcaaa aaaaatgaag caagtgtaca attatattgt aattttgttt gggaatagaa
    20881 gttgaaacat ctgttgcacc tctatcgtac acgagcaatg ttacaattta aattagtgct
    20941 tttggcacga ggaggatatt attatggaaa acggaacagt aaaatggttc aacgctgaaa
    21001 aaggatttgg atttatctca cgtgaagatg gaagcgacgt attcgttcac ttctcagcta
    21061 tccaaggtga cggattcaaa acattagaag aaggtcaagc agtgactttc gacgttgaag
    21121 aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc ttaattttat ctgtaaatca
    21181 aattgatggt agacatcaat ttttacgaga gaacaaagcg agtaacaaaa atacctgcca
    21241 caagagacaa tttctcttgt ggcaggtatt tttttgtatg aaaaagtaaa taaatttaag
    21301 aggctatgaa agagccacct tgtatcaagg aataagaaca aacaaaaatg gcttctcatg
    21361 tttttcattt gttttctctt atattcagga caaggtgctg atttcataac ctcttattta
    21421 ttcttcatct ggtgtcatta ccattggcaa aatcattgga tgtctttctg tacgttcgaa
    21481 taagaaaggc tgaagcgcgc cagtcatcgc ttctttcaat ttatattctg aacagtcagg
    21541 tccattcaaa cattctctta aagcgtggaa taagattcgc tgagcttcat tgatcatttc
    21601 tcctgattct cgcatgtaga caaatccacg agagagaata tctggaccag ccaagatttc
    21661 ttttttcttg atatccactg tcgcgacagc taatacaaga ccttcttctg aaagcaggtg
    21721 acggtcacgt aaaacgacat taccaatatc accgatcccg ctaccatcga tatacacatc
    21781 tccagccaca aaatgcccag caggacgcgc agaatcgcta gttaacgcta aaacatcgcc
    21841 attttccatg atgaagcagt tttctgcagg aacacctaca tcttgtgcta gagaagcatg
    21901 tactttcaac atgcggaatt ctccgtggac aggaataaaa tattttggct tcatcaaacg
    21961 aagcattaat ttttgttctt cttgtccacc gtgaccggaa gtatggatgt tattgatttt
    22021 tccgtgaatc acttctgcac cagcttcaga cagcaaattg atcaaatgat tgacacttgt
    22081 tgtattacca ggaataggtg aacttgaaaa gatcactgta tcgtctggct ggatttggat
    22141 ttgacggtga gtaccattcg cgatgcggct caacgcggcc atcgcttctc cttgagaacc
    22201 tgtacatagg atcatcgttt cattggcagg caagcgattg atttcgtgtg catctacaaa
    22261 tgtgccgtct ggaactttga tatatcctaa acgttgaccg ttgacgatgg cattttccat
    22321 gcttcgtccg aaaacagcga ttttacgacc agttgccaca gcggcatctg ctgcttgctg
    22381 caaacggaag atgttagaag caaagctggc aaaaatgata cgtccttcga tttttgaaaa
    22441 gatcttcaaa atagaatgcc caatcgtttt ttctgattta gtaaatgttg gaatctcagc
    22501 atttgtacta tcagataaaa ggcacaagac tccttcttcc ccgatacgag ccattttatg
    22561 caagtttgct ggttcgccaa caggagtgaa atcgaatttg aagtctccag tcgctacgat
    22621 atttccagaa ggtgttttca ccacgatccc taatgcatct gggatactat gggtcgttct
    22681 gaaaaagctg acagaagtct ttctaaaacg gatgatcgta tcttcattga tctcatggag
    22741 ctctgcatct cgaagcaagc catgctcatc taatttgttt gtgatcaacg ctaatgcgag
    22801 aggaccagca tagataggaa tatttgcttg tttcagcaag tagggtattc cgccgatgtg
    22861 gtcttcgtgc ccgtgagtaa tcaccaaggc ttttactttg tgtaagtttt gtatgatgta
    22921 gctgtagtcg ggaatgacat agtcaattcc aagtaaatcg tcttccggga atttgatccc
    22981 ggcatcaatc aaaatgattt catcttggaa ttgtacccca tacgtatttt tcccgatttc
    23041 acctaatcca ccaatagcaa agacgcctgt ctcgttattt ttgatggaaa gtttcatatt
    23101 aaaactccac taatgagaaa tcagcatttt ctttttcgta ctctagatga cttccttcta
    23161 gaagttgaac gaattcaata ttgtatgggg tattttcttc cacacgttgg cgagcgtcta
    23221 catcgctttt tgcctcaatg taaagagatt ttgtttcttc ccgtttaggg ttgcgtactt
    23281 ttgtttcttg ataataaact ttatagatca ttgttttgtc tcctttatct gcgtaaagca
    23341 gtgatttagc tagttgattt ttttagacaa cacaaaacga ggggacaagc cacctggttt
    23401 ttcatatgct ttgactagaa tactggattc gagtcaaaga gacaagcctc gtttttgctt
    23461 atctgttcgg atattttttc aaccgtacct tctgtataaa aaatatacaa caattaattg
    23521 ttattctagc ataaaacagc aaataagtat agaaatcaac tagattgaaa gaaaaaatca
    23581 atgtttatca tcccgctata atcataatga cttttttatg agagaagtgt tctattaata
    23641 ggctcgttgt gctatcattt ttagtaatga aaatgtaaca aaagattaaa aggagatgag
    23701 aaagtgaaac taatgtggca ttataccatg cgttataaga aattcttgtt tttcaatttc
    23761 atatgcgtat ttggttttat tttgattgaa ttaggacttc ctacgatttt agcacgtatg
    23821 atcgatgtag gaatccgcaa tgatgatttt gattatgtga agcaacaagg gatatttatg
    23881 atcgttatta ctgtaatcgg tattattttg aatatcttgt taggttattt tggttcgagg
    23941 attacgacta atatcgtagc agatattcga gatgatttgt tcgaaaagat ccaaagttat
    24001 tcgcatcagg aatacgaaaa tctaggcgta tcttcattga tcactcgtac aacaaacgat
    24061 gcttatcaga tcatgttgtt cctgcaaaat attttacgta tcggttttat ggcaccaatg
    24121 atgttcattg tcagtttgta catggtcatg cgtacaagtg tgacactcag tttgtatgta
    24181 gtaggtgcat tacctttact attattggca gtcgtagcga ttgctaaggt gtctgaaccg
    24241 ttatctaaaa agcaacaaaa aaacttagat aaaatcaaca gtatcttgcg ggaaaatctt
    24301 tcaggattgc gtgtgattcg tgcattcgtc aatgaaaagt ttgaagaaaa acgttttgct
    24361 gtagtcaatg agaattatgc ttctagttca aaaagcttat tccgtttgat ggctgttgca
    24421 caaccgggtt tttatttctt atttaatatc gtaatggtat tgatcatctg gagcggggca
    24481 gtgcaaatcg atgcaggcga gcttcgtgta ggagatttga ttgcgtttat cgagtatata
    24541 ttccatgcct tgttctcatt catgttattt gctagtgtgt tcatgatgta tccaagagca
    24601 gctgtttcag ctggacgtat ccaagaagcc ctagatgcca aaccttcgat tgtagaagat
    24661 ccaaatggta tatctgatac aaaaactaaa gggtatatcg aatttcaaaa tgtaacattt
    24721 gcttatccag gccattcgca agagccagtg atacgaaatg tcagttttac agcctctcca
    24781 ggagagaccg ttgcttttat tggaagtacc ggtagcggaa aatctacgtt gatccaattg
    24841 atccctcgct tttatgatgt gagtgaagga aagatcttga tcgatggagt agacgtgaga
    24901 gattatcggc taagcaagtt gcgagataag attgggttta ttccgcaaaa agcgctgcta
    24961 tttactggaa cgattgcaga taatttgcga tacgggaaag aagatgctac gcaagaagaa
    25021 atggaacgtg cagctgagat tgctcaagct tcagaattca tttctcgaaa accagatgga
    25081 tttaatgaac atttatcaga aggtggttcc aatttttcag gtggacaaaa acaacgattg
    25141 gcaatagcca gagccatcat acgccgtccg gaaatctata tttttgatga cagcttttct
    25201 gcattagatt atcaaacgga tgcgaagtta cgtgctcgtc tgaaaaaaga aacgacagaa
    25261 tctactgtat tgatcgtagc acaacgtgtg ggaacgatca tgcatgcaga taagatcatc
    25321 gtattaaatg aaggagaagt cgtcggtatg gggacgcatc gggagttgtt ggaaaaatgc
    25381 ccgatttatt atgatattgc cgcttctcaa ttatcaaagg aggaattagc atgaaaaaaa
    25441 ccttttcttc cttaaaacgt ttagctcgat atatcagacc gtatcgtgag acttttcttt
    25501 tagtgatcct atttacagtt ctgacagtgg ctttcaacgt ggctttgcca tatgttacag
    25561 gacttcctac tacagaaatc agccgtaata ttgccaatgg cgaatcaatc aatttttctt
    25621 atatattcaa atgtctcatt tggatcacag ttgttggaat agggtattgt atttcgcaat
    25681 tactttctgg tgttttgatg acaaatgtcg ttcaaagtgc catgcatgat ttgagaagag
    25741 acatagaaga aaaaatcaat cgtcttccgg tttcatattt cgataaaaac cagcaaggaa
    25801 atattttgtc tcgtgtgacg aatgatgtag atgcagtcag cggtgcttta caacaagctt
    25861 ttattggtat cgtaaatgcg atattgggaa ttacgttggc tgcagcaatg atgttttata
    25921 tccagccgat gatggcgctg atctctatga tcatgattcc aacatcgatc tggatctcaa
    25981 aacgagtgat caatgcttcg cagaaatatt ttcaaagcat gcagaattct cttggtgagc
    26041 taaacggcta cgtccaagaa aatatgacag gttttagtgt attgaaagta tatggacgcg
    26101 aaaaagaaac attggaagga ttcaaaaaag ttaatcacag cttgaaatac tttgggtttc
    26161 gtgcagcatt catttctggc ttgatgatgc ctctagttca attaactgcc tatgctactt
    26221 atattggtat ggctgttcta ggaagctttt atgttattac tggggcgatc gttgtcggtc
    26281 aactacaagc attcatccaa tatatttggc aaatcagtca gccaatgggg aacgtgacac
    26341 aattgtcttc agttttgcaa agtgcatcag cggcaacgaa acgtgtattc gagattttag
    26401 atgagccaga agaaaaagaa aacaaagtag atgcttcact gccagaaacg attttaggag
    26461 atgtgacatt cgatcatgtc gattttgcat atgatccgaa gaaaccatta atccaagatt
    26521 tgaattttga agtgaaagct ggtcaaaccg tagctgtggt cggtccgacc ggtgccggca
    26581 agacgacatt gatcaatcta ttgatgcgtt tttatgacgt agacaaagga gcaataagga
    26641 ttgatggaat cgacaccaaa tcaatgagtc gcagtgatgt gcgttcgcta tttggtatgg
    26701 tcttgcaaga tgcatggctt tatcaaggaa cgattgcaga taatatccgc ttcggaaagc
    26761 ttgatgcgac agattatgaa gtagttgatg ctgcgaaaac agcaaacgta gatcatttta
    26821 tccgaacgat gccagatgga tatgatatgg ccatcaattc tgagggagac aatgtgtctt
    26881 taggacaaaa acagttgctg acgattgccc gagcagtggt ctccgatcct aaaatcttga
    26941 ttttagatga agcaacaagt tcagtagaca cccgtttaga agcgttgatt caaaaagcaa
    27001 tggatcgcgt gatggaggga cggacaagtt tcgtcatcgc tcaccgttta tcgacaatcc
    27061 gtgatgcaga tctgattcta gtgatggatc aaggtcagat cattgaaaaa ggaacgcatg
    27121 agagtttatt ggcacaaggt ggattttatg aaaaactgta taacagccaa tttgctgagg
    27181 aagcagaata agtcatataa gaataaaaca gtttaaaact tattttttgt tctctattct
    27241 tgataatggt aaagtaaagt tatcaagaaa agtgaggaca cggagatgga acagttttta
    27301 aatgaaaaaa ttactgaagc caaaagagaa gttgatcgtt taacgcacgt ctgtcagacg
    27361 aagccttttg attcagctga tttttttgaa aatgagtttc aaatcgaacg cttggcagca
    27421 caaatagaag tgtacgaatt aattttaaaa aaagtaatag aagaaaatat agatgtacaa
    27481 taaaaacagg taaaacgtca gttaccagat actgtaaata gacatctggc ctgagcaggt
    27541 tttacctgtt ttttttctta tgaaataaat atatgctatg atacctttat tcaataggat
    27601 tagtttctac aaaaaatgaa aggagaaaaa taaatatgga acggtttata gcatcaaatg
    27661 ccacagtgat tggtgatgtg acattgtcag aagatgtgac gatctggtat caagctgttt
    27721 tacgcgggga cagtaattgg ataaaaatag gacaaagaac gaatatccaa gatggtacaa
    27781 tcatccatgt cgatcatgat gcacctgtag atattgcgga gaatgtgacg gtaggacatc
    27841 agtgcatgct acatggctgt acaatcgaaa aaggggcgct gatcgggatg gggaccacga
    27901 tattgaatca tgcggttatt ggtgagaata gtttgattgg tgctggatca ttagtaacag
    27961 aaggaaaagt tattcctcct aatgtcttgg cttttggtcg cccagctaaa gtgatccgtc
    28021 ctttgacaaa agaagaaata caaaaaaaca aagaaaacat ccagcattat gtcgagatag
    28081 gtcggatgca tgcgcaagga aaattcaagg agcaaagata ggcagtagca tccataaaat
    28141 aaaaaaggat ggtcagtttg agttgaccat ccttttttat ttggtttaga taagagagct
    28201 taaaaaagct tcctttgaaa cagattaatt taatgaagaa ctgttttcca tgacttcatc
    28261 gatcaatcca tatgcttttg cttgttcagc tgtcatatag ttgtcacgat ctgtatcttt
    28321 ttcaatcact tcaagcggtt gtccagtgcg ttcagctaaa attttgttta agcgttcgcg
    28381 tgtttgcaaa atatggcgtg ccgcaatttc gatttcagtt gcttgccctt gagcgccgcc
    28441 aagaggttga tggatcatga tctcagcatt tggtaaagca aatcgtttac cttttgtacc
    28501 agctgtcagc aagaagcttc ccattgaagc agccataccc ataacaattg tttggacatc
    28561 tgctttgaca aagttcattg tgtcgtaaat agccatacct gctgtaactg aaccaccagg
    28621 agaattgata taaagataga tatctttttc agaatcttga gcgtctaaga aaagcaattg
    28681 ggcgatgatc gaattagcaa gatcatctgt tacttggccg cttaacatga tgatacggtc
    28741 tttcaataaa cgtgagtaga tatcgtaagc tctttcacct cgtgaagatt gttcaatgac
    28801 tgtaggaatt aaattcataa tagagttcct ccttgatttc gtttagaaat agtgtaccat
    28861 aatttagttt ctaataccta aaatgtagta tacatttttg gtcaaaaaag gtcaaataaa
    28921 aaacttaggc aaattagtcg cataaacccg ctttatggta tgataagcag agaaacaaaa
    28981 caaggaggaa caacagattg atagttggta ttactggcct tatcggagtg ctgacaggtg
    29041 ctgtagttgt ctatatcggc atgatactgc aaatgcgtca cttggaaaat aaagaacagc
    29101 gtagacgtga attggaattg aaagtcaaag aaatcgagac gctgaatcaa ttaaataaaa
    29161 agatcaacga gatcttgcaa aaacggacaa cgctgatgga agaatatgtc agctttgatg
    29221 catttgatga ttgttatatc acgattgacg attttgcgta tctccaatca ttttcagcgc
    29281 aaaatagttt ttatctgcca aattatattt tagacgagct atttaagaat atctcccatc
    29341 gaaaagtgat tttatctcca gatgaaacaa tgaagattgg tggatatgct tataaaggcg
    29401 gacgcgtgat tttagagaat tttacggata gtctgactga aatgatcaat gaaaaaaaag
    29461 ctcagttgaa gactatgacg cgccagccga ttggtttctt ctcgaaagat gatgtttgaa
    29521 gctgatattt tgaagcattg ccgttgcaca tgagagcgtg gtaatgcttt ctttttttct
    29581 taatgataca atagaactga acggagtgaa gaaagatgtt acaaatctat gcccatcgcg
    29641 gaagcagcgg cacccaccca gaaaatacgc ttcctgcatt tgcagaagct gtacgagtag
    29701 gtgcagatgg tatcgaacta gatgtccatc taagcaaaga tggttatttg atcgttatgc
    29761 acgacgaaga agtggaccgg acgactaacg gtaaagggct gattcgagaa aaaacattgg
    29821 aagaactcaa aaaattgaat gcagggggca aattcagcaa agaatttcca gcagctaaag
    29881 ttcctgaatt gagagaagtg ttaggactgc tcctccaaaa aaattaccga gggatgctaa
    29941 tgattgaaat taaaactgat cagtatgaat atcccggtat cgaagaaaaa ttagccgaaa
    30001 cattaaaaag tagagattgg ccttttcaac atgcatattg cagctttcat ttggattctt
    30061 tggaaaaaat cgcattactg gaaccggaga tacagctgga cttattgatg agtacttctg
    30121 ataaaaagat ccaactagca gcagaaaccc cttatataga aggagtacat ccaaaaaata
    30181 cttggttgtt cgaacatcca gtccaggcag ctgattttcc aaaagcaatt cgtgcatgga
    30241 cgattaacga tgaagaagaa atggccaaat gtcaagaact caaaatagat ggaataataa
    30301 ctgattttcc ggagaaagct cttcggtgga aacaatcaac gaaaagtagg taaaatatgg
    30361 aaaaagtatt agtgatcgtc ggtccgacag cagtcggtaa aacagcgcta agtgtggaac
    30421 tcgcacaact atttggagga gagatcatca gtggtgattc attgcaaatc tataaaaatc
    30481 tggatatcgg tacagcaaaa atcaaagaag aagagatgaa taatgttccg catcatttga
    30541 ttgatgtgat cgaacctact caacgttact cagccgctga ttttcagcat tcaggtcgac
    30601 aattgattac cgagattgcg aaccgagggc acctacctat catagctggt ggtacgggtc
    30661 tctatatcca atcccttcta tatgattatc agttaggagc gacagaagaa gcgaatcctg
    30721 agattagaaa aaagtatgaa acgttagctg aaacaattgg ttcccaagca ttatggaatc
    30781 agttgaaaga aaaagacccg gcagctgctg agaaaatcca ctggaacaat caacgcaaag
    30841 tgataagagc tctggaagtt ttagagacga caggacatag tatcacggca cctaaagaag
    30901 agccaaagca attgtatgat tacctcatga ttggattaga tacgaagcgt agtattcttt
    30961 atgaacgaat caatcaaaga gtcgatttga tgctagcaga aggtttagta gcagaagcgc
    31021 gtaaggtgta tgagcttgga gaagtccagg ccagtcaagg tattggatat aaagaatttt
    31081 tcccttattt tgaagggaaa gaaacattgg aagaagcaac agaacaagtc aaactacatt
    31141 cgagaagata cgcgaaacgt cagttgacgt ggtttcgtaa ccgtttggat gcacagtggt
    31201 ttgatcttat aagtgagcca aatcaaaaaa gtcagattga gcaaacaatc aaaaaatggc
    31261 tggaggcgga ataaaatgga agaaaaagtg atcatcgtag gggtagaaac cgaagcaaac
    31321 cagcgttact tttcagagtc aatggaagag ctagttcagc tgacgaatac cgcatcagga
    31381 gaagtcgtat tcacaattac acaaaagaga ccacaagtgg atcggcaaac gattatcggt
    31441 aaagggaaat tacaagaatt agttcaacaa gcggatgcgc atgaagcgga tttgatcatc
    31501 tttaatcatg agctaactcc gagacagagc caactgatta ccgatgctgt tggtcttccg
    31561 gtaattgatc gcgtgcagct tatcttagat atttttgcga tgcgtgctcg ttcgaaagaa
    31621 gggaagctgc aggttgaact tgctcagtta gaatacctgt tgcctcgttt agtagggcaa
    31681 gggaaaacat tatctcgtct tggcggtggg atcggaacaa gagggccagg tgaaacaaag
    31741 ttagaaacag accgccgaca tatacggaat aaaatttttg ctgttaagaa agaattgaaa
    31801 gaagtagaag ctcatcgaga gcgcaatcgc caaaaaagaa aaaatagcga gatttttcag
    31861 attggactta ttggctacac gaatgccggg aagtcaacta tcttgaatct attgacacaa
    31921 gcggatactt attcgaaaga tcaattgttt gcaacgttag atccattgac taagagatgg
    31981 cgttttgctg aaggatttga gattgctgta acggataccg tcgggttcat ccaagacctg
    32041 cctacgcaac tgatcgatgc tttccattct acattagaag aaagtcaaaa tatggatctt
    32101 ttgctacatg tagtcgatgc tagttctcca gatcgtattt tacaggagca gactgtctta
    32161 aaattgatgg atgaactgaa tatgaaagaa atgccgatac tgactgttta caataaggca
    32221 gaccagatcg acccagcgat gttcacgcct acgctattcc caaatgtatt gatttctact
    32281 cagacgaaag aaggtaagat ggcgttgatc gaagcaatca aacgtcagct gatggaacta
    32341 atgacccctt atacactttc tgtgccaagc aacgaaggac agaaattaag cgagttgaga
    32401 agacaaacaa tggttttgga cgaagcgtac atggaagaaa caaacgaata tgaagttaaa
    32461 gggtttgcaa aaaccaattc aaaatggtta agaaaaaacc aataaaattt tattttttta
    32521 a
//
LOCUS       DANJMA010000027        32304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000027 DANJMA010000000
VERSION     DANJMA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(126..1229)
                     /locus_tag="PWU31_001516"
     CDS             complement(126..1229)
                     /locus_tag="PWU31_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL1084066.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1451..1921)
                     /locus_tag="PWU31_001517"
     CDS             complement(1451..1921)
                     /locus_tag="PWU31_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL1084067.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2178..3497
                     /locus_tag="PWU31_001518"
     CDS             2178..3497
                     /locus_tag="PWU31_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL1084068.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3938..5578
                     /locus_tag="PWU31_001519"
     CDS             3938..5578
                     /locus_tag="PWU31_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL1084069.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5732..7054
                     /locus_tag="PWU31_001520"
     CDS             5732..7054
                     /locus_tag="PWU31_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL1084070.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(7116..7547)
                     /locus_tag="PWU31_001521"
     CDS             complement(7116..7547)
                     /locus_tag="PWU31_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1084071.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(7611..8447)
                     /locus_tag="PWU31_001522"
     CDS             complement(7611..8447)
                     /locus_tag="PWU31_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL1084072.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(8637..8939)
                     /locus_tag="PWU31_001523"
     CDS             complement(8637..8939)
                     /locus_tag="PWU31_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL1084073.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(8965..9396)
                     /gene="ruvX"
                     /locus_tag="PWU31_001524"
     CDS             complement(8965..9396)
                     /gene="ruvX"
                     /locus_tag="PWU31_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL1084074.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(9393..9659)
                     /locus_tag="PWU31_001525"
     CDS             complement(9393..9659)
                     /locus_tag="PWU31_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL1084075.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9913..10638)
                     /locus_tag="PWU31_001526"
     CDS             complement(9913..10638)
                     /locus_tag="PWU31_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL1084076.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(11004..11074)
                     /locus_tag="PWU31_001527"
     tRNA            complement(11004..11074)
                     /locus_tag="PWU31_001527"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11040..11042),aa:Gly,seq:ccc)
     gene            11191..11943
                     /locus_tag="PWU31_001528"
     CDS             11191..11943
                     /locus_tag="PWU31_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL1084077.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11959..12438
                     /locus_tag="PWU31_001529"
     CDS             11959..12438
                     /locus_tag="PWU31_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL1084078.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(12689..14269)
                     /locus_tag="PWU31_001530"
     CDS             complement(12689..14269)
                     /locus_tag="PWU31_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL1084079.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(14262..15005)
                     /locus_tag="PWU31_001531"
     CDS             complement(14262..15005)
                     /locus_tag="PWU31_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084080.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(15181..15957)
                     /locus_tag="PWU31_001532"
     CDS             complement(15181..15957)
                     /locus_tag="PWU31_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084081.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(16205..17041)
                     /locus_tag="PWU31_001533"
     CDS             complement(16205..17041)
                     /locus_tag="PWU31_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL1084082.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(17052..17981)
                     /locus_tag="PWU31_001534"
     CDS             complement(17052..17981)
                     /locus_tag="PWU31_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL1084083.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            18174..18527
                     /locus_tag="PWU31_001535"
     CDS             18174..18527
                     /locus_tag="PWU31_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1084084.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(18601..19257)
                     /locus_tag="PWU31_001536"
     CDS             complement(18601..19257)
                     /locus_tag="PWU31_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL1084085.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            19483..21417
                     /locus_tag="PWU31_001537"
     CDS             19483..21417
                     /locus_tag="PWU31_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1084086.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(21503..22726)
                     /locus_tag="PWU31_001538"
     CDS             complement(21503..22726)
                     /locus_tag="PWU31_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1084087.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(22720..24618)
                     /locus_tag="PWU31_001539"
     CDS             complement(22720..24618)
                     /locus_tag="PWU31_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL1084088.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(24612..25730)
                     /locus_tag="PWU31_001540"
     CDS             complement(24612..25730)
                     /locus_tag="PWU31_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1084089.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(25813..26646)
                     /locus_tag="PWU31_001541"
     CDS             complement(25813..26646)
                     /locus_tag="PWU31_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084090.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(26639..27556)
                     /locus_tag="PWU31_001542"
     CDS             complement(26639..27556)
                     /locus_tag="PWU31_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084091.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(27783..28907)
                     /locus_tag="PWU31_001543"
     CDS             complement(27783..28907)
                     /locus_tag="PWU31_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1084092.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            29090..29998
                     /locus_tag="PWU31_001544"
     CDS             29090..29998
                     /locus_tag="PWU31_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084093.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            30139..31752
                     /locus_tag="PWU31_001545"
     CDS             30139..31752
                     /locus_tag="PWU31_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1084094.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT         9981 a         6412 c         5502 g        10409 t
ORIGIN      
        1 agtatgaaaa aatatttcaa ttttattttt tcgtttcaaa ttattctatt agtgacatcc
       61 ttgaactata aaaataggag tacggcatca tctgccgcac tcctattttt ttcgtcattt
      121 gtttcttaaa taaaacgatt ctcacgcaat tttatttttt tgatatcttc tactgtttgt
      181 gttccagcta attgcatgac catttccaat tctgttttaa agaaatcaaa cacttgatgg
      241 actcctgtac ttcctcctaa agacaatcca taaataacgg gtctgccaat agcaacaaga
      301 tctgcacctg atgcgattgc tttgaaaaca tgctgtccgc gtcgtacacc gctgtcaaac
      361 acgattggta cacgtttatc tactgcttct gccacatatt gcaacgaatc aaacgctgct
      421 ggaccaccat ctagttgacg gccaccatga ttagataccc agattccgcc agcaccagat
      481 tctaatgaac gatagacatc ttcttctgac tggacacctt tcacatagac tggcaaatca
      541 gaatgagcag caataaattc tacatctttt ggactaagct tctgtttgga agatttgtac
      601 acagcatcca tcgtttggcc tactcctgat tgataagcct gaacgatcgg cattggcagt
      661 gggaaggtaa acccatttct gcggtctgtt tcacggtttc cgccaactgt tgcatccgct
      721 gtcaatacga ttgcttttgc tccattacgc ttggccactt caagaatatc caaattgatc
      781 ccatcatctt tgctcatata aaattgaaac cattgcggtg ctttctcccc gcctgcttct
      841 cttatctctt ctaatgtaca agaagcgtaa gaacttgctg tatagatcgt cccaaaatct
      901 gctacacctt ttgcagaagc tttctccgcc ttcacatgag ctaagccatg agcagcaact
      961 ggtgccatga tgattggtgc agtcagcgtt tctttgtcaa aatgtgtagt tgtatcgggt
     1021 aattctacat ctcttaatac atgaggaata atcagctgat gattgaatgc ccgttcattt
     1081 tcttgataag taaataagtc gccagcacca ctgctgatgt aaccataacc accagctgga
     1141 atcacttgtg aagcggctat ttctaaatct tccatattga taaaatccac tattccttca
     1201 tttgtacctg cttgatacgt tttttccatt tcaatgcctt ccctctataa aaaattgaat
     1261 gaataatatg atagcgttta cacactttat attactcttc cgtattgata caggcaaata
     1321 atctgtttac atttcagtca ttccagttgt tactactctg aaagaaatgt ttagaacaca
     1381 aatagataaa aaacgatggg attttgacaa aagtcgtgtc aaagtccctt ttacgaaata
     1441 ctgtggatgc ctattgaaat aagtgcgggt tgtttctttg tatttcaatc gtttcttgcc
     1501 attcttcgtt taattttgga ttaccgtttt cttccttgaa cgcataaggt cccaatccaa
     1561 aaagctcagt tagtatttga ttcacgattc catatagctc ggtgtgatcg gacacatgat
     1621 tattgaagat catctcatta tcaccacgtt tgaatctcac attcgggctc tcatgatgaa
     1681 cctctatgca gttttcccct ttttttaact gacggatttc tgaaaacatc tcataaatat
     1741 cttctccaat aaaaccttca gcaagcgcca gactgtccac aagaccgcct actgtcaact
     1801 cagagattat cgctttgttg gcaccaagct ttaatgtata gcttgttgct tctactacgc
     1861 cgcatgcatt gcaaccacct tctgatagtt cgctttttct gatcaattga acttctttca
     1921 ttttctgttc ctcctcttag ttttctactt atgtattttt tatcacctga cgtaagtgta
     1981 ttttatcgtc acgatcacgt gatttattct tcagagattc tacctgaata attcatactt
     2041 ttaattaaaa ccatgtgaaa ctgcctcacg gcagcttaca attacttttt catcttcacc
     2101 cgttaagtga tgaaaaaaga acccctttct gctatggtta aagtgactaa accaaccaaa
     2161 agaaaggagt tcttttcatg actagcttac acaaaaacca agtaaaattc aattcaaata
     2221 tcactatttc tcatacaggt ggtcaattat cgagtgattc gggtctcgtc ctagtgaaag
     2281 agctgatgaa catctttggt ttttctgaac tggctaagca acacattcac attgaagacg
     2341 aacgagcgta ttttactcat gacaacttat ccatacttga gcagttcatt atgcaattaa
     2401 ttgctggtta ctcagctgat tctgcagcta atctcctgag acaagatcct gtgtttaaag
     2461 ctgttttaga taggaaagaa ctcgcttctc aatcttcact ttcccgattt ttagatcgac
     2521 tatctgagga gaatatccat gaacttcaag ctttgaacca agaacttatc gataaagcac
     2581 gcctcatccg taatgatacg gaattaatca ttgatttaga ttcgacccat tcagatacat
     2641 ttggtcacca agaacaaacg aattataata cacactacca aacctatggt taccatccat
     2701 tggtagcttt tgacggattg actggtgact tcttaaaagc tgaactacgt tcaggaaatc
     2761 aatatacatc aaaaggcgta aaggagtttc tcacaccttt attagagcac tataatcact
     2821 ctctaccaaa cactgacatc ttggttcgtg gagacagcgg gttcgctaca cctggtgtgt
     2881 atgattcgtg tgaatcaaaa aagagtcatt atgttattcg actgaagaat aatcgtagac
     2941 taggtcaaat agctgagaaa tcagtacttt atggcgataa tcaaaagtgg gaagaacgag
     3001 aagttcagta cttctcgacc acttatcaag cacaatcctg gtcacaaagt cgtcgcgtgt
     3061 gtatacgctc aacacgtgaa gcgggcgaac tactctttcg acatgaattt atcgtgacga
     3121 atctatcaga aaatgtttct cctgatacca tcttttctat ctatgccaaa cgtggcacaa
     3181 tggagaactt cattaaagaa gcgaaagctg gcttttactt tgacaaaaca gacagtcctc
     3241 gctttttgga gaatcatgtc cgaatgatgc tcagcttgat agcttacaac ttagtcaact
     3301 tcttaagaac gattggcttt gaagaagtcc aaaagggaat gaccattcat tctattcgat
     3361 tgaagtttct gaaagttgct gggaaattag tccaaacggg tagacgagtc tatctcaaat
     3421 tatctagtta tcatgtgtat cagaatgaat tctacagggt ctttgctcgc ctgaggcgag
     3481 ccagtcaatg gatctaatcc aacaatctaa cgattttttt actgggaagt tatccaggga
     3541 gaagtatgct caaaatgcct tacacccaaa atttattcct caatattttt gaacagtgaa
     3601 tgtttgaagt aactttttag tcaaaatcag ttgataggga tattagtgat acatatttca
     3661 ataaaatgtg ccaaagaatt aaagctatga attattcagg attctattta tctatctaac
     3721 atatccttta ttcttctact tttctttctt tagcatataa gagatacgca tagtaatcaa
     3781 gcaaggattg ttgatataaa aagaacaaat accgctattt tgatttcttt tgactctatt
     3841 actcatataa ataaaattta ttatacctgc tctttttgac tatatcctca tctgactcct
     3901 atacttcgtt tgtattaaaa cttaggagga atcattcatg cttacagggc taaacttatt
     3961 aaataatcct tttctaaata aaggaacagc ttttacaaaa gaagaacggg caaaatacgg
     4021 aatcactggt atgcttccaa gtactgtcca aacattggaa caacaatctg tccaagctta
     4081 cggtcaatac ttaagcaaac agaccgattt ggaaaaacgt atcttcttaa tgaatctatt
     4141 caatacgaac cggacattgt tttataaatt gatgggcgaa catttagttg aattcatgcc
     4201 tgtcgtttat gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa
     4261 accacaagat gctgtatttc tttcaatcga cgaaccagaa aatatcaaag caagtttgaa
     4321 aaacgcagca gatggacgcg acattcgttt gatcgttgtg acagatgcag aaggaatctt
     4381 aggtatgggt gactggggtg tgaacggtgt tgacatcgct atcggtaaat tgatggtcta
     4441 cactgccgcg gctggtatca atcctgcaca agtcttgcct gtatcaatcg acgctggtac
     4501 taataacgaa gaattattga ataatccatt atacttaggg aatcgtcatg ctcgtgtaga
     4561 aggcgaaact tactacgaat tcattgatca attcgttcaa tcagctactg aattattccc
     4621 agaattgtta ttacattggg aagattttgg acgaggaaat gctgcaacga tcttagaaaa
     4681 atacgaagat aaaatcacta ctttcaatga tgatatccaa ggcacaggaa tcgttgtact
     4741 agctggtgta ttaggtggat tgaatatatc aggtgagcaa ttgaaagatc aaacgatctt
     4801 gacatttggt gctggtactg ctggtgtagg tatcgcaaac atccttttag atgaaatgat
     4861 ccgtcaaggt gtacctgaag aagaagcacg ccaacatttc tatcaagtag ataaacaggg
     4921 tttattgttt gaagatacaa aaggattgac tcctggtcaa atcccattcg ctcgtaaacg
     4981 ctcagaattc actaatgctg atgaattgac aaacttagaa gcagttgtaa aagcaatcca
     5041 tccaacgatc atgatcggta cttctaccca acctggagca ttcactgaag aaatcgtgaa
     5101 agaaatggcg gctcataccc cacgtccaat catcatgcct ttgtctaatc caacaaaatt
     5161 ggcagaagca aaagcaaaag atttgattga atggacggac ggaaaagctt tagttggaac
     5221 aggtatccct gctgctgatg tggaatataa cggtgtaact taccaaatcg gtcaagcaaa
     5281 taatgcgttg atgtacccag gtttagggct tggtttgatt gcttcaacag ctacacgagt
     5341 aaatgctgaa atcatttctc aagctagtcg agcattaggc ggaatcgttg atgtaacaaa
     5401 accaggagca gctatcttgc ctccagtagc aaaaatcact gaattctctc aaacgattgc
     5461 tgaaactgtc gcaaaatcag ttgttgcaca aaaattgaat cgtgaagaaa tcacggatat
     5521 caaagaagct gttgaatctg ctaaatgggt tcctgaatat aaatctttag aagattaaga
     5581 ttgttatagt aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact
     5641 gcggcaaaac agcagccagc caactactgc tcttttgccg cttctctttg gcttttttca
     5701 tacgatacag aataagaaag gatctgtaaa aatggcacaa aaaacaacaa ccgcaactgc
     5761 agttgaggaa aagaaaaaag aagggttttg gtcaacaaaa attaccggcg taagtttgcc
     5821 cctttatttg atcatggtcc ttgtcctgat cattgcaatg gctttaggaa aattaccgac
     5881 gaatatgatt ggcccaatcg ctgttttggt cattttcggt aatttatttc attttatcgg
     5941 aacaaaaatc cctatagtca aaagttattt aggtggtggc tccgtttttg cgatctttgc
     6001 ctctgccgcg attgctacat ttgggatctt acctgagtat gtagtaaaat ccacagaaaa
     6061 ctttgtcagc aatatgggct ttttagactt ttatattgct gccttgatta ctggtagcat
     6121 ccttggtatg aaccgtaatc tattgatgaa agcttctgtt cgttttattc ccgttgcact
     6181 tatttcaatg gtcgtctcct tttttgccgt aggactcgta ggtatgctta tcggaaatgg
     6241 ttttgcaaat tccgttttat atatctcatt gcccgcaatg gctggtggtg ttggagccgg
     6301 agctgtacct ttatcaacga tttatgcaaa tgttttaggt acggacgctt cttctatcat
     6361 ttcccgcctg attcctgcga cagcaatgac aaatgtccta gctatcattg gtgcagcgtt
     6421 agttgctcgt gcgggacaat caatgccaaa attcaacggt aatggtaaat taatggaaaa
     6481 aggcgacctt ggcgatggaa aacctagaaa tgtaaaacct tccattccac aattaggtgt
     6541 cggtttgatg gtatcattga ctttcttcat cttaggacaa atctgtaact attttgttcc
     6601 taaagtccat gcttacgcat tcatgatcat tatcgttgtt atctgtaaaa tcgcaaacct
     6661 attaccagaa tattacgaag atgcagctat catgttcaac aacttgattg tcaaaaactt
     6721 gacagcagct gttcttgccg gtatcggtat tgcgttattg aatctaaatg tattggcttc
     6781 tgctttgaca tggcaatttg ttgtcttatg tttgacaagt gttatcgcga tttcagtagt
     6841 ttcaggattt gtcggtcgtt tgtttggttt atatccaatc gaatcaacta ttactgctgg
     6901 cttatgcaac aactcgatgg gtggtacagg aaatgtagct gtcttatctg cagctaatcg
     6961 gatggaactt attgcttttg ctcaaatggg caatcggctt ggtggtgcca tcgttttgat
     7021 catctcaggt ttcttgatgc aattactttc ttagtcaaaa aaaatacctg atcatcaagt
     7081 atcggcttga tgaccaggta ttttttgata aaaatttaca ggattttcaa aaatcttttt
     7141 tgcattttat cggaatgata accgatgcgt tcataaaatc ggtgtgcttc tactctgtat
     7201 tctgcagaat taagtcttat tgctgatatt cctcgtcttt tcgcttcttc tacgccagtc
     7261 atcaaggcct ttccgattcc tcttttttca gcatgtttag acacagcaag tgccataata
     7321 ttgaacatcg gatcgctgaa aatcgattca taggactccg cttgtacata gccagctgct
     7381 tttttagaag taggatcttc atagaccaag aaaaaatttt tttctggctc cttcaacagt
     7441 ttcttcattt gtctcaccgt aacggttaat ggtacatcat atcctaattg ttccgcattg
     7501 atttctcgta gtgcttctgc atctcctaaa cgcatttccc gcaacatcca aatcaccagc
     7561 caatttttat ttttttaaat gaattttttt atcacagtat actccttttt tcacttaaaa
     7621 aaagactttt cagtgatttt gacttgcttg aatccagatc cttctaatgt ttgcaagatg
     7681 acgaaagcat tgtaaagatt gatcaaaaga accacgatga caatcgcttg aaaaataaag
     7741 gtcacttttt ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca
     7801 gcagcgggga taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa
     7861 gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt
     7921 gcttctttta ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca
     7981 ccaataccta aaaaactagc taaaaagccc agaacccccc cacatataag gtaccaagtc
     8041 catgacgtca aatgaaattt tggctgatga tgcttggtgt agaagaacgc aaaaaccaag
     8101 gtcaataccg tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc aaataactgt
     8161 aaaaaaactt cgaatacaat gttgccgact actcctccaa caacagctcc actagatacc
     8221 caaccgacaa tctgccaatt cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca
     8281 atggacatcg tgaataccgc aactgttgag taaaaagaaa tccctgcaac agaatgggcg
     8341 ccgataagat caagaatcgg tttgatcaac acgcctccac ccatcccgga aatagcgcca
     8401 actgtgttcg ccagaacgat caccacaaaa taaatcagac caatcatctt gtatttcctt
     8461 tctcttgttt atatctttta gcgattgtta ctcttttctt ttattataca gtaaaagaaa
     8521 caaacgaggc ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa acgaaaaaac
     8581 cgggctctac ttttcagaga gtccagcttt tcttacacct tatcgattct tttgttctat
     8641 tcttcttccg ccatgaatgt attgaatact tcttcgatca tatcccattc tgcgtctgtt
     8701 tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt
     8761 aattctacat cttcatcttc tgaaacacca gctggatata aaagaacata gttacgtcca
     8821 aattcttctt gtccgtcaac tgttagcaag atttcgtata atgtttcatt tccttggtca
     8881 tccaccaatg tgatgtgttc gtgtccttcg tgttcgtggg aatggttatg ttctgtcata
     8941 agattacctc ttttcttttt attattaaaa taattgctta cctgagccgt ctaaataatt
     9001 ttgtaaaatc atcacagctg ctaatttatc gatgactttt tttcgtttcg ctcttgaagt
     9061 atttgcctgc tctactagca tacgttctgc ctgaacagtg gttagtcgtt catcttgata
     9121 atcaaccggc aaggagaata gttctttgat cttctctcca tatgacatgg aagcttctgc
     9181 ccttggtccg atggaattgt tcatattctt aggtaatcca actacaaaac ggtctacttc
     9241 gtactgcgcc actaattcct tcaaccgatc aaaaccaaac tcttcttctg cctcattgat
     9301 tcgaatgatt tctactcctt gagctgtcca gccaaaggga tcgctcacag caacgcctac
     9361 tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg
     9421 tagtctttga caattacttc catgatttca tcgcgttcgt gacggcgaat caaatttcgt
     9481 gcatcacgat aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga
     9541 ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc
     9601 acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg
     9661 attctacacc tctttcccgc aaggtaattc cagcttacct tctacaccct tattttactc
     9721 gaagaaaggg aaaactacaa acaaatattc caaacttctg aataaattaa gaaacacgat
     9781 ttgtctgatt tttccttttt tctgtggctt tttcaaaagc cctctttcca taaagtatac
     9841 gctatatttc tttgaaagta tgtttttaca caattttttc ataaagttca taaaaaattc
     9901 gaattgctgc tactaatttt tcgttttcgt tgcttgccct gtctcataat tgatggtgta
     9961 gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc
    10021 tgcttccaac tgcacacctc ttgctaccag ttcatttccc tcgaaatgag gagtgacacg
    10081 atagcgtaca tgatgatttg tttccttgat atatgccgca atatcatttt cgtgggcaag
    10141 catgtacgga gcattcaacg aacgtgtccc tgtcattaag tttttaatgt tattattttg
    10201 accagtcaac tggaaaccaa tcaaatggct gcggttgtat aaccattgtc cgtcacttaa
    10261 tttcttttgt ttccaacctg tcggatcaat atacaaaggt tctctctttt cagtaggaaa
    10321 caagtctttt cccaacatcg cattggcttg tcctactctg ttcaatttgt ctatatttga
    10381 atagctttcc catgtccctt tggaaagact aagatcctct tttgtaaact ccggctgatt
    10441 cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga
    10501 agcttgcggt gtggaatcaa aaatggttgt aatttgttgg aagatatctg aatctttttg
    10561 tgtggtggaa ataccgagtg cccctgcaat cacagcagcg acgatcccca cgatcgcctt
    10621 ggttgttttt ttgctcattt ctttactcct ttactaacag ttccctaaat cttaacatat
    10681 gttcccctat aaataaatac tggaaagaaa tagaaacagt aaaaaaataa aaccagtttt
    10741 tagacggctt aagaatacaa aaaaagagac ggattcatcg tctctactca atcacaaaac
    10801 actatttaca aggtatttat tatcattcaa aaaatttttc ttaatactca aaactaaacg
    10861 gcttctgtac gacctcttgg taaacagtgt tcaaaagctt gcatctgcgg taataagtcc
    10921 aaccaagtca aaaacataag aagctttttt gcttgactat tcttattact tgatgcaggg
    10981 cagctttttc acagcctctt ttaagcgggt gacgggaatc gaacccgcga catcagcttg
    11041 ggaagctggg attttaccac taaactacac ccgcgataca aaaacagtat acgcctcttt
    11101 cactccttcc tcaagttttt ttattcttta cgcttccgat atgttttgta gtaaaataaa
    11161 actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga tctgttgctg
    11221 gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat gtatcgggta
    11281 gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt cagctatgtc
    11341 actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat ggaacaaatt
    11401 accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc acgtaaatat
    11461 gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat cgaccgagac
    11521 cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt
    11581 atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat tggaggattg
    11641 ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt atcggaattt
    11701 ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg cttaggaagc
    11761 aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt tcaaattacg
    11821 aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg cggaacagaa
    11881 gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca gcttttttat
    11941 taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag ttttttagcg
    12001 actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg ttgtggtctt
    12061 tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg atctgctgct
    12121 tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt ctctaaacgt
    12181 ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt ccctggtggt
    12241 ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc ggctcattat
    12301 gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt atcagaagtc
    12361 tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa
    12421 aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc
    12481 cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg agcaataaga
    12541 agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt ttacataaaa
    12601 ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa aggggctgcg
    12661 acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac gttcgttgat
    12721 aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga atattcacta
    12781 accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata atactgatga
    12841 tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg ttaaataatt
    12901 ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa taacgtagtg
    12961 acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa aacaaaggca
    13021 gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc tgcaatatcc
    13081 atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa gaacggataa
    13141 acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc tgattaactg
    13201 ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta ttatctaaat
    13261 cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt ggagttaaca
    13321 aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga gcattgtaac
    13381 taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt ttgaactcac
    13441 tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt acagcaagga
    13501 aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt tgagaaagtg
    13561 gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga tctgttaact
    13621 cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat gcgatcagca
    13681 tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac actgctgttc
    13741 gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa aatagagaaa
    13801 acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc catccggtta
    13861 aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa
    13921 tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact tgcaggtcat
    13981 ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct aacataccaa
    14041 aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata gcaaacatag
    14101 caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac aaaagataga
    14161 tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc tgtggattaa
    14221 catataaaag attaactcga attaattcta ataattgtcg cttatacata cggtccatct
    14281 cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc atcaggtgag
    14341 ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt caaaatagcc
    14401 agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat gactgttttt
    14461 ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg aggatcaagt
    14521 ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt gccgatcaaa
    14581 atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg atgactatac
    14641 atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt gtctaattca
    14701 taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa aatatctggg
    14761 gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt caatttcatt
    14821 ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact tttaatcgta
    14881 gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg tcttatcgag
    14941 agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt gatgaattca
    15001 atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg ttattggtgg
    15061 agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc tgctacttgg
    15121 gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc tttgttttct
    15181 ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat caatacttct
    15241 ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta ctctgctaat
    15301 agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata aatactcata
    15361 ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa
    15421 tactaggcct attttatttt tataacctgg catgctcatt agataaagga tttcatcgta
    15481 atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg
    15541 ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt
    15601 ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat taataacagt
    15661 tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct
    15721 aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa
    15781 aatatctgag gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa
    15841 tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc
    15901 tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa
    15961 aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat
    16021 gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca
    16081 aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa
    16141 aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt
    16201 tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg
    16261 taacaactac agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc
    16321 cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca
    16381 aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat
    16441 ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa
    16501 tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga
    16561 tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt
    16621 ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat
    16681 cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt
    16741 cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag
    16801 cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt
    16861 cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata
    16921 aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc
    16981 cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca
    17041 tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc
    17101 agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg
    17161 accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa
    17221 gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc
    17281 atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa
    17341 taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa
    17401 aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt
    17461 tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta
    17521 gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc
    17581 acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca
    17641 aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa
    17701 gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg
    17761 ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata
    17821 cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct
    17881 gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc
    17941 gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt
    18001 tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaaacaag ttgctctgta
    18061 aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt ctgttattcc
    18121 cattaaatta aggtctcata catatcactt ctatcataaa agggagaata aaaatgaaac
    18181 aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt gccgtcattt
    18241 atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta gaggaacttc
    18301 tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca gaagtagcta
    18361 gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga aaagaagtct
    18421 ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat gaactgacag
    18481 cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac
    18541 agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg ctttttattt
    18601 ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt
    18661 ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag cattcaatcg
    18721 agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat tgactacttc
    18781 ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga tcttgatttg
    18841 ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc caacgatttc
    18901 ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga atttgtattt
    18961 tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca attgatcgtc
    19021 gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat acccacgttt
    19081 tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta cttgaacggc
    19141 ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa tccttaaata
    19201 gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat ctttcacgaa
    19261 atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca gtatttatag
    19321 gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc atttatttat
    19381 catttctgca aatctcgcag tataatggaa tttatggtag actactaatt gaaaaatagt
    19441 tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt attacaagcc
    19501 aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca tttagaaatc
    19561 agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc gactttatta
    19621 aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg
    19681 actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt ttggaacgag
    19741 atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga gctagagatt
    19801 ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa agaatacgac
    19861 cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga aattcgttcc
    19921 gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga tactttatca
    19981 ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc tgacattttg
    20041 attctagacg aaccaacgaa ccatttagat atcgagacac tttcctggct tgagaattat
    20101 ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt tttagataaa
    20161 gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa aggtaactat
    20221 tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc ttatgaaaag
    20281 cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt tcgcgcctct
    20341 accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg gctggatcgt
    20401 cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc ttctggaaat
    20461 gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt atctgaacca
    20521 atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa tggtatcggg
    20581 aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg aaaagaaacg
    20641 ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct gcatacaaac
    20701 aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa agagatccga
    20761 agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat ccctctgcta
    20821 agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa agaaaatttt
    20881 ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt attagaaaat
    20941 gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata tttcatcaat
    21001 cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta tctaggagac
    21061 tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa
    21121 gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca aagcaaagaa
    21181 cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga agaaatggcg
    21241 cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga aaacctggaa
    21301 gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca agatcaagaa
    21361 acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga aaattaacga
    21421 aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat tttatcaaac
    21481 aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc attctcacta
    21541 aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca ttatttttgg
    21601 gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta gcatatcttt
    21661 ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt tctgttttgt
    21721 ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc atactagatg
    21781 tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa atggtatctt
    21841 ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa tatttttgtt
    21901 ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc
    21961 aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt ccatctttga
    22021 catccatata aaatgtatga taagttgaac catcttctct tactaaataa tctgctgcat
    22081 tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct tcactcgccc
    22141 aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt tgtgaaggat
    22201 cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg
    22261 tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt ttataatctg
    22321 ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata atttgacagt
    22381 caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat aaaggatcac
    22441 ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc caaccaatat
    22501 tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga tactgactgt
    22561 tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc tcattattcc
    22621 attctttttc cattaattct ttataacatt catttatcca tttttctgga tgtattatct
    22681 cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt ttagtctttt
    22741 tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa ttttgggacg
    22801 attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc ctgttccgag
    22861 ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt tttcccatgt
    22921 catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa tagtatcgtc
    22981 acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt ccttgtcttc
    23041 ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg tctcatatag
    23101 aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt caaaaggaat
    23161 aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa gtacctcttt
    23221 cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg ttttgatgct
    23281 ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta gcattgaatc
    23341 cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa attgactaga
    23401 ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc ctaattgtcc
    23461 cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg cttctgaaag
    23521 aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat actcatggtc
    23581 ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata acgtactatt
    23641 atagggttca gtcaatgtca attgagggta ggtataacca atattcagga tacctttttc
    23701 atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc ccttgatttc
    23761 acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc ttgaaactgc
    23821 aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg caaaaaaatg
    23881 aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa tgggcttttc
    23941 tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat tataataatc
    24001 ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat tttctatatt
    24061 atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt ggataattgt
    24121 tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt caaaaatttg
    24181 cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc ataataattg
    24241 ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct ggtctctttc
    24301 atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta ttcctgatag
    24361 aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc cccataaaac
    24421 ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt gatgtatata
    24481 atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta tctttttaaa
    24541 taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca ttgtattttc
    24601 tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa gccttcaata
    24661 aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg gtatgcttct
    24721 gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg cttttctgtc
    24781 ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga ggaaacatat
    24841 cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc aatattcgaa
    24901 tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt ctgaataaaa
    24961 tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta acgataactt
    25021 aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt ctcattgtat
    25081 ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc tgcgatatgc
    25141 ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg ctgccgagca
    25201 attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa tggtaaattc
    25261 atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac taaatagttc
    25321 cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag ttgtcgacta
    25381 tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa aaaacctaca
    25441 tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc ttgttgtatt
    25501 gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa aataccactc
    25561 caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt atattttccg
    25621 ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc tatcttttca
    25681 gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat ggatatcccg
    25741 ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga tggttaacat
    25801 tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt tagactgcgc
    25861 ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca taattgcgcc
    25921 ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta agggaaccgt
    25981 atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc aacttcccat
    26041 aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag cgattttcgt
    26101 aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg ctgtaggaaa
    26161 ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg aagggatgat
    26221 cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata aaggaatcaa
    26281 tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca gtttattccc
    26341 cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg tagctgaaac
    26401 tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat tatatctcgc
    26461 catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga ttccgccact
    26521 taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg gcatcaaaaa
    26581 tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta tcttttgttt
    26641 agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca gatacaaaca
    26701 ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa cttgttttat
    26761 ataaattgac tcctacgacc aaagctgaat tttctggtcc attggaagtc ccaaagaata
    26821 tatttgggat attgaaatta cccaagccat tgatagtggc taaaactgtg gccattaaaa
    26881 tgattggctt tagcatgggg atcgttaata aaaagaattg ccgaaatttc gatgctccat
    26941 ctatttcagc actttcgtat atctctttag aaatcccttg aagaccagct aaaaataaaa
    27001 tagtgttata tcctacacta agccaaatca tcgtgatgat cattgatatt cgtatccaaa
    27061 aaggatttgt taaccactgt acaccgttaa ttcctagcgc attcaaaaat ccattgaata
    27121 acccagtcgg attgaaaaaa gtcatgaata cggaagctac agccaccgtt gatgtaatgg
    27181 ttggaatata atagacagtt ctaaaaaatc cttttccttt taactgtgct gaattcaata
    27241 aaacagcaaa gtaaatagct aatagcatcg ttattggaat tgatcctgcc atgataatca
    27301 aaacattcgt gtaagattta ataaacaatg gatctcggaa gatagctaga taattgtcaa
    27361 atccaacaaa ttcaaatgag tagccaattt tcataaaact aaaagcaaac gagataatga
    27421 tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat ccataagcac
    27481 tttttttatg agacgtatct gttgcttttt tcttgctgtt ctttattgtc ttgcttggta
    27541 acgaagattg catcatcgtt aagtcctcct aatatttttt aatcaccagt taattttacg
    27601 ccgtacttat gagcaaaatt ttctgcttct tttagatatg aattttcaaa atcacctgtt
    27661 tgccaatagt tatatgcagc ggtagtcaaa taagacgtag catctgaata agtggctggt
    27721 aaaacagtat ttgatgtatt ttcattgatt ttattcaata gttcatagta tttttgtcct
    27781 ccttagtagt caaacttttt ctcagctgct tctgtttgat aggccaatga gttagatgta
    27841 gccacgccca gctccagtgc gttggctaag cattcttcat ctgtcagcat aaatgtaaga
    27901 aattgttgtg ccacttgtgg atttttggca tctttaggga cataaaaact tgatcctcct
    27961 gatactgcac cttttcctgg attttctttt gaaaaaggaa tcagtggagc catgcgccat
    28021 ttgtcagatg catcaggaaa attcaaagac atattcgtag cataccagcc gccagcaaca
    28081 aacattgcag attcttgaaa tgctttctca gcatcttgtg caccgtagaa atgtacaata
    28141 tctgagtcga tcatttcttt gattaacttt gcagcatgtt tcgcttcttt agtattcaga
    28201 cggatatttc cttcttcatc aagcaaagat tgattctgct gggctagcat gttgataaac
    28261 aattcttggt ccccagattc tggcaaggca attccaaaac taccagtttt ttcttttaat
    28321 gcttttgcca cctggatata ttcttcccaa gaactgattt cttcaaaatc tacacctatt
    28381 tcagcaaatt tttttggttg ataataacaa agtgctgggg taaaatcact agcccaagga
    28441 ataatcgttt cattactcgt ttgttgacgc aaaatattca atttttgatc ataaaactga
    28501 tcgccatatt tgtcataaaa tccatattca tcagcactat aaaaagcatc ttgaaatttt
    28561 tccagcacac ctgctgctga agtgtctgcc atggagataa tatccggcat atcttcgtgt
    28621 gttaccaatt ttggggtgat catttgtact gttgctgttc ccgaagctac attcacaatt
    28681 tttaagtcaa tatttgctcc agttttgcgg ttatagattt ttgcagcatt ttcaacatag
    28741 tttgtcagtt tgccgtttgc gacatatact tctaatgtag tggctttttt tatcattgga
    28801 tctgcgatgt ttgctgttac ttcttcgtta gtttttccac atgcacttaa gctgaataat
    28861 agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc tatcccttct
    28921 ttcttttcaa gttcttatat aaaacgcttt cacttatgta tattaacgga attagtaaca
    28981 caaatattta tataaaaaaa tattttactt gtcgttttgg cttttcattt ttgttataat
    29041 agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag tggttctttt
    29101 gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt ccatagatac
    29161 ccatcttcat ttatctgctg ctccctattt tcgactacta ataaatgaaa gcgctaattt
    29221 atatgctgaa tttgcacata aatctataga actagataat ccttcccttt tattcattga
    29281 taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa acacaaactt ttatcattat
    29341 ccagttttat tttgatgagc aattattagc acatattacg ggacaatctt tttctataga
    29401 tcaagaaata acatctatca ttacttttct gaaaaatgaa cagttcaaaa ttgattatct
    29461 aaaacatagt aagccagcta tttcctatga tcaggaaatc aattcttttt tcatactatg
    29521 ccaacagatg agcattcatt tgctttcctt attagttact tattgtctgc aaaaaattca
    29581 attgtcttcc ttaccaaggc attatcaaca ggaacttgct ttattgacaa taccttcaaa
    29641 tgagaaagtc attcctaaaa aaaagcatat tctttattca gatgaattag ttgatccaat
    29701 catacgatat ttagaagaac atctttcaga aaattttttg cttgaacaat tagcccaaat
    29761 tttttttatc agtccagcca gcttaaaaaa acattttaga aaagtgaatg gaaaaagtgt
    29821 tattagctat tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc tagaaaatga
    29881 attaacctat acagaaattg cagatatatt aggatttcat tcgatccatc atttttcttc
    29941 agcttttaaa aaatatacag ggttatcccc ttcaaagtat tatctatcct taaaatagaa
    30001 aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa
    30061 acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt
    30121 agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca
    30181 tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga
    30241 gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac
    30301 cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc
    30361 tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac
    30421 ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct
    30481 agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt
    30541 agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc
    30601 gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc
    30661 ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt
    30721 gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga
    30781 gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct
    30841 gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc
    30901 ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg
    30961 ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg
    31021 atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta
    31081 aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat
    31141 catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat
    31201 tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga
    31261 ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt
    31321 ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg
    31381 acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt
    31441 gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg
    31501 atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt
    31561 gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag
    31621 ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga
    31681 ggattgaata attatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg
    31741 attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg
    31801 aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat
    31861 aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt
    31921 taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac
    31981 tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag
    32041 gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga atattttttc
    32101 ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt
    32161 gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca
    32221 tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa
    32281 tttttttgtt cctatttatt taac
//
LOCUS       DANJMA010000028        32216 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000028 DANJMA010000000
VERSION     DANJMA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(99..1229)
                     /locus_tag="PWU31_001546"
     CDS             complement(99..1229)
                     /locus_tag="PWU31_001546"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL1084095.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PWU31_001547"
     CDS             complement(1878..3080)
                     /locus_tag="PWU31_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1084096.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PWU31_001548"
     CDS             complement(3140..3343)
                     /locus_tag="PWU31_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL1084097.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PWU31_001549"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PWU31_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PWU31_001550"
     CDS             4880..5692
                     /locus_tag="PWU31_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084098.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="PWU31_001551"
     CDS             complement(6716..6868)
                     /locus_tag="PWU31_001551"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL1084099.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="PWU31_001552"
     CDS             complement(7059..7937)
                     /locus_tag="PWU31_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1084100.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="PWU31_001553"
     CDS             complement(7976..9094)
                     /locus_tag="PWU31_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL1084101.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="PWU31_001554"
     CDS             complement(9091..11190)
                     /locus_tag="PWU31_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL1084102.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="PWU31_001555"
     CDS             complement(11241..12062)
                     /locus_tag="PWU31_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1084103.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="PWU31_001556"
     CDS             complement(12059..12868)
                     /locus_tag="PWU31_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084104.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="PWU31_001557"
     CDS             complement(12889..13383)
                     /locus_tag="PWU31_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084105.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="PWU31_001558"
     CDS             complement(13395..13883)
                     /locus_tag="PWU31_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084106.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="PWU31_001559"
     CDS             complement(13960..16656)
                     /locus_tag="PWU31_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1084107.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="PWU31_001560"
     CDS             complement(17084..18157)
                     /locus_tag="PWU31_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1084108.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="PWU31_001561"
     CDS             complement(18154..18981)
                     /locus_tag="PWU31_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084109.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="PWU31_001562"
     CDS             complement(18982..19788)
                     /locus_tag="PWU31_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084110.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="PWU31_001563"
     CDS             complement(19800..20885)
                     /locus_tag="PWU31_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084111.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="PWU31_001564"
     CDS             complement(20902..21444)
                     /locus_tag="PWU31_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084112.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="PWU31_001565"
     CDS             21702..22691
                     /locus_tag="PWU31_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL1084113.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="PWU31_001566"
     CDS             22688..23425
                     /locus_tag="PWU31_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL1084114.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="PWU31_001567"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="PWU31_001567"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL1084115.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="PWU31_001568"
     CDS             23989..24621
                     /locus_tag="PWU31_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1084116.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="PWU31_001569"
     CDS             24706..25353
                     /locus_tag="PWU31_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1084117.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="PWU31_001570"
     CDS             25358..26341
                     /locus_tag="PWU31_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL1084118.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="PWU31_001571"
     CDS             complement(26744..28258)
                     /locus_tag="PWU31_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL1084119.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="PWU31_001572"
     CDS             28396..29202
                     /locus_tag="PWU31_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084120.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="PWU31_001573"
     CDS             complement(29377..29946)
                     /locus_tag="PWU31_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1084121.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="PWU31_001574"
     CDS             complement(30029..32089)
                     /locus_tag="PWU31_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL1084122.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
BASE COUNT         9643 a         6751 c         5486 g        10336 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtcca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgggccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgt
//
LOCUS       DANJMA010000029        30956 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000029 DANJMA010000000
VERSION     DANJMA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(10..2082)
                     /locus_tag="PWU31_001575"
     CDS             complement(10..2082)
                     /locus_tag="PWU31_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1084123.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERIPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2015..2392)
                     /locus_tag="PWU31_001576"
     CDS             complement(2015..2392)
                     /locus_tag="PWU31_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL1084124.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2521..3099)
                     /locus_tag="PWU31_001577"
     CDS             complement(2521..3099)
                     /locus_tag="PWU31_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1084125.1"
                     /translation="MRGNMKMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYL
                     SLRFLGYFTKESTIDPEDSYFWMINDFIATLP"
     gene            3231..4523
                     /locus_tag="PWU31_001578"
     CDS             3231..4523
                     /locus_tag="PWU31_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1084126.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITVIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATDNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            complement(4583..5701)
                     /locus_tag="PWU31_001579"
     CDS             complement(4583..5701)
                     /locus_tag="PWU31_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL1084127.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5715..7136)
                     /gene="arcD"
                     /locus_tag="PWU31_001580"
     CDS             complement(5715..7136)
                     /gene="arcD"
                     /locus_tag="PWU31_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL1084128.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7383..8504)
                     /gene="mnmA"
                     /locus_tag="PWU31_001581"
     CDS             complement(7383..8504)
                     /gene="mnmA"
                     /locus_tag="PWU31_001581"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL1084129.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            8908..9507
                     /locus_tag="PWU31_001582"
     CDS             8908..9507
                     /locus_tag="PWU31_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1084130.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10930)
                     /gene="efmA"
                     /locus_tag="PWU31_001583"
                     /pseudo
     CDS             complement(9657..10930)
                     /gene="efmA"
                     /locus_tag="PWU31_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(11201..11545)
                     /locus_tag="PWU31_001584"
     CDS             complement(11201..11545)
                     /locus_tag="PWU31_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL1084131.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11682..12827)
                     /locus_tag="PWU31_001585"
     CDS             complement(11682..12827)
                     /locus_tag="PWU31_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1084132.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            complement(13007..13981)
                     /locus_tag="PWU31_001586"
     CDS             complement(13007..13981)
                     /locus_tag="PWU31_001586"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL1084133.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLTIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14061..14936)
                     /locus_tag="PWU31_001587"
     CDS             complement(14061..14936)
                     /locus_tag="PWU31_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL1084134.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELTNCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15066..16973
                     /locus_tag="PWU31_001588"
     CDS             15066..16973
                     /locus_tag="PWU31_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL1084135.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAIYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17514..20780)
                     /locus_tag="PWU31_001589"
     CDS             complement(17514..20780)
                     /locus_tag="PWU31_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL1084136.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEGNAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPIRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEQYVEM
                     ASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQTIVLA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWVLR
                     GFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTVKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIDVVVYVMKQRKK
                     "
     gene            complement(21051..21596)
                     /locus_tag="PWU31_001590"
     CDS             complement(21051..21596)
                     /locus_tag="PWU31_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1084137.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21608..22300)
                     /locus_tag="PWU31_001591"
     CDS             complement(21608..22300)
                     /locus_tag="PWU31_001591"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL1084138.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSQDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22319..22603)
                     /locus_tag="PWU31_001592"
     CDS             complement(22319..22603)
                     /locus_tag="PWU31_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084139.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22616..23179)
                     /locus_tag="PWU31_001593"
     CDS             complement(22616..23179)
                     /locus_tag="PWU31_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1084140.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23406..24056
                     /locus_tag="PWU31_001594"
     CDS             23406..24056
                     /locus_tag="PWU31_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL1084141.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            24180..25376
                     /locus_tag="PWU31_001595"
     CDS             24180..25376
                     /locus_tag="PWU31_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL1084142.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25477..26853)
                     /locus_tag="PWU31_001596"
     CDS             complement(25477..26853)
                     /locus_tag="PWU31_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1084143.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSFSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVTAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26890..27756)
                     /locus_tag="PWU31_001597"
     CDS             complement(26890..27756)
                     /locus_tag="PWU31_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL1084144.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27845..28870)
                     /locus_tag="PWU31_001598"
     CDS             complement(27845..28870)
                     /locus_tag="PWU31_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1084145.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28888..29562)
                     /locus_tag="PWU31_001599"
     CDS             complement(28888..29562)
                     /locus_tag="PWU31_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084146.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29743..30639)
                     /locus_tag="PWU31_001600"
     CDS             complement(29743..30639)
                     /locus_tag="PWU31_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1084147.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(30713..30838)
                     /locus_tag="PWU31_001601"
     CDS             complement(30713..30838)
                     /locus_tag="PWU31_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1084148.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         8957 a         6621 c         5531 g         9847 t
ORIGIN      
        1 catatcttat tatttcttca ttgccgcttt tagtgctgct gctaatgggc tttcttctgg
       61 ttcttctgct tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt
      121 cttctgctct tttttatcaa gcattttttc tgtcgttcca tcgtatttac aacggaaata
      181 agcaccgttt ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt
      241 agagactttt ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat
      301 tttgccgtca cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg
      361 ggtaatcgag aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt
      421 tgtttctttt tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc
      481 ttcaaggtct ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa
      541 tactaataat tgcttacctt taggagatac ttgcagtcct tgagggatac gttccatcag
      601 ttcagatttg attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta
      661 tttttccatt tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc
      721 ttttttgatg gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt
      781 ctcagtttca gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa
      841 tcgttcttta ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa
      901 cagtcctaaa aaacgagaga caatcatttg atagattttt tgctcttcat tacttaattt
      961 ttcataacgt gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc
     1021 ttggaatact tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg
     1081 agcaaagtcg ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag
     1141 atatttgctg tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac
     1201 aagacttaat gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc
     1261 tgttaaatca taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac
     1321 agttcctttg cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa
     1381 agcaaattga tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt
     1441 gaaatacgtt tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag
     1501 tgtcggtgtc tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct
     1561 ggtcacattc aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa
     1621 tgcatcatat tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt
     1681 ttgagaagag atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca
     1741 gcgagcaaca agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc
     1801 ttttcgctga gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt
     1861 gattcctagt tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt
     1921 attcagatct tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac
     1981 cttgggtcct tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc
     2041 tttcgcaacg cttggttttt ccgctagaat tagttgtttc ataaaattcc tttccgcttt
     2101 ctactgtgat gggctgatga tttttcaaca acgcaagtcc tttgtattgc tgtaaatcat
     2161 cgtcacactc ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac
     2221 tttttgcatt gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt
     2281 gtcgtgcttc ttcgaaagac tcgaatgttt cttctgcttc aatatcttct tcccagtctt
     2341 caaaaaacca ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc
     2401 tccttctttc agcttttcta gacttatctt agtcactttt caagaaagtt gcaagaattt
     2461 tctcttataa aaggatcatt taatgaacat cttgaaaatg atataaatga atctgattgt
     2521 ttaaggcaaa gtagcgataa agtcattgat catccaaaaa tagctgtcct ctggatcgat
     2581 tgtcgattct tttgtaaagt atcctaaaaa acgcaaggaa agatagccgt ggatcatact
     2641 acataattct ctgcttcggt ggactttttc ttctttcgtt aattgaaaag cctcaaagat
     2701 ctgcaaaatg atctggttcg tttcatggat tccctctaat aaaactgctt cattggtgtg
     2761 gggcacactg atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt
     2821 ttctgcatag atctgcagtg cttcctgacc actttttccc acaagagccc gacgcagctg
     2881 gtcattaagt tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt
     2941 gaaatggtta tacagcgagg gagccttgat atccaagcgt tttgctaaat gctgaaaggt
     3001 tatttgctga acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg
     3061 agacagattt ctttttttca ttttcatgtt tcctctcata aaataaatcc ttcactcttt
     3121 cagtttacca cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta
     3181 gtttataaca aaaagatgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa
     3241 gaataaagga aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat
     3301 ggtttggaac atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt
     3361 acatagatac gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt
     3421 tttcatctac tttcttggtg actacgctga tctctccttg gtggggaaaa ttagcccacc
     3481 aaaaaggaag aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt
     3541 tgatgggttg cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt
     3601 tttctggtta tatcagtaat gccgctgccc ttgtcgcaac tgggacgcca aaagaaaaaa
     3661 gtggtcaagt tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc
     3721 ttcttggcgg gatctctgct tcagcgtttg gctatcgccc tacctttttc attacagtga
     3781 tcatcttatt tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag
     3841 tgaaaaaaga aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg
     3901 tcgtcatcgg tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc
     3961 caatcatcag tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca
     4021 gcggaatcat cgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc
     4081 gcttaggtga caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc
     4141 tggtttatat cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt
     4201 tgatgggtat ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt
     4261 ctcctcatca agctgctgga cggatcttca gttataacca atctttccaa gcaactgata
     4321 acgtagtagg gcctatgatc ggctccagtg tttctagtgc atttggttat cgtggcgtct
     4381 ttctttcgac ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag
     4441 agatccatca gcaagaaact gcagaagaaa aagcttcagg agtctccata tcttcagggc
     4501 actctccttc tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag
     4561 actgtccttg cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata
     4621 atcccttttc caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat
     4681 caaaggcact tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg
     4741 tctggtcatc ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga
     4801 caagttcaac tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa
     4861 tgatcttttt gtttcgctcc aatatttttt ctttattctt caatgcatgt acagcaagat
     4921 catctgacag gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg
     4981 ccaactcagg tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg
     5041 atagggaatt ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt
     5101 tatccgttaa aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa
     5161 tttctgcgat tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac
     5221 tattcaaaca gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa
     5281 actgccaatc ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg
     5341 ggatgtcata taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca
     5401 ctaacgcgta tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt
     5461 ccggatcgac ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga
     5521 tccagccata gtccaatggt tcgtttttgc gtttttcgaa aaattcactt acgctagtcc
     5581 catctaagcc aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat
     5641 ataccgcttt cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca
     5701 tgtttctttc ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt
     5761 gcggccacac aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt
     5821 tcacgacaag cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg
     5881 acataagaaa atcctgctaa aatgatgccc atcaattcaa aagctacagc aatcccaccg
     5941 atcactactt gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca
     6001 agtagataac aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa
     6061 tcagtaacta agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa
     6121 taagttgggg catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga
     6181 gccatcaaca tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc
     6241 gaaataatca agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa
     6301 atatttgcca tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat
     6361 ggcaagatcg aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct
     6421 tgctgggctt ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct
     6481 acaaagaccc acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc
     6541 tgccctgttc cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata
     6601 ttcactttaa atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag
     6661 atcgtcacga ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc
     6721 agaccccaaa taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat
     6781 atcgcgaaaa attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc
     6841 cacgcagaca accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct
     6901 tccgcgtaac cgaaaatacc accatctaga tctggacgtt tttctcctaa atgattcagc
     6961 gataatacca aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct
     7021 ggagctgcag aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa
     7081 ctgacaacca gagcaaccag tccccatttg ctaatacccg ttgatttctt ttccatttcc
     7141 atcaccctca cttataaaat tgaacaaatc acttgcaaaa gatttccttc tattttgcaa
     7201 ctgctcttgc ttaacaaaaa gcaactttat tcatattatc ggatattatg aactttttac
     7261 acaagtattt caagtataaa atgaataaaa attaatttta ttgcaaaaaa aacagctatg
     7321 ctatccaaaa gaaaaggtgt ttcttttgtt agccatagct gttttgattc atttaatatc
     7381 gcttaaacgt attgtaaaac ttttgtttct tgatatgcgt ggtcgatcaa accaccaccc
     7441 agacattcca tttcatcata aaatacaact gcttgtccag gagtgattgc acgtacaggt
     7501 tcgtcgaata cgacttctgc atgattatca tccaataagc gaactgtcac aggaacgtct
     7561 tgctgacgat aacggaattt cgctgtacac ttgaattctt ttggcattgg ttcattcgtc
     7621 gtaaagtgga tttcacttgc atccaagctt gtggcataca atgcaggatg atggaaccct
     7681 tgccccacat ataatgtgtt tgtcgctaga tcttttccta caacaaacca gggttcttgc
     7741 gaatctcctc cgccaccgat ccctagtcct tgacgttgac caatcgtata atacatcaag
     7801 ccagcatgtt gccctttgac ttcaccatca agagtgacca tatttccttt ttttgctggc
     7861 agatagttgc tcaaaaattg tttgaagttc ttttcaccaa taaaacagat tcccgttgaa
     7921 tcttttttct tagcagtcgc aaggcctgcg cgttcagcaa ttgcccgtac ttctgatttt
     7981 tccatgccgc ctagagggaa catcgttttt gaaagctgtt cttgtgataa ttggctaagg
     8041 aagtaagttt gatctttatt attgtctact ccacgaagca tgtggaccac gccgttttca
     8101 tcgcgagtga cttgcgcata atgtccagta gctacataat cggcgccaag atccattgca
     8161 taatccaaga atgctttgaa tttgatttct ttgttgcaca tcacgtctgg gttaggtgta
     8221 cgtcctgcgc ggtattcggc taggaaatat tcaaatacac gatcccaata ttctttttca
     8281 aaattcacag aataataagg aatcccgatt tgagcagcta ctttcgctac atccttataa
     8341 tcttccgtgg ccgtgcatac accgttttcg tccgtgtcat cccaattttt catgaatatt
     8401 ccgatcacgt cgtatccttg ttcctttaac aaaagtgctg ttacagatga gtcgacaccg
     8461 ccgctcatcc ctacgacaac gcgtgttttg ctgttgtctg tcatgatatc accatcattt
     8521 caattagatt ctgaaacatc tacgagttga aggtcgcaac ttttcagtat tttacattat
     8581 acgtcatatt tctgaaaatt tcatccctta attatcaagt cgtaaaaata ctgttctact
     8641 ggacctattc agtacacaaa catgatagtc aaccaaaaaa tcactcatct tttgagcatt
     8701 cgatataaaa aattggcata ctcataaaaa gggccagcct ttcggcaata aggaataatt
     8761 ttataaattg tgaaaaacta tttttcaaaa ttgcttctta tcgcttaaag ctacacgtct
     8821 catttacaac cttttctttt tagatgttat gagttgctca gataagtgat tttgtttact
     8881 ctaagaaaaa agggaggcaa aagatacatg aattacttta ccagtgatat gcactttttc
     8941 catgaacgct tattaggaaa aaacgacttt gctccgcggc tctttgcaaa tgtggatgaa
     9001 atgaaccaac agttgatcac tagctggaat gcagtaataa aagaaaccga ccatgtttat
     9061 catctaggcg accttgctat gcatccgcaa tatgaaaaag caaatgcaga aattttggaa
     9121 ctggtcactc aactgcaagg aacaatccac ttgatcaaag ggaaccatga cagtcgcgct
     9181 tttttccgtt atttagaaaa aaatgatcct aggcttactg acggaagtcc caaattttat
     9241 ttttatgatg taggtgccat cgttaaattc gaccatcaac aatattactt gactcattat
     9301 ccgatgttgc tcgggcctaa cgacactatc cgcaatcttc atggacatat ccatcattac
     9361 agcgtaccta tcgagaacga tgtcaatgta ggcgtagacg caccggaaag ggaactctta
     9421 aaggaaaaac taccatttgg cactccgtta tctgagcaac aaatcaaaga aatctatgaa
     9481 gcaaagagga agtacgatag gtcgtgaaaa aactgcccta tatcaagtat taaacaaata
     9541 aagataatta ctcatatttt tcatttgagt aggcttattg ccacaggaac attaggatca
     9601 gaaatggagt tgtaacaaga cttctgtcac aactccattc catactattc gtctttttac
     9661 tcatggtgtt cttttaccaa ttttttctgt aattctttat cataatttcg agcagctgac
     9721 gttaaaaata aaacgattcc gcataataaa atcccgattc cagaaaacaa gaacattttt
     9781 tctaccccta aacgatcagc taatggtcct gcaaataata atccaattgg acctgccagg
     9841 ttcatcaaag agttgaagtt ccctaagaca cgacctaaaa cattcgattc ataactctgc
     9901 tggatcattg ccatcagcag tgtattgaaa tatggcgttg cacaacctgc tcctgcattc
     9961 aagataagaa agtacaagaa cccttgacta tcgtttggaa ccaatccgct tgcaccgatc
    10021 gtcaggccca taatgatgta ggctataata ataggtttca ttcggtcttt ccatgttcct
    10081 ataaaactga tcagtgcacc gcctaagagc attccgactg cgtagactac ttccactgct
    10141 cccgcctgtc caacggaacc accaaagtaa tctagcgtca tcaatggata cagactgatt
    10201 gctggcatga acaacagcat cacaaatgca ccattgatcg ttatgtacca taaccctttg
    10261 ttatccatca attgctgcag cccaaatttt gcatctttaa aaatagtaag cggaacttcc
    10321 aatatttcag gagaaacttt tgggatcttc acaaaaatca ataagccgac accaaaaact
    10381 gcccccaata catctaataa aataaggtaa ttgacaggaa cgactgaaaa caaagccgca
    10441 cctagagctg gcgcaatgat gaaattcgct gattggacca ttcctaactg tccattcgtc
    10501 cttgttatgt gagaagatgg aacgattgtc ggcataatcg actgatcgtt ggcatttgga
    10561 atgtttgtgc gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt
    10621 ctgaaatcgt tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga
    10681 caatcaaaag agctttttta tcccagcgat ccactagcgg ttctacaaat gggcttagta
    10741 aaaccatggg aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt
    10801 ctcttgtcaa ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa
    10861 actgtcccga taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt
    10921 cattttccat aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca
    10981 ttatacggga ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt
    11041 aaatttcttt acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa
    11101 atttaaaaat aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa
    11161 taggccgttt caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata
    11221 acccacggct aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt
    11281 ttggatcttt gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca
    11341 atgtttgaat atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg
    11401 tggcatctaa tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat
    11461 cgcgcaatgt atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta
    11521 ccgttgctgt tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt
    11581 ataaaagttt atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc
    11641 gttctctcag acatctgttc atataagaat gacttcacta gttatttttt gacagcacga
    11701 actaattgtt caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata
    11761 cggatggatt cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa
    11821 ggctcgatat tacctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca
    11881 agcttcatca gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg
    11941 agtttattcg ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca
    12001 agaatcaatt gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac
    12061 agcagttcaa ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc
    12121 cgcttttctt cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta
    12181 tataagaaac cgacgccttt tggcccattg attttatgag cagagatact taataaatcg
    12241 attcctaatt cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg
    12301 aaataagctg gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg
    12361 atctcattgt tcccatacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg
    12421 acctcttcga tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc
    12481 tttgtctcta aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt
    12541 agatgtctgc cttctttttg acacgagaac gctgtttcta agatagctgt attatcccct
    12601 tctgttcccc cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg
    12661 atttgtcttg ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa
    12721 ggatttccaa acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg
    12781 ggggtagttg ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta
    12841 ttgctaggca cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct
    12901 agaggacgtt tcatgggcat agaaaaaagg agctatgaca aaagtcttgt cgcagctcct
    12961 tgaagctgct cacttgtgtc acaacttctt tccctctttt ttttgtttat tcgaattctt
    13021 tttcttccag acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt
    13081 ctcccattaa ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg
    13141 tgaaaacaga atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg
    13201 catgatcgga caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt
    13261 ttaatacatt tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga
    13321 tacatgtctt tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg
    13381 catgatctac gatggttaat gtgctgttca aatattctga aagactacgt gcacgctgaa
    13441 caccgctgtt ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt
    13501 aataatgagc aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt
    13561 gtacttgaac cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca
    13621 agttagcgat caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag
    13681 catacccata gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat
    13741 cgatcatgat cagtagttcc atatagtagt cattgacagg tgcattcgtt gattgaacga
    13801 tatacacatg atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa
    13861 attgcttcac gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta
    13921 atggacggtt gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca
    13981 tgtagtcctc cccaaagctt ctctattctt cgatagttta acatattttt ccaagatttt
    14041 ctgttaattt cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga
    14101 atgactttat gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca
    14161 gtttcaaaat aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc
    14221 aaaataccaa tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa
    14281 cctagaagag ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc
    14341 cctgtactaa cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat
    14401 gccttcgtca aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc
    14461 caccccttac gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa
    14521 agatagcctg agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc
    14581 acccagcaat ttgtgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct
    14641 tcttttctgt tgatccatct agataactcg attggctctt cattgatcag cttccatttt
    14701 ttacaagaaa gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct
    14761 gattgatcga taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta
    14821 ctttttcttg actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg
    14881 aaatataaag gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata
    14941 agtgaaggat tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc
    15001 tatttgttaa ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc
    15061 gatgaatgaa caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc
    15121 aaaaaacagc aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg
    15181 acgggatcga ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag
    15241 tgtcttcaga aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc
    15301 gaagaaatga gttgcatgct caaccacttc aacctataat cgaaaaagtt gaatcattgc
    15361 agtctttcga cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc
    15421 catttaatct ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt
    15481 atccgccttc tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg
    15541 cacaactaat aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag
    15601 aaaaaagtca ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc
    15661 ctcatgtaaa aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt
    15721 ttgatacatt tattcaatac acagataaac tggacttgaa aaaacttgtc acgggtttga
    15781 ttgggacaac tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg
    15841 acatcctgac agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac
    15901 gttctcttag tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg
    15961 tattgtctgg aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg
    16021 gacaattcga tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta
    16081 aaaaagacgt agaacaaatg gtgaaaaaaa tgatcgccat ttataaaaaa cgtctagaag
    16141 aaaatgactg gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag
    16201 gtatccaagt aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac
    16261 ctgcagatca gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa
    16321 aagatcgctt cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg
    16381 acacggtcaa tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc
    16441 ttcaagcacc attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag
    16501 ctgttatcgc tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat
    16561 atggcaactt gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac
    16621 aagcaatgat cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat
    16681 taaccgtttc ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa
    16741 aaacggaatc agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta
    16801 caaaagcaaa aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta
    16861 aactccgagc aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca
    16921 cggaacaaga cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg
    16981 tgccagaagt ggtcagcttc aagaaataag aaggaattgg ggaaaattgc ttctcaaatt
    17041 tttgtgacat ttcggcttat tttttgaaga agttacttct ggaataccat gtatcagagg
    17101 ttgtgaaaaa gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac
    17161 atttttgcat aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga
    17221 ggttgtgaaa tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc
    17281 atattttgcc gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga
    17341 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag
    17401 gagaacctca tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc
    17461 tatttttact ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt
    17521 tttcgctgtt tcatgacata aacaactaca tcgattaaca aaattcctcc agctaatgct
    17581 agcaacgggt tattaaattc tcccgttttt gggagtcctt gagtaccatt tttttcactt
    17641 tcttttgctg tcctatccgg atgagaaggt tttccatttg gtgattcatt tcctggattt
    17701 tctctttctg gtttgtcatt tcccgggggc acatcggcag tttctcgcac agtgatcgct
    17761 ttttgctggg taacagattc tgtatgagca gaccattttt ccccatcaaa tatttccgaa
    17821 cgataagtga cttctagcgt atattctcct tttgaaggtg ctgaaaaaat agcggtatga
    17881 ttagaaaatt gagcacttga ctgtgtttca acgatttgga tcttttctgg gaaataacgc
    17941 atatcttcaa agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa
    18001 ggcttcagtt caatgatatt tccttctgca ttctccgcca caccatttgc ttcttgtata
    18061 ataatcggat tattttgttc atttggtttt gtctgatttg gttcttgtac gattgcatac
    18121 tctggatatt ccgaagacgg cgaggtttct ccgtcaaaaa tagcacctac agataatgga
    18181 agaccatcca ctctttcctt tactgtttga cgattccccg gtttaatctg catcagtcca
    18241 tgaacatgga tacttccatt ttcatagcgc gtaacaggct gctgaccttc tttcgaacat
    18301 gtatttagag actcgatcca atcttttgat acggtatgtc caagagtatt ttctgattgt
    18361 ttctctcgtt cattccaata gtagttcaac gagtggtaga tttgatcttc gttttgattc
    18421 aatgggcgga tctgctctct catctcaaga ttctctgttc ggaaagataa gataccagaa
    18481 gcatggtagt ctgtgtttcc tgctgaagag gtataaagag ccacattata tgaaccgtta
    18541 ttaaaacttg tactgctatt gacgataata tctgttccgc tattggaatc gattcctttc
    18601 gcaagatttt cgaaagacaa actattgatc aattcatgag ggcccgttaa agaagaaccg
    18661 cccattttga atccattacc gttaccttct ccctcaattc ccggtaccca gccgtttcga
    18721 taagcaactg agtttttgat gattactttt ccgatcgaac ctgtttcatt tttagcaaaa
    18781 aggtcccaac cgtcatctgc attatgatac gcgatacaac cgtcaaatac atttccttct
    18841 cctattgtca gtttggccgc aaatccatcg gcatcttcaa atcctggatc ggcattttca
    18901 aaagatgtac agttcttgac taagttatgc gctggccatg cttcgaaagg atcatttgag
    18961 ctaccactga tttggattcc tgtatttcca ttccgataag catggatctc ttcaatcaaa
    19021 ttatggtttc cagaaatctg caatcctttt tgcatagcgg cactatttgt aacgtcaaac
    19081 ccacgtaaaa cccagtaatg actaaacagt gtcatcccat ttccagttcc tttaaaatca
    19141 agtacaggtc gcttgttttc tggatttttc tctgcaatca attgaatcgg gttttctctt
    19201 gtcccgttaa ctccttttgg aatcgtcaat cctctttcaa aggaataagt tccaggagcc
    19261 aggacgattg tctgcccagc atatgcgtat ctcaatgctt gcgtcagaga taacggattt
    19321 tgtctgcttc cgtttcctgt atctgaccct tccggtgtta cataaactgt cgaatagtta
    19381 ggtttacggt agtcaactgt atgcagaatc tcttttgttt cataactagc catctctacg
    19441 tattgttctg gcggatagtt tttatctggt gtaaaactga aagtaaattc gttcgttcct
    19501 tccatcagtt ttgtctgata atcaaattcc cgatttgcag tgattgccaa gtcttttgcg
    19561 ttctcaagta cctctcctgt tgcttgattt ttgattgtta ataatccgtc agcatttgct
    19621 ctaaaggaca tagtatggtc ttggtttcct gaatgattac tagaggtcac agtgtaaatc
    19681 ggatcaatca agcgaatggg cctttcttgt ccaggaggat ctgtcgcagg atctgagtaa
    19741 gccacttcga ttccttctac cttgatcgtc atattccttg ctgcagcaaa tcctgcataa
    19801 ataaccgatt catctgaagc gtataatttt tcccagtcat acatgatgtc agaagccatt
    19861 tcttctcctg tttcaggatc gtaataatat gcctctaaac cagtattcgt ttttttcatc
    19921 tctacgtcca gtttagtaat cgagttcgtg gccggaaaca gattcccatg accgtttccg
    19981 aatatattat aagaacctgt ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt
    20041 gtttccactg agacactccc aggagctggc gcagcttgcg gatcttcaga tgatattcct
    20101 gtgattgccc gtgtccctaa tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc
    20161 acttcttgtc tatccgagtc ccagacatat tcgatccgac tgcctagtat ggcaaatgaa
    20221 tttgaataaa atgcctgccc aaacaaatga ttttttggta ctgcgtctct taccattaaa
    20281 gcaaatcctt cttgggcatt cgtataggtc caagattcta tttcgatcgt cgcccgcatc
    20341 ctaaagttga tgtctttggg gattgcttga taataatagg tcattccatc tgatccgctt
    20401 gactggaatt ttccgccgtt attcgtggaa gagagccgca cgccgtcttc taatggttca
    20461 atggcatacc tactagcact tgtgctttct ccgaaaacaa cccctttcca ctcttcattt
    20521 gcttcttcag attgcttttt tgtcttttca ttttctgatt ctttcatcat aggatcattt
    20581 ccttcttccg tatccatatc ttcactagtt cttggtactt cctccattgt tgacatccct
    20641 tcaatgttca cgtgtgcatt tccttcgttc acagactgtt gttcgttagc agcaacttca
    20701 acgacagaaa ttaaaaccgg ggtactgagc ataaggagat tcatcattag cgcactgtat
    20761 tttatacgtt tttttaacaa gtgtattcct cccgcaacaa attagaaagc gtttacttaa
    20821 agtattacct aatttatttt tactgtcaat aaaaaacaaa attataataa gtgtatttta
    20881 taaaaagaaa aaatataact taagttgttg ataataaaga gatttattaa cgtaaaaaga
    20941 ataagatttt tcaaaataac gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc
    21001 tcttctttta ttattttgtg ctttttattt tatcaaatta ctttgttttt ttataaaatt
    21061 tctgcgccta gtgtggattc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa
    21121 cttgccacgg catctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct
    21181 gtatgcaaca cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt
    21241 tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc
    21301 caataaacat tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat
    21361 tcccgtccac ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag
    21421 gaatcttttg gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac
    21481 ttctttgtat gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc
    21541 tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta
    21601 gtcctcctta tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg
    21661 cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg
    21721 cacgtacgat caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt
    21781 ccatttcgca agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatcttgcg
    21841 agtcgacaaa tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt
    21901 ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc
    21961 ctggtaattg tcctggtttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt
    22021 tgtctgatat aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg
    22081 tattgatcac catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta
    22141 agacttttcc tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa
    22201 taaataaagc gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt
    22261 ttacttcttc ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct
    22321 atataaatgc gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt
    22381 tcatcaaaaa agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga
    22441 ttggtttatt ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct
    22501 gataagctgt ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc
    22561 gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact
    22621 ttcctttggt caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca
    22681 actgatcttt catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt
    22741 cgtcttcatc ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat
    22801 gaaggacttc atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact
    22861 tcgcacgata gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc
    22921 cttctcctgg atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc
    22981 gaaattgttt gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccaacaccgc
    23041 caggatgaaa taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta
    23101 cagcaacatc aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa
    23161 attgctcatt gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttc
    23221 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
    23281 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
    23341 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
    23401 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
    23461 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggacttt
    23521 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact aacgaacttg
    23581 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    23641 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    23701 ttaaagcaat cgatctccgc catgatccag tcaaagcagc aaaagcttta ttcaaagaat
    23761 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    23821 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    23881 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    23941 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    24001 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    24061 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    24121 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    24181 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    24241 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    24301 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    24361 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    24421 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    24481 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    24541 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    24601 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    24661 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    24721 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    24781 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    24841 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    24901 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    24961 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    25021 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    25081 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    25141 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    25201 aacgtggaat cgtggatatc gagacccttc aaactactca aaatgatttg atcgaaacaa
    25261 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    25321 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    25381 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    25441 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    25501 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    25561 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg aaatgattgg
    25621 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    25681 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    25741 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    25801 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    25861 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagattgt
    25921 taccgtcagt agcgttaaaa tggcccctac agcggtcacc gcgcccactg tcccatgtcc
    25981 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    26041 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    26101 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    26161 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    26221 aaaaggcatg aatggctgcc aattatcagg tttaacgaaa aatattccta caatgataaa
    26281 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    26341 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    26401 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    26461 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    26521 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    26581 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    26641 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaaatgaca gtgctggacc
    26701 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    26761 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    26821 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    26881 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    26941 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    27001 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    27061 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    27121 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    27181 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    27241 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    27301 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    27361 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    27421 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    27481 agtagacaat ggtaacccta cagaaacacc catgtacttc attccatgga actgtccatt
    27541 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    27601 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    27661 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    27721 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    27781 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    27841 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    27901 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    27961 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    28021 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    28081 ctgaaaggct gactccttgt gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    28141 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    28201 cggtcaaaac agtatgatcc tcaccaacaa tctcgtagcg aatatttttt tggataaaat
    28261 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagcctatat tcttggatga
    28321 ggtagtcccg cgaaaaactg tctagtcttg cataatacaa aacttgttct acgtattcat
    28381 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    28441 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    28501 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    28561 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtt cgtccaccgc
    28621 tcaaatagtt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    28681 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    28741 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    28801 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    28861 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    28921 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    28981 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    29041 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    29101 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    29161 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    29221 tgcattgatt tttgcaatca gtacatcgat ttggaaaggt tttgtcacaa aatcatctgc
    29281 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    29341 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    29401 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    29461 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    29521 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    29581 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    29641 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc ttttgtcttt
    29701 tcaatacagc ttaaatttat tatgttattc ttaacaattg gtctatgatc tgttattttt
    29761 ccttagttgt ttttgtcttt taaatttcca ataacaccaa taaccgaaaa gcgttagcac
    29821 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    29881 tatctttgaa gagacttctt ctgtaaaggg aactcgcctt cctgtcacta gtaatcggtg
    29941 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctgat
    30001 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    30061 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    30121 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    30181 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    30241 cgtttcatca aataaaggca agctaacatt gattttcgga atatagattg ctccgatcat
    30301 atgttgttcg tagtattctt ttcctggatt tcggacattt ctaaccgctt gctcaaaagg
    30361 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    30421 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    30481 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    30541 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    30601 gatttgggtt tgaggtctat tacctgttct ctttttcatt ttcttcacct tcttatttaa
    30661 aaaaactgca gcaatcggtt ttataaaggt tgctgcagct tatttttgtt gcttatactt
    30721 cagacttcat tttaaattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    30781 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    30841 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    30901 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataa
//
LOCUS       DANJMA010000030        27641 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000030 DANJMA010000000
VERSION     DANJMA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(18..293)
                     /locus_tag="PWU31_001602"
     CDS             complement(18..293)
                     /locus_tag="PWU31_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084149.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFRKQMKKLIEKEFPRSHKLYVNQQF"
     gene            587..1402
                     /gene="thiD"
                     /locus_tag="PWU31_001603"
     CDS             587..1402
                     /gene="thiD"
                     /locus_tag="PWU31_001603"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL1084150.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFKLIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1607..3058)
                     /gene="cls"
                     /locus_tag="PWU31_001604"
     CDS             complement(1607..3058)
                     /gene="cls"
                     /locus_tag="PWU31_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL1084151.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3400..6066
                     /locus_tag="PWU31_001605"
     CDS             3400..6066
                     /locus_tag="PWU31_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1084152.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6430..7182
                     /locus_tag="PWU31_001606"
     CDS             6430..7182
                     /locus_tag="PWU31_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1084153.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7204..8358)
                     /locus_tag="PWU31_001607"
     CDS             complement(7204..8358)
                     /locus_tag="PWU31_001607"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL1084154.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8543..10984
                     /locus_tag="PWU31_001608"
     CDS             8543..10984
                     /locus_tag="PWU31_001608"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL1084155.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11314..12018)
                     /locus_tag="PWU31_001609"
     CDS             complement(11314..12018)
                     /locus_tag="PWU31_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084156.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12205..12450)
                     /locus_tag="PWU31_001610"
     CDS             complement(12205..12450)
                     /locus_tag="PWU31_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084157.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12467..13318)
                     /locus_tag="PWU31_001611"
     CDS             complement(12467..13318)
                     /locus_tag="PWU31_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1084158.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13483..14487
                     /locus_tag="PWU31_001612"
     CDS             13483..14487
                     /locus_tag="PWU31_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL1084159.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14728..15084)
                     /locus_tag="PWU31_001613"
     CDS             complement(14728..15084)
                     /locus_tag="PWU31_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1084160.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15452..16639
                     /gene="tuf"
                     /locus_tag="PWU31_001614"
     CDS             15452..16639
                     /gene="tuf"
                     /locus_tag="PWU31_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1084161.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16827..19418)
                     /gene="mprF"
                     /locus_tag="PWU31_001615"
     CDS             complement(16827..19418)
                     /gene="mprF"
                     /locus_tag="PWU31_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL1084162.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19444..20205)
                     /locus_tag="PWU31_001616"
     CDS             complement(19444..20205)
                     /locus_tag="PWU31_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084163.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20372..21004
                     /locus_tag="PWU31_001617"
     CDS             20372..21004
                     /locus_tag="PWU31_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL1084164.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21063..21923
                     /locus_tag="PWU31_001618"
     CDS             21063..21923
                     /locus_tag="PWU31_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL1084165.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22108..24423
                     /locus_tag="PWU31_001619"
     CDS             22108..24423
                     /locus_tag="PWU31_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1084166.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24605..26446
                     /locus_tag="PWU31_001620"
     CDS             24605..26446
                     /locus_tag="PWU31_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL1084167.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26470..27579
                     /gene="rpoD"
                     /locus_tag="PWU31_001621"
     CDS             26470..27579
                     /gene="rpoD"
                     /locus_tag="PWU31_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL1084168.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8944 a         4887 c         5762 g         8048 t
ORIGIN      
        1 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
       61 tctttttcaa tcagtttttt catctgttta cgaaattctt ttttttcagt aaacataaag
      121 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      181 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      241 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      301 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      361 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      421 acaaatgaca actattttct gatttattac aaatgttttt tcaaataaat ttgattacca
      481 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      541 acagcattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      601 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      661 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      721 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
      781 atttaaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
      841 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
      901 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
      961 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1021 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1081 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aagatgcgca
     1141 gcaagaaaca gtcaatgact acgtattgct cgaatcagga aaatcatttt ggttaaatgc
     1201 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1261 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1321 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggaccca tcaatcattg
     1381 ggcagctggt gaattagagt aaaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1441 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1501 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1561 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1621 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1681 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1741 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1801 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1861 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1921 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1981 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     2041 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     2101 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     2161 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     2221 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     2281 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2341 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2401 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt gaggtaacaa
     2461 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtaccat
     2521 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2581 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2641 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2701 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2761 cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2821 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2881 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2941 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     3001 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     3061 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     3121 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     3181 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     3241 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3301 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt tttttttcag
     3361 acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa ggaacaagca
     3421 caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca gagctcaacc
     3481 gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt gaaccagcta
     3541 aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa agatgcaatg
     3601 gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta catcgaattc
     3661 ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca aacaaaaaaa
     3721 gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg agtgattcgt
     3781 gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat cgtgttgtta
     3841 gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc attaaaagta
     3901 gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac ggatatcaca
     3961 agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat cgtaacttac
     4021 ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg acaagttgct
     4081 ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct ggataaattc
     4141 agtaaaaaac taagtctggc tattttggca ttgtctttat tgattttagg tatccaactg
     4201 tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt cagcgcagtg
     4261 atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc tatcgtaaca
     4321 atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat tcggaagctt
     4381 ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac tggtacattg
     4441 acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac tggggatttc
     4501 acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat cagtgtttta
     4561 gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac ggaagtagcg
     4621 tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa caactaccca
     4681 agacaagcag aacttccttt cgattctgat cgaaaattaa tgtctactgg acacacgatc
     4741 gataacgaaa attacttgtt tgtcaaaggc ggaccagata tcgtcttcca acgcagtaaa
     4801 aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa aaaattccaa
     4861 gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc ttaccgtcca
     4921 cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt tggtttgctt
     4981 gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc aagaagtgca
     5041 ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc tatcgcaaaa
     5101 caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact agatgaattg
     5161 acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg tgtttcacct
     5221 gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc agctatgaca
     5281 ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat tgctatggga
     5341 acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga taactttgct
     5401 tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa gaaagctatt
     5461 tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc cttagttgct
     5521 gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt caatgattca
     5581 ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa ccgcccgcca
     5641 agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac gtatcgtggt
     5701 gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca ttcacctgaa
     5761 ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca aacattccca
     5821 gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag aagtgtctta
     5881 ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg actgaaagaa
     5941 gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat gggaatcgca
     6001 cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt acaaggaaat
     6061 aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca caaggtgcaa
     6121 tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga agagttggca
     6181 ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat actgagggtc
     6241 atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg gtcacagtcc
     6301 ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa aagagataga
     6361 atagataaaa gaggagaata atttattccg aaaagtgaaa acatataaca aaaaattaga
     6421 ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta tgaaatccag
     6481 caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac ggagaaagtc
     6541 ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct ttccttagca
     6601 aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga aaatgcaaaa
     6661 aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc gttagaagaa
     6721 tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc agcattggta
     6781 catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt gataatcagg
     6841 gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg aatgatagaa
     6901 gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt aattgatgaa
     6961 ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat tactgaagaa
     7021 ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag gtacattcgc
     7081 aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta tagactgctc
     7141 ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct ttttatgatt
     7201 ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa aaggtacatc
     7261 tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa tcgtttcata
     7321 ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt gtgcgaagag
     7381 atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg caccagaacc
     7441 gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt ccagtagaga
     7501 agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc tgtaacggat
     7561 gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt ggtctaatac
     7621 gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa tcgcctgata
     7681 atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt ggaatgattc
     7741 gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat aatctggacg
     7801 ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg ataaaatacg
     7861 cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt caccaccgct
     7921 tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt ctggatgatt
     7981 tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag cctcttttac
     8041 ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta ccgcactcgc
     8101 ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta gctcacggtc
     8161 tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt cttcatttgc
     8221 acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag gatcatcccc
     8281 acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa aggaaagacg
     8341 atcaatccct attttcattt caacacctct ttacttatga taaaaaactt actttactaa
     8401 ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat tgttaaaatt
     8461 taatcttaga aaaaaacaac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     8521 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     8581 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     8641 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     8701 cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     8761 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     8821 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     8881 cggaacagag agtatgtctc aagcacctat gctgaaaccg tatcagtcag aaacaaatga
     8941 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     9001 acatatggga ttaaccgcag agaaggttgc aacacaattt tctgtgagca gagaagaaca
     9061 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     9121 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     9181 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     9241 aggaactgta acagcaggaa atgcttcgcc attgaatgac ggtgcctctg tggtgatcct
     9301 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     9361 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     9421 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     9481 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     9541 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     9601 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     9661 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     9721 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     9781 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     9841 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatgggcat
     9901 cgcgcagaat tttcaaatca atggtaagaa aaaatggatt ccgatggcaa cagaggaacc
     9961 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
    10021 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
    10081 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
    10141 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
    10201 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
    10261 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
    10321 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
    10381 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
    10441 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
    10501 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
    10561 tagcgcttcg attcacgcct acgcggcaag aaatggatta taccaaggat taacagactg
    10621 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
    10681 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
    10741 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
    10801 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
    10861 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
    10921 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
    10981 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
    11041 aatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
    11101 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
    11161 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
    11221 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
    11281 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
    11341 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
    11401 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
    11461 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
    11521 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
    11581 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
    11641 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
    11701 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
    11761 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
    11821 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
    11881 gggatcaagc atgacttgta acggcaaacc atagcgtcgc aagctctctt cttctagaga
    11941 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
    12001 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
    12061 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
    12121 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
    12181 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
    12241 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
    12301 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
    12361 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
    12421 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
    12481 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
    12541 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
    12601 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
    12661 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
    12721 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
    12781 gctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
    12841 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
    12901 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
    12961 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
    13021 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
    13081 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
    13141 cgacactttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
    13201 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
    13261 ctactaccgg gatttcgtaa ccatttaata gacgataagt gcttgttaat gaattcatac
    13321 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
    13381 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
    13441 tttgattttt ttccgtaaac taagacagaa gggaatgaga aaatggatat ctatttaaaa
    13501 aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc acaaaaagag
    13561 ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca aaaaatctct
    13621 tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga atcacttaaa
    13681 atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc
    13741 taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgttttgta tgaattagat
    13801 acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac tcactttgtt
    13861 gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat tctaggggcg
    13921 aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac ctacgaatta
    13981 attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg aagagtgatc
    14041 gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa agtagccaag
    14101 catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt gaaagaagct
    14161 gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc
    14221 ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga gcaaggattt
    14281 gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa atttggaaaa
    14341 caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac
    14401 ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat gatcaaaaat
    14461 gtggatgaag tattgaatcg tttataaaaa tgactagaac attaggtgtt caagtaccag
    14521 tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa
    14581 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
    14641 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
    14701 tatctaataa aattttaaga ccatttacta attatcgatt ttttctgccg catttttagg
    14761 cacttcagaa gcctttattt cttcccagct taatacacct ttacgaggat tgactttgag
    14821 tttaagatac gcattcatct ttaatggatg ttctctttct tcgcgaagcg tcacctgttt
    14881 tttttcacca tctttattat aagccgcttg cttgtaggta taagtcgtga atttctctcc
    14941 gttatctgct tgggtagctg acatttcgcc attcgtcgtg atttttgtat aataagcttc
    15001 gccgccgtaa aaataatgat aagctgcaaa acttccgccg agaaatatgc ctaaaacgac
    15061 aagtacgcca attaattttt tcatgtactt cacctctttc cttattatta acgaaataga
    15121 agatttcgtc tattgaaagt tgttggcaac agctgaattt taaacttttc ctcattttgt
    15181 tcttattgct tcaaaagaaa gttaaggact atcttgaagt ttttctcaaa aaacacattt
    15241 ttttatttat aaaaggtact tttttcgcgt ataagacaag cgagtgctct tagttgactt
    15301 tgaccaatat tgacgtataa taagcttagt tcgaatctta caaaaagtaa gtcggacgta
    15361 ttctttttga tttagtcaaa aaaagactat aataaaaagg tgagagctga aatttttcag
    15421 tgatctatat tatttaggag gtttgtaaat tatggcaaaa gaacattatg acagaagtaa
    15481 accacacgtt aacattggta cgattggaca cgttgaccat gggaaaacta ctttaactgc
    15541 tgcgatcact acagtattag gcaaaaaagg cctagcaaat cctcaagact atgctagtat
    15601 tgatgcagct ccagaagaac gtgaacgtgg gattacgatc aataccgcac acgtagaata
    15661 tgaaacagaa aaacgccact acgcgcatat tgatgcacca ggacacgcgg actatgtgaa
    15721 aaacatgatt acaggggcag cacaaatgga tggtgcaatc ttagttgttt ctgcaactga
    15781 cggtccgatg cctcaaacac gtgaacacat tttattgtca cgccaagttg gtgtaaaata
    15841 cctgattgtt ttcttgaaca aagttgattt agtcgatgat gaagaattga tcgatttggt
    15901 agaaatggaa gttcgcgagt tattgagcga atatggtttc ccaggcgatg acactcctgt
    15961 gatcaaaggt tccgcattaa aagcattgca aggcgatcca gatgctgaag ctgctattat
    16021 ggaattgatg gatacagtag atgaatatat cccaacacca gaacgtgata cagataaacc
    16081 attactattg ccagtggaag acgtcttctc aattacaggt cgaggaactg ttgcctcagg
    16141 tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta gagatcgtag ggatcaaacc
    16201 tgaaacacaa aaagcagttg taacaggtgt agaaatgttc cgtaaaacgt tagattacgg
    16261 ggaagctggg gataacgtag gcgtgttgtt acgggggatc caacgtgacg atatcgaacg
    16321 tggacaagtg cttgctaaac caggttccat tactccacat acaaaattca aagcagaagt
    16381 gtacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcaaca actatcgtcc
    16441 acaattctac ttccgcacaa ctgatgttac aggaacaatc acattgccag aagatacaga
    16501 aatggtcatg cctggtgaca acgtgacgat tgatgttgac ttgatccatc caatcgctgt
    16561 tgaaaacgga acgacattct caattcgtga aggcggacgg acagtgggtt caggtatcgt
    16621 tacagagatc gaagcataag aaacgcgact cgaatatttt ggtaatgaaa aatgtttaaa
    16681 ataaaagagc ggtccaaaca ggatttctgt ttggaccgct ctttgttctt caattgtata
    16741 agaagagatt gtagaagcat gaactttcgt tttacttcag tctcttttat tcatcatgag
    16801 agtgttcatc tatcggtatg cagtttttaa tactttcttc gtatccattt tttcaaacca
    16861 cttattctac cttgtgtctg ctcaactggc gcattatcaa taatcagcaa agcaatcata
    16921 acatatgcaa tccaactatc tctagaatat agagtataac gtgagaccca gtttgtagcg
    16981 tacttgcttt tatattctct taatccatgg aaagaataaa aatgagaacc aaactcgtaa
    17041 accaagtatg caatcctttc ctgtaaaaaa ctctttctgg aagttccgac atttgaaaga
    17101 ggggccatcc ctaaattgaa ccattggata tgttcttctt tcatataggc aaacaaatgc
    17161 aagaacaaga aatccatact gccagaaggt gcttttttag gatcatatcg catcagatca
    17221 atcgttccta ctttattgtt cgtgtaagta ggcatgatcg ttgcaaaagc gacaatttct
    17281 tcttgggtat ttttgacaac tgcaataggc gctctttgta gataatcttc agagaaaaag
    17341 cctaaagaaa accctttttc ctttcgtgaa cccaaccaat tatcagagat atttttgaaa
    17401 gtactcattt gctcagtaga aaatggtggt tgtaagacat caaatgtaaa tccttctcgt
    17461 tcgatccgat tcaagacagc acgagtgccc ttcatttttt tccctgaagt tgtaaaacta
    17521 ttcaggtcta cataagcttc ttcccccatc ttaatgaaat cgtagccgaa ttcatgcaaa
    17581 atcataacaa tctcttcgct tgtttcgtag aaaactggta aatagcatag tcgatctgtt
    17641 tcttcgataa aagcttcgat tgcttctgga aaatcttctt tcttaccaga aggatcaccc
    17701 attacaatac atttattatt gaagcaagcg aattggagaa gaacagttgg ttcaccgtct
    17761 ttttcatagg caaacatccg cttatctttt aagaagatca attgactatc ggagtttcct
    17821 ccataagttg taaggatctt cagggccttt tcttcattaa aggcttcgcc aatctgtttc
    17881 ttttctcctt gaaggaaatg aacaaataga acgatcatga aactaacagc gatgatggcc
    17941 aacaatcccg aaaaccaaat cttttcagaa ggaaataaga aaaaggaaat aaaatggtga
    18001 cgccgatgag ggaaatccgg cagattgtat accccaataa cgatgtaaag caaggacagt
    18061 gcaccaataa tgattccatc gatcgtacgc cactcccatg aatagatcaa ttgttcccga
    18121 aataattcgt ttttagaagc aagaatgatc agaagcagaa tagacaagaa aaccaccggt
    18181 gtgaaactaa aatcacttaa taaaacataa agtaaagcta gaacaatcaa gaagattgta
    18241 ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc ccataatgat taaaagaaat
    18301 cccaatagta tactaggaaa ttggataata aaatgaaatt tcaatgggtt caaactatga
    18361 agccagcgaa actctgtaaa agcttgcggg atagtggcag aaagtactag catgattcca
    18421 gaaaagtata ataagacaac cacgatttta tgcgcaattt ctgttgctaa ttgtttaggg
    18481 ataccagaat aacgctgatc aaaagtggaa ccgatatttt tgaagaaaaa gacgattcca
    18541 atcaaaaacg gaatgatgta gtagaacagg cgatagagca agatccacac aacgactatt
    18601 tctcttggca caccaatagc ggacaacccg ataatcatca ttacatcgaa actcccaatt
    18661 tctcctggaa tcatagacac gattcctatc actgaagcag caatgaataa cggaattgtt
    18721 tgccacagat caatccggat ttccattaaa taaccaacag aaaggaaact aaacaggaca
    18781 ccggtccatt ccaacaatga aactagcaat agctgtcctt gagttttaag tgaggtacca
    18841 ccaaggaatc ctttcttttt gaaagtagta aataaaaaga cgacaggaaa atatagaccg
    18901 ccgccgatca accatatcca atattgcttc aaaaaaggag caactggcgt gaagactaca
    18961 agtaaaaaag aaagcagact gtaaatagaa agtccagcca tcaaaaacaa gaaaactttc
    19021 gataatgctt ggatcacgtt tctgctctct ttcttttgtc catatagttc agagcgaagc
    19081 cccacactaa caaacccgcc gaaaccggca atattgttga ttgtattgat cagccagctt
    19141 gtctcaaaca agtaacgagg attctgcttt tgttcaagca tccgattcaa aataatatca
    19201 taaccaatca taggtaacac agagacaaga ccaatcaaca gcataagtcc gcttttccat
    19261 acaggtatag tggcaaacgt ttctcctaat tgctggatag atagagtttt accgatggac
    19321 atcaattcac cgacaataat gacaatgaca gaaatcaaaa agattgtttt gaacagtcct
    19381 aggtggtttt tcagccattg tatggtattt tttaacaaat aaatcacctt ctattcctaa
    19441 tagttatgtt tcagaaagaa attgaagaac ttctcgattg aagcgttcag gatcttttct
    19501 agctaattca tgtccttgtt ctttcacaag cacaaaggaa gcttttggta tagttttagc
    19561 tatatacaaa gaatgtttta gcttgattac atcttttttt cccacgatga tcaaagtggg
    19621 ggagttgatt ttttttaaat cattttctgt aagcccgatg tcatgcagca aaagtttgat
    19681 aactaaaaga tttttgcgaa gacttgggcg aaataaagag agtaaccata cccatgcata
    19741 atgaatatta ctgatgactc tggcagaaaa acgaacgcct ttaacaagcg tgtttccaga
    19801 atttaaaatc aatcgatgaa cttttttagg gaagctgctg gcaaatacca gtgctagatt
    19861 tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt ttttccagca acatgatcgt
    19921 attcagatcc tcggccatta actgaaaatt caacatagat gcttcgtttg tcgaacgacc
    19981 atgtcctcgg ctatctacta gatacacggt atagtagcgt tcgagtacag ggacctgttc
    20041 tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa aagagaggga agccttgacc
    20101 gctcttttca tagtaaattt cgctcccatc tggcattttt gcatatttct tttctttttt
    20161 cacataatca cctactgtgt aaagtataac gattatatca gtcattgggc aattgttttt
    20221 actgaaaaac aacgatcatt ttcgtaagat gacaaaaaat acaggaaaat tcattttttc
    20281 ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa gataaagaaa tagtaagata
    20341 aaaaaagatg tcaaaataac taggtgatga aatgaagcga aaacgattat ctattatccg
    20401 tattcttttt tacatgatca ttctgacggt tattggtgcg gtgttgtttt taggaggaag
    20461 aacttatcgt tcggtaaaac gagtggaaag ctatcaaata gaaataacaa cagctgcaaa
    20521 aaattatcat atggaaaact acgagaattt gatcaaggcg ataatattga ccgaatcaaa
    20581 aggaaaaggg acagatctca tgcggagctc ggaaagtctg catggagaag ctgagatcat
    20641 tgataatcca gctgacagta ttgatcaagg tgtcaaatat ttggcggaaa tgatagtaga
    20701 agcaaagaaa caaggatgcg atttatggac agctgtccaa gcatataatt ttgggaaaga
    20761 ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca gtagaattag cagaagcgta
    20821 ttctaaagat gtgctgagcc cacttttagg aaatgaggaa gggaaacaat accgatattg
    20881 gcggatccag tccttgcttt acaatggcgg ctatctctat cataatggag gtaacatgtt
    20941 ttatcctgat attgtcaaaa tgaatgaaaa aataatagag acctatgaaa aagtctttaa
    21001 gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa tgaaagaagg agtgcgcaaa
    21061 tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg gtacacaacc aaagactgga
    21121 tgttacggtt tgtgaaggga atgtttatcg gttcaggatt tatcttgcca ggagtcagcg
    21181 gtggtgcatt agcagctgtc tttggcatgt atgagcggct gatttctttt ttagcccacc
    21241 ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc tgttggtcta ggcggagtga
    21301 caggcatctt tctactttct tttttagtca gttttttatt aggtgcgtat gaaacaacga
    21361 tattatggtt ttttgtcgga tgtatcgctg gaactgttcc tgcgttatgg aaagaagcag
    21421 ggaaaaaagg aagagatact tctgatgtga tcatcatggt tgttactttt gttctggctt
    21481 atttgttttt attatacggc gctcaattat ttgacgggca agtcgatcag aattttggaa
    21541 catggctgat tgccggcgga ttgattggtt tgggtatgat cgtcccagga ttgagccctt
    21601 ctaatttttt ggtgtatatg ggattgtata aagcgatggc tgacggattt aaagaagtac
    21661 gacttgaagt gattgtgccg atcggaattg gtgggattct ttgtgttctt ggattgtcaa
    21721 aaatcatgga ttacattttc tcaaaggcat atagcaagct attccatttc attttaggtg
    21781 tagtttttgc ttcaacgatt atgatcattc ctaccgatta cgcgggattg ggagttgctg
    21841 gggtactagc ttgtcctgtt ctttttatag ccggtacagc actaggttgg tggatgagcc
    21901 gtttggagga acaatataag taaagataga ttgataataa aaacagcaca cgggtgattc
    21961 aaacaaaaag gttggcatct tttttgtaaa atcattcgtg tgtttttcta taaattttat
    22021 aaaaacaagt tgtttttaat cttggaacac gctagactta aacacgtgat tatttttgaa
    22081 agttggcgga aaatggagaa aaagaaaatg aatggactta cacagcaaga ggtagttcag
    22141 aaaatggctt ctggccagca aaatgactac caggaagatg catcaaaaag tacaaaacaa
    22201 atattcagcg ataatatatt gacgttattc aattttctga actttggtat tgggatttgt
    22261 ctgttatttg taggtgcgta ttccaacatg gcgtatttag caattattgt cgtcaatatc
    22321 gttattggaa tttatcaaga aatacatgca agaaatcttg tacggaaatt atcgattgtt
    22381 tcccaatcag aagtactagt gatccgggac ggtaaagaac aatcgatttc tactaaagag
    22441 ctggtattag gagatttggt aaaaatcgga gcaggagaac agataccagc cgatttacag
    22501 gttatatctg gacgggcaga agcaaatgaa gctcttttga caggcgaatc tgatttgatt
    22561 gtcaaagaaa ctggagctga attgctttct ggaagctttt tgacaagcgg ttctgtcatg
    22621 gctgaagtta tccgagtagg tgcagataat tatgctgtcc gcttgactag cgaggcaaag
    22681 actcacaaag ctattcgttc tgaacttgta gattcgattc gcaaagtatc caaattcact
    22741 agtttcgtga ttattccttt ggggatcata ctatttttgg aagcactgtt catccgcgat
    22801 gcttctatca aaatttctgt tattgcatct gcagcagctt tgttaggcat gcttccaaag
    22861 ggtttggtat tgctaataag tattgcatta acgacagggg taaccaaact ggcaaaaaaa
    22921 cgaatccttg ttcaagacat gtattcaatt gaaacgctgg cccatgtcga tacattatgc
    22981 ttggataaaa cagggaccat tactgaaggg aaaatgagta tccagcgggt agaagcacta
    23041 catccgctgt acgagaaagc catcccagaa attatgggga cgtatctggc agaaagcagt
    23101 gataacaaca tcactatgcg ggctatgaga gattattttg agctttctaa tcggtatgat
    23161 gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg gcgcgatgga atttccagaa
    23221 ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg aagaagatcg gcttccggac
    23281 agtctaaaaa cagcccaagc gaatggctat cgcgtactta tgttggggat cgctccaaat
    23341 cagcaattga acgaagaaaa accagcattt gttgagccgg ttgctctgtt tgaaatcgat
    23401 gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc ttcatgagga aggtgtggat
    23461 ctgaaagtga tttctggaga caatcctttt actgtctcca atatcgctag acgtgcagga
    23521 ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta ctgaagaaat ggaagtgcga
    23581 gaagctgctc atcgctatac ggtttttgga cgagtgagtc cgcagcaaaa gaaattgctg
    23641 gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga caggcgacgg tgtgaatgat
    23701 gttttggcgt taagagaagc agattgcagc attgctatgg cagaaggtga tggagcaaca
    23761 cgtcagattg cgaatctggt attgttggac tctgatttta ctacattgcc agaagtcctg
    23821 tttgaaggac gccgagtggt caacaatgtg acgaaagtct caggcatctt ttttatcaaa
    23881 acgatttatt catttatttt atcgattata tgtgccgtta cagcaattgg atttccgttt
    23941 atccctatcc aaatcacgct gattgattta gcgattgaag ggtatccttc cttcttttta
    24001 tcttttgaac aagataaaag aaaaatcact taccgttatt tgcctacagc acttattaat
    24061 gctttaccaa atgccttgct tgtagtagtg aatatcattg cggtgtatct acttggaaaa
    24121 acattcgcat ggggaaatgc tgaaactact actttgatgt attacttgct gattgggatc
    24181 agttgcatgg cagttgtaaa agcttgttat cccttcaacc cattgcgcat cttcctagca
    24241 gtaacgacga tcgtagggat atatgtagct gcaatgctgt tccatggaat tttggaagta
    24301 aacttagggt tgggagagag cgtaggatac ttctttgtat tcatgctcgt aaatattgtt
    24361 cttcgagtat tgtactggaa ggctgatatt caaaatcagt tactgaaaag agtagcaaat
    24421 tgatgcttaa tgagatagaa atgtgttata cttgaaaaag caatgaaata tgatacaatc
    24481 aaacatgtat aattgtgtcc tttaaaatcg cactgatttc agttagtgcg attttttacc
    24541 ggttattctc ctgaaatatc ttctgttaaa cccaaaagat atcaatagag cagggggtga
    24601 aaaaatggct caacggattc ctcaagaggt tatcgaaaca gtacgcagtc aaacgaatat
    24661 cgttgaggtg attggacaat atgtccagtt gaaaaagtct ggtaaaaatt acttggggct
    24721 gtgtcctttt catgaagaac gtacaccgtc tttttctgtt gcagaagaca agcagatttt
    24781 tcattgcttt ggttgtggta aaggcggaaa cgtgtttact tttcttcaag aactagaagg
    24841 tctaagtttt ccagaagcag tggtaaaagt ggcagacttc gaacagatac cgttagaaga
    24901 tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa tctgctacgg ggaaactgat
    24961 tgctgctcat gaaaaagcag cggaaatcta tcatcacatg ctattgcata caaaagctgg
    25021 acagccagct ttagaatatt tgcaaagtcg tgggttgtct ttagaattaa ttgaagaatt
    25081 caacataggt tttgcaccta atgagcgttc ttttcttcag caagtattca agaatgaatc
    25141 aatttcaaat gaattgttag aacgctctgg attgtttgtc acgcatgatg atggccgtat
    25201 gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt aattctcaag gccagacgat
    25261 cggtttttca ggacgctggc ttgcacctga aaaagggcag gaaaaagatc agcccaaata
    25321 cttgaatagt ccagaaacag aactgttcaa taaaagacaa gttttattta atcttgataa
    25381 agcaagaagc gatatccgga aaaacggaga aattttgttg ttcgaagggt ttatggatgt
    25441 gattgctgca tggcaggctg gtatcaaaac tggattagct tctatgggga caagtctaac
    25501 agctcaacag atccaacaaa tggaaaagct gacgaaagaa ttggtctttt gctatgacgg
    25561 agacaacgca ggttttgaag caaccaatcg agggattgaa ttgcttcggc aaaacagtcg
    25621 attagcactt tctgtcgtag tcgttccaga aaagcttgat cctgatgaat atctccgtaa
    25681 gtatggagaa gcatctttcc aagaattagt ggctcatggg cgcgaaacag tctttacttt
    25741 taaatcacgt tatttaaaac aagggaaaaa tttagataat gaaaaagaca agatcgaata
    25801 cctcgaagaa ttactagttg aacttagccg agttgtttca ccaatcgaac aagatatgta
    25861 tctatctcaa ctgtcaacag agtttcagat cggcagagag acgatacaaa aacaactgcg
    25921 cgatcttcga aggacaaatc agcaaaatcg tcaagaacct caggaaagtc aacacactca
    25981 ggttacgaga caagaaactg cacggaaaaa gagacctttg acgcaagtag aaaaggcaga
    26041 gcagattcta ttatatcgtg catttaaaga agtttctgtc cgtaatattt taaaagaacg
    26101 agaatttcag tttattcatg atgcatatgc agaagtttat tttttattcg atgcatatgt
    26161 ctcacaacat tatgaattca accttgcaga gtttttagac tttttgcaag atgaaaattt
    26221 aaggcggttg gtggtggaaa tcgaatattt accggtggca gaagaaagtt cgccacgaga
    26281 aatccaagac ttgctagatg tgatcagcaa atctggctta gctgatgaaa taacattaaa
    26341 aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa cagcttgagc ttgaattgac
    26401 gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc aaatagctac tcaagggggg
    26461 ccttctttta tggcaaatgg aacagataaa aaaacgtatg aagcagcagt agcagctttc
    26521 atcaaagaaa acaaacccaa acgacaagtt gtatatgatg atttatccaa tcagcttgct
    26581 acaccattca ctttgaatgc agatgaaatg gacaaattga tccaaaagat tgaagacgca
    26641 ggtatcagtg tggtggatga aaatggtgat ccatcaattc acagcttgaa gagtgctgag
    26701 aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc caaccggtgt aaaaatcaat
    26761 gacccggttc gtatgtatct gaaagaaatt ggccgtgtct cccttttaac tgcggaagaa
    26821 gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag aagcgaaaca gcgtctggca
    26881 gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt atgtaggacg aggcatgcag
    26941 ttccttgact tgatccaaga aggaaacatg ggactgatga aggcagttga aaaatttgac
    27001 taccgcaaag gatttaagtt ttcaacctat gctacatggt ggattcgtca agccatcact
    27061 cgagcaatcg ctgaccaagc acgtacgatc cgtattccag ttcatatggt tgaaacaatt
    27121 aataaattga tacgtatcca acgtcaattg ctgcaagacc ttggaagaga acctacgcca
    27181 gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag tccgagaaat cttgaaaatc
    27241 gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag aagatgactc tcacttaggt
    27301 gatttcatag aagaccagga agcaacaagt ccagcagaac acgcagctta tgagttacta
    27361 aaagaacaat tggaagatgt actagataca ttgactgatc gggaagagaa tgtccttcgc
    27421 ttacgttttg gtttagatga tggccggacg agaacattgg aagaagtagg gaaagtgttt
    27481 ggtgttactc gggaacgtat tcgtcagatc gaggctaaag cattaagaaa attgcgccat
    27541 ccttctcgtt caaaacaatt aaaagatttc ttagaataag cgtggactat tgaccagaag
    27601 agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa a
//
LOCUS       DANJMA010000031        27455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000031 DANJMA010000000
VERSION     DANJMA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(38..634)
                     /locus_tag="PWU31_001622"
     CDS             complement(38..634)
                     /locus_tag="PWU31_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1084169.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="PWU31_001623"
     CDS             984..1328
                     /locus_tag="PWU31_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL1084170.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="PWU31_001624"
     CDS             1329..1748
                     /locus_tag="PWU31_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL1084171.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="PWU31_001625"
     CDS             complement(1840..2190)
                     /locus_tag="PWU31_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084172.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="PWU31_001626"
     CDS             complement(2156..3487)
                     /locus_tag="PWU31_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL1084173.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="PWU31_001627"
     CDS             3742..4308
                     /locus_tag="PWU31_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1084174.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="PWU31_001628"
     tRNA            4453..4540
                     /locus_tag="PWU31_001628"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /gene="bsh"
                     /locus_tag="PWU31_001629"
     CDS             4876..5850
                     /gene="bsh"
                     /locus_tag="PWU31_001629"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL1084175.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="PWU31_001630"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="PWU31_001630"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL1084176.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="PWU31_001631"
     CDS             complement(8265..8711)
                     /locus_tag="PWU31_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL1084177.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="PWU31_001632"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="PWU31_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1084178.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="PWU31_001633"
     CDS             9176..9694
                     /locus_tag="PWU31_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1084179.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="PWU31_001634"
     CDS             complement(9771..10727)
                     /locus_tag="PWU31_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1084180.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="PWU31_001635"
     CDS             10893..11699
                     /locus_tag="PWU31_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084181.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="PWU31_001636"
     CDS             11696..12685
                     /locus_tag="PWU31_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1084182.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="PWU31_001637"
     CDS             12682..13686
                     /locus_tag="PWU31_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1084183.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="PWU31_001638"
     CDS             13818..14360
                     /locus_tag="PWU31_001638"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL1084184.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="PWU31_001639"
     CDS             14380..15078
                     /locus_tag="PWU31_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL1084185.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="PWU31_001640"
     CDS             15099..15311
                     /locus_tag="PWU31_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL1084186.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="PWU31_001641"
     CDS             15311..16273
                     /locus_tag="PWU31_001641"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL1084187.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="PWU31_001642"
     CDS             16291..16686
                     /locus_tag="PWU31_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1084188.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="PWU31_001643"
     CDS             16849..17214
                     /locus_tag="PWU31_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL1084189.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="PWU31_001644"
     CDS             17211..19022
                     /locus_tag="PWU31_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL1084190.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19251)
                     /locus_tag="PWU31_001645"
     CDS             complement(19084..19251)
                     /locus_tag="PWU31_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL1084191.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19500..20489
                     /gene="galE"
                     /locus_tag="PWU31_001646"
     CDS             19500..20489
                     /gene="galE"
                     /locus_tag="PWU31_001646"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL1084192.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20501..21985
                     /gene="galT"
                     /locus_tag="PWU31_001647"
     CDS             20501..21985
                     /gene="galT"
                     /locus_tag="PWU31_001647"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL1084193.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22009..22989
                     /locus_tag="PWU31_001648"
     CDS             22009..22989
                     /locus_tag="PWU31_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084194.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23180..23902
                     /gene="tsaB"
                     /locus_tag="PWU31_001649"
     CDS             23180..23902
                     /gene="tsaB"
                     /locus_tag="PWU31_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL1084195.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23886..24428
                     /gene="rimI"
                     /locus_tag="PWU31_001650"
     CDS             23886..24428
                     /gene="rimI"
                     /locus_tag="PWU31_001650"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1084196.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24435..24899
                     /gene="rimI"
                     /locus_tag="PWU31_001651"
     CDS             24435..24899
                     /gene="rimI"
                     /locus_tag="PWU31_001651"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1084197.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24896..25912
                     /gene="tsaD"
                     /locus_tag="PWU31_001652"
     CDS             24896..25912
                     /gene="tsaD"
                     /locus_tag="PWU31_001652"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL1084198.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26484..27185)
                     /locus_tag="PWU31_001653"
     CDS             complement(26484..27185)
                     /locus_tag="PWU31_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1084199.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8938 a         4705 c         5656 g         8156 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    19261 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    19321 tttgtttgta aacctttgca aaaaatcctt gaaaatgttt actaaatgtt ttattataaa
    19381 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
    19441 tttttttaaa ttatatataa tcaacaaaca caattttact aatcaaagga gtgtttttta
    19501 tgacaatttt agtactaggc ggagccggtt atatcggttc gcacgctgta gaccagctag
    19561 ttcaaaaggg gtatcaagta gctgtggtag ataatttgct cacaggtcac aaacaagcag
    19621 tacatccaga tgctcatttt tatgaaggag acatccggga taaagaattt ctacgttccg
    19681 tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc tgcaagctct ttagtaggag
    19741 aatctgtcga aaaaccattg atgtatttca acaacaacgt atacggtatg caaatcttac
    19801 ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt ctcttctact gcagcaactt
    19861 acggagaacc aaaagagtct ccaatcacag aagatacacc agctaatcct aaaaatccgt
    19921 atggtgaaag taaattgatg atggaaaaaa tgatgaagtg gtgtgaccaa gcatatggca
    19981 tgcgctatgt cgctttgcgc tacttcaatg tagccggagc aaaagcagat gcatctatcg
    20041 gagaagatca tacaccagaa acgcatcttg tcccaatcat tttacaagta gctcttggtc
    20101 aaagaaaagc attggctgtt tatggagacg attatgatac accagatgga acatgtatca
    20161 gagattatgt tcaagtagaa gatttgattg ctgcacatat tttagctttg gaatacttga
    20221 aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag caaaggatac tcagtcaagg
    20281 aaatgctaga agcagctaga gaagtgacag gaaaagaaat tccagcagaa atagcaccaa
    20341 gaagagctgg agatcctagc cgattagtcg cttcaagtga aaaagcgaga gaggttctgg
    20401 gttggaaacc tgaatacaca gatatcaaag caattatcaa aacagcatgg gattggcatg
    20461 tcagccatcc aaatggttat gaagaatagg ggaatgagat atgtcaatca gtcaaacgat
    20521 ttgtgatttt gccacacttg cgatcgaagc tggcggatgg atggaaatgg accgcttata
    20581 tttacagaat cgtcttttag ggatggtagg agaagatgct ctagaaagca ttgaagtaag
    20641 accagttaag gaagaatctg tagacttatt ggatcagctt gtcgctaagg caagagaaaa
    20701 tggtgttctt tcagaattgc cagcagaaca agagattttt gaagcgcaat taatggattt
    20761 tctcacaccg ccaccttcag tcgtcaatgc attctttgca cagcactatt cgaaaagccc
    20821 agaggaagca actgagtatt tctttaattt aagtaagaaa aatgactaca tcaaaacaag
    20881 agccatcgca aaaaacatca aatttccggc agagtcggag tatggtgaac tggaaatcac
    20941 aatcaattta tctaagccag aaaaagatcc aaaacaaatc gcagcagaac gcgaggcggt
    21001 aaaagtagac tatcctacct gcatgctttg catggaaaac gaaggatata gaggtcgctt
    21061 gaactatcca gcccgcacca atcaccggat tattcgaatg aatctagacg gtgaaagctg
    21121 gggattccaa tattctcctt atgcttatta caatgaacat tcgatcattt tatcagaaca
    21181 acaccgcccg atgaaaatca caaaggaaac atttgctaga ctgcttcgga ttactgaagt
    21241 cttgcctcat tactttgttg gttcgaatgc tgacttgccg attgttggag gatcgattct
    21301 ttctcatgat cattatcagg caggacgtca tgaatttcct atggcaaaag caccgataga
    21361 aaaacttatc aaactagcag attatccgca agtcatcgca ggagtcgtga aatggccgat
    21421 gtctgtgatc cgtttgcaat caacagagaa aaaattgttg attgaagcag ctaactcagt
    21481 attggaaaag tggaaagcat attcggatga aacagtatct atcagagctt tttcagcaga
    21541 tggtacacca caccatacga ttacgccaat tgcacgaaga agggacaatt actttgagtt
    21601 ggaccttgtc cttcgagaca acaatgtttc agaagaacat cctgatggga tttttcatcc
    21661 acataaagat gtccaacata tcaaaaagga gaacattggg ttgatcgaag taatggggct
    21721 tgctgtttta ccacctcgtt tgaaaccaga actagtagaa gtagagcatt tcttattagg
    21781 agaagaaaac cagatggctg aatatcatcg tccgtgggca gaacaattaa aaaaagaaca
    21841 tcctgatttg acgaaagata cggtcaccga tgtcgtacaa aaatctgtag gtcaggtttt
    21901 tgcacgtgta ctggaagacg ctggagtatt caagcgagat gaaaaaggac aggcagcgtt
    21961 tgaacgattt actgcaattt tataaacgta aaaaaggagg agagacaaat ggcaacaatt
    22021 aaagacattg cgcagttggc aggagtttct cctgctactg tttcaagagt tctcaactat
    22081 gatcctgatc tttccgttgg cttagccaca aagcaaaaaa tttttgaagc agcagaacgc
    22141 ttaaattata cgaaacatcg taaaaatgca gcaaatacta agcaaacgct tcgcctgatc
    22201 cagtggtacg acgatacgga agagcttgaa gatctttatt atttatctat tcgtctagga
    22261 atcgaaaaaa aggcagaaga agcaaacgtt actttattga aagaaacgtg ggattccatt
    22321 agtgatcttc cgacagatgg aacgatcgca ctaggaaaat tcgacgaagc tcagttatta
    22381 gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt ctgacggcac ttctcaagga
    22441 atcgactctc tagttgtcga ctttaagtca agtgttcaaa aagtcattga acattttgtc
    22501 tcccataaag cgcaacatat cgctatgctt gctggaacag aatatacgaa gaaaaaccat
    22561 cagcggctaa aagatcccag aattatggca tttcaacaag ttttggcaga aaaaggctac
    22621 gacgaaggtc caattatcga agcagatttt tctgtcgagt caggatatca agcagtgaag
    22681 cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt ttgcagctaa tgatgcatta
    22741 gcgattggcg cactgaaagc gattcaagaa aaaggtttag tggtaccaga agatatttct
    22801 gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa cgccctctct ttctactgta
    22861 aaagtttata ccgaatggat gggagagcta gcagtggaga cgattcttca attaagtaat
    22921 tccaaagcac cggtttcaag aaagattatt gtggaaacag agttgattat tcgagaatca
    22981 acgaaataaa agatggaaaa gctgtgggga aatgaagtga taccgcagct tttttgtgtt
    23041 tcaggaaggg gcaaaatttg cccgccagca gataatcatg tatcattgta ctagttttgc
    23101 ctttggtatt ttcatcctca gaataaaaaa gtatgctaca atggaaggcg agaatgtcat
    23161 agcagaaagg atcaaatata tgattacatt aggaatagat actgcaaacc aaactttggc
    23221 gatcggtgtg gtagaagacg aacaaattct tggacaaata caaacaaata tcaaacgcaa
    23281 ccacagtgta acactgatgc ctgcaatcga ccaattgttt gcagatcttc aaataagtcc
    23341 aaaagacatt gaccgaatcg cggtttctga tggtcccggt tcttatacag ggcttcgtat
    23401 aggtgtaacg acagcaaaaa cgattgctta tacattagat aaagagctag taggagtatc
    23461 tagtctgaaa accatcgcgg ctaattgcat cggtgtggat aaaattattg tgcccatgtt
    23521 tgatgctcga agaaaaaatg tatacgctgg ggcataccgc tggaaagaaa atagattaga
    23581 aattgtactt gaagatgtac atatttctgc ttctgatttg ttcacacaac tacaaaagct
    23641 gaatgaagct atttatttcg tcgggagtga ttgtcacaaa ttcttctatg aaataaagga
    23701 aatattgcct agtgcgatga ttaatacagt tccagcatgg gatattccaa gtggtgtaac
    23761 tgtggctcaa ttaggtgctt tagctgaacc agttgcagag aaacaagctt ttcttcctcg
    23821 ctatttaaaa cgggtagaag cagaagaaaa atggttggag acgcacacac ctggagacga
    23881 aagttatgtt gaaaaaattt agagcgtatg ccagaagctt ttttcaagat aaaatgattt
    23941 atacagaaaa aacaatcgaa caaatcgatc gtcgttacgt actaagagaa ttgactacag
    24001 aagatatcaa agagctgctg gatgtcgaac gagaagtcta taatggcgaa ttgccatgga
    24061 caaaaagtgc ctttatatca gaattgaagt caccgcttcc tcatctatat ctaggtatct
    24121 ttgataatgg aagaatgatt ggttttatcg gttcaagagc gttcggttta gattgccata
    24181 tcacgaatat tgccgttcga ccggcatatc agcgtaaacg aatcggttcg ctgcttattg
    24241 acgaaatcga gaactttgct ataatgaatc gctgtgagac gatgtcatta gaagtgcgta
    24301 tgagtaatca agatgcacaa agactttatc ggaagttagg ttttcaagca acaacgatca
    24361 aaaaaagata ttacactgaa acaaaagaag atgcgttgga tatgataaaa cttttagaaa
    24421 atgagtgaga ttagttgatt tgcagaaaaa aagattttga tgaagcgtac ttggcagaat
    24481 gcgtatggat gattagcgaa aatgcttatc cacatggttc accttggacg aaaatgcagt
    24541 ttttggcaga catccaacag cctcactctg attatctgct tttagttgaa gataaccaga
    24601 tctgtggctt tattggatac tcgaaagtaa tagatgaagt cgagatcaca aatattgctg
    24661 ttgcagtttc agaacaagga aaagggcatg cacggcattt gttacaatta ctgattacgg
    24721 aacaaataaa agattcgctg catgtttttt tagaagtacg tctatctaat gaagcagcaa
    24781 gaaaacttta tgaatcagaa aagtttcgta tattaggtaa acggaaatca tattatcgca
    24841 atccgacgga agatgcgatc attatgagca gaaagttaaa aacggagacg atgaaatgaa
    24901 tgaagaattg attctggcga ttgaatcaag ttgtgatgaa acgagtgtag cggtcgttag
    24961 aaatggaaca gagatcttat ctaacatcgt tgcttctcaa ataaacagtc ataaacgttt
    25021 tggtggcgta gttcctgaag tagctagccg ccatcacgta gaacaaatca ccttatgctt
    25081 ggaagatgca ctggtagaag ctggtgtgtc ggcagaagat ttatcggcag tcgctgtcac
    25141 ttatggacca ggacttgtcg gctcgttgct gattggtatt tctgcagcta aggcttttgc
    25201 ttgggcacac caattgccgc ttattcctgt aaaccatatg gcagggcata tctatgcagc
    25261 ccgtctggtt aaaccattcc agtttccatt gatggcactt cttgttagcg gaggacatac
    25321 agaattagtc tatatgcaag aagatggatc atatgaaatc attggggaga caagagatga
    25381 cgcagcagga gaagcttatg acaaagttgg aagagtatta ggtctttctt acccaagcgg
    25441 aaaagaaatc gatcagctag ctcatcaggg aaaagataat taccattttc cacgagcgat
    25501 gatccatgaa gataattatg atttcagttt tagcggatta aaaagtgcat ttatcaattt
    25561 ggtgcataat gcacaacagc gtggagaaga tctagacaaa aatgatttag cagctagctt
    25621 tcaagcaagt gtgatcgatg tattgattac taaaacactt cgagcttgtc aaaattatcc
    25681 agtcaagcaa ctgattgtag ctggaggtgt agcggccaat caagggttgc gtgaaggatt
    25741 gcaggatgct ttggcaacta aactccctga ggtagaacta gtgattcccc ctcttcgact
    25801 ttgtggagat aatgctgcaa tgattggagc agcagcacat gtggaaatgc aaaaaagaaa
    25861 ttttgctagc tatcagttga atgctgatcc aagcttggtt ttatctgaat aaagaggttg
    25921 tgaaaaatct gccctgcatc aagtaataag aacagcccag gaaaaacggc ttctcatgtt
    25981 tttgacctgg ctgttcttat taccgcgtat gcaagctttt gaacgccgtt taatcaagag
    26041 ttcgtaagcc tgtcgtttaa ctctgattca caaggaacaa tttcaaaaaa cagctcctca
    26101 tgtttatgaa cattatgact tgtgaccgaa gagttgacag gtacacaccg tttaatcgaa
    26161 ataagggctg tgacacgctt ttgtcaaagc cctatttttt acagcttatt catcattatc
    26221 cgttcctctg attgccctca ctgattaatc gtttattgtc gaggaatatc gaataatcta
    26281 ggtcggatga ggacaagacg cttcctctag ttaatctatc agtatttttt gcaaacagtg
    26341 aatattacat aactgaaaca cacatagaaa atctttgagt cttgcccagt gatctaaaac
    26401 tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat aaatatcagg
    26461 gtcactgctt atttttgttc gcgttattct ctcatagctg gagtagtagc tgtcttacag
    26521 aaagtaaaac gctttttgtc ataatctaac agtccggaat cacgtagttg tttgattact
    26581 tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta attcttttaa ggtgatcgga
    26641 taaggaatgg tgatatgacc atcttttgtt ggtacgccca attcttcttt caaaatgaat
    26701 aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc cgatttggat acgcaattca
    26761 tgtatttcaa ctactttaga aattttttgt atcatataga tcaactgttc gggatttttt
    26821 cgtgctaatt tttcatagta ggaaacagga aggtagaatg tttcaatatc agtcatcgct
    26881 tgagctgtat aagtgtaggt ctcttctcca aacattcctg acataggaaa aaggttattt
    26941 gtattcacat agtcatagta gtaataagtt gcactttcat cataacgttc taaacgaacg
    27001 agtcctttgg tcaagatata caaccgttgt ctgggatcac catcgtcgaa aaggatctgt
    27061 ccttttttat aagaacgaag aacagtatgt tccttgatct gttggaactc ttcatccgag
    27121 aaatgttcaa aatcaggttg atatctgagt tttgaaaagt gaaaatcgat tttattaaca
    27181 cgcattaaaa agtcctctcc ttattgttca ctatatttat tgtcactttt tttaaagaat
    27241 ttgtaaagtt ttaacatacc aacattagaa acaagtttta atactttaca aatataattt
    27301 gttattatgt aaaaaatata caatggtatt gaataatttg ttttgaagcg aatgatttgt
    27361 gtataaaaaa caacaataat acatatagta aacgctaaca acgctaatat aatttatttt
    27421 tttaatttat tcaaaaaatt tttttcaatg aataa
//
LOCUS       DANJMA010000032        27298 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000032 DANJMA010000000
VERSION     DANJMA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(2..1066)
                     /locus_tag="PWU31_001654"
     CDS             complement(2..1066)
                     /locus_tag="PWU31_001654"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL1084200.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1063..2148)
                     /locus_tag="PWU31_001655"
     CDS             complement(1063..2148)
                     /locus_tag="PWU31_001655"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL1084201.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2161..3138)
                     /gene="mvaD"
                     /locus_tag="PWU31_001656"
     CDS             complement(2161..3138)
                     /gene="mvaD"
                     /locus_tag="PWU31_001656"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL1084202.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3131..4075)
                     /gene="mvk"
                     /locus_tag="PWU31_001657"
     CDS             complement(3131..4075)
                     /gene="mvk"
                     /locus_tag="PWU31_001657"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL1084203.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4391..5050
                     /locus_tag="PWU31_001658"
     CDS             4391..5050
                     /locus_tag="PWU31_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1084204.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            5135..6040
                     /locus_tag="PWU31_001659"
     CDS             5135..6040
                     /locus_tag="PWU31_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1084205.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6042..6674)
                     /locus_tag="PWU31_001660"
     CDS             complement(6042..6674)
                     /locus_tag="PWU31_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL1084206.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    6825..6950
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6994..7518
                     /gene="infC"
                     /locus_tag="PWU31_001661"
     CDS             6994..7518
                     /gene="infC"
                     /locus_tag="PWU31_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL1084207.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7590..7790
                     /gene="rpmI"
                     /locus_tag="PWU31_001662"
     CDS             7590..7790
                     /gene="rpmI"
                     /locus_tag="PWU31_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL1084208.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7843..8202
                     /gene="rplT"
                     /locus_tag="PWU31_001663"
     CDS             7843..8202
                     /gene="rplT"
                     /locus_tag="PWU31_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL1084209.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8554..9792
                     /locus_tag="PWU31_001664"
     CDS             8554..9792
                     /locus_tag="PWU31_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL1084210.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9840..11969
                     /locus_tag="PWU31_001665"
     CDS             9840..11969
                     /locus_tag="PWU31_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL1084211.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11944..12633
                     /locus_tag="PWU31_001666"
     CDS             11944..12633
                     /locus_tag="PWU31_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084212.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13041..13727)
                     /locus_tag="PWU31_001667"
     CDS             complement(13041..13727)
                     /locus_tag="PWU31_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1084213.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13863..14057)
                     /locus_tag="PWU31_001668"
     CDS             complement(13863..14057)
                     /locus_tag="PWU31_001668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084214.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14107..14547)
                     /locus_tag="PWU31_001669"
     CDS             complement(14107..14547)
                     /locus_tag="PWU31_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL1084215.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14551..15396)
                     /locus_tag="PWU31_001670"
     CDS             complement(14551..15396)
                     /locus_tag="PWU31_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL1084216.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15545..16963
                     /gene="pepV"
                     /locus_tag="PWU31_001671"
     CDS             15545..16963
                     /gene="pepV"
                     /locus_tag="PWU31_001671"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL1084217.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17039..18241)
                     /locus_tag="PWU31_001672"
     CDS             complement(17039..18241)
                     /locus_tag="PWU31_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084218.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18228..20015)
                     /locus_tag="PWU31_001673"
     CDS             complement(18228..20015)
                     /locus_tag="PWU31_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084219.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20028..20981)
                     /locus_tag="PWU31_001674"
     CDS             complement(20028..20981)
                     /locus_tag="PWU31_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1084220.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21166..21516)
                     /locus_tag="PWU31_001675"
     CDS             complement(21166..21516)
                     /locus_tag="PWU31_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL1084221.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21716..22795
                     /gene="pepA"
                     /locus_tag="PWU31_001676"
     CDS             21716..22795
                     /gene="pepA"
                     /locus_tag="PWU31_001676"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL1084222.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            22938..23258
                     /locus_tag="PWU31_001677"
     CDS             22938..23258
                     /locus_tag="PWU31_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1084223.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23280..23744
                     /locus_tag="PWU31_001678"
     CDS             23280..23744
                     /locus_tag="PWU31_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1084224.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            23940..24545
                     /locus_tag="PWU31_001679"
     CDS             23940..24545
                     /locus_tag="PWU31_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL1084225.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24584..25873)
                     /locus_tag="PWU31_001680"
     CDS             complement(24584..25873)
                     /locus_tag="PWU31_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL1084226.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26301..26780
                     /gene="rlmH"
                     /locus_tag="PWU31_001681"
     CDS             26301..26780
                     /gene="rlmH"
                     /locus_tag="PWU31_001681"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL1084227.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            26983..27261
                     /locus_tag="PWU31_001682"
                     /pseudo
     CDS             26983..27261
                     /locus_tag="PWU31_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         8640 a         5102 c         5490 g         8066 t
ORIGIN      
        1 tttacttttg tcccacgctc ttttctgtgc gtcttccata cttcactaag tcgatccctc
       61 ttgcttcgca ccaatccttc actggtccgg aaaagatcag agattgttgg tgtagggccg
      121 cggtattcgt tgcgccgacc atagtataaa gcagacgaag ctcttcttgc cattgcttca
      181 tcagtataat agtttcttct acgccgtgat tcatcagatg cgtcagtacg gttcctgaag
      241 tacccacggc atttgcgccg agacaaaggg ctttaaatat gtcataggca ttgcggatac
      301 ctccggaggc taggatttcc atagaagtgt ctgcttcatt tgcttcaagc aatgaagaga
      361 ctgtcgattg cccccagtca gctaaataac tcagttcccg cttggagcga cgggcgtttt
      421 cgatctgggt gaagcttgtt ccgcttcgac cgctgatatc aactgtatga actcctaaag
      481 cagctagatc attgagcgtt tctcttgtca tgccaaaacc gacttctttt acgatgaggg
      541 ggacgtcaat ggctgtttga gtttcttgaa taagtacttt ccaattggta aaatcacggt
      601 ctccctcagg cataaccaac tcttgaggtg cattcaaatg aatctgtaag gcatctgcgt
      661 gaaacaatcg gatagcttct ttggctcttt cgacagaagt ccctgcaccg atgttggcaa
      721 tgattttccc gtgaggatat tcttgtcgca taatcgtata agtatcggct aaagaaggga
      781 ctttcaaggc agcgctgact gaaccagtgg caatcatcaa atctgcttct cttgcgacga
      841 ttgcaagatc ttggttgatt ttttttgttt tttcgcttcc gccagtcatc gcattgatat
      901 aaaatgggct gcttagtgta aacccaactg cctgtgtact tagatcgaca tcagaaactg
      961 ctatttgcgg caaagggtta tggatgatcc gtacgaagtc aaattcgttt ttttgtttat
     1021 catgaaaggc tttagctagt gaaacatgtt cgtcttttcg attcatcggt tttcctttct
     1081 ttgatcgcta tagacatgta acggcagtgg agtgatttct gctttttccc atgcactcat
     1141 taaaggaaga atgccagatt tctggtcaac aattacgatt ccgcaatcgc ccccacctgc
     1201 accagaagat tttgcggctc cttcatactg ttcagctaaa ttacacaatt tgttcaaagc
     1261 aggcgtttcg atgacgaccc cagtgattgc agataaatca tgcagtaatt gtcggttttt
     1321 tcgaatcatc tgttggatca acgttacatt attttcttta aaccctttga tcatttcgtt
     1381 gacacattct gtactatttt ttaagaaaag ctgataagcc accattttat cttctctcga
     1441 acggtgaact tgatcgacca aatcagaagt agaggcaggg ctacccgtcc aaccaatcaa
     1501 taaacgtaaa tcttcaggag caatcaatgg ctcaatggat agacctggcc aatccagggc
     1561 aagtaactca ctgatagaat gctgagtttc ttgttcttgg agccaaggat ggtcgaaggt
     1621 tgagaaagcg atccagccac cataacagct agcagcgatg tcgccgcagg aaccattatc
     1681 ttgaactgct aaattggcta gtgctgcaat cttgaaaatc tccaactgag ataaattcaa
     1741 cgcgtaaaaa acattcaagg ctttgaccgt tgcaacggtc acggcaccgc tagaacccaa
     1801 tccatatttc cggccatttg aactgtctaa ctcacttgtt actttcagat cataaaagga
     1861 taaaagaatg tttttttctt gtgcatactt ttcagtcaag cgaatagcag caaggatata
     1921 atgaaaagga ttttcccgaa tatctaaaac taattctccg ttgcgtcttg tccaacgtac
     1981 aggcatccca ctatattgtg cagattggat acttccgact tttcgtgcag attctacagt
     2041 gactgtcacg aattgatcga ctgcagcgat aactgctgga tggcctgttt caacaactgc
     2101 atattctccg gcaatataga gtttgcctgg tgcagatact tcaatcataa aaatctccct
     2161 ttattcaata atcgcaattc ctggaccagc atgtgcagta attacttgtt ctttgctaaa
     2221 taatttggta aatgtttctt gtacctcaga taaatggctg ttttccacta aaactttgac
     2281 attagggcca gcatccatcg taaagtagca tggaatgcct tgctttctca attgtcgcac
     2341 cgcatccatc gcttttaagc tgtcaggaga ccagtaagtg aatggaggct gggcagctaa
     2401 agtcgttcca tgcattttta gtccgtttct ttccatcgtt tctccgagaa gttggaaatc
     2461 ttttgttttg attgcttgtt tcaattgata tagatcccct tcaacggaat ctaaccagcc
     2521 ttgataaaaa ctggatgttt cgactgtccg acgcatccca tttctgctgg acacttcttt
     2581 tttctggtcg ttgatcaaaa cgaaaaccat tgctaaatcg tcttcgaaag aatcggaagg
     2641 gactggctta gcgtaagaac ttaagtcgtc atggcctttt tcccattcga cgaaaccacc
     2701 gaaaatactt cggcaagctg aaccagaccc gcgtcgagca aatcttgaaa gagagagatc
     2761 atctaatcca agcttaagag cagtattgca agcgcctgct aaagcagcta gcccgctggc
     2821 agaggaggct aatccagctg cggtaggaac gaaattctga ctgattactg aagcttttag
     2881 tgagaggccg gcttctttac gaaaaaggtc aagaaactga ctgacttttt ttgttgcttt
     2941 ttcgtcttgc aaggtgccat ctagataaaa ttcatctacc atatagctat cagaaaagat
     3001 gacttctgtc tctgtataaa atgcatccaa tgttaacgaa aggctgttgt tcattggaag
     3061 gatgagttct tcatttttct taccccaata tttgattaga gcaatgttgg tatatgcgcg
     3121 tgctttgcct ttaaacatag gtatgtactc ctaatccttg tatccaagtt tcagcagcac
     3181 ccgcatcttc aagtgcttga ctgatttctt gggcttttgt ttttgtttgt gctaatgcaa
     3241 tcatacagcc accgcgtcca ccgccagtta atttggcacc caatgccccg gtatcttttg
     3301 ctgtttggat caatagatta agaaaatcat tgctgattgt cagcttttcc agaagtgatt
     3361 gtgattcatc cattgtctgt gccaacgttt ctggtgaatt ttcaatgatt gcttgttttg
     3421 cttgcttcgt caagtatcct aatttttgga tcatttgtcc agtttcatga ggctgggttt
     3481 caaagaggtg agcgacatct ttgacggctt ctcttgtttg tcctttgatt cctgtatcag
     3541 cgacaatcaa aaaagcatcg atgttcaaag aaaagtagtc gaaaggatgc ccttttgtaa
     3601 aataaatcgg ctgcaagctg ctagtagcgg ctgcatcgat tccactagga ttaccgtggg
     3661 cgattttttc cgaaagctgg acattttcta atagtatttc acgagacaaa ggaaatgcta
     3721 agtaatcata aaaagcacga gtgactgctg tggcgactgc agcgcttgat cccattccac
     3781 gttcagcagg aatcgtgctt tcaatggtca gttgcaagtt tttttggatg tcatgctgtt
     3841 tttttaagtg aatgaataat tttttgatat tttttaatgc atgaggagct tcggctaaat
     3901 ttcctgaata gtaggaagaa accagttgat cgtccattgc atccagctct tcaaggaatg
     3961 cggtgacttt tgttgcatag aaaggaaagg caatcgctgg ttctccataa acaaccgcgt
     4021 gctcgcccat caatatgatc tttccgcttg attctccttg gccatagttt gccataatct
     4081 aactccttct cacgtaaaag tgatttttaa gcaaaaaata aagcgcccaa aacgagttgc
     4141 gctgcttcac gaattttttt gtccactaag ggtctcaaaa aacattcctc ctttatttta
     4201 ctaaaaccat ctcagaagta aaactaagaa tacgttttct aaagaaaaaa cacttttttc
     4261 ttaagaattt tttgactttt tttcttcttt gactagaaaa aaatacaatt gacactacta
     4321 gatattgggg gtatatttaa aaaggttgga gttaaacaca atatttagtg atgaagtgag
     4381 ctggaggtaa atgagcgtga tcgttttagc aggtacaatt ggtgccggaa aatcaagttt
     4441 aacagaaatg atggctgaac actttgatag tcaggcattt tatgaatcga ttgatgataa
     4501 tgaagtcttg cccttatttt atgcaaaccc tgaacagtat gcttttttgc tgcaaattta
     4561 ttttttgaat aaacgttttg caagcatcaa acaagcaatg aaagatgaca ataatgtatt
     4621 ggaccgttcg atttatgaag attcactgct tttccatcta aatgcagatc taggaagagc
     4681 aacagaaaca gaagttcgtg tgtatgatga actgctggaa aacatgatgg aagaacttcc
     4741 ctatgctgcc cataaaaaac atccggattt attagttcat attcgtgtat cttttgatac
     4801 gatgttggag cgtatcgaaa aacgtggtcg ttcgtatgaa caattgtcct ttgatccaag
     4861 tctatacgat tattacaaag aattgaacag acgttacgat caatggtatg aagaatacaa
     4921 agaaagtcca aaaatccaaa tcgatggaga ccgctataat tttgtggaag atcctcaagc
     4981 aaaagaagaa gtcttgaaaa tgatcgaaga aaaattagca gagattcgcc aaaccaaagt
     5041 agccatttga tttacagttg ctcaaataaa gggtaaacct ttatttgagc gacttttttc
     5101 tttatactgg aaacaaatgt caggaggata gagtatgcgg gaaaaacact attttaatca
     5161 agaccgtaat ttttggatca atgtacagca aaacgatgtg ctagctgtta aaaacttaaa
     5221 agaagaatat ggcattagtg acgagatgct gacttattct ctagataaaa atgaacgggc
     5281 gcgcgtagag tacgattctt ttgatgaagc gttactgctt gtatcaaatg ttccgcacca
     5341 gcaaaaagtc gataatcatt atgaaactag tcctatcgct tttattttaa aagaagacgg
     5401 tttgtttaca tttaccacgc cgaataccga gtacgtgatt cgactaattc gatcgctttt
     5461 ggaacgaatg ccagacatga gtgtatacag tttattgttt cgtacattgt ttttgatttc
     5521 tgattccttt tttcctttaa tcgaggaagt gaatagtgaa cgtcagcggc ttaatctgaa
     5581 attaagagaa aaaacaacga acaagaatct gctgcaactt tcagatttag agatcggttt
     5641 ggtttactta gttacaggaa caaaacaaaa tgtagtactt ttagaacaaa tcaaggcact
     5701 ggctatttat cgcaagttgt cagaaaagga aaaagaagag ttggacgatg cactgatcga
     5761 agcacgacaa gcagttgaaa tgacgaactt agcttcgcag attttagacc agctctctgg
     5821 cacttataat aatttgctga ataacaactt gaatgacacg atgaaatttt tgacggtctg
     5881 gtcgctgatc ttgaccgttc caactattgt gacaggattc ttcggaatga atctccagtt
     5941 accctttacc cattccgttt tcggatggga aatcgccttg atcatcagtt tggtcttatc
     6001 tatttggatg cttattgcac tgtggcggcg gattcgttga tctagtcttt gatctgcaaa
     6061 ccaagggctt gggcgatcac aagtgcttgc tcttgattga cgattagtcc cgccaaagaa
     6121 tcttgagata aagcgattgt tgtgaagaaa ttagtcgtaa agtctaatcc cttcagagaa
     6181 gttcgaaacc agttcgaacc ggttaactga ttgtcagaca aaaaaaggtt tttccaagtg
     6241 acctcataaa attcgctgtc ttctaattga caatgatcga aggagacagc tttcaagttt
     6301 gtactactaa aggaagccat cgaagcaata caatcagtga aagtacagtc tcgaagataa
     6361 ctttcggcaa aattcgtacc tgttaattta cattgatgaa acacgacttg gtggaaactg
     6421 ccaccaatcc actccagatt ggaaaaatca catttttcaa aaatgacatt actgcattca
     6481 aaatgacgta aacctgtttt ttgcatcgta agtttttgga tatgactctc tcttaatacc
     6541 atatttttgg catcgagata gctttggtta cctccgttga tcagaagacc agagaagatc
     6601 tcctcatctt ctaaataaaa ctctcccttt gtaagaagag gaagagaagg tggaataggt
     6661 tcgatttttt tcatgtgggt tcacctcgtt tttctttagt atagcaaaga agcgggaatc
     6721 actgctagaa agcctgctaa tctaatcatt ttcagaaaag gaagacaaac tgtatgaaaa
     6781 acattgacaa aaagattagt ttttgctata ctataatggt tgagaaataa aagcagaagc
     6841 acccgcttct cgccttagtt gataatgtca ctgggctaga tagttttcac tggttctatt
     6901 aggtgaagta cgcgcgggtt ctgtaagaga actcgttttt ttattgccgt ttattcggta
     6961 agcttttctt tctctcataa tatttggagg tgaatgacca tagcaaagga tatgatggtt
     7021 aacgacggca ttcgtgcacg agagttacga ttgatcggtt cagatggtga gcaattagga
     7081 gttaagacaa aagcagaagc attgaatatt gcagaacaag caaacttgga tcttgtgtta
     7141 gttgcccctg gtgcgaaacc accagttgcg cgaatcatgg actacggtaa ataccgtttc
     7201 gaacaacaaa agaaagaccg cgaagcgcgt aaaaaacaaa aagtgatcaa tgtaaaagaa
     7261 gttcgcttaa gtcctacgat cgacttgaat gacttcaata caaaacttcg taatgcacgt
     7321 aagttcttgg aaaagggcga taaagtgaaa gcttctatcc gtttcaaagg ccgtgccatt
     7381 acccataaag aaattggtca aaaagtcttg aatcgcttag ctgaagaaac tgcggatatt
     7441 gcaacagtgg aacaaaaagc gaaaatggac ggacgcagca tgttcttgac gctggcaccg
     7501 aaaaacgaca gtaagtaatt tgaatgacta actggctgtt ggacagccga tagtcggata
     7561 acgaaagatt ttgaggagga aatatagtca tgccaaaaca aaaaacacac cgcggatcag
     7621 caaaacgttt caaacgtact ggtaaaggcg gattgaaacg cttccgcgcg tttacaagtc
     7681 accgtttcca cggcaaaaca aaaaaacaac gccgtcaatt gcgtaaagca agcatggttt
     7741 ctagtggcga cttcaaacgt attcgccaac aactagcaaa aatgaaataa gaagtacaaa
     7801 taacagaatc tgaagattta tttttattag gaggaattat acatggcacg tgttaaaggt
     7861 ggaacagtaa ctcgtaaacg tcgtaaaaga gtgctaaaat tagcaaaagg ttactacggt
     7921 tcaaaacaca ctttatttaa aacagcaaaa gaacaagtaa tgaattcata caactacgca
     7981 tatcgcgatc gtcgtcaaaa gaaacgcgac ttccgtaaat tgtggattgc tcgtatcaac
     8041 gcagcagctc gtatgaatgg cttgagctac tcaaaattaa tgcacggttt gaaagttgct
     8101 gaaatcgaca tcaaccgtaa aatgttagct gacttggctg ttaacgatgc agcagcattt
     8161 actgcattgg ctgaccaagc aaaagaagct ttggctaaat aagctaaaca attataaaga
     8221 ggaaagaaga taacgaaaag ttatgttctt tcctcttttt ttatttacaa tcctccatca
     8281 aatgaaacag gacaataacg tataagtcta aaataacata attaaggttt tttaattgaa
     8341 aactgaacat ttatggtcat cctttaatga aagcgctaac tacataagtt agaatgagtt
     8401 tgactgatat attagtttgc ttatcagaaa aacaaaatgg acggaggaat aacatgagtg
     8461 atattattat tggtattttg ttagttatct cattcttttt catggtctgg tactgtgtaa
     8521 aaggctacaa cttgatggtc ggcttttgtc atcatggcta ctgtatggat gggattagcg
     8581 ctagtcggaa acacgttttc accaaactcg gcgatggaag ggcaaggagt aattgatgtg
     8641 ttgacgcata tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc
     8701 ttctttggtg cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt
     8761 cgaaaggtag ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt
     8821 gtaaccgctg ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt
     8881 attgtgttgc cgatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt
     8941 atgggctcga ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg
     9001 atttatgctg gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt
     9061 ggtatgattg ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg
     9121 aacaaaaaga aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca
     9181 atgatctcat ggttagccgt attattacca gttttagggg tcgttctttt agatcttcaa
     9241 atcatcttag gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc
     9301 ggaggttata aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtcgat
     9361 gttgcaccaa tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat
     9421 gcttctcctt atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg
     9481 gcaatcgtat ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc
     9541 ggttcaggct cagctatttt agctgtcgtg ttagctgtta atccaacgat gtcgccagcc
     9601 tttttgttcc cgttatttgc aattacaacg attgctcctc agcatttaga tattacacaa
     9661 tcttgggttg cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa
     9721 tccatcccaa ctggttggat catcggtgcg atcttatgcc tgatcacgtt cttattgtac
     9781 gggaacgcct agtaaacaaa acgtcgataa aaacgattag ataaatggag gaaaagaaaa
     9841 tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa
     9901 tcgataatgc gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg
     9961 tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg gaaaataata
    10021 tcagtccaga agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga
    10081 ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt
    10141 ttttagcaaa acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag
    10201 agattgtcaa tgcattctta cctgtcaaaa acttaaatgt agaccgtgtt tcagaaacga
    10261 tttcttcttt agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag
    10321 ataatgggga accagaacga gttgtttatg aagcagcatc tgtgaaagcg atccctacaa
    10381 cgatggcttc tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa
    10441 tcaacgcatc tattttacca aaaatgctag atacggcaac ctcgacagaa gactccgtac
    10501 gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa
    10561 tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc cggtcctgct gcatcagtca
    10621 tgggctcttt gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa
    10681 caaccaatat cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg
    10741 gccacccaac atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga
    10801 tggtacgggc taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg
    10861 gtctagatta tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt
    10921 tctctcctaa agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag
    10981 aagtgactat tacaaataca tgtgcagcca atgttcttgg attagtgcaa gaagaacatt
    11041 tttcttatgg aaatgtccca tctgcacgaa aagccataca ggcattagca gactattgtc
    11101 atacgacggt tgaagacatc gcagagcaga tcatggaaaa atcctatgct aagattgaac
    11161 cggtgatttt agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag
    11221 gtgtaggggg cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat
    11281 attcgattcc tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc
    11341 gagatgttgt agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa
    11401 atgaagcgat gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg
    11461 tagaaatcga tccacatact tcaaaagtaa cggctatcgc tactggttct acggaagtga
    11521 aagcaaccga tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg
    11581 atatgcgatt aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat
    11641 gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat
    11701 ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga
    11761 cagctgtcga acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac
    11821 cagaatttta tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac
    11881 aactacagct tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg
    11941 tcgttgccgg aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag
    12001 atccccgaag aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt
    12061 cctttgtcca agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca
    12121 tgtagcatca aaaaaaagta cccttttgcc gatgtaggag aaatctgtga acaaatggct
    12181 gttccaatcg tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat
    12241 gcagaagatg aagggatccg attgatgacg gagccgttag aaaaattaaa atgttcagga
    12301 ctagcttcga tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac
    12361 cacatcgaag cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg
    12421 ttgccttttt atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt
    12481 ttttcaaaag agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt
    12541 tggccatata atgaaattcg tagccaaggg atcttagaag aaataaaaga aatagaaaaa
    12601 agatgtacgg agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca
    12661 gtcctttttt tccgaataaa cggtgttctc ctgtcaactc ctcggtcaca agtcaaaata
    12721 ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc
    12781 aggctcacga cctcttggaa aaaggtgttc aaaagcttgc atctgcggta ataagaacag
    12841 ccaagttaaa aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc
    12901 agctttttca caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca
    12961 aaaaaaacgg caaaatatga ggaactattt tgccgtttat ttctctaata ctcaggtcaa
    13021 aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg
    13081 gggtaattat gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa
    13141 agaaatccga cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca
    13201 tctaatactc gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc
    13261 aagtgcgtac tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa
    13321 caagttcggt gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgatcact
    13381 gcctgatcgg tttgttctag tattttttct agtttagctt gatcttgctt ggtcattttc
    13441 gcactgcatt cttgcaggat cggtttttcc aacttttccc gcgtataacg ggctaatgaa
    13501 gcagatcgga tatcgatttt tgaaacataa gtgccggact gtggaatggt ttttacaagc
    13561 tcttgattgc gcagttggat cagtgcttct cgaatcggtg tacgcccgat agctagttgt
    13621 tcttctaaca ctttatcgga gattttactt ccaggtgcta actcggaata aatgatttgt
    13681 ttacgtatag attcataagc ttgctgttgc aaattattga taaccattta accttccttt
    13741 ctatgcagat ataatctatt ataacataaa tcgcttacaa atcgtgtggg cataaaaaca
    13801 cagtctgcaa aaaaagccct gataggcatg cctattagag cctcgaaaag aaaaatattg
    13861 aatcaatctg gtcgtacctc ttggatcggt ttgacacgac cgccgattct tttagcaaaa
    13921 gcttcggatt cttttttgtc tttgaaaatg agcagattgt ctaagttttt ttcccgagtg
    13981 cgacagccag ttttatcgat gtaaccgtgc attactttaa cgacatacat ggggatcacc
    14041 tgcttttttc ttttcattat acgaactact cgacacaata caagaaaaat gcttagacgg
    14101 tatttattaa gaatttacat catccaaaga agcgtacgct tggaataact gttctaactc
    14161 cggattgact ttgctcagct tcactaatcg ttgcgaatca gaaagtaaaa aacagtgttt
    14221 actactgcca gtagttttta attttttgtc ttcagttccc acaatttcgt agctaaaatc
    14281 caaccgtgtt cccgtatact tttggatagt tgggtggatg ctcacttttt ctccgtaatg
    14341 gatcatttct ttatagctgc aatttacttc cagaacgggg atgatgatcc cagattcttc
    14401 gattttttta tagggaaacc ctagatgtac caataaggct acccgagcct cttcaaacca
    14461 gcggatataa ttgctgtgat gaataatccc catttgatct gtttcataat aaaaaggttc
    14521 tcgcacataa cctgtaaatt tctccatgac tcatgcctcg ttttccattg attttttaca
    14581 aaaataattc atccacttcg gaagtgcttc gctgatttgt gtcggcaaaa caatatattg
    14641 ttcttttgcc agctggtctc caatgaaact atgaagataa acagctgctg caatcgtttg
    14701 gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc ccagctaaag tgtcacccat
    14761 gccgcctgtt gccatagcag cagaacctaa aggattggtg tagaagtgct ggtgaccata
    14821 aatttttgtc tcatgacttt tcaaaacgag cacagcaccg atttcctgtt gcttcttttt
    14881 gttttcttta tcccgttgtt cttgtatcgg tagagaactc aaccgttgcc actccatttg
    14941 atgaggtgta agtacagttt gttcgggata gggtagcaaa agatgattag caacaagtaa
    15001 acttaaagca gagccatcaa tcaccagcca ttgttctttt gattgttttt ctagtacttg
    15061 tttgaaacgt tccaaactct tagaagaagt acccaatccg ggaccaatca agagtacatc
    15121 tgcttgcttt attacggaaa gaaactggtc agtatccgtc caatctacaa tcattgcttc
    15181 tggcagatga ctgtgaagag cactccgatt tttttcatca gttactacag tcgtgagacc
    15241 cgctccactg tgtacggcag ctaaagcact cataatgatt gctccgccat attgttcatt
    15301 gccgccaatc aaaacgatac gtccaaatgt tcctttatgt gaaatctttg gacgaggtgt
    15361 gatgactctt ttcactagtt gttcgtttat cagcatcgaa aaacctcctt ttactcatta
    15421 tagcgtatat tttttttaga gaaaaacaga taagaaaagg aattgtcaga ttttttgaac
    15481 tatttttttc tgtaatttgt ggtatcatga acacgaagac ttaaataagt ggaggattaa
    15541 ctttatgaca attgattggc aaaaagaagt agaagcacgt aaagaagact tgcttgaaga
    15601 cttgaaaaat cttttacgtg tcaacagcga gcgcgatgat tcaaaagtaa caccagatgc
    15661 accatttgga cctggacctc gcgacgcatt gaaacatatg ttagcttatg gcgaacgtga
    15721 tggatttaaa gtaaaaaatg tcgataacta tgcagggcat attgatcttg gcgaaggaga
    15781 cgaaacgcta gggatatttg gacacatgga tgtcgttcct gcaggagatg gctgggacac
    15841 agatccttat gaaccagtaa tcaaggatgg aaagatcttt gcacgcggat caagcgatga
    15901 caaaggtcca agtatggcag cttactatgc gatgaagatc attaaagaac ttgacttgaa
    15961 actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa gaaagcggct ggggcgatat
    16021 ggcttattat ttcgaacatg aagaagaacc agattttggt ttctcaccag atgctgaatt
    16081 cccaatcatc aatggtgaaa aaggaaatgt ttctttagct ttacgcttca aaggcgacaa
    16141 tgcaggagat tatgtcttga aatcttttgt ttccggactt cgcgaaaata tggtgccagg
    16201 aacagcaact gcagcacttc aagtaccaag tgcagatgca gctatcgcga tggaagaagc
    16261 attttaccaa ttcatcgaag caaatccagt aagcggaacg attgaagcgg acaatacgta
    16321 tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca agcccacaat ctggtatcaa
    16381 tgcaggttct ttcttggcat tgttcttaga taactatgaa ttcctaggat ctgctaaaca
    16441 atttatccat gtagcagctg cttatgtcca tgaagatttt tatggagaaa aactaggagt
    16501 cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat gctggactat ttgcttttga
    16561 agaaaatggt acagaggaag cgaacttcat caatatgaat ttccgttttc caaaaggtgt
    16621 aacagtagac ggattgcaaa gtgacatcga acaaacagtg gggcaagaag gcgcaacagt
    16681 tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca atggatgatc cattggtggc
    16741 tacattattg caagtctatg aagaccacac tggagaaaaa ggctacgaac aaatcatcgg
    16801 tggcggaact tacggacgtt tgttgaaacg tggcgttgct tatggagcga tgttcccagg
    16861 atatactgat acaatgcatc aagcaaacga atttatgagt cttgatgact tgttcagagc
    16921 aacagcaatc tatgcagatg ccatctatcg tttggcgaaa taaaaatact aaaaaactcc
    16981 ggctatctct gacagctgga gtttttatgt ttaaaaatga attagtacat cggtattctt
    17041 aaaagtaatt tagttcgtct tttgatttct caggagggat caaaaaggcg aaagcactaa
    17101 gtatagcaaa acttgtgatg atttgcgtac gatacgtaaa ccaataatga atctgtgagt
    17161 gattagctaa aatattgtac caaatatacg gcgttacagc agctaaaaca aacaaaacta
    17221 gaagttggaa cggacgccag ctatttcgtt tacgataagc aaaatagaga aaaacaagac
    17281 aagtaatcaa tagcaataat attgctactt ttggatacat caatctaata ttgcttttca
    17341 gcatctctaa tcgatgcaac gggtatttgt cgtttccttc cgttcgaaag aggatttggt
    17401 tgattgcatc ttttacaatg tttttgttca atatgatcga tgataggatc catttagcca
    17461 cccatgtcaa accatagcct aatccccaaa agataccggt aaaaacaaaa ctgcctactt
    17521 gtttgctcaa tgaactgcgt ttatccatta aatacttatt tgttacataa taaataatga
    17581 tcacaggtat cccccaagag atgaccggtg ttgtcaacaa gtcaaaaaag ttagctaaag
    17641 aaccaatgac aaagaaatac aagaataatt gttggttggt gttggagagt tgtggttttg
    17701 ttaaaaatag taatacagac aataaagtca aaatccaaat atttgaaaat tgcatactga
    17761 agaaaaaggt tgcagcattt cctatagcaa gcgaaatcaa caaggctgtc gttaaataga
    17821 tgtcgctaca cttggttaaa aagtagctcg tcaatccaat cagcagaaaa agaatggctg
    17881 cgtaaatcat tcgaatactc cccaaactca tccctagcag taaaggtcgt aaaaaaagct
    17941 gataaccatg ccaataacgg ggataatccg ccatagacat cgcgttctcc aaggagttca
    18001 attcaggatc tgcttttgtt tttcgtatca tcaaatgatc agtaaaatta tctaaccgag
    18061 tgcctagcat atttccttga ttgatagaag gatacattcc ttctttttca accatctgta
    18121 gagatttttc tgcattcgat tcaatagagt gctgcggaat gaaaaaagta attgtcagta
    18181 gtacgcaata gctagcgatc aagagaacaa aagcaaaaaa tatcttttta aatagtttca
    18241 tgagtatctc ctatatctgt tttcattttt tctcttttat ctagtaaaaa aggagagagg
    18301 atataaacag gtagacctaa tgctaaaaca aacacatacc gtaaactaaa agccacagga
    18361 gtagcaatca taatagtagc aaaaacaagc ataccgggaa gaaaaaggag taaataccga
    18421 tattttttct taaagattgc taaaagtgca ctgccgatag ctaaccacag taatgtgccg
    18481 gaaccaaaga aatcacgagc atcgatgatt tttttcattc ctgacaatcc agtaagatgt
    18541 tcaatcaaat ctacttgctt cataccataa ttattcgtgt ttacacgagt atctaaaaag
    18601 ccgtaactat tttttgtacc aggtacccag aacccgaacg tttctaaaat ataagcatct
    18661 gtatagattt ttatattact tggtaaattt cttgcccata gtgatagaaa ttctttttta
    18721 tgtgtattca aaaactcatt gttaaaattt ttatgccact tgattgagtc tgcaagacta
    18781 ggagaatacg cttcataatc ttttatcggg agtagctgat tcatgaatgc tttatctttc
    18841 ttagacaagt caccatccat aacaatagtt ctagaaattt gctgcaaagg aacacctaat
    18901 tgttcagcag tttctgtagg aatagcataa cgagccatca acgggtggat gagtagtcca
    18961 gcaatcactg tataacccag caccacttga aagaatgctt gatacctttt ttgtttcaaa
    19021 ataatcaagc cgcaaatcaa acctgtgccg gttactacat aaatcccatt atttcttaga
    19081 taggtggtca gtaaagcaag gatgattacg taaatctgat tgagaatcgt tggcttttct
    19141 cgttgaaaaa taaagtcaaa aaccgctaag gaaaataaaa agcaagcgat agagaacaaa
    19201 gtatcttttt gcatgttcaa ggcgtagatt ggaaaaaccg gtgcaaatgc aaaatagcat
    19261 aaactaatga gtaccggaaa aaaatggatg tttcttttta acagccagca aagagaataa
    19321 cttatcgctg aagctgttat caatgtttga aaaatcgaaa ataaaaatac accgtgattg
    19381 atcgtatcag ttggtaaatc atgaacaaat agcttgacca gtaaggtgaa taagactgga
    19441 tgatgattgc ttataggaac atcacccata gattgggcga ccgaacttaa cgaatcaggc
    19501 aaaaccactc caggatacaa tgccaacaga taaggaagcc aacacagaac taaaaagaca
    19561 gtaaagaata aaaaatattt tcttgaagag aaagcctgtg tgttttgagc agtaaaaaat
    19621 gtttccagtt ttggcattga taaataaatc gattgtaaaa ataaaaaaga atacacaaaa
    19681 atggagaaat aaacaagaaa gtaaaaaata cttccatcag caatcgttgt ctttgtatag
    19741 tttgctgaga catactgaaa ttgtatctgt ctgccaatag aaagtgtaag agcaaaagct
    19801 gcagaaaaaa acaatcgggc aatagctgca actttgtttt ttattgaaaa caactctatt
    19861 ccatattgct taaaagcaag tagcaaaacc atggctaaac caatgattac tgttggattc
    19921 aaagttccta gaccataaga agtaaaaaaa gcaaaacctg acgcaaataa agttgcaaaa
    19981 aaagtaggaa atagattctc ctttttctta tgcataaaaa aggcctccta cttcatttct
    20041 ttattttgat attcgtggta gacatggtat aaattcaatt catattcttt cttggcattt
    20101 gtgacgaccg tatccaaaat caaccctgtt actaatgaaa gaagggctaa agtcatcaat
    20161 ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat atattcacga
    20221 atcacaggaa tccccatgac caatccaaat aaaaagaaga agatggtcca gatcccaaag
    20281 aaaagcaaag gtttataatc tttgcacatt ttgatgatca tcatgatcac tttgaatccg
    20341 tcagagaatg tattcaattt tgattcgcta ccttcaggac gatcgcgata atcgattgga
    20401 atctcaacga acttgaattt tttatctaaa gcatgaatcg tcaattctgt ttcgatttga
    20461 aaaccagaac tcataatagg gaagcttttg acaaaaattc gattgaatcc tcgatagccg
    20521 gtcatgacat cgcgaatttt tgttttatat aagtaagtaa ttgtattttt aaccagatta
    20581 ttcccaaaat catggaaagg acgcttattt tcatcaaaat aggttccatt tgatagacga
    20641 tcgccaatga ccatatctgc ttcgccagaa cgtaatttct caagcaaacc atgtgcagct
    20701 tctgcaggat aagtatcatc accatctacc atcaaataat aatcggcatc aatatcaaaa
    20761 aacatttgac ggattacatt cccttttcct tgacgtggtt ctttctttac gattgctccg
    20821 ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac ataaatatct
    20881 gcttctggta attccctttt aaaatcttca ataacagttg aaattgtcgc ctcttcgtta
    20941 taacaaggaa tgagcacggc aattttttca cgtgattcca tttgtcactc tccttatata
    21001 ataaaactaa tcttatttca ataataccgt ataataacta caaatactag ctattccaag
    21061 aggaaatagc cctattttta taaaaatcac ccactataaa agaaggatag gatacctctc
    21121 ttttagatag tgggtgcgta ttcgttattt catttttttg acgtattaat ctattgcgcc
    21181 cttttttctt gaaatgtcta atacggttcc tgtatgagca tctgctaaaa attcataata
    21241 aacagtttcg ccatcttcta tccgggaaag cccaccccga taagcactcg accgaacggc
    21301 gaatttccga ataggttctt tttcaaaaga gatccaagca ccttcgattg cgccttcttt
    21361 taaaaaagat gttttgatat tttcaagcac ttggtctgct gataacactt ttttgcgatg
    21421 ataccaaatc gttgaagcta ccccactcaa taatcctaga ccgatcccaa cggctaaccc
    21481 gcctttaaaa tagcttaatt cgctttcttc atacatgtgg atatacctcc ttcttctcac
    21541 ttgctactct ttgtgtttat tttaccacac tctcatattt tctttgaaat aaataaccgc
    21601 taccagaaaa aataagctat aataaatacg atcggtctgc agaaaaaatc atattttatc
    21661 cgcagatcaa tataagtctg agaggagttt agtctgaaat ttagacgaat agcatatgga
    21721 agaaaaaaca tttcaacgaa tcaaagagtt aacagaatta caaggaacaa gcggttttga
    21781 acaagacgtt cgcgcctata tggaaagtca tatggaaccg ctggtagatg agttacagtt
    21841 agatggtttg ggcggcattt ttgggctgcg ccaccatgaa gaagcggatg cacctcgtgt
    21901 aatggtcgca gcacatatgg atgaagtcgg attcatgctg acacaaatcc aagaaaacgg
    21961 gctttttaaa gtcgtacctc taggcggatg gaacccttac gttgtttctg ctcaacgttt
    22021 tacacttaaa acctctactg gtaagaatta tccatgtatt tcttcttccg ttcccccaca
    22081 tttattacga ggaacaagcg gacaaaaatc agtagaagta acagatattt tgttcgatgc
    22141 tgggtttgaa tcaagagaag aggcaatgag ttacggtgtt cttccaggag acacaatcgt
    22201 tccttacgct gaaacgatca aaacagccaa cgggaagaat atcatcagca aatcgtggga
    22261 caaccgctat ggctgtacaa tggtattaga agctttagaa gcgttacaaa atgaaacatt
    22321 aggtcataca ttgattgcag gcgcgaatgt ccaagaagaa gtaggcttgc gtggttctaa
    22381 agcttctgtc aataaattca agccagatct gttctttgca gtagactgct ctgcagcaga
    22441 tgacacagtg acgaaaaatg gcacattcgg acatttagga gaaggtacat tgatgcgtat
    22501 ccaagatcca ggtttgatca tgcttcctcg tttgcgtgag tacttgctag atatcgctga
    22561 aacaaacaac atcccttatc aatactttgt ttcaaaaggc ggtacagatg caggtgctgc
    22621 tcatacacaa aatgaaggaa tcccaagtac agtgattggc gtagtgggac gttatatcca
    22681 tacgcatcaa acgatgttca gtattcgcga ttttgaagct gcacgagaaa tgttgattca
    22741 aacattgaaa ggtttagata aatcaacagt gaacacgatc gtatacggga aataacgcga
    22801 tagtgaaact ataaaatctg gcaaaagcaa ccttttgtca gatttttttt ataaaaagag
    22861 gatcaatgtt tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag
    22921 aaagtagagg aaaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag
    22981 aaactggaaa aagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca
    23041 aaccgcaaat gcctgaaatc gaaaatgact tttctgattg gcaatttatt gaagtagacc
    23101 gtgacaagta catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg
    23161 tgatccaaga cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg
    23221 aaatcgaatc gttcctagaa agtgtaataa aaggctgaca tctgttagga ggggtcaaga
    23281 tgaatcaaga atataagaac attttagtag gtattgacgg tagtgaacaa gcacttgctg
    23341 ctttcaaaaa agcgttagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg
    23401 taatcgatca gcaattttat aattttatgg gctatgctcc tattgatcaa aatctagtcg
    23461 atcagcaaac tgaaacagcc aagcaaatga tcgaagaatg taaagaatat ggaaaatctg
    23521 ttgattacac aaatatagaa ggaattattg catacggctc tgttcgtgaa tccatggcag
    23581 tcaaattgcc aaaaaaatat gcaatcgatc taatcatggt tgggcagtct ggactaaatg
    23641 cagtcgaacg ctttatgaca ggaagtgtag ccagctacgt catacgtcat tcaccttgtg
    23701 atgtcttgat cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta
    23761 taaccttttt aatcaaacag taaagggact gtgacaaaag tcttgtcaca gtcccttgtt
    23821 acgaataaat ggtgttccag aagaagcttc ttcggaaata aagcatgaaa aaaatctcat
    23881 gcagtgaatg caattttttg ttaagataaa tctcagcaaa ggataatgga ggaaagaaca
    23941 tgatttttgc ttataacaaa gcacacgtcg gagacacttt gatggttatc atagccgacg
    24001 ataaaggaac agaaaacact gtcgaacgta aatggaatgt tgctcggatc aaagatgaac
    24061 aaggtcaagt tgttgcttgg aactttttcc atatctccga tcatctaaca attgaaggga
    24121 acggccaagt aagcttaaca gatgaagaat tagcgaaatt gaatcgattg attaaagaag
    24181 caggattcga agaacagctt gaagctgatc atgaaccgaa gatcgttgtc gggtatgtaa
    24241 aaacatgcgt cccgcatcct gactctgacc atttatcgat tactgagaca gaagtggaca
    24301 atgggacagt attgcaaatt gtttgcggtg caccaaatat cgaagctgga caaaaagttg
    24361 tcgttgcaaa accaggagcc atgatgccag atggaatgat gatctggcca ggaactttgc
    24421 gaggggtagc cagctatgga atgatttgtt ctgctcgtga attgcatttg ccaaatgctc
    24481 cagaaaaaaa aggaatctta gtattgcctg aggatgccgt agtcggagaa gtatttccaa
    24541 aataaagagg ttgtgacaag tctttgtcac aacctctttt ttattaattt tcactatttt
    24601 gattttgttt taatgccgat tgatcgatcg tcaattcaac agtagttgtt ttttctttcg
    24661 agccttcata gtaagtgatt ttgacagaat ctcccacttt tttcttatac aaagcagatt
    24721 gaagttctac accagaagaa acttctttgt catcgatctt agtaatgacg tcgtattgtt
    24781 tcaacccagc tttttctgcc ggtgtagctg tttggacatt tgtcacgatg acaccgttag
    24841 ttacagatga aggaatcttc aagatttgtt cttgttgctg agtagaaacg gcagataagt
    24901 cgaccattgt gatacctaga gctggacgtg tcacttttcc gtctttttcc agttggttga
    24961 tgatattcac tacatcgtta cttgggatgg caaatcccat tccttctacg cttacattag
    25021 aagtcgattc agaagtactt gcgatcttac ttgagttgat accgataact tgtccttcga
    25081 tattcactaa aggaccacca gaatttcccg ggttgatcgc tgcatctgtt tggatcgcat
    25141 tgatattgac tgtttcgttc gattcattcg tacttgttac ttgtcggttc aaggaagaaa
    25201 tgattcctga agtgacagaa ttcgcatatt cagatcctaa tggggaacca atcgcaatcg
    25261 caggttcacc aaccttcaaa gcacttgagt ccccaaaaga ggcaaccgtt tcgactttgt
    25321 ctgaattgat cttcaacacc gctaaatctg aataggcatc tgttccaact aattcagctt
    25381 ttaccttcgt accgtctttc atcatgactt ccaaaccttg ctgtccatct actacgtgat
    25441 tgtttgtgac tacataagca gtattcccgt cttttttgta gattacgccg cttccttcac
    25501 tataagcttc caaattacta tcatctgagc tgctactttc ttcttgctgc ccgaataatc
    25561 cgccaaaacc gcttgattgg ttttgacttt gtaagttgat aacagaaacg acagcgtctt
    25621 gaactttatc taccgctttg gtgatatcag aatctacatt gactttgaca ttttccacaa
    25681 ctgtttcacc ggcagaattt tgattgccgc ttgtagcagt agaactattg ttgccagtgc
    25741 ccatcgctgc ataaaaaatg ccagctgtca gtagtcctcc aacgatccct ccaacaagcc
    25801 ccagacccaa cttacgccag aggctattat ttttattttt tttcatcttt ggtgtcacat
    25861 tttttctatc catgtgttcc tcctccaatt acattcattt atctactgtt agtatagtct
    25921 tatttgttga ctttagtcta ctttgagact ttgaactttt tatgaaaaac tttaggaaaa
    25981 atcattgttt tcagatgaaa atatttgatt ttctttttcc acagatattg tgtaaaacta
    26041 cgcggatagg attggaaaaa taagggataa aacatatcca cagcctgtgt ataaagtgga
    26101 taaattctta taaaacgtat gtttgcctaa tgaatgtgat gaaataaggt aaaacaacgc
    26161 tgttttctgt ggataaacct gtggatgttg tgcataactg ttggggaaat ctttttaaca
    26221 aaaatagtca aaataaacat atagacgaaa aaattgatta taataaacaa agtgagcgtg
    26281 aagatcaaag gagagaaaac atgaatatca aaattatttc agtaggaaaa ttaaaagaaa
    26341 aatacttgat ccaaggtatc aatgagtatg tcaaacggct gaatgcctat gcgaaaatcg
    26401 aattgatcga agttcctgac gaaaaagccc cggagaattt aagcgaagcg caaatgagac
    26461 aagtaaaaga aaaagaagga gaacggatct tagcgaaaat caaagaacaa gaatacgtct
    26521 atggcttagc aattgaaggg aaaaatccca caagcgaatc ttttgctaaa caaatcgacc
    26581 aattaggtat ccaaggaaaa agccaccttg tttttgttat cggcggttct ttaggactca
    26641 gcgaagccgt catgaaaaga agcaatgctc agatctcttt tggcaaaatg acgtatccac
    26701 atcaattgat gcgcctgatc ctcgtcgagc aaatctaccg tgccttccgg atcaacgcgg
    26761 gggctccata ccacaaataa tgatattttt taagagaaaa aattatccct attttaaata
    26821 atagcaatag gtagtggtag ggagaaattt ataaataact tttttatttg ctttggtagc
    26881 caagtaagtt gcaaaagaaa aatagatact gttaaaaaga taatgtagac atagaaacaa
    26941 atacgtaaaa ttttagttag aataatggca aggagaaaaa acatgataga tgaaaaaaag
    27001 tgtttaaaaa atctattgga ggatatggat gatattgaag aaaaactatc taaatggact
    27061 aggaaaacaa atatatttca gatacttaag ctatctaaaa atgaaataag acatagtaat
    27121 tttttagctt ttttgtttgc tccaaatgaa acccacaatt tatctgatga gttttttaaa
    27181 atgtttttga aaaggtatat agattctaat gatgatacca aagttgctat caattatttt
    27241 gatgctttac ttaatagcta cccgtggtgc tagttaattt taatatccta aagaatat
//
LOCUS       DANJMA010000033        26467 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000033 DANJMA010000000
VERSION     DANJMA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            289..606
                     /locus_tag="PWU31_001683"
     CDS             289..606
                     /locus_tag="PWU31_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL1084228.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="PWU31_001684"
     CDS             complement(697..1062)
                     /locus_tag="PWU31_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084229.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="PWU31_001685"
     CDS             1270..1752
                     /locus_tag="PWU31_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084230.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="PWU31_001686"
     CDS             1949..2839
                     /locus_tag="PWU31_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL1084231.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="PWU31_001687"
     CDS             3165..5639
                     /locus_tag="PWU31_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1084232.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="PWU31_001688"
     CDS             complement(5742..7478)
                     /locus_tag="PWU31_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL1084233.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="PWU31_001689"
     CDS             complement(7475..8179)
                     /locus_tag="PWU31_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084234.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="PWU31_001690"
     CDS             8310..8813
                     /locus_tag="PWU31_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL1084235.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="PWU31_001691"
     CDS             8773..9111
                     /locus_tag="PWU31_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL1084236.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="PWU31_001692"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="PWU31_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL1084237.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="PWU31_001693"
     CDS             10662..11240
                     /locus_tag="PWU31_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL1084238.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="PWU31_001694"
     CDS             11244..11765
                     /locus_tag="PWU31_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1084239.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="PWU31_001695"
     CDS             complement(11844..12398)
                     /locus_tag="PWU31_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1084240.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="PWU31_001696"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="PWU31_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL1084241.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="PWU31_001697"
     CDS             12938..13189
                     /locus_tag="PWU31_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL1084242.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="PWU31_001698"
     CDS             13313..14104
                     /locus_tag="PWU31_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084243.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="PWU31_001699"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="PWU31_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL1084244.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="PWU31_001700"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="PWU31_001700"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL1084245.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="PWU31_001701"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="PWU31_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL1084246.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16161..17297)
                     /locus_tag="PWU31_001702"
     CDS             complement(16161..17297)
                     /locus_tag="PWU31_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1084247.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17414..18106)
                     /locus_tag="PWU31_001703"
     CDS             complement(17414..18106)
                     /locus_tag="PWU31_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084248.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(18244..18897)
                     /locus_tag="PWU31_001704"
     CDS             complement(18244..18897)
                     /locus_tag="PWU31_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084249.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18947..19375)
                     /locus_tag="PWU31_001705"
     CDS             complement(18947..19375)
                     /locus_tag="PWU31_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL1084250.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(19380..19754)
                     /locus_tag="PWU31_001706"
     CDS             complement(19380..19754)
                     /locus_tag="PWU31_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084251.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            20039..20224
                     /locus_tag="PWU31_001707"
     CDS             20039..20224
                     /locus_tag="PWU31_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084252.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            20237..20485
                     /locus_tag="PWU31_001708"
     CDS             20237..20485
                     /locus_tag="PWU31_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084253.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            20516..20695
                     /locus_tag="PWU31_001709"
     CDS             20516..20695
                     /locus_tag="PWU31_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084254.1"
                     /translation="MGKFNRALVFSTPFIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(20738..21208)
                     /locus_tag="PWU31_001710"
     CDS             complement(20738..21208)
                     /locus_tag="PWU31_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084255.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            21295..21993
                     /locus_tag="PWU31_001711"
     CDS             21295..21993
                     /locus_tag="PWU31_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL1084256.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22171..22512
                     /locus_tag="PWU31_001712"
     CDS             22171..22512
                     /locus_tag="PWU31_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084257.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            22505..23176
                     /locus_tag="PWU31_001713"
     CDS             22505..23176
                     /locus_tag="PWU31_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL1084258.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            23182..23868
                     /locus_tag="PWU31_001714"
     CDS             23182..23868
                     /locus_tag="PWU31_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084259.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            23871..24701
                     /locus_tag="PWU31_001715"
     CDS             23871..24701
                     /locus_tag="PWU31_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL1084260.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            24718..25569
                     /locus_tag="PWU31_001716"
     CDS             24718..25569
                     /locus_tag="PWU31_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1084261.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            25566..25925
                     /locus_tag="PWU31_001717"
     CDS             25566..25925
                     /locus_tag="PWU31_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL1084262.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            25940..26101
                     /locus_tag="PWU31_001718"
     CDS             25940..26101
                     /locus_tag="PWU31_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1084263.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            26098..26403
                     /locus_tag="PWU31_001719"
     CDS             26098..26403
                     /locus_tag="PWU31_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084264.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT         8896 a         4504 c         5264 g         7803 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 tattatctgt ttttttatat taacgatttc tatttggtac atttttgatt aataaaaagg
    16141 ggcaaaaaag gggcaacaag ttacagtttt tctatatctt ctttcaattt ttcttttact
    16201 ccttttgtca catgcaaata aatcttttca gtgatatcac tattttcgtg cccaacgcga
    16261 tcctgaatag catacagagg agttcctagt tccgctaatt ttgaaatatg ggtatgtcta
    16321 aaaatatgtg aactaagttt tttatcaatc ttcatgtcag ctttatgatt tctcaaatag
    16381 gtgttaattg ctgttagttg gaaaggagtt cctttcgttg tttgaaatag aaattgaccc
    16441 ttcggattta attctagaag ctcattgtaa atagctatcg ctttttttgg taaatcaatt
    16501 tctcgcattc cagcagcagt ttttgtagaa tcagattttt tcatatcagc tattgaacgt
    16561 tctctataca tcatcgtccc atttataact actgatgcat tattattggt aatgtgtaca
    16621 tcgtctttac ttaatgcgat tgcttctcca ggtctcattc cagtcaaata tagccactgg
    16681 aaaaggagag aatatctttt attgtgtgaa gttgtgaaat ctactagtct tttatattca
    16741 tcatcttcta aaaacttatc tttaattttt atcgtttttg actctcgttt ataatcaata
    16801 actacttcgt tgataggatt tttttctaca tagccttttt tcatagcgta tgaaaaaaga
    16861 agattcaatt tggatttaat tacactgacg tacttatttg acaagtcatc tttatatatc
    16921 atatcttcaa aaatattatt taaatctact gtattaatac cagaaacgat gtaagtctca
    16981 ggcatcttct tctttatagt gtttaagata ttattcgtag gataatatgt agactcctta
    17041 acttgccttt tgtaaataac taaccattct tcgacaagct catgaaatgt tagattaggc
    17101 ttctggagtg ttttttcatt tagttttata tcaatttttt tatttaactc cagtaatgcc
    17161 actttctgag tttctcgaga cttattttta taagtaatgc tcactttttt tctttttcta
    17221 gtttttggat caatatatcg ttcattatat ttatatactt tttttccgtt tttatcagtt
    17281 ttggtttcaa tccacattgt tttcatctcc tatttgttgc tataatagga atggataagt
    17341 aagccgatta tagcaggttt atttttcatc acgtccacaa actttggcga gggagggggc
    17401 gtgtttttgt attttaacga tttatattga atgttgattc agcactttca gcaactgtac
    17461 tataatcaga cggattatat aacactccgg tttttatcgt tacttttgta atatcgttaa
    17521 ctttagatgg atctaagtaa gctaaactat aaaattcttt actttcagtt tttttgaaaa
    17581 acgagtttgt tgtcattgta tgcataagat cgtctgaact gaaatcaatt tgttggccgt
    17641 tatcaagtat ggcgtgtgca agcccattat aaccataatc tacttcagaa ttgtttttaa
    17701 ctttgaatgc aatttgtaaa ccataaaaat ttccttcact atctttgtga ttatttgcat
    17761 cgtagtaacc agcagcgcta gggtcagcat aattggaata cttgagtagt ttgatattag
    17821 atatataaat gccgatatca ccaagaggaa tatctgattg tgggcttgcc gttccaatta
    17881 attcaatttt gccacttgta tctctttttt ctttggtcca ttgaccaact ttgaggaggg
    17941 ggccatcaac ctcctgtgaa tttttagaag actgtgttgt actttctgat aacgtaactg
    18001 aagagtcttt ttcgtttgtt ttgccagtat tatctacaac attagattgg ctacatccgg
    18061 taagaaaaaa agcaccaaat actaacccta aaatcacttt tttcattctt tattctccgt
    18121 ttctatgata ttatttttat gtaggatctt agaaacgaga tcttagtccg tgttgcagca
    18181 cgggcttttt tctttataac ttttttagag attataggca aaatagtagg acacaaaaat
    18241 atattattgt attccgtatt tagaaaatcc taattgaact tcaccagaag ttttttgctg
    18301 tgttgtacgc aatgcttctt cttcagacat tccattctgt actttccatg caacaggcga
    18361 catcccgtat ttgttaacaa aatcagtaag tgataaagtg tcagcgtctt gctgaccgct
    18421 tgtttgttgg tcttctggat tttgttgaga tgctgcttgt tgttctttct gatcttgact
    18481 gataatattg ccagcatcat ctgtagtcaa tccattttca taaagggcca cgccgaaagc
    18541 ttcccactct ttgttggacc aatttgcacg atcagctgga gttgactgta aagtgcgttg
    18601 tttcatctgt tcatatgttt cttcttgagg tgcggtttgg attgtatcct gactggagct
    18661 tataactgtt gggctaggtt ccgccgtagc ttggttagag cttgtaactg ttgaactaga
    18721 atctgtctta gatgtagatt tgctagtaga ggaactggtt tcagttgttt cttttgtttt
    18781 acttactttt gtttcttggt tagaagtggc atctgttgat tcagcttttt tattatttga
    18841 acaagctgaa agtagcagag cagtacttaa caacaacata acgctaactt ttttcatttt
    18901 ataattcctc tttctcgttg taatatgtgt gctaacacgg gcttttttat ataagaaaac
    18961 gataagcgct ttctggaagt ccgtaaagat tctttaattc atcgattttt ttgggatatt
    19021 gatcattatc ttctttataa agagaaacaa tgagattagc agcaaagcaa ttagcttcgc
    19081 tttcagattt gcttctagat gttcttgttg atacatagta actggataag ccgcgatgaa
    19141 aaatagcgtg acctaattcg tgagcgcaaa tgtagaatct ttcctcagag tctcgcagtt
    19201 catcatttaa gaagattatt gcacgacctc taatttcttg aaactgtcct ttgggatttt
    19261 cgataaaagg aacgtattga attttaatgc ccatcttttc acaaatataa aaaggattag
    19321 cagaccggta tttccgcttc aactcctcga ctaaattaat cgtatccatc tccataagct
    19381 cacatctttt tgcctttttc tttgtcttct ttcacaatat cccagaaggt tccaataagg
    19441 atatctttta cgcgttggac ctgttcaggc gtcagtgttt ctccgccata agacatattg
    19501 acatttgaat ctagtaattt atcaagttca actacttcat cttttgttgc ccagtcagga
    19561 acttggtttc taccgagtaa ataatcagta gttacattaa aataatctgc tacaagttgt
    19621 agtcttttag tactaggagt tctatttttc cattgataaa tcgtgttttt aggtatattt
    19681 aattcttctt ccaattgagt tatacttatg cctcttttat gagctaattc ctttattcta
    19741 tctagtaaat tcattttcgc acctcacaag ctacgaaaac actaataaaa aaattagtta
    19801 aaggtgttga caactaataa aattgttagt atactgtttt cgtaagctaa aatattagct
    19861 aaaaagactt gataactaat aaacacttca cggtcggcaa actctgaaat gtaaattact
    19921 aggcatttct gtgtcttatt tagctatatc tatatactaa taaaaatatt agttattgtc
    19981 aacgaatatt agctaatttt ttagcttaca gactattttt tagaaaggag ctatttttat
    20041 gtctgagaat ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa
    20101 agaactagct gcgcttgttg gtatttcagg agcttattta tcagatattc taaacggcaa
    20161 tcgtgatgga aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg
    20221 ataggggggg taggcgatga caaaactaaa aaaacaagat tttgtaaaaa aatacaatta
    20281 ttctccatct acttatcaac gtcgaatgtc ggaactaaaa aatacagcaa ttttctcagc
    20341 ggcgtatgaa cgggtcacag gacaagaagt ttggatcaat acagaattat acgataaatt
    20401 tttgtctttc aaatcctata acaggttacg cacaagaaag ataacaccta aagaatttat
    20461 cgagaagcat ttagttgatt tataaaaaac agaacatttt gagaggtgaa ggttaatggg
    20521 caaattcaac agagcactag tattcagcac accattcatc atctacgctt taggcctctg
    20581 gggaagcagg caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct
    20641 tgatgaagct gaagctgagt acagagcgaa aaagccaacc gagggggctg actaaaaatg
    20701 aatagaaaag agaaactaga atgtatatta ctattactca gtttaattct ttcactaatt
    20761 tctctattgg gaagtttgta tttttgatat caacaatttt gtggatttgg gttctattta
    20821 gagtaaatat catttttgtt tggtgattaa ggagtttatt aaaattaatt ttccctactt
    20881 caaatgcaag gtaacaagat atagcacttt taggaggaat atttagcggt acagcttcag
    20941 agtatattct attatcatcg actgatcctc ctttagcaat taatttctta aatggagtag
    21001 ctgaccatct gcggtttaag ttagcggatg aaagttctaa attaactaac atagctggtt
    21061 ctgttgaata gttagaaatg attacttttg tataaatcat atctgcagcg aagtaagaag
    21121 cattaagttc aacttctagt tgagggcgca ttccttttat ttttatccct gttaaaaacg
    21181 tactcaatat aaaacttatt attgacatcc attgaaaaat tgttaagtga aaaaaattca
    21241 aaataaacac caccagtttt taactaaatt ataccaaaaa ggagagaaga aataatgcaa
    21301 gaattagtaa ttttgaaaaa taaagaagct gtgactacga gcttacaagt ggcagaaagc
    21361 ttcgagaaaa aacatcaaca tgttttaaga gatattgatg cactaaaaaa agatgtgtcc
    21421 aattttggac agatgtttgt agaaggtaat gaaccagatt catatggcag aaatcgacga
    21481 gttttcttca ttagtagaga tggatttttc ttgctggcta tggggtttac aggaaagaaa
    21541 gctatctact tcaaacaaaa atacattgaa gcattcaacg aaatggaaga tgttattcgc
    21601 aagaatactg ttcctcaaac aattgaagat atgatgatct atcaattaga agaaatgaaa
    21661 gatgttaaaa aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag
    21721 tttgaaatta agcaaaaagg aaatatgaaa gttatgggag ttctaggggg aaaagaaagc
    21781 cgagcttatg aagaaatcag caaaaaagta ttctcaaaat tttggtctga atttaaacgt
    21841 accttttcaa tcccaagata tggcgagtta cctcgtaaga gattcgatga tgctgtttca
    21901 tttattgaaa tgtggttgcc agaaactgcg atccgtatgg aaattgatca actgaacaga
    21961 caacagagac tttttggtga tgacaatgaa tagagctgaa gcgctaagaa tagggacggc
    22021 aattgctaat tgctggtgga aatactataa accaatcatc ctaagccaac aacatattga
    22081 caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa
    22141 agaaaacaca tcataaggag attttagcat atggaaaaag aactttccac tctagatcaa
    22201 tatttgattg atcctgattg gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc
    22261 agacgaaatc ttttgacgaa tgaagaacta gcttgggatc aagatgattt aggcaaccat
    22321 gtaactattt gggatcatgt ttatcttatc catctatcga agcattcgaa taaacctgaa
    22381 tatatttacg tcatcgaaga tggcttgatt gatgcgctag aagagtacga cagagataac
    22441 ttgattgata tctcttatta cggaccaggt aagaaataca ttgctgaaat ggaggcagaa
    22501 tttgatgagt gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa
    22561 taaaattctc aagaaaaaag gaaattttga ttatttatct tgggctcacg cgtgggagat
    22621 tatgaaaaag aatgatccac aggcaacggt aactattaat gagtacaaac actacagggt
    22681 tgtttctgga actcatcaag actttcttgt tgaggaatat aaaccttttc ttatggatga
    22741 aactgggact tatgtatctg tctcagtaac ggttaaagga cacacggaaa ccgaattatt
    22801 tcctgtttta gattatcgaa accaaccagt tgttaaacca aatgcaatgc aaatcaataa
    22861 ctcattgaag cgatgctttg tgaaagcatt ggctctacac ggactgggat tatatgtatt
    22921 tcaaggggaa gatattccaa caccacctag aatcgataca aagaaattaa acatgctaga
    22981 gacgattcta gaagctttca atgagcagat gggtaaagat atgaccaaaa ccttaatcga
    23041 atatgttaat gagcagacag ataaattagg gctcttagct gataacgttg aaactattga
    23101 acagttaagc tatgagcaat gtgccttgat ggagcgagca atagcagcta agagaaaaga
    23161 attagataag aagtgatatg agtgtttaaa ccattaatcg attcatattc agcggttctg
    23221 aaaaagttca aaggaaaaga cataagcgca accatcaatg aggaagtgaa cattgatcga
    23281 ctaaagacga tgtatgacgg ctacgatggt gatcgagtta ttgaaattcg ttttattgat
    23341 cctagacgtt tcacagtaca gcaacgaaac ttcatctatg cactcatagg cgatattttc
    23401 atcgatacag gcatgccaac ggacttctgg aaggaattct tttacttccg tttcgaaggt
    23461 gtcacagggc gcgaaataag cctcaaagat gaatcgaata cgactgtgag tgatgccaat
    23521 atcttagcaa atatcatctt agattttatc tttgaacatc atattccttt caaagaaggc
    23581 tatgagattt taccagcgaa tcaagagtat tacttctaca aatgcattac aaaaagagtc
    23641 tgctgcatct gtggcaaaac aggagctgat atcgatcact ttgacaaagc gctgggaaga
    23701 cgaaagcgca aagaagttga tcattcagag tacacatttg cagcactctg cagaatccat
    23761 cacacagaga aacacaaaat aggtgtgatc aatttcaaaa ataaatatca aatcaaaggg
    23821 atcaagttaa accaggaaac aattaaaaag ttaaggatag gaggataaat ttggctgaga
    23881 taagttggat caaacttaaa actactatgt ttgacgatga aaaaatacga ttaatccaag
    23941 ctgttcctga gtcggatgcc atcatcgtta tatggattcg attactagtt ttagcaggaa
    24001 agactaacga cgatggtctg atatatatcc agaggaacat gccttatacc gaagaaatgc
    24061 ttgctacatt gtttggcaaa aacgtaaata cggttcgctt agcgctaact acattggcaa
    24121 atttcaacat gattgatcta agcagtgatg gactaattgc catcagtaat tgggaaaaac
    24181 atcaaaatat cgagggtatg gataaagtaa ggctaaaaaa tgctgaaaga aaccgtaaat
    24241 acagggaaag aaagagacag gaacgtctca aattggaaaa tgacgttagc gtgacgtcac
    24301 gtgacggtac agataaagat atagaagaag ataaagatat agataaagaa gaaaagaaag
    24361 gtaagtattc tgacgaacac ttacgccttg ctaaaaagtt gcaaagtaat ttaactgaag
    24421 attttccaaa agaaatgaac aaagtagata tcgaaaaatg ggcagacaca atcaggttga
    24481 tggaagaaag agataaagca tctatagaag cgattgagta tgtgatcaat tggctaccta
    24541 caaatgaatt ttggtttgga aatattagaa gtgctaagaa attgagagaa aaatttgaga
    24601 agctcaaatt cgaaatcaaa gcagacaaga agaatcataa aaagcaaagt caaaaactac
    24661 agtacagcaa tcctagtgaa tatgacgact tgccaattta aaaaggagat gcatcacatg
    24721 gaaagcctag caaatgctat ggagaaacta ataagaagag tattagtgca aagcggaaaa
    24781 tgtccagaat gtagcgaacc tttgtatagt tggcgagcta aaaataagga tggttcagaa
    24841 cgttgtaaac caacatgcat gagttgtggt tataaagcgt tacgtgtgaa agaggatata
    24901 cagaccgaac ggatatataa cgatagctta aaagcacgag cgttgagttt ttttcaaaat
    24961 ggttcggtat taacagataa aactttgttt aaatgcaaaa tggaaaatta tcacgtagtg
    25021 gatcaagaaa caaaaatcgc tttagaaaaa gcaaaaagct atacgaatga ggttctgctg
    25081 aaccatcctg cacatttcat tctatcaggg aaatcaggaa gcggaaaaag ccacttatca
    25141 atggccacag cttgggaaat acttgagcgc tcaaattatg acaagaaaat actttttata
    25201 agctatcaag aattattaga gcaaataaag ttttcttata acaatactga actgagaaaa
    25261 gaaattgaag gatcgcttat agccgatatt aaaacaactg atttggtggt ttttgacgat
    25321 attggagctg aattaggtag tggggtatca aatagtaggc agtttacaaa caacacgtta
    25381 aacacgctct tagaagccag acagaacaag gcaacgatca tcacaacaaa cttatctggt
    25441 cctgaactaa gagaagccta cggtgaaaga attgtttcta ggatatttaa gaattcagaa
    25501 ggttatgcgc tgaaattcca acaaacagca gacaagcgca taaaaccagt gaaaggtagt
    25561 atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg aggtatcaag tttgattcta
    25621 tcgcagaagc agagtattac gatctagcct tgtggcaagc tgaagcaaat ggctggaaag
    25681 taaaacttca ggaaagattt gagctgatgc cgaaatttga actagacgga aagaagtatc
    25741 gcaagatcga gtatattccc gacttcacat tttataaaaa cggcaaactt gtcaaagtcg
    25801 tagatgtcaa aggaatgcag acaaaagact ttaagatcaa ggcaaagttg ttctgtcatc
    25861 aatatcaagc gccgttgata ttagctaaaa aatatcggaa tacgttcaag gaagagcgtt
    25921 tttaacgagg tggtccatca tgacaacaga agaagtgatt caaatgcgta ttcgaaacat
    25981 tcagcgtgaa attgacgatc tagaacgaac aaaggcagtg atggtcaatg aaacggcgag
    26041 aaaggcaatc gatttgcaca tagagaactt aagaagggaa attcgtagat tggaggaatg
    26101 agcgtggata agaaagcgac aatgaaacga attgctgaat taaccaagtc agaatcttgg
    26161 caggaagaca aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa
    26221 aagcctaaac ggaaaacgcc gagaaaaatt gcaatctggc atggtgatcg aattctagta
    26281 acaggtacag ctgaacagtt atctgaaatt actggattaa gcaaaaacat tatctgggat
    26341 agagctagga gcttatggat tgattcaaaa ggacgacagt ttaggtatgt ggaggagaga
    26401 taatggatct cattacacaa tacagtgaca tcatcctcaa gaaaatcatg atgaagattc
    26461 agaaaga
//
LOCUS       DANJMA010000034        25344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000034 DANJMA010000000
VERSION     DANJMA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            12..84
                     /locus_tag="PWU31_001720"
     tRNA            12..84
                     /locus_tag="PWU31_001720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="PWU31_001721"
     tRNA            88..160
                     /locus_tag="PWU31_001721"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="PWU31_001722"
     tRNA            168..249
                     /locus_tag="PWU31_001722"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="PWU31_001723"
     tRNA            267..339
                     /locus_tag="PWU31_001723"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="PWU31_001724"
     tRNA            351..422
                     /locus_tag="PWU31_001724"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="PWU31_001725"
     tRNA            439..524
                     /locus_tag="PWU31_001725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="PWU31_001726"
     tRNA            535..608
                     /locus_tag="PWU31_001726"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="PWU31_001727"
     tRNA            618..691
                     /locus_tag="PWU31_001727"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="PWU31_001728"
     tRNA            711..783
                     /locus_tag="PWU31_001728"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="PWU31_001729"
     tRNA            811..884
                     /locus_tag="PWU31_001729"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="PWU31_001730"
     tRNA            898..971
                     /locus_tag="PWU31_001730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="PWU31_001731"
     tRNA            995..1084
                     /locus_tag="PWU31_001731"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="PWU31_001732"
     tRNA            1097..1170
                     /locus_tag="PWU31_001732"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="PWU31_001733"
     tRNA            1189..1264
                     /locus_tag="PWU31_001733"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="PWU31_001734"
     tRNA            1266..1336
                     /locus_tag="PWU31_001734"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="PWU31_001735"
     tRNA            1350..1423
                     /locus_tag="PWU31_001735"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="PWU31_001736"
     tRNA            1448..1535
                     /locus_tag="PWU31_001736"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="PWU31_001737"
     tRNA            1557..1628
                     /locus_tag="PWU31_001737"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="PWU31_001738"
     tRNA            1651..1740
                     /locus_tag="PWU31_001738"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="PWU31_001739"
     tRNA            1753..1826
                     /locus_tag="PWU31_001739"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="PWU31_001740"
     tRNA            1845..1917
                     /locus_tag="PWU31_001740"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="PWU31_001741"
     tRNA            1921..1993
                     /locus_tag="PWU31_001741"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="PWU31_001742"
     tRNA            1998..2068
                     /locus_tag="PWU31_001742"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="PWU31_001743"
     tRNA            2081..2154
                     /locus_tag="PWU31_001743"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="PWU31_001744"
     tRNA            2166..2237
                     /locus_tag="PWU31_001744"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="PWU31_001745"
     CDS             2503..3696
                     /locus_tag="PWU31_001745"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL1084265.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="PWU31_001746"
     CDS             3826..5298
                     /locus_tag="PWU31_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1084266.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="PWU31_001747"
     CDS             5381..6169
                     /locus_tag="PWU31_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL1084267.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="PWU31_001748"
     CDS             complement(6236..6904)
                     /locus_tag="PWU31_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL1084268.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="PWU31_001749"
     CDS             7019..8731
                     /locus_tag="PWU31_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084269.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="PWU31_001750"
     CDS             8734..10503
                     /locus_tag="PWU31_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084270.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="PWU31_001751"
     CDS             complement(10708..11274)
                     /locus_tag="PWU31_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1084271.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="PWU31_001752"
     CDS             complement(11274..12230)
                     /locus_tag="PWU31_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL1084272.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="PWU31_001753"
     CDS             complement(12230..12886)
                     /locus_tag="PWU31_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL1084273.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="PWU31_001754"
     CDS             13256..15274
                     /locus_tag="PWU31_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1084274.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="PWU31_001755"
     CDS             15701..18115
                     /locus_tag="PWU31_001755"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL1084275.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="PWU31_001756"
     CDS             18463..20016
                     /locus_tag="PWU31_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL1084276.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="PWU31_001757"
     CDS             complement(20397..22181)
                     /locus_tag="PWU31_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084277.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="PWU31_001758"
     CDS             complement(22196..23917)
                     /locus_tag="PWU31_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084278.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="PWU31_001759"
     CDS             24017..24778
                     /locus_tag="PWU31_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1084279.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7843 a         4651 c         5384 g         7466 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttagacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcgaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    25321 aaaaatcaca aaggttattg ttat
//
LOCUS       DANJMA010000035        25286 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000035 DANJMA010000000
VERSION     DANJMA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..489
                     /locus_tag="PWU31_001760"
     CDS             <1..489
                     /locus_tag="PWU31_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1084280.1"
                     /translation="RTSNPERDSFLFLFGINNGLRMSDIIGLKVGDIRGKSKPIIVEH
                     KTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRHITRDRVYQIYADIAEKLGR
                     DDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITKRYIGITEDEIGASLRGFKL
                     GV"
     gene            complement(662..1135)
                     /locus_tag="PWU31_001761"
     CDS             complement(662..1135)
                     /locus_tag="PWU31_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1084281.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1147..1683
                     /locus_tag="PWU31_001762"
     CDS             1147..1683
                     /locus_tag="PWU31_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1084282.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1723..2094
                     /locus_tag="PWU31_001763"
     CDS             1723..2094
                     /locus_tag="PWU31_001763"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1084283.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2378..3649
                     /locus_tag="PWU31_001764"
     CDS             2378..3649
                     /locus_tag="PWU31_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1084284.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3894..4919
                     /locus_tag="PWU31_001765"
     CDS             3894..4919
                     /locus_tag="PWU31_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084285.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5213..6322
                     /locus_tag="PWU31_001766"
     CDS             5213..6322
                     /locus_tag="PWU31_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1084286.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6405..7301
                     /locus_tag="PWU31_001767"
                     /pseudo
     CDS             6405..7301
                     /locus_tag="PWU31_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7317..8141
                     /locus_tag="PWU31_001768"
     CDS             7317..8141
                     /locus_tag="PWU31_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084287.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8173..9483
                     /locus_tag="PWU31_001769"
     CDS             8173..9483
                     /locus_tag="PWU31_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL1084288.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9631..10890
                     /locus_tag="PWU31_001770"
     CDS             9631..10890
                     /locus_tag="PWU31_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1084289.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10900..11772
                     /locus_tag="PWU31_001771"
     CDS             10900..11772
                     /locus_tag="PWU31_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084290.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNTNIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11784..12614
                     /locus_tag="PWU31_001772"
     CDS             11784..12614
                     /locus_tag="PWU31_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084291.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12654..14837
                     /locus_tag="PWU31_001773"
     CDS             12654..14837
                     /locus_tag="PWU31_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1084292.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14898..16118
                     /locus_tag="PWU31_001774"
     CDS             14898..16118
                     /locus_tag="PWU31_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL1084293.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16115..17575
                     /gene="gtfA"
                     /locus_tag="PWU31_001775"
     CDS             16115..17575
                     /gene="gtfA"
                     /locus_tag="PWU31_001775"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL1084294.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17652..18758
                     /locus_tag="PWU31_001776"
                     /pseudo
     CDS             17652..18758
                     /locus_tag="PWU31_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19080..19289
                     /locus_tag="PWU31_001777"
     CDS             19080..19289
                     /locus_tag="PWU31_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1084295.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19282..19431
                     /locus_tag="PWU31_001778"
     CDS             19282..19431
                     /locus_tag="PWU31_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL1084296.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19585..20250
                     /locus_tag="PWU31_001779"
     CDS             19585..20250
                     /locus_tag="PWU31_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1084297.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20534..21619)
                     /gene="xerS"
                     /locus_tag="PWU31_001780"
     CDS             complement(20534..21619)
                     /gene="xerS"
                     /locus_tag="PWU31_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL1084298.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            21953..22186
                     /locus_tag="PWU31_001781"
     CDS             21953..22186
                     /locus_tag="PWU31_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL1084299.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22183..22590
                     /locus_tag="PWU31_001782"
     CDS             22183..22590
                     /locus_tag="PWU31_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL1084300.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23727..24245)
                     /locus_tag="PWU31_001783"
     CDS             complement(23727..24245)
                     /locus_tag="PWU31_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL1084301.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8591 a         3875 c         4724 g         8096 t
ORIGIN      
        1 agaacaagta atccggaaag agatagtttt ttatttttgt ttggaatcaa taatgggtta
       61 cgtatgtcag atattatagg attaaaagtt ggagatatac gaggcaaaag taagcctatt
      121 attgttgaac ataaaacagg taagcgaaaa ccgattttta tagataattt gagagaagaa
      181 attttattat atactgaagg taaagaagaa aatgattggt tatttccgag tcggcaacaa
      241 ggtcgacata taacaaggga tcgtgtctat cagatatatg cagatatagc agaaaaatta
      301 ggacgagatg atataggaac acatacatta agaaaaactt ttgggtatca ctactacaag
      361 aaaactagag acattgctac cttaatgttt atttttaatc atagctcgca agcaattaca
      421 aaaaggtaca ttggtataac tgaagatgaa ataggagcat cacttcgtgg atttaaacta
      481 ggtgtataat ttaaaaattt ttataaacag taaagacaaa gattaaaagc tatttttatc
      541 tagaacgatt ttatttcata aagttcgcgg aataaaagct ggggttactg gtacttggta
      601 taggttcgtg ttatataaac taagtaatta aataacattt ttaggttgga aaaggatgaa
      661 attaaaaaaa gagaatcaat acggtctcaa aaacgcaaac gctgcggaat aagaaaaata
      721 ctgataactc aacgtttagc agactatcta atagggatag tctgcttttt tcgtttttga
      781 ccatactttt gaccttctgc aaacttcctt gcttttcctc tgtagaactc atgaaatctg
      841 caaaacgaat tgccgtttcc tctgtctgct ttttcgcgat atgggtgtat atatcatcca
      901 tagtggtttt aatatctgta tgtccaagtc gaacatacac ttctttgaca gacgcaccag
      961 attcaaagag cagactacaa tgggtatgac gaaaaccatg aagtgtgata tgttttaagt
     1021 tattctcttt aatgatgatt ttcaaattgt gatttacata gtctaaataa aggggcttat
     1081 tttcttctga tgaaaacaaa agttgttcac catcttttat aggtttgatt ctcattttta
     1141 gatattatgc ccgcacgtat tcatcacatt tctattgtga atcgctttat tcgaccgaca
     1201 tttgactttt atcataatat actcggttta aaattactaa tgaaaacaat taatcaagac
     1261 gatcatacca tgtatcacct gtttttttca gataatcacc accgtgtggg aactgaactg
     1321 actttttttg aagtacaaga agggaacaac cagttttttg gaaccaatac gattgaacga
     1381 acgatactaa aagttccttc cgaagcatca ttgcattttt gggaaatata ttttgaaaca
     1441 caaggcgttt gccattatgg aatcgaatca ttttctggac gacctatctt acgttttgaa
     1501 gggccagatg caacacgatt agctttagtt cccttacgcg aatttgaaga tattgatgat
     1561 tatttcccgt atgtcacaga ccagatccct acagagcatg ccattttagg aattgatgcc
     1621 attcaactac gcgtacaata ttccgatgcg actgaacgag agttattgtc ggtaggctgg
     1681 tagcacaaag aatacgtcag tttcttttcg ggtcatcaac gagtgacaat cttagaaaat
     1741 caagacactc agttctatca agaagttcag attattcaag atcgtgagaa tccagttgcc
     1801 attgaaggga ttggtagcgt ccatcatgtt gcgtttggcg tagaaaataa atctgattta
     1861 caacgcatcg acaaacaatt acaagagcgc aattttataa attcaggaat aaaagatcgt
     1921 gaattttttg tatcacttta ttatcgtgac ccaaatcaat tattgattga aattgctaca
     1981 ggggaaggaa acctagatgc aaaagcgtat gaaaatcaaa gtccacgctt tgaagaaatt
     2041 cctttatttt taccgcagta cttaaatttc attcgtgaac aagtggagca ataattagct
     2101 aatcaaatcc atgacgaaga gtaagaggtt gactcataag tcaaaaaata aagaaaaacg
     2161 catcaaatcc attctaaaga atgttgattt gatgcggttc ctatttttat aaactatcat
     2221 tttcgactaa taaaactact tatgagtcag cctctctttt tttcttgatt tttatacctc
     2281 tttctaggta aactaaacat ctgccaaaaa taccgtgtct cattgaaaca ttctcaacta
     2341 tttcaaaata aaggggagta ccaaatgaac gagtatattg aaattatcca cgcaagagaa
     2401 acaacttaaa aatgtcagtc tcaaaattcc caaaaataaa attagtattt ttaccagcgt
     2461 atctggttca ggaaaatcat cgattgtttt tgatactatt acccaagaag ctggtcgtca
     2521 actgaatgac acctatgata gttttacccg tttattttta cctaaataca gtcgtccaga
     2581 ggttgatgag attgataatc tttctacggc gattgtggtc aatcaaaaga agattggtgg
     2641 cagtgcgcgc tcaacgttag ggacgattac taacatcaat gcgttatttc gtgtgttgtt
     2701 ttcatgtttt gcaacaccta gtttaggata tgcaaatgct tattcattta atgatcctag
     2761 tggaatgtgt tctgtctgtc agggaatcgg taagacgatt gcatttttaa taggattgct
     2821 ttatttttcg gctattgata atgctgattt tgtattttct gatccattta ccgcagaagt
     2881 tttgtctgga attgaggaac aggcaaagaa tcatggttat tttgtattgg tgcataatgt
     2941 taattcagca gaagatgtta aaactattca gagaaattgg cgttttgagg ggttcattgt
     3001 tgttggtgta cgtgtaaaag aatttttgga attaaataac gtttttgatg gtcctgttgt
     3061 ttatattgat acgcatcttt caaaagagac attaagagaa cttgaacaac aaatgaatcg
     3121 tttatttgta aatacaaatg attaccaagc tagtacattg gcagtggaac atttgattca
     3181 ttatggtcat gagaaaatag cgtttctttc ttttgactac attgtaggtg aaccaagtgt
     3241 tattcaagaa cgacatcaag cttatatcga tacattaaag aaatatcatt taacctttaa
     3301 atcgcattgg atatataaca actctgaatt tgagcgaatt tatcaagaat tagctgaatt
     3361 tactgcaatt gttgttactg gagattattt agcggctaaa ttagtaaaat ttttaaaaaa
     3421 taagaataaa cgagtgatgg aacaattatc gattattgga tttgatgata ttacttttgc
     3481 agaattaatg gatcctgcgt tgacaacaat acgactaaat ccaggtaaca agggggcgat
     3541 tgcttttcac caactgattg gactggccaa acaagaagaa ataaaaaatc aagtactact
     3601 attagaggga gaattgattt ctcgagactc tgttcacaaa atcaaataaa aaagctctgt
     3661 gaaataagta tagttaacaa taaaatatcc gaacgataga gagaatgaac tgtgatctag
     3721 cagttcctcg tcgcaaattc actagaatta gcattattta ctctaaattg ttcggatatt
     3781 ttttcttgtt tttaattatg attacagtgc ctatttttaa cgaaatttgc ttattttcaa
     3841 aatgtatgat tatatgttag taaacacttt caaatatgaa aagaggagct attatggtag
     3901 gaattaggga tgttgcaaaa cgggcgggtg tgtccattgc aacagtatcg cgcgtgctaa
     3961 atgaggatcc gacactttca gtaaccgatg aaaccaaacg aaagattgca gaatcggtta
     4021 atttttatca ttatgtcaaa aaaagtcccg ttgcaaaaaa tttaacaaat attgcattaa
     4081 taacgacagt ttctgaagta gatgagttgg aagatcccta ttttcgaacg attcgcagag
     4141 ggattcaagt cgaagcagaa caaagcaaag tcaatttgaa gaaaattatt cgtttgtcag
     4201 aaacaccgtt ggagcaagac gatttgctaa aatgtcaagg tgtgttgatt attgggcagg
     4261 tgctatcttc tgtgattgaa gaagtatcag caattaattc gaatgtcgta gtgattgatg
     4321 accctagtat tgaagctgaa gtagatgcag tttatactga tttaacaaaa gcaacgaatc
     4381 agcatttaga tcgattgtat aaaaaaggac accgaaacat tgcgtttatc ggaggaggac
     4441 gtgttcgttt agatagccaa ggaaataaac acgtgtcaga tgatgattat cgaaaaatag
     4501 cctatgagac gtggatgaaa caaaaaggat tagaaaaata tagtcaaatt tatttagaag
     4561 gctggaccac acttgacgga atgcaggcag cagatttact tttgaaaaaa catgctaaag
     4621 ataagttgcc gactgctatc atggtaggaa atgatcctat tgctgtgggg gtttatcgtt
     4681 cacttcaaaa aaatggtcta catattccag aagatatttc gattgtgagt tttgataata
     4741 ttgaagttgc tgaattttta acacctgcat taaatacggt gaatgtgaat acagaagaaa
     4801 ttggaaaact agcagttcgc atggttaatg aacgaataaa agaagttaga aatgtttcga
     4861 tcaaagtgat tgtttctaat aatattattg ttagagaaag tgaaaaaaat atcgattaaa
     4921 aaagtaaaca tccaaaaaag ggtgtttact tttttgttta ctaatatttt tagtaaaaaa
     4981 tctcgacaaa acattaagta aacagttatg gtgttgttaa caaaaagaaa acgcattcaa
     5041 aacaggagga attaagatga aacatttcaa aaaaatagct ttaacaggga tagctgtttt
     5101 aactgggaca ttactcgcag catgtggtgg gggagatagt aattctaaag acgggcaagt
     5161 aactttaagc tatgcgagtt gagatactga ccaagctcca ggactaagaa aaatgttaga
     5221 tgaatttgaa aaacaaaatc ctgatatcaa agtagaaatg gagacaacgc cttgggatca
     5281 atattgggtt aaattagaag ctgcaacaac gggaggtaat atgccagatg tagtaaccat
     5341 gcactctagc gaatcctata aatatatgtc acagaatgct ttgatgggac ttgatgatgt
     5401 tatcaaagaa aatgatattg acatgggaaa ctttactgaa ggaattgctg atttctatac
     5461 gcttgaagac gaactatacg caatccctaa agatgcttct gtagtggggc tatggtacaa
     5521 caaagaatta tttgataatg caggtgtcga ttatccagat gaaacatgga catgggaaac
     5581 actcagacaa gctgcaattg atttaacaga tgaatcaaaa ggcatctatg gttttgcatc
     5641 acatattgga acagagactg gctactggcc atttatttat caaaatgggg gagaagtatt
     5701 tgccgatgac aatacaaaat caggtttgaa taccccagaa gcacaagaag ctttagcttt
     5761 ttatcgagat attgcacttg ttgatcatgc ttcaccaaca attacagaat tacaagatcc
     5821 tgataagtta agtcgttttc aatcaggtag attagcaatg atgattgaag gaaactggtt
     5881 tgcaccggga tttttgaaaa atgactatct agttgaaaat gttgcttctg ccgtgttacc
     5941 taagggaaaa gttaatgcaa caattacaaa tggacttggt tgggctgcca gtgcgagtac
     6001 aaaataccca gaagaagtga aaaaactaat ggcattttta gctagtgagg acgccaataa
     6061 aatccaagcg gaaacggggg catcaatccc agcactaaaa ggctttgaag acgagtgggc
     6121 tgcttcttca gatacaattg atttgagtgc ttttggaaaa atgttggagt atggagtccc
     6181 tcgtccatac aataaagatg gattacgagc tgaacaagca gaagttcaaa ttattaaccg
     6241 tattctgtct ggtgaagtaa ctgttgaaga tggaaccgct caaattgaaa aagaagtcaa
     6301 tgcgatattg agcggaaatt aaattagtta attatacaat atacaggcga aaggattcta
     6361 tttcgtctgt atattaatgg atgaatacca aaaaggggga tattatgaaa aatcgaattt
     6421 ttggaaaata tcgtaaagat caagtgatat ggggctatat ttttattttt ccaacggcat
     6481 taggattaat tttattaaat atatggccag caattcaaac agcaatttta agttttcaaa
     6541 aaacggtagg gttcggtgat actgaatggg tcggattaag aaattaccaa aaaatgtttg
     6601 cagacggtga agtttttcag tctttattaa acacattaat ttatgcagtg gtttcgatac
     6661 ctagtattgt tgtattatcc ttgttggtgg cagttttaat gaatagaaaa gttaaaggat
     6721 tgtcgattta tcggacaatt tattttctac cagtcgttgc tgcaccatcc gcagtcgcaa
     6781 tgatttggcg ctggatgttc aataatgatt atggactgat taataacatg ttggctaaaa
     6841 taggtctcga tggaccagca tggttaacgg atccatcgat tgcgatttat tctattatca
     6901 ttgtaggagt ttggagtgct attggttata acatggtgtt attactagcc ggcttataag
     6961 aaattcctaa agattattat gaagcagcgg aaattgatgg tgctagtcca atccagcagt
     7021 ttttcaacat tacgttacca ttagttacac caacattatt ttttgtagtt gtaacaacag
     7081 ttatcaatgc tttccaagta tttgatgtta tttttatgat gattactcct aatagtaccg
     7141 ccatgtttaa atcacaatca ttagcctatt tattctataa acattcattt attttaaatg
     7201 acaagggata tggttcggca atcgttatgt tccttttagt gattatatta attattactg
     7261 caattcaaat gaagttgcag aaaaagtggg taaactatta aggaggctat caaattatga
     7321 aaaataaatt tttttcaaat gcgccaatcc atatcatgct tattttaggt ggtattgcaa
     7381 tggcattacc ttttatttgg atgattttaa cttcagggaa aacgttggtt gaatctaccc
     7441 aaattccacc tacaattttc cctgagaaat ttcagctaag taactatcaa caagtacttg
     7501 atttattacc attcatggct ttttatatca atacattttt aatggttgtt ggacgagtga
     7561 ttggctcggt tttattttca gcaatggctg catatgcttg tgcgcgtcta agttttccag
     7621 ggaaaaatat ccttttcagc ttagtactta ttcaaatgat gattcccggt tccattttta
     7681 ttactccaca gtatttatta gcacaaaaat tgggtatttt aaatacaatt ccagcattaa
     7741 tattaccagg ggtagttagt gcgtttggta cgtttttatt aagacaattt ttcttaggtt
     7801 tacctgatga attggaggaa gcggcctatt tagaaggtgc caatatttgg acgatctttt
     7861 tcaaaattat gttgccgctt gctaaatcag gattggttgc acttggaata ttcacagcgt
     7921 tgtttgcatt taaagattta atgtggccat tgattgtcaa tacttcaatt gataagatgc
     7981 ctttagctcc gggattagca aacttacaag gacaatatgc cacgaacttc ccacaattaa
     8041 tggcaggttc gttattagca atttggccaa tagttgtgat attcatcgtt ttccaaagaa
     8101 aattcattga aggaattgct acaacaggta gtaaattata aaactattaa ttagtgattg
     8161 gagagtaagt acatgacaaa aattacattt attggtgcag gaagtactat ttttgcaaaa
     8221 aacgtattag gcgatgcgat gttaactcca agtctacaag atgcacatat tgcattgtac
     8281 gatattgatg aaaaacgact aaaagaatca aatttaatgc ttaaaaatat taataaaaat
     8341 tcgaatgaaa atcgtgctga tattgtttct tattatgatc gcaaagaagc gttaagagag
     8401 gctgattttg ttatcaatgc tattcaagtg ggtggttata aaccttcaac agtgattgat
     8461 tttgatattc ctaaaaaata tgggttgcga caaactattg gtgacaccat ggggattggt
     8521 gggattttta gaaccctacg gacactacca gtaatgttag actttgctaa agaaatggaa
     8581 gaagtttgtc caaatgcttg gttattgaac tatacaaatc cgatggcgat gttaaccatg
     8641 ggtgttttaa aagcgacgag tattaagact gttggtttat gtcatagcgt tcaagtatgc
     8701 gtaccagaat tgtttgagca tttaggaata gccgatcaat atgacttaaa tgactttcaa
     8761 tggaaaattg caggcattaa tcatatggca ttcttgttgg aaattacaaa aaatggggaa
     8821 gatttctatc caacaattcg tcagttagct gcagaaaaac caaatccaca tccagattct
     8881 gtacgttttg aattgttgaa acgttttggt tactatatta cagaatcaag tgaacacaat
     8941 gcagaatacc atccatactt tattaaatca aaatacccag aattgattga cgaattaggg
     9001 attccgttgg atgaatactt acgtcgttgt gaacaacaaa ttaatgattg ggaaagcatg
     9061 cgagaggaca ttgtaaataa tggggagtta acacatacga gaagccgtga atatgcttca
     9121 tatatcatgg attctattac gacaggaact ccgtcagtga ttgcaggaaa tgtcttaaat
     9181 aaaggcttaa tcacaaactt accgacagat gtttgtgtgg aagtaccttg tttagtcgat
     9241 aaaaatggcg tgcaaccaac ctatgtcggt gaattgccaa cacaattagc tgctttaaat
     9301 cggacgaata tcaatgttca agaactgaca gtagaagccg ctttaacact agataaatca
     9361 aaaatttatc aagcagcgta tttagatcca catctatcat ctgaattgtc tttaacagat
     9421 atcaaaaatt tagtggatga tttaatcgaa gcacatgggg actatttacc tgaatataat
     9481 taaaaagaag ctgaaagagt aaggaatttt caattcctta ctcttttctt agttaaattt
     9541 agtaaaaata ttgacaaaac acttagtaaa cagttacatt agtgttaaca aaaagaaaac
     9601 gcattcaaat tgtataagga ggagcataaa atgaagtggt ctaaaaaaat tattttagct
     9661 tgtatagcac taggagcaac gatctctttg actgcttgtg gaaaaaatca ggaagatgca
     9721 agcggtaagg tagaaataga gttttttaat caaaaaaaag aaatgactca aacgattcag
     9781 gagattgcta aagattttga agcaaaaaac cctgatattc atgtaaaagt tgtggatgta
     9841 ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc agatgtcatt
     9901 aatctttatc ctcaaagtat agaattgaaa gaatgggcga aagctggtta tttagaggac
     9961 ttaaccgaag aaccgtattt agagaatatc aaaaatggat atgcacaacg ttttgctatt
    10021 gaggatcgtg tatatagcat accattaacc gctaatgtct atggttttta ctataacaaa
    10081 actgcttttg aggaaatggg gattcaagca ccagaaactt gggcagaatt tgaaaaaata
    10141 gtagctgaga ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc agaaggctgg
    10201 actttaaatg gttatcacca actagctata gcgactgctg ctggtggtga agaggaagcg
    10261 aacaatgttt ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa agaaatgcaa
    10321 aaagatttta atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa caactggcaa
    10381 ggtgccggtt ataacgatac agtagttact tttacaaaag gtgatgcgtt gattatgccg
    10441 aatggttcat gggcaatgcc gatgattcat tcacaaaatc cagattttga agtaggaact
    10501 tttccttttc cagcagatga agcaggtcaa agtctaacga ttggcgcagg agacctagcc
    10561 ttatcagttt ctgcaacttc tcaacataaa gaagaagcca aacgttttgt agaatatatg
    10621 acaacacctg aagcgatgca gaaatattat gatgtagatg gtgcaccgtg tgctgttgag
    10681 ggcgttatcg agaatactca ggattcacca ttaagtggtt taactgaact tgcttttact
    10741 gatcgtcatt tagtttggtt agccaaagat tggaatagtg aaaatgattt ttatacctta
    10801 acaacaaatt atttacacaa tggcaatcaa aaagccatga ttaacgcatt aaatgctttc
    10861 tttaatccaa tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa agaaatttta
    10921 aatcgttact gggcaatcat ttttgtagca attccaatta ttttgcagat gatttttttc
    10981 tactttccaa tggttcaagg tgctttttac agtttaacta attggactgg tctgacttat
    11041 aattttgatt ttgtgggttt gaacaattat aagattcttt tttctgatcc gaagtttatc
    11101 aaatcgattg gcttcacttt aattgtgacg ctttgcatga ttgctggaca aattggtttt
    11161 ggtattttga ttgcgcgggc gttgaacaca aatattaaag ggaaaacatt ctttagagca
    11221 tggttctttt ttccagcggt cttatcgggc gtggttgttt ctttagtatt taaacaaatt
    11281 tttaactatg gtttaccaaa aattggtagt atgttaggca ttgaattttt acaaaccagt
    11341 cttttaggaa caacatgggg agcgattttt gcagtaattt ttgttttact ttggcaagga
    11401 attgccatgc cagtcatcat tttcttagcg ggttttcaaa gtattccaca agaaattatt
    11461 gaagctgcta gaatggatgg ggcgaatgat aagcaaattt tttggaatat tgaaatcacc
    11521 tacttattac caagtatttc aatggtattt attatggcat taaaaggtgg cttaacagca
    11581 tttgatcaaa tttttgcttt aactggtggt ggaccaagcg atgccacaac atctttgggg
    11641 ttacttgttt ataattatgc ttttaaaaac aatcagtttg gttatgcgaa tgctattgct
    11701 ttagtcttat ttatcttaat tgggattgtt tcactggcac aattaaaatt atcgaaaaaa
    11761 tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt tctaaatact
    11821 tgcttttatt attagggggc ttcatgattt tagttccctt agtttcaacg ctattcggtt
    11881 catttaaaac aacacccgat attatgaata atttctttcg ttttcctaat ccagcgactt
    11941 tggaaaattt ccgaagattg ttagcggatg gaattggtca ttacttttgg aattcggcaa
    12001 tcatcacaat cattgcagta gttgctgtga cagcaattat tcctatggct gcttattcac
    12061 tagcaagaaa aatgactcgt gggaaagcat acggattgat gtatttattt ttaattttag
    12121 gaatttttgt tcctttccaa gtaattatga ttccgattac cgttatgatg agtaaaattg
    12181 gtttagcaaa tatgtgggga ttaatcttac tctatctcac ttatgccgta ccacagacac
    12241 tatttttata tgttggtttt attaaactaa atgtgcctat cagtctggat gaggctgctg
    12301 aaattgatgg ggctagtcac tttacaactt atttcaaaat tgttttccca atgttaaaac
    12361 caatgcatgc gacgactatt attcttaatg ctctatggtt ttggaatgat ttcatgctac
    12421 cattattaat tttaaatcgt gattctaata tgtggacact accattgttt caatataatt
    12481 atacgggaca atattttaac gactatggtc caagttttgc ttcctataca gtaggaattg
    12541 tcacgattac actagtttat cttgctttcc aaagaagtat tatagctgga atgagcaatg
    12601 gagcagtgaa ataaccaata aattattctc attataaagg agttcataaa cttatgatta
    12661 tatttgacaa tcacacacaa acatttcatt tacaaaataa aaaaattagc tatctattag
    12721 gaatcgaaga aaataattat ttaacgcatc tttattttgg aaaaaagatt acgcactatt
    12781 ctggtgggta tcgctacccc cgaacagatc gttccttctc acccaacaat gcaaatgcgc
    12841 aagatcgttt attatcttta gacacattac cattagaatt tccaggatat ggacatggtg
    12901 attttagaga accggcgcat aatattaaat taggaaacgg tagtcgtgta aatgattttc
    12961 gttatgatag ctacgaaatt caaaaaggaa agcccagctt agcgggattg ccagccttat
    13021 atacggaaga tgatgatgaa gctgaaacgc tgattattca attgaaagac gcgattagtg
    13081 gattgacttt acagttgttc tacacaattt atcaaaatca cagtgtaatt attcgttcta
    13141 cgcaattagt aaataatagt gatgaaacag tggagattaa ccgcttagct agtcaatcaa
    13201 ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg gcaagagaac
    13261 gtcaaatgac tagagaaaaa attcatacag ggataaaagt attagatagt aaacgcggat
    13321 caagcagcca tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca gatgaatttc
    13381 aaggagaggc atatggattt aatttagttt atagcggtaa tcacgaaacg gtgattcaaa
    13441 aagatccatt tgaccaaaca cgggtcatta tgggaattaa ttcatttaat tttggttggc
    13501 aactaaaaac tggcgactca tttcaaacgc cagaagtcgt gatggtttat agtaatgaag
    13561 gtctgaatac gttatcacag acgtatcatg aattgtataa tcaccgttta attcgtggaa
    13621 actatcgttt aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg tattttgatt
    13681 ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc attgaaatgt
    13741 ttgttttaga tgatggttgg tttggcggtc gtgaaaatga cttcacttca ttaggtgatt
    13801 gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag caagtgcatg
    13861 ataacgggat gaaatttggg atttggtttg aaccggaaat gatttctgaa gatagtgaac
    13921 tatttcgagc acatccagat tgggcattgg gtattccagg tcgtgaccgc tcaattagtc
    13981 gaaatcaata tgttcttgat ttttcaagac aagatgttcg tgataatatc tatcaacaaa
    14041 tgacggcgat ttttgatcgt gtaccgattg attatgtgaa gtgggatatg aatcgaaata
    14101 tgacagaagt ctattctgtc ttgttggact caacgatgca aggtgaagtg gctcatcgtt
    14161 atatgttagg gttatatgaa tttttagaaa agttaacaac gaattatcca actattttat
    14221 ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat tacatgccac
    14281 aaacttggac gagcgacaat accgatgccg tggctcgtct gaaaattcaa tatggaacca
    14341 gcttagtgta tccaatttca agtatgggtg cgcatgtttc agcagtccca aatcatcaaa
    14401 caggaagaga aacgagttta actattcgtg gcaatgctgc gatggctggt gtttttggtt
    14461 atgagctaga tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa gaacaagttg
    14521 ccttttataa aaagcatcgc caacttttgc aatatggaac tttttatcgc ttggaaagtc
    14581 cgtttgaagg caatgatact gcatggatgt ttgtctcaaa agaccaaaca gaagccatgg
    14641 tcttttattt ccgagtatta gcagaagctg cgcatccatt agtcacattg aaattagcag
    14701 gattagcaga agaagcaact tatcaaatgg aagatgtaac atttgctggc gatgaattga
    14761 tgaatctcgg tttttatatt gactcagaac tacatggtga ttatgcgaca caacgctttt
    14821 atttgaaaca gatatagtag cttgaattta caatataagt gcaacaccaa aagagactca
    14881 ttccaaacga agaaaatatg ttttatttac acgcatttac taaaaagcag cctgatttga
    14941 attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat gattaactat tggttagata
    15001 aaggactagg cggatttaga atcgatgcga tattaaacat caaaaaaaaa ctagagtatg
    15061 gcatctttga atctgatgga gaagatgggt tagccttcat tgggccctgg attcttaatc
    15121 agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa ccacataata
    15181 gtatgacggt agcagaagcc gatgttccta atgaacgttt agatgaatat attggtgaag
    15241 atggctatta ttcgatggtt tttgatttta gttatacgga tattgatgta cctgaaactg
    15301 gtgaatggtt taaagatagc caatggacat ggacggatat gcgtaccaat atttttacga
    15361 atcaattagt tacgcaagat aaaggttggg gcgccttata tttagaaaac cacgatcaac
    15421 cacgttcaat taataaatat attccagaag aatggatcaa tgattatagt aaaaaaatgt
    15481 tggcgacgct ttttatgtta ctaagaggta ccccttttat ttatcaagga caagaactag
    15541 gaatgaccaa catagaaatg gcatcattag aagattttga tgatgtagct acgcattctc
    15601 agtataaacg ggcattagaa tatggattgg caccagagca agcattgaaa gctgtcaata
    15661 aacgaagtcg ggataattct cgcacaccta tgcaatggaa tacgcaaaaa aatgccagat
    15721 tctcaacttc tgataatgta tggttaaaag taaatcctaa ttatccagaa ctcaatgcag
    15781 aagctcagtt aaccaatcaa cagtcggtat tcaattatta tcatcaatta attaaattaa
    15841 gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca atttctacca gtgaaagata
    15901 aaaatgaaca tattttgctt tatgaacgcc aattagaaga taaaatcgta ttagttatct
    15961 taaacgttac agcaacagaa caagaatatt atgtagatga acagtaccaa caagtattaa
    16021 tcaataatta tgaagatgtt ttattagatc aaagtcgaaa attgcgttta cgaccgtttg
    16081 aaagtattgt attagctaat tatggaggac aattatgaaa attaaaaacg aagcaatgct
    16141 catcacctat gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag tcttagacaa
    16201 acatttacaa ggtgttattg gtggtgtaca tctgttgcct ttttttccat cgacaggtga
    16261 tcgaggcttt gcgccgagtg attatacgac agttgatcca gctcttggaa catgggaaga
    16321 tgtggaagca ttaggtgaag attattattt gatgtttgat tttatgatta atcacatttc
    16381 tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat gagcagtcac catacaaaga
    16441 aatgtttatc cgtattcatg aatttttccc agaaaatcga ccaacacaag aagatattga
    16501 tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg cggatggtac
    16561 gacagaagag gtttggaata cctttgggga agaacaaatt gatttagatg tgaccaaaga
    16621 agttgtgaaa gaatttattc gagaaaccat caaagatatg gcgaaccatg gctgttcttt
    16681 aattcgatta gatgcgtttg cttatgcgat taaaaaatta gataccaatg atttcttcgt
    16741 ggaaccagat atttgggaac ttttagacga agtgcgtcaa gaagcagcga aatatgacgt
    16801 cgaattatta ccagaaatcc atgagcatta ttccattcaa atgaaaattg ccaatcatga
    16861 ttattatatc tatgacttcg ccttgccaat ggtcactctg tattcacttt atagtggaaa
    16921 atcagaacgt ttagccaatt ggttgaaaat gagtccgatg aagcagttta caacattgga
    16981 tacgcatgat ggaattggtg ttgtggacgc tcgtgattta ttaacagatg aagaattgga
    17041 ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct attcaagtgc
    17101 caattacaat aatttagata tttatcaaat taacagcacc tattatagtg cattaggcga
    17161 taatgaccgt agctatctgt tagcacgtgc gattcaagta tttgcgccag ggattccaca
    17221 aatttattac gttgggttat tagcaggaaa aaatgatatt gaactgttgg aagcaacgaa
    17281 agaagggcgt aatattaatc gtcattacta tgatttagcc gaaattgaag aagaagtgca
    17341 acggccagtg attcaacaat tatttgcact tttaaaattt agaaatgaat ctgcggcgtt
    17401 tgatttggat ggaacgattg aagtagcaac gccatcagaa tcagaaatag tgattacacg
    17461 taaaaataaa aacggtagtg atagtgcaac attaacggct aacttggata cgaaagaatt
    17521 tatcattgag tctaaagata taaaaatttt cactagtgtt gcattaaaat tttaacctat
    17581 tccatagaac aaaaaatcct ttatactagc acttggatga cttatccaag accaataaaa
    17641 aggactcata catggataag tataaaacaa atacagcatt taataaatgg ttttctgcca
    17701 taaaattagg aaaactttct gtcaactccc aaaaatgtat ttttgattac aattcgtatc
    17761 gtaaaaaact gtcttttgaa gctgtgctca agctctttct ctacgcagtt aatgaagaaa
    17821 aagaaagttt gcgtgatttg agtacagctc ttatcgaccg atcgctccaa ctagaaaccg
    17881 atgtgtcatc gattagtcat acccaattgt ctcgagcatt taatgcactt gaacctaaag
    17941 tgttagaaga aatttttcaa caactgttag aaaaagttca acatcaaatc aaaccaacca
    18001 agcgaaatca tctgtatctg attgattcca gtacgttttc gcttagttta aaacgtcata
    18061 agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac tattgttata
    18121 tggacgatac tacgatgtat ccaactgatt ttaccttaac aaatgcccat gaacatgatc
    18181 tcaatcaact accagtattg gtgaatcaac cagaggcaac ttacgtgttt gatcgtgggt
    18241 attaggactt tgaaaagatg gatcagatgc attgggatgg ctatttcttc gtcactcgga
    18301 ttaaaaaaaa caccaaagtt cacgtgatag atactctgga aacatcgcca gaaacagaaa
    18361 ttctacgaga cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc aatttccgac
    18421 tagtcactgt tcaagataag aacggacgtg tgtttcaatt tatcaccaat cgaatggacg
    18481 tctcttcaaa agaaatctct gatatgtatc acgctcgttg gcaaatcgaa ttgtttttca
    18541 agcacatcaa acaacacatg accattaaaa ccttcttttc tcaaagtgaa aaaggcgtgc
    18601 agaatcaatt gattttaacg atgatttccg ctctcctcac gtttctcatc aaattggaga
    18661 cgaaaacgga aaaatcagtg ttccaaatca aacgattctt tcgttattta ctgtttcagc
    18721 cctttgaatg ttgtctagaa aaattaattc ccacatgaga aaaaagaaat ttggctcgtt
    18781 acttttggtt ttcagttgaa taacagataa aaaattttct ggaaaaagtc atcctctgcg
    18841 tcagcacagt tttttttgac ttattttcag aggaaatagt tttcagaata ttctaaaatg
    18901 ttttatgcaa cactagtgat attttttaga attagatttc taatgtgcat tttttgagta
    18961 ttaaggagca agtatacaaa gatttataaa gctgattaaa tttgaataaa aatatgcagg
    19021 aacgctacta tttttagtag tgttcttttt tatatttgaa taaaaaatgt ggtggttaaa
    19081 tggtggttat aacaaaaaat gaattaatga aattaggtta tgggaagtat cagtctgaga
    19141 gaatcatacg acaggcaaaa gcaattatgg tttcaaaaag ctatacctat tacgaaaata
    19201 gaagattagg actagtacct gttgaatctg tagaagaagt tttaggtatg aagatagagg
    19261 tagaggagga ttttcagaat catggttaat tttaaaaaag ataaaaatgg gaaatatcat
    19321 tttactgcta gtttgggatt cgacaaacga acagggaaaa gagtacaaaa aaacgtagag
    19381 gggttaaaac agttaaagaa gaaaaaaagc gtataccgaa cttgtttcta attatggaaa
    19441 agaagcagaa acttctcaaa gtactatgtt gtttgaggag tttttttata aattattcct
    19501 accttattat caagggaaag tagaagaaag aacatataaa aatcggttat ctacaatcaa
    19561 aaaaactttt cttatttctt taaaatggag ttaagagaaa tacgtccaat tgacattcaa
    19621 aagtggcaaa ataatttatt gagcaaatat aaaaattctt atgtaagaaa catgtatggg
    19681 atttttcaga tgtcattaga tcgagcagtt gttttgggac tacttgaaaa aaatcctgca
    19741 aaagttattg gaaatgtgaa gaaaaggaaa actacagttg atttttggac aaaagaagaa
    19801 tttacaaaag ttatttcaac tttttatatt gatgatttgt accaacattt tggttttatt
    19861 tgtatgtggt tactttttat gaccgttatg cgtttgggag aagccactgc attaacttgg
    19921 aaagatattg attttgaaaa taaaaacttg cgtgtgaata aaacactcta ttataagaga
    19981 gccaaaacct atgagtttgt agaacccgaa acggcttcta gtattcgtac aatcgaacta
    20041 gacgacaata caattacctt tttaaaagct tggaaggagc gtcaagtttc aataggaggc
    20101 atgtcgtttg tactttctta taatggaaca cctactcaga aatatacaat ccgtcatatt
    20161 atgagaagac atgcaacgat cgttggtgtt catataatcc gcatacacgg attaaggcat
    20221 agtcatgctt ctttgcttat atcacaataa aaagatgcac ttcgaattaa agaaaggtta
    20281 aaacatgctg atgtacaaac gactttagga acttatggtc atttgtatct aaattcattg
    20341 agagagataa caaatgatcc cacagattat atccaaacta atttttctaa aatcaaaccg
    20401 acacctattt acgataataa aaaataaatg ataaacaaaa gtaggatcac accaaaagaa
    20461 acacactaga aaaactataa tacaggcttt tctagcgtgt tgtttcttat ttccgcctat
    20521 ttatacatta tactcataaa gcatttaatg catcgatcgt agccgcattg tctatatggg
    20581 tatataaagt tgttgtattg gtacttgaat gtcccaattg ttgggcagtt aataccaatg
    20641 aatttgtctg ttggtagagt ctagtagcta atgtatgacg cagtttgtga gggctgactt
    20701 gaattctaaa gccttttgaa tattttgcta ccatccgttc gattgcaata ccggatatac
    20761 gcttggcttc tccttggtaa attgctagaa ataaagcttt ttcgttttct gttactttat
    20821 atcgttcttt ccgaatagct agataattag ctaaatattc cattgcgaat gctgctatat
    20881 tgacagcatc tcttttattt ccttttctag ttactaccac attattctta tctaaagata
    20941 ggtcatctaa attaatattt ataacctcgg agagtctcaa tccagatcct aaaatcagtg
    21001 cattgatcgc caggtctctt tctacatctc gttgataata gatcaaagct cgtttactca
    21061 aagtattttt atattcattg gcaatataat ctagataatt ttgtgtatcc ttatctagaa
    21121 ataatttttt ctcaatggca ttggcacgtg cagctaacgt agttttatct gtgatggttg
    21181 ctacttgatc cataggatta ttgtctaagt atggttttcc tagaggatta ttggaacttt
    21241 tcgaaagata tttaaataga acctttaatg ccgtgatatt tctattgata gtagaagtag
    21301 atagacctaa gttattatta tctttgagct tagtttttgt tgtctctttt tttacatttt
    21361 cttttgtgcg acgtttcaaa tgagatttgt acaagtttag gtcataggca gttaactgtc
    21421 ttatcgaggt gatagaagta tcaggaacga tagactcact cagaagccat tcataaaaga
    21481 gcttataatt ttttagatat tctaaagtgg ttcgaggtga ttgatcttta tcctcacgat
    21541 cttgaacaaa atcttttata taatcgggca tatgaagtat gattgtatct attttttgat
    21601 atatttctgt atttttcatg tcattcatcc tttcaataag taggggagaa gagtatctct
    21661 ataattggac tataccaatt atagaatgtt ttgtcaattg gtatgctcta aaatttatta
    21721 atttgtttca aaaaaagcat ataaacaggt atacgtttaa taatttaaaa taaatggtat
    21781 acataaatag ggaaggaaat agaagggagc aatctaaatt ttttgaattt tcttaattaa
    21841 ttatatagaa agaacaatct ttctatagag ttagacatga gagggactat gcttgattta
    21901 aaatcaacaa gcgtatatac tgtatataca aagaaaaagg agggagaaga ctatggcaat
    21961 taaagaaaga aaatttaggg aagtggggaa ctcagtagta ttaactattt caaaggagtt
    22021 tcttaaagaa aatgggttaa atcccggaga tacggttttt ttagatgaag aaaaacttcg
    22081 agcggcaatt acaaaaaaac aagccttatc tgatgaaatt gatttagcaa tatctcaagc
    22141 attaactgaa tatgatacta cttttagaga gcttgtcgat aaatgagtat tcattaccta
    22201 gaagccaaag atattataaa aatgaatgta atccaaataa aaaaatactc tcctaatgaa
    22261 ccccttggtg taaaggatgc gaatgcttta gaaatgtgtg tagggcaact tcgagcctca
    22321 gcttttggtg aagaggttta tccttcgatt tatgaaaaag cagctatcct acttatacaa
    22381 ttgataaaga aacatccttt tcacaatgca aataaacgta ctgctttctt agctactttt
    22441 gtctttttga aaatcaatgg acagctttta gctattgatc aaaaagatgc tgtaaattta
    22501 gcggtatata ttgctacgta tgataaggat ttcgatcaac taaaatatga agtgatcaac
    22561 actataaaga cacatacgca gcccatttaa ggagttactg tatgtgtctt tatagggtta
    22621 ttagctgcta tagcgaaata attgaagtaa attacaaata gctataattc aaatccaact
    22681 atatagaaaa cgtaaatttt ctatatagtt ggattaagat aacgactatt tattttatca
    22741 tatttttact ataccctata cttttacttg gtatatttat gaataatcgc aaatttaccc
    22801 ctatactact agtttgtaag ttagattaat aaaaatacta aagttatttg ttcttttatt
    22861 aaatacacct acaacttata aattgatggt atttagataa cacgaaagaa taaaaataac
    22921 agctgatagt aaagtttgtt gacgattgct actcaatcga aagtgagtta gtgcgtatga
    22981 acttgcttct gaaaacttat gaaatgaaag accttttaca tcttgcagaa atcggtactg
    23041 ggattaaact aagcaataaa aactaatgtt cccctcattc tttgaacttc tactatttga
    23101 tttatttatt ctgataaatg acgatttcac cttcaggatc ttcaaatccc gtatagaatt
    23161 tatttaagat ctcttcagta tcagtaatag gataggcgat aggccacata ccctttgatg
    23221 gttagaataa actgctcaaa atctgtatcg tatgttggct ttagcatatt aacaaaggct
    23281 atctccaaat tataaaccat aagcgttgaa tcgttcttaa caatttcaat tcttcctaac
    23341 ccaaagtcag aagatataat taccggactt gccttaccct tcatgtgggt tgttgagatt
    23401 ccatatttaa agggaattgt cggtactatc ggcggtctga aaaacatccc aaaaataaaa
    23461 aaatctttcc cctgctagtc tcctaagtcc ctttgtagcg gatatggcac cataatctca
    23521 ttttatcgaa gtgagtcatg gtttatctgt tggccaacca aaattgatac aatctaacga
    23581 tgcaccctct acggcattct aggggagtgc acttgcattc acccaataaa aaccggcagt
    23641 tagcaaagaa cttatgtagt cgtatgttct tactgttaac atacaagagg tctcctttcc
    23701 acaattgctt agcagtaaac gggaatttac aaagctaatc tcttccattt tcttatcttt
    23761 gttctaaaag tcccaaatgg agcaactgta tttacatgaa taaatttata tacttcccat
    23821 acagccgttt tggttgcctc gtctgcccat tttcttttgt gtgcagtgaa tagatcttca
    23881 tcactcattg aatcaatcat catgttaata tcatcaatat tacgattgag aatttcctct
    23941 aattctttca atggtagatg tgcataggta tttgtaaacc acttatataa ctctcctaac
    24001 tgattccatt taaattgatc agaaggcgtt ttaacagata atccatttct ttcatcattt
    24061 tcccatttta gaattaaatt cgtccaacca acttgatatg ctaaattttc agcaggtgtt
    24121 ctatccactt cctcaactct tttatccttc aattcttcag gaatagtttc aaattctaaa
    24181 atatatttag tataagtcct atttatttct ttttttaatt cttctttatt tttatatgtt
    24241 ctcattatat tacctccata aattcctaat tgtctccttt aattcatcta taatttcatc
    24301 cacgatattt taatccgttc tacaatgcat atctgatatg cattgactgt ctaactcata
    24361 tgtgaatgat ttataaaata tcccaacgtc tcatctaaag aaatgttgat cgtcagcaag
    24421 gagctaaaaa atacgaatat atttcttttg tagtaaaatc ggactggttt gcatatcatt
    24481 ttcttcgtaa atatcaaaat catgttaact actatcaatt actaataaaa actaccactt
    24541 taagcgttaa gaactgttaa gtttagttaa gtgaggatag ttcacaaaat ccattgttaa
    24601 acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg attacagcat cgctctttct
    24661 tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat tgacgatgac acgttcctta
    24721 cggctcatca gatgaaacag ctacaaaaac gaaatgatca actcgaagaa aatttaattc
    24781 taaaaaagcg attgccatat tcacgcctcg ctcgacgaac tattaaaggt caagatttac
    24841 ctgaagatca tcctttttag gaaattgaag ctttttatgt tgcacaatgc atttataata
    24901 ctacaatgat gttttcacca gatattcttc tagggtgcgg tgtaatgaaa cagatacact
    24961 tgcaaaagaa aattcaccgc gaatttacta aaattatgaa taattatgtt aatcttccga
    25021 aatttttttc caaaattaag ggatcaagct ggtgttatag attgtttaag gttggcaaaa
    25081 aaataacttc tttttaaaca attatagaaa accgatactc tgttaagttg gttgacacgg
    25141 tgtcgatttt tatgactttc ttattctgaa acttcactaa cggacagtac ctaaaaagtc
    25201 tatagtatgg acgtttacga gtactatttt atcaaaaatg ctgctcattt caacttatat
    25261 gttttagtga actaccccca cttagc
//
LOCUS       DANJMA010000036        25242 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000036 DANJMA010000000
VERSION     DANJMA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..1087
                     /locus_tag="PWU31_001784"
     CDS             <1..1087
                     /locus_tag="PWU31_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1084302.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="PWU31_001785"
     CDS             complement(1305..2447)
                     /locus_tag="PWU31_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1084303.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="PWU31_001786"
     CDS             complement(2452..2637)
                     /locus_tag="PWU31_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1084304.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="PWU31_001787"
     CDS             complement(2991..3236)
                     /locus_tag="PWU31_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084305.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="PWU31_001788"
     CDS             complement(3230..3631)
                     /locus_tag="PWU31_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1084306.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PWU31_001789"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PWU31_001789"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL1084307.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="PWU31_001790"
     CDS             complement(5649..7415)
                     /locus_tag="PWU31_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL1084308.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="PWU31_001791"
     CDS             complement(7428..8222)
                     /locus_tag="PWU31_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1084309.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="PWU31_001792"
     CDS             complement(8232..9608)
                     /locus_tag="PWU31_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1084310.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="PWU31_001793"
     CDS             complement(9728..10375)
                     /locus_tag="PWU31_001793"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL1084311.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="PWU31_001794"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="PWU31_001794"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1084312.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="PWU31_001795"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="PWU31_001795"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1084313.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="PWU31_001796"
     CDS             complement(13042..14673)
                     /locus_tag="PWU31_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL1084314.1"
                     /translation="MVSIKNWRNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="PWU31_001797"
     CDS             14977..15648
                     /locus_tag="PWU31_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1084315.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="PWU31_001798"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="PWU31_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="PWU31_001799"
     CDS             complement(16805..17257)
                     /locus_tag="PWU31_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084316.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="PWU31_001800"
     CDS             complement(17264..17812)
                     /locus_tag="PWU31_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL1084317.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="PWU31_001801"
     CDS             complement(17857..18189)
                     /locus_tag="PWU31_001801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084318.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18460)
                     /locus_tag="PWU31_001802"
     CDS             complement(18203..18460)
                     /locus_tag="PWU31_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084319.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="PWU31_001803"
     CDS             complement(18655..19197)
                     /locus_tag="PWU31_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084320.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="PWU31_001804"
     CDS             complement(19218..20309)
                     /locus_tag="PWU31_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL1084321.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="PWU31_001805"
     CDS             complement(20317..22527)
                     /locus_tag="PWU31_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084322.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="PWU31_001806"
     CDS             complement(22543..25071)
                     /locus_tag="PWU31_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1084323.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
BASE COUNT         8057 a         5080 c         3438 g         8667 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt cgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aa
//
LOCUS       DANJMA010000037        24585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000037 DANJMA010000000
VERSION     DANJMA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(159..557)
                     /locus_tag="PWU31_001807"
     CDS             complement(159..557)
                     /locus_tag="PWU31_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL1084324.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(554..1327)
                     /locus_tag="PWU31_001808"
     CDS             complement(554..1327)
                     /locus_tag="PWU31_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL1084325.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1324..2007)
                     /locus_tag="PWU31_001809"
     CDS             complement(1324..2007)
                     /locus_tag="PWU31_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL1084326.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2174..3061)
                     /locus_tag="PWU31_001810"
     CDS             complement(2174..3061)
                     /locus_tag="PWU31_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084327.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3058..3927)
                     /locus_tag="PWU31_001811"
     CDS             complement(3058..3927)
                     /locus_tag="PWU31_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084328.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4064..5350)
                     /locus_tag="PWU31_001812"
     CDS             complement(4064..5350)
                     /locus_tag="PWU31_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1084329.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5623..6201
                     /locus_tag="PWU31_001813"
     CDS             5623..6201
                     /locus_tag="PWU31_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1084330.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6201..7130
                     /locus_tag="PWU31_001814"
     CDS             6201..7130
                     /locus_tag="PWU31_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084331.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7127..8863
                     /locus_tag="PWU31_001815"
     CDS             7127..8863
                     /locus_tag="PWU31_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1084332.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8926..9762)
                     /gene="pheA"
                     /locus_tag="PWU31_001816"
     CDS             complement(8926..9762)
                     /gene="pheA"
                     /locus_tag="PWU31_001816"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL1084333.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9759..10268)
                     /locus_tag="PWU31_001817"
     CDS             complement(9759..10268)
                     /locus_tag="PWU31_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL1084334.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10261..11556)
                     /gene="aroA"
                     /locus_tag="PWU31_001818"
     CDS             complement(10261..11556)
                     /gene="aroA"
                     /locus_tag="PWU31_001818"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL1084335.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11569..12660)
                     /locus_tag="PWU31_001819"
     CDS             complement(11569..12660)
                     /locus_tag="PWU31_001819"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL1084336.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12677..13843)
                     /gene="aroC"
                     /locus_tag="PWU31_001820"
     CDS             complement(12677..13843)
                     /gene="aroC"
                     /locus_tag="PWU31_001820"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL1084337.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13845..14909)
                     /gene="aroB"
                     /locus_tag="PWU31_001821"
     CDS             complement(13845..14909)
                     /gene="aroB"
                     /locus_tag="PWU31_001821"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL1084338.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14942..15964)
                     /gene="aroF"
                     /locus_tag="PWU31_001822"
     CDS             complement(14942..15964)
                     /gene="aroF"
                     /locus_tag="PWU31_001822"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL1084339.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16623..17597)
                     /locus_tag="PWU31_001823"
     CDS             complement(16623..17597)
                     /locus_tag="PWU31_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL1084340.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17578..19461)
                     /locus_tag="PWU31_001824"
     CDS             complement(17578..19461)
                     /locus_tag="PWU31_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL1084341.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19479..21704)
                     /locus_tag="PWU31_001825"
     CDS             complement(19479..21704)
                     /locus_tag="PWU31_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1084342.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21819..22853
                     /locus_tag="PWU31_001826"
     CDS             21819..22853
                     /locus_tag="PWU31_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084343.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22920..23933)
                     /locus_tag="PWU31_001827"
     CDS             complement(22920..23933)
                     /locus_tag="PWU31_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084344.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24011..24199)
                     /locus_tag="PWU31_001828"
     CDS             complement(24011..24199)
                     /locus_tag="PWU31_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL1084345.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7069 a         5341 c         4397 g         7778 t
ORIGIN      
        1 taaaatagca tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta
       61 ctatttcata agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg
      121 ccttcttgtt tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta
      181 tcgttatttt catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta
      241 gtgataccaa tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg
      301 atgttgatgc cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat
      361 gtcaacacaa tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt
      421 acaccagaac ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag
      481 tcgataacat cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat
      541 gctacaagat ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt
      601 ctttttcatc ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca
      661 atgcaatcat tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg
      721 cagcgattgc cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc
      781 caccgttatc aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg
      841 ctagcccgta tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca
      901 tagacaatcc tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc
      961 ggattctccc ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag
     1021 caaaccctac taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca
     1081 atcccgcaat agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt
     1141 gcaggaaaag gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac
     1201 gctttttgcg catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg
     1261 ccactgccaa aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga
     1321 aactcattgg aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat
     1381 accgaccatt ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc
     1441 tccggcaagg gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc
     1501 aaaagcaatc ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta
     1561 gcctatagcc atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc
     1621 tagaaatact ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc
     1681 gtcagatata tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat
     1741 cacgatcccg atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat
     1801 atatttccct actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag
     1861 cgtcattccg atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc
     1921 taacggtgca aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc
     1981 aatcacttct ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt
     2041 aaaatgaaag agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata
     2101 aaataacaca cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc
     2161 ttattgtgtt tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa
     2221 caaaaaatac gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat
     2281 agtctggtgt ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag
     2341 gagattgcaa agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga
     2401 ttgcagccgc ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta
     2461 gttcttttag accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat
     2521 tttgtcgaaa gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga
     2581 aactgttgat tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca
     2641 tcaatgccgc aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc
     2701 gtttactttg ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca
     2761 cagtggtcac caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat
     2821 tggaaaacaa attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg
     2881 tgatatccac gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa
     2941 taaatgatag gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt
     3001 ctaatgcttg ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca
     3061 tcgactttct cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat
     3121 aacaaaggaa actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata
     3181 gatgtattga gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac
     3241 ttcatcaaaa agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg
     3301 ttttaaattt gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga
     3361 tgctgcttca taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt
     3421 gtatccagtc caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg
     3481 agtcaaccag ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag
     3541 gccagattga gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca
     3601 tggaagaaag atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag
     3661 gttggaaata aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa
     3721 tgtattaaaa aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa
     3781 cccgttaaat gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac
     3841 catcggaata aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc
     3901 gtttttgaat aaatctttct gcttcattct tttccctcca attctatcat tgattatcta
     3961 catgaaaaga caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt
     4021 ttgtctgatg aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg
     4081 cttctacttg tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc
     4141 gatgaagagc ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt
     4201 ctggtatttc agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt
     4261 cagaagcttc ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga
     4321 tttctttcgc aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat
     4381 ctttcgctga atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt
     4441 tagaagcttg cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt
     4501 ttccagattg gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag
     4561 aagctactgc tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga
     4621 ctagatcttc tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt
     4681 cttggatcgt gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa
     4741 tcatccttac tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt
     4801 ctttgacatc tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt
     4861 ctgtataacc tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat
     4921 cgaaaggaac accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa
     4981 aatgatcttc ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat
     5041 agccttgtat cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc
     5101 tcgctaaagc ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta
     5161 tctctgtttc ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg
     5221 tctcatccca cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc
     5281 cgcatcctgt taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc
     5341 tgatgttcat ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca
     5401 tcataccaaa atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa
     5461 aataataaaa aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa
     5521 gatcgaaatg gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa
     5581 gtatttttaa attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt
     5641 cttatcgatg atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag
     5701 cacgggtttc aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg
     5761 gtacatgcaa tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa
     5821 ttatcgcggc tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat
     5881 gatgaatttg atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag
     5941 cctcgattga gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa
     6001 aaaaagccca acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca
     6061 cagcagattg ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg
     6121 tttttctgtt atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac
     6181 agtcgatcac ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta
     6241 ccttttcgga agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt
     6301 ttgttcaaaa gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt
     6361 ttgcaatcca agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac
     6421 gtttctatca taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca
     6481 accacgagca gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag
     6541 ctgtcaaaga acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt
     6601 atttgaaaca tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc
     6661 atgttgggaa tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg
     6721 ctgagtattt cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact
     6781 tttctgcaaa tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc
     6841 aggaaaatta tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat
     6901 atagctatct ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa
     6961 aaaaagtccg tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg
     7021 aaatctgtga gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag
     7081 agacaggaat gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat
     7141 caaagagcat gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca
     7201 agccatcgtc atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt
     7261 caatcaatcg aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga
     7321 tacaatcgag aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc
     7381 caatgatcca gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa
     7441 tgactctctt tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa
     7501 tggaagaact tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca
     7561 atctgcgatt gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca
     7621 ccacggattg actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa
     7681 aaatacattg aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc
     7741 gaatcttttt caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga
     7801 acagacgatc atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa
     7861 aaaactaaca gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct
     7921 gtaccgccag gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat
     7981 gggcgtgatt tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt
     8041 ggtatttatg tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa
     8101 acggctagag gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca
     8161 attgctaggg aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac
     8221 gatccaagac gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca
     8281 gccccatttt atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac
     8341 agatgaagca gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa
     8401 aaaagaagaa gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct
     8461 tttgagtatg cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca
     8521 gtcattatct atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc
     8581 ttttcacaat aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct
     8641 tttattcgaa gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa
     8701 tgatcatttt tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg
     8761 cgatgcatat ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg
     8821 tctaccgctt cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg
     8881 atttttttat tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc
     8941 ttctactgtc agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat
     9001 ttcttggagc gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa
     9061 aaaatattct cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc
     9121 ccgccagcca aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt
     9181 caagatgaca ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac
     9241 gatccaaaaa cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa
     9301 tccaacatgc tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc
     9361 tgctacatac attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata
     9421 atgcgtttct aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt
     9481 aatctttgtt gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt
     9541 aacttcgact tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa
     9601 tgaattctct acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca
     9661 aacagggatg gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg
     9721 ataagtgaat gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct
     9781 ataatggtat cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca
     9841 ctttcttcat accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt
     9901 ccaagagcta aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc
     9961 gcttctgcct ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca
    10021 ctttggacaa tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa
    10081 atatctgact ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc
    10141 gacaattgga tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat
    10201 tgagctaacc ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg
    10261 ctagccatga gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata
    10321 agagattgaa acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc
    10381 tacttgcagc atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag
    10441 aggtgtcggc ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt
    10501 ctgtaattct tctgccaccg catcaattct gttggtttct tttactttta attcttctgc
    10561 gtctttgatg atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc
    10621 atcaatcaga cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt
    10681 gacacttaaa tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc
    10741 catctcctcc aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa
    10801 caacagttgg ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat
    10861 atcacctgga acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt
    10921 tttatcttcc actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg
    10981 ggaacgttct ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa
    11041 agcagatttg acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg
    11101 ggttcctgtc acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc
    11161 catctcgcgt aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc
    11221 gaacagagta gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt
    11281 accggagttt cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc
    11341 atgaatcttg atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg
    11401 taaagtactc agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc
    11461 aattgctcca aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt
    11521 ccctcttaat ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca
    11581 gcaatgataa ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt
    11641 tttaaaactg atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt
    11701 aatcaaagaa actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc
    11761 atctggcaca tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg
    11821 caactggtct cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc
    11881 agtcatgatc cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa
    11941 taatatctgc ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat
    12001 cctagtgata tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt
    12061 gaattgttct gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat
    12121 tcctgttata cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca
    12181 aaatagttct tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta
    12241 ataggcattt tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc
    12301 cattggatgt ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg
    12361 cttcgtactg ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac
    12421 atcaagataa gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc
    12481 tgcacctgtt tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc
    12541 acgggttttt gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat
    12601 acaaagtgcc atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat
    12661 cttatttgcc ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt
    12721 tcaactcaac aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg
    12781 ctacaacatt ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta
    12841 cacttgcttt gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg
    12901 taggaatggg tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt
    12961 cgaagccgcc tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct
    13021 catccatcac ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc
    13081 ctttaaaggc attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc
    13141 attgtacgta gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc
    13201 cgatcgtatc tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg
    13261 ctggatcaac aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga
    13321 tatttccagg tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga
    13381 tgtcaagttc ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct
    13441 ctcttgcaga ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc
    13501 ctaccaaatc cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt
    13561 caggcacttc tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca
    13621 ctaaagtgac aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac
    13681 gatctttttc gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt
    13741 cgtgattgat ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg
    13801 ttaattctgg tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag
    13861 taatctttga atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc
    13921 aagatgatat tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat
    13981 tcatctggat gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct
    14041 aaagcagctg tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct
    14101 accattccaa tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca
    14161 attgcatggc caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca
    14221 tcttcttcca ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt
    14281 tccagaagat ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct
    14341 atagctgctg atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt
    14401 tttagaacct caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt
    14461 ttcgctttgt ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt
    14521 agcgtagtcg gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct
    14581 gctagatcac ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca
    14641 ttatctgcta aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc
    14701 gccggtacga ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt
    14761 tctgcatata gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa
    14821 atcgtttgaa gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat
    14881 ttttggttat caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga
    14941 attaatcgag tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca
    15001 tgcgtgagta agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat
    15061 caggatggat ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg
    15121 gtggtacaag ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag
    15181 ttaatttttt catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt
    15241 aagtccgaat cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt
    15301 ctgcagcatt aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt
    15361 tacctgtttt accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct
    15421 gtaggatatc gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa
    15481 tcactttcat tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta
    15541 aaccttggaa agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg
    15601 cgcccccggc tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac
    15661 aaggaccagc catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga
    15721 ctgtattgcc aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag
    15781 cattttctac tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt
    15841 ttagtccaat cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt
    15901 cgatatgatc gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta
    15961 tcattgtttt tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt
    16021 ttcaaggtgg ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg
    16081 ctccttcttt cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat
    16141 tgcctctaca gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc
    16201 cactatagat gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat
    16261 agatacaaac ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag
    16321 ctaaagtaat acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa
    16381 ctgatgtaag aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg
    16441 aaactttttt taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt
    16501 attgaagaaa atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga
    16561 catatagaat aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat
    16621 tgctactgaa taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac
    16681 tcaacatccg ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta
    16741 agaaccgttc gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct
    16801 gctttgaatg gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa
    16861 aaatcttctt gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat
    16921 ttcgtttcca tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct
    16981 gccactttat aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat
    17041 aggccttcat actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg
    17101 ggtaattccg gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct
    17161 ccagcgattt ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta
    17221 aaaatgattt caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca
    17281 atcggcaaat cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac
    17341 aatccggctc ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg
    17401 gtcgttgctc gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta
    17461 ttgagcgatt ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg
    17521 acacctaatg tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta
    17581 tttactgtat ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc
    17641 tgcaaaaacg atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc
    17701 gccatagcgc atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt
    17761 aaaatcccat atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga
    17821 atccattcca cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt
    17881 ttccgcttta ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt
    17941 tacatctgaa atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag
    18001 tcgatcgggg tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata
    18061 agattcattt ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt
    18121 cgtttttgct cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta
    18181 agaaggctca aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc
    18241 catcacatgg attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg
    18301 agatgtccgt acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc
    18361 ttctatagtg atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat
    18421 aatcatccgt tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc
    18481 gaaggaatag tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag
    18541 cttatataaa ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg
    18601 aatcgtcatt tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc
    18661 tagtatcgct acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc
    18721 aagtttgcca ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg
    18781 gttttgtcct cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc
    18841 ctgatcttcc aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc
    18901 ttcttccaaa taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc
    18961 gataaaatga ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga
    19021 gatttgtttt ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg
    19081 attgtcttta gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg
    19141 gggacggcga aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc
    19201 ataatttgct aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa
    19261 atctttttta taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca
    19321 tgatccattt aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata
    19381 acaaacatga tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa
    19441 ccattttata tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc
    19501 agttctgttt ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg
    19561 gcccccgaaa atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc
    19621 atcagatata cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca
    19681 atagcttcat cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga
    19741 ctcgccaaac gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata
    19801 atttggtctt ttacttgctt tttttcttca ggagacagct ttgtcaaatc cagttcataa
    19861 ccaaaaacag cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc
    19921 tgatgatttg gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt
    19981 gttccatatt ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc
    20041 ggcatataat aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat
    20101 aagatttccg gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa
    20161 cgatgcgcta cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc
    20221 gaacggttca tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt
    20281 tgtttctcaa tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg
    20341 gaagcagaag gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt
    20401 tcagaatcaa ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta
    20461 tgagcataat ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag
    20521 tctcctaatg aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac
    20581 atttcgatgc ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag
    20641 tcaaagtaag tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga
    20701 cctcttgcga ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc
    20761 tgacttgagt aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc
    20821 cacgaaaaac gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt
    20881 tcaagttcga aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta
    20941 ttttctgtcg tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt
    21001 cctcgacggc tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt
    21061 tcattgacat gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag
    21121 tctagctgga aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc
    21181 tttacagatc gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa
    21241 atcgctgttt ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc
    21301 acataagttt gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca
    21361 tatctcagat ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga
    21421 aagtccatgc tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg
    21481 tcaggattta agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg
    21541 tctttgtact gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct
    21601 atctttatca gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca
    21661 aataagatga aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca
    21721 aaacggaaac cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt
    21781 caataaaaat tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg
    21841 cacgaaaggc aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga
    21901 tccatgtggc tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg
    21961 acatcagtac tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga
    22021 tcggcaaagc gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag
    22081 aagaagctcg acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta
    22141 aacaatggga agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag
    22201 aatcgattac cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca
    22261 tccagcaaga ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg
    22321 aattgttctt agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata
    22381 tggatgaatt cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc
    22441 attttatgat cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag
    22501 aaaaacaagg tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg
    22561 ccattcttgt cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa
    22621 atatccaaat tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa
    22681 acctctcccc tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg
    22741 ttcgaatggc attggagcga attgacggta tacgggacga accaatcatt ttgacttatc
    22801 ctgcacgatt gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag
    22861 gttgtgaaaa gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct
    22921 tacttatact tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc
    22981 gtgtaatcaa attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat
    23041 tgcacgggta cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat
    23101 gagtatgcat ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc
    23161 acactagctt ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac
    23221 gcaacggttt tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc
    23281 ttcaaatcaa caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc
    23341 tgtttgattt tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat
    23401 gcgatttgtg aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg
    23461 tcgtcttttt cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta
    23521 gggtattctg aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct
    23581 gaaactaatt ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca
    23641 ccatgtaccc aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca
    23701 taagtattct ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt
    23761 tctgcttgat cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg
    23821 atcaagaatg ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc
    23881 ccaagaatga ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc
    23941 cctcagtagt tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct
    24001 cctagatgat ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt
    24061 ttttccattg ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag
    24121 gacttaagca caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca
    24181 cttgctggtg ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat
    24241 ttatcttcag agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct
    24301 aaataatagg cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc
    24361 cctgatgccg ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa
    24421 tcatttgaga atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa
    24481 cgaaaaatct gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa
    24541 cagtccttat tggcaaatcg ccaaaataat gtagactaac taaaa
//
LOCUS       DANJMA010000038        23670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000038 DANJMA010000000
VERSION     DANJMA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWU31_001829"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWU31_001829"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL1084346.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWU31_001830"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWU31_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL1084347.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWU31_001831"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWU31_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL1084348.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWU31_001832"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWU31_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL1084349.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWU31_001833"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWU31_001833"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL1084350.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWU31_001834"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWU31_001834"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL1084351.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="PWU31_001835"
     CDS             complement(8557..9027)
                     /locus_tag="PWU31_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL1084352.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="PWU31_001836"
     CDS             complement(9073..9498)
                     /locus_tag="PWU31_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL1084353.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="PWU31_001837"
     CDS             complement(9565..9834)
                     /locus_tag="PWU31_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL1084354.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="PWU31_001838"
     CDS             complement(9845..11440)
                     /locus_tag="PWU31_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1084355.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWU31_001839"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWU31_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL1084356.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="PWU31_001840"
     CDS             complement(14993..15553)
                     /locus_tag="PWU31_001840"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL1084357.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="PWU31_001841"
     CDS             15906..16880
                     /locus_tag="PWU31_001841"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL1084358.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="PWU31_001842"
     CDS             17050..18450
                     /locus_tag="PWU31_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL1084359.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="PWU31_001843"
     CDS             complement(18528..19250)
                     /locus_tag="PWU31_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084360.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="PWU31_001844"
     CDS             complement(19329..19835)
                     /locus_tag="PWU31_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1084361.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="PWU31_001845"
     CDS             19958..21757
                     /locus_tag="PWU31_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL1084362.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23399)
                     /locus_tag="PWU31_001846"
     CDS             complement(21825..23399)
                     /locus_tag="PWU31_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1084363.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6481 a         5109 c         4297 g         7783 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    22501 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22561 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22621 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22681 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22741 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22801 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22861 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22921 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22981 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    23041 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    23101 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    23161 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    23221 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    23281 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    23341 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    23401 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    23461 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    23521 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23581 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23641 ataaaaaaag aaattattca tcactgaata
//
LOCUS       DANJMA010000039        23464 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000039 DANJMA010000000
VERSION     DANJMA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(273..2459)
                     /locus_tag="PWU31_001847"
     CDS             complement(273..2459)
                     /locus_tag="PWU31_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL1084364.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2677..3360
                     /locus_tag="PWU31_001848"
     CDS             2677..3360
                     /locus_tag="PWU31_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL1084365.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3441..4160)
                     /locus_tag="PWU31_001849"
     CDS             complement(3441..4160)
                     /locus_tag="PWU31_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1084366.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4472..4678)
                     /locus_tag="PWU31_001850"
     CDS             complement(4472..4678)
                     /locus_tag="PWU31_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084367.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4922..5524
                     /locus_tag="PWU31_001851"
     CDS             4922..5524
                     /locus_tag="PWU31_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL1084368.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5665..6945
                     /locus_tag="PWU31_001852"
     CDS             5665..6945
                     /locus_tag="PWU31_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1084369.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6958..7686
                     /locus_tag="PWU31_001853"
     CDS             6958..7686
                     /locus_tag="PWU31_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084370.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7755..9278)
                     /locus_tag="PWU31_001854"
     CDS             complement(7755..9278)
                     /locus_tag="PWU31_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL1084371.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9530..10711
                     /locus_tag="PWU31_001855"
     CDS             9530..10711
                     /locus_tag="PWU31_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1084372.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10771..12399)
                     /gene="treC"
                     /locus_tag="PWU31_001856"
     CDS             complement(10771..12399)
                     /gene="treC"
                     /locus_tag="PWU31_001856"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL1084373.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12796..15507)
                     /locus_tag="PWU31_001857"
     CDS             complement(12796..15507)
                     /locus_tag="PWU31_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084374.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15517..16218)
                     /locus_tag="PWU31_001858"
     CDS             complement(15517..16218)
                     /locus_tag="PWU31_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084375.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16404..17003
                     /locus_tag="PWU31_001859"
     CDS             16404..17003
                     /locus_tag="PWU31_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1084376.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17084..18097
                     /locus_tag="PWU31_001860"
     CDS             17084..18097
                     /locus_tag="PWU31_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1084377.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18205..19122
                     /gene="rbsK"
                     /locus_tag="PWU31_001861"
     CDS             18205..19122
                     /gene="rbsK"
                     /locus_tag="PWU31_001861"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL1084378.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19150..19545
                     /gene="rbsD"
                     /locus_tag="PWU31_001862"
     CDS             19150..19545
                     /gene="rbsD"
                     /locus_tag="PWU31_001862"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL1084379.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19545..20918
                     /locus_tag="PWU31_001863"
     CDS             19545..20918
                     /locus_tag="PWU31_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1084380.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21275..23419
                     /locus_tag="PWU31_001864"
     CDS             21275..23419
                     /locus_tag="PWU31_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1084381.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         6844 a         4592 c         4217 g         7811 t
ORIGIN      
        1 tataaaaaaa aatcaaaata actgattatt tacgtttgaa taatgagaat ttttctatat
       61 attctctatt ttgataatag tattctcatt ttcaaaataa ataagggagg tgtgacaaaa
      121 ttcttgtcac acctcccttt tccgaataaa ctgtgttcca gaaatagctt cttcgaaaat
      181 aagctgaaat ttcacaaaaa tttgagaagc aattttcgta cattccttct tctttcttga
      241 aactgaccac ttctgtcaca ccctctcttc tatcactttc cttttttcac acgactcccg
      301 gtttctttga taaatctcga tactttcaat tttcttgaat ttttcatttg aggtacacat
      361 agatacagac gatttcttgc tctagtcatc gcaacataaa atagtcttcg ttcttcttct
      421 atcgtcgtca ctggttctgc ttttatttca ttaaagtcga ttactttctt ttctgtcgct
      481 ccgttataag gcaaacttcc ttcgttacaa ccaatcaaaa agacatttcg gtaactaaga
      541 cctttacttt tgtgaatgct ctggatacat agtgcatcaa gttttccttg tctgtaagca
      601 atcaagcggg tttttaattc tttagctgtc gcttgggttt cttgcgataa ggcttggagt
      661 cttctgaaag tagcatgctg ctttacttct tctcgtatcc attcttgatc actctggtcg
      721 atttccttag ctatacgtgg cagatgaaga tagctttgaa caagcgaata gatttcttga
      781 tgctggtctg ttttacgcaa cgcttcctca aactctcgga tttctgcaga tttcgatttt
      841 aaattggggc ttatcgacaa aatataatct gcaacggcta tattttgata tttaggagaa
      901 aagtattgat cgcattcact ttttttcagg tagcagtctg ccatcagctg ccggagcaat
      961 tgctggtagg cctgattttt cttttgcaga ttcagtgctg tttgccaatg aacaattttt
     1021 tgatagaaat gttggaaaga cttgatacca aagatacctt catatggact atctgcttcc
     1081 agtacaaaag gaatattcgt tttcagcaga gattggacaa gctgctgcgt ttgtgaagct
     1141 gatcggtata atatgattga ttccttatat ttcccatttt tttgaataaa tgtctgaatt
     1201 tgtttgatta gccactgact ttctgcttta gtatcagaaa acaaataggc ttccataaca
     1261 cctgcttctt tttgtgctgg aatcatcgcc tttggtatcc gatcattttt attatagctg
     1321 atcaatctat ttgctccttg gacaatagct gcccgagaac gataattagt taataagaaa
     1381 agagttttgg taggatattc tttttcaaag ttcttgatgt atgctggctc tgatcctctg
     1441 aatccataga tactctgatc atcatctcca acaacaatca acttgatcct gttttccttt
     1501 gtcatccgtt tcacgatttc ccattgaagt ggattagtat cttgaaattc atcaatgaat
     1561 agataatcat atttttggcc gatataatgc tggatctcct cattctccaa agcgcttttc
     1621 agattttgga gtacatcatt gaactctagc aattgctggc ttcttttttg ttcttggagt
     1681 tccgtgagca atccatacag tcgttctgat atcggctgtc gtgcattctt atcaatggaa
     1741 tcatattttt ctttaactaa ctgaaccatt tcttctactg aaaggttcat attctgccaa
     1801 tagtctaacc gagaagcaat cgttttttct ccatattgat caaatatata aagattatat
     1861 ttacgtatca gctgttgcaa aacagcattc actttttgtg cagtatcctt tgaaaagtct
     1921 ccttgtattc cttgcattgc tacttcagga aattttcgac aaatttcatt gtatatccta
     1981 aaaaagatac tatggaatgt tccgatatgg acagcggttt ttctctggct gaaattatct
     2041 actcgttttt ttagttcatt tgcagcgttc cttgtaaaag tgatcattag tatgcgttct
     2101 tcgtctactt gtttttgcgt catcagataa acagcttttg cacagattgt ttgcgttttg
     2161 ccgctcccag ctcctgctat gattcgaaga tgtttcttca tatcatatgt gatagctgct
     2221 ttttgttctt gattgaagcg attataggaa cgtttctcca tcattttaaa gaaagagctg
     2281 tttccttcac tttcttttac ttgtacttct tcctcaataa gtccgggtgc ttcgccataa
     2341 gccgcttcat aggtagcaat gatttcttct tgtagccgaa agacttgttg aagcttttta
     2401 tacttgttct cccagcgttt caattctttt tgtaatcgga gaatttcttt ttcattcaac
     2461 tgattgattt ccttcccatc tttcttcttt gaatttgttt atttcaagtt ggttatttaa
     2521 agtttcgttg tctatttttt ctttatacta acagaccttt ctaaaaatga aaatgggaag
     2581 cgatatgatt ttagttttct gaaaattttt cctttttttc aatcgtttat gctaagataa
     2641 ggaagacaaa aatattcata aaaggtgatg aaaaaaatgc agacttcctt tcacgagggg
     2701 cgtacaatag gacttttgct gacatttatc ggaggagcca tggacagtta tacttatatc
     2761 caatatggtg cttttgcctc tgctcaaact ggcaatatca ttctatcgat catccaagca
     2821 tttgacggag aatggctaag tgttggtaaa aaagtattgt ctaccctttt ctttttctta
     2881 gggattcttc taacaaagta tctaattgat tattttagaa aaaaagaaaa acatttctgg
     2941 cgcttgtttg ttctatatta tgaagcagtg atttttttct ttgttagttt gacacccgtc
     3001 catgagcatc ccgcattagt aacgattttg attgctttta ctgcatcgat ccagtgggta
     3061 gcttttgata aaatcaacgg gcgtgcctac actaatttat ttacaactgg aaatttaaaa
     3121 ggggtagcaa cgaacttata tgattattgt atttctaaag aaaaagcaga ttttgatcga
     3181 tttttccact atctaagtgt cgttcttgcc tttatcgcag gagctattgt ccttgttttc
     3241 tgtcatcatc tgtttggcgc ccattcgatt ttgatcgttt ccattttatt tttgatcatg
     3301 gccctcactc agacatttca tttatggcaa ttttatcata cgaatcggtt cactatttga
     3361 tctcaatgaa tcaaaaaaac taagcaagat gctgctcaca gagaaatatc tgcgagtgag
     3421 caatttgctt agtttttata ttatttagtt gtaaagattc cctttgcttt cgggttattt
     3481 gtagtgtagc ggatcgactg ggcatctgcc tcttctgtca ttttctttgc ccgcatcttg
     3541 atttcttgta cgatttgttc tccacatgcc ggatcggcta cgaaatcata tttgtcccca
     3601 tcgatcacta ttttgggaca tatatcaaac tgttcatacc actcttcgta tcgtcgattg
     3661 agttctttat agtaggaata gagttcagga tcttgttcta gctgttcata ttcccggcct
     3721 ctttttttga ttctcgctaa catagtttca aaggatacac gaatataaac cagtagatct
     3781 ggtttctttt gtggcgaaat ttcttctaac tccttcagca tcgtttctaa aagaccttca
     3841 tactgcttta cttctacttc agaaacacgc cctaaatctg cattcaaatg aaacagcata
     3901 ctatcttcgt agatggagcg gtctagtacg ttgtcatcat ggctgaaagc atctttgatt
     3961 gccaaaaagc gtttgttcaa aaagaaaatc tgtaataaaa aagtatactt ttctggatta
     4021 gaataaaata gcggcaatac ttcattatct tccacgttct cataaaaagg ttttgtttct
     4081 aaatcttggg caagcatttc agttagtgtg cttttccctg caccgatcgt tcctgcaaca
     4141 atgatcacaa ttatcggcat ccttcctgta ataaaatctg ttcttcattt tactacttca
     4201 tttatagaaa taaaagcctt gactttatct gctccttaaa agctacttct ctaaaagtag
     4261 ccaaaagtaa ctcctattca tgaaaataaa ggctgtgaca aaagtcttgt cgcagccctt
     4321 ttttcaaata aacagtattc tcctgccaac acctcggtca caaggcaata ttcataaaac
     4381 atgaaaagct gttttttaac attattcctt gtaactcaga gctaaacggg agactcacaa
     4441 cctctttatg ctctcaacat tttatctgtt tttattcttc tattgtactt gatttgcgta
     4501 caactaactg ccagtgcaga ttatgagcgg tatcattgtc ttctactgcg catttcatga
     4561 cacgtatcat cacactattt tcatagattt tttctatttt tccttttatt ggtgattcaa
     4621 ataaatcaga tttaaatgca tatgtttcgc cgacttcaaa caattgttca ttttccataa
     4681 gaatccctcc atcacctttt ataaggtcag taatttttta acataaatca ctcgaaaaag
     4741 cacaaacttg tcataaaaag tacttttcgt atcaaaactg tcactttttt tggtaaggtt
     4801 cttggaaaac gaagattttt ttaggagatc atgacggttt tgatgtcaaa accacttttt
     4861 atgaaacaaa agacgttttt tattttttta gcataaaatt tgtggtggag gtacgtatga
     4921 gatgaaagat aaagttgtga acaaattatt agaagcctta ttcagcggag acaaatcgga
     4981 agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa gttttattga ataatcttgg
     5041 aaaactagcg gtcgttctag tcttttctct ggtgacaggc tcatgggcgg aaacaggaat
     5101 cacctttctt tcttatatct gcatccgcag atatgcttat ggattgcatt ccgactctga
     5161 attcgtttgt ctgctgtgga cactacttta tttatggggc gttcccttgg tgatgaaaca
     5221 tctgcaattg actatctcct tcccaatgat ggtctttttg ttaatcagct gctttttgtt
     5281 actgcttcgc tatggatcaa gaggtacagc aatcaatccc attgaacccg agaaacgtcc
     5341 cccattatta aagaaggcga tcagcatgtt tctaattttt tctctcatta ctttattttt
     5401 ctcagcttct tacttttcta cctatttgct acttgggatt gttttagaaa tagctacgtt
     5461 gcttcctatt acaaactact tgatgaattt tggaggaatc tttcatgaaa aaaacaatca
     5521 ttaaacaatt aactaactta tctgactttt ttgtatctgg ctgctgcttt ggtatgagtt
     5581 ctgaattcaa aatccctgaa aaattaaaaa aataatacgc taattgtgct ataatttggt
     5641 aaaaatcaat gggagctgta ctttatgttt ttatggtcca tagcgttgca atatttgatt
     5701 tacacaattt gtatcttatt gattgaccat cacatccgat acaaatatct gtttgcggga
     5761 gtcattctga tttcttgtat tttgattgga tgggtctatt cgattttagg gatttttact
     5821 gctctaattg ctataggaat gctgatgctt acttttttct tcttttcgac ttcgcgtcaa
     5881 caattggttt acgcgcttcc ttttgggtta ttgacgtcgc ttttaggaga tcacttaaca
     5941 agcatcggcg atttttttgt tttgaaatct tcggttattg aaccaggaga ttcattgcag
     6001 tattttcaca ttgtcttatc cgctttttta tctgctttat tagctttttg tttaaaaaaa
     6061 tcgttagaga cttggtttca cactgtggac cgccgtatga tcggtacgat cgggacattg
     6121 atattattta catactatat cattattttt tatacccgtt tttctggtga aacacctgat
     6181 gtcttagctt tgaatacgag ttttttcatt ctatatctgt gtgttggcct gatcatcttg
     6241 gtttattatt ggaaaatctc taataagaaa ttagctgatc agttattaga gcaacaagtc
     6301 aggatgcaac aggattatat caaagatcta gaaaaaaact atcaagactt gagagaattc
     6361 aaacatgatt accaaaatct gcttttttct cttcacagct atatttcaga aggcgactta
     6421 gaaggattac ggtcttatta tgatcaaacg atattaccta cacgccagat gatgaacctt
     6481 tttccggcca atctaagtat gttggataat atgaaagttc ctgagatccg tagtttactg
     6541 tcattaaaac tcatgatggc acaggaaaaa gggttgatcg tccagcttca ttttcctgag
     6601 ccaatcgaac tggatacgaa acatacggta aaccttgtac gtatgcttgg tattatcttg
     6661 gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa aaatcgaatt gaccatactc
     6721 aagaagaatc attccgtgat gattcgtgtg atcaatacaa caacaaatac attaccgtta
     6781 aaccagttga aacaaaaagg gttctcaaca aaaacaaatc caaaaaacga aggccttggc
     6841 ttgtttatct tggatgaatt gacaaagacc aatccttatc ttttccttga aacatcaatt
     6901 gaaaaccagc gtttttctca aacgatttat atacaaataa aataaaggag taatgatatg
     6961 attcccattt atatttgtga agacgatcca aaacaattag aagttttatc aacggtgatc
     7021 aaaaatagaa tcatgattga aaacttagac agttatgtcc atatttctac ctctgatccg
     7081 gaagaacttc tcaatgcagc caagatcaga acttcttctt ttggtattta ctttttagat
     7141 atcgaactag aagacagtga aatggatggt gtagcgatcg gtaaattgat ccgtgaatta
     7201 gatcctttag gaaaaataat ctatgtgact tcccatacgg aaatgtcgat gaaaatctta
     7261 aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag atctttatga tttaaaagaa
     7321 aatgtcgagc atatcctgag caaaattttc gaagattttc aaatgtccca aatccaaaaa
     7381 gatatcttca aaatagagtt taatgatgag atcaaatttt taccgatcaa agacatccaa
     7441 tatttttcta cttcgctagg agcgccacat aaactggaag tccatttgac tcatgcccaa
     7501 ttacaattct atgagaagat caaagaaatc gagaaaatgc atcggaattt tatccgttgt
     7561 cataaatcct atgttatcaa tacacaaaat gtgcggtcga ttaataaaaa aaccagagaa
     7621 gtcacgttgg caaacggcga gaccatccca gcctctatcc gtggattaaa gaaattgatt
     7681 tcataaaaag aaccgaatag tttggaccga ctaaatgacc agccgatcca aacccttcgg
     7741 ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa tttcacgtcc atccattgta
     7801 tctggataaa atgttggcca attctccatt tctttcagca atacttcttg actttcattt
     7861 cctgcatata aatgaaaatg ttcggagtca gccggtgcaa tcgtgtgatc actgaattga
     7921 atatatctaa aagcgccact ttccttatct tctgcttcga atagatacct tacgccacgg
     7981 tttcctgcct cgtaggtcaa gatttctttc cctacataac gataatttga acgtttcgtt
     8041 gttccatcct taaacgtaaa tgccatctct ttctcactga tcttgattga agcaatatct
     8101 gtttgatagc ccttcgtata atattcttta tattcttgtg ctgtcttatc ctgattcaat
     8161 ttttctttat attcaaaaac aggatcaaaa gtcccatctt ctacatacgt atctactgtt
     8221 tgccaagttc ctgcccaatc tgaaagagag cgatcttgta catcctcgtc ttcaaaataa
     8281 ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat cttctagcgg gttttctgtc
     8341 tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga tagaaagata gttttctcca
     8401 ttttctattt gttcattcgt caatccttcc aaaggattca atacttctaa agatacacct
     8461 gcttcgtttg ctaacgttct ggcaatacta tctttggcgt tttcttcaaa ataaatatag
     8521 ttgattccat gttctttaac aaagtgtttc agttcagcaa ttctcgccgc agacggttct
     8581 tcactagaag aaagaccagc aatcgatact tgattcaatc catattctaa cgctagatag
     8641 ttgaatgcag catgttgcgt gacaaaagat ctttgttttg cttgcttaaa ggtttcttga
     8701 taaagctgat ggagtgcttg aagtttctcg gtataggctg ctgcattttt tgtccatact
     8761 tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta tgtttgctac ctgcttgatt
     8821 gctaaacttg gagctagcca tacatgtggg tctagctcat gtgtatggcc ttcttccccg
     8881 tgttcatggt catgatcatg tccttcttca cttcccggaa gtaaaagcat accttctgtc
     8941 gcttttatcg tatggacatt gccttcttgt aatgtttttt ggattgcggg gacccatgtt
     9001 tccatgtttt catcattata aacaaagata tctgtatctt ggatctttgc catgtcttta
     9061 gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa tcaataactc tacttctcct
     9121 tcatccccga cgattgcttt tgtaaaatca tacatgggat aaaaggtcgt catcacgctc
     9181 aatttttctt tactgtcctc ggctttccca cttgccccgc aagcagttaa catacctgct
     9241 aaaaccagca tggccaatat acctatcaat cgtttcattt atgacactcc tcttctcatc
     9301 ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg taatagttac gttttagcaa
     9361 caggatttaa tttatcatat ggttaaaaga actgtcaagc gttttcttat tttaattgat
     9421 gagaaagttt atatgaatga ttgacgatat ttttgcaatc aacctctatc attttccttt
     9481 tatacccgct tttgtctata atgaagttat agataaagga ggaaaaagag tgaaaaaaga
     9541 aacagataca acgaatcgtt tgatccgctt tttaggagga aaaacctctt actatgtttt
     9601 aggaatagtt atcttacttg cgctgatcat ttattttttc catcagatct cgtttatatt
     9661 caagcctgtc ggaatcatta tcgctactat cttgccccca ttattgtttg cactgatctt
     9721 gtattatctt ttggaaccag tcatcaaacg tttgagcaag cacctttcga ggaacttgtc
     9781 agtcattatc gtttatgtga ttattttatt tttattgggg cttggaagca tatggcttat
     9841 tccttttctt gaagaacagg caaagacatt gatcagtagt ttgccagatt tgtttcaaga
     9901 ttttcaagag agtctccgac aatttttgga gaaaacacct tttgccgatt catttaatca
     9961 atttttcgct tctattgacg acgtgacgtc tgatatagct ggatttattg gaaattattg
    10021 ggaaagtggc gcacaacgga tcagcaacat tttttcaact gttacagcta tttttattac
    10081 tttatttact ggtccaattg tcgctttttt ccttttgaga gatcctcaaa aattttatca
    10141 gtcagtttta gcgattgtcc caccagcatt tcgtacagat tttaaaaatt tgacaaagat
    10201 tgctaatcaa caattgggtt catttctaaa ggggcaaatc attgcttcat ttatccttgg
    10261 agcagtctac tgggtttgtt tcttactgat cggattagaa tttgctagtg ttttagctat
    10321 ttctgctggg ttgttatgta tcattccata tattgggccg tttatcgcct tctttccagg
    10381 tttgatcatt gcattccaag actctacttt tatggctgtc aaatttgtca ttgtctggtt
    10441 tgccgtacaa cttctccacg gtgacttggt gattcctcga gtaatgggtg atcgtttaca
    10501 gatccatccg attacgatct taatcgtttt acttgtcatg ggtgatctaa tgggaattgt
    10561 gggcgttatt tttggtattc ctatctatac gttagtcaaa ttactcgtta cttttatttt
    10621 ccgtaaattc aaacaacgct acaataaatt ttatggcgat aaaggagagt atgaaaactc
    10681 ttcattttct gaagaagatt attttaaata aaacacaaaa cgtcgatctt acgagaatag
    10741 ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc agcaaagcaa ttgcctggta
    10801 tggttttagc tccagacttt gatgaagaac agtatcggaa taattatcga taagaatatt
    10861 ggcttctgtg aatccttctg ggatggcgat ccttgtatct ttatcataga aatttgttaa
    10921 tacaagcagc tgctgtcctt caaattggcg aagaaagcca taaacttccg gatgatttgc
    10981 ttcataagca tgataatctc cttgtgcaac gattggatat gtttttctca gccgaatcaa
    11041 tttcttgtaa taggacaaaa tagaatcttc agaagatagt tctttttcta cattaatctc
    11101 ttctagcttg cctccaactt ttaaccaagg tttccctgta gtaaatcctg cttgttctgt
    11161 gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat tttgctttga tccgacgaaa
    11221 cgcttctttt tctgtgaatc cttgttcaag gagtaactta taagcattca aactttctac
    11281 gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc atgccgattt cttctcccat
    11341 ataaatatag ggcgtcccac ggttcaaatg gatcatggtt gctagcatct ctgctccttg
    11401 ttttcgatac tgatcgatag ctacaaatcg attgagcgca cgtggctgat catgattatt
    11461 aaaaaataga gcattccagc cgttaccctc actcatcttt tctccccatg tatgaaatag
    11521 tcgctttaat tctgcaaaat caaatggagg agtagaccat ttattcccat tttcataatc
    11581 gactttcaaa tgatggaaat taaagaccat tgataattct tctctactag gttctgtata
    11641 caagatacaa ttttcgatcg tcgtagagct catttcaccg actgtgatga tagtggaatc
    11701 cgtcccaaac gttgcttggt tcaattcttt tagatacgta tgggtgattg gtttgtccgt
    11761 atattcaggt ttgccatcat tttctttatt atttttcaat tgttcgtctt taccgataac
    11821 attgataaca tcgaaacgga atccttttac ccctttttcc atccaaaaac gaacaacttt
    11881 gaatagttcc tctcttactg cttggttccg ccagttcaag tcagcttgtg tccgatcata
    11941 taagtgcaaa taatatttcc ccgtatctcc aaatggcgac caagcacttc caccaaattt
    12001 tgatacccaa tccgttggct cttctcttaa aatgaagaag tcctgataat gctggtcccc
    12061 tgctagtgct tttttgaacc attcatgttc tgtcgaaacg tgattcagca ccatatcgag
    12121 catgatttct atccggtatt tctttgcttc ggctattagc tgttcgaatt cctccatcgt
    12181 accaaagata ggatcgatgg ctgtgtaatc agagatatca taaccgttgt ctttttgagg
    12241 gcttggataa atagggttta gccaaatcat gtcgatgcct agatcattca gatacgggag
    12301 tttttctttg attcctttta aatctccaat tccatcacca ttactgtctt tgtaagattt
    12361 gggatagatt tggtaaatga ctttgtcttt aaatgtcatg gatatttacc tccattcaac
    12421 tgatttatta cctaccacaa cttatatata caagttatca tttattatta taactgaacc
    12481 gaaagcgttt gtaaaattta tctttataca ttctaagagg tctgttgccc aataaaaaac
    12541 aagagatcag acatgtattt gtctatcctc ttgtttaaaa tagcaaaata ttcttgtttc
    12601 ttctataaat ggtgttcaaa agcttttatc tacggcaatc agtcttctca aatgtaaaac
    12661 atgaggagct aattttattt gagtattctt tttgctcgat gcaggacggc ttttttacaa
    12721 cctctgattc atggtgttca attagcagct gaccctcgtt attagctcaa aacgctgatt
    12781 tcacaacctc tgtttttatt cattcgattt caacgcatca atcatatcga tatgctttaa
    12841 tttgaaatgg gtaacaatca tcacaataac cgtaaatacg atagttaatc ctgctgataa
    12901 gacataagcc caccatgtga tcactaatgg aaagatgaca ttctccatag acgcttgttg
    12961 caagatgaag tcagtcaata tatatcctac accaaatcct ccgatgattc ctaaaagagt
    13021 aaagatgata ttttctcgaa caatgtacat cgtgacttct ttatcaaaaa atccaaggac
    13081 ttttattgta gataattcgc gtacacgctc ggaaatattg atattagtta gattatacaa
    13141 gacgataaaa gccagcagtc ctgataatac gacaaagatt aagacaaccg agtctaagtt
    13201 cgccattgat tcttcttgcg tttcgatttg tttggacatg aaagacgtat ttgtcacagc
    13261 gtctgtggca agcagtttct cggaaagcat ttcttcttct tttgttgtca tcttatctgt
    13321 acgcagcaaa aatgcattat ccatcatcga ttgatcagta actgaagcat aatattcagg
    13381 ggacatataa ataaagctac ctagatagtt tttcacgatt tcttttactt cgactttcca
    13441 ttctttttga tcttcatcat aaagcgaaat atgatctcct gtagataagt caaatagttc
    13501 tgctactttt tgcgtaatag cgattcctgt tttaggcaag gagcgtgggt tctcttcaag
    13561 atcagttaaa tggataaaac gatccaaatc agctgtttta cttggtacga tcactgtcag
    13621 actttgcgaa gcatgtcctt tttttctcaa ttctacggtt tgagtatgaa tagctaggat
    13681 ctcatttact ttttcttgct tctgcagcat atccgctgct ttttgacgac tatcttccgt
    13741 ggatgaatcc aatgtaacga ttgcttgata gtcaatgatc ggtccaaatt gtttggctga
    13801 aacagaactt agagaatctt tcaatcccac gcctgcaacc atcagcccgg cacatccagc
    13861 aattccgata atcgccataa gcattcttga cttatatcgg aagagatttc ggtaactgat
    13921 tttttgatta aagctaaggc gtgaccacag cggagtgatg tattcaagaa aaatgcgttt
    13981 ccctggcttc ggtgctttcg gttgaagcaa tgcagcaggc cgttctctta aatctttgaa
    14041 taaaacgatc attgcagcgc caaatgcggc aatgaagaat gcggcagctg cttgtaaaat
    14101 cggcggccag tcataaaaaa taagcgcact cccaatatca taccgctcgt tagacagctc
    14161 aaaaatgatc cttggcaaaa gattcgttcc taatatcgtt cctaaaacaa tacctatccc
    14221 tgaggctaga cttgcataga ttgcatattt ttttgcgatt tcaactttcg tataacctaa
    14281 cgcctttaat gtcccgattt ccctgcggtt ttcttctacc atacgtgtca ttgtcgtaaa
    14341 ggtgatcaat gcggcaataa agaaaaagaa aacaggaaaa acattggcaa tcgcagcgat
    14401 acgatcagac agatcaccaa attcttgaaa accgacattt tctgaacgtt cattgtatag
    14461 gtatgatggt ttagttaaat tttcaatttt ttctgcattt cgattcaatt cttcctctgc
    14521 agaggccagt tcacgttctt tctcaactaa ttgcctttct tgttcagcaa gttgagcttc
    14581 ttgttgagat aatgtttgaa cagcgattgt tcgttgcgct tcaggaagct gctggatagt
    14641 tgctgactgt tgcgctaact gttttttagc atcttctaac cgtgttcttg cttgcgttgt
    14701 ttgtgctttg ccatccgcta ttttttgctg tgccggttct aattcttcgt tggccgattt
    14761 tttcagctct tctaagcgtt cttcgggtcg atcttgtaaa tacttttctg ccttttcttg
    14821 attttgctcc attttatctt tgtaagcttt gctgtacgta ttcaaattct ttacgtttgt
    14881 aaaatcaaga tataagacag aataaatatc tgttttaaaa acggattcag gtaaatagac
    14941 aaaataatcg acgctaccat ttccgacgtt cgcgtatcct ctctccaaat tgttgatgaa
    15001 caatggagaa cggacaaagc ccgtgatggt atactctttt tctttcaatt catctgaatc
    15061 aatctgataa atatcaccga ttttataccc ttcatcttcc gctttttcat ccaatacaag
    15121 ctggtttggt ttttccggga gttttccatc agttaattgt agcttgttct gcttgttatt
    15181 tttgttataa gaaaatattt gaatcacttc gtttttgcca gtattggaat aatagatctg
    15241 ttttcctgtt tctacttttg catctgggat cttttctgct tgagcaatat ctttttgtgt
    15301 caagccacca gtagaaacga tttgcaaatc agaaagtcct gctgtaccaa catagtgatc
    15361 catcgttttt tgcatgacag gtccagcagt tttgacacct acaaataaaa gtgttcctag
    15421 catgacaata aggatgattg caatgaatcg tcctttagat tgccgaattt cgcgaaaacc
    15481 tgctttaaga tatgttcttg ttttcataaa atccccctac catacaatct catcaatgga
    15541 tgaaggatga tcatttaatt caacagacct tactttagca tcattaatgt gaatgactct
    15601 atcagcgatg ggcgctaaag cagaattatg tgtaatgatc aaaacggtat tgccgtgttg
    15661 tctgctagcg ttttgcaata atttcaatac ttgcttgccc gtttcaaaat ccagcgcgcc
    15721 tgtcggttca tcacataaaa gaagtttggg atttttagct agtgcacgag caattgaaac
    15781 cctctgctgt tcgcctcctg ataattggga cggaaagtta ttcaatcgat gagctaggcc
    15841 cacttgcctc aatacctcta caggatcaag cgcatcagga gaaacttccg ttgccaattc
    15901 cacattttct tttgctgtta gattaggaac gagattgtag aattggaaaa cgaaccctac
    15961 atctgttcgt cggtaagcag taagctgttt atctgaaaat tgcgcaatat ctgtgtcatc
    16021 aataatgatc tgaccttcat caggtgtatc catcccacct aaaatattaa gaatcgttga
    16081 ctttcctgct ccacttggac caagtataat cactaattcg cctttttcaa ttgaaaaaga
    16141 aatatcgtca tttgctgtaa tggtcgtttc tcccattttg tatcgtttgt attcattttt
    16201 tacttcgaca tacttcactc tgattcctcc attttcacga tatttctcgc tatatctatg
    16261 catcgaatgc gttttctcac acttatttat tttaccactg aatttctcta gactaaagaa
    16321 aggaatcctt taatttagtt gacaaataaa cgatttatga tttacttaca taaaaaacat
    16381 tgttatttga aagaaggaac atcatgaatg atttaaagga aaatatgatt tttgatttat
    16441 caaaaactgc ttttgtcgcg atcgacatgc aaaaagagat cgtaaataac ggtacactct
    16501 ctccacacac tgcagcatct attctgacaa acaatgattt gttagtaaga acattgaaac
    16561 gtaccgctgc cttgaaagtt ttagtgaacg tagacatctc cactttccct tatttgagtc
    16621 aacaaactga tatgggtgga atgactgctt ctgtacctcc agaattcact gatttacttt
    16681 tgaaggattc tttgaaagat acagataata tgttgactat cacaaaatac aatccaagtg
    16741 ctttttttgg tacttctctt gatcttcaac ttcgccggcg tggaatcgaa actatcattt
    16801 taagcggtgt agcgacaaca aacggtgtat atgctactgc attagatgcc tttcaacacg
    16861 ggtaccatat cgtgcttgct gaagatgctt gcagtgatcg tgacaaagaa tcccatcaat
    16921 tgttcataaa aaagatattt ccgaaaactg cacgagtacg ctcaacgaaa caaataatcg
    16981 aagctatcca acaaagcaaa taagtactgg cacacgtgat tttcatttac ttctttttga
    17041 tttcatggga aaataaccac aggatttaga taggagtatg aacatggtga aaaaaaagat
    17101 cacaatcaaa gaagtcgcta aactttccgg cgtttcaatc acaactgtct ccttgatatt
    17161 aaatggaaat acggagaaat tcagtccgaa aactgttcag aaagttttag ctgccaaaga
    17221 agaattaggc tatcagcctg actattttgc tagacaaatg attaccaagg aaacgaaaac
    17281 aattggtgta ctcgttcctg atatcacgaa tccattcttc agtacactga tgcgaggaat
    17341 tgaggatatc ttgtataagc agaatttcgt cacgatatta tgtaatgctg attctgatca
    17401 tcaaaaagaa atcgaatatc tagccgagct cacacgtcga ggtgtggatg gattcattat
    17461 cgcgacttct gctgtctcta cagatgcaat caacgaaaat ttgaaaaaac aaggccggcc
    17521 cttcattgtt ttggatcaga aaaaatctga aggatttagt gatgccgtac gaacagacga
    17581 ttttcgcggt ggttacctag cgggcatgca cttgcttagt ctaggacatc aaacgatcgc
    17641 ccttgtctat cctgaaaatc caccggaaaa tgtccacgct cggattgagg ggtttaaatc
    17701 tgctcttgat gtttaccaaa tccctcacga ccaactgatc ctcttaccta cacagttctc
    17761 aaaacaaggt ggctaccaga tcacagcaga acttcttgat tctgctgcaa ctggtgtgtt
    17821 tgctttaaat gatgaattag cttttggatt atatcgcgga ttagaggaag ctggaaaaag
    17881 catccctgaa gactacagca ttattggcta tgataatata gatatgtgcg agtatatcaa
    17941 gccgaaactg acaacgatcg cacaaccaat cttcgaattg ggacaaacat ctgccaagtt
    18001 attgttagat cggatccaat tcccagaaaa agaatgggaa gaaaaaagat tacctgtacg
    18061 attagaaaaa agattttcaa ctgcaccctt gaaatagctt tcaaggtgtg ctatattttt
    18121 atcgaactat taaaacgttt tactaaaacg ttttaaaaaa taattttccc atcaaaataa
    18181 atgagatagt tggaggtttg taaaatgaac acggttacag taattggaag tattaatatg
    18241 gacactaccc ttcgtttaac caacatgccc aagccaggcg aaacgatgca tgcgcatgag
    18301 atttttcacg ctggcggagg taaaggtgcc aaccaagcag tggcagccaa acgttcaggc
    18361 gcacggacat cttttattgg aggcgttggg gctgacagcg aaggacaaca gttattagat
    18421 ctactgacaa aagaaaacat cgatacatct gggatagctg aaatccaagg cgccacaacc
    18481 ggacaagcaa tgattatggt agacgcagca ggagaaaata gtatcttgat ccatgcaggg
    18541 gcaaataatg ctttccatga acaagaagtc ctaaaaaaca agcaactgat tacaaacagt
    18601 gactttatta ttgctcagtt tgaaagtagc ttagatgcga cgatcctagc tttttctatt
    18661 gcaaaagacg caggaaaaac aacgatcctg aatcctgcac cagctcgtga aacgattcct
    18721 actgaactac ttgaaaagac agatatcatt ataccaaatg aaacagaaac agaaatcatt
    18781 acaggaatca gagtgacaga tcataacagt ttggtcgcag cagccgaaaa actccatgaa
    18841 ttaggtattg gaacagtaat cattacacta ggcagtgccg gcgctttcta tcatacagaa
    18901 aaagaacacg gtatcgtccc tgcttttaaa gtggatgccg ttgacacaac agccgcaggt
    18961 gatacattta ttggcgcatt aagcagcaca cttcagccag accttagtaa cttgaaagaa
    19021 gcgatccttt atggaaatct agcctcttct gttgctgtgc agtcttatgg tgcgcaacct
    19081 tctatccctt accgcgaagc tttagaacaa tcagcagtct aaaattaaca atgagaggaa
    19141 gaaacaaaca tgaaaaaagg aaaagtaatc aattcagata tttcacgagt aatcgcacaa
    19201 atggggcact ttgataaatt aagtatcggg gatgcaggca tgcctgtccc ctttggtact
    19261 gaaaaaatag atttggctgt ctcaaacggt cttccaagtt ttttatctgt tttagaaaac
    19321 gttttagaag aacttgaggt acaatcgatt gaattggcgg aagaaataaa gacaatgaac
    19381 ccggaaatat tggaaaaaat caaattattg ttaccagata caccgattag ctttatccca
    19441 catgaagaaa tgaaaaaaga gttgaataac tgtcatgcat ttattcgtac aggcgaaatg
    19501 acaccttacg ccaatattat attagtgagt ggagtggtct tctaatggaa acgacaatga
    19561 atcctgaaca gatggtactg tccgagcaga ttcatatgct gattgaacag ttgggaggaa
    19621 aagacaatat tgattctgtc cttccatgtg cgactcggct tcgtatcaaa ttgaaaaaac
    19681 agccagtagc caatgcagaa aaattaaaac agcaaatccc ttccattaag tatttcattt
    19741 tactcgatag atatgcagaa ataatcatgg atcagggatt ggctcctgct tacaaggaat
    19801 tgacgcgtca aatcgatcat tcagctccga aagaaaagct aaatccgatc gaaaaactat
    19861 ttggtttgat atcggaaatc tttacgccga tcattccgct gtttgccggt tctggtatat
    19921 taaaaggtct ggttgtttta gcgactacga ttggctggct tagtgaaaca agcggaactt
    19981 atcatatttt gtctgctgca tcgaatgccg tcttttattt tctgccaatc tttcttgctt
    20041 tttctgctgc caaaacgttt aaagtgaatg gatatattgc ggctgtcatt gctgcagcac
    20101 tgattgaacc gaatctgacc tctttacttt cagacagtgg caaagcagct actgattttc
    20161 tggggattcc tgtagtactg atgcagtata cgtctactgt catgccggcg attttaggta
    20221 ttttctttta ttcttttgtt gaaaaattcc tcaaaaaata tattcgcgaa aatatgcaat
    20281 tagtatttgt acctttactt tcattagtta tcacggttcc tttgacgtta atggtattcg
    20341 ggcctgcggg tgtttacctt ggcgaaggtt tagctgccgc aattacttgg atgatggata
    20401 ttaacggacc gatcagtggc gcaatcatcg ctggtggttg gaatattttg gtaatatttg
    20461 gtatccaatg ggctgtaaac cctgtcatga tcagtaatat ctctgcttat gggttcgacc
    20521 gaatcgttcc gttgacagga gcagccaatt ttggaatggc tggtgcagct ttaggtgtat
    20581 ttctgcgaag taaacgttca aaaacccgtt cgatctccgg ttctgctttt gcttcgattt
    20641 tacttgcggg agtgaccgaa ccaactgttt acggaattgc gatcccattg aaaaaaccat
    20701 ttgttgctgc ttgcattggt gcagctgctg gtggagctgt aatgggattt gcccaagtga
    20761 aagcgatcgc ttttgtattt ggtagtctga ccacattgcc tgcattcatt tccggtactt
    20821 ttttctggta cttagcagga ctggctgtta gtctagtcgt agcaatgatc actactctcg
    20881 ttagcgggtt tgatgaagac ctaatgtctt atgaatagtt aatgaaaaag actgaagtat
    20941 gagatgttta tcacatattt cagtcttttt ctttttagga tcattatttt tataaatttt
    21001 tgtgatactt tccaaacttt ggtataattt acatatgcat aaccattttt atccccactt
    21061 gtttatcccc aaacaaaggt ggtctatgca ataaaatgaa cgtcaaagaa aaacgaatga
    21121 atgctcgttg cttatttaca aattgtttta tttagaatac acagacagat ttatgaaaag
    21181 aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc taatgaatgc tgaataagac
    21241 agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa aataaaaaaa cgatacgttt
    21301 gctagctgct acttggctga ttgctccgtc tgttttgtcg accaataccg tatttgccgt
    21361 agataatcaa atagctgatc aaacagtcac aacagatacg actacatcca gctcagctac
    21421 tagtgaaaca acggattctt ttacagaaga cccaactgga acaactaact caacaacagg
    21481 atcgaccgag ccaactgaaa ttgatccggc tgatccatca gaaaatgaaa agccagccga
    21541 gacatattcc cttacaattg ctccttcttt gattggtaag atccaactga ctgcttcgac
    21601 agcggattcc cctgaacagc aacctttgga tattacggaa agtggaacgg tagaaggttt
    21661 attagcggaa acgaaggtaa cttacactat tacgccagct gaaggtgaac aaattgcttc
    21721 agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca aagaccttta cagtaggtac
    21781 gaacaattta tctattactg cagaatttaa agctggaccg gatacttcaa cagagaaacc
    21841 aactgatcct agtgaagaag tccctagtac accagatccg gaaacgccaa acaaaccaga
    21901 tgtgggtaat aacaacaata ataatggttc aaataattca aacaataata acaataattc
    21961 gaacagcggg aattcttcga atcaaacaaa cccatctgga tcgaataaca atggaaatac
    22021 tcagagagaa ccaaaccatc caaacaatgg ttcatcgaat ggcggaacga acaatgcttc
    22081 atctcgtcca aatacagcaa atcaaagcgg tatagagaat ccatcttctg cttcttctga
    22141 ttttgttgta aaatcaccaa ttgatgctgt attgcctaca aatacaacga atgtccaaca
    22201 agcaatcgtt cgagaagcat tcaagcatct tggaaaacca tatgtatggg gagcaaaagg
    22261 tccaaatact tttgactgtt ctggtttgac ttattacgta tatatgaaag caaccggtca
    22321 ttatatcggt ggctggacag gcgaacagca atatgcaggt acgcagattc cagtaagtca
    22381 ggcccaacct ggcgatctcg tcttctgggg accttcttcc ggtgttactc accatgtagg
    22441 tatctatatt gggaacggac aatttatcca tgcgcctcaa ccaggagata aagttcgagt
    22501 aacttctatt tcagatttca cgcctgattt tgctgtccga gtaaacttag caggtttacc
    22561 tgctgcttca ggttcgctga caggcggctc atctatttta gatggattag atggctcgtt
    22621 ccatttcagt caaaaccaaa caactgatca gttcttgaaa aagattgcag atgatgcaca
    22681 ggaaatcggg caaaaagaag gaatctacgc atcagtcatg atggctcaag ctatccttga
    22741 aagtggatct ggaaacagct tattatcaag cgaaccaaat cataaccttt ttggtatcaa
    22801 aggatcatat aaaggaagca gcgtgacctt caatacactt gaacaagata gttccggaca
    22861 aagctaccaa atccgcgcac aattccgtaa atatccttct tacaaggaaa gtttggaaga
    22921 ctatgccgac ttgatcaaaa atggactgac tggcaatcct gatttctaca aaccaacttg
    22981 gaagtcggaa acaaaagact acaaagaagc aaccaaatat ttggaaggac gttatgctac
    23041 agatcgccaa tattcacaaa aattgaacgc gatcatcgaa gcgtacgacc tgacaaaata
    23101 tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca aacgctgaaa tcctcaatat
    23161 gtctaagcga ttcgatgtgc caatcaaatg gacaacagat ggcgtgccaa tcacaggatt
    23221 agaattttca acgataaaac gcgtcaacaa cgcttcttcc atcttccgtg tcgcttcaat
    23281 ctgggatttg tggaacaatt ttacggcaag acagatacca gaaacaacta ctcgtacagt
    23341 taaagctgca actgttcctg tactaacctt gttaagttta gacaaatcat tctggcttga
    23401 tctgccttta aagaaataga ataaattaaa aaggggatgt gacactgtca cactcccttt
    23461 ttaa
//
LOCUS       DANJMA010000040        23436 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000040 DANJMA010000000
VERSION     DANJMA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            41..1702
                     /locus_tag="PWU31_001865"
     CDS             41..1702
                     /locus_tag="PWU31_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1084382.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1772..2119)
                     /locus_tag="PWU31_001866"
     CDS             complement(1772..2119)
                     /locus_tag="PWU31_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL1084383.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2203..3144
                     /locus_tag="PWU31_001867"
     CDS             2203..3144
                     /locus_tag="PWU31_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084384.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3145..4188
                     /locus_tag="PWU31_001868"
     CDS             3145..4188
                     /locus_tag="PWU31_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084385.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4194..5264
                     /locus_tag="PWU31_001869"
     CDS             4194..5264
                     /locus_tag="PWU31_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084386.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5254..6207
                     /locus_tag="PWU31_001870"
     CDS             5254..6207
                     /locus_tag="PWU31_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1084387.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6219..7229
                     /locus_tag="PWU31_001871"
     CDS             6219..7229
                     /locus_tag="PWU31_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1084388.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7514..8335)
                     /locus_tag="PWU31_001872"
     CDS             complement(7514..8335)
                     /locus_tag="PWU31_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1084389.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8432..8710)
                     /locus_tag="PWU31_001873"
     CDS             complement(8432..8710)
                     /locus_tag="PWU31_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084390.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8978..10987
                     /gene="metG"
                     /locus_tag="PWU31_001874"
     CDS             8978..10987
                     /gene="metG"
                     /locus_tag="PWU31_001874"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL1084391.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11466..12053)
                     /locus_tag="PWU31_001875"
     CDS             complement(11466..12053)
                     /locus_tag="PWU31_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084392.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12228..13550
                     /locus_tag="PWU31_001876"
     CDS             12228..13550
                     /locus_tag="PWU31_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1084393.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13705..14481
                     /locus_tag="PWU31_001877"
     CDS             13705..14481
                     /locus_tag="PWU31_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1084394.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14474..15043
                     /gene="rnmV"
                     /locus_tag="PWU31_001878"
     CDS             14474..15043
                     /gene="rnmV"
                     /locus_tag="PWU31_001878"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL1084395.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15036..15920
                     /gene="rsmA"
                     /locus_tag="PWU31_001879"
     CDS             15036..15920
                     /gene="rsmA"
                     /locus_tag="PWU31_001879"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL1084396.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16371..17000
                     /locus_tag="PWU31_001880"
     CDS             16371..17000
                     /locus_tag="PWU31_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084397.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESVQGYAKKTTY"
     gene            complement(17123..18232)
                     /gene="ugpC"
                     /locus_tag="PWU31_001881"
     CDS             complement(17123..18232)
                     /gene="ugpC"
                     /locus_tag="PWU31_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL1084398.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18441..18863
                     /gene="mgsA"
                     /locus_tag="PWU31_001882"
     CDS             18441..18863
                     /gene="mgsA"
                     /locus_tag="PWU31_001882"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL1084399.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19186..19722
                     /locus_tag="PWU31_001883"
     CDS             19186..19722
                     /locus_tag="PWU31_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL1084400.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19762..21525)
                     /locus_tag="PWU31_001884"
     CDS             complement(19762..21525)
                     /locus_tag="PWU31_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084401.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21528..23243)
                     /locus_tag="PWU31_001885"
     CDS             complement(21528..23243)
                     /locus_tag="PWU31_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084402.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7544 a         4298 c         4775 g         6819 t
ORIGIN      
        1 tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt
       61 tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac
      121 aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt
      181 gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc
      241 attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc
      301 aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg
      361 agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg
      421 gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga
      481 aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt
      541 tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct
      601 tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta
      661 tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg
      721 accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac
      781 agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt
      841 attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc
      901 aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca
      961 acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat
     1021 cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt
     1081 gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg
     1141 aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga
     1201 attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa
     1261 agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc
     1321 aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt
     1381 actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac
     1441 aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc
     1501 agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat
     1561 caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct
     1621 agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa
     1681 atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg
     1741 gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa
     1801 tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc
     1861 tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat
     1921 ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga
     1981 taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg
     2041 aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg
     2101 ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta
     2161 gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa
     2221 tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc
     2281 tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct
     2341 gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat
     2401 gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac
     2461 cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag
     2521 gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa
     2581 aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac
     2641 tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc
     2701 gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg
     2761 cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat
     2821 gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt
     2881 cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc
     2941 ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag
     3001 tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg
     3061 ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt
     3121 gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca
     3181 gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag
     3241 attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa
     3301 gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc
     3361 tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt
     3421 attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt
     3481 gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt
     3541 ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca
     3601 tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg
     3661 ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca
     3721 aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg
     3781 gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg
     3841 aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa
     3901 attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt
     3961 agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg
     4021 cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg
     4081 ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta
     4141 cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga
     4201 aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag
     4261 cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat
     4321 ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca
     4381 atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag
     4441 agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt
     4501 tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag
     4561 tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg
     4621 aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga
     4681 tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa
     4741 cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa
     4801 aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg
     4861 atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt
     4921 tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata
     4981 gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa
     5041 aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac
     5101 caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg
     5161 cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac
     5221 aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa
     5281 ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc
     5341 agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa
     5401 tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc
     5461 gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat
     5521 gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat
     5581 atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa
     5641 aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct
     5701 catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa
     5761 ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa
     5821 gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca
     5881 catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg
     5941 ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa
     6001 agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc
     6061 aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa
     6121 cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa
     6181 aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc
     6241 gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat
     6301 ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca
     6361 atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca
     6421 gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg
     6481 gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca
     6541 acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag
     6601 aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt
     6661 ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa
     6721 aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat
     6781 ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg
     6841 cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc
     6901 aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga
     6961 accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact
     7021 tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc
     7081 ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga
     7141 tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa
     7201 tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg
     7261 ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga
     7321 aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt
     7381 ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa
     7441 aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct
     7501 tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc
     7561 cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat
     7621 tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt
     7681 ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat
     7741 tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt
     7801 tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat
     7861 ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg
     7921 cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg
     7981 gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca
     8041 caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga
     8101 caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca
     8161 aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag
     8221 agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac
     8281 ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac
     8341 cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc
     8401 aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga
     8461 gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg
     8521 caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg
     8581 tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat
     8641 agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt
     8701 tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt
     8761 gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt
     8821 attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag
     8881 tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa
     8941 acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta
     9001 tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac
     9061 gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct
     9121 cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa
     9181 accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga
     9241 tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa
     9301 aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta
     9361 ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga
     9421 cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga
     9481 agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga
     9541 acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa
     9601 accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa
     9661 gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc
     9721 actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca
     9781 gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc
     9841 ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg
     9901 aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg
     9961 tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt
    10021 tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt
    10081 attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc
    10141 ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg
    10201 ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct
    10261 gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga
    10321 agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc
    10381 agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg
    10441 attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac
    10501 aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc
    10561 ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga
    10621 tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga
    10681 aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa
    10741 gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat
    10801 cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa
    10861 tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc
    10921 accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat
    10981 cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt
    11041 tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa
    11101 cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta
    11161 atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc
    11221 gggcagattt taattgattc atctggtact cttttttatt gtccttacat tcataaagtt
    11281 tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat
    11341 tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata
    11401 tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa
    11461 tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga
    11521 gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca
    11581 taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag
    11641 ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa
    11701 ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg
    11761 atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc
    11821 aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt
    11881 gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca
    11941 aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg
    12001 acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt
    12061 tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc
    12121 ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca
    12181 gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac
    12241 taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg
    12301 caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat
    12361 tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat
    12421 tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa
    12481 aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg
    12541 ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt
    12601 atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga
    12661 caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg
    12721 tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg
    12781 aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt
    12841 ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa
    12901 aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga
    12961 tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag
    13021 cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga
    13081 tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt
    13141 atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga
    13201 agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga
    13261 gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt
    13321 tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat
    13381 ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt
    13441 taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga
    13501 taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa
    13561 caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat
    13621 aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag
    13681 taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa
    13741 cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa
    13801 atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac
    13861 tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa
    13921 gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa
    13981 attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc
    14041 agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca
    14101 cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg
    14161 catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt
    14221 tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat
    14281 gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt
    14341 agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt
    14401 cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt
    14461 ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga
    14521 cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc
    14581 aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt
    14641 ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc
    14701 tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct
    14761 tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc
    14821 agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt
    14881 aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg
    14941 ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga
    15001 actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga
    15061 caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag
    15121 gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag
    15181 atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag
    15241 ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg
    15301 ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg
    15361 atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga
    15421 atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg
    15481 tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag
    15541 gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag
    15601 cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga
    15661 aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat
    15721 tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt
    15781 atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg
    15841 atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga
    15901 tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg
    15961 catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc
    16021 ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt
    16081 ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt
    16141 ggtcttatta cttgatgcaa gctcttgacc actattatgc cgaacaagga gacgtgacac
    16201 aaatgttgtc gcatctccta tttttgtctc ttgatcaaaa aattcttata cttgatactc
    16261 acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag
    16321 ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa
    16381 attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa
    16441 tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat
    16501 tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat
    16561 ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt
    16621 ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac
    16681 agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt
    16741 ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat
    16801 attattctca aggatatttt tatacagtat tcatcttgat ggggattggt gggctcatcc
    16861 acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg
    16921 atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcggtt caagggtacg
    16981 cgaagaaaac gacatattag acatgatctc tcaaaaaaaa actctcatct agtaatggat
    17041 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
    17101 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
    17161 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
    17221 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
    17281 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
    17341 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
    17401 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
    17461 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
    17521 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
    17581 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
    17641 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
    17701 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
    17761 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
    17821 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
    17881 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
    17941 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
    18001 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
    18061 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
    18121 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
    18181 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
    18241 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
    18301 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
    18361 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
    18421 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
    18481 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
    18541 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
    18601 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
    18661 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
    18721 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
    18781 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
    18841 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
    18901 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
    18961 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
    19021 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
    19081 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
    19141 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
    19201 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
    19261 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
    19321 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
    19381 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
    19441 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
    19501 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
    19561 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
    19621 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
    19681 tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
    19741 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
    19801 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
    19861 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
    19921 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
    19981 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
    20041 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
    20101 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
    20161 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
    20221 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
    20281 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
    20341 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
    20401 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
    20461 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
    20521 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
    20581 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
    20641 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
    20701 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
    20761 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
    20821 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
    20881 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
    20941 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
    21001 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
    21061 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
    21121 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
    21181 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
    21241 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
    21301 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
    21361 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
    21421 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
    21481 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
    21541 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
    21601 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
    21661 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
    21721 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
    21781 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
    21841 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
    21901 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
    21961 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
    22021 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
    22081 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
    22141 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
    22201 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
    22261 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
    22321 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
    22381 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
    22441 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
    22501 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
    22561 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
    22621 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
    22681 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
    22741 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
    22801 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
    22861 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
    22921 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
    22981 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
    23041 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
    23101 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
    23161 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
    23221 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
    23281 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
    23341 cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat tttttttaaa
    23401 aaaaagaaaa tgagaaaaca tgccagtctc ttcatt
//
LOCUS       DANJMA010000041        22732 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000041 DANJMA010000000
VERSION     DANJMA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            63..209
                     /locus_tag="PWU31_001886"
     CDS             63..209
                     /locus_tag="PWU31_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL1084403.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            285..1532
                     /locus_tag="PWU31_001887"
     CDS             285..1532
                     /locus_tag="PWU31_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1084404.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1547..2299
                     /locus_tag="PWU31_001888"
     CDS             1547..2299
                     /locus_tag="PWU31_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084405.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3002..3217)
                     /locus_tag="PWU31_001889"
     CDS             complement(3002..3217)
                     /locus_tag="PWU31_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL1084406.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3236..3472)
                     /locus_tag="PWU31_001890"
     CDS             complement(3236..3472)
                     /locus_tag="PWU31_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL1084407.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3730..5883
                     /locus_tag="PWU31_001891"
     CDS             3730..5883
                     /locus_tag="PWU31_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL1084408.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5852..7267
                     /locus_tag="PWU31_001892"
     CDS             5852..7267
                     /locus_tag="PWU31_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL1084409.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7300..8334
                     /gene="sppA"
                     /locus_tag="PWU31_001893"
     CDS             7300..8334
                     /gene="sppA"
                     /locus_tag="PWU31_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL1084410.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8336..8857
                     /locus_tag="PWU31_001894"
     CDS             8336..8857
                     /locus_tag="PWU31_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1084411.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8854..9240
                     /locus_tag="PWU31_001895"
     CDS             8854..9240
                     /locus_tag="PWU31_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL1084412.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9736..9808)
                     /locus_tag="PWU31_001896"
     tRNA            complement(9736..9808)
                     /locus_tag="PWU31_001896"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9773..9775),aa:Lys,seq:ctt)
     gene            10189..10758
                     /locus_tag="PWU31_001897"
     CDS             10189..10758
                     /locus_tag="PWU31_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL1084413.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10745..11605
                     /locus_tag="PWU31_001898"
     CDS             10745..11605
                     /locus_tag="PWU31_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1084414.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11795..12499
                     /locus_tag="PWU31_001899"
     CDS             11795..12499
                     /locus_tag="PWU31_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084415.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12504..14339
                     /gene="walK"
                     /locus_tag="PWU31_001900"
     CDS             12504..14339
                     /gene="walK"
                     /locus_tag="PWU31_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL1084416.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14336..15652
                     /locus_tag="PWU31_001901"
     CDS             14336..15652
                     /locus_tag="PWU31_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084417.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15653..16522
                     /locus_tag="PWU31_001902"
     CDS             15653..16522
                     /locus_tag="PWU31_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL1084418.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16577..17386
                     /locus_tag="PWU31_001903"
     CDS             16577..17386
                     /locus_tag="PWU31_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1084419.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17489..18118)
                     /locus_tag="PWU31_001904"
     CDS             complement(17489..18118)
                     /locus_tag="PWU31_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL1084420.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18288..19580
                     /locus_tag="PWU31_001905"
     CDS             18288..19580
                     /locus_tag="PWU31_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084421.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19656..20318)
                     /locus_tag="PWU31_001906"
     CDS             complement(19656..20318)
                     /locus_tag="PWU31_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1084422.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20543..20827
                     /gene="groES"
                     /locus_tag="PWU31_001907"
     CDS             20543..20827
                     /gene="groES"
                     /locus_tag="PWU31_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL1084423.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20878..22503
                     /gene="groL"
                     /locus_tag="PWU31_001908"
     CDS             20878..22503
                     /gene="groL"
                     /locus_tag="PWU31_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL1084424.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7783 a         3609 c         4524 g         6816 t
ORIGIN      
        1 agaaaaatag gtatattgaa tcagtgacat aaaagtattt atagtttgta gggggaatcg
       61 taatggaaga aaaaaataga ctaaatgcta aacaatgttc agatcaagag cttaaaaaaa
      121 ttaaaggagg tgctggaaca aaacctcaag gaaaaccggc atcaaattta gtggaatgtg
      181 ttttttcatt atttaaaaag tgtaactaaa atttttttta tgatatccta aaatcaggag
      241 gggatctctt gattactatc gatacaaaag cttatctatt aagaatgttc gtgggaaact
      301 ttttcttttt tctcttattt tatatgacta atactgatct taacaaaaga aaatatcttt
      361 ttgtatgttt attgatacca ctcacttttt ttgttgaatt atttactgat gtttccgcag
      421 ctatacctat aatcggaagt tattttatat taaaggaaaa gaagaaaact aatatagtgc
      481 ttttaaataa attactatta tgtatgctta ttgatgattt cagatctatt attacttcca
      541 tggttatgac gtatatgttt tcatttgaag ggataaaagg gtatggttat gtagtattcc
      601 agataatgct tgattctttg tttttgctga tgtttatttg gttatataga aaattagcta
      661 taaataggat tgttgaacag tccagttcta aacttactgc tatattcatg atttatttac
      721 ttacaattgg tttatttgtt tcttatgttg ctcatcaata tcaagtattt gatcattttg
      781 tgtttggtgt cttaatattt ttaatcattc aaatgtttgt tgttctattt ttatttatta
      841 gaataactat taggcaacga gaacaatatg aacagcaatt gaaaagaaaa gaattggttt
      901 atctaaaaaa atatactgat tcactggaaa aagatcaaga aaaatttgcc agatttagac
      961 atgactataa aaatttattg ctgagtttaa aagaaatcgc taatcaaaac catgattcga
     1021 tattggaaaa agaaataaaa gaattagaga tctattctaa ttcttatcta accaataagt
     1081 ttgaatatcg ttatttaaaa aatataaaaa atgaatattt gaaaagtttg ttgatagcga
     1141 agctatacca agcaaataac aataagattt gttgtaaatt cgaatgtcca agtactatta
     1201 ttgaagttcc tgttccaata tttgactgta ttagggtgct tggaattatt ctcgataatg
     1261 caattgaagc atctatagag agtcaagata gagaactgtc tctagctatt taccgagatg
     1321 ataaacagat agaaatttcg atatcgaata gttgtagaga gatactactc tctatagata
     1381 ctctaatgaa aaaaggaata acaacaaaaa agggacatca agggttaggt ttatctagta
     1441 tagaggaaat caataaaaag aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc
     1501 aatttactac tcaagttatt ttgttgtttt gagggaagga ggatttatga gttatccaat
     1561 aatattatgt gaagatcaaa ttatacaatt aaatcagtta gaacgaatta ttgataattt
     1621 cattcttttt cacgataaag tatttaagat agtattgaaa acacaaagtc cgttagaagt
     1681 taaaaaatac ctcaaacaat tccgaccaaa gcaaggaata tatttcttag atattgattt
     1741 aaatcatgaa gttaacggta tagaattagc agaagtaatc agaaaatatg atgttcaagc
     1801 aaaaatcatt tttacaacta ctcatgatga gatgttaccc gtaacaataa aaagaagagt
     1861 tgaaacgtta ggatttgtaa caaaagatca aacactagat gagtatcgaa acgagattgt
     1921 tgagttattg ttattagcgc aagaaaggat agatgcaaca aaacaggtta cgaatcaagc
     1981 atttgttttt tccataggtt ctcaaacatt tacttttgat ataaatgaca tttattttgt
     2041 agaagcttca aaattaccac atagattatc attgtgtact aaagatgggc agtacgaatt
     2101 ttatggaaga attagtgagc ttgaaaagaa atatcctatg ttaacaagaa ttagtcgagc
     2161 gtgtttagta aatatcttta acgttaaaga aatagatttt aaaaaacgtt cactatattt
     2221 tgattcagag ttggccagaa actttacttt agggaaagca caaaaaatta aggagaagtt
     2281 gaaagaaagc actgtttagt ggttttttct ttagggagat tgcatttttc taataaaata
     2341 gattccttca ctagtgcaaa tttttctgaa tcctattaaa gttgtaccaa tttttatagc
     2401 tgttattagt tacgtgcata acagcaatat tagttactgg aggaatattt ctatggtctg
     2461 gaaccaaaaa agggccaaat gaatagttct tttcttgttt ttattaagta ccataaaggg
     2521 gaattaaaat taaatgtatg gtggaagatg cgaaagattt ggcaaattct actaattttt
     2581 agaaagtgtt tttcattttt tagttaaaaa atttgtcctg tcaagtgcaa tcctatgatt
     2641 gtacttgata ggatttgaga taattacttt cctgttttta taaatttaac ttgcaggaac
     2701 aggctttaat gcacatccga caagtacatt accaatacct cctgtaacgg ccgaaatagg
     2761 tcctcctgca gcaccaacta cagcgctgca agtccttttg taaaaatatc tctaccatta
     2821 catttccaaa gaatttttat ctgactttaa taaaagataa aaatttaata taattataaa
     2881 aactagcaca agaagcttta gtaaaaagtt ctgtccattc caaacttttg aaaagataaa
     2941 gaacatacct atgttcgtaa tcgacacaaa ccaatcttga ggtttgatat tttttaattg
     3001 tttacgcacc ggtccacatg ctgaatccag cacctaataa agctccacct acaccacaag
     3061 cagctattgc ataaggtcct ccggctgaac aaagttttac tcctgctgtt gccccagaaa
     3121 aagtagtgcc taaaactccc acggcactcc caccttgaat atttttcaac tcttctgaag
     3181 ataatataaa tgttgttttt gtgtttttaa tatgcatact acttattctc cttttttatt
     3241 tttttcgagc atcccagcca gctgcagcac ctccagcgac agctccatat aaccctccaa
     3301 caacaccgca agctggtccg caaattgctg ttcctaaacc tgcaccacct actgcccctg
     3361 tggtaagtcc acctacaact tgccccaatt ttccccaagc attattgcct ccaccaacag
     3421 tttgtgcatt tccacaatac ttagttcctt tacattttgc atgtttaaac acgcttcttt
     3481 aaataatatt ttttacggtg ctactgtaac atacgtattt gattaacagt aaaaatcttc
     3541 ctgaaactta tcaagtttat tcttgaatag taaaatttcc aaaataaatt tgattcagga
     3601 atgaaaaagt tagtgttcaa gaactgtttt caaagttttt gtggaattat gagacaatat
     3661 tttcactgta ttaaagctat attgagaatt tttctataaa aattttttag aaggagttgt
     3721 gttagataag tgataaaatc atttcctttt gtgcagcaac aagatgaaaa agattgtggt
     3781 gttgcttgcc tttcgatgat tttaaaatat tttaaaactg atatcccgat tcataaatta
     3841 cgggacctat ctgggactga tttagagggt acttcagcat ttggattgaa aaagacattt
     3901 gaaaaattgt cttttgattg tatggctatt caagcagata gtgagatctg gaaagaaaga
     3961 gatcttatct taccattaat tgcacatata ttggttgata acagctatat gcactacgtt
     4021 gtaatttata aaacgaaggg aaacgtacta tggattgcag atcccgctaa tggaaaaaat
     4081 agaaaaacga ttgaagcgtt tcaaaaagaa tggacaggta ttcttttatt acctatgcct
     4141 agagaaactt atattcctat taaagaaaaa gtagctggat taagttcttt tttgcctata
     4201 atttggaaac aaaaaggatt agtattccac atcgttttag catcgatttt cattacgttt
     4261 tttggcatag gaagctcata ttattttcaa ggtattcttg attttttcat ccctaatcaa
     4321 gcacgttcaa ctttaaatat aatttccatt ggcttgatcg ctgtctattt atttcgggta
     4381 ttgtttgaat atagtcgtag ttatcttctt attattttag gacagcgaat gagtatggca
     4441 attatgcttc agtatttcaa acatgtgtta actttaccaa tgaatttttt tgctacgaga
     4501 aaatcagggg aaattatttc tcgttttctt gatgccagta agattattga tgctttagca
     4561 agtgctacgt tatcaatatt tttagatatt ggtatggtgt tgcttgttgg ggttacgtta
     4621 gctattcaga atagtacgtt gtttttaatc actctatgct ctttgccttt ttatgtagtt
     4681 gcaatattcg ctttcgtgaa aagctatgaa aaatcgaaca gggaagaaat ggcagccggt
     4741 gcagctgtaa attctagtat catagaaagt cttaaaggaa tagaaacaat taaagcatat
     4801 agcggtgaag agaaagtata taaccgtata gaccatgaat ttgttaaatt aatgaaaaaa
     4861 tcatttcgta cggttacgct tgataatatt caacaaggaa ttaaacattt gattcaatta
     4921 attagcagtg cattaatttt atggctaggt tcttattttg ttatggataa tagaattagt
     4981 ctaggtcaac ttatcacata caatgcttta ctagtatttt tgacagatcc tttacaaaac
     5041 attattaatt tacaagtgaa aatgcaaacg gctcaagttg caaataatcg tttaaatgaa
     5101 attttcgcga ttgattcaga aaaatcgaac cagtcttttg aatatatgct ttctgaacaa
     5161 ttatttcaac aagatatcac tgtaaaaaac gtttcattct cgtatagcat gaaggcccca
     5221 acgttgaacg aaatttcatg tacaattcct tcgcacagta aaacggctat agttggagtt
     5281 agtggttctg gaaaatcaac tcttgcaaag ttatttgttc gtttttatga agcatcagaa
     5341 ggatcgatcc attatggaaa cattaatcac ttagacattc cttttcagat attacgcgat
     5401 cgagtgacat atttgcctca agagtcattt ttctttagtg gaacaatcat tgagaatctt
     5461 atattcggtt tagatagccc accaagtttc gaacaaataa tagaagtttg tgagatagtc
     5521 caactaaatg aatttattaa tcaacagcca ttacgttacg agacattgtt ggaagaaggt
     5581 ggaaataatc tttcaggagg gcaacgacaa cgattagcct tagcacgggc tttattaaaa
     5641 aatccggaca tattaatttt agatgaagca actagtggat tagatacctt gttagaacat
     5701 gcaattatga attatctcct caatttaaaa gaaaaaacga ttatttttat tgcgcatcat
     5761 ttaccaatag caaaagcatg tgatcaaatt ttagttctcc atgaagggca tttggtagaa
     5821 aaaggaacgc acgatgaact tcgttttgcc gatggtatct atcaaaaatt atgggaaatc
     5881 taggagaggg aaacaattta tgcagaataa taattggttc gaacattcta gtatctatac
     5941 taaacaacat catagcttct accgttgggt gttataccct gtaattcttt ttttatgtat
     6001 tattggattg tttttaacct ttgctaaaaa agaggttgtt attcggacaa cggctcaatt
     6061 gaaccccgaa aaaatagaga agttacaagt gccattagaa gctaaaataa tggaaaactt
     6121 cctaaaagaa aatcaatttg ttcataagga cgatgtgttg gttagattgg attgttcttt
     6181 aatagagaat gaaaaggctc agatagaaca agaaaaccaa cgtattactc aacaaataaa
     6241 gatggctcag ctatttattg aaagtataag taaaggaaaa aatttgtttt caacggatga
     6301 cagttttggc tacagtaatc aattaaagag catgttgtca gaaaaagaat cactccgcta
     6361 cgctttgaag caaagtgaat taaatgatca aaagcaatta gaagtatacg aaaagacaaa
     6421 aagacaacta gaaaaacaaa ttgagagttc agatagtaaa ttacaagaat ggcaacaagt
     6481 acaggtagct tggagtaata atcaatcatt aaaagatttt tcaaaagaaa tgatggcaaa
     6541 ctatgagaat tggcaagaac aactaaataa tgtttctgat gatcaaaaaa atcaagtgaa
     6601 actgacaatt tcagcaagca taaatgaaca aattgagcaa ctaaaaaaag aagtagaaca
     6661 gtatcagtca gaaaaagcta aattagttaa accaactact tctgagaatg acagaattag
     6721 tcaaacggaa aaaggaaagc aagagctaga acaagcaata acaactgtaa aacaaacagt
     6781 tacagagcta aatgaaaaac aagagaagaa ccagtcagtt atcaaaagtt tggacgatca
     6841 actttcaaaa ggaatactaa aagctcctgt atctggaacg gttcatttaa atgaagaaac
     6901 aaagggacaa gcagaactag ccaaaggaac agtgcttgca gaaatttatc cgatccacga
     6961 aaaatcaaaa atgaaattta cagcactttt acctgcgaat gaaagtacct atattaaaga
     7021 agggatgaag gttcatttta aattggatca aaaaggtggg gctccgatta cgatcgacgg
     7081 aactttagat gaaatttcag aaaattctac tgccactgag aaaggtgttt tttatgcagt
     7141 gaaaggaacg ttaaaaacaa ctcaatcgtt tccgtttcgt tatggattaa ctggagagtt
     7201 gtctttaatt gtaggagaaa aaacatattg gcaacaaatt gtcgatgtgt ttctaaataa
     7261 aaaataatta tttttactaa gaatatagga ggagtttata tgaataaaag aagatggatt
     7321 gctgtgggag tagcgtgcgg attattgtta ctatccatta tcgtcgcgct aattccagga
     7381 aaagataaag aggaagcatc aaatacaacc ctaacaggaa taaataagat tttttacgga
     7441 tctaacgaaa tcacagaaga aacgttagaa gaaggcgcat caaataaaaa gattgtcaaa
     7501 ctatccgtca atggagttat tgcagataca ggtgaaagca atctttttag tagggaacaa
     7561 tataaccatc agaatttttt aacacagcta aagaaaattc aagaagacaa agcggttaag
     7621 ggagtgttat tagaagtaaa ttcacctggg ggaggaatat atgaaagtgc tgagattgct
     7681 aaagaaatgg caaaaattaa aaagctagat attcctatct atactgcatt taaaaataca
     7741 gcggcaagtg gagggtacta catttctgct ggttcagata aaatatttgc tacggaagaa
     7801 acaacaactg gatcaattgg tgtcattatt tctggtttaa attattctgg cttattagaa
     7861 aaacttggcg taactgatgc gacgtataaa agtggtgcat taaaagatat gatgtcacct
     7921 caacacaaac cttcagagga agagaataaa gttatacagg aatttgtgat gagtgcttat
     7981 gatcgttttg tcaatgttgt agcaaaagga agaaatatgg atactaatgc agtgaaagaa
     8041 cttgcggatg gaagaattta tgatggtaac caagcagtag aaaatgggtt ggtggatcaa
     8101 attggttact ctgaagatgc attagattcc ctaaaaaaag agaagaaatt aactgatgcg
     8161 acggttatag aatataaaaa tgacacaact ggctttgctt cttcttggtt aggtaataag
     8221 attgctgaat ggcaagggct aaaggcaact acatcagatc gtgtaatttc agtttttgaa
     8281 aaacttggat tttctgatac tccaaaacca atgtattact atggagggct ataaaatgaa
     8341 taagaagagt ggcattgaaa ctttgcctaa ttatgtattt gcgggatttt ggttacgatt
     8401 ttttgctttt cttattgatt taatttgtat tagcatcatc acccatgcta ctataggtct
     8461 cttttacaaa atcatgggac tggaatctga caaatccatt attacttttt atggtatagc
     8521 gtcattagct atttatttaa cctattttgt acttttgact aagctgaatt caggacaaac
     8581 aattgggaaa atgatttttg ggataagagt agtgagcttt gaggaaaaag agctaagttg
     8641 gcaaactatc tttattcgtg agtgtatatg taggtttata ttaaaaaatt ttgtatttca
     8701 aattgggtat ttggtggctg ctttcacgcc caagaaacaa catattgggg attatttttc
     8761 taacacgtca gtggttgtta ttcgttcatt agaagctatt aactatggtc aaaaaatttc
     8821 taattctaaa acacttaatc tggagagaat taaatgaata agagaaaaac agagtttatt
     8881 ttgattttaa ttagtggaat aacagggatt ttatgtagtc ttttagcatt gatgggtggt
     8941 atgtttctat cttctattga ttcaactact ttaaatacta cagaactaac cagtaaggat
     9001 atagatatgt tagtcaatct aagcgggaat gttgttttat ttggaggaat taccctagtt
     9061 ttttcgataa tgattttcat aataggtttt ttagtaagaa aaaatcaaaa aacagtggtc
     9121 tatggtatta gtttactcat tttatctatt attccatttt ttcttttaac attattgtgg
     9181 gtaattcctg gagttttagg tattattgcg ggattgatgc ttctaataag aaaatcttaa
     9241 ttaggcaatg tgtagacaac gtgaaacgat taatcaatta aaagctaaat acgttttgag
     9301 aaatgataac ttgtagtcga tggaagacat gtagataaaa aaactacgtg tcttttattt
     9361 attctaattt tttcgaaaaa aagacactct ctgattgttg tattaatgga aaaggggaaa
     9421 gcagcaagta aatttagttt ttctattaaa tattcttcac tttttaacct tttatagtac
     9481 ttagatttaa tcctatatgt ttagagagtt cattggcagc atgattttgc aaagcttctc
     9541 gaattattgt tatattagaa actttactgt tcataattat gcttcttttt taactcaaag
     9601 tccaaagggc tacagtggac tttgagttaa aaataaaaag ccctagttat tttgaaaaat
     9661 aaaaaaagaa cagcgtaaat gttttcgaaa tgctttcgca ccaacattta cctgttccgt
     9721 gggtttggca cccaatgggc cgtgaggggc tcgaacccgc gacccgctga ttaagagtca
     9781 gctgctctac caactgagct aacggcccgt acaaaaagta cttaataata gtagcactaa
     9841 gaattttaaa atgcaagact acatttaaag aaaatcagaa aaatctttac ttccttatag
     9901 gaaatcattc atagattatt cacatttgct cgctattata taagcatacc aacaaacaaa
     9961 gttcttgcaa gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct
    10021 ccccggcggg tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt
    10081 tgttttcatg gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta
    10141 tcataagtct gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg
    10201 cgtcagcaat taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa
    10261 gaactccctg acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt
    10321 tcacctgtga tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac
    10381 gttaaactag gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca
    10441 tttttacttg caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg
    10501 atattgttcc aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa
    10561 caagaagcag ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa
    10621 ttcgaccagg caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca
    10681 atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa
    10741 acaaatgaaa aacaataaaa tcgcaatttt agtagattca ggtacggatg ttccagaaga
    10801 attgataaaa aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt
    10861 atatacagat aaaattgaca ttacccctga agagatctat caacgtttgc cagaggaaat
    10921 ccctggtaca tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa
    10981 agacggttat gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa
    11041 tgtggttcgt ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa
    11101 aaatattggt attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg
    11161 agtagaatgg gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt
    11221 ctttaatgtt gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc
    11281 tattgtagga accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta
    11341 ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt
    11401 caaagaaaaa atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa
    11461 agaagaagca attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat
    11521 ctactttggc cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt
    11581 cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg
    11641 ttttcctttt ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta
    11701 attttctctt ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga
    11761 aggtatgaca ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga
    11821 tgagaagccg atttcggata tcgtcaaatt caatttagcc aaagaaggct atgacgttta
    11881 tactgcatat gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat
    11941 tttacttgat ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa
    12001 aacttatgat atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct
    12061 aggcttagag ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt
    12121 tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc
    12181 agcacctgcg gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac
    12241 gaaacgagga gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc
    12301 gaagcacctt ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga
    12361 ttattttgga gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga
    12421 agataatcca agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg
    12481 taacccagaa caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt
    12541 ttaagatcgc tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct
    12601 actttatacg tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa
    12661 cggtggaatc attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg
    12721 atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca
    12781 tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac
    12841 aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt
    12901 atgttgcttt agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc
    12961 cgaccggcga tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt
    13021 cggaaatcaa taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt
    13081 caatgatcgt atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag
    13141 aacaagccat tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag
    13201 atgaacttgg acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc
    13261 aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg
    13321 gcgtcattgc gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct
    13381 tgctagatat caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg
    13441 aagaagaata tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt
    13501 caaccagtac attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc
    13561 gcgaatcagg ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa
    13621 aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt
    13681 tgacaagtat gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag
    13741 tagcaccaaa ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca
    13801 atgacctctt aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg
    13861 tcaatttcaa cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc
    13921 aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag
    13981 atacagataa aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac
    14041 cagatggggg aacgattact tgtcgcttat tagaaacgca taataacgtg attttaagta
    14101 tcaccgatca aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct
    14161 atcgagtaga taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt
    14221 ctagagaagt gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag
    14281 ggtcgacttt ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag
    14341 aatctctgaa aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt
    14401 ctcttatgca atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa
    14461 cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca
    14521 tgttacatgg caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg
    14581 attgcaattg atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga
    14641 caaatcacag tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc
    14701 gccttttttg ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt
    14761 ggcaaaagaa agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa
    14821 aatccgattt ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg
    14881 gacagatatc aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga
    14941 accatcgacg atcgacacgc aatatgctac gaaacattcg atcaaactga agaagtatag
    15001 ttatatcctg tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga
    15061 tagcgtgaaa aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat
    15121 gtctatccat tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga
    15181 aaatgctagt gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg
    15241 gaacctcaga tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg
    15301 cttcccggtt tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac
    15361 gaatggttct gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc
    15421 ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa
    15481 gggagcagat agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa
    15541 agaaacgaat cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg
    15601 gtattcagct aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt
    15661 caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag
    15721 cagttatcat gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc
    15781 aatccaacaa cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa
    15841 caagcaaggc tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta
    15901 tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt
    15961 gacgaaatct ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag
    16021 tagttccttg gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt
    16081 gtacacgaaa aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga
    16141 gtatgaaggc atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa
    16201 cgataatgat tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct
    16261 gcgcgaaaag acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa
    16321 caaaatacca agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa
    16381 agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa
    16441 agtggtccaa attgagagcg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt
    16501 accaaaagtg gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa
    16561 gggaagtagg agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag
    16621 tggaagttcg ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc
    16681 aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca
    16741 attagatgga atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt
    16801 ggctcgtaag tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc
    16861 actgattgga aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac
    16921 attcggcgat atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt
    16981 ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag
    17041 tgattatatg cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga
    17101 tcttgagatg ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga
    17161 tagaggacat ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg
    17221 gaccaaacga atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca
    17281 tttaacaatg gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat
    17341 ctatgataca gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat
    17401 aaaaatataa aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt
    17461 tttttagcat attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt
    17521 catgtttctc atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg
    17581 tactgtcgct agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac
    17641 gtttgccatg tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag
    17701 attgtttcaa ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat
    17761 ttttaggtac ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg
    17821 cacccccaac gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca
    17881 aagaagtttg atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat
    17941 caaaagatgc aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat
    18001 ctgatggcaa tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg
    18061 cttcgtctaa aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa
    18121 aaaacctcct tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc
    18181 cgatttttga aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta
    18241 aattaagaaa actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt
    18301 tagatcgtgc ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt
    18361 tgcgtaatgg aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat
    18421 ttgctcattt tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt
    18481 attttacaca agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca
    18541 ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct
    18601 tgtctgctta tctactgctt attcagccac taaatactca gatagatact gtctggacag
    18661 gtgctgaagg gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac
    18721 gtttagtaag acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc
    18781 agtcttttac ttcattgatt ccaatcattg ttatagtaac ttttacgctt atcggttcag
    18841 cggtttttca acatttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc
    18901 ttcccattca atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc
    18961 cgtttttatg gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg
    19021 tcttgcaagc gaattcatta gcaaatgcag aaatcttagc ctcaggacaa gagttgacgg
    19081 tggcaaatgg cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg
    19141 gtgctggatt gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat
    19201 acagagaatt aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta
    19261 ttttttccac accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg
    19321 tcgtttcagg attgattacc tattccgctt tatattttgg attcgttcct ttatttacag
    19381 cagtccaagt cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc
    19441 ctgcagcgat tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt
    19501 tgaaaaaaat agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag
    19561 cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca
    19621 ccaatctagg atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct
    19681 aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc
    19741 cgaccagttg ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga
    19801 tgaccatcat tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa
    19861 tttgaatatc gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg
    19921 ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata
    19981 ttttgtgtat tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg
    20041 atgctttgca gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga
    20101 gcagaagctt tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa
    20161 atagctagga caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac
    20221 gtagatttta caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag
    20281 caaaaaatgc tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat
    20341 aacaagcatg caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag
    20401 cagctgtcag aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat
    20461 gagtattatt aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa
    20521 agagttatcg gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt
    20581 cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa
    20641 accccaaact gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa
    20701 agttcctgcc gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga
    20761 agtaaaatac gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt
    20821 tgagtaaatt tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg
    20881 gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa
    20941 ttagcagata cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa
    21001 tcttacggct ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta
    21061 gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat
    21121 gatattgcag gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa
    21181 ggtttaaaaa acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg
    21241 gcaacaaaag cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa
    21301 gccattgcac aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct
    21361 gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc
    21421 gaaacagaac tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat
    21481 atggtaacag ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca
    21541 gacaaaaaaa tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa
    21601 tcacgtccat tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta
    21661 ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat
    21721 cgtcgtaaag caatgcttga agatatcgct attttgacag gtggtacagt aattactgac
    21781 gatctaggtt tagaattgaa agatgcaaca atcgaaaact taggaaatgc ttctaaagtt
    21841 gttgtagata aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa
    21901 gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa
    21961 aaattacaag aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca
    22021 gctactgaaa cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca
    22081 cgtgccgcag tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc
    22141 agcaaagttt ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt
    22201 cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg
    22261 attgttgata aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa
    22321 tgggtaaaca tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg
    22381 caaaatgccg cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa
    22441 ccagaaccag cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg
    22501 taagaaaaag caacttgctt atctacaact gatcattagt gaaagacaga taaaaagagg
    22561 ctggaacaga agcgtttaac tccaagaaat aagaagaaat tcacggaaat tgcttttcaa
    22621 atttttgtga atttcagctt atttccgaag gagttgcttc tgcttccgct gtttatacgt
    22681 gtttagagcg tgagacaaaa gtgttcttta cttttgtctc acgctctttt tt
//
LOCUS       DANJMA010000042        22301 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000042 DANJMA010000000
VERSION     DANJMA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            5..403
                     /locus_tag="PWU31_001909"
     CDS             5..403
                     /locus_tag="PWU31_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1084425.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="PWU31_001910"
     CDS             387..1082
                     /locus_tag="PWU31_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL1084426.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="PWU31_001911"
     CDS             complement(1676..2563)
                     /locus_tag="PWU31_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1084427.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="PWU31_001912"
     CDS             2748..3587
                     /locus_tag="PWU31_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1084428.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="PWU31_001913"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="PWU31_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL1084429.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="PWU31_001914"
     CDS             5055..5411
                     /locus_tag="PWU31_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084430.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="PWU31_001915"
     CDS             5414..6571
                     /locus_tag="PWU31_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084431.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="PWU31_001916"
     CDS             complement(6597..7037)
                     /locus_tag="PWU31_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL1084432.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="PWU31_001917"
     CDS             7197..7703
                     /locus_tag="PWU31_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1084433.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="PWU31_001918"
     CDS             7871..9784
                     /locus_tag="PWU31_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL1084434.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="PWU31_001919"
     CDS             9789..10790
                     /locus_tag="PWU31_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084435.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="PWU31_001920"
     CDS             complement(10992..11198)
                     /locus_tag="PWU31_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL1084436.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="PWU31_001921"
     CDS             complement(11844..12692)
                     /locus_tag="PWU31_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1084437.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12691..13758
                     /gene="gnd"
                     /locus_tag="PWU31_001922"
     CDS             12691..13758
                     /gene="gnd"
                     /locus_tag="PWU31_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL1084438.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="PWU31_001923"
     CDS             13760..14761
                     /locus_tag="PWU31_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL1084439.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="PWU31_001924"
     CDS             14758..15042
                     /locus_tag="PWU31_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL1084440.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="PWU31_001925"
     CDS             15075..15872
                     /locus_tag="PWU31_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1084441.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="PWU31_001926"
     CDS             15886..16719
                     /locus_tag="PWU31_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084442.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="PWU31_001927"
     CDS             16737..17594
                     /locus_tag="PWU31_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1084443.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="PWU31_001928"
     CDS             17616..18125
                     /locus_tag="PWU31_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084444.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="PWU31_001929"
     CDS             18162..18563
                     /locus_tag="PWU31_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084445.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="PWU31_001930"
     CDS             18675..19028
                     /locus_tag="PWU31_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084446.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="PWU31_001931"
     CDS             19062..19505
                     /locus_tag="PWU31_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1084447.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="PWU31_001932"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="PWU31_001932"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1084448.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="PWU31_001933"
     CDS             20600..21313
                     /locus_tag="PWU31_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1084449.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21520..21669)
                     /locus_tag="PWU31_001934"
     CDS             complement(21520..21669)
                     /locus_tag="PWU31_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084450.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22065..22265
                     /locus_tag="PWU31_001935"
     CDS             22065..22265
                     /locus_tag="PWU31_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1084451.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7682 a         3430 c         4620 g         6569 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct ccttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaatcggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaacggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa a
//
LOCUS       DANJMA010000043        22023 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000043 DANJMA010000000
VERSION     DANJMA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(61..1371)
                     /locus_tag="PWU31_001936"
     CDS             complement(61..1371)
                     /locus_tag="PWU31_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL1084452.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="PWU31_001937"
     CDS             complement(1383..2801)
                     /locus_tag="PWU31_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1084453.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGSIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="PWU31_001938"
     CDS             complement(3041..4609)
                     /locus_tag="PWU31_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1084454.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="PWU31_001939"
     CDS             complement(4613..4894)
                     /locus_tag="PWU31_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL1084455.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="PWU31_001940"
     CDS             complement(4891..5814)
                     /locus_tag="PWU31_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL1084456.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="PWU31_001941"
     CDS             complement(5811..6689)
                     /locus_tag="PWU31_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL1084457.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="PWU31_001942"
     CDS             complement(6676..8013)
                     /locus_tag="PWU31_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL1084458.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="PWU31_001943"
     CDS             complement(8016..9119)
                     /locus_tag="PWU31_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1084459.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="PWU31_001944"
     CDS             complement(9193..10701)
                     /locus_tag="PWU31_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1084460.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="PWU31_001945"
     CDS             complement(10667..11359)
                     /locus_tag="PWU31_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1084461.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="PWU31_001946"
     CDS             complement(11381..12355)
                     /locus_tag="PWU31_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084462.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="PWU31_001947"
     CDS             12655..13527
                     /locus_tag="PWU31_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084463.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="PWU31_001948"
     CDS             complement(14109..16307)
                     /locus_tag="PWU31_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084464.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="PWU31_001949"
     CDS             16539..17531
                     /locus_tag="PWU31_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084465.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="PWU31_001950"
     CDS             17591..19012
                     /locus_tag="PWU31_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL1084466.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="PWU31_001951"
     CDS             19005..20504
                     /locus_tag="PWU31_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL1084467.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="PWU31_001952"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="PWU31_001952"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1084468.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6668 a         4492 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggatcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gac
//
LOCUS       DANJMA010000044        21882 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000044 DANJMA010000000
VERSION     DANJMA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(278..478)
                     /locus_tag="PWU31_001953"
     CDS             complement(278..478)
                     /locus_tag="PWU31_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1084469.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(783..2015)
                     /locus_tag="PWU31_001954"
     CDS             complement(783..2015)
                     /locus_tag="PWU31_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL1084470.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2270..2839
                     /locus_tag="PWU31_001955"
     CDS             2270..2839
                     /locus_tag="PWU31_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1084471.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3017..3457)
                     /locus_tag="PWU31_001956"
     CDS             complement(3017..3457)
                     /locus_tag="PWU31_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL1084472.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3615..4379
                     /gene="map"
                     /locus_tag="PWU31_001957"
     CDS             3615..4379
                     /gene="map"
                     /locus_tag="PWU31_001957"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL1084473.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4411..5334
                     /locus_tag="PWU31_001958"
     CDS             4411..5334
                     /locus_tag="PWU31_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL1084474.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5410..6549
                     /locus_tag="PWU31_001959"
     CDS             5410..6549
                     /locus_tag="PWU31_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL1084475.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6542..7342
                     /locus_tag="PWU31_001960"
     CDS             6542..7342
                     /locus_tag="PWU31_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1084476.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7342..8169
                     /locus_tag="PWU31_001961"
     CDS             7342..8169
                     /locus_tag="PWU31_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1084477.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8147..8881
                     /locus_tag="PWU31_001962"
     CDS             8147..8881
                     /locus_tag="PWU31_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1084478.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8981..9847
                     /gene="rfbA"
                     /locus_tag="PWU31_001963"
     CDS             8981..9847
                     /gene="rfbA"
                     /locus_tag="PWU31_001963"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL1084479.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9861..10433
                     /gene="rfbC"
                     /locus_tag="PWU31_001964"
     CDS             9861..10433
                     /gene="rfbC"
                     /locus_tag="PWU31_001964"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL1084480.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10455..11483
                     /gene="rfbB"
                     /locus_tag="PWU31_001965"
     CDS             10455..11483
                     /gene="rfbB"
                     /locus_tag="PWU31_001965"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL1084481.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11581..12432
                     /gene="rfbD"
                     /locus_tag="PWU31_001966"
     CDS             11581..12432
                     /gene="rfbD"
                     /locus_tag="PWU31_001966"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL1084482.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12467..14500
                     /locus_tag="PWU31_001967"
     CDS             12467..14500
                     /locus_tag="PWU31_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084483.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14544..15824
                     /locus_tag="PWU31_001968"
     CDS             14544..15824
                     /locus_tag="PWU31_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL1084484.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16034..16840
                     /locus_tag="PWU31_001969"
     CDS             16034..16840
                     /locus_tag="PWU31_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084485.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16852..18072
                     /locus_tag="PWU31_001970"
     CDS             16852..18072
                     /locus_tag="PWU31_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084486.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18062..19648
                     /locus_tag="PWU31_001971"
     CDS             18062..19648
                     /locus_tag="PWU31_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1084487.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19687..21825
                     /locus_tag="PWU31_001972"
     CDS             19687..21825
                     /locus_tag="PWU31_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1084488.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6901 a         3707 c         4547 g         6727 t
ORIGIN      
        1 cataattgtg tttttccttc catcagcata aaagttcacc tcaaaaagag tatacgaacg
       61 aatgttcttt ttgtgaaggc ggaatttaat atactgaata atagaaaaca agtacttgtg
      121 ccaaattatg tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag aaaaccttct
      181 atattaagat ttcctgcaat aacaaacaca acagaagacg tgtaatagtt agtcaggaac
      241 gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa gtagacattg
      301 tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat
      361 aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga agagaaaata
      421 gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg attttcatac
      481 ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc caacgaaaga
      541 tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca atgggaatga
      601 actggagatg actttttatt atttttagta atagctgatg ggttaatact tacaactttc
      661 cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa ggatctaagc
      721 caatagctta gatcctttat tctatgcttt atattatttt ttgcatgtgt gcgaaagtat
      781 actcaagccc aatcgccatt tcggaaaatc ggtactgtag agccatcttc acggattccg
      841 tcgatatcca tcttgtcaga tcctaccatg aaatctacgt gggtttgact tctattcaat
      901 ccagcttctg ctaattcttc atcggacatt tctgtaccgc ctttcacact gaaggcataa
      961 gcagaaccta gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg
     1021 atgccagatt gagaaatagg tgaagggtca ggtacaagtg cgacttctcc taaacgacgc
     1081 gcaccttcat ctgtatcaag cagttttgct aagacatctt ctccttgttc ggcagaaaaa
     1141 tcaacaactt ttccatcttt aaaagtaaat ttcatacccg agatgatggt gccagcataa
     1201 ctcagtggct ttgtacttga aatgtaacca tctactcgat gactgtcagg tgctgtgaac
     1261 acttcttccg tcggcatatt tgccatgaat ttttctccac gagcattata gcttccagcg
     1321 ccttcccaaa gatggttttt aggcaatccg ataatgatat ctgtaccggg agcagtatag
     1381 tgaagggcag aaaattgttc tcgattcaat tcttctgctt tttttgctaa tttttcatca
     1441 tgcttcttcc atgctaagac agggtcttct tcatagacac gagtcgtttt gaaaatctga
     1501 tcccataatg cctcaacttg tttttcttct ggcagttctg ggaagacttt tgctgcccat
     1561 tgtttaccag ccgcagccac gacagtccaa ctgactttat ttgcttgagt tgccttgcgt
     1621 aaattcatta gtgctttgcc attcgctgat tgataagacg ctactcgatg gctatctaca
     1681 ccagcaaatg catcgggatc ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc
     1741 cattcatccg cttgatcgat tttagattgt ggtacattct caatacgatc cgttgcagca
     1801 tgtaaaagaa attctctttg gatctggtcg tcagtccatt gaacgataac ctcagcggct
     1861 cctagtttat aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc
     1921 tgtaaaacaa ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat taagcgtgca
     1981 tattttttta gaagttcatt aaaatcagat aacataataa aaattcctcc tcaccatttt
     2041 ttctggtaac tattagcatg ataccattta attatgattc aaacaatgag gctttaaata
     2101 aaaaaaggag gttatttttt taaggttaag tttcatgata tatgttacta cttgctattt
     2161 cagcttgtga agaaaatcaa aaactaattt tgcatatcaa aaaaaacatg ttacaatgaa
     2221 attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa
     2281 aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg
     2341 atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat
     2401 ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga
     2461 tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc
     2521 ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga
     2581 agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt
     2641 gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg
     2701 gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac
     2761 tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga
     2821 accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat
     2881 gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga
     2941 actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc
     3001 ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt
     3061 cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc
     3121 ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag
     3181 tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt
     3241 cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta
     3301 cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga
     3361 cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc
     3421 ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt
     3481 atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac
     3541 ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa
     3601 aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat
     3661 ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca
     3721 caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg
     3781 cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt
     3841 gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt
     3901 gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta
     3961 cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc
     4021 aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag
     4081 gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg
     4141 aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca
     4201 tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta
     4261 acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg
     4321 ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc acttattgat
     4381 ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat
     4441 gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt
     4501 acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct
     4561 tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga
     4621 gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca
     4681 caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa
     4741 agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat
     4801 tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt
     4861 ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta
     4921 caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat cacattggta
     4981 gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg
     5041 aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg
     5101 tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt
     5161 tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc
     5221 atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca
     5281 agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa
     5341 gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta
     5401 ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta
     5461 tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa
     5521 ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt
     5581 atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt
     5641 gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat
     5701 gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt
     5761 gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc
     5821 tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg
     5881 attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga
     5941 atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt
     6001 cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta
     6061 ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg
     6121 aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca
     6181 gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat
     6241 aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg
     6301 acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga
     6361 agaatcggaa cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa
     6421 ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg
     6481 aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata
     6541 gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa
     6601 aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat
     6661 ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa
     6721 atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa
     6781 tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt
     6841 ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat
     6901 gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct
     6961 ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga
     7021 caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg
     7081 gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt
     7141 tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc
     7201 aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc
     7261 gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa
     7321 gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag
     7381 caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa
     7441 attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa
     7501 gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt
     7561 gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg
     7621 ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct
     7681 gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat
     7741 atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca
     7801 atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct
     7861 gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa
     7921 actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa
     7981 gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat
     8041 gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc taaacagttc
     8101 ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg
     8161 aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag
     8221 agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga
     8281 tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg
     8341 aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca
     8401 gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa
     8461 agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag tagtttctga
     8521 tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa
     8581 ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc
     8641 tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga
     8701 tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct
     8761 gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa
     8821 aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg
     8881 ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga tgagataata
     8941 acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc
     9001 tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc
     9061 gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa
     9121 agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa
     9181 cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc
     9241 acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg
     9301 ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga
     9361 tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga
     9421 atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa
     9481 ttacgctgta actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat
     9541 caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa
     9601 aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca
     9661 tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa
     9721 agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct
     9781 ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc
     9841 tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg
     9901 tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa
     9961 aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg
    10021 aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga
    10081 ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga
    10141 cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac
    10201 ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt
    10261 gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag
    10321 cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc
    10381 cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa
    10441 ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta
    10501 atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac
    10561 tgacttatgc aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg
    10621 tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag
    10681 ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg
    10741 tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac
    10801 gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc
    10861 caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa
    10921 gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt
    10981 ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg
    11041 aaaaatttat tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg
    11101 gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc
    11161 tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag
    11221 ataataagac cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg
    11281 accatgtaaa cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc
    11341 gagaagagct aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga
    11401 tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag
    11461 catatgccaa aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca
    11521 tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat
    11581 atgtttttat taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat
    11641 gaaaaaaatc tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag
    11701 aaaacaatgg cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac
    11761 acagcagtag ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat
    11821 ggcacaagaa atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt
    11881 acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg
    11941 aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt
    12001 ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc
    12061 gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag
    12121 tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa
    12181 aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc
    12241 gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt
    12301 ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta
    12361 ggttttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa
    12421 atgaataagt aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac
    12481 aagaagtgca cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct
    12541 tgacctgttt ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac
    12601 ttttaattta aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga
    12661 tgggaaaaaa actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct
    12721 ggtgcagaca cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga
    12781 ttattcatta aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac
    12841 ggttatcgga aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc
    12901 cttttattgg gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat
    12961 attaacgaag aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc
    13021 agcagtccag tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact
    13081 gatgattgct ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat
    13141 gatgcttttg gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca
    13201 agtgatgctg gcgtacctgc tgatttttta ttttgttggc ctgcttattt actatagcaa
    13261 gaaagtttca tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg
    13321 cggagtcatg gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac
    13381 cttatatcca ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc
    13441 tttccttaca aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt
    13501 ggccctgttc tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg
    13561 caaaaaagat agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc
    13621 tatcttttgg attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta
    13681 tgttcctcca ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg
    13741 gtttatcgct tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt
    13801 ggcagtaaat gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt
    13861 ttcggatttt gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt
    13921 atttggctgg agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt
    13981 tttcgtcaat ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga
    14041 aatccaaaaa atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata
    14101 taacttcacc ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat
    14161 agctgcatgg aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc
    14221 tcatacaaga gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat
    14281 gttttcggtc acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag
    14341 taaacgacca ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg
    14401 accggataaa gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc
    14461 acaaacacaa actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc
    14521 ctgaaatatt agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca
    14581 acaaaatgag tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag
    14641 caggggtagg ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt
    14701 ttagtgtggc tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt
    14761 atctaggtgt tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg
    14821 agtctttatt tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat
    14881 ggatcgagtg gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg
    14941 gaatcacgtt atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg
    15001 ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga
    15061 tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa
    15121 tcatcggtgg gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag
    15181 gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc
    15241 gtgtcattcg tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt
    15301 cttatctggc ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc
    15361 ataaattcta tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat
    15421 ttatcttcca gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga
    15481 atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc
    15541 gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg
    15601 cggtgatgat gcagtcattt atggaggata cattgacttc ataccgatgg gttccgatta
    15661 tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt acggaggaat
    15721 tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac
    15781 aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga
    15841 gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct
    15901 gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac
    15961 gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct
    16021 tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag
    16081 aaaattactg ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt
    16141 aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt
    16201 tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt
    16261 taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt
    16321 tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac
    16381 gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc
    16441 gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg
    16501 catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc
    16561 tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt
    16621 atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta
    16681 ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg
    16741 aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt
    16801 gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa
    16861 tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat
    16921 cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg
    16981 aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga
    17041 tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg
    17101 atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag
    17161 tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa
    17221 gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggcgacca tatttatcaa
    17281 cctgtaaaaa cctattcaag tgggatgttc gttcgtctag cttttgctgt agcgatcaat
    17341 gtgaatcctg atattttgat tgtggatgaa gcattggcag taggagatct tgaatttcaa
    17401 ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt
    17461 tctcatgata ttagttcgat tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga
    17521 aaagttgtcg aatatggtga tacgatggaa gtaacgacga attacgaaaa ctttttgaag
    17581 aaaaaatcag ttaaaacagt agatcgaaat aaaaaccaag tagaacgctt cgcagctcca
    17641 gatatcgtag acgtgacgaa agccgaattg ttggataaag ataaaaatcc agtggatatg
    17701 ctggaacaag gagaaaaact atacgttaaa gtaacctatg aagtaaaaga cgatacaatc
    17761 aaaaatccag ttttaggaat tgctctgcgt acagtcgaca atttttatgt gtgcggactc
    17821 aatactctac tagatggtat caagatccct tggaaaaaag gtgagaatat cttttatctg
    17881 gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa
    17941 gaaaatgcaa cggttccttt ggtatacaag acaaaataca tgaacttgtt tgtcagcggt
    18001 tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc
    18061 aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc
    18121 acagatcaaa ccaggaagta aagttttaga gtttggtcct ggaaacggcc gattaacgaa
    18181 acatttgatc ggagcaaaac aatgtgaagt gagcattgtc gagctagata aggaattgtt
    18241 cgattttgtc agcgagtttg cccaagatgg attttatgga gatatcgaaa gttttgaatg
    18301 ggcaaactat tatgctggac aaacatttga ttacgttttg tttgccgatg ttttagaaca
    18361 tttggtagat cctggaaaaa cgttgaagaa agttagagaa tttttgaatg aagaaggaga
    18421 aattctaatt acattcccta accttgcgca taattctgtc atgattgatt tgttcaataa
    18481 tcaattgcca tgggcttctt atggtttgct agatgaaacc cataattctt tttatactca
    18541 tgatggcttc caaaaagttt ttgaaaaagc aggattatac atcaatattg aagattattt
    18601 gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt
    18661 acgttatgat ttcaaaatgc gaccatttgg cgaagtctac caatacttct tctcattgat
    18721 gaaacatcca gtagcacaaa gttccattgc gcagccgcaa aattctaatt atgtgaaagt
    18781 attagaagta atccagcaaa cgaaacaaga tgaaataatt caacaaatac cattcaataa
    18841 tttcacaggg gaaaatgaga cactaagttt ccatgtatca gaattagcag aacgaatggt
    18901 gtttcgcttc gcgcagcaac ctagctttat cgaattcagc gcagaagctg ctggagaaaa
    18961 actcacattt atcgattcaa atgctgtagt caaaacagta aatgattgtt atttgtttga
    19021 tggtaaagat ttaccagagt tctttttaac ggatattgca gggaaagaag tgactatcca
    19081 ttgtcactat cgttttatcg gagaattgac accaacaatg aaagaattac tggaaacaat
    19141 ccgtccaatg gctgaagtta caaagcagct ttctgagaaa aatgatgaat tagaaagaga
    19201 aaatcattat cttattgaga ataatacaag attagatcag acgttgaaaa caaccataaa
    19261 tcgctattgc acgttgttgg aaagtgatga atggacaatc aaacatagat tagggcgacg
    19321 taagaaagaa acatcgaaaa aaatccaaga aaaagagctt tctctatgta ttgacgaaaa
    19381 aatctgggat gcagaaacaa agatattgaa aatcatcggg tggggaatcg ctaattcgga
    19441 tcgccaacca ttgtcctata agttatcagc cgaccaatca ccattttttg aagcaattcc
    19501 tgttttacga gaagaagtca atcaagcaaa acagctagct gaaggagcaa aagcaggatt
    19561 tgaactgcgg atcctttgcg agaaggaacg ttcattctta gttgaagcag tggctcaaaa
    19621 tggtcaatca tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa
    19681 cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc
    19741 actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg
    19801 ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc
    19861 aatcatttat ataatcttga tgttaagacc caaagcggtt ttaaggtacg tttatcaggg
    19921 aaaatgagag gaaaagcaat cttagacttc caaacagcaa gacatcaaaa tggcatagct
    19981 gtgaaattaa acggtaaata tccttatgat gacggcattg aatcttcatg ggaaagaaaa
    20041 aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcaatc
    20101 cgccgggtca aactggaatt gaaaccagga tcgatcgact atgcagagtg gatcagtcgt
    20161 catgagaaac ctgatctgaa agagcaaagg aaagaagtac aaggatttgc ctaccgccca
    20221 ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt
    20281 gattcggtac ttgatcaaac gtatgatcac tgggaactat gtatcagtga tgatgcttct
    20341 actgatccag ctatccgaaa gtgtctagaa tcctatcaag caaaagatga tagaattaaa
    20401 gtcgttttcc gacaagaaaa tggacacatc agtctggcaa ctaattctgc attggaaatg
    20461 gcagaaggag agtttatagc gctcttagat aatgatgacg aattacctcc atttgcctta
    20521 tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atttgatcta cagtgacgaa
    20581 gataaaatcg atgcagatgg gaatcgcttt gacccgcact tcaaagcaga ctggtctccc
    20641 gatacattga tgggaaataa ttatatctct cacttaggcg tatatcggac aagcattgtt
    20701 aaggaacttg gcggtttccg taaaggatat gaaggctctc aagattatga tttggttctt
    20761 cgtgtgacag aacaaattcc tgcagaacac atttaccaca ttgaccgtgt cttgtatcat
    20821 tggcggacga tcccgggatc aactgcaagc aatggagaag ctaaaagcta catctatgat
    20881 tctggagtga aagcattgac agatgctcta tctagaagaa atatcaaggg aagcgtacat
    20941 cctggcagaa tttctggttt ttatgaaatc acttatgatg ttttgcaaga agacttggtc
    21001 agtgtgatca ttccaactaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc
    21061 atcgaaaaaa cgtcttatcc aaattatgaa atcattattg cagataatgg aagtacagat
    21121 cctaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg atttatcgtc
    21181 gaattaattg acattccatt caattattca cgtatcaaca atctggcagc agaaaaagca
    21241 aacggcaaat actttttatt cttgaataac gatacagaag tgattgaacc tgactggatg
    21301 acagcgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaattattt
    21361 tatccggatg atacaacgca gcatgcaggt gtattattag ggatcggtgg cgttgcagga
    21421 catgcattga ataactatga tcgaacacat tgtggttact ttggccgatt ggtcattgac
    21481 gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct
    21541 gtcaatggat ttgatgaaat attagaagta gcattcaacg atgttgactt gtgcttgaaa
    21601 gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa
    21661 tctaaatcaa gaggttatga agatacacca gaaaaacaaa gacgttttgc tggggaaatc
    21721 aaaaaaatgc aagataagtg gccttcttat attgctcatg acccatttta taacgataat
    21781 ctaactaaag aaggaatcgg cgatttttcg ttgagacctg attgattaaa agaactgtat
    21841 tttcattttt attaatgaaa atacagttct tttaatttaa tt
//
LOCUS       DANJMA010000045        19855 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000045 DANJMA010000000
VERSION     DANJMA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(135..569)
                     /locus_tag="PWU31_001973"
     CDS             complement(135..569)
                     /locus_tag="PWU31_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1084489.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(801..1511)
                     /locus_tag="PWU31_001974"
     CDS             complement(801..1511)
                     /locus_tag="PWU31_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1084490.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1501..2361)
                     /locus_tag="PWU31_001975"
     CDS             complement(1501..2361)
                     /locus_tag="PWU31_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL1084491.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2366..3004)
                     /locus_tag="PWU31_001976"
     CDS             complement(2366..3004)
                     /locus_tag="PWU31_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL1084492.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3019..3318)
                     /locus_tag="PWU31_001977"
     CDS             complement(3019..3318)
                     /locus_tag="PWU31_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084493.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3361..4845)
                     /locus_tag="PWU31_001978"
     CDS             complement(3361..4845)
                     /locus_tag="PWU31_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1084494.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4865..5326)
                     /locus_tag="PWU31_001979"
     CDS             complement(4865..5326)
                     /locus_tag="PWU31_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084495.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5344..6411)
                     /gene="ulaG"
                     /locus_tag="PWU31_001980"
     CDS             complement(5344..6411)
                     /gene="ulaG"
                     /locus_tag="PWU31_001980"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL1084496.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6629..7399
                     /locus_tag="PWU31_001981"
     CDS             6629..7399
                     /locus_tag="PWU31_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1084497.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7672..8499)
                     /locus_tag="PWU31_001982"
     CDS             complement(7672..8499)
                     /locus_tag="PWU31_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084498.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9067..10302
                     /locus_tag="PWU31_001983"
     CDS             9067..10302
                     /locus_tag="PWU31_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1084499.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11301..11561)
                     /locus_tag="PWU31_001984"
     CDS             complement(11301..11561)
                     /locus_tag="PWU31_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1084500.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11588..12889)
                     /locus_tag="PWU31_001985"
     CDS             complement(11588..12889)
                     /locus_tag="PWU31_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084501.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13181..13639)
                     /locus_tag="PWU31_001986"
     CDS             complement(13181..13639)
                     /locus_tag="PWU31_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1084502.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(13652..14983)
                     /locus_tag="PWU31_001987"
     CDS             complement(13652..14983)
                     /locus_tag="PWU31_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL1084503.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(14994..16436)
                     /locus_tag="PWU31_001988"
     CDS             complement(14994..16436)
                     /locus_tag="PWU31_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1084504.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(16423..17187)
                     /locus_tag="PWU31_001989"
     CDS             complement(16423..17187)
                     /locus_tag="PWU31_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL1084505.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(17291..18166)
                     /locus_tag="PWU31_001990"
     CDS             complement(17291..18166)
                     /locus_tag="PWU31_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1084506.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(18421..18505)
                     /locus_tag="PWU31_001991"
     tRNA            complement(18421..18505)
                     /locus_tag="PWU31_001991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18469..18471),aa:Leu,seq:caa)
     gene            complement(18523..18594)
                     /locus_tag="PWU31_001992"
     tRNA            complement(18523..18594)
                     /locus_tag="PWU31_001992"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18560..18562),aa:Gln,seq:ttg)
     gene            complement(18604..18676)
                     /locus_tag="PWU31_001993"
     tRNA            complement(18604..18676)
                     /locus_tag="PWU31_001993"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18641..18643),aa:His,seq:gtg)
     gene            complement(18738..18822)
                     /locus_tag="PWU31_001994"
     tRNA            complement(18738..18822)
                     /locus_tag="PWU31_001994"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18786..18788),aa:Leu,seq:caa)
     gene            complement(18843..18913)
                     /locus_tag="PWU31_001995"
     tRNA            complement(18843..18913)
                     /locus_tag="PWU31_001995"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18879..18881),aa:Cys,seq:gca)
     gene            complement(18959..19030)
                     /locus_tag="PWU31_001996"
     tRNA            complement(18959..19030)
                     /locus_tag="PWU31_001996"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18996..18998),aa:Gln,seq:ttg)
     gene            complement(19042..19114)
                     /locus_tag="PWU31_001997"
     tRNA            complement(19042..19114)
                     /locus_tag="PWU31_001997"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19079..19081),aa:His,seq:gtg)
     gene            complement(19117..19190)
                     /locus_tag="PWU31_001998"
     tRNA            complement(19117..19190)
                     /locus_tag="PWU31_001998"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19156..19158),aa:Trp,seq:cca)
     gene            complement(19197..19277)
                     /locus_tag="PWU31_001999"
     tRNA            complement(19197..19277)
                     /locus_tag="PWU31_001999"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19241..19243),aa:Tyr,seq:gta)
     gene            complement(19279..19354)
                     /locus_tag="PWU31_002000"
     tRNA            complement(19279..19354)
                     /locus_tag="PWU31_002000"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19319..19321),aa:Phe,seq:gaa)
     gene            complement(19390..19462)
                     /locus_tag="PWU31_002001"
     tRNA            complement(19390..19462)
                     /locus_tag="PWU31_002001"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19427..19429),aa:Asp,seq:gtc)
     gene            complement(19472..19544)
                     /locus_tag="PWU31_002002"
     tRNA            complement(19472..19544)
                     /locus_tag="PWU31_002002"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19509..19511),aa:Val,seq:tac)
     gene            complement(19560..19631)
                     /locus_tag="PWU31_002003"
     tRNA            complement(19560..19631)
                     /locus_tag="PWU31_002003"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19596..19598),aa:Glu,seq:ttc)
     gene            complement(19646..19735)
                     /locus_tag="PWU31_002004"
     tRNA            complement(19646..19735)
                     /locus_tag="PWU31_002004"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19699..19701),aa:Ser,seq:gga)
     gene            complement(19775..19848)
                     /locus_tag="PWU31_002005"
     tRNA            complement(19775..19848)
                     /locus_tag="PWU31_002005"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19812..19814),aa:Asn,seq:gtt)
BASE COUNT         6186 a         4233 c         3151 g         6285 t
ORIGIN      
        1 ttgtatttga taaagaggtg agtaaaaaaa taaaatagct gagcattgga ggatagattg
       61 agtgatacta agtctatcct cttgtttttc taaagaacta agctagaatt ttttcttttg
      121 tacgttatta tatgttattt gtcattacca aaaagcacct tttttcttct agtatctatg
      181 acataagaaa taccacccat ttcctttact tgaacaaatc ctactgattc ataaaatttt
      241 tttgttttct cagtatcatc tgtcaataat tcgatttgga ctggtccata actagtgatc
      301 ttctccaaag cttcattcaa aagcactttg cctattcctt ggcgctgata cctcggatga
      361 atgaggatat cttgaataaa tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg
      421 ataatcttcc cttccccatt acgctttatc agagattcag attgttcaaa tgctctagct
      481 aaccttgcag tgtcttttgt gtaattcgtc caaccaacgc tcttatataa ttcagaaatc
      541 tcatcgatca taagggaatc aatattcatt tttttcttcc tttcttatac aattctccct
      601 ctaataatac agttctttga ctatttaatc tatcacatat aactctcctg taaagattct
      661 ctcgattcct taaaatctct ctttcctttt agaatgcatc ctttttgata aataaaaagg
      721 ttgtgataaa ttttcttcac aacctctgtt tctctctatc aagtgttatc ttttaatttt
      781 ctacccttac cctagcatct ctattgcccg tagtatgcat actttccatg tttgcgaaaa
      841 taatgtttat ctagtaaata ttgcgggatt tcacatatat tggggttgat cagctcgttt
      901 tctttggcca tcagacaaat ttcatctaag atcaaactgt tttctactgc tttcataggt
      961 gaatcccccc atgtgaacgg tccatgtcca tagacaagta ctccagggac ttcgtttgga
     1021 tctaattttc tttccttaaa cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct
     1081 tctcttactt cttctttcgt taattggcgt gtacaaggaa ccttaccata aaaagcatca
     1141 gcatgagtcg tgccataagc tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc
     1201 gtggaatgag tatgagtaat agcagttata tcttcaaaag tttgatacaa aacaacatgt
     1261 gtggggagat ctgaagatgg ttttaagcta tcttcttcta ttatatttcc cgacaaatct
     1321 gttaccacca tttgatctgc ttgcatgcat tcatatctga caccgcttgg cttaatgacg
     1381 atgactccta gctcacggtc cacttcactc acattgcccc aagtcaattt gactaatccg
     1441 tatcgcggca atgccagatt tgcttcaagg acacgttgct tcattgctgc aattttttct
     1501 tcacaagaca tcatagccag cctccttcaa tttagaactt aagtagtctt ttgcctgctg
     1561 gatttctctt cgaaagtctt ggcttttttc actccacatt tcaatcagaa atgttccatc
     1621 ataatctaga cgtttcaaat gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa
     1681 aggaacatca cgaaactggc ctgaatattc ttttgttaca gggtaagtat cttttaagtg
     1741 gatagcagtg atataatcga tccctttctc aagttctcga gccggattat tttccggcca
     1801 tgcggacaaa ttgcctaggt ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt
     1861 gatttctaaa aattttgaaa tggagttcat aaatggatca tccatgattt ccacggaaag
     1921 aatgatgcca tatttagaag cttcctttac gctttctttt attccttcga taaagtcttc
     1981 tctggaattc attgatttct cttcataata aacatcgtat ccagctattt ggattacttt
     2041 gacgcttaga ttatgcgcca aatggattgc ttttttcatt agttgtttcg ctgctttttt
     2101 cttttctgga tcagcagaac caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg
     2161 gatacgtaca ccggagctga acatctcttc tctcagctca gctatctttt cttcagacca
     2221 atctaatcgt gctaaccgct catccgtctc atcaatcgac atctcgataa aatcgaagtc
     2281 catttctttt acaagggaaa aacgttcttt ccatgaaata tctttgggta acgctttttc
     2341 ataaatccca atttgagcca tttccttacc cccagatacg aatgatctca tctttgaagt
     2401 tttgtgcggc cttcatcgga tcatttgctg ctgtaatacc acgaccagtg ataaaggtat
     2461 acacatcgat cccttcaaat aagtgtaatg tgtgtggatt taatccacca gttacagaaa
     2521 cattaaagcc tagttcaatc agttttttga tcttcgataa atctttttca ccccaagttt
     2581 caccagataa cagcgcatca cggctttgat ggtagattac ctgccggata ccgatgttat
     2641 gccaatccat ggcttgttca aaagtccaat tgccgtagag ttctacttgc aattcaccta
     2701 tttctttttg tgctgcctcc atagttggaa tcgttgcaca acaaatgact gtcatccagt
     2761 ctgctcctgc ttttgcaaca ttactcgcca ctgttcctcc agcgtctgca catttcgtat
     2821 cagcgactag ttttttatct gggaacatac tgcgaataca gcgaatcgct ttttgtcctt
     2881 cctgtaagca aagaattgtg cccacttcaa taatatctac tacatcacca actttcataa
     2941 catctcctaa tgcatgttct aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc
     3001 tcatcttagt tccttccttt acttgttttt ttggtaccac tttgtttcag caaatttttc
     3061 actgatttct ttgactgaca tcacattttt gataccgata actgtaactc cttttttctc
     3121 tgcctgagta aacatattca aaaaattgac tggcgtaaaa actacatcgt attggccagc
     3181 tgaacttttt ccctctgaaa tggcacaatg atggattttg gtaattggta cgtttagttc
     3241 tttcattgct ttttccacac ttctcatcat catcaagctt gttcctgatc catttgcaca
     3301 cgatactaaa attctcatct taatccctcc attatgagtg ttgtctaatc agattttctt
     3361 ttatactctc tgcttttctg catatgtgtc atagtcttct acaatcaaaa aataaccatc
     3421 aggatttttc cgatattgta actgcggaat agccaataaa ccaatgacaa ctacggcaat
     3481 tccagcataa cctaggactt tcatgattac tgtaaacaat ggccaagctg ttgcccagtc
     3541 gaacatcccg atataaccac cgtatgttga catcccaatc catgtagcga ataatgctga
     3601 accgcctact tgtatgattc ctgaaagaaa cgggaacagg caagcggctt tgaatccacc
     3661 tcggttattg gcataaactg ctataacggc attatcaaag aacaacggaa tgaaaccggc
     3721 tatgacgatc gttggtgatt tcaataaaat cagtaaacca atcatcaaaa actgtcccaa
     3781 agcaccaaac aagaacccaa tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc
     3841 tacgtcaatt cctggaacag ctcctggtaa aatcgtgttt gagattcctt ggaaagattg
     3901 agttagttca tcgacgaatg tccgaacacc taattgcaag atcgccagat ataccgcaaa
     3961 atttaaagaa gtcgtcataa tgtagaataa gaaactctgc ccttcttgca tgaattgcgc
     4021 ttgaatcaaa taatctttgc ctagtacaat cagaatgatc ccaaagaaaa atagcatgag
     4081 taacgaagta gcaaccatat tctcattgaa aatcgacaag aatcctggta atttgatatc
     4141 ttcaattttt ttgtcttgct tgattgatct atttttcttt tcatcccgct ttttcatcca
     4201 ctcagctaat tttgcaaata taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc
     4261 ggcaccttct gtcaattcct gcgtgattcc tactgttaag ttagaagcta ctgtccaata
     4321 acaacctaaa atcagtccca taaaaatcaa tgcttgtatc ctacctagat caggaaagca
     4381 aaacagtaaa atccaaaaag cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa
     4441 aacggaacgc agtttcgtat atttctgaaa acggactaat aagatattta aaacaaatgc
     4501 aatcagtaat aaaatcatca catctccaaa ggtacggcca aatgtctcca ttaatccagc
     4561 atccacagca ttttgtccaa aataaggatc tgtaaccatg gcatccaaat tgaaccgttc
     4621 ctttagtcca accaagattg gccgaaaatt attgaccaaa ccaccagagc caacagtcaa
     4681 aataaagtaa ccgaccgttg cctttaagaa tccagcgatg cattcatgaa gcgggcgttt
     4741 cagcaaaata tagcctaata acacgataaa accaatcagg tatgctggct gcgtcaagat
     4801 atttgttgca aaatacgtcc aaatgcttaa caaaaattcc cccatctttt ttctcctcta
     4861 tcaattaatt atattctgcc atcacaactt ggtaatcatc gatggagttc gtagagatga
     4921 gacgctcaac caatccatct gtcattaata attcggataa tttttggata ttttctagat
     4981 gttcttctgg atcttttgct gcgagtgtaa aaaacaaact agccgttttc tcctcattct
     5041 ccccttcact ttcaaaacgg attggctgtt tcaacttggt aaatgaaata gctgttccaa
     5101 aaacaccatc acttttttct gaagaatgcg gcattgctac ttgaggtgcg atcacaatat
     5161 aaggaccgta tttttctaca caatggatga tctcctccac atacgtttca tcaataattt
     5221 ccttatcgat caatacaaga cagctttcgg cgatagcttc acgccaatcc gtgatttctt
     5281 tatctgggta atgaatcaat tgatggtcaa aaaagtattt cagcaagaaa ctccccttct
     5341 ttcttataaa aacgacggaa acggtgtatc attttcaatc gagaagacat cttcaaatcc
     5401 tcgataaaaa ttaaactcaa gatcatcttt gtttgtcgga tagatgaatt tcccgcctac
     5461 ttgccagata taaggtttaa atccatactt caggcgatcc tttttgaatt tccaaatttc
     5521 agtgatctct tttggatcag ccataaaatt agaccaaata tcgtagtgga caggaatcac
     5581 tacttttgca ttcaatgact ccgccatgcg cagcatatca acagaagtaa ctttgtctgt
     5641 tatccctcgt gggttttcac cataggcccc taaacagaca tcgattttgt gttcattacc
     5701 atgtttggca aataaattgg agtaatgtga atctcctgca tgataaagat tccctcctga
     5761 tgtttcgaat aaatagttta ctgcaatttg atccatctcc tgaggcattt ttccttttaa
     5821 tacctcttct ggatcttcac aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac
     5881 tacattgatg tctttgattt tgactgcatc acctggcttt actgtaatcg ttttttcttc
     5941 aggaattccc catttttgcc acgtgcgaac cacttctttt ggcccaatga atgttgcttc
     6001 aggacaattt tgatgaactg ctgcggctgt atggatatct agatgatcgg aatggatatg
     6061 tgtaacgatt aaagcgtcta cattttttat cgcaaaagga tcgattacga aaggctgagt
     6121 acgtaaatta ggctgcatat tttgaacgcc actcatacgc atcatctgat ggcctttttt
     6181 catcatgcca ttcccatgac tacgttttcc cgtcccacac catagatcac ataaaatatt
     6241 cgttccttca tgacttttca gccaaattcc cgtacagccc agccaccaca tagcaaaagt
     6301 tccaggatcc actacttctg tttcgatttc ttcattcaag tatgtgcccc actcaggaaa
     6361 tgtttctact aaccactttt cttttgttac ttcattgaca gttttactca tttgcttgct
     6421 ccccccatat cgttgttcca acttacaagt aaatattaat gtgtatcagc atccatgaaa
     6481 acccttttat atgaccgttt gttttttata tgattttctt tcagtaatca atgattgttt
     6541 cagtataata aagacagata tgaatgttta aatgaacaac tagtcaaata aatccatgga
     6601 tcaagatgtc cctcaggagg caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg
     6661 tcaaaaagaa attttggaac tgcttcagca gactgaacaa ctatcaactt tcgaattagc
     6721 tgaatccctt gatgtatcta ttagtacgat acgaagagat ttaaatttat tagaagaaaa
     6781 aaacgagatc atacgcaatt acggttattg ttcttataat tacaaaaacc agactgattt
     6841 tgatcaatct ggtcctgaaa ggattaaaca agcgattgcc agagaagcta gtaaatacat
     6901 taacgattat gatacgctgt ttatcaattc tagctcgact gcgttgaaaa cactggattt
     6961 tcttcaatct aaccatttga cagtcattac aaacaacgtg aaaatcaatt actctccgca
     7021 caaagataat tgtagttata ttttaactgg tggagagatg cgctttccaa aagaagcact
     7081 agtgggcgac atcgcattaa atacgatttc ttcagtcaat gcagacattt gtatcattgg
     7141 ctgtagcgga gtcgatgtag tacatggggt cacaacaaag atattaaatg aatcaacgat
     7201 caacgaaatg atggtcaaca agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa
     7261 aatcggactg acatcaaagt tcaagattgc tgatatttca gtctttgatt atttgatcac
     7321 tgataaattt tgttctgaaa aagtattaga ggagatcaga aatacgggga taaaaaccat
     7381 tcaagtagat tcattttaag aaccaggacg taaatatgta aataggatta tcaaaaaaat
     7441 gaataacaaa tttatcgata aatgaaaaac acgctacgct caaattgata aaggaatccc
     7501 aagatgtatc catccaatcg ataccatcat aatctattgc ttcttttata accgttatat
     7561 gttgcctaaa taggaatcaa acaatatcga aaaaaggagg tatgacaaaa gtcacacctc
     7621 cttttttcga ataaacggta ttcaaatcag aacactgatt tcacaaccac tttatttcag
     7681 gtcgttttca atcaccttta ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc
     7741 gtccatatat ctttctaaat aatcatcttc tcctaattgc ttatggcaag aagcaatttc
     7801 ataaagcaag tattccaatg cataagtcga tttatgtttc ttattatact caataccctt
     7861 ctcacataat tggattgctt tatcataatc ctcgatttta cggtaatagg tggcgatatc
     7921 ataatataca atgcagacca tcagattatc aaaatcagtc tcactcaata ttttaagtgt
     7981 acgctccaca taaactttcg caaaatctaa ttctcctttt gacaagtaga tcattgccac
     8041 gtttgcagct gcttgtaagt gatggatatc tgtttcggtc gtttgttcca acacttgatt
     8101 aaaataaaat aatgctgcat ctggtttttt cgcataaaga tgatcaagca gtccttgata
     8161 aaataaaatt tgatttaaaa gatcagaatt acttactttt tcctgatcga tcgtttgtaa
     8221 aattttataa gattcttggt attttgatac agaattcaat gattgaatct catttatttt
     8281 tttcctgatt ttgtcttctt ctgtttctac taatatatcg gagatctcga cattcagctt
     8341 tttgcaaagc gcacttaaga tattgatgcc tggcgacata ttcttgtttt cgatattgct
     8401 gacagttgct tgttcacaaa tgccttctgc taattgtact tgtgttaacc cctttttctt
     8461 tcggtattct tttagtaatg ctccatctac tttaatcaaa acaatttcct tccctttaaa
     8521 aaattatttc tcctttatat catacacaaa aaaaagttct aaacaaccaa aaatgtttag
     8581 aacagaataa aaattttaat tttaaaataa tattcaaatt aaacaactgg cgtatctgct
     8641 ccttgatttc tttttgtgtt atctgaataa ttatcgttta cagttataac acatagtgaa
     8701 aattgtcatg gcgagaaagt ctaagaatat ttttaaacat aactatttct ttcttacgtg
     8761 tgtataatga cgacagcaca cctatcaatt aatataatta ttttattttt ttatgttctc
     8821 atgtttattt ttaatgacac aacttctcgt atttatctgg cacaacatat aaatcgatca
     8881 aaaaattgtt gcgtgttttt ttgaaaattg gagtttcaac ttgattacaa aatttaaatt
     8941 tagaagttgt gaaaacagcg agagacagtt taatattcaa aatatacata ggctactcga
     9001 acgctgtgtt ttttagtata ctagtaagaa acgaagggat agactcattc caataaggag
     9061 gatttcatga agacaattct ttttgctcct gcagtattca atttagctga gacaacacga
     9121 atgattgagg ttgccaaagc tatgaaagac cgttattact gtgtattctt tggcttttct
     9181 aaaacttttg ctcctttgat cacagaaagt ggctttgagt atcatttact agcgccgatc
     9241 cttacgaaaa aacaagaaga acaaatcata aaatttgacc agttgcgtac acttaaaaac
     9301 ccttttacca aagaaatggt tgcctctcgt atcactagtg aacagagtct gattgctaag
     9361 tttgagcctc gactgattgt gacaggttcg aatgtgacaa tttttttatc ggcccgcaat
     9421 aaaaagattc ctttagtata tgttaaaccc tatgctttga gtgaaccttt ttttcaatca
     9481 gagcatgcgc tgatgcccca cgagttacgt ttcttcggcc ctttaagcaa tcccctttgg
     9541 cactggacaa aaaagcgtat agtaaatacc cgatggatcc ccaatgcctt caaacacgct
     9601 gcacaaaaag aaatgcttcc tttaccaaaa acaagtttac aaatgatgga tgctgatcta
     9661 aatctcatca caacgcctaa actatttttg gaagaagaac agctgccgga aaattatcga
     9721 atcgttggtc caatttttgc taagctcagt acttccttgc ctgaagaagt gctaactttt
     9781 atacagcgaa agcaacaaga aagaaaacgt attatctatt tcgcaatggg tagttcaggc
     9841 aatccgcgta tgttacgcaa aattttgttc ttcttacaaa aacaaccgga tctagcggtt
     9901 attgcaccta ttgactatct gcttgatgac attcaatctg actcagaaga tcttttaata
     9961 acagaatatc tgtctagtca tcttttgcat gaattcattg atttttcttt tatccacggc
    10021 ggggaaggga ccgtccaaac tgcctgtgaa tccggcaaac cgtttatcgg tgtggggatg
    10081 caatttgagc aaagctgtaa tattgactat tgtgtaaaat acggaaacgc aatcgccctc
    10141 acaaaaccag tgactgaaag aaaactgcag attgctctgc gcgaagtttc ttcacccaag
    10201 attcgtcagc aggcacgcac gctgcaagcc gtttttccaa taaatggtcc acaacaagca
    10261 attcaagaaa tcgaaacctt cctgagcaac cacccaatat agtttcaagt agaaaaagct
    10321 tttttaatag agactcatca gtaatttcgc tattcgagac ccagacagtg aaaatacaaa
    10381 aaaagtaaat agtggattga aaagttagag ttacttaaat tacggtgtct aaaaaacaca
    10441 aaatcatcct taaaataaaa gatctgacgc tatccaaatg agcataggta gcgtctgcca
    10501 tttattctta gtcaaattct ttgacacctt tctctggttg gatcatctca acgtttcttc
    10561 aaaagaaaga ccataataat taggcaaata cattttctta aaatgtgtca tatagatcta
    10621 gaagcatcgc tttgtagaga tttgtgccgc tagtcccctc aacaattact aaatcaaatg
    10681 taggatttac cactttatct ttgataatca acctccctct tctgttgaga caatgatctc
    10741 gcaatccgat gcatactcct atataaactg ccagcaatcc ccacatattg agtagatcat
    10801 gtcagaatgt tctatgtcgt cagcaacgat acactaaaag tcacgttcac catgactgat
    10861 tgctattccc agcacaatta tttctataca cgtttcatga tttgaataaa cactcacatc
    10921 atacatatta ccattcatac accatactgc agcccctact atatggttgt aagctcccaa
    10981 atctatattt gacttgatca ctttggtagt agatctacta attctaaatc cattgctgat
    11041 aaaatttcca ttttttcctc cttctttctg ccaaaacagt taaaacaata ataatcttta
    11101 tgtaaaaaag ggctgtgaca aaagccttgt cacagtccca tattccgaat aaacggtggt
    11161 caaaagcttg catctgcggt aataagttca accaaacgaa aaataagcca acatttcaca
    11221 aaaatttaag aagcaatttt cgtaaatttc ttcttatttc ttgaagctaa ccacttctgt
    11281 tacaaccact tttagaacat ttaaagcttc gatgtgacaa accatctatg ctgctcgatc
    11341 ataaaattga tagcaaaaat tccacaaagt acatatgtcc cccatgaaaa caaaacattc
    11401 atatgcggta caatgatctt tggaagaatc acgatcaaaa atgatgccag tgcccaaatc
    11461 catcgattga tttgaatatg atatgtagct agcatattca atataaggtg actagccgtt
    11521 aataaaaaga taaaaacaaa aaataaaatc agccaataca ttgtttccca ccttctaaaa
    11581 accaaactta gacagtctgg acttcgtttt ctccacgact ttgttcatct gacagcatct
    11641 ttttatcata agcttttaca aatggataat agataataaa tgctatcaat gtgttgatca
    11701 tgatcaagat aacaacacgc cagtcatttc ctgctgaaag ataaccgccg attggagctg
    11761 gtaatgtcca tgttggcaaa acagtaaatc tattgaccat ttctaatttg actgcaaaat
    11821 aactgataat agtcaaaact aaaggaacta agttgaacgg gatgaacatg taaggattca
    11881 acatcatagg agctccaaaa attatgggct cattgatatt gaagatacca ggaataatag
    11941 aaaaacgacc aacctctttc aagaattttg atttacagaa gaatataaac aaaacagata
    12001 ccacgatcgt tgctcctgca ccaccaattg ttaccgtcca ttgataaaac tgttcaggag
    12061 cgatataggg taattgggtg gcagctgtac catctgccaa tgcctttgca ttctcatcca
    12121 acatgatcag ccacattgga cgaacaacag cccctacaat actcatgcca tggataccaa
    12181 atgaccacaa caaatggatc aaaaacattg gtagtaaaac accaagcaaa ttattcccta
    12241 aaatattggt taaaggctcg aataacgata ataaggcagc gtttacatca aagcccgcga
    12301 cgcttcgaat cagccaagta attgttaaaa tgactgcacc tggaatcaat gcctcaaaac
    12361 tacgggcaac agcaggtggc acttgttctg gcattttgat agtaatattt cgatttttaa
    12421 agaaacgata tacttccact gcaaaaatca tagataagat ccccgcaaac atcccagatg
    12481 ctcccaaaga actcataggt aagacccagc caagaggtgc ctcttcagga gttaaaacgt
    12541 ttacaggaac ttgtaacata aagaacgttc ctaaagataa tataccacct gtcacactat
    12601 ctaatccata tgatttagct agatccgaac ccataacaaa agttgcataa atagccatca
    12661 atcccatcgt catcctaaag ggtaaaacaa tcgttgccga ataaggggca ataaattcat
    12721 tccatccagg aaacggtaaa ttgataaaga ccataaaaaa tgtcccaatc aaaatcaatg
    12781 acaatgttga aacaacccca ttacgaattg ctaataaatg acgctgtgta ccaatttttg
    12841 ccattggtgg gacaagtttc tcttcgaccc atttcatcag cttttccact aaaaccactc
    12901 cttttcaata aaatatgaac cattcattgc atgacaacgc aaagaatgtt cataactaat
    12961 tatataatta agaaatttta tttcaatata ttctcttaaa taatcaaatt tatttttaag
    13021 aatcctgaaa ataagttttc taaatcatag gagcaacaaa aaaattcgat aattaacaaa
    13081 aatcaaaatg attaaatcta gtgattgaaa catatcattt caaaaaaatt ttgtaaattc
    13141 acccataaat atcaccctat ttaacttttt tcatcgtaca ctaaaattca aaacaactat
    13201 ctttaatctt tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa
    13261 ctacaatgat caccgtatct ttttgtgctt gctccaactt cttcaaatct ataatagcta
    13321 acagtgtccc ttcttttacc tcctggttac tttcaacccg tatctgaaac ggacttcctt
    13381 cgagttcaac tgtatctaaa cccatatgga tcagtatttt tctgccagat tcactttcga
    13441 tcgttatggc atgtcgggta ggaaatatat tatcgattat tcctttaact ggcgcatgga
    13501 tatatccatc atgattcgtt atagcgtatc caactcctat catttttgaa gaaaagacct
    13561 cgtcttttac tgctgttaat ggaataattt gaccactagc tacagctttt agaatgtttc
    13621 ttgttttaaa gaaatgcact ttttatcacc tctactttaa tgctggccaa tattttttgt
    13681 ttactttgat catttcatct agtatttttt ttgcgactct ggcatttggt acggtttgat
    13741 tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc aaagtaagca tctactagta
    13801 acttctctac tgcaacttgt gcctccatca atcctttatg aaaatcactg attggttttc
    13861 ttagtgtaat tggctccaca ccagttgcac caacatatgc cggtatttca acaactgcat
    13921 ctgaccgtag attcggaatc gccccttgat taggaacgat tagcataaag cgacgacgat
    13981 catcattcaa caaggaaata gctatatcga cgatatattg tccatgctcc ccaaaattaa
    14041 agttcaatac ttcttccaca ataccgcggc gtattttatc agccatttct ttggtatttt
    14101 tctctcgtcc atccatgacc atattagcac gtgtatactc tttgtcagta tgctcaacga
    14161 ccatatcagg atataagtaa tactctaagt aattattcgg gatattactt ggaaaatttt
    14221 tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt tttatctcct atattaaaat
    14281 cggcgacttg catttctttt gttattagtt tttcaataat ctctggcata atttctcgtt
    14341 gcaattcttt atcataaatc gatgtatacc agccaaagtg gtttaaacca taatacgtaa
    14401 cgatccaatt cttacgatca taaccgtaat tcactgcaat cgtttcttca attgaaatag
    14461 tcatatcaca ggcattaatc atttttacat tcggatattt acgacgaaca gcttcagaaa
    14521 cgatcgactc tggatttgta taattcaaaa tccatgcttt gggggcatag tcttgtatat
    14581 gatcgacaat ttccaataaa cctttgattg agcgcatccc ataagcaaat cctccgagtc
    14641 cacacgtttc ttgtcctact aatccatgtt taagtggaat cttttcgtcc ttttctcgca
    14701 tctccaatcc tccaacacga atttgtgaga aaacaaaatc gcatcctgta aaagcaagct
    14761 ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc agcataaatt
    14821 taacaatcaa gtacatatcc atatttcttt cttcatcaat atcatataaa cgtatttcag
    14881 ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac gattcctggt gtatacgtac
    14941 tcccgcctcc agctatagtg ataatctgtt ttttcatcat catcttttcc tttctattct
    15001 tctaataatt tattgaattc tgccataaca taagtcactt ctaagccgat gataatttga
    15061 taattttttt cagttggttt aatgatcccg cttatcccta attgcttgat tcgctgttcc
    15121 tcgaccatgg tgggatccac tacctctacc cgtaggcgtg tattacaact atagacatcc
    15181 aggatattgg cttttcctcc aaacgcttgt agcatttttg ttgccatata ttcataattt
    15241 ttacttgcta accctaactc tgtttcttct aaggttactt cattgccaaa ctctgcctta
    15301 gcttcacgtc ctaatgtctg aatgtttctc ttattgatca aataataaaa agtaacgtaa
    15361 tacaaggcaa aaaagaaaat tccgattgga agaatcaaaa tagcattggt agcgattcga
    15421 aaattgatga gataatcaac aatacatgct ccgaatgtaa aaccaattcg tatattgaat
    15481 aagtaacaga caatccctgc taaaccggca aaaagtgcat ggatcacgta caacatagga
    15541 gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa tgaagttcct
    15601 gcaccgcttc ccatgattcc tttgaccatc tctttatttt ctttataagc tgctttatag
    15661 atagctagac atatagctgg tataccaaac atcattacta caaaaaaaac tgataaaaac
    15721 agtccagcct gaggatctcc gttgataaag cgtgtcatct ctcctctgaa aacttccttg
    15781 ctaggagatg tatagcttcc taaatcgtaa taaatatacg tattcaacac atggtgcagt
    15841 ccaaagggga tcagcaaacg attcaaaaac ataaatatcc ccacacctaa tgctcccata
    15901 tcaacgagca gttttgcaaa attatcaatc ccaatttgga tgagcggcca gataaagccc
    15961 aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa tttttcgccc
    16021 gcaaaataag aaaaaactac tggtaatttt tgttctttaa agcgattata cgtccatgcc
    16081 gctactaaac cggaaataat tccaccaaaa atccccatat tcacttctgg gtccataatt
    16141 tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg tattcctttg
    16201 tccttatttt tgacaaaacc gattgcaact cctatagcaa ataagatatc caagtttcca
    16261 aaaacggcat ttccagctgc accaattact gggatgttca gcatatcgtc agctgataaa
    16321 cgcatgatca atcccgctaa tggcatcgct gcaactggaa gtagcatcgc tcttccaagc
    16381 ttctgcacat ttgatttaac attcattttt attcctcctc tattaaatat cctcatatgt
    16441 tactaactca atcaaaggat catttttcac tttttgagca aatgctgaat gaattaaaaa
    16501 ttcgacttgg tgggtacgag gatacacaaa acttgaacca tttaacaatt cctgatccaa
    16561 atacgctgcg tgacacatga cttcgatcgt tttatcttct ctcaatatca tgagtaaact
    16621 tgccaaatat tcttccacac tcgtattgat ttgtcgtttt ttttcatcag tttctcctac
    16681 gacatcgaaa caaggctcaa aataatcgac atgacgaact tcatcttttc gttcaaagtt
    16741 gctccgaacc ggcaaatcat atttttttgc taatgtaaca actacctcct gattttcatc
    16801 caaagtatga gtatgatgat gactgtctaa atgagttggt atgatccccg catgataaat
    16861 tttttgaatc tgagcattcc attcctgata cagttgatct ttgtcaatgt taaatggatg
    16921 ctgataataa gataaggatc tgaactctcc gctttcgata agagtatcga cagttttgag
    16981 gagtggtttg ccacacgtca atgttaaatg gactcctaca cctagcctag gcatttcctt
    17041 tcttagtttc acagcatgct cgaaacccgg catatttgcc atcaatgtag ttgatgtcaa
    17101 aattcctcgc tgataagcat ccataatccc ataattcaca ccatggctat agccaaaatc
    17161 atccgaatta aaaatcactt ttcccatttc attctccttc ttttttctac taaaataggc
    17221 gcaaccaaat aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta
    17281 cgcccttatt ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt
    17341 gaatggcaag ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg
    17401 ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct
    17461 gataaatcaa gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg
    17521 attctttatc ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca
    17581 tttctactat ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat
    17641 gcatagtgat gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc
    17701 gctcgaccac ttctactgct atatcatatt catttggata aaggaatctc atttctggca
    17761 acataattgc tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat
    17821 ctgtcaatgt aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt
    17881 caatcacttc ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga
    17941 atcgtttgat cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa
    18001 atggcttact cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc
    18061 catcgtcatc gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt
    18121 cacctcctct atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct
    18181 ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct
    18241 aattgaatag tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt
    18301 atcaatatct tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat
    18361 tacataaaaa caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta
    18421 taccggcggc cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg
    18481 cgtctgccaa ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg
    18541 aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata
    18601 aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc
    18661 acttcgccac attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat
    18721 gaagacatct tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact
    18781 ggattttgag tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac
    18841 taaggcggta accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact
    18901 tggctatacc gccattatac tattatcaat ctataaaata aagaataaat tatagaaact
    18961 ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg
    19021 ctatacccca agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca
    19081 caatccggtg cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac
    19141 ccacactaac ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga
    19201 ggagagtgga ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc
    19261 cacttcgcta ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt
    19321 caatccgttg ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa
    19381 caagaacgac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg
    19441 ttaaccccta caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc
    19501 ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat
    19561 gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg
    19621 accaacgggc caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt
    19681 ggcaccatct acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt
    19741 gaaagaagct caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg
    19801 acatcatgat taacagtcat gcgctactac caactgagct atgccggaat aacat
//
LOCUS       DANJMA010000046        19646 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000046 DANJMA010000000
VERSION     DANJMA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            133..3660
                     /locus_tag="PWU31_002006"
     CDS             133..3660
                     /locus_tag="PWU31_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL1084507.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PWU31_002007"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PWU31_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL1084508.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PWU31_002008"
     CDS             7448..7789
                     /locus_tag="PWU31_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084509.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PWU31_002009"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PWU31_002009"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL1084510.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PWU31_002010"
     CDS             9146..11566
                     /locus_tag="PWU31_002010"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL1084511.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWU31_002011"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWU31_002011"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL1084512.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="PWU31_002012"
     CDS             13000..13737
                     /locus_tag="PWU31_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1084513.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="PWU31_002013"
     CDS             13751..14566
                     /locus_tag="PWU31_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1084514.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="PWU31_002014"
     CDS             14579..15226
                     /locus_tag="PWU31_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1084515.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="PWU31_002015"
     CDS             15242..15898
                     /locus_tag="PWU31_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1084516.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="PWU31_002016"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="PWU31_002016"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL1084517.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="PWU31_002017"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="PWU31_002017"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL1084518.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="PWU31_002018"
     CDS             18240..18758
                     /locus_tag="PWU31_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1084519.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="PWU31_002019"
     CDS             18865..19365
                     /locus_tag="PWU31_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1084520.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6587 a         3375 c         4215 g         5469 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaatt
//
LOCUS       DANJMA010000047        19370 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000047 DANJMA010000000
VERSION     DANJMA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            278..544
                     /locus_tag="PWU31_002020"
                     /pseudo
     CDS             278..544
                     /locus_tag="PWU31_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1927..3093)
                     /locus_tag="PWU31_002021"
     CDS             complement(1927..3093)
                     /locus_tag="PWU31_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL1084521.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            complement(3181..3465)
                     /locus_tag="PWU31_002022"
     CDS             complement(3181..3465)
                     /locus_tag="PWU31_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084522.1"
                     /translation="MSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANT
                     LVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            complement(3695..5119)
                     /locus_tag="PWU31_002023"
     CDS             complement(3695..5119)
                     /locus_tag="PWU31_002023"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL1084523.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            complement(5487..6503)
                     /locus_tag="PWU31_002024"
     CDS             complement(5487..6503)
                     /locus_tag="PWU31_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HDL1084524.1"
                     /translation="MKMKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQL
                     KEDRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAY
                     IDSNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKK
                     SNELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKME
                     RDFTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFV
                     ETIEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTV
                     YYLS"
     gene            complement(6749..7687)
                     /locus_tag="PWU31_002025"
     CDS             complement(6749..7687)
                     /locus_tag="PWU31_002025"
                     /inference="COORDINATES: protein motif:HMM:NF016640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HDL1084525.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            complement(7724..8815)
                     /locus_tag="PWU31_002026"
     CDS             complement(7724..8815)
                     /locus_tag="PWU31_002026"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HDL1084526.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLASTS
                     IVFLLISNNFIRIIQNIFVLNYIIYASVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFI
                     NIRGTAFVNGILPLFRENIIFKAVYYFFE"
     gene            complement(8787..9683)
                     /locus_tag="PWU31_002027"
     CDS             complement(8787..9683)
                     /locus_tag="PWU31_002027"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1084527.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(9673..10833)
                     /locus_tag="PWU31_002028"
     CDS             complement(9673..10833)
                     /locus_tag="PWU31_002028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084528.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNA
                     EKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGIN
                     YNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
     gene            complement(10826..11440)
                     /locus_tag="PWU31_002029"
     CDS             complement(10826..11440)
                     /locus_tag="PWU31_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL1084529.1"
                     /translation="MYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGSPVIFK
                     QQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPELWNIL
                     KGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFEKDIEY
                     VENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(11448..12530)
                     /locus_tag="PWU31_002030"
     CDS             complement(11448..12530)
                     /locus_tag="PWU31_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL1084530.1"
                     /translation="MSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEEELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPVFVDSDYETWNMN
                     PEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGTDVAQTLFEKGIC
                     LPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(12568..13200)
                     /locus_tag="PWU31_002031"
     CDS             complement(12568..13200)
                     /locus_tag="PWU31_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1084531.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(13211..15028)
                     /locus_tag="PWU31_002032"
     CDS             complement(13211..15028)
                     /locus_tag="PWU31_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1084532.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(15041..15805)
                     /locus_tag="PWU31_002033"
     CDS             complement(15041..15805)
                     /locus_tag="PWU31_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL1084533.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(15833..16531)
                     /locus_tag="PWU31_002034"
     CDS             complement(15833..16531)
                     /locus_tag="PWU31_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL1084534.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(16543..17322)
                     /locus_tag="PWU31_002035"
     CDS             complement(16543..17322)
                     /locus_tag="PWU31_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL1084535.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(17338..18282)
                     /locus_tag="PWU31_002036"
     CDS             complement(17338..18282)
                     /locus_tag="PWU31_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1084536.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(18328..19107)
                     /locus_tag="PWU31_002037"
     CDS             complement(18328..19107)
                     /locus_tag="PWU31_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084537.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         6041 a         3705 c         2828 g         6796 t
ORIGIN      
        1 gtataaaaaa atatagaaaa gacaccctga aatcctttag aattaatttg cccaacaaat
       61 tcataggagg attcaagatg tctcatactc tgcttatcaa agatacccta aatattctag
      121 acctaaatat caatatccat ttcgaggaaa attgtttaac taaagaaaaa tacaaaggac
      181 aaatttgctt gatttacaaa gggactcttc agtacacacc agaagaatat ccacatcttc
      241 caatatatta aaagaacctt cttgaatcat tgagtgaaag aaaagagatt cgtcactatt
      301 tgaggactgt ttaactagag acattagcga tctgcctgaa acctatacaa ccactttacg
      361 aacgtttaaa aattttctgc cacagattca aaatgcgctt aactgcagct attcaaacag
      421 accattggaa tgtttaaaca accacatcaa agtattaaaa cgcaatacgt atggctttcg
      481 cagcttttac aatttcaaac tacggatcat gattcgtcat ggcaaaacat ttttaaccaa
      541 ataaacagaa gaatgtggga taaatctaaa aatcagtttt gctacagact ctcgagtaaa
      601 cggcgggagc agaagcaact ccttcggaaa taagccgaaa ttcacaaaaa tttgaaaagc
      661 aattttcgtg aatttcttct tatttctcgg agttaaacac ttctgtccca gcctcttctg
      721 tccaaattat tctaagattt ctctttacca acatcatttg acaaagagct tcttttttga
      781 atactatatt cgttctgttt ttcatcttca aatacgaact catccgcaga aatatttgtc
      841 acaactccat gaatcttctt ttcattacca cctatatacc acaggattcc tacacagaaa
      901 caacaaaaaa atagcataaa ataggttttc tttattttat atccctcctt ttagattaaa
      961 agctcacaga aaacgctttc taaaaagaca atagaataat cataattttt cattttgtat
     1021 tatttataca aataatacgc atgttactga aaaatgatat ggttccctac tttttgagat
     1081 aagttaaata aaatctgtca tcgataaaac agctagtaca gtaatgataa atagtaagaa
     1141 cttaaaatat tttggatttt tatttttgaa ataatcgaag aaaagtaaaa gtgataacga
     1201 aatagcaaac aagattatat ctataagatt attcattgag aaatcgcccc atatggaact
     1261 ctattttgac ctgacacacc gagacataaa gcaatcatat cctctgcatt gttttatact
     1321 attagaacta tagctacacc tattggatgc tactggctct aagattaatg ctaatacagt
     1381 aacatcaata cctaaactag cagaaactct atttgcatct tcaatttcag atgcctggac
     1441 tgtcgtgact agcgccgtag tagaaaaatt tactggtact aacaaagatg cagacacgac
     1501 ttttatttca cttatccttt atcccataac ctaaacttca atcattactt gtgagctgat
     1561 caatgttctt ccaagtctga aagaacattg atcagcttgg caaacaaata atgttttcaa
     1621 atacttgatc gatgaaaaaa agctattggt aataacagta attgcatttt ccattttcgt
     1681 gtcagatact tcgcttacag ctggtaaacg atcaatccca ttgtcaaatt ttggattttt
     1741 ttctgatgat gtcttgcttt agttcataaa ataatccagc aataaattcc tcgcaaggat
     1801 agccaacttc attttcctaa tcggaatttt taaacttatc ggtatagata cagatgcaat
     1861 gagaacaaac atttatttcg agtcaccatc taaaatattt tcctccatgt acccgttcat
     1921 tattatttag ttatcaccaa agatatctct ggtataaact ttttcttcaa catcctttaa
     1981 aatatcttca aaccgattag atacgatcac atcagaaata tttttaaatt cattaatatc
     2041 tttcaatact tgtgaatgat aaaattcatc ttcatccaat gtaggctcgt aaacaaccac
     2101 ttcaattcct ttggctttaa tccgtttcat aattccttgg atcgacgaat atcgaaaatt
     2161 atctgaaccg gccttcatcg ttaaacgata gattccaaca acatttggtt ttttcgccaa
     2221 aatctgtcca gcaataaaat ctttccttgt tgtatttgca tcaacaattg cactgatgat
     2281 attgcttggt actttttcat agttggctct taattgtttt gtgtctttgg gtaaacagta
     2341 tccaccatag ccaaaagaag gattattata atgcgaaccg atccgtggat ccaaaccgac
     2401 accttcaata atttgcttac tatcaagccc acgaaattct gcatacgtat ctaattcatt
     2461 gaaatacgcc actcgcaatg ccaaatatgt attcgagaat aatttaatag cttctgcttc
     2521 agttgagtga gtcaaaagaa ctgatgcatc tttgttaaat gcgtttcttt gaaacatagt
     2581 tgcaatctcg cgaccacgag cagaacattc tccaactata attcttgatg gatataagtt
     2641 gtcgtacaaa gcttttcctt cacgcaaaaa ttcaggtgaa aaaatgatgt tttctgtacc
     2701 aaatttcgct ttaatttcat ttgcatagcc aactggaact gtcgacttaa taatgaatgt
     2761 tgaatctgga cgaattgcta aagccttctc aattacatct tctactgttg atgtattaaa
     2821 atagtttttc ttttcgtcat aatctgttgg agttgcaata atcaagtaat caccgaagtt
     2881 tactgcatca gtaaaatttg acgtaaattc gacatttaag tcatcccgtt ttaaaaattc
     2941 atgaacttct ttatcttcta ttggtgagat gccattttgt aacatcgtga ttttttcttc
     3001 cacgatatca taagctttga cctgctcttt ttgtgctaat aatagtgaat ttgctagacc
     3061 aacatagcct aatccgaaaa ctgaaatttt catattcttc catacgctcc aatcaaattt
     3121 cattttatag tattttagct gttatccttt tgaacatatt ccttattcaa tcaatattat
     3181 ttaatagaaa aaattttttt gcttcaaaat catcgaggtt cgatatttaa taaattcatt
     3241 tttatttttc tcataatgct cctctatagt tgaaatcact ttacatgaac tacccgctac
     3301 aacactatta tttgggatat ctttatttac taaagtgttc gctctaataa ttacattatc
     3361 gccaattgaa actcctggga aaacaataga gtaaatacca ataaaaatat tatttcccaa
     3421 tttgatccta cctatttttg aagttgactc gctcacagaa ctcatcttcc ttaagaccca
     3481 gactccacca tcgtgtgtta taaatttaac accaaatgaa catctgaaat tatcccctat
     3541 ttcaattaaa tatggctcgc tcccaaattc aggaacctct actaaactat gtttttttcc
     3601 gaatatcaaa ccttcattaa tacattctcg cagatttttt ccaaatagtt ttctctttat
     3661 tcttcgaaag attctattct tacttcgtat accctcacct tttagccatt tttattctga
     3721 aaaatttttt tagatacgtt ataattaatt gtttatacgt aaaaacgaaa atagcactaa
     3781 aaataatcgt taagaaaaga ttaaaataga agcctctatg aatgcataca aaagcgattg
     3841 cggaatagag aactaaaagt agcgatattc gcttccaatc aaatgcataa taagcaattt
     3901 tccttccaaa ataatatcta agtccaaata atactaaagt accgagcaaa gtagcaaatg
     3961 agacaccaat tactccaaaa gaatagccta ataaataggt gccaacaaca ctcaatagac
     4021 ctgacactac tgaaatcatt aataactgat ttgttttatt gtatactgca aatatatttc
     4081 cgatatttgt tgtcatccca ctgaaacatg ccataataac taatattgga actagaacta
     4141 atgatttttt ataatcaggg ttggttataa aagagtatgc acagcaaatg ataggaatag
     4201 aaagagtcgc taacctaatg tacattttaa aaatataggt atttttctca acgaatcttt
     4261 cgatgaaatc atcattctca gacgtgataa ccgcatcctc tatcatagac atactaacaa
     4321 tacttgtaag agttaaaatt atagatgaaa atttcatcgc aaaagaaaaa ataccatttt
     4381 ctgttactcc gatgtacgtt ccaatataaa atcttgaaaa accaactatt agccatccaa
     4441 aagtgagatt caaagtgagt gggatagagt atttcagcat ggactttaaa aataaaaaat
     4501 caaaagaact cgttgcgaaa tattgaaaga tctgccccct gaaaatcaaa aaaatcaaat
     4561 ttataacatt agaaattatg atagagagta agagaccaag tatccccata cccattgtaa
     4621 caacaaaaat agtaaggagt aacaggttga tacccgcagc gattatacca ctcatcacaa
     4681 aaatctttga ttcacctagt gctcttacaa tatattgaaa aacacttagg ataggagtga
     4741 aaattaattg aaaaacaaac aattgataat actctaaatt cgggaaaatc attctatata
     4801 ttattggtaa aaaaatggct aatatcactg tatttgctat aacaaaacaa atactgttcg
     4861 tcactacttt tttctgaata tttttttctt tgcctaatcc catgcgtaaa attgcttccc
     4921 atattgctcc aaaaataact ggagaaagaa attgagcaat tgtattttga taatcaaagc
     4981 ttcctaaatc ggcaacagaa atatatactg catagatagg gattataatg acagatatca
     5041 tttttgatga taatgttcct attgcgtaaa taaaagcttt tttagctatt gaatgtgatt
     5101 tattactgct actatccaac cgtatgacct ccctcaactc acatctatag atgactttct
     5161 ggtttaatta gaactaccta aattcctctg tatcgtgtga gtcctcgtag taaaaatttt
     5221 tttgaatatg tgaatagcta agatcttttc aaataaagat atccatgatc aactatatat
     5281 ttcttcaatc tcatcaatca atattggcag tcagaatcca gttcacacat cttgatacac
     5341 aattttgttt tatagaaatt cttttagtta tagaaaatat ctattcggcg agaaagttcg
     5401 tcacgtttga aagtagaatt tcctaaaata cctaacaaag ctaaaaaaat cgtatgaatt
     5461 acccatcttc aatattcctt tcaaaattaa ctcaaataat acactgtata ccattcaacg
     5521 aattttttaa tgccctcatc caaggttata ttcggtttaa agttgatttt ttcctctaaa
     5581 tcttcaatat cggcataagt tctaacgaca tcacccggct gcatgtccaa aaaaatcttg
     5641 ttcgctttaa cacctaaata cttttcaatt gtttcaacaa actccaacaa tgcaaccggc
     5701 ttattatttc caatattgta tatcttatat ggtgcccaac tttcactact caaatcatcg
     5761 atgtctgtcc agtcggagtt gggttcaggt actttattga ttaatctagt gattccctct
     5821 acgatatcat cgatgtaagt gaaatctctt tccatctttc catggttaaa aatcttaatt
     5881 tcttttccag ataaaatatt cttggtaaaa gagaaatagg ccatatctgg tcgcccaaat
     5941 ggaccataga cagtgaaaaa tctcagccca gttgttggta ttttataaag gtggctataa
     6001 gtatgagcca ttaattcatt cgattttttg gtagccgcat aaaggctaac tggatgatcc
     6061 accgaatctt ctgttgaaaa cggtactttc gtgttcccac catatactga actagatgat
     6121 gcatataata gatgctttac agggaagttt cggcaggctt caagaatatt aagaaaaccg
     6181 ataatattag aatcaatata agcataaggg ttttcaatag aatatctaac acctgcttga
     6241 gcagctaaat gaattactat gtcaaaacct tcactttcaa atagagaatc taatttttct
     6301 ttctctaata gatccattct gataaattta aaattattat aatcgtacag tatcgttaat
     6361 cgatcttctt ttaattggac atcataataa tcattgagat tatcaatcgc tacaatttga
     6421 ttttcagttt ccttgagtaa ttttctagac aaatgaaaac caataaatcc tgctccacct
     6481 gttattaata ttttcatttt tattcatcca cctttttctg aaaggaacca atagggttaa
     6541 acccaaaatt gctttcttct ttatactcgt attacattta ttatttaggc ataccccata
     6601 atttaagata taacgaagtt tatgatacta tgcgtcttct ccattcactc aaaaaatata
     6661 acttaatttt ttatgacttt aaaaaggaaa tcttctatct ccaaatattt actaggattt
     6721 gaaagaatag tttagaaatc atttagggct aataatgact tatgattctt aatttagcgc
     6781 ttttatatac attattgcct aaggtagtca cttccttcat tgcatgtccg gacttccaca
     6841 acacgtaagg aaatgcgagc tgatctcgcc cactttctga gttcaaatac tctctccacc
     6901 acaaatctag aagattctca gcgattgaat tatttaaatc aactaaaata accgtggctt
     6961 cagccaatcc aaaattatca ggaaatcctt cttctctgta cttactaatt tgctctataa
     7021 tactttcctt attacctttg ccaactgcaa ttaatgcatc tgcctcagaa tataaagatt
     7081 ttctttcact atgtttatgc atacttaacc caagtttgga agaactgttc agtaatccgg
     7141 taatatcaga agttattgtt acattaccat caacatacaa agtaaaatcg tagtcagtaa
     7201 atagctcatt aggatgaaat ttaatatatc tattgatcaa aatattattt tttaattccg
     7261 atatatattt aggtatctct ctaatattcc aatgctcact tgtaatcaca ttatctgtat
     7321 acaaatagta atctaccaat ggatgagaaa aatccggttc tttaatagaa tcataattac
     7381 cagtgataca actatacaca gcaatccttt taggtgttcc ttgattagtt aactcttttt
     7441 ttcttagttc atcactgcga ttctctctgt ttgaatagtc tttaataggc cccttaccgg
     7501 tcatcgattt aagggtatat tttgctggca acagattatc tcgttttttc atttggttaa
     7561 gttgattaat tttatttcgt attttcatgc ctaaaataga attatttttt tgatttaatt
     7621 catgtatttc aagatttctt ttttccaatt cattgatagc taaactatat aattcttgtc
     7681 ttcccataaa tccctcatct ctatttttaa gtgctattta ttcttactca aaaaaatagt
     7741 aaacagcctt aaaaataata ttttctctaa ataatggtag gattccattt acaaaagcag
     7801 ttcctcttat attgatgaat atcaaacaca caatgataaa aaagaaatac gcaagcaagt
     7861 agatataatt ctttactttt gttctcttat aattactcat taatacactg gcataaataa
     7921 tataatttaa tacaaatata ttttgaataa ttctaataaa attattactt ataagtaaga
     7981 aaactatact agtagaagct aacatattga tagaaaatat atatgagctt aagctgataa
     8041 cttttttttc gtaaatattc atttttgata atttatgaac taaaaaaaga tttgctgaaa
     8101 aaaagagagc aaacatcact acgtgagaaa tcgatgtact gtttctatca aaatatgcat
     8161 catacttcgt atttataaat attgaactga tataactttg tccaatagag aataggagca
     8221 agctaaggat tgtaacatat ccagtaatct ttttattaat ttttacaata aaaaaaggga
     8281 gataaaaata tgcaagatta tggaacagtc cagccactaa gataaaaaaa atatacttaa
     8341 atatagcttt gctgcctcgc aatttagtta gatgataaaa agctagtaat atgaatatca
     8401 atgcgctaaa catacgtact tgtattgctt gtgaagtaaa accaaaaata aaaaacaaga
     8461 acaaaactaa aaaaacatta gttgtgaata atctgatgac atacaataaa gctgagagta
     8521 agacagtaga aataatcaat aaaaaaatct gatactgact ggaaataaag gtacatacgt
     8581 acataacagc tctgaatccc ggttcaatac tatctgaact aaagtccata ccaaattgtg
     8641 ctatcccatt atacaagaca gaatatgcat aataatcagc gttttgtgtg ttaaatgcca
     8701 tgatgatcca cattatcaaa agtagcaccc cagacaaaat cttgttcgat gtactccatt
     8761 ttttttgtga aacactcttt tcttgattaa tctccaatga cgcctctgta tccatctcga
     8821 acccccctta tagccgctct aaatctactg agcttatcag tctcaaaagc tattgcaata
     8881 aaaaatttca ataatctttt attcataagg cgggaaaatt ccttatcact tttatacatt
     8941 cttttcacca atgttgaatt tcttgcaata aaatactttc gtgttgaatt atgattgtct
     9001 attttaattt tttttgatcc tatttttaaa ttttctccat ctccaacgtg atgataaaaa
     9061 tagacttgat ttgttacata aatgttatag ccattatttg caaccctttt acaaaattca
     9121 acatcaatct gatcaataaa aaattcatcc ttcatatttc caacatcgtc aattaattca
     9181 cttttgaaaa acattccaga ggaatttgca aatgctattt tcatggaacc actagttgaa
     9241 gcaaagtgtt tttttacgta tctccctctc tcatttttgc aggtaaaaga gacagattca
     9301 tcattgctat tctcaaagta attgggaaca ataatcccaa tgtccttatc tttcgcttca
     9361 atattggtaa actcttctaa taattttaaa gaaagattct tttgtatgat ggtatcttga
     9421 tctagtaaaa ggatataatc acatttctct tttttggcaa tttcaatacc tatgttttga
     9481 gctgtcgcaa gcccaatgtt ctcattcatt ttttttaaaa ttaagttttc catcatttca
     9541 aattgaaaag agttaatatt atctgagccg ttatccacga taataagttt gttctgtttc
     9601 tcttgtatta acgtatccac taataacttt atgttattat ctggattgta ggtaacaata
     9661 attatcccaa ttttattttt catcgttcat caccttgtac acttcattcc atttcatttt
     9721 caatacttgc tcagagaata tagtggcgta aatttttcta acattttcat ttatgttttt
     9781 attacactga taaaatttca acatctcatt ttcatcaacg gtactattat aattgattcc
     9841 gacattatat cttttaacta tttctttcgt atctcctgat aaatttgaaa ttataggtaa
     9901 tccaaaccta aaatactcaa tcgatttcag acttaaacca attatggtat ttgacttaaa
     9961 taaatttagt ccaaaatgac atttatcgag tataactttt ttttctgcat tgtcatatat
    10021 aacaccgcag tcaacaaatt caatatgatt tttttcacaa atatcagcta atttagtttt
    10081 gctttcacct tttccaataa agtaaagatt tattttgttt ccaaattttc ttttgcatgt
    10141 tttcaataat tcattaatcg cttctaaatc tacgatatta ttaatcgtac ctaaataagc
    10201 aaggttcaac gtagactcta agcttttatt gactattttc tctccataat cattttggtc
    10261 atctacattg aaagaacatg gataaatggt catatttttt gattctccgg aaatttttat
    10321 gaattttttg aaataatcgc attctgttat aactagacta gaattactga gatattgatc
    10381 tcgtagcttt ttccataaat cagtaaaaaa tttatatttg ttccctattc ccggcattga
    10441 ctctggccat aaatcataca catcaaaaat aactcgattg catctactaa acttagcaat
    10501 aaaaaaaact aaggtattag gtggtattct acaataaata atttcaggct tttcttttcg
    10561 aataaataat aatgttttta ttgaaaacca tatatgagat aaaattcgca ttacggatat
    10621 atttttttta tatccgatcg tttttatcac atgacgattt ttaatcatat gcctttcttt
    10681 actggataga aattgttttc ttatatgtga gtaatcagat acaacattaa aatattcgat
    10741 actgttttcc gtcagaattt cctctaatat attcgttcga ctgatataat caaaaggatg
    10801 aataagcagc cacttttttt tattctcact catctatctt cgcaccctca aatttctcag
    10861 tagtcccact tgctactgat gttattccat cacggacaat aactttcttt agcgttaaaa
    10921 aaaatatttt tatgtctttc atcattgaaa tgttctccac atattcaata tctttttcaa
    10981 atttttcttt ccaactaatt gcattacgac cactgacttg agctaatccg gttaaacctg
    11041 ggcgcacaaa atgtcttttt cgttgttcat tactgtatag tgaaagatac tcaactaaca
    11101 gaggacgcgg tccaataata ctcatatctc cttttaaaat attccataat tctggtagtt
    11161 catcaagact agtcgaacgc aatattttac caaattgagt caatctgata tcgtctgata
    11221 ataaattacc ttcttcatct ctttgatctg tcattgttcg aaatttatac atcagaaaaa
    11281 tcttttcatg ttttccaggt cgttgttgtt taaagatcac tggcgatcca agctttattc
    11341 gaactaggat tgcaacaaac gtcatcagtg gccataacat tattaaacct aaaattgaaa
    11401 aaagaatatc tatcaaacgt ttaaagaact tttcgtagat acctctatta gacattttgc
    11461 atcactcgct taattgtatt agttactcga tacaaatcat catcggtcat tttagaatct
    11521 gaaggtaaac agataccttt ttcaaacaat gtctgagcaa catctgtacc aatatactca
    11581 tacttatcga aaactggctg taaatgcatt ggtttccata tcgggcgggc ttctatatta
    11641 tttttatcta actcatcaat aagatgattc gtatttattc cctttactgt tatgacactt
    11701 aaccaataat ttggttcatt ccaattattt tctggcatga attgtatatt tgacaattta
    11761 gacaattctt ttttataaaa actatgaata tatcgttttt tctcaattct ttcatttaaa
    11821 actttcaatt gtcctcgacc gatacctgca acgatattgc tcattcgata attatagcca
    11881 atttcattgt gttgataatg gcgtgccttc tcacgagatt gcgtagccca aaacctcact
    11941 ttttccacct tttctttgtc attagacaca agcattccgc cacctgaagt cgtgattatc
    12001 ttatttccat taaaagaata aattccatag tcgccgaatg taccagtata ttttccttta
    12061 tatgttgttc ctaatgattc tgcagcatct tcaattaaaa caacattatg ttctttgcat
    12121 attttagtaa tttcatctag ttcagctgat aaaccgtaca aatgcactac taatacagct
    12181 tttgcgttag gatatttttc tagcgctgtt cgcaaatgtt cagggttcat attccaagtt
    12241 tcgtaatcac tatcaacaaa tactggaata gctccttgat agatgattgg attggcagta
    12301 gctgagaacg tcaaagactg acaaatcact atatcatctt taccgacccc agccgctttt
    12361 aaagctagat gtatagctgc tgttccagaa gatagtgcag ctgcatgact agcaccgaca
    12421 tattctgcta attcctcttc aaaaccattg acattcatac ccaaaggagc cacccaattt
    12481 gtatcaaatg cttcttttac atactcttgt tcatatcctt catcactcat atgaggagat
    12541 gctaataata ttcgtttatt ttccaactta atcccatctt tctatgactt tggtaggtac
    12601 gccaacattt ttagtcttat tattaatatt ttttattact acacttcccg cacctataat
    12661 gttcatattt ccaattctta gattttgtat tattttagta cctactccaa tttcattttt
    12721 atctcctaat agaacatgtc ctgaaatatt tacacttgga aaaattgaat tataattacc
    12781 gatttgagta tcatgtccga tagtagagcc gatattgatc atattgaaat ttcctaaaac
    12841 tacgtcagca gtgtaagtcg tatagggcat caaaatattg ccaattccta actgaacatt
    12901 tatgcccact agtgcacttg atgcaactaa attagggaaa atcaatttat tatttttatt
    12961 tatcttttga tatattttct ctttgatatc tggcgaagcg attcccagaa aaatagcagt
    13021 ttcttcttct aattctaata acgagtctat tgttcccaat acagtttttc ccactaattc
    13081 ttgattttgc atttttaacg aatcatcgac aaaaccaatc aattcatatc tagagaaact
    13141 ttctagtaga aaagcaatct ctttgccaaa tccaccagct cctacaataa ttgctttcac
    13201 tttactcccc ctacttattc gaagcattcg caaaagtgac aatgtcttta gccaattgtt
    13261 catcgtcttg cggcaggctc ttgacaaatt ccatcactgt ttgcatggaa taacctttga
    13321 tgtttcccac aaagatcttc tcgtaaactg cttcatcgtt tctttcctta tctaacaata
    13381 gttcttcata tagtttttcc cctggacgga tacctgtttc aatgatttcg atctcgtctt
    13441 cgctatatcc gctcaatcgg atcatgtttt ttgctagatc tacgattttc actggttcac
    13501 tcatatctaa aataaagatt tcgccgcctt ttgctaacgc cccagattga ataactaaac
    13561 gacttgcttc aggaatcgtc atgaaatatc gggtcatacg aaagtctgtt acagtgactg
    13621 gccctccttg agcaatttgt tcccggaata ctggaatcac actaccgcga gaaccaagga
    13681 cattcccaaa tctgacagct gagaatttcg tacatccttc ttcattcaac ccagtcacga
    13741 tcatttcagc gattcgtttt gttgaaccca tgacgtttgg cggattgttg gctttatctg
    13801 tcgagaccat gacgaagttt tttactttcg ctgctttggc tgcttctgct acattttttg
    13861 taccgaaaat attgtttttg actgcttctt ttgggttata ttccatcaat ggtacatgct
    13921 tatgagcggc tgcatggtag acgatatctg ggtggtattg ttccatgatc tcattgattt
    13981 tcttgcgatc ttggatatcc gcaatgatcg gcacgatttc tgtaggacac ttttgatgat
    14041 gcgtgcgtaa ttcacggtca atcaagtaaa tcgagttctc cccatgacct aaaagtaata
    14101 gttttgcagg gtcaaattgg atgatttgcc ggcagatttc tgaaccgatt gatcctcctg
    14161 caccggtgac aagaatgact ttgtttgtga tctggtcttt gatggattcg atatccaact
    14221 ctacctcatc tcttcccagt aattccacta cgtcgatggt ttttaatttt gatacgctga
    14281 ttttccctga ggcaagttct tccatagaag gcatcgtatt gactttcaca tgagcagatt
    14341 cgactaactc gaagattctt cgtaattttt ttcttgataa agaagggata gcgatcgtta
    14401 ccatttggat gtcatatttt tcgatcaatt ccggtatatc ttttaatgac cccagtactt
    14461 tttttcctga aagataggta tttcttttat tcggatcatc atccacgaat ccaatgacat
    14521 ggatatcttg agccgtttta gaaccaagaa agctgttgta taaaatacgt ccaccttcgc
    14581 cggccccaac gatcaaggta tttttagcgg aatctgttga tagatacctc atattttttg
    14641 tttcgatata gatacgccag atcaatcggc tgccaatgat cagtaatagc gaaagtaggt
    14701 atgcaaagat cactaaacgt aaactgtatt gtttatttat gaaaagcaat atgatgatac
    14761 ttgaggccgc acttgcagat aacgaaccaa aaatcgcaat gatctctctt aaatttgtat
    14821 aacgattgat tcgagtaaaa actttgaata aactaccata gaataaataa aaaccaatcg
    14881 acaaaccgat agagattaaa ataaggtcca ttggtatagc tacaaacggc ttcatgaatt
    14941 tagctgctgc aatattcgca gcaatcaaca acaagctgtc cacaaatacc aatgtggcaa
    15001 tttttcttct tcttgttaat acaaacaccc tacattcccc ctaaaataaa ccaaacttct
    15061 tcttttttac ctcttcatag actggataac tgatctcatc cccgttgatc aggtcctttg
    15121 cgacttgttt catctgtgct actttttcct taccaaagtc cttggcgatt tgctcataac
    15181 attcttttaa gtaaaaggta cgtttagaga ccccatgggc atcggaagca gccatttgca
    15241 cgaggttatg tttgaccatc attttggctg ttttctggat gtcttttcca aatacaccga
    15301 cataacttgg tgcggtgagt tgagccaagc atcccatatc taggaatggc agcaaatgat
    15361 ttgggtcttc tcgaaaatgc gcgttgcgtt ctggatggac gatcactggt gtctttccta
    15421 gttgtaacaa ctcaaaaaag agccattctg catataacgg cacttccagt gtcggaaatt
    15481 cgattagaag atatgtatca tctaaatctg taaataaaat ttcatctcgg tggatgtctt
    15541 caatcagtgt tccggtaata cgtacttctt gtccttccaa taacgtcaaa ggcaattgtc
    15601 ttttctctaa ttctgcttgt aacgaagcga ctagagaaat gacttgtgat ttttcattgc
    15661 tgtattttcc attattgtga tggggtgtgc ataaaatatg tgtgatccct tgttgaatcg
    15721 ctttttcagc catggcgata ctggcatcta tgttttctgc tccgtcatcg attcctggca
    15781 ggatatggca atgtagatcg atcatttctc ttgtctcctc tctttttatt tcttaattcc
    15841 cgtagtaata gccagcatct ctgctgtgtt ctgctccatt gtatacaaca ccaaggactc
    15901 gcgcttgtac catatctaac agttcttttg ctttagtcaa tgattctttt cgtgacgtat
    15961 tttcccgtac gaccaataag gtgccatccg tttttgaaga catgatctgt gcatctgtta
    16021 cggcaacgac tggtggcatg tcgaaaatca ctacatcaaa tagttgtctt gcttctgcta
    16081 aaatctgatc cattcttggc gaacttagta gttctgatgg atttggtggt ttaggtccac
    16141 ttggcagaat cgacaaattg tcgactggtg ttcgttggat ctcatctgct acacttcctg
    16201 atgaactaag aacagtactt aatccgcttg cgttgttcaa cttgaacgtt ttatagatga
    16261 cgggcttacg taagtctgca tcgactagca gtacccgttg accagatttg gcaaaaacaa
    16321 cggcaatgtt tgctgcagtg gtggattttc cttctccagg acctgatgaa gtcacgacaa
    16381 tcgttttgat ttgctgacca gctgcggagg caaactggat attcgtccgg atcgtacgat
    16441 attgttcggc gattggtgaa gatgggtttg ttaatgtgat caaactgact gcatgagttt
    16501 gagttgcttt ttgtttctgt gttcgtgcca tttttcttcc tcctagactc ttgagcggct
    16561 tctgcgtgat ggggctgttt tatcgtttgt agcttttggt cgtgcaggag ctgatccttt
    16621 tttacggata ccagcgttca attcttttgc tgacatttct ggaactgttc ctaagattgg
    16681 gaatcctaat gtatccgtca catatttttc atctttcact gtccgatcaa gtaattcgaa
    16741 taagaatgcc agacctacgc ctaccatcat tcctaacact aagccaatca ctagattcaa
    16801 tttattattt ggcgaaacag gtgtcgtatt ggcaactgca cctgaaataa tcgagatttt
    16861 atcgacattc atcacgtctt tggcgttttc ttggaaaact tgtgaagttg tattagcgat
    16921 ctgttgagca gtcactgcat tcgtgcttat tgcctggatc gagaacattt gtgaattttg
    16981 tgactgattg acggagatcg catctttgat ttgtccagca gtcattttca cattgtcttc
    17041 tgtttctaaa cgatctttga cttcatttag tactaaatca ctgacgatca tatctttata
    17101 cgtattgatc atttgcaggt tcgtattgac atcattgaca tttgctgtgt ctgattgtgg
    17161 cagtgtcacg atcaattgtg cttgtgaact gtattttggt gtcaagatga aaaatgttac
    17221 gattcctgag atggccaagc cagcaaacat tgagatgatg attgttacca agtgtttgcg
    17281 taatgtttca aaaatttctt tcaagctgat tgtctcttcc attttttctc cactttccta
    17341 tttattctct agattcattt gatcttttaa ttcttttttc acacgatcaa gttcttgatc
    17401 gctgactttt tgataggaga tattatcttg catgaagcct tctccttgca gctgatcttg
    17461 cttgatcgta ccaaatgcct cccgataatc taaagcgatt tttttcattt catcaaagct
    17521 catgtctgtt ttcatattcg actctagagc attcaaaacg gattgatagt tgctgacacc
    17581 gtttaaactc aatacttttt ttgctacacc ttctacgatc tttctttgac gctcttgtct
    17641 accatagtca ccatttggat cttcataacg catacgagaa taagataatg cctgatcccc
    17701 gttcaatgta attttaccga ttggaaagtc atagccatct tgtgagaaag tgaggtcatt
    17761 attgacttcg ataccaccaa ctgcatcaac gagttccttt agccctttca tattgataga
    17821 gacatagtga tcgattggga tgtctacata tttttgtagg gtatccatcg ccatggcagc
    17881 tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac
    17941 atacgtatct cgaggaatgc tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc
    18001 aaccatcatc gtatctgatc ttccttgatc gacccgacct aaatctccag tatcaatgcc
    18061 catcaataga acagagaaag attcttgttt gtccagatca actggtttcg ttcgttctcc
    18121 ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa tgtgagagat ccagatataa
    18181 ttttgctcct actcctacaa ttgctaatac taatacgata agaactgcta aaataatcag
    18241 aaatattttt ttccctcttg attttcttct tcgtcttgcc atagtttcct ccgtttgcta
    18301 accttgcttg aatcaacgtt ttaactgtca ataacttata ttgctatagc ccatttgctg
    18361 agcaacagtt tgtgcttttg ttgcgtatgc tgcgcttatt ttttcattcc ggatctgctc
    18421 tatttgactg agcgccgtca aatattgatc gtaagtagcg gtggcagtca cttgaccatc
    18481 cttcaacatc gtatcgaata actgatctag ttgcgtgact tgcgcgatct gagtatcagc
    18541 gaatcctaga tattgcataa caaccgtttt ccaaggacta tctttaaaaa agctcatatt
    18601 ttcacgtaca cgagcgacat ctgcacttgt caccttttct ttgatgacga catcgtctac
    18661 agcttgctgc cagtttgata catttttagt aaataaatcg ttgacagctg tctggatctt
    18721 cagttttgat tctgcactat ctaactgtgt cacgattgct ttcttctcat cttggattgc
    18781 aacagcttct ttaggcaatt ctgattcttt gatgtcgtaa tcatctgctg aaagtttgac
    18841 agaggatagc ttatttgaca aacttgtgat tgtttcttct gttatttcag ggtttagaaa
    18901 atttttttct tttgtactat acagcttgtc tacttctttt gataaagaag tcaaggtgtc
    18961 tttttgattg gtgatggcag attctgcata attgatccgt tgctgatgtt gccgctgatt
    19021 attgatatgg taaccaaaca aacagatcgc aatagcacct gcacctaccg ccacagcagt
    19081 caatgacttg acgaatggct tttccataga ttcactccta agtattgatg atagataaat
    19141 taagatataa ttcacataca taaaaatgac agctttttat tttaaaaaac ataattaaat
    19201 taatatattc acagaaatat ttgtaaacaa gttttcccta tataatcata ccacaaattg
    19261 ttttcttgac tagggcaatt tacgatttaa aagtaaataa aacattaata agataacaat
    19321 taatcgattt catttttatt tccttaattt tcataggtgt ttttaatttt
//
LOCUS       DANJMA010000048        18123 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000048 DANJMA010000000
VERSION     DANJMA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(24..722)
                     /locus_tag="PWU31_002038"
     CDS             complement(24..722)
                     /locus_tag="PWU31_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL1084538.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="PWU31_002039"
     CDS             complement(715..2067)
                     /locus_tag="PWU31_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL1084539.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="PWU31_002040"
     CDS             complement(2290..3690)
                     /locus_tag="PWU31_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL1084540.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="PWU31_002041"
     CDS             3851..4588
                     /locus_tag="PWU31_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL1084541.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="PWU31_002042"
     CDS             4713..5192
                     /locus_tag="PWU31_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL1084542.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="PWU31_002043"
     CDS             5189..5956
                     /locus_tag="PWU31_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084543.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="PWU31_002044"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="PWU31_002044"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1084544.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="PWU31_002045"
     CDS             complement(6859..7461)
                     /locus_tag="PWU31_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1084545.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="PWU31_002046"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="PWU31_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="PWU31_002047"
     CDS             8142..8726
                     /locus_tag="PWU31_002047"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL1084546.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="PWU31_002048"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="PWU31_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL1084547.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="PWU31_002049"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="PWU31_002049"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL1084548.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="PWU31_002050"
     CDS             10627..10806
                     /locus_tag="PWU31_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084549.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="PWU31_002051"
     CDS             10828..11853
                     /locus_tag="PWU31_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL1084550.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="PWU31_002052"
     CDS             12028..13272
                     /locus_tag="PWU31_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL1084551.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="PWU31_002053"
     CDS             13425..14372
                     /locus_tag="PWU31_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084552.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="PWU31_002054"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="PWU31_002054"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL1084553.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="PWU31_002055"
     CDS             complement(15274..15516)
                     /locus_tag="PWU31_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084554.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="PWU31_002056"
     tRNA            15637..15709
                     /locus_tag="PWU31_002056"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="PWU31_002057"
     CDS             complement(15828..16466)
                     /locus_tag="PWU31_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1084555.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="PWU31_002058"
     CDS             complement(16589..17221)
                     /locus_tag="PWU31_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL1084556.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="PWU31_002059"
     CDS             17572..17871
                     /locus_tag="PWU31_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1084557.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6213 a         3162 c         3570 g         5178 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtctggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tat
//
LOCUS       DANJMA010000049        18106 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000049 DANJMA010000000
VERSION     DANJMA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(164..388)
                     /locus_tag="PWU31_002060"
     CDS             complement(164..388)
                     /locus_tag="PWU31_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL1084558.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="PWU31_002061"
     CDS             complement(586..2166)
                     /locus_tag="PWU31_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL1084559.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="PWU31_002062"
     CDS             complement(2567..3943)
                     /locus_tag="PWU31_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1084560.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="PWU31_002063"
     CDS             complement(4071..5189)
                     /locus_tag="PWU31_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1084561.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="PWU31_002064"
     CDS             5331..5765
                     /locus_tag="PWU31_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1084562.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="PWU31_002065"
     CDS             complement(5843..6376)
                     /locus_tag="PWU31_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL1084563.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="PWU31_002066"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="PWU31_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL1084564.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="PWU31_002067"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="PWU31_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL1084565.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="PWU31_002068"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="PWU31_002068"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1084566.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="PWU31_002069"
     CDS             complement(10101..10679)
                     /locus_tag="PWU31_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL1084567.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="PWU31_002070"
     CDS             11001..11471
                     /locus_tag="PWU31_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL1084568.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="PWU31_002071"
     CDS             complement(11579..12805)
                     /locus_tag="PWU31_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1084569.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="PWU31_002072"
     CDS             complement(13559..13759)
                     /locus_tag="PWU31_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1084570.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="PWU31_002073"
     CDS             complement(13893..14501)
                     /locus_tag="PWU31_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL1084571.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="PWU31_002074"
     CDS             complement(14586..15056)
                     /locus_tag="PWU31_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL1084572.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="PWU31_002075"
     CDS             complement(15104..16018)
                     /locus_tag="PWU31_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1084573.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="PWU31_002076"
     CDS             16164..16967
                     /locus_tag="PWU31_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL1084574.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="PWU31_002077"
     CDS             17145..18092
                     /locus_tag="PWU31_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL1084575.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5437 a         3631 c         2964 g         6074 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaat
//
LOCUS       DANJMA010000050        17789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000050 DANJMA010000000
VERSION     DANJMA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            78..890
                     /locus_tag="PWU31_002078"
     CDS             78..890
                     /locus_tag="PWU31_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL1084576.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            887..1687
                     /locus_tag="PWU31_002079"
     CDS             887..1687
                     /locus_tag="PWU31_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL1084577.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1848..3116
                     /gene="rseP"
                     /locus_tag="PWU31_002080"
     CDS             1848..3116
                     /gene="rseP"
                     /locus_tag="PWU31_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL1084578.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3184..4893
                     /locus_tag="PWU31_002081"
     CDS             3184..4893
                     /locus_tag="PWU31_002081"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL1084579.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5105..9457
                     /locus_tag="PWU31_002082"
     CDS             5105..9457
                     /locus_tag="PWU31_002082"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL1084580.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9601..10074
                     /gene="rimP"
                     /locus_tag="PWU31_002083"
     CDS             9601..10074
                     /gene="rimP"
                     /locus_tag="PWU31_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL1084581.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10098..11273
                     /gene="nusA"
                     /locus_tag="PWU31_002084"
     CDS             10098..11273
                     /gene="nusA"
                     /locus_tag="PWU31_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL1084582.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11295..11588
                     /locus_tag="PWU31_002085"
     CDS             11295..11588
                     /locus_tag="PWU31_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL1084583.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11585..11896
                     /locus_tag="PWU31_002086"
     CDS             11585..11896
                     /locus_tag="PWU31_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL1084584.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11909..14215
                     /gene="infB"
                     /locus_tag="PWU31_002087"
     CDS             11909..14215
                     /gene="infB"
                     /locus_tag="PWU31_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL1084585.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14242..14589
                     /gene="rbfA"
                     /locus_tag="PWU31_002088"
     CDS             14242..14589
                     /gene="rbfA"
                     /locus_tag="PWU31_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL1084586.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14697..14918)
                     /locus_tag="PWU31_002089"
     CDS             complement(14697..14918)
                     /locus_tag="PWU31_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084587.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15366..17729
                     /locus_tag="PWU31_002090"
     CDS             15366..17729
                     /locus_tag="PWU31_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDL1084588.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6068 a         3152 c         3917 g         4652 t
ORIGIN      
        1 atttcatagg aaaaacagtt agatattgct ataatactta agatagtggg aatcttttaa
       61 gtataggagg aaaaagaatg ttacgttttt ttccgcaaaa aaacaaatat attcaagaaa
      121 aaagcgaatt tcaatttaat accgatggac caattcctca gcacattgcc atcatcatgg
      181 acggcaatgg tcgttgggcg caaaaccgtc gtttgccacg agttgctggc cataaagaag
      241 gcatggaaac agtaaagaaa gtcacaaaaa aagcatcaag gcttggagtg aaagtgttga
      301 cgctttatgc cttttcgact gaaaactgga agcgaccaaa agatgaagta agttttctca
      361 tgcaactacc tgtcgacttc tttgatacgt tcgtaccaga attgattaaa gaaaatgtca
      421 aagttcatgt catgggttat gaaaacgtat tgccagaaca tacgcaagat gctgtaagac
      481 gtgcaattga gcaaacaaaa aacaatactg gaatggtatt gaattttgct ttgaattatg
      541 gaagtagagc agagatcgtg actgctgtcc aagaaattgc cgaagaagta gcaaaaggag
      601 aaatacacgc agaagaaatc gatgatgaat tgattgctaa gcatttaatg acaggtttct
      661 tgcctaaaga gttacaagat cctgagctga tgatccgtac aagtggagaa gagcgaatta
      721 gtaattttct gttatggcag atcgcatata gtgagctgta ttttacaaaa gcgctttggc
      781 ctgattttga tggtgcgcat cttgaagaag caatcgcctc ttatcaaaat cgagaccgtc
      841 gattcggcgg tgtaaaaaaa acagaagatg agaaagggga tcagtcatga aacaacgtgt
      901 gatcacagca gctgtggcgc tagtcttgtt tattccgatt atatggatcg gaggcatagc
      961 ggttgagctt acagcagcaa tcttagcggt agtaggagtt tacgagctgt tccggatgaa
     1021 aggattgaca ctcttaagtt ttgaaggagt tttatccgcc gttggtgcag tgttacttgt
     1081 tttgccaaaa gaaagatggt ttttctttct tccagagaat gtaagtacat ttatgttatt
     1141 ttatcttata gttatgattc ttttaggaac atcagttatt tctaaaaata cgtatacgat
     1201 tgatgaagca ggttttcctg tgcttgtcag cttgtatgtg ggaataggtt tccagaattt
     1261 tgtcaatgca cgaacagaaa gcttggctgt tttgatattt ggtctattta ttgtttgggc
     1321 aactgatatc ggagcatata tggttggtcg acgttatggt cagcataagt taagtcctga
     1381 tatttcgccc aacaaaacga ttgaaggtgc gctcggtggt atcggtagtg ctgttgtagt
     1441 cgcatttctt tatttattgc tatacccagc aaaagaacta ttcaatcatg gtatgtttgt
     1501 catgcttctg atgacgatta ttttttcagc agtcggacaa tttggtgatt tggtcgaatc
     1561 tgcaatcaaa cgtcattatg atgtgaaaga ctcgggaaat atcttaccag gccatggtgg
     1621 aatcttagac cgtttcgaca gtttgctgtt tgtatttcct atcatgcatt tatttggcat
     1681 tatttagtaa gtatacctgc gtttctttgt gaatttcata aaagacgcag gtttttattt
     1741 ttcattatat tgatactatt ggtgtctttg gtatactatg gtaaaattat agtgttttat
     1801 cgttgtttta taaaaaacac aaaaaacaag taaaggagaa aaaagctatg aaaacgattc
     1861 tgacatttat catcgttttt ggtatattag tgattgttca tgagtttggt catttcttct
     1921 ttgcgaaacg atcaggaatc ctcgtccgcg aatttgctat cggtatgggt ccgaaaattt
     1981 atgggcatca agcaaaagac gggacaacat atacgcttcg cctattacct attggcggtt
     2041 atgttcgaat ggcaggcaat ggggatgatg aaacagaaat ggcaccagga atgcctcttt
     2101 ctcttttact aaattctgac ggcatcgtag agaagataaa tttaagtaag aaaatccaat
     2161 tgacaaatgc tatcccgatg gaattgagcc gttatgattt agaagatgag ctgactatca
     2221 ctggttatgt gaatggagac gaaacagaag tagtaaccta ccctgttgat cacgatgcga
     2281 cgattattga aaacgacgga acagaaatca gaattgcgcc aaaagacgta cagttccaat
     2341 cggctaaatt gtggcagcgt atgctgacca actttgctgg acccatgaat aactttattt
     2401 tggcaatcgt gttattcatc attctggcat ttatgcaagg tggtgttcaa gtgacgaaca
     2461 ctaatcgcgt aggagagatc atgcctaatg gagctgcggc agaagcaggc ttaaaagaaa
     2521 atgatgaggt agtcagtgtg gacggaaaag aaatccattc atggaatgat ctgacaacag
     2581 tcattacgaa aaatccaggt aaaacattag actttaagat cgaacgagaa ggtcaggtgc
     2641 aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg cgaaaaagtt ggccaacttg
     2701 gtataaaagc cccaatgaat acaggtttta tggataagat cattggaggg acgcgtcaag
     2761 ctttcagcgg ttcattggaa attttcaaag ccttaggatc actatttaca ggtttcagtt
     2821 tagataaatt aggcggacct gtcatgatgt accaattgtc ttcagaagca gcaaatcaag
     2881 gtgtaacaac tgttattagt ttgatggcgc ttctttcaat gaatctcgga atcgtcaatc
     2941 tgcttccgat tcctgcctta gatggcggga aattagtctt gaatattttt gaagggattc
     3001 gcgggaaacc attaagtcaa gaaaaagaag gaatattgac attagctggc tttggatttt
     3061 taatgttgtt gatggtgcta gttacatgga acgatattca acgattcttt ttctagaaaa
     3121 agttgacaaa tagggtataa taacaaacga taactatttt gaggaaataa aggagtatat
     3181 agaatgagac aatcaaaaat gttgattcca acattacgcg aagttccaaa tgatgcagaa
     3241 gtattaagcc atcaaatctt gctaagagct ggatatatcc gccaagtatc agcgggtatt
     3301 tattcatatc tgccattagc aaatcgtgta ttggaaaaac tgaaacgcat tatgcgggaa
     3361 gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg ccatcttgcc agcagaactt
     3421 tgggaagaat ccgggcgtta cgaaacatat ggaccaaact tgtatcgttt caaagaccgc
     3481 aatgatcgaa aaatgatcct tggaccaact cacgaagaaa cttttactga tttgatgcgt
     3541 aatgaaatca attcatataa gaaattacct ttaaatcttt atcaaatcca acctaaatat
     3601 cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa gagagtttat catgaaagat
     3661 gcatattctt tccatgcaac ggaagaaagt ttggacgaga catataaaga ttacgaaaaa
     3721 gcctataccg aagtattcaa gcgttgtggc ttagaatttc gctcgatcat cggagacggc
     3781 ggcgcaatgg gcgggaaaga ctcaaaggaa ttcatggcga tctcagaaat cggggaggat
     3841 acgatctgtt attcgactga aagtgattat gctgcaaact tggagatggc tacaagctac
     3901 tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg agaaagtagc tacacctaac
     3961 gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg aacctcaaaa aatcatcaag
     4021 tctgtattgt tcattgcgga tgaacaacct gtccttgttt tggtacgtgg agaccatgaa
     4081 gtcaatgacg taaaactgaa aaacttttta tctgctgact ttttagaaga agcaacagaa
     4141 gaagaagcac aaaaatatct aggtgctgag tttggttctg tcggaccagt cggtgtttca
     4201 gatgacgtaa aagtatatgc agaccgttat gtccaagatt tagcgaatgc cattactgga
     4261 gcgaacgaaa caggattcca ctataaaaat gtgaatccaa aacgcgattt cactgtgctt
     4321 tcttatgaag atcttcgttt tgtacaagaa ggagatcctt caccagacaa caacggtgtt
     4381 cttgccttta ctcgaggaat cgaaatcgga catatattca aaatcggcac tcgttacagt
     4441 gaatctatgg gagcaactgt actagacgaa aatggacgag aaaaatttgt gattatgggc
     4501 tgttatggta tcggtgtaag tcgtttatta tcagcaatcg tagaacaaca ttcagatgaa
     4561 caaggaatca attggccaaa aggaattgcg ccatttgatc tgcatatcgt ccaaatgaat
     4621 ctaaaagacg aataccaaac aagcctgacg aatgaactag aagaagcgat gacaaaagct
     4681 ggctatgaag tactggttga tgacagaaat gaacgcgcag gagtgaaatt cgcggattct
     4741 gatttgattg gctgtccaat cagaatcact gtcggtaaaa aagcagtgga tggaatcgta
     4801 gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac gtaaagaaga attggcgaat
     4861 actttaccaa tcctcttgaa ccaaggcgaa taataagcgt taaaacacag aaaataaaaa
     4921 tagctgaatc agctgttttt gacaatctga tgattcgctt ttaaaattgg aactgcgatt
     4981 tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa aacgagtata ctaaaaggta
     5041 ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt tctcatcaga aagggcgtat
     5101 atctttgtca gaaaaacgag atttatttga aaaactgttg gaacaaatcc aactagagga
     5161 agcagaaaaa aatcatccac tgcttactgc tggtgaaatg gatagtgtag tggtccatcg
     5221 taaatcgcgt ttatgggaat ttacgctaca tttttccaaa attttgccta ttatgctata
     5281 tcggtcattg actcagcatc tgactatcgc ttttaaagat attgcacaag tcaaactgaa
     5341 tatcttagca gatgaccaga cattcgatga acagctcttg caagattact gggcccaagc
     5401 tttggaaaat cagcaatgcg atacaccact agcacagcaa gtattgaaaa cgcaggtacc
     5461 tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt acaggagcta tttcttattt
     5521 gaagcaacaa tacttgccat tagtagaaga gttgttcgtc agttacggat tcccaaaatt
     5581 tcgtatcgaa ccagaagtgg atgagcagca agcggaacgt gtattgaaac tttttgaaga
     5641 acgcaaacaa gaacaggcag aagcctttat gaagcaagca gctgaaagtc tagttgtcca
     5701 cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct cttgaaggac ccatccagct
     5761 aggacgaaat attccagccg atgaaccaac gacaccaatg atcagtatcg ttgaagaaga
     5821 acgaagagtc acacttgagg ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa
     5881 acgcaagatc ttgactttga aaatcacaga ttatacttca tcatttatcg tcaaaaaatt
     5941 ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta gctggttaaa
     6001 ggtacgagga agtattcaag aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga
     6061 tatcatagaa gtcaaacata cacctcgcaa agattatgct cctgaagggg aaaaacgagt
     6121 agagcttcat gttcatagca atatgagtac gatggatgca acaaacagca tctctgatct
     6181 tgtggcacaa gcaggaaaat ggggacaccg agctatcgcc attacagacc atggtggtgc
     6241 gcaagccttt ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg
     6301 cgtggaagcg aatgtcgtag atgatggtgt cccaattgct tataatgatg cacatgaagc
     6361 attgagtgaa gccacatacg tcgtatttga cgtggaaacg acaggattgt ctgcagtata
     6421 tgacacgatc atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt
     6481 tgatgaattt atcgatccag gacatccatt gtcacgaaca acggtagatc tgactggtat
     6541 cacggatgaa atggttcgag ggtcaaagtc ggaagaagaa gtattgcgga tgttccttga
     6601 attttcaaaa gatacaattt tggtagccca caacgcggct tttgatatgg ggttcttgaa
     6661 tactagctac gcaaggtatg gtattcctga agcggccaat ccagtcattg atacgttgga
     6721 attagctaga tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa
     6781 gtttggtgtt agcttggaac agcatcaccg agcaatctat gatgctgaag caactggtca
     6841 cttagcatgg atctttgtga aagaggcaat ggacaatcat aacatgcttt atcatgatca
     6901 gctaaatgag catatcggag aaggagattc ttacaaaagg gctcgacctt tccatgtaac
     6961 gattttagca aaaaatcagg caggactgaa agatttgttc aaactgattt caatgtcgaa
     7021 tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc caattgaaaa agatgcgtga
     7081 aaatctattg atcggctcag cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa
     7141 aggggtagaa gaggcaagaa accgagcaaa attctatgat tatattgaag ttatgccaaa
     7201 agcggtctat gcgcctttga tcgagcaaga attggtaaaa aatgaacatg atttggaaga
     7261 aattatccaa aatttggtag aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg
     7321 taatgtacat tatctgaacg aagaagatgc catttaccga aaaattttga tcaattctat
     7381 gggcggagcg aatccgttga atcgtcatag tttgccggat gtccatttcc gaacaaccga
     7441 tgagatgcta actgcatttc actttttagg ggaagaaaca gcgaaagaga tcgtagtaga
     7501 aaatacgaat aaaatcgcag atatctgcga agaagtgatt ccagtcaaag atgaacttta
     7561 tacaccgaag attccaggtt cagaagatga aatatcagaa ttgagttaca caaaagcaaa
     7621 acaaatgtac ggagatccgt tacctgagat cattcaaaaa cgattagaaa aagagttgaa
     7681 ttctattaat ggtaatggct tctcggttat ttatctgatc gcccaaaaac tagtgcataa
     7741 aagtaatgaa gatggctatc tagtaggttc acgtggctct gttggctcta gctttgttgc
     7801 gacgatgaca ggaatcactg aagttaatcc attagcccct cattattatt gtccagagtg
     7861 ccaatattcc gagttttttg aagatggtac gtatggttct ggatttgaca tgccagaaaa
     7921 acaatgtcca aaatgtggtg cacgcttaaa caaagacggt catgatattc catttgagac
     7981 atttttagga ttccatggag acaaagtgcc cgatattgat ttgaacttct caggtgatta
     8041 tcaagcagaa gcccataact atacgaaagt attgtttggc gaagactatg tttaccgtgc
     8101 cggaacgatt ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag gatacgaaag
     8161 ggacaataat ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg
     8221 agtcaaacgg acaaccggcc agcatccagg ggggatcatc gttattcccg attatatgga
     8281 tgtctatgat tttacgccga tccaatatcc agcagacgat caaaattcag aatggaaaac
     8341 gactcatttt gatttccact cgattcacga taatgtattg aaattagata ttctagggca
     8401 tgatgaccca actgttattc ggatgcttca agatttatca gggatcgacc cacaaacgat
     8461 ccctacggat gacccggaag tgatgcggat atttgccggt cctgaagtac ttggagtaag
     8521 tcaggaacaa atttattcca aaacagggac attaggtatt ccagaattcg gcactcgttt
     8581 tgttcgaggg atgttagaag aaacgcatcc tacaactttt gcagaactac tgcaaatttc
     8641 cggactatct catggtacag acgtatggtt aggaaatgcg gaagaattga ttcgacgagg
     8701 agatgcgaca ctggcggaag taatcggctg tcgtgatgat attatggttt atttgatcca
     8761 tgctggatta gatagcggga tggcatttaa aatcatggaa actgttcgta aaggacaatg
     8821 gaacaagatc cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt
     8881 gcccgactgg tatatcgatt cttgttcaaa aatcaaatat atgtttccga aagcccatgc
     8941 tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt tccctatttt
     9001 gtattactgt gcatactttt ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa
     9061 aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg gacaagggat tagatgcttc
     9121 cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc aatgaaatgc tggaacgagg
     9181 attcaaattt ggaatgattg atttgtataa atcggatgcc gttaactttg ttattgaagg
     9241 tgatacgttg atcgcaccat ttagagctgt cccaagtttg ggaaccaacg tggcgaaaca
     9301 aatcgttgaa gcaagaaaag acggtccatt tttatccaaa gaagatcttg ccacccgcgg
     9361 taaagtttca aaaacattga tcgaatatat gaacgacaat ggcgtattga aagatctacc
     9421 agatgaaaat caattatcgt tattcgatat gctataagca tctgttttcc tgtctgcttg
     9481 taattttaag cagagtatgg tatagttctt ataggtaatg atactaactg tgagtgagcg
     9541 gatttttcgc tcactctttt taatggaatt acgcacttaa tttttaagga ggcgaaactt
     9601 ttgagtagcg tcgttgaaac agtcacagaa atggtgacac cgattttaga tgaacaaaat
     9661 tttgaacttg tcgaagtcga atttgtaaaa gaaggaaaaa gctggttctt acgagtattt
     9721 attgacaaag aaggcggtat cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt
     9781 gaaaaacttg atgcagcaga tccagatccg attcctcaag cttatttcct ggaggtatcc
     9841 tccccgggag cagagcggcc attgaaaaaa gaaagcgact atgaaaaggc acttggtgat
     9901 tatgtccatg tctccttata ccaaccagta gatggcgaaa aacaatatga aggatttctt
     9961 caatcatttg atgctgaaca gcttacattg aaaatacgca taaaaacacg agaaaaagaa
    10021 ttgacttttg accgaaaaaa tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg
    10081 agaggaatca gaaaaaaatg agtaaagaaa tgttaaatgc gttagacgct ttagaagctg
    10141 aaaaagggat ctcaaaagaa atcgtgatcg atgctttaga agcagcattg gtttctgcat
    10201 acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa
    10261 agatccatgt ctatgcggta aaagaagtaa cggaagaagt gatggattca cagttggaag
    10321 tctcattgaa agatgcactt ttaatcaatc cagcatatga gatcggtgat acgatccgtt
    10381 ttgaagtaac accaaaagat tttggacgga tcgctgcgca aacggctaaa caagttattt
    10441 tacagcgtgt acgtgaagca gaaagaacga ttatttataa cgaattcagt gcttatgaaa
    10501 aagacatcat gcaaggaatc gtcgaacgcc aggataaacg ttatatttat gtcaatcttg
    10561 gtaaaattga ggcagttctt tctaaacaag atcaaatgcc aaatgaattt tatcaaccac
    10621 atgatcgaat caaagtttat gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat
    10681 ttgttagccg cagccatcca gacctattgc gtcgcttatt tgaacaagaa gtaccagaag
    10741 tctatgatgg aatcgttgaa attgtcagca tcgcccgtga agctggcgat cgttcaaaag
    10801 tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg
    10861 gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga aaacatggat atcgtcgaat
    10921 ggaatgaaga tccagctgta ttcatcgcaa atgcattgaa tcctgcacaa gtcatggatg
    10981 tgatcttcga tgagacaaat ccaaaggcct gcacagttgt cgttccagac taccaattat
    11041 cgctagcaat cggtaaacgc ggacaaaatg cacgtttagc tgctaaattg actaaccata
    11101 aaatcgatat caaatcagaa tctgacatga cggaatttta tgaaaatcaa gaacaacaaa
    11161 aagaaacaga agaactgcat gatgaagcaa tcgttcagtc agatttaaca gacgatgaat
    11221 atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa accagaagct taactttagt
    11281 aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg
    11341 aaatgaaacc taaaaaagag atggtccgaa tcacccgttc aaaagaaggg gaagtatcga
    11401 tcgatccaac tggaaaaatg cctggacgag gagcgtatgt ctcacttgag ccggaagaag
    11461 tacaacaagc ttgggataaa aaaattctcg atcgagtact cgaaacaaca ttaacagatg
    11521 agttttatca agaacttctt gactatgtca ctcaccaaaa agcacgtaaa gagttatttg
    11581 gaaaatgaat acagttaatc ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc
    11641 gggaaaacta atcacgggtg aagaactgac cattgctgaa atccgcaaac aaaaagcaaa
    11701 attggtgttt gtggcaaccg atgcaagtga gaatacaaga aaaaagataa aagataagag
    11761 ttcatattat gaagttcctt gctttgagct attttctgaa gcagaaatca cgcagatgat
    11821 cggaaaacct cgcaaggtat tcggaataat ggatgccgga tttgcgaaga agaccaagga
    11881 gctaattgaa ggttaggaag gtgattgcat gggcaacaaa agaatctatg aattagcaaa
    11941 agaactcaaa aaatctagta aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt
    12001 gaaaaaccat atgggtgcga ttactagcag tgatgagaag aaattgcaac aagcgttcaa
    12061 aaacccaagc acgaaacaac aagctgcaca acaagcaagc agaaaaccag ccaatcaaca
    12121 gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata aaaataaccg
    12181 cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac
    12241 aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt caaggaacga gccaaggcgg
    12301 caatcaaaac cgtactaaca ataacaacaa accaaacaat aacaatcgcg gcaaatttaa
    12361 taataataac cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac
    12421 taaaccagct gtaccaccac gcaaattccg tgaattgcca gatgtattag agtatactga
    12481 aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa
    12541 aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa gcactggata aagatacgat
    12601 tgaactgtta gcgacggact atggcatgga accacaagaa aaagtccaag ttgacattgc
    12661 tgatatcgat aaattcttcg aggcagaaga agtcgatcca gataaattag tctcacgtcc
    12721 gccagtagta acgattatgg gacacgtaga ccacgggaaa acaaccttat tggatacatt
    12781 gcgccattca cgtgtaacaa gcggcgaagc cggcggaatc acacagcata tcggtgccta
    12841 ccagatcgat attgatggta aaccaatcac tttcttggat acaccaggac atgcggcctt
    12901 tacaagtatg cgtgcacgtg gagcaagtat cacagatatc acgatcttag ttgttgctgc
    12961 agatgatggc gtcatgccac aaacagttga agcaatcaac catgccaaag ctgcaggcgt
    13021 tccaatcatc gtcgctgtaa ataaaattga taaaccaggt gcaaatcctc agcatgttat
    13081 gcaagaatta agtgagtatg aattaattcc tgaagcatgg ggtggagaaa cgatttttgt
    13141 tgagatctca gcgaaattcg gccaaaacat cgaagaattg ctggaaatga tcctgttagt
    13201 cgcagaagta gaagatttaa aagcagatcc aacacaacgc gcaatcggaa cagtgatcga
    13261 agcacgtctt gataaaggta aaggtcctgt gtcaacctta cttgtccaag aaggaacact
    13321 tcacgttggt gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg taatgacaaa
    13381 cgatcttggc cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa tcactggctt
    13441 gaatgatgta cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa agacagcacg
    13501 tgcagcgggt gaagaacgcg caaaacgtgc attattggaa aaccgtgctg ccacaagccg
    13561 tgtgactttg gacaacttat ttgaaagcct gaaagaaggc gaattgaaag aagttaacgt
    13621 aatcatcaaa gcagatgtac aaggttcagc agaagcactg gctgcatcat tgaagaagat
    13681 tgatgtggaa ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa tcaatgaaag
    13741 tgatgtaacc ttagcagcag ccagcaacgc tattatcatc ggtttcaacg ttcgtcctac
    13801 accgcaggca aaagtgcaag ctgatactga agaagtcgat attcgtcttc accgtatcat
    13861 ctataaagcc atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc ctgaatttga
    13921 agaaaaaatc accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta aagttggtac
    13981 gattgcaggt gcctatgtaa cagaaggata tattcgtaga gatagcggtg tccgtgtaat
    14041 ccgtgacgga atcgtaatct atgaaggcca acttgcaagt ctgaaacgat tcaaagatga
    14101 cgtgaaagaa gtcaaaatgg gttatgaatg tggtgcgatg attgaaaaat tcaatgacat
    14161 caaagtagat gacgtaattg aaggatttat catggaagaa gtcaaaaatg actaaaaact
    14221 gataaaagga ggccaatcat tatggctaac taccgtgatc gccgagtcgg tcaagaaatc
    14281 ttaaaagaag tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt ggagaacgta
    14341 acgatcacag atgtgaaagt tacgggggac ttacagcaag caacgatcta ttacagcatt
    14401 ttatctgact tagcatcaga taagaaaaaa gcacaagatg gattgaataa agcatcagga
    14461 ttgattcgtc gagagttagg gcacaatcta agtatttata agacacctga attgttcttt
    14521 gaacttgatg aatcagttgt ctatgggaac catatcgatg aattgcttcg cgacttgaac
    14581 aaagactaat caaaaaatcc cgcaaatcct gtgaaatcag gggttgcggg atttttttgt
    14641 tttcattaag acgtattacc taagagttcc atgactttag tctggtagcg tgtcagctaa
    14701 ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctata gtatataaat
    14761 ttttgcatat agataaagat caagataccg ataacggaag ctagattgta atctatttta
    14821 gtgacagtca ggtatagcca agtaactaga agatcagtgg aacctgcacc ataaaagttt
    14881 gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt tgaagttatt gatatttccg
    14941 attacgccgc ttcgatttgg tcacgatcca aattttggat aatccctgta gaaattagca
    15001 tagtagctgg gataccaatt cacatattga caaagataac agagactttt acataaaacg
    15061 gatcaatcag gaaaggaatt gctcgatcca gccccagttt tccaacaatg tgttaactgg
    15121 cctagcgtca ttcagcaaat tatgcatgat gaggagcgaa atgaactgcg gaacagccat
    15181 tgtgataacg acaagttttt tggatcaatg taaaaaacgt cagcgaaagg aaaaatttcg
    15241 tggtttttta tacctgttca tttggctaga tataaaatcc aaacaaatga acagaagtgc
    15301 ttattaggtt tcaattggga gtatctgcgc tagaataaga gtaataatga aaaggagtgt
    15361 tatgtatgga ttattctagt aaagaatact ttgataaaat gacggcttgg tggcgtgctg
    15421 ctaattatct ttctgttggg caactttatc ttaaagataa ccccttactg agacgtacac
    15481 tgaaaccaga agatgtcaaa aaacatccaa tcggtcattg gggaacgatt cctggccaaa
    15541 attttattta tgtccatttg aaccgagtca tcaataaata tgatttgaac atgttttata
    15601 tcgaagggcc aggacacggc ggacaagtca tggtatcgaa tgcttattta gatggaagtt
    15661 acacagagat ctatccagaa gtaacggaag atgagacagg aatgcaaaaa ttattcaagc
    15721 gattttcatt tcctggaggc attgcttctc atgcagcacc agaaacacca ggttctatcc
    15781 atgaaggcgg agagcttgga tactctcttt cacatgcagt cggagctgta ttagacaatc
    15841 ctgaagtaat ctctgctgtc gtgattggtg atggtgaagc agaaaccggt cctttagctg
    15901 gaagctggtt ctcaaatgtc tttatcaatc cagtgataga tggagctgtt ttaccgatcc
    15961 ttcatttaaa tggtgcaaaa atagccaacc caacgatctt ggcacgtaaa tcagatggag
    16021 aattagcaaa ttactttaac ggacttggct gggagccttt ctttattgaa ggaaatgatc
    16081 cagaaaaatt aaatcctgtt atggctgaaa aaatggatca agcaatcgag aaaatcaaat
    16141 ccatccaaaa agaagctcgc ctgaagacag caacagatgt cgtgatgcca aaatggccag
    16201 tattgatcgt acgcacacca aaaggatgga ccggtccaaa agaatgggac ggggaaccaa
    16261 ttgaaggaac cttccgagca caccaagtac cgattcctgt agatcaagaa catatggatc
    16321 atgcagatgc tttactccgt tggctgaaat cctatgaacc agaaaaatta tttgatgcac
    16381 aaggacgtat tttagaagaa atcagagaaa ttgcgcctac tggtgaccaa agaatggcaa
    16441 aaaatcctat caccaatggt ggaatcgatc caagaccgct tattatgcca gattggaaaa
    16501 aatatacttt gcaatttgaa aaaccaggct ccgttaaagc agaagatatg accgaacttg
    16561 gtaaattcgt tcgcgagatc attgagaaaa atccagaaaa tttccgtata tttggaccag
    16621 atgaaacaaa atcaaaccga ttgaatcaag ttttcaaaac gacaaatcgc caatggatgg
    16681 aaaaaatcga accagaaaat gatgaatggc tttcgccatc tggtcgcgtc atcgacagtc
    16741 aattatcgga acaccaagat gaagggttct tagaagggta cgttctgact ggacggcatg
    16801 gtttttttgc aagttacgaa tcattcttgc gagtggttga ctctatgttg actcagcact
    16861 tcaaatggat acggaaatca catgaccttt cttggcgaaa agattatcct tcgctgaatc
    16921 tcattgcaag ctcaacagtc ttccagcaag atcacaatgg atatagccac caagatccag
    16981 gaatattgac acatttggca gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg
    17041 atgcaaatac attgctcgca gtcatggata aagcattccg ctcgtcagaa aaaatcaatt
    17101 tgatcatctc ttccaaacat ccgcgtgctc aattttactc agcagaagaa gcagccgtct
    17161 tagtgaatga aggcttgaaa atcattgatt gggcttcgac agcaaaagag gaggaacctg
    17221 aattggtcat tgctgctgca ggaacggaat ctaacttaga agcattagca gctgtaactt
    17281 tgctgctgga agaattccca aaactaaaaa ttcggttcat caatgtcgta gatttattga
    17341 aattacgtca tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt gatcaatatt
    17401 ttacaaaaga caaaccgatc ttatttgctt tccacggtta tgaaacattg gtccgcacga
    17461 ttttctttga tcgtcataac catcatttaa tgattcatgg ctacaaagaa aatggagata
    17521 tcacgacacc atttgacatg cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag
    17581 acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt tgcggaaaaa atggatcaga
    17641 aattacaaga acatcaaaac tatattcgtg aaaatggtat tgatctgcca gaagtattgg
    17701 attggaaatg gaaaaatctt gaccaataaa atgataaaat cgatcccgca aaacagaatt
    17761 ttttacattg tattatttgt caaattaag
//
LOCUS       DANJMA010000051        16981 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000051 DANJMA010000000
VERSION     DANJMA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..1238
                     /gene="scm"
                     /locus_tag="PWU31_002091"
     CDS             <1..1238
                     /gene="scm"
                     /locus_tag="PWU31_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL1084589.1"
                     /translation="DMNNRTIDLTDLSDTGFLLVNIDAEVLTMNTPLQIINPNDQVVV
                     FNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGNEMTDLTISAPFQGTILAP
                     NATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGTDTTDTSDSTDTSTTSDSS
                     DSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTD
                     TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDT
                     SASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTS
                     STTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGV
                     ILLVIGLRITFMSYKKSKR"
     gene            complement(1393..2379)
                     /locus_tag="PWU31_002092"
     CDS             complement(1393..2379)
                     /locus_tag="PWU31_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL1084590.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2402..2968)
                     /locus_tag="PWU31_002093"
     CDS             complement(2402..2968)
                     /locus_tag="PWU31_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL1084591.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2982..3398)
                     /locus_tag="PWU31_002094"
     CDS             complement(2982..3398)
                     /locus_tag="PWU31_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL1084592.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3855..5789)
                     /locus_tag="PWU31_002095"
     CDS             complement(3855..5789)
                     /locus_tag="PWU31_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1084593.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6070..7026
                     /locus_tag="PWU31_002096"
     CDS             6070..7026
                     /locus_tag="PWU31_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL1084594.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            7116..8219
                     /locus_tag="PWU31_002097"
     CDS             7116..8219
                     /locus_tag="PWU31_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL1084595.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            8232..9185
                     /gene="menA"
                     /locus_tag="PWU31_002098"
     CDS             8232..9185
                     /gene="menA"
                     /locus_tag="PWU31_002098"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL1084596.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9999..11345)
                     /gene="gor"
                     /locus_tag="PWU31_002099"
     CDS             complement(9999..11345)
                     /gene="gor"
                     /locus_tag="PWU31_002099"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL1084597.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(11479..12732)
                     /locus_tag="PWU31_002100"
     CDS             complement(11479..12732)
                     /locus_tag="PWU31_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL1084598.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12738..13661)
                     /locus_tag="PWU31_002101"
     CDS             complement(12738..13661)
                     /locus_tag="PWU31_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL1084599.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13882..16374)
                     /locus_tag="PWU31_002102"
     CDS             complement(13882..16374)
                     /locus_tag="PWU31_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL1084600.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(16381..16848)
                     /locus_tag="PWU31_002103"
     CDS             complement(16381..16848)
                     /locus_tag="PWU31_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL1084601.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         5033 a         3567 c         3019 g         5362 t
ORIGIN      
        1 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
       61 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
      121 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
      181 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
      241 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
      301 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
      361 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
      421 atggtacaga tactacagac acaagcgaca gtacagatac atctaccaca agtgatagct
      481 cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
      541 atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtgat agcacggaca
      601 catcagcttc aagtgacagt acagatacta cctctaccac aagtgacagc acggacacgt
      661 cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg gacacatcag
      721 cttcaagtga cagtacagat actacctcta ccacaagtga tagcacggac acgtcagctt
      781 caagcgacag tacggataca tctaccacaa caagtgatag cacggacacg tcagcttcaa
      841 gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct accactacaa
      901 gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct accacaagtg
      961 atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc acaagcgata
     1021 cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg aactttaact
     1081 taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt cctaaaactg
     1141 gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta atcggtttaa
     1201 gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa gataaaaaag
     1261 agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa tacaagaact
     1321 acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt tacgtatcga
     1381 acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa agagattctc
     1441 ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct tttctgtgta
     1501 agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga ttttgcgtac
     1561 ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta gtagctttgg
     1621 tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac gcatgtcttc
     1681 tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat catcgataat
     1741 ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga agcgctcgct
     1801 tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag ctgtcttacc
     1861 tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat catccatctg
     1921 acctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga gacttttcaa
     1981 ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt ctcctgcata
     2041 gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc gcagatccgc
     2101 ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga tagaagctgc
     2161 caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca atagctgata
     2221 ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgattgctt gttcgacagg
     2281 tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca attctttact
     2341 taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc ttgtgtgacg
     2401 attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca taatcttttt
     2461 ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc ccgattgcaa
     2521 tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact gtccatgatt
     2581 gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg ccggccgcac
     2641 taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac attccaaaat
     2701 aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg cctagcaaag
     2761 tagtcaggaa tagtcgtagc caaagtagta agaagctgtc tcgttttttc atggtaaaaa
     2821 tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca aaagaaaaag
     2881 gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca agtagcgaaa
     2941 tataaatcat tttttgtaat ttactcatgg tgtgtgaagg tctaataaat cagactgtct
     3001 tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa acagacgatg
     3061 gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc gcaatctgct
     3121 tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc gtgttcatct
     3181 ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt gatttctgtg
     3241 ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg aagaccaaaa
     3301 atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac atcccaaggt
     3361 ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct ttttccagat
     3421 taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct tgctttgagt
     3481 aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg gcttattacc
     3541 gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt gggcaacttc
     3601 aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat ttcggcttat
     3661 ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac agaggttgtg
     3721 acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta attttacata
     3781 aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc attcaaataa
     3841 cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta ccaccaatta
     3901 cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc acgtccggaa
     3961 ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta aaacatgcct
     4021 gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca tgtgaataat
     4081 ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt ttggaagaac
     4141 attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca gtctggtgac
     4201 ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa atgtgcagct
     4261 tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt tgcatcacca
     4321 gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca agggaacaca
     4381 ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa cacttcacca
     4441 ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa aatacgaagt
     4501 ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac gttgcgatca
     4561 tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc agaacgaata
     4621 tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa tccgcttaaa
     4681 tggatcttat caaataggtt agctaccccc catttggcac caatagaaac cataaatcct
     4741 tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac gatattcgct
     4801 gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg tgttcctggg
     4861 aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc gtagcctttt
     4921 gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa tccaaagtca
     4981 gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt cggaatgttg
     5041 cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc attcaataat
     5101 aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt tcgatccaac
     5161 atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt tggatctaat
     5221 ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat ttcaataccg
     5281 gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc atcatctggt
     5341 tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac agaatcttca
     5401 aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc attcggctct
     5461 ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt cttgacgatt
     5521 tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt cttttgacga
     5581 gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc gccagctact
     5641 tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt aattgctaca
     5701 tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc atatcctgct
     5761 cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga ctatctatat
     5821 aatgaatata tgataattct tttagtttga tacttattac acaatctcca tatattatat
     5881 agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta aaataaagcg
     5941 ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt atttatcaaa
     6001 aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa gaaaaattgt
     6061 ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt aactggattt
     6121 gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga caacggaagc
     6181 tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag cacaacagaa
     6241 tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa attagaagaa
     6301 aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga cggcaaaatc
     6361 actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa agatgcagaa
     6421 tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat tcctgagttg
     6481 aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac tggagctact
     6541 cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc tcaagctggt
     6601 aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac ttacaagctt
     6661 gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt tgctggcggc
     6721 aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa acaagatgat
     6781 acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac ttacatccct
     6841 actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc tgctaatgta
     6901 gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc acaacaatta
     6961 gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat cgtaacagaa
     7021 aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc agattaggca
     7081 gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta ctattttcct
     7141 taatcacact gggcatctct tttatggtgc taactggctg cagcaactca gcaaataaaa
     7201 caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct gatgaacagt
     7261 tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa tcagcattga
     7321 aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat caaaaaggtt
     7381 ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt tctgatgacg
     7441 tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc ggattcgata
     7501 tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc agaaaacctt
     7561 cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc gaactaaatg
     7621 acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta ggtgcaatcg
     7681 ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga gtgaccactg
     7741 ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga ggcgaaaacc
     7801 aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt ttaggttcta
     7861 ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat ttagaagtag
     7921 atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc gacaatgata
     7981 tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg tctacagcag
     8041 tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg aataacggga
     8101 cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca atcaaagaca
     8161 ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa ctaaaataag
     8221 gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga acaaaggtgg
     8281 ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt catgagatac
     8341 attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta gcgactaccg
     8401 ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag tatgaagaaa
     8461 atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt ttagctatga
     8521 ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg cttttacttt
     8581 tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg attcctttat
     8641 ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga atttttgcca
     8701 tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc aacttttcat
     8761 ctggacaatt tgctttggta ggaaatcttt gggctatagc agcaatcgta ttagcttcct
     8821 tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg gatcttgacc
     8881 gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct gcgggaacag
     8941 tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg ttgttgagcg
     9001 gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg aagaagcatt
     9061 tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc atcaaaaata
     9121 tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt atgcaagcca
     9181 tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa gaacagccaa
     9241 ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
     9301 tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat taagaaaaat
     9361 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga gaaactggct
     9421 tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta ttagagaaac
     9481 aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga aggacagctg
     9541 attgaacact atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc
     9601 ttttttcact tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa
     9661 ctatatggta ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac
     9721 acttttttaa acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata
     9781 ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata
     9841 atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat
     9901 aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt
     9961 ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct
    10021 tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc
    10081 ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag
    10141 ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa
    10201 tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg
    10261 ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta
    10321 aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa
    10381 cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc
    10441 acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata
    10501 aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt
    10561 acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc
    10621 accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt
    10681 attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta
    10741 agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccaca aaatacacct
    10801 gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt
    10861 aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt cattttactt
    10921 gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg
    10981 atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct
    11041 aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga
    11101 ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca
    11161 attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg
    11221 acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct
    11281 gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt
    11341 tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt
    11401 ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg
    11461 gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg
    11521 tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc
    11581 ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc
    11641 gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc
    11701 atcataagat aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa
    11761 taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc
    11821 tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat
    11881 ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt
    11941 cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc
    12001 gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt
    12061 taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga
    12121 ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg
    12181 accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt
    12241 tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa
    12301 cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa
    12361 atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac
    12421 aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc
    12481 atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc
    12541 aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc
    12601 atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt
    12661 ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat
    12721 ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct
    12781 aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct
    12841 tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt
    12901 acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga
    12961 taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca
    13021 tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga
    13081 cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt
    13141 ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg
    13201 agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca
    13261 actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca
    13321 tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta
    13381 tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca
    13441 tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac
    13501 ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc
    13561 aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct
    13621 gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac
    13681 ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt
    13741 tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa
    13801 aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat
    13861 tcagctacac aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac
    13921 attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc
    13981 tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat
    14041 tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg
    14101 tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat
    14161 ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat
    14221 tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat
    14281 ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat
    14341 ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct
    14401 acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc
    14461 tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt
    14521 ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg
    14581 gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg catcttcact
    14641 tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc
    14701 aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg
    14761 gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag
    14821 tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc
    14881 agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg
    14941 ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg
    15001 tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg
    15061 tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc
    15121 caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg
    15181 agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc
    15241 cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc
    15301 aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc
    15361 gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc
    15421 taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat
    15481 taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat
    15541 gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc
    15601 catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat
    15661 tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac
    15721 agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg
    15781 accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga
    15841 atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg
    15901 acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg
    15961 acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc
    16021 atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt
    16081 cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac
    16141 tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat
    16201 ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac
    16261 cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc
    16321 ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc
    16381 tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt
    16441 atgatcttca tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt
    16501 tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt
    16561 tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc
    16621 tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag
    16681 ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca
    16741 gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat
    16801 tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc
    16861 ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct
    16921 ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat
    16981 c
//
LOCUS       DANJMA010000052        16740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000052 DANJMA010000000
VERSION     DANJMA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..421)
                     /locus_tag="PWU31_002104"
     CDS             complement(<1..421)
                     /locus_tag="PWU31_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDL1084602.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSP"
     gene            714..2150
                     /locus_tag="PWU31_002105"
     CDS             714..2150
                     /locus_tag="PWU31_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1084603.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2320..3516
                     /locus_tag="PWU31_002106"
     CDS             2320..3516
                     /locus_tag="PWU31_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1084604.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(3578..3763)
                     /locus_tag="PWU31_002107"
     CDS             complement(3578..3763)
                     /locus_tag="PWU31_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL1084605.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            3994..7308
                     /gene="dnaE"
                     /locus_tag="PWU31_002108"
     CDS             3994..7308
                     /gene="dnaE"
                     /locus_tag="PWU31_002108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL1084606.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7476..8438
                     /gene="pfkA"
                     /locus_tag="PWU31_002109"
     CDS             7476..8438
                     /gene="pfkA"
                     /locus_tag="PWU31_002109"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL1084607.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8512..10296
                     /gene="pyk"
                     /locus_tag="PWU31_002110"
     CDS             8512..10296
                     /gene="pyk"
                     /locus_tag="PWU31_002110"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL1084608.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10331..11233)
                     /locus_tag="PWU31_002111"
     CDS             complement(10331..11233)
                     /locus_tag="PWU31_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1084609.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11419..11988
                     /locus_tag="PWU31_002112"
     CDS             11419..11988
                     /locus_tag="PWU31_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL1084610.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12046..12225
                     /gene="rpmF"
                     /locus_tag="PWU31_002113"
     CDS             12046..12225
                     /gene="rpmF"
                     /locus_tag="PWU31_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1084611.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12395..13816
                     /gene="gndA"
                     /locus_tag="PWU31_002114"
     CDS             12395..13816
                     /gene="gndA"
                     /locus_tag="PWU31_002114"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL1084612.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14009..14695
                     /locus_tag="PWU31_002115"
     CDS             14009..14695
                     /locus_tag="PWU31_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084613.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            14692..16197
                     /locus_tag="PWU31_002116"
     CDS             14692..16197
                     /locus_tag="PWU31_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1084614.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5436 a         2883 c         3517 g         4904 t
ORIGIN      
        1 taggagaaag caaattcttc tctcctggac gagaagaagc aaaaattttt tcatcaaaga
       61 aatcaaaact gtcaataaaa aaagtatcac gattttcagc agcgataaaa tctaaattta
      121 gctgcttttt ttgcgataag atttcaaaag cgatttcacg attgatcaac gtttctcttt
      181 caccatccca gtgctgatta gggatgtgta ctaatgcgcc gttaaaatta accatcggtg
      241 ttgttaattc taattgttgg taaaactgat ggctcattcg gaatgggcgt cctgtagcaa
      301 tactgacatg atgaccggca ttgattgctt tttttagagt ctcttttgtt ttagatgtaa
      361 tcatcgaatc ttgattgagg gtagttccgt ccagatcgat ggcaattaat tttttattca
      421 ataagggtcc ttccttcctc aactagcatt tgttttagtc tagcatattt taggtaatta
      481 acaagagcaa tcagctattc ctaacaaaaa ctacagttat ggctatataa atagctaaac
      541 ataagctaaa cacgaacgtt aagtattatt ttcgcttttt ttgttaaaaa aacgttgaaa
      601 agcgaatgaa acgtctatat aatttgaaat tgttcgatgg atttgcccta ttttacttat
      661 tttttattat tatagttttc catcagaaaa aaattcaggg agtggaactt tttatggaaa
      721 agtttttcgg tttaaagaaa aacaatacaa atgtatcaac tgaaataatg gcgggagtaa
      781 cgacattctt tgccatgagt tatatcttat tcgtaaatcc gacgattttg tcagcatcag
      841 gtatgccctt ccaagcagta tttctagcga caatcattgc atcgattatt ggtacgctgg
      901 taatgggatt gttcgcaaat gtaccatatg ctcaggcacc tggtatggga ttgaacgcat
      961 ttttcacttt tacggtcgtt tttgggcttg gttattcttg gcaacaagcc ttggcaatgg
     1021 tgtttatttg cggattaatc aatattttta ttactgtcac aaatattcga aaaatgatta
     1081 ttcgggcgat tcccgaaagt cttcaacatg cgattggtgg cgggatcggt attttcgttg
     1141 cgtatgtcgg aatcaaaaat gcaggattct tatctttttc agcagaccag tcagctattt
     1201 cgagctctgt cgttgaaggc ggaaaagcga caaatgttac gataaatggt ggaatcgtcc
     1261 ctgcgttagc caactttgac aacgctccta tcttattggc tgtgattggt ttggtactga
     1321 cagccatttt agttgtaaaa aatgtccgtg gggctattct tatcggtatc gtagcgacca
     1381 cagtccttgg tatttttatg ggtgttgttg atttaagttc cattgattgg caaacgaatt
     1441 cgttaggtaa ttcatttaaa gaattaggaa caactttcgg tgcagcattt ggttctgaag
     1501 gcatgcaatc tttattcagt gattcttcca agattcctca agtattgatg acgatcatcg
     1561 cattcagttt atctgatact ttcgatacga tcggaacatt tatcggtaca ggacgccgca
     1621 caggtatttt ctcaaaagaa gacgagatcg cgttggaaga tggtcgtggg ttcaaaacaa
     1681 aaatggataa agcactattt gccgatgcga tcgctacttc tgtgggggct atctttggta
     1741 catcgaatac aacaacttat gtcgaatctg cagctgggat cggggccggt ggacgtactg
     1801 gtttgacttc agtagtagtg gcagtgctat tcgcattaag tagtttattc tctccactga
     1861 tcgccattgt accaaatcaa gcaactgcgc cagcattgat tttagttgga gtaatgatga
     1921 tggcttcttt tgcagacatc aaatggctag atatggaaga agctttaccg gccttctttg
     1981 catcgatctt tatgggattg tgctatagca tttcctatgg gattgcagca ggatttattt
     2041 tctatacaat tgtgaaagta atcaaaggaa aatacaatca agtttctgtt gctttgtgga
     2101 tcgttgatat tttattcatt atcaacttca tcattctagc aacaatttaa ctaaagagaa
     2161 gggattggaa cagtatttgt tctgatctct ttttgtttta cataaaaaga acgttacata
     2221 taaaatgacc tttgcgggat ttttctcttt ttactggcga gatcgttgct tgtttattcg
     2281 aaaataggta tgataaaaga gacgaaagag ggatgataga tggagattaa ttggagaaaa
     2341 aatttagtga ttgcgtggat tggctgtttt ttcactggtg caagtattag tttagttatg
     2401 ccattcatac cagtttatgt tgagcaacta ggaacaccaa aagatcaaat cgaattgttt
     2461 tcgggactag caatctctgt gactgctttt gcatctgccg tggttgcacc aatctgggga
     2521 aatctagctg atcgaaaagg ccgaaaactc atgatgatcc gtgcagcagc agggatgaca
     2581 ttaacaatgg gatctttggc ttttgtacca aacgcgtatt ggcttttgat catgcggttt
     2641 tggaatggga ttttgtctgg atatattcca aatgccacag cgatgattgc ttcacaggca
     2701 cctagagaaa agagcggatg ggcgttagga acattgtcta ccggagcgat tgctggaaat
     2761 ctgatcggac cttcaatggg cggtgcactg gctcagtggt ttggtatgga aaatgtattc
     2821 ttgatcacag gggctttgtt gatgatcaca acggtattga ccattttctt agtcaaagaa
     2881 gatttccatc caatcgagaa aaaagatctg ataagcacca aagaaatttt ttcaaaaatg
     2941 gatcatctct ctattttgat cggtctcttc attacgacct tgattttaca gataggaatc
     3001 actacgatca gccctatttt gaccttatat attcgtgaat tgagcggaag tacagaaaat
     3061 atcctttttg tcagcggctt gattgtttcg atagctggtg tttccgctgt cttttcttca
     3121 ccgaaactag gaaaactagg ggataagatt gggaatcaaa aagttctaat tggaggactc
     3181 attttatcat tttgctgtta tttacctatg gcattcgtca ctacaccgct ccaattaggt
     3241 atacttcgct ttattttagg cttttcaact ggcgctttga tgccttctgt caatacactg
     3301 atcagtaaga ttacgcctca agaaggagtc agccgaatct atagctacaa tcagatgttt
     3361 agcaactttg gacaagtttt aggtcccatg ctaggatcga cagtcgcgca tgcttataat
     3421 tacagtgctg tttttattgt gacaagtttg tttgttctgt caaatattct tctttcttta
     3481 ttcaactttc gtaaagttct tcgcagcaga ttataatggt ggtgaggagt agaaaataaa
     3541 aaagagcttg agaaatctca agctcttggc tgttttttta cgaatgggca gaggtttctt
     3601 ttcttggcgc aactgcacca atcaaaagga taatgacacc cgcaatgaat gaaatgattg
     3661 ttgtcaaagt aaaatcatat gttccgcttg ttaatgcagc accaatataa ccgactactt
     3721 gacctagaac gacagcccag aataatgtaa cgatatatcg cataaagaac acctcttttc
     3781 tatcttaatt ttaccagatt ttctttgaat gtaaagaaac gagaccaaat ctcaataaaa
     3841 tttgagttct tgcgaaagaa ctcattccta cagattacaa gcaagcaaat gaagtggaat
     3901 aatcaacttc agcgcctgct atcttaatga aagtaaaagg atcaaacctt ttaacgctag
     3961 tgaattttaa cgaaaaaaaa ggcggaatgc aagatgtttt tcccacaact ttatacaatt
     4021 acttcttatt ctcttttaca aagtacgata cgtattcgag aatatgtaca agaagcaaaa
     4081 aaacaaggct acacttcatt agccataacg gataaagatg tcttatacgg tgcacctgaa
     4141 ttctatcgta tttgtgtaga agaaggagta cagccaatca ttggattaca tcttagttat
     4201 acttatgaag aaaaaaatta tggcttgctt gcgtacgcaa aagatgaaat aggttatcag
     4261 cagctgatga aactttccag ccgaaaaatg atccaagaaa aattggaact tgcagaaatg
     4321 acagaaaccg accatctagc gtttgttgtt ccggcaaatc attctgtttc tgaggctttc
     4381 aataaagagg ataacttttt cggtcattgg cagtttttac ttgaaaatgt ttttactaga
     4441 aatttttata tcggcttgac agaaaacacg cctgatcaat tgcctgaatg gtatcagtat
     4501 gtgataaaag aaaattattc gctttgtgca ttacatccca tcgctagttt acaacaagaa
     4561 gaactttttg cacttgaagt gatgaaacat atcaaagagg gaagccaaat acctttagaa
     4621 aatttatctg catctttacc actaacagca aatgtctatc ttctttcaga acaagaaatg
     4681 atcaaacgat tcgaaaaagc gggtctttcc caagctgtaa taggcgccca gcgtttagct
     4741 gaatcgattt catttgaatt ccagttccat cagaaattac ttccgcatta tccagttcct
     4801 aatggaaaac aagctggaga atatttaaga gagttatgct attcgcaagt ggaacgccga
     4861 atcactgact atacaaagga ataccaagaa cgtttggact acgagttatc tgtcatccat
     4921 caaatgggat tcgatgatta tttcttgatc gtatgggatg taatggcttt tgctcataga
     4981 aacaaaatcg ttaccggtgc aggtcgtggt tctgctgcgg gttcattagt agcctatgtg
     5041 ttagaaatca cagatgtgga tccattagaa tatgatttgc tgttcgaacg ctttttaaac
     5101 cccgaacgct ttacgatgcc agatatcgat ttggatattc ctgacaatcg acgtgaagaa
     5161 gtattgaatt atgttcgaga aaagtatgga caatatcata tggcacaaat cgcaacattt
     5221 ggcacaatgg cagcaaaaat ggttcttaga gatgcagcta gagtgtttgg tttatcgcaa
     5281 agtgaagcaa accgctggtc aaaagcaata cccaatcaat tgaaaatcac attgacccaa
     5341 gcttataaaa cttcgcatca attagtagaa ttagttcagg caagcgaaaa aaaccaactt
     5401 ttattcaaaa ctgcagtgat tttagaagga ttgccaagac atgtttcgac tcacgcagca
     5461 ggtgtagtta tcagcgataa gaatctactt gaactagttc cgctccaacg cggttctgat
     5521 gaagcttttc tgacccagta tacgatgaat gaggtagaag caatcggtct tttgaaaatg
     5581 gactttttag gactacgcaa cttatcgatt attgatttta ctttgaaagc tgttaagcga
     5641 gtagaagggt atgagatccg attaaaagag atcccgctaa atgatgcaaa aacgctgctc
     5701 ttgtttcaac gaggcgaaac ctcaggagta tttcaatttg aatctgctgg tatccggaac
     5761 gttttacgaa gattagatcc agaaaacatc gaagatattg ccgcagtcaa tgcattgtat
     5821 cgaccaggcc ctatgcaaaa tatagatacc ttcattgcca ggaagaaagg aaaagaagcc
     5881 attcgctacc cagatgattc actgatcccg attttgaaaa acacatatgg agtgatcgtc
     5941 tatcaggaac aaatcatgca gatcgcctcg actatggctg gatttacatt agggcagtca
     6001 gatattctgc gacgggcagt gagtaaaaag aaaaaagacg tattagatga agaaaagtcg
     6061 cattttgtat caggagcatt acaacaggga tattcggaaa aaacagcagc gaccgtatat
     6121 gaatatatcg aaaaatttgc aaactatgga ttcaatcggt cacatgcatt tgcttattca
     6181 ttcgtgggat tccaaatggc gtatctaaaa gcccatcatc caggtgcttt ctttaccagc
     6241 ctgatgaatt ctgttcggca taacacatca aaattgaaag aatatattgc agaagcacgt
     6301 aaaaataaac tcaaactttc ctcgccgtcc attaatcaaa gtaattccag ttttgagttg
     6361 gtcaataagg aagaaatcag attcggactg acagcgataa aaggtattcg gcgtgatttt
     6421 gtagaagata tattaaacga aagaaaacaa aatggcccgt tccagtcagt ggatcaattc
     6481 ctcatacgga tcgataagcg ttggctgaaa ttagagttac tgcaatcact gacagcagta
     6541 ggagtttttg atgaattatc tcctaacagg aaacaattga tgcttgatct cgaaggaaaa
     6601 atccaaaata tcgtttacag tggtggaagt cttgacttgc tgggaatcat ggcattaaaa
     6661 gaagaagaag tcgctgacta tacactagaa gaacgcttga agctggaaga ggagtactta
     6721 ggtatttatt tgtcaggtca tcctgcagaa gggtttgatc gtcttcgttt agctaaacaa
     6781 gttcagctga ttacagatat cgtccctaat caaccagtca atttattagt gatgatcaag
     6841 gaagttagag aaattcggac aaaaaaagga gaactgatgg cctttgttga aggaaccgac
     6901 agtagtggag aaatttctgt cacagttttt ccaactcttt atcgtaaata tcgtaatttg
     6961 tttgccgttg atcgagtgat ctttgttcaa gggcggagcg aaatcagcaa atataatcag
     7021 gaactgcaac taattggaga aagctttgct gatcctaacc agttagaagc ccagtatcct
     7081 tcacaaactt gctatttgcg tattacagaa gaacgggaag atcccgagat aatgaaacaa
     7141 ttacaagtga tttttcagaa acacccagga tatataccag tagttatgta tcacgaaaaa
     7201 acgcaacgaa aagtagttct gtcagatgct tattgggtgg atgacagcaa gagtataaag
     7261 cggcaactta tttatctgct tggcgaagat aatgtggttt tcaaataact tacggtaaaa
     7321 aatactactt gctttcattt tttttcattt ttttatccgt aaatcaagac aagtgagaaa
     7381 aataatgtta gaatgttagt cgtggttaac gcaaaaatgg ttgtaaacct aagtcatact
     7441 taggataaag tattttaatg aggtgaatgt ttattatgaa acgcatcgga attttgacca
     7501 gtggcggaga cgcacctgga atgaatgcag ctgttcgtgc cgttgttcgt aaaggtattt
     7561 acgaaggact ggaagtatac gggatcaatt atggttttgc cggtttagtt gctggagata
     7621 ttcgtcgtct tgatgtggca gatgtaggtg acaagatcca acgtggtggt acattcttgt
     7681 attctgcacg ttatccagaa tttgcaactg aagaaggtca attaaaagga atcgaacaat
     7741 tgaaaaaatt cggaatcgaa ggtctagtgg ttatcggagg agacggctct tatcatggcg
     7801 caatggcttt gacaaaacgc ggtttcccag cagtaggtat tcctggaaca atcgataatg
     7861 atattcctgg gactgatttc acaatcggtt ttgatacagc aatcaataca gtactagaat
     7921 caattgaccg tattcgtgac acagcgactt ctcatgttcg tacattcgtt atcgaagtaa
     7981 tgggacgcaa tgcaggagac atcgctttat ggtcaggtgt ggctggcgga gcggatgaaa
     8041 tcatcatccc agaacatgat tttgatatgg cttcagttgc taaaaaaatc caagaaggcc
     8101 gcgatcgcgg gaagaaacat tgcttgatca tcttggctga aggtgtaatg ggcggaaatg
     8161 aattcgctga gaaactttca gaatttggtg actatcatac acgtgtatct atcttgggac
     8221 acgttgtccg tggtggagca ccaagcgcac gcgaccgcgt gttggcaagt aaattcggtg
     8281 cttacgctgt cgaattgctt caagaaggaa aaggtggatt atgtgtcggt atccatgaca
     8341 acgaaatcgt tgcgtctgac atcattgata cacttgaaaa caacaaacat aaaccagact
     8401 tatcactata tgatctaaat aattcacttt cattttaaga gtgaaagaaa ccaagctatt
     8461 taagctgtgt acagctttaa ataaacaaaa ttgaatggga gagtttttta aatgaaaaaa
     8521 accaaaatcg ttagtacatt aggaccagca agtaactcag tagaaattat ttctcaattg
     8581 attgaatctg gggcaaatgt cttccgcttt aatttttctc atggtgacca tgaagaacaa
     8641 ttatcacgta tgactatggt ccgtgaagca gttaaaaaga caggaaaaga tgtcggtatt
     8701 cttttagata caaaaggagc tgaaatccgt acaactgttc aaggcacaac agaagctgat
     8761 tttggtcgtg caggatacat caaatttgaa gtaggagatc aaacacgcat ctctatggat
     8821 cctgaacatg taggttcaaa agaaaaaatc gcggttactt acccaggatt atttgacgat
     8881 gttcacgttg gcggacacat tttatttgat gatggtttga tcgatatgca aatcattgaa
     8941 aaagacgaag caaaccgtga attagtcgta gaagttaaaa atgcaggtat gctaggatca
     9001 cgtaaagggg taaatgcacc aggcgtttct attagtttgc caggtattac tccaaaagat
     9061 gctgacgata ttcgttttgg tttagacaac gatatcgact ttatcgctgc aagttttgta
     9121 cgtaaagcac aagacgttct agatatccgc gaaatcttag aagaaaaaga catgactcat
     9181 gttcaaatct tccctaaaat cgaatctcaa gaaggtatcg acaacattga cgaaatcatc
     9241 aaagtttctg acggtgttat gattgcccgc ggagatatgg gtgttgaaat cccagctgaa
     9301 ctagtaccaa tggttcaaaa acgcattatc aaaaaatgta atgctgctgg tatcccagtt
     9361 atcactgcta cacaaatgtt agaatcaatg caagaaaacc cacgtccaac acgtgcggaa
     9421 gcttctgacg ttgctaacgc tgtatttgat ggcacagatg caacaatgct atctggtgaa
     9481 tctgcaaacg gggactaccc agttgaagct gtttcaacaa tggcacgtat cgaccaagaa
     9541 gctgaaaatg cattatcaga attaggtaca ttccaattga acgaattcga taaaacagat
     9601 gtaactgaaa caatcggttt atctgtagct cgtgctgcaa aaaatctagg cgtgaaaaca
     9661 atcgttgctg caactgaatc aggatacact gcaaaaatga tttcaaaata tcgtccagat
     9721 gcagatatct tggctgttac ttttgacgaa cgcacaaaac gtggtttgat gttaaactgg
     9781 ggcgtttacc caacagttgc tgaaaaacca tcaacaactg atgaaatgtt tgaactagca
     9841 gctaaaaaag ctgttgaatt aggtttcgca tcagaaggcg atttaatctt gatcactgct
     9901 ggtgtaccag ttggtgaacg cggaacaact aacgtgatga aaattcaaat gattggttct
     9961 aaattattag aagctcaagg cgttggacaa aaatctgtcg ttgcaaatgc agttattgct
    10021 tcatctgctg aagaagctgt aaaaaaagca aaagacggaa tggttctagt tgtaccatca
    10081 actgacaaag aatatatgcc tgcaatcgaa aaagcagctg ctgttatcgt tgaagaaggc
    10141 ggattgactt ctcacgctgc agttgtagga attgcacaag atattcctgt aatcgttggt
    10201 gctaaaggcg caacaacaac aattacagaa ggtgaattga ttaccgttga ttcacgtcgc
    10261 ggcatcgttt accgtggcga aacaacagct atctaattta gcttaataaa aaataaaaac
    10321 agctcttctt ttacgaagag ctgttttttt gtgctgtaac gaattcatgg ataacttgga
    10381 ctacttgttc ttgaagaggc gtgacgatct gctgtgttcg ttttgccaat gaaatcaaga
    10441 agcgaggctg aacttcatct gaaagaggga ggcaaactaa atgatcttca ggatggacag
    10501 caatctctgc caaaaaaccg atacccacat tttctcggac catactcttt aaaatcgtca
    10561 agtcattgct ttgataaaca acttttggat gagtatgtgt ttgtttagaa agcttagtga
    10621 atgctgcggg gtggacataa tgttcgttca atagtacaaa ctgttcatct gctatatctt
    10681 tgaaagagac agtatcctgc ttggaaagag gatgtgtagg tggcacgacg atcatgaaac
    10741 gtttgtccaa aagtaactct gtttctaaat attgatccac gattggctgt gttgagccta
    10801 atacagctaa atcgatcctc ccttgtcgga tcatcccgta taagtcccga gagcctccat
    10861 cggtaatctc aatttgattc atcaattcat gcttgataaa gtaacttgac agtttaggaa
    10921 agtattcatt gccgataata ggaggcatgc caaaacgaag ctttttcttt tgcaactgag
    10981 tgatttcagc tcttgcagta gaaatctctt ggagaatggt ttgtgcatgg agaaacagct
    11041 gctttccaga aggcgtgacg attaattcgt gatgcgatcg atctcttgta ataagtacgg
    11101 tgtcaaactc tttttcaaga cgttgtaatg catatgtaat agtaggttgg ctcacacgga
    11161 aataattagc cactttcgta aaattttttt gtttgacgat ttggacatag tattcgatat
    11221 ctttcgtgtt catgaagatc atccgctcct ttaatggaat aatgattatt gtaaatgatt
    11281 ctgaggatga tagcaaacag aggctttcta ataaagattt ttctgttttt ttatgaactg
    11341 caaggtgtca agaaaatttc attgacaaca tgctttttgc cctgtataat tgcttttgtt
    11401 gcttaggagt gatgaaaaat gaaatggtct ttattggaat tgagaaaata ccaggagaca
    11461 ccgttaactt ttcatgagac gctcgacttg aaagccgctc tcatgaagcg agacaattta
    11521 atacttgatg ttgcgccagt ggaagtcgaa ggtttggtct cagttgataa gaaaggctat
    11581 atcgttcatt acactgtgca gacgactttg acagttcctt caacacgttc actagaacct
    11641 gttgaactgc ccatggattt ttctgtagac gaagtattca tgacagaaga acaattccaa
    11701 agtagagatg aacaaatgtc tgcggatgag attttgctaa ttgaaggtcc attattgaat
    11761 ttagcagagt cgattgaaga caacatctta ttggcgattc caatgcgggt attgacaaaa
    11821 gaagaggaag cagcaacaga acttccaaaa ggcaatgatt gggaagttgt ttccgaagaa
    11881 gaataccagc gcagaaaaat ggaagaagcc gagaataaaa ttgatcctcg tcttgcaaaa
    11941 ctatctgaac tttttgattc taaatcaaaa gacgacgata acaagtaaga ttggaatata
    12001 ttgtgtctaa tttagataca agactttaca aggaggtgta gcaaaatggc agtaccagct
    12061 agaagaacat caaaagctaa aaaagcaaaa cgtcgtacac actacaaatt gacaatcaaa
    12121 gggttaaacg aatgtccaaa ctgtggtgaa atgaaaaaaa gccatcacgt atgtgcaaat
    12181 tgtggttact atgatggtaa ggacgtaatg tcaaaagaag cttaatacat tttaattaga
    12241 aaatgtaaaa cctcaaggct aaccatggag ccttgaggtt tatttttttg tagtcggtaa
    12301 ataaaaaata tgttgttctt tagtttgcaa tgtagccgtg ttaagctata atgtaagaga
    12361 ttagggagaa atcccgtatg taggaggagt tcatatgtca aaacaacaaa tcggcgttgt
    12421 cggtatggcc gttatgggta aaaatctagc cttgaacata gaaagccgcg gctattcagt
    12481 ggctttattc aatcgtacag gggctaaaac aaccgctgta gtagaagaac acccagataa
    12541 aaatttcaaa gcaacatata caatcgaaga atttgtagaa tctatcgaga aaccacgtcg
    12601 gatcttgcta atggtcaaag caggaccggc aacagatgca acgatccaag agttgcttcc
    12661 tcaccttgat aaaggcgata ttttgattga cggaggaaat acattcttta aagatacgat
    12721 gcgtcgtaat gaagagttag ccaactctgg catcaacttt atcggtacag gcgtttccgg
    12781 tggtgaagaa ggagctttaa aaggaccttc aatcatgcct ggtggacaaa aagaagccta
    12841 tgcgctagtt gcaccaatct tagagcaaat ttctgcaaaa gcagaagacg aagcaccatg
    12901 tgtcacatat attggcccaa atggagccgg acattatgtg aaaatggtac acaacggtat
    12961 tgagtatgga gatatgcaac tgattgctga atcttatgac ttaatgaaga atattttaaa
    13021 cctttcagta gaagaaatgg ctgatatctt taaagaatgg aatcaaggcg aattagacag
    13081 ctatttgata gaaatcactg ctgacattct gacacgtaaa gacgatgaag gaacaggtaa
    13141 accagtagta gatgtgatcc ttgatgcagc tgggaataaa ggaacaggta aatggacaag
    13201 ccaaagcgcg cttgacttag gtgtgccact tccattgatc actgaatctg tatttgcccg
    13261 ctatatttct gcttataaag aagaacgcgt acaagcaagc aagattttat ctcgtacgaa
    13321 cgattttgag ttcacgggag acaaaaaaga attggtcgaa aaaatccgag aagcgttgta
    13381 tttcagtaaa atcatgagtt atgcccaagg atttgctcaa ttacgtgtcg cttcaaaaga
    13441 atttgattgg gacttgccat ttggtgaaat cgcaaaaatc tggcgcgctg gatgtatcat
    13501 ccgagctcga ttcttgcaaa aaattacgga tgcctatgac aaaaatcctg agatcgaaaa
    13561 tttattgcta gatgattact tcgttgaaat cacgaaaaaa tatcagcaat cagtcagaga
    13621 cgtcgttgcc ttggctgtcc aagctggcgt acctgtaccg actttctcat cagccatcgc
    13681 ttacttcgat tcatacagag cagaacgtct gcctgcaaat ataatccaag cccaacgcga
    13741 ttactttggc gcacatacct atgaacgtgt cgacaaagaa ggaatcttcc attactcatg
    13801 gtaccatgaa gaataatagt ctttcggctg ttaggaaatg agaaaataaa aagaggacga
    13861 aagagctgaa gtcgattgtt atgagtagac ttcagctctt ttttaaaatt gctatccatg
    13921 ataaaatgac tatagatttt taagcatatt atcggtataa tgatagagtt tttaaaaagc
    13981 cagtagaata gaaaggataa aataataaat gagcaatatt ctaatcattg aagatgaaaa
    14041 gaacttggca cgatttgtcg agctagagtt gaagcatgaa gggtataacg cagaagtcca
    14101 ctacaacggc cgaactggtt tagatgcggc attaaataat gagtgggatg ccatattgtt
    14161 ggatctgatg ctcccagaat tgaacggttt agaagtctgc agacgtgtac gccaagtcaa
    14221 aaatacacca atcattatga tgactgcaag agattctgtg atcgatcgtg tatctggact
    14281 tgaccacgga gcagatgact acatcgtcaa accatttgct atcgaagaat tattagcacg
    14341 tcttcgtgca ttgttgcgtc gaatcgatat tgaaggcgat aaaaatgtgg ctaaacaaac
    14401 tactattact tatcgcgatt tgactatcga aaaagaaaac cgtgtcgtac gtcgcggaaa
    14461 tgaagtaatt gaactaacaa aaagagaata cgagttgctg cttactttaa tggaaaacgt
    14521 gaacgtggta ttagcacgtg acgttttact aaataaagtg tggggatacg aaacagaagt
    14581 tgaaacaaac gtagtggacg tatatattcg ttatcttcgc aacaaaattg acgttcccgg
    14641 ggaggaaagc tatatccaaa ctgtacgcgg aacaggttat gtgatgcgct cgtgataaag
    14701 ccgaaaaaat cggaaaaaag agaactgaaa gggccctcct tgacaatcaa gtgggccttt
    14761 gcgagttctt tcttcatatt cgttgtgttt acaattttcg ctgtcattac ctataaatca
    14821 tctatcaatc tgattgtggc aaaagaaaga aacaatgtag agcgtacgat ttcagaagta
    14881 gcttctcgtt tggctaactc ggatgaagaa ctgacattag taaatacgta tcgtaatctg
    14941 acgaaagcga caaatgacgg agatgaggtc gtagatcata cttcactggt cgaaggaaac
    15001 ttaatgaaga tcgattcctt tatagctgag ctagggcagc catcaatgga tctttatgtt
    15061 tatgatttgg acgagaactt gatttttaaa acccatgaaa acagtcgaaa cctgattcag
    15121 actacacgta aagttccaac gatcgttaca ctagaaggaa aaactggctt tttatctatc
    15181 cagcctatat attctaaagg tacaagagaa aagatcggct atgcgcaaag cttctatgaa
    15241 ttatcttctt tctatgatat tcgaaataag ctattattga ctcttattgt tttagaagtt
    15301 gtttctttga tcttaagtag tattttaggt ttctttttat cttcttattt cttaaaacca
    15361 ctcaaggtac tcagagatac aatggatact atacgtaaag atccgcaatc agatatccac
    15421 atgcctgaaa tcgacacaaa tgatgaattg gcagatttag ctgaaatatt caatgagatg
    15481 cttgaccgta tgcgtttgta tattgaacaa caagagcaat tcgtggaaga cgtatctcat
    15541 gagttaagga cacctgttgc aattattgaa gggcatctta gtctgctaaa tcgttggggg
    15601 aaagatgatc ctgagatatt agaagaatca ttaagcgcca gcatgcaaga aattagccgt
    15661 atgaaaagct tagttcaaga gatgctagac ttatcaagag cagaacaagt agatgttcat
    15721 tatgcgaatg aaacaactaa aggaaaagaa gtcacctatc aagtttataa taatttcaag
    15781 atattatatc cagactatgt gatcacttta gatgatgatt tgacaaaaga agtgaccctg
    15841 caaatttatc gtaatcactt tgaacaatta atcattatca tattggataa cgcagtaaag
    15901 tattcaacta cacgaaaaga agtccatatc tctatttctt ccactctgaa tgaatttgag
    15961 atcgcgattc aggactttgg cgaaggaata cccaaagaag accttaataa gatcttcaat
    16021 cgtttttatc gagtggataa agcaagagct agaacgaagg gtggaaatgg tctggggttg
    16081 tctatcgcta aacaactggt tgaaaactat aaaggacgta tcttagcaga aagtgtagta
    16141 gggcaaggga caatttttag aatctttatt cctatcgcaa aggcagaaga caagtaaagc
    16201 atgtgcttta cttgtttttt tatatatcca acaaagaata aggtacaaaa tacaagcaag
    16261 ttagaaattc agggatgtca ttataaaaaa aactagcgaa caattaaata gaataacatc
    16321 cgttaattaa acgtaagtag cttttagatg aacgaaaaaa caatgaaatg ctgtaatatt
    16381 cgtgtttaaa aataataaat aaaaataggg aattttctat tgcaatttta taaagagatt
    16441 gctatattat tatttgttct aaatgattcc tttaaggtgt cactaaaaat tataaaaaat
    16501 agtgttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat gagatagcta
    16561 ataaagcttg attttatcga aaaaaacttc gaaaaagtta ttgacaaata acaataaaga
    16621 tggtatgata atcaagtcgc tgctaatgag tggcaaagac gtttagaaaa aataaacaaa
    16681 gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttaa aacaaaaaga
//
LOCUS       DANJMA010000053        16006 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000053 DANJMA010000000
VERSION     DANJMA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(39..1190)
                     /gene="queG"
                     /locus_tag="PWU31_002117"
     CDS             complement(39..1190)
                     /gene="queG"
                     /locus_tag="PWU31_002117"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL1084615.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1209..1961)
                     /locus_tag="PWU31_002118"
     CDS             complement(1209..1961)
                     /locus_tag="PWU31_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084616.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIHFVQLKKEKLYYGLYSR"
     gene            complement(2101..3051)
                     /locus_tag="PWU31_002119"
     CDS             complement(2101..3051)
                     /locus_tag="PWU31_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL1084617.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(3052..3891)
                     /locus_tag="PWU31_002120"
     CDS             complement(3052..3891)
                     /locus_tag="PWU31_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1084618.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(3888..4634)
                     /locus_tag="PWU31_002121"
     CDS             complement(3888..4634)
                     /locus_tag="PWU31_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1084619.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            4872..6242
                     /locus_tag="PWU31_002122"
     CDS             4872..6242
                     /locus_tag="PWU31_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1084620.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6297..7193)
                     /locus_tag="PWU31_002123"
     CDS             complement(6297..7193)
                     /locus_tag="PWU31_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1084621.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7307..7477)
                     /locus_tag="PWU31_002124"
     CDS             complement(7307..7477)
                     /locus_tag="PWU31_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084622.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(7474..8151)
                     /locus_tag="PWU31_002125"
     CDS             complement(7474..8151)
                     /locus_tag="PWU31_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1084623.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8242..9147)
                     /locus_tag="PWU31_002126"
     CDS             complement(8242..9147)
                     /locus_tag="PWU31_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1084624.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9322..10776
                     /locus_tag="PWU31_002127"
     CDS             9322..10776
                     /locus_tag="PWU31_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1084625.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVDPSH"
     gene            10792..11823
                     /locus_tag="PWU31_002128"
     CDS             10792..11823
                     /locus_tag="PWU31_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1084626.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11830..13017
                     /locus_tag="PWU31_002129"
     CDS             11830..13017
                     /locus_tag="PWU31_002129"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL1084627.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13173..14555
                     /locus_tag="PWU31_002130"
     CDS             13173..14555
                     /locus_tag="PWU31_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL1084628.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14527..14913
                     /locus_tag="PWU31_002131"
     CDS             14527..14913
                     /locus_tag="PWU31_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084629.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15113..15781)
                     /locus_tag="PWU31_002132"
     CDS             complement(15113..15781)
                     /locus_tag="PWU31_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL1084630.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         4585 a         3227 c         3004 g         5190 t
ORIGIN      
        1 ttactggaca aaaactttga ataatgttaa ctattttttt attttaactt tctccgcaat
       61 aatttctggg catgttccaa ttcgtttata acgacttctt ctgtttcttt agctaatatt
      121 tcatctaata catcaagcca tttttccggt tcttttgttc ctagacgtcc aatagcccaa
      181 gctgctgttc ctctgatgac agggcggaca tctgttaaac attctgaaat ctcaggcaat
      241 gccgttcgat cacctagatt agctaaagca atcaatgcgt ttctctgcaa aggtttcttc
      301 ccacgccatg aaccagctaa atgcccaaat tgctgtttga agtctttatt agacatcgaa
      361 agcattggct ttaattttgg atatacttcg tctacttttg gttccatctc ttcatgaaaa
      421 tgaaaatctt ttccttgatt gtaagggcag actaactggc agatatcaca tccataaatg
      481 acatttcgca tttttttacg atattcttcc ggcatcatcc cttttgtctg agtttgataa
      541 gacaaacatc gctgggcatt cattcttccg tcaccaagca atgcttttgt cggacaagca
      601 gtcacgcagc gcatacagtc accgcaacca aattctcctg gtttatcagg ctcaaataca
      661 atattagtaa cgatctcacc aagatagaca aatgaaccga actcttcagt gatcaacaat
      721 ccgtttcgtc caataaatcc taaaccagct cgctgtgcta cagcgacatc gatcagttct
      781 ccagtatcta cttgcggagc tagacgccat tcctcttctt tttgccattt ttctgctctt
      841 gtttggatga attcgatcaa tcggttcaaa cggtctctga gtatatcatg gtaatcgatc
      901 ccccaagaag cacgagcaaa ctgcccgcgt ttttcatccc taggcacttc ttcatggatc
      961 ctagtaggat acgcaagtgc aatggaaatg atcgatttcg gctgatcaaa tgtcatttct
     1021 ggataaagac gctcatcgat atttggatgt tcaaagccag aagtatggcc agctgctttt
     1081 tgttcttcca acgattcttt taatgaatca aaaggttccg ccgtggtgaa cccgattttg
     1141 tcaattccta atcgtttgct ttcttgtatg atttcttctt ttaatgtcat ggtcttcctc
     1201 gcttactgtt aacggctgta taacccataa tatagttttt cttttttcag ctggacaaaa
     1261 tggatctgct gatcggcaat cttcttttct tccatccatt tttttcttgc agcaaatact
     1321 tcccaataat ttgttgaaat taattttctt ttgttatcta atgcattaaa aaaattcaat
     1381 gaaactggtt ttgtacttaa cagttcatgg agccttacct cttcccatgg cgaaaactca
     1441 gacagatcgc gggaatacaa tacttcttca tagagatttt tttgctgtgt gtacgtgaat
     1501 gtttgcggga ttttcccagg cctggcaatt tctactagac gattataaaa catattcatt
     1561 aatttgctgt tggcaataaa ttgactttta taataggccg actggatctt tttgattact
     1621 ccttctgctt tgatgactga aaataatgca ggccatgctt tgacggttat ttcgtcgttt
     1681 tgtttaacta atacgccagg tgtgaagccg actggagaaa ggaacacaaa ataaccgata
     1741 ccggtatttt catctaaagg ctgaaattca tcaaacaacc atttgcctat gtacagctct
     1801 tcatctggtt ctgttgtctc attttgattt gctatgaata atttagctaa ttctgcttca
     1861 agaaattgaa ggtcttgatc cttgtcggta ttttcctgct ggctgatcaa tcgaaagact
     1921 tcttctttaa tcgtatattt atctttacgt gtaaaattca tagccacacc ttccttcctt
     1981 actctaacta taacggaatt tcttcaaaaa aggtatctgt acgccgaaaa acagcataat
     2041 ttttttcata tctcttataa aaaatgcaag gcctgctcct caagctagac cttgcacttt
     2101 tcagctttct tttatttgtg acaaaccttc atggatcgtt tcaatattcc atttcatcat
     2161 ctgatagtaa gaatctcctt gttctccttc ttttgctatg gaatcagtga acagtttatc
     2221 ataaattttc aaacccgttt cttttgctac ccgttccatg cttcttgagt ctacactggt
     2281 ttccacgaat aaaaccggta cttcttttgc tcttatctga tcaatgatcg ctttcatttg
     2341 atcaggcgta ccttgacttt ctgtattgat ctcccatata taagctgctg gcaaatcata
     2401 tgctcgtgaa aaatacttga atgccccttc acttgttact aataattttt tgttctcttc
     2461 gatagaagca aaactttcct tcgcttgtgt atctaattct tttagttttt ccacatatgc
     2521 ttctgcattt tttttataga gtgtcgcatt ttctgcatct ttttcagaga atatacgtgc
     2581 gatttcctcc acatatttta ttccgttaga taggtctagc caagcatggg gatctgcttt
     2641 tgtatgttct tcaccgctag ttaaataaag aggttctaca tttttgctaa ctgcaaaata
     2701 atcttttcct tcttcttttt tagccgtttc catcaagtta tcgaaccagc tgttacctgt
     2761 ttcaagattc aaaccgttgt ataaaataac atccgcatcg cttgcctttt tgatgtcttc
     2821 tggtagtact tcatattcat gcggatctgt tccgactggt acgatactgt ggatatctat
     2881 ttgatctgtt ccgacctctt ttgccatgtc cgccaagatc gaattggtag ccacgactga
     2941 caacttttct ttacttcctt catccgcttg gtcagaagcc tgctttccgc aaccggctaa
     3001 taccaagaaa ctaactgcca agattaaaaa gagtgatttt tttgttttca tctatctgct
     3061 tcctttctga aataaaattc cttttgttgg agaaaataaa aaagcaagga taaaaaatgc
     3121 ggtacatgct aacacgatcg tagctcctga tggccaattg taactataac tgaaaaacac
     3181 accgacaact gcacttagca taccgattcc tgcagctgta atgatcattt tcagtaaatg
     3241 gttcgttagc aagtaagccg tggcggctgg tgtgatcaac atggcaatca ccaagattgt
     3301 tcctactgtc tgtaaactga ctacagccac taatgtcaaa aagaacatca aaagataatg
     3361 gatcagcgat gtgttcagcc cataagcttt tgccattgtc ggatcgaatg atgtgatctt
     3421 caattcttta taaaagaaaa agacaaatat caagacgatt ccactaacga gtgctgtcag
     3481 caacaaatcg ctttccctaa cagctaagac attcccaaat aaaatatgat acaaatctgt
     3541 cgcactctgg gcatatgaaa tcaagataat ccctaatgca aaaaaggagc taaagacaat
     3601 tccaatcgct gtatcatttt tcaatcggct ccgctgagtc acgaaaccaa tcactgctgc
     3661 tgttagcatc ccaaaagcag tggctccaaa gatgtaacta aaaccaaaca tataagaaat
     3721 ggctacacct ggaagaactg cgtgagagat agcgtctccc atcaaagaca tccctcgtaa
     3781 aataataaaa gaaccgatga ctccagaagc aagtcctaca agcatggaag tgaccaaagc
     3841 gtattgcagg aattgataat tcattaatcc atcaataaag gattggatca ttttttctct
     3901 cctcctttta ctaaatgtct gatgatctca ccataggcca attgaaggtt cttcgatgta
     3961 aagacttctt gtactggacc ggaagcgatc agctgtttat ttaaaagaat aacctcatcg
     4021 aaatattctt ctactttatg aagatcatga tggacgatca aaatcgtttt tcctgaattc
     4081 ttcaattcct gcaagatgtc aaagatcttt cgttcactta acgcatcaat ccctacgaat
     4141 ggttcatcta aaaagatcca ttctgctcct tgggctagag ctcttgcaat aaaaactctc
     4201 tgaagctgtc cacccgatag ttcgctgatc tgtcttttgg catattcttc catccctact
     4261 tttttcaaag cttgtcttgc acgttctttt tcttttttcc caggtctttg tccaattcgt
     4321 aaagatggat atgttcctaa aagtacaacg cctaatacat caattggaaa ggaaagatcc
     4381 aattcacttc gctgttccac gtaggctatt tttttacgga ctgagtttac cggctgtcca
     4441 gaaaaggtga ctttaccagt agtagctggt atcaactcta gcaaagattt cataaatgtt
     4501 gattttcctg caccgtttgg tccgataata ccggtaattt tttgtggatt tatcgtcaag
     4561 ttgatagttt tcaaaacagt tttcccttga taagcggctg aaaggtcttg aattacaatg
     4621 gccgtacgtt ccaaatctta cactcctttt taggtttacc taacttttta tttaaatata
     4681 gtttaactaa aatggaaagc attttcaagt agaatgatga aaaagttttt ttcgaatttt
     4741 cctcgcattt tatgaaaatt ttctgtagaa tcacatcaag agttcaataa ggctaaccta
     4801 ataatgaaaa acatgtgttg aaacgtttga ttattttttc ttgttgactc aacaacgaaa
     4861 ggagtttttg tatggaagaa gaaaagttaa ctcgaagtct tagtgcccgt catatccaaa
     4921 tgatcgcact aggtggaacg atcggtgtcg gtctgtttat gggtgcctcc tcaaccatac
     4981 gttggacagg gccctcagtg atgggagcct atgcgattgc aggattcttt ttgtatttga
     5041 ttatgcgtgc tttaggcgaa atgctttacg ttgatccttc gactggttct tttgcaaatt
     5101 acgcgagcga gtacatccat cctgtggctg gctacttgac cgcttggagt aatatttttc
     5161 aatatattgt cgtaggaata agtgaagtga ttgctgtagg atcttatatg aattattggt
     5221 ggcctgattt gccggcaatt attcccggaa tcattgtcgt cttgtttctt atgctggcaa
     5281 atcttatttc tgtcaaggcg tttggcgaat tagaattttg gttttcgatc atcaaagtca
     5341 taacgatcat tttgatgatc attgcaggtc ttggggtaat cttatttgga tttggtaatc
     5401 atggtcaggc agtagggatt tcaaatcttt ggaaaaatgg tggattcttc actggaggcg
     5461 tcaaaggatt tttctttgca ttatcgatcg ttgttgcttc ctatcaagga atcgaactga
     5521 tcggaatgac tgctggtgaa gcagagaatc ctaaaaagac gatcattgaa gctgtccagt
     5581 ccactatcgg tcgaatcttg attttttata ttggtgctat ttttatcatt gtgagtatct
     5641 atccgtggaa tcaattgagt gagattggtt caccttttgt acaaactttt tctaaagtcg
     5701 gcattacatt tgctgcagga ctgattaatt ttgtcgtgat tacagcggca ttatcaggtt
     5761 gcaattcagg gattttcagc gcaagtcgta tggtctatac acttgccata aatggaaaga
     5821 tgtctaaaaa atttctgaaa ctatctcgtc acggtgcgcc tttttatcct gtcgtagcaa
     5881 tctcaggtgg aatcttaatt ggtctgatct taaattatct tctaccaatg ttcatcaacc
     5941 agtcagaaaa tattttcgtt ttcgtataca gctcaagtat tttgcctggt atggttcctt
     6001 gggtagtgat tttgatcagc gagattcgct tcaaaaagaa gcacgcagat aaaatgcatg
     6061 atcatccttt taaaatgcca ctatctcctt atacgaatta tgtgacactt gcttttttag
     6121 ctatcgtatt gattttcatg tttatcaatc ctgaaacgag aatctcattg attattggac
     6181 tcgttttctt gctttacatg acgatccatt actttgtacg cgaaaaaaga accggaaaat
     6241 aaaaaaagaa cgaagcttca ccttagtgga agcttcgttc ttttttattg cttttatcaa
     6301 ttcaatttta gcgtctcacc gggagtcagg aaataattct ccatgctcat cccattcagc
     6361 gacaagatcg tttctactgg tacgccagtt cgttcggcta tttgatttgg accttctcct
     6421 tccaaaacta cggttgtcgc agcctgtgaa gtcgcgctgt tggtattagt ttcagaggaa
     6481 tctgttgtgt cttgtgttgt tcgagcttgc atttcagaag cttctaccga ctctgctgtc
     6541 tcagaatcag aagatgttgt gtcttgtgtg cttgttgact ctgcttgaag agaggcgcta
     6601 gtctgcaaat aaatatcgga aatcttagcg acatgattcg tataattagc cgataattga
     6661 ttgatcaaag tatctaagtc tacaccgtct ttattttctg ctgcctgata cgctgcacgt
     6721 aattttctga tcccttcgtc ggagattgct tgaagctcac gtgctttttg gttagcaact
     6781 gctgataaac cagctattcc ctcttgattt ccctgaattt ctgtctggta ttcttcaatc
     6841 aatgcaggcg tcttttcttc gatctttttc gaataatcat ttacgattgc ttgaagttgc
     6901 tctggcaaac catttgctgt tttttcttca gaagtttgtg tctcttttgt tttagttgat
     6961 gactcacttg tttgtttcgt ctctgttttc tttgtcgttg tttccgttat ggtcgtactt
     7021 ttcgcttctg acgaattttt tccatgaaaa tatagacccg caactcctcc tgctatgatc
     7081 aaaaacacgg caactgctgc cccaatcatg atcatccgct tacgagctgc ctgtttttgc
     7141 tgtcttcttc gggtcatccg aacattttct ccccactcat tttcatcata catttttttc
     7201 accaccaatt tttatgtata atagcgttat caaacgcttc atattcttat tgtagcaaat
     7261 aattgtcgct gtgcacattt ttatctttgt tatttataag aaaatttcac cagttgagat
     7321 gattccaaga ttgtccgtcg atttctcctt gcagctgcat tgttcgttcg tctatctcag
     7381 ccatcagttt ctttatttca tataagagcg cacggtcttg atattctgat ttttcgctta
     7441 attcatctaa ttgttggatc agccaatcat ttgtcataat gaagttcctt tcatttcttg
     7501 cagtgtaaag gtcaagtctt cgatctgctc ttttgcctca gtcagtgctt ctacgatata
     7561 tgcttgtttt tcagattctt cttcctgcat tttctgattt tcctgtactc tttgaataaa
     7621 ccagtcaatc cgctgagtca gcgatggttt gccttccaat gagagatact gctgataagc
     7681 aattagcttc tcccaacgtt tttcctggga taaatacata aattgtgcta aaacgaatgg
     7741 cggaagatag gtcatatgga atttatttgc gattcgctga tacggcctca aaaattcgga
     7801 aatcgtatat tcttcttttc ctccagcttg gtattctttt tccgccacac ctgttgtcaa
     7861 tacgattcct agttctttcc cttttaatac atgtgctgct cgttccaaga cattatcttg
     7921 ccaaattttt aaatgagcag gagaactata ccagtaaaac ggaaactgga aaatgatccg
     7981 atcatgctgg gccaagagct gtagttcagt ctctatatct atttttccgt ctggataact
     8041 tgattccaaa tgatgccaag tagctggtaa accagcagaa gcttctttga aaaattgctg
     8101 atttaaagat tcttcgatcg ttggatggct aatgatgata agtgtacgca tcccttttcc
     8161 tcctttttag tcttcgcttt acagtgtagc ataaccgcca caagtttctt ttgccgatgc
     8221 ttgtcatttt aaccagacta atcaggttgt ccaagaaact gcggtttgtt tttccaactg
     8281 ttttttcaag gtatccaaag caatattctt aaaattttcg tttttcatac gtttacgata
     8341 gttcgtagcc gtttctccgg tactcttttt gaacattcgg ctaaatacga ctagactatt
     8401 gaaccctact tgataggcaa tactttccac ggattcatcc gtcagagaaa gaagaaacgc
     8461 ccctcgattg atccgtaccc cgatcaaata ttccttgata ctggtaccca tatgattttt
     8521 gaatactcgc gacaaatggc tccgattgac cgctaaatgc tgggcgattt cctcaacaga
     8581 caatttttca gcataatgtg cctcgatgta tttgactgtt tcaacagcta aagaggaatg
     8641 aaccttttgt acaccaccgc tgacctttcc gccatctttt aaaaggactg ataaaaaacg
     8701 gtaggttaat gctgttaact gtaattcatt ttccaaggtt tcttccgtat ctttcatgca
     8761 ttctaaaata atgtcgatat atttttgtat gtcagaagag accatcgtgt attggtcttc
     8821 tctgaaaagc gactgccgaa tgatttcttc tgcagcactc ccaccaaatg aaagccagca
     8881 atacatccac ggatcttctc catctgataa gtaaaacgtc gagtctcctg gacgaatcaa
     8941 gaacagatca ccttttttca attggaattg ttgatttttc acatgataag atcctttgcc
     9001 ttccatcacg acatgcagga tgtaatcatt gcgaatagca ggaccaaaag aatgaaacgg
     9061 catcgtttta gagaaaccac aaaaatcaaa atacaattct ttttctactt gttgatgttc
     9121 cttttttaaa aaaatcgatt gctccacgtc tatccctcct tactccttta gtataatgca
     9181 acaaaaagtt aaataaaagt cacagaaata tattaaaaag gaagcgattt cttgttaagt
     9241 ttaagtcatt aaaagatctt tgttattcgt tgtaaccggt ttacttagta ttatatattg
     9301 aacaggagag gaatacagat tatgaaaagc aaaatgatcc aacgtgtgtc ctacgcttta
     9361 ggcgcttttg gacacgacac attctatgtt acattaagca cttattttat ggtattcgtg
     9421 acaagccaga tgttcgcagg aacagataag gcaacgaatg caaaaatgat tgctacagtt
     9481 acaagcttag ttgtaggtat ccgcttagtt gaaattattt ttgatccttt gattggtggc
     9541 atcatcgata atactcgtac aaaatacggt aaattcaaac catggttagt tatcggcggc
     9601 gttgttagtt ccgtactctt ggtagcaatc tttaccaatt tctttggctt agctactcat
     9661 aatacgactt tatttatcat attatttgca atcgttttca tcttattgga tagtttttat
     9721 tcatttaagg atatcgcttt ttggtcaatg attccagctt tgagtacgga aacaaaggaa
     9781 cgtgaaaaat ttgctacgtt tgctcgtttc ggctcttctc taggtgcaaa tggtactact
     9841 ctagtcgttg tccctatcgt tactttcttt acctatcttg ttactcataa acatattgaa
     9901 ggcgcttcag gctggttctg gttcggtgtg attgctgctg ttatttcagg cggtactgcg
     9961 attatcactg ccttgggcac aaaagaagtc gatactgcga tccgtcagca aaatgaaaaa
    10021 gctgggatca aagatgtgtt caaagcagtt gctcaaaatg atcagttgat gtggcttgcc
    10081 cttgcttata tcgcttatgc gattgccaat gcagcaacaa ctgccgtatt attctacttc
    10141 ttcaaatatg tacttggcca accaactgaa ttttggatcg ttggtctagt tgctgcctta
    10201 cttggtttag tagctgttcc actattccca atgcttacac gcgtcatctc tcgtcgctac
    10261 gtttaccttg gagggatcgc aatgatgatt tcttcttact ttttctttac gatcgcaggt
    10321 tcaagtttgc caatcgttat catcgggcta gtattgttct acttccctca acaattgatt
    10381 ttcttatccg ctttgatgac gatcacagac tctgttgaat atggtcaatg gaaaaatggt
    10441 atccgtaacg aagctgttac tctatcaatt cgccctttac tagataaaat cgctggtgct
    10501 ttgtctaacg ggatcgtagg gtttgtcgct atcgcttctg ggatgactgg taatgctact
    10561 gctgctgata tcactgctca tggcgtaacg actttcaaag cttatgcatt ctatgctcct
    10621 gctgctttga tgattttctc cgctattatt tttgcttgga agattacatt gacagagaaa
    10681 aaacatgcac aaatcgttga ggaattggaa aacaaattgg ctccagctga tacaatggaa
    10741 gatgaacttc acgatgcagt agatccttcc cattaagaaa ggatcttgaa aatgaagatt
    10801 acagaaaaag attttggcca aggttatcac ttgataacct tggagaacaa aaacaagcta
    10861 gcactttcta tctctgattt aggtgctaga atcgtcagtt taaaatcaaa tgatcgagaa
    10921 ttagttttgg gatttgacac cgctgaagaa tatatcgaaa aagatccgta tatcggtgct
    10981 tctatcggtc gcaccgcagg gagaatcgaa aatggccgct tttctctcaa tggaaaaact
    11041 taccaattag ccacagatcc aaaaactgga cataatctgc atggtggtgc tcctggattt
    11101 gaattaaaaa aatggtctta tgtcattttg aatggcgaaa atgaagcttc tgtcattttt
    11161 actactacaa gtcccgacgg agaacatggt tttccaggta caatggatgt ggaaatccgc
    11221 tatactttga caaaagataa tatctggcga gtgacctctc gcggcaccag tgaccaagat
    11281 actttattca atccgacaaa tcatgtttac ttcaacttga cgggagatgc ttctcagtca
    11341 atcgatcaac atgagctttg gctcaatagc gaagcttatg ctcctttgcg aacagatagt
    11401 attcctatag gggtaaaaga aaatgctgca ggaagtgctt tcgattttca aatacctaaa
    11461 aaattagcgt ccgtttttgc tagtgatttg gatcaaaaaa atcttgttga cggaatcgat
    11521 catccatttt tcttaaaaga aaccggcttg ggtaaagaag ccgctcgctt gactagcccc
    11581 gataaaagaa tccaagtagg tattgctaca gatgcttcta gtgtggttat tttcactgca
    11641 aatttcggca cagaaacacc agaaatgcgc aaccgaaaac tggcacatca cggtggcatc
    11701 acatttgaga ctcaaacagc tccaggtgca gagcgcttct cagcatttgg ttcgattcat
    11761 ttaaaagccg gatcagtatt tgaaacagtg acagaattca aaatcaaaac aagaaaggaa
    11821 taactcatca tggaaatcaa aagaagctta aacacaaaat ttgccgaaac attcggacct
    11881 aaaaaaacta cacaatactt ctcaccagga cgcatcaatt tgattggtga acacacagat
    11941 tacaatggag gccatgtctt ccccgcttcc atcacttatg gaacgcaagg tgtagcagca
    12001 cctcgagacg ataataaaat attagtttat tccacaaact ttgacgatat cggtgtcatt
    12061 agttttactt tagatgagct agaatatgat aaaaaagcaa actgggcaaa ttacgtaaaa
    12121 ggaatggtcc ttaaattaaa agaagctgga cactcaatcg atcatgggtt tgagttgtta
    12181 gtggaaggaa caatccctaa tggtgcagga ctttccagta gcgcttcatt agaattatta
    12241 gtaggtgttg tattagaaga tctgtttgat ttaaaaacag atcgcttgga actagtccaa
    12301 acaggtaaaa aagtagaaaa tgaattcatc ggtgtcaatt caggtatcat ggatcagttt
    12361 gccatcggct tcggtgagct agatcatgca atcttattag atacaaatac gctgaaatac
    12421 gaaatggttc cagtgaaact agatggttat gcaatcgtca tcatgaatac gaataagcgg
    12481 cgggaattgg cagattcaaa atacaatgaa cgccgtagcg aatgcgaaga agccttggca
    12541 cgtttgcaaa aaactttgcc tatctcttct ttaggagact tagatgaaga agaatttgaa
    12601 tcaactattg accaaatcgg cgatgataca ttgatacgca gagctcgcca tgccgtttct
    12661 gaaaatcagc ggactttgaa ggcaaaagct gaattagaag ctgggaactt agaagcattt
    12721 ggtcaactgt taaatgattc tcaccactct cttcgctatg attatgaagt aactgggatc
    12781 gaattagata cactagtaga tgctgctcaa aaacaagaag gtgtcttagg tgcacgaatg
    12841 acaggagctg gatttggtgg ctgtgcgatt gctcttgtca aagaagaaaa tatacctgcc
    12901 ttcaaaaaca atgtctatga tgaatacatg aaagttgtcg gctatgctcc ggaattttat
    12961 gttgcacaca ttggaaatgg gacaacaaag atcgatccag aagctgttca tgtttgataa
    13021 aaaaatcaac gatctcagct aaaaacaaat tgataaaaat aacctctcag caatttccaa
    13081 atgtgagcct tttgctcgta tttgaaaatt gctgtttttt attgacacgg aaagagaacg
    13141 tgtgttctaa ttgtgttgaa gaggtgattg ttatgtgggc gaatcaaaat ccggtatttg
    13201 tttatgaaaa agaaccttca agagatattt tatgtattga ctgcaaatct ttttacgcct
    13261 ccgtagagtg cgttgaaaag gatcttgatc cccttacaac taaattagta gtcatgtcct
    13321 atccgtccga ttcgacagaa acacggggta gcggattaat cttggcttcc agtcctgcag
    13381 caaaaaaagc ttacggcatc accaatatca gccgtgccag agatcttccc ttcccctatc
    13441 caagtgattt atatattgtt gccccaagga tggcctatta tatggaaaag aacagagaaa
    13501 tcaattctat ctataaaaaa tatgcagacg aagcgaacca tcatgtctat tccgtcgatg
    13561 aaagcttcct tgatgtaaca aatagcatgg cactctttgg taaaacaaat gcgtacgaca
    13621 tggctaaact gatccataaa gatgtgtttg agcagaccgg aatctacacg actataggaa
    13681 ttggcgataa cccgctactt gctaaactag ccttagacaa tggctcaaaa gacggaaagc
    13741 aaatgtttgc tgaatggcgt tatgaagatg taccagaaaa gttatggaaa atctcagata
    13801 tcactgactt ttggggaatc ggcaaacgaa cagcaaaacg tctgaaacaa atgggtatac
    13861 attctgttta tgatttagct cacgcggatt attatcactt gaaaagcaag ctaggggtca
    13921 tcggtactca gctttatgcc catgcttggg gcatcgatcg aagttttttg ggacaagaat
    13981 atacaccaaa atcaaaaagt atcggaaata gtcaaatcct ggatagagat tatacaagac
    14041 gagaagaaat cgagatcgtt atcaaagaaa tggccgacca agtcggcact cgcttgagac
    14101 gatctgcagc aaaaacacaa gtagtcagct tatgggtagg atactcttta ggctatcttg
    14161 atccaaatgg caagaccggg tttcaccagc aaatgaaaat cgaagcgaca aatgctagca
    14221 gagatctagc agatgcttta ttgaccattt tccaccagca ttaccactac caggatattc
    14281 ggcgtgtagg cgtgaactgt tccgacctcg tatacgcgac aggtttacag cttaaccttt
    14341 ttgaagatcc tgaaaagcag ataaatgaag cgaaagttga ttttgttgtc gatacgatta
    14401 gaaaaaaata cggctttcaa tcaatcatcc atgcgcatag tctcttgcaa ggaggacgcg
    14461 ccatcgcacg tagttctctc gttggcgggc atgcaggtgg tatgagtgga ttagaaggtg
    14521 gaaaagatga gacggacagc aaaaaaattc attgactata acgagtatcg agatcggcca
    14581 tttggattaa aatgggggac tgcttttgcg atggaagaac tagtcaacgg tatcaaaaaa
    14641 aatgaagaag aagcacgcaa acagcctctt cctaaacaac aaatgacatt gcaagaaatc
    14701 gatagtatac tgacagaagc ctttttatct cgtaaagcag taatcattca gctcaattta
    14761 aaagatgaat ttggaagact cttggatgag gtcgaagggc acttcattgg tgaagcctat
    14821 gaagattact ttatgattga ccatcagccg atattgtggg aagacgtacg gtatattgag
    14881 ttaaaggaaa caaaaaaatg gtttgtgaca tgagaggttg tgacagaagt gatcaacttc
    14941 aaaaaataag aaaggatttt tcgaaaattg cttcttaaat ttttgtggaa ttttggctta
    15001 tttttcgaag aagcaacttc tggaacaacg tatattagat atcagaggtt gtgaaaaagt
    15061 cgcttagttt tgagtaacaa gccaaggcgc tgatttcaca acctctgtct atctatccat
    15121 ctattcggta agattcatgc atgacgtctt ctttatttag tatgtagagc cctttttctt
    15181 ctgaccattc aacataaatc agctgtttta catctgcata gccttctttc ttcaagttct
    15241 ccataagcca tgcttcagtc aattgattgg actgaagcac tttatgctga atttgccctt
    15301 catctacaag caggatcgat aaagctttgt cttcatctga ttttttcaat acactcattt
    15361 gaccggcatt ctctaggata acatgtttgg cattttcaag agaaaagcat tcttgctgtc
    15421 ttaagattgc tcgcaactgc tccatctcga tatggttatt tgtcaactct gtcatgttga
    15481 tgacgccgtc tttgattaga acagatggct cgcctttgac ccaacgattg accttttcat
    15541 ttttttggac gatacgttcg ataccataaa tcattactgc ccaaagcgca atggcaaata
    15601 ggacatgccc tacatggaca ttgtcatcat aggttgattc ttctaagatc ccacctaaaa
    15661 caagcgtata gactaaatca aacggtgtga tttctgacat ggatttcttc ccaagcaaac
    15721 gtaccactaa taataaaccg atcaacccga acaccagttt cattgtaatt gaaaaaatca
    15781 ttttttctcc ccctaactat ctgttaatta aattatatat gctttctcac gaacacgcat
    15841 ggaatataca aggaagaaat gtatcctttt actaaaaaaa ggactgtgac actgtagtga
    15901 cccccaaaag ttagactttt ttggagtgga gaaatccact cctttttttt atgctctaaa
    15961 atgatatttt tacacaaccg ttacggttgt tttacgaaat tggacg
//
LOCUS       DANJMA010000054        15453 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000054 DANJMA010000000
VERSION     DANJMA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(193..378)
                     /locus_tag="PWU31_002133"
     CDS             complement(193..378)
                     /locus_tag="PWU31_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084631.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1228..1569)
                     /locus_tag="PWU31_002134"
     CDS             complement(1228..1569)
                     /locus_tag="PWU31_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1084632.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1569..1799)
                     /locus_tag="PWU31_002135"
     CDS             complement(1569..1799)
                     /locus_tag="PWU31_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1084633.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2095..2433
                     /locus_tag="PWU31_002136"
     CDS             2095..2433
                     /locus_tag="PWU31_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084634.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2638..3213)
                     /locus_tag="PWU31_002137"
     CDS             complement(2638..3213)
                     /locus_tag="PWU31_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL1084635.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(3578..4159)
                     /locus_tag="PWU31_002138"
     CDS             complement(3578..4159)
                     /locus_tag="PWU31_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1084636.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDAEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4342..4968)
                     /locus_tag="PWU31_002139"
     CDS             complement(4342..4968)
                     /locus_tag="PWU31_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1084637.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4990..6318)
                     /locus_tag="PWU31_002140"
     CDS             complement(4990..6318)
                     /locus_tag="PWU31_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1084638.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERFFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6476..6877)
                     /locus_tag="PWU31_002141"
     CDS             complement(6476..6877)
                     /locus_tag="PWU31_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1084639.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAEMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6963..7169)
                     /locus_tag="PWU31_002142"
     CDS             complement(6963..7169)
                     /locus_tag="PWU31_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084640.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7264..8766)
                     /locus_tag="PWU31_002143"
     CDS             complement(7264..8766)
                     /locus_tag="PWU31_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL1084641.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTNPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTRYAQDLVTKIKKGMGTQDDKPLKGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8784..9239)
                     /gene="argR"
                     /locus_tag="PWU31_002144"
     CDS             complement(8784..9239)
                     /gene="argR"
                     /locus_tag="PWU31_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL1084642.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9337..9954)
                     /locus_tag="PWU31_002145"
     CDS             complement(9337..9954)
                     /locus_tag="PWU31_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL1084643.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYDEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10175..11548
                     /locus_tag="PWU31_002146"
     CDS             10175..11548
                     /locus_tag="PWU31_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1084644.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11623..12735
                     /locus_tag="PWU31_002147"
     CDS             11623..12735
                     /locus_tag="PWU31_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL1084645.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12807..14444)
                     /locus_tag="PWU31_002148"
     CDS             complement(12807..14444)
                     /locus_tag="PWU31_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL1084646.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14612..15220)
                     /locus_tag="PWU31_002149"
     CDS             complement(14612..15220)
                     /locus_tag="PWU31_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1084647.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPVAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DATYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4297 a         3141 c         2688 g         5327 t
ORIGIN      
        1 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
       61 ctatcatttt tatcctcaac tacatacata tctgttgcaa cttcactttc tgtctcatcc
      121 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
      181 ccaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
      241 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
      301 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
      361 tttagaattt ttactcattc ataaacactt ccttaaatag cttttattat agaaggtatt
      421 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaccttaca ttatactgga
      481 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
      541 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
      601 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
      661 gcacattttc gagcattaca agtattggtg tcgttagaac aactaaatag aaactagcgg
      721 tagatttttc agaatctatg ctaatttctg tacggatgtt ccagtgatgg caaatttctc
      781 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
      841 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
      901 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
      961 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
     1021 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
     1081 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatccc atcgaccaca
     1141 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
     1201 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
     1261 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
     1321 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
     1381 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
     1441 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
     1501 tactgaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
     1561 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
     1621 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
     1681 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
     1741 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
     1801 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
     1861 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
     1921 aattgtagcc agttcatttt ttctttaaat aaaaaaataa tccaagaaac attgaaccta
     1981 tggatccaaa agctacccaa atactatagc taactccccg ctaccttcaa tcttgcagat
     2041 aaaacgcttt ggaaaaaaat cgagaaaatc gcaaagacga caggccatcc tatcatgcct
     2101 gattataaac aaaaagctgg ctattccttt tgctctgaca aatttcaatt gggaaagttc
     2161 acgaaactag caggtgaaga agtttcaact gttcggatta aagaatgccc gattcaaata
     2221 gaaacattgg ttcaacgaat ttcaactaga caagactttg ctattgtaga acgtgagatc
     2281 gtagctattt ttgtagaaga agggatttta catgatccaa ctcatatcaa tgtggataaa
     2341 tggcaacctc ttatctataa attcagagaa tatacaactg ccaaccagtc gttaactttg
     2401 aattttagat tccaagaatt caaagagtca taatgataaa catcgttagg tataaataaa
     2461 aaagactcgc agcacgagta aaaaacaaat agaagctata cattagaaag gtcgactgtg
     2521 accctataga tgcagtacat gcagcttatg agcatttctt ctgttctctt tgtttttggg
     2581 atctgtgctg aagtcttttt cgttttttgc cgctctttct ttgaaaaatc gattatgtta
     2641 taccttctcc attactaatt caggcatctg ctgagtaatg atttttcgcg caaaagaaat
     2701 atctgttacc caaggttcga tttgttcttc agtcaagaca agcggcattc ggtcatggat
     2761 tggtgcgact gtgcggttag gttcagtagt cattatgatt gattctgcca ttttttcatc
     2821 ctgatggcta cgataaaaac cgccaatata cactattttt tccttatttg aaaacataaa
     2881 tttgtctttt tcttcattcc actcataaaa gccactcatt gggaaaacac aacgattttg
     2941 caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg cattgatcat
     3001 caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag tgaggatgtt
     3061 ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc cagctgctaa
     3121 ttctgctgcc tgctccggtt gtttcttctg ggcttctcga taataattct gaaggagctc
     3181 tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaacc tttttttcta
     3241 tcatacactg caacaagaag ttcgtaccaa aaaaacgctt tcaatttgtg tagaactaaa
     3301 aaaattgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa ctccttggtc
     3361 acaagtcaca atattcaaca aacatgagaa gctatttttt gaaattgttc cttgtgaccc
     3421 gaagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttccagaagt agcttcttcg
     3481 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatg ccttcttatt
     3541 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
     3601 acaatctttc tttgatcaat tggaaattct gtctaagtag tgatagatct tgtgtgactt
     3661 tcaacaataa gatactgccc tggatttcag caatcaacgc tttggcttga cggtctgcct
     3721 ctgcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
     3781 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
     3841 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
     3901 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
     3961 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
     4021 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
     4081 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
     4141 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
     4201 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
     4261 acagccctca ttataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
     4321 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
     4381 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt gaccaggagc
     4441 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
     4501 ggaatcgcta cacaagtttc cgttcattcc accgagaatt actgtgtcga ttccattttt
     4561 tctcaattga agcgctaagt cgttgctttc tggtccaaag attttatgag gagagctgat
     4621 gatcgtattt tcatccaaat atggtttcaa ttcttcgatg aaatcagctc ctgaaccttc
     4681 ttcaactgat tcatattgac ttttcctagc aaacatctgc tgttcaagca tcatgtgttc
     4741 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
     4801 gagtttgtat cctttttctt ttgcttgttt caccatttcg atcatgtttt cgatcgtatt
     4861 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
     4921 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
     4981 attcgttatt tattttaata catcatttaa agcttcactc attgttggat gagaatagat
     5041 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
     5101 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
     5161 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
     5221 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
     5281 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
     5341 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
     5401 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
     5461 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
     5521 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
     5581 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
     5641 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
     5701 taaaatcatt tgcgcgatat cttcatcttc acgaggaaga aattcaggat gggcatcaag
     5761 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
     5821 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
     5881 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
     5941 attgatgaaa aaccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
     6001 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
     6061 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
     6121 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
     6181 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
     6241 gccttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
     6301 attttgatat ttttccatcc tttttccctc gcttcttttt tacctaccga ttggtacaaa
     6361 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
     6421 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
     6481 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
     6541 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
     6601 atactacacc gatctgatag ccaccatcat ctaaattata aagagagaca cgtgctttta
     6661 ctgtggactc tgctgtcatc tctgcttctt taatcatgtg ctgtactgca ccattgaaac
     6721 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
     6781 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
     6841 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
     6901 atttgctact tggctataac tatagtgtat gacaagtaag gcgctttcta caaaaattct
     6961 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
     7021 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
     7081 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
     7141 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
     7201 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
     7261 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
     7321 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
     7381 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
     7441 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
     7501 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
     7561 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
     7621 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
     7681 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
     7741 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
     7801 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
     7861 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
     7921 gatcaaatta tttcgattga tcactgttcc ggaagcactg acaactgcag aacgatccac
     7981 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
     8041 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
     8101 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
     8161 tccaccagca ccatttccag catccacgat gattttccaa ccttttagtg gtttgtcatc
     8221 ttgtgttccc atcccttttt tgatttttgt gactaaatct tgcgcataac gtgtcaaaag
     8281 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
     8341 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
     8401 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
     8461 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
     8521 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
     8581 atgaccaatc ccaatcgtcc attttttatt tggattggtt gacttcttct ttttctttat
     8641 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
     8701 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgtagcg ctttcatatc
     8761 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
     8821 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
     8881 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
     8941 tcagtcaata cgatgaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
     9001 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
     9061 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
     9121 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
     9181 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
     9241 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
     9301 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
     9361 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
     9421 gtgttcttta gaagctcttt catagccttc ttttgcgata aatgcttcta atttctcaaa
     9481 cgttgctggt tcttcgtcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
     9541 catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat
     9601 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
     9661 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
     9721 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
     9781 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
     9841 attcgggtcg ccgatccctc tgatgctaaa gaatggtttt ttaggaacct ctagtatttg
     9901 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
     9961 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
    10021 atcgtgttga cactattttt cagtttgtgc tagaataagt taagtaaata aatcgtacag
    10081 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
    10141 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
    10201 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
    10261 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
    10321 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
    10381 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
    10441 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
    10501 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
    10561 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
    10621 aattggtccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
    10681 gtttcttgtg cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat
    10741 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
    10801 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
    10861 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccgct
    10921 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
    10981 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
    11041 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
    11101 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
    11161 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    11221 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    11281 caatatgatg accaccggaa tgaatgcact gaatgaaaaa ctacaaggag acgggaacgc
    11341 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    11401 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    11461 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    11521 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gtaggtgttg catctgccgt
    11581 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    11641 atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat
    11701 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    11761 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    11821 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    11881 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    11941 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca aacatctcaa
    12001 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    12061 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    12121 caggcaaaag tcacttcgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    12181 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    12241 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    12301 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    12361 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    12421 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    12481 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    12541 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    12601 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    12661 ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg
    12721 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    12781 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    12841 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    12901 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    12961 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    13021 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    13081 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    13141 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    13201 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    13261 gttgcaccaa aagacgctgc taaaaccggg tcattgatct tgataattgg cggaagcgaa
    13321 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    13381 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    13441 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    13501 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    13561 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    13621 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    13681 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    13741 gcaataaaac cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    13801 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    13861 agtccattgg gaaatgtttc atgtgtccac tcgggatcac aaaagatata aagatctccc
    13921 tcatcaaagg aaacagactt ttgataagtc tttaagatct tcgaattcgc tggctgaaaa
    13981 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    14041 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgtttgcga
    14101 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    14161 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    14221 gtcctgacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    14281 atcgttcctg gactcccttt tgctagttga taagctgtct tcctgtcaaa aacttcatga
    14341 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    14401 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    14461 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    14521 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    14581 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    14641 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    14701 gattggcagc tgaatagtcg ccgtttaagt atgtagcatc taattgataa cggccgatgt
    14761 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    14821 cagagcttga agttggcgct gttgcagcaa ctggggcagc aggagctact tcaactgttt
    14881 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    14941 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    15001 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    15061 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    15121 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    15181 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc
    15241 cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag
    15301 aatcatgaca tttaatgaca aaatgacgaa ttatttaata tttgtaacat ttaaacaaat
    15361 atttttttga tacaaaacca taaaatcctt gttgtataag caaagtaatc gtttttctat
    15421 gtttttttat ttttgaatga aaaacgcaga ttc
//
LOCUS       DANJMA010000055        14325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000055 DANJMA010000000
VERSION     DANJMA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            249..2414
                     /gene="acm"
                     /locus_tag="PWU31_002150"
     CDS             249..2414
                     /gene="acm"
                     /locus_tag="PWU31_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL1084648.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2686..3171)
                     /locus_tag="PWU31_002151"
     CDS             complement(2686..3171)
                     /locus_tag="PWU31_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL1084649.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3261..3606
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3651..4292
                     /locus_tag="PWU31_002152"
     CDS             3651..4292
                     /locus_tag="PWU31_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1084650.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4311..4946
                     /locus_tag="PWU31_002153"
     CDS             4311..4946
                     /locus_tag="PWU31_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1084651.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4964..5779
                     /locus_tag="PWU31_002154"
     CDS             4964..5779
                     /locus_tag="PWU31_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1084652.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6098..7534
                     /locus_tag="PWU31_002155"
     CDS             6098..7534
                     /locus_tag="PWU31_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1084653.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7723..10170
                     /locus_tag="PWU31_002156"
     CDS             7723..10170
                     /locus_tag="PWU31_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL1084654.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10400..11467
                     /gene="rlmN"
                     /locus_tag="PWU31_002157"
     CDS             10400..11467
                     /gene="rlmN"
                     /locus_tag="PWU31_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL1084655.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11660..12763)
                     /locus_tag="PWU31_002158"
     CDS             complement(11660..12763)
                     /locus_tag="PWU31_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1084656.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13316..13864
                     /gene="aac(6')"
                     /locus_tag="PWU31_002159"
     CDS             13316..13864
                     /gene="aac(6')"
                     /locus_tag="PWU31_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL1084657.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4929 a         2351 c         3010 g         4035 t
ORIGIN      
        1 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
       61 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
      121 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
      181 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
      241 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
      301 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
      361 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
      421 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
      481 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
      541 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
      601 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
      661 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
      721 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
      781 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
      841 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
      901 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
      961 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
     1021 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
     1081 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
     1141 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
     1201 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
     1261 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
     1321 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
     1381 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
     1441 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
     1501 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
     1561 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
     1621 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
     1681 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
     1741 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
     1801 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
     1861 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
     1921 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
     1981 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
     2041 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
     2101 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
     2161 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
     2221 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
     2281 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
     2341 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
     2401 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
     2461 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
     2521 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
     2581 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
     2641 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
     2701 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
     2761 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
     2821 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
     2881 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
     2941 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
     3001 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
     3061 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
     3121 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
     3181 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
     3241 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
     3301 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
     3361 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
     3421 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
     3481 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
     3541 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
     3601 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
     3661 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
     3721 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
     3781 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
     3841 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
     3901 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
     3961 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
     4021 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
     4081 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
     4141 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
     4201 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
     4261 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
     4321 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
     4381 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
     4441 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
     4501 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
     4561 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
     4621 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
     4681 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
     4741 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
     4801 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
     4861 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
     4921 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
     4981 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
     5041 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
     5101 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
     5161 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
     5221 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
     5281 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
     5341 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
     5401 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
     5461 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
     5521 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
     5581 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
     5641 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
     5701 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
     5761 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
     5821 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
     5881 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
     5941 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
     6001 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
     6061 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
     6121 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
     6181 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
     6241 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
     6301 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
     6361 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
     6421 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
     6481 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
     6541 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
     6601 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
     6661 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
     6721 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
     6781 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
     6841 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
     6901 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
     6961 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
     7021 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
     7081 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
     7141 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
     7201 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
     7261 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
     7321 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
     7381 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
     7441 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
     7501 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
     7561 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
     7621 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
     7681 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
     7741 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
     7801 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
     7861 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
     7921 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
     7981 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
     8041 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
     8101 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
     8161 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
     8221 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
     8281 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
     8341 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
     8401 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
     8461 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
     8521 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
     8581 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
     8641 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
     8701 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
     8761 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
     8821 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
     8881 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
     8941 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
     9001 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
     9061 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
     9121 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
     9181 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
     9241 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
     9301 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
     9361 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
     9421 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
     9481 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
     9541 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
     9601 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
     9661 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
     9721 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
     9781 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
     9841 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
     9901 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
     9961 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    10021 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    10081 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    10141 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    10201 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    10261 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    10321 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    10381 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    10441 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    10501 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    10561 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    10621 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    10681 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    10741 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    10801 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    10861 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    10921 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    10981 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    11041 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    11101 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    11161 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    11221 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    11281 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    11341 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    11401 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    11461 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    11521 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    11581 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    11641 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    11701 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    11761 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    11821 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    11881 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    11941 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    12001 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    12061 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    12121 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    12181 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    12241 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    12301 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    12361 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    12421 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    12481 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    12541 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    12601 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    12661 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    12721 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    12781 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    12841 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    12901 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    12961 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    13021 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
    13081 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    13141 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
    13201 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    13261 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    13321 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    13381 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    13441 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    13501 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    13561 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    13621 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    13681 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    13741 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    13801 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    13861 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    13921 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    13981 caccgtgtac cagaggttgt gaaaaagtcg ctagtttcga gtagtaagaa aaattttgtg
    14041 aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    14101 caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat
    14161 tcttttttat ctttgtatgg caataataga caatataacg aaaattattt aatggctttt
    14221 ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca
    14281 cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgat
//
LOCUS       DANJMA010000056        14101 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000056 DANJMA010000000
VERSION     DANJMA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            309..668
                     /locus_tag="PWU31_002160"
                     /pseudo
     CDS             309..668
                     /locus_tag="PWU31_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(963..2096)
                     /locus_tag="PWU31_002161"
     CDS             complement(963..2096)
                     /locus_tag="PWU31_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1084658.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2087..2755)
                     /locus_tag="PWU31_002162"
     CDS             complement(2087..2755)
                     /locus_tag="PWU31_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084659.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3211..4197)
                     /gene="lacD"
                     /locus_tag="PWU31_002163"
     CDS             complement(3211..4197)
                     /gene="lacD"
                     /locus_tag="PWU31_002163"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL1084660.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4210..5136)
                     /gene="lacC"
                     /locus_tag="PWU31_002164"
     CDS             complement(4210..5136)
                     /gene="lacC"
                     /locus_tag="PWU31_002164"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL1084661.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5149..5664)
                     /gene="lacB"
                     /locus_tag="PWU31_002165"
     CDS             complement(5149..5664)
                     /gene="lacB"
                     /locus_tag="PWU31_002165"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL1084662.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5683..6111)
                     /gene="lacA"
                     /locus_tag="PWU31_002166"
     CDS             complement(5683..6111)
                     /gene="lacA"
                     /locus_tag="PWU31_002166"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL1084663.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6131..6904)
                     /locus_tag="PWU31_002167"
     CDS             complement(6131..6904)
                     /locus_tag="PWU31_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1084664.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7069..7548
                     /locus_tag="PWU31_002168"
     CDS             7069..7548
                     /locus_tag="PWU31_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084665.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7605..7907
                     /locus_tag="PWU31_002169"
     CDS             7605..7907
                     /locus_tag="PWU31_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL1084666.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7929..9386
                     /locus_tag="PWU31_002170"
     CDS             7929..9386
                     /locus_tag="PWU31_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL1084667.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9979..10731)
                     /locus_tag="PWU31_002171"
     CDS             complement(9979..10731)
                     /locus_tag="PWU31_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1084668.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10946..11182)
                     /locus_tag="PWU31_002172"
                     /pseudo
     CDS             complement(10946..11182)
                     /locus_tag="PWU31_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11192..12150)
                     /locus_tag="PWU31_002173"
                     /pseudo
     CDS             complement(11192..12150)
                     /locus_tag="PWU31_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12356..14038)
                     /locus_tag="PWU31_002174"
     CDS             complement(12356..14038)
                     /locus_tag="PWU31_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL1084669.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
BASE COUNT         4140 a         3040 c         2388 g         4533 t
ORIGIN      
        1 aactcataaa aaatattttg actttcaaaa tcacttttgc taccagaagt tgaggcgaag
       61 tgttttaagc atagttgtac cctagtttga ccgttaaaaa taaataacga tcgctagagt
      121 gcaactatgt ttgtttgccc taccgtcagc gtcggcttgg cctctcacat ccattaccgt
      181 cgggattatc tccgttacat acccttacaa aatacgccct gtaagcattg catgaaacca
      241 ccgtgcattt gcaccgtatt tccgccctag ccgttcaaat cggtcgctac caccattcag
      301 aaacacgtgc ggtaatggaa ggtttggagg aagctatcca gaccactaac gattgtccct
      361 atcgtcgtac gtttcactca gatcaaggtt ggtcgtatca aatgaaagct tatagaaaca
      421 aattgaagga gtacaaaatc tttcaaagca tgtctcggaa agggaactgc ttagataatt
      481 cacttatgga gaattttttt ggcttattaa aacaagagat ttttcacggg aaagtttata
      541 actgtttcga ggaattaaaa tcagccatcg atagctatat ctattactac aataatgaac
      601 ggataaaaca gaaattaaac tggcaaagtc ccgtccaatt tcgtaaaaca accgttacgg
      661 ttgtgtaaaa atatcatttt agagcatgaa aaaaagagtg gatttctcca ctccaaaaaa
      721 gtctaacttt tgggggtcac tacatttttg atccttttta tttgattaat tatatccagc
      781 tggataataa ttttgggggc aacatttcta tcttactatt atcttcctaa agtagtagac
      841 tttcttattg ggaaaaatta caacaaaaag gagtggctta gttgacaact cctttttgtt
      901 tacaaatcaa ttggatatct ataaattgta ttttctaacg aattttatag acatctgatt
      961 tgtcaaatta agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt
     1021 gttcgataat ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata
     1081 aaggtttcat ccgtgtccct aaagtccatt gttttaggta agccatcctg cttgagccca
     1141 tgcactcggt actgatgaga aaaaattgat gcctgtttct cctgaggaat gccaattccc
     1201 tgatctttta cttcaataat cacgctaccc ttttggtttg aaatggtgac aattaccagt
     1261 tttccttcac catatttgat tgcattgtca actaaattcg aaaccgcccg ataaatccaa
     1321 cgatctcgtc catgaatcag ggttggttga tcttcagtaa acgcaaacaa aagattcgga
     1381 taattcattt tgtaggcatc acaaacttcc gcacagatca gtgaaacgtc tacgacttcc
     1441 gtgctaagat cctctgagtt attggataat gtcaaaatat cgtcaatact gtcggtcatc
     1501 cgatctaaaa gcattaaatt tccttgatcg gcatgttgcc gttctatttc cagattcgta
     1561 cgcaaaatag cgatggcatt tttctgctcg tgggtcagat aactatttaa tcgtcgataa
     1621 tcctccaaat gaccatcgaa cttatccttt agcttattaa aagccgcatt taatgcctcg
     1681 tccttcacag tatttttagg aacttgatca ataaacgtta aatcacgcgt aatcgctaag
     1741 atttgattac tgtaaatttt gcgtaaaacc aaccaaagaa taaacgttaa taacatgacg
     1801 gcagaacaaa taaccagcac catcgctggt aaatatttaa atagttcgtc ctggtaagct
     1861 ttatctatca tcgaaatagt cataaattga ttgttggaag atttcgaagc ttccatctgc
     1921 gtctctgctt gaaccgtctt accctcttcc ataaaataaa catcttcacc taaggtagat
     1981 gcgatactga ttccctccaa attatttctc agaaagaaga aactcactaa aatcacgcct
     2041 gtcaccaaca gtgaaaaagc cagcatcgtt aacgtcattt taaaatttaa tagcatagct
     2101 ggtacccctt tgctcgaatg gtttttaaga cctctttttc agctgcttca gccagtttct
     2161 tttttagacg agccagatgc actcgcagaa ccgacgaaaa aggatcaaaa tcttcatcgt
     2221 agacatgttc agaaatttcc tcagacgaaa caaccgctgg atgacgctga gcgatgcaca
     2281 gtaagatatc aaattccttg ggtttcagga ccacctctgt cttttgatag ctgacccttc
     2341 gacctaatgg atcaatcttc aaagcaccaa ttgaaatctg cggcagcgca cgtccatgat
     2401 aacgacgaat aaccgcgcgt attcgtgctt ttaattctag taactcaaag ggtttgacta
     2461 aataatcatc tgctccaaaa tccaagcctt ttgctcgttc ttcaatctca tcacgtgcag
     2521 taataatgat aactggactt tcgacttctt cagcacggag actttgtaaa acagataatc
     2581 catctttttt aggcagattt aaatctagta gtatcccatc gtatgaattg atgaaggcct
     2641 tctcttctcc tgactcgcca tcataagcta catctacaga aatattctcc tgctctaggc
     2701 ccttttttac ggataaaacc agctcacgat tgtcctcaac caccagtaca cgcatgtata
     2761 ttcactcctc atacggattt agtaattaca cttatgcttt ttagcatagt acacgctctt
     2821 cttcaaagtc cacgttcttt ttgtgaagat ttgaagaaat tcatacattc tgttaaaaaa
     2881 cacataatta agaatgattt tactgtttga aaatacctag aatagaaaaa gactggaacc
     2941 tttcctccag tctcactact ttctatttgc ttggttttct tgtatatact tttcaaagtt
     3001 cgttggttta tgaccaataa gcctcgttag aatactgaaa attgccatca agtcattaag
     3061 taccacatgt aagaaaatct ctctaaagaa aaaccccttc gttttaactc acttactatt
     3121 ttagaattcg ttcccctaat aatgcagcat gattttacgc tttatgtata aaaaaagact
     3181 gcaagcaatt ttgcttcaca gtctgatttc ttatgatagt gagcttgtta acacttccac
     3241 cggtgtagca gtttctttca atacggcatt taattcttca atatttttac gcccttgcgt
     3301 attcatccac tccatcgctg cttcttttcc atcaacgata tagctttcta caccatctgc
     3361 ccaagtcgca cggccgcaaa gtacaccatt gaaggttgag ccggcatttt tcgcgaagcg
     3421 cagtgtttct tggaacaact ctgcttttac accggcgctt aggaaaataa atggcaggtt
     3481 agtggcttca gcctgttcct tgaagaagtt agccgcttgt gcctttgtgt acacgacttc
     3541 gtctgtccca tagccttcca cataattcat attgacgggt acttccacct ttaacacatc
     3601 tacgccataa cgtggatcag aaaattcctt catcatatcg ttgactttat gaggtttctt
     3661 aatggcgtat tctttcgaag tcgcatcaga gatgttcgcg tcataagaaa caagctccaa
     3721 gaagaagggt aagttttcag cctgacattc tgagccgatt cgttcaataa aggcatgttt
     3781 ttgattattg atttcttctg cttcatcgac atcatagtaa agtaggaact tacaagcatc
     3841 ggcgcctgct tcttttaaac gtttaactga ccagatcgat aataaatctg gtaaacggcc
     3901 gggagttgac gcatcgtaac cagttttttc ataagctaac agcaagccgg cattttttgc
     3961 tctaacgtca gctgctggca agccatattc gggatctaat aaaatcgatg aggcatattt
     4021 cgttaattgt gaagaaacaa tttctttaaa ttcaacgatt tctgattctt ctgcttcgct
     4081 gcccttgaat tttgtgatca tcttcttcaa agccccgcgt tgatcaattg ctaaggcccc
     4141 aatcactcca tcttcattgc atagttgctt gatatattga cgtttttctt cgttcatttc
     4201 cgtatcctcc tacactttct ccactaagat ttgatcatat agtttttgat aattggctaa
     4261 ttggacatag ccagtcgtcg cttcctgagc attcagcatt cctaaaacat taccctgttt
     4321 taacacggct tcatccgaaa gaccttgttg aatcgcagcg gtcaatccgg caacggtcgc
     4381 atcaccagag ccaactggat tgacgacatc aatctttgga attgtcacgc aataaaaagt
     4441 ctcgtgatgt ttggcaaagg ctccgtcgcc ccccattgaa accactatcc attcaatttc
     4501 atcaaacatc gaatctgaca aggcggcttt taaatctgat aattctgttg agatttctcg
     4561 tcctagcaga ccagccagct cttcggtatt gggttttatt agcttaggct ttacactgcc
     4621 tcgcaacact tcccttaatg ctgtacctga acaatctaaa acgacaggct ttggctgaca
     4681 aacttgcagc atttgtcgat aaaaatcagc aggcaatcct tctggcaagc ttcctgaaaa
     4741 agaaatgatt tcggctttaa cagctaattt ttcgaaatgt tgcaagaacg cccttgcttc
     4801 ttgttcagta ataacaggtc cgttttctag gatttccgtc tgttttccgt catgtaaaac
     4861 agcgatgcaa ttacgtgtct ctccgctgat aggtaaaaag tcatgagcaa tctcattttg
     4921 agtcaggtcc tttttaatct cttcacctaa aaaaccgccg attaaaccac tagcaatcac
     4981 ctccgtatct aattgcttca agatacgtgt cacatttagg cccttaccgc cagctgtttt
     5041 gcgaacattt cttactcgat tgactgtgtc gagcttgaag gtttctaatg gataagaaat
     5101 atcaatcgat ggattcatcg ttattgttaa aatcatttgt ccacccgctt aatcgtggta
     5161 ttcgccacga tcccatttct cgataaattc atcgaagaaa tgcggatcag actgcttgtc
     5221 actggctttt tcgacgtgat cgattttggc gatcaatttt ttgttttctt cagtttcttg
     5281 ataatcggca ttgatgaagg cctctaatat gtcacagatc aaaaattctc ccgtaatccg
     5341 agcgccaaag ccgatcacat tggcatttag ttgttccttc gcatagatag cagtcgtcat
     5401 atcgcgaacc agcgcactgc ggataccagg tactttattg acagcgttgt taatcccaac
     5461 acctgttcca cacaaacaaa ctcctagatc tgcttctccg ctagtcacgg cctctccgac
     5521 tttttttccg tagatcggat aatgggttct ggtaaagtca taggtcccca tatccaacac
     5581 ttcatgtcct tttgatttta agaaatctga tactgccatt tttgtatctg tgacgatgtg
     5641 gtcacagcca attgcgattt tcatttcatt catccttcct aattacgcca ttttatttaa
     5701 catgtcgaca cgtacttgat gacgaccacc atcataattt gcttgtagaa attccttaac
     5761 aatattcaac gccaactcat cgccaacaat ttttgatcca agtgtgatca tccgagaatt
     5821 attatgttta cgggtcatat acgcactgcg ctcatccgaa acttctgctg ctaccattcc
     5881 tttaaccttt gttgccgtga taaagctgcc agcgccgtag ccatcaaccg cgattcctaa
     5941 actaccttcg ttttcattca gatcatttgc gatcgccaaa gttgattcca caaaatcttc
     6001 tgaagccgtt tcactcttat ctactactgt aataccttct ttgatcaatt ttttcttgat
     6061 taattccttt aattccatgc cgtcactgtc tgaaccaatt actacacgca tttttgtttc
     6121 ctcctatttt ttactgaatg attttagtga attgctgata ataactcttc agctcggctg
     6181 aaagagtaga atccgtaaat aatttgtcga tcttgcgcaa atcatagaaa ttataaaaat
     6241 cgctatggtc gaatttgttt gcatctgaaa ccaaacattt ttctgccgca ttatctaaaa
     6301 caattttttg caaggtacct tcttcaatac tgaaattcga aacctccgaa cccttcaatc
     6361 cattcactcc gacaaaagcc ttcttaatac cgattttttg aacagcttca ttggcaattg
     6421 aaccgacaaa ggctccagtc ttttcccgat atagcccacc gatcaaatac agttcaaatc
     6481 tttttatttc acttaataga ttgaaaaccg gcagactatt ggtaacgatt cggagagatg
     6541 acggcttgat gtatcgtgtc attaattcga tcgttgtacc cgatccaaga aaaatcgtgt
     6601 cgtcttcttg attgatatga gctgccgcat ttttagcgat gtattccttc tcttttgtat
     6661 gcagcttctt tttttcagta tgagaaagct catttgcttc tatagaagtc gacatttcca
     6721 gataatcgat tttttgtgca ccgccatgaa cgcgtaaaag ctttttcttc tgcgcgagtt
     6781 cctctagatc tcggcggatc gtcatatctg aaacattcag tacttcctga ataaatttga
     6841 ctgttatcgt cccatagcga tcacacatcg ctaagatcga acgctgtcgt tctgttttca
     6901 gcatattctg tacctccacg accttataat aaaacattta cgaacatttt tcaacacata
     6961 ttgttcgttt attttcgttt atgcgttgac aatgattttg taagcggtta tactcaactt
     7021 gtaaacatat aaataaacat aaacgaacta ataaaggagc tgttttgtgt gaatgaattc
     7081 tttttcaaag acactgtcta tgtttctgac aagcagtcgc aggaagaagt gttcgaggaa
     7141 gtctatcaag atctgttaaa gaaagaattg gtaaccgaag attttttgga gaacctttta
     7201 gaacgggagc gtaattatcc gacgggatta gatctctcac ctgtctctaa aatattgcca
     7261 gatatcgcta tccctcatac cgaaagtgag tttgtgcgaa ccacccgtat cattccaatc
     7321 aaagtaaaaa atgaaatccc tttctacaac atgatctcgc caagcgatca gatttccgct
     7381 tcatttcttt tcatgatctt gaacgaaaat ggtgaagcac aggctggcat gttggctaat
     7441 atcatggatt tcattaattc tgttgacgaa gaaaaactgt tttcgttttt caaattagag
     7501 gacacagaag cgatttatca ttttttagaa gaacattttt atatatagaa gtctctgcct
     7561 aaccgcagga taaaaaataa acttatttta aggggagaat tattatgatc aaaattttag
     7621 cagcatgtgg agcaggcgtt aattcaagcc atcaaatcaa aagcgctttg gaaactgaat
     7681 tgaaaaatcg tggctatcaa gtcacctgtg acgccgttat gatcaaagat atcaatgaag
     7741 aaatgctgtc tcattatgac attttcgcac aaattgccaa caccgacttg ggttttgaca
     7801 ttaacatccc tgtcgttgat gcaggtgcta ttctttatcg tattcctgct atggctgagc
     7861 ctgtttacca aagtatggaa agcgcaatcc aatcattaaa caaataggac taaagaaagg
     7921 aaaagcatat gaatacaatt attgatattg ccaataaagt atttaccccc ctaattaatt
     7981 taggtgcggc accgttaatg accatcgtat taactgttat tgctttattt tttaaagtaa
     8041 aaccgtcacg cgctcttgaa ggcgggctca aattagggat cgccctgact gggatcggag
     8101 ctattattgg aattttaact actgcttttt cagacgctat gacagctttt gttgaacgca
     8161 caggtatttc attaaacatt acagatgtcg gttgggcccc actagcaacc atcacatggg
     8221 ggtcgccata cacgctttat tttttactaa ttatggtgat cgtcaatatt gtcatgttag
     8281 caatgaaaaa aaccaacaca ttagatgtgg atattttcga tatttggcat ttagcaattg
     8341 ttggtttgtt cgcgatcttc tgcggtgcta atctgtttat tgcttctgca ttagttattt
     8401 tcattggtgt attaaaaatt tggaactccg acttaatgaa accaaccttc aatgacttgt
     8461 taaatgcgcc agacagcaac ccgatgacca cgactcatat gaactacatg atgaatccga
     8521 ttattatggt tttcgataaa attttcgaca aaatcttccc ttggttggat aaatatgatt
     8581 tcgatgctgc caaattgaat agtaaaattg gcttctgggg atcaaaattc gcgattggaa
     8641 tttatttggg gatttttgtt ggattactag caggacaaac accaacgcaa attttctcgc
     8701 tagcctttac cgctgccgtc tgtttggaat tgttctcttt gataggcgct tggttcatcg
     8761 cagccgtaga accgttatca caagggatta ccgattttgc caataaacga ctaaaaggac
     8821 gtaccatcaa cataggttta gactggcctt tcatagcagg ccgcgctgaa atctgggctg
     8881 ccgctaacgt attagcacca atcatgttgc tagaagccat catcttacca ggaaataaat
     8941 tattgccact aggcggaatc atcgcaatgg gtgttacccc agcactattg gtcgtaactc
     9001 gtggaaaatt aatccgtatg atcgtaatcg gcgcgatcga attaccatta ttcttatggt
     9061 ccggcacctt gatcgccccg ttcgttactc aaacagctaa agcagtcgga gcctttccag
     9121 ccggcttgaa cgggtccgcc atgatctctc atacaacgat ggaaggcccg attgaaaaat
     9181 tcctaggtta tttagtcggt aatgcttctc aaggacaaat cgaatttgtg atatacgctg
     9241 ccttagcatt aggtgcatac cttttaatct tcatttggta cgctcgtcaa atgaaaaaac
     9301 gcaacgctgc atacgcagct gaaaaagaag agaatgcatc tccggcaacg gcgcataata
     9361 tgacttatgc agccgaagct aaataagcta aaaacacccc gaatgtcttt agacttcggg
     9421 gtattatttt acctataaaa aataaataga tgtaaaatcc atttcaaaaa gcatagccca
     9481 tctcttgcca cacccaataa agaaattatc aacgtttatg ctttttttaa ctcctttaag
     9541 cttgcctttg ttggtattcc tcaacgattc tttttgtgtt cttgtttgtt ttgtgctttt
     9601 tgactttttg ttgtttaatt gcgaattcct tgatttcttt tttcctgaat cgcttcgcat
     9661 cccgcggtta tgcgtgcgat ttcatcattg ctttttctga tctaactcgt ttcatgtcaa
     9721 agagcttcag ctgacttagt gtccatggtt gttttgatat cccttttcct ccttaataaa
     9781 gcggtgattg atactgctat tttttccttc acataaacat aacatcccaa aaaaatcgac
     9841 tatctttttt caaatggtgt attcaagtat tcggaggaat tagtaatgat gcaccctaaa
     9901 aataacataa ttcgcttaaa ttcaaggcta ccaaaaaact accaagttct aattatctag
     9961 agtttggcag tttctcattt acacaacata tttttcttct ttttcgattt tttcatagtg
    10021 cttttgttca tcatcatccg ttataactac tgttacatca ttcagattat aatatactga
    10081 aaaatctctt ttcccaattt tcgaatgatc aatcaataaa aagcgttcaa cagaattatt
    10141 cagagcgatc tgttgagttt ggccctcttc aaaagtcgaa gtcataattt tctgttcatt
    10201 taaagcatta cagctgaaaa atgctttatg aaaattcata tcctgtagcg atttattcgt
    10261 gatttcacca acaaagcatt gagtcgtttc ccgcatctcg ccacctaata gatagatttt
    10321 tcgattccct tttcgcttat ttaattcttc aaaaacaggc aaacagtttg taacaatccg
    10381 cagtgaatcc ccttgaatga tttttgacat gagctcgatt gtagttcctg gtccaagaaa
    10441 tatcgtatca ccatcattga tcatttcatt cgccttttcc acaatccgct gcttttcttc
    10501 ttgcagcata atctgttttt ctctatgaga aagctccaca tgccgataaa aatccttcaa
    10561 tttcgctccg ccatggacgc gttctaataa tccttgtttc tccatttcgt ccaaatcacg
    10621 tcggactgtc atatccgaaa ccgaaaactt ctccataata tcgctgactc gtactgtacc
    10681 gttaacctct aataacgaca tgatcgcttg gaatcgatcc gtttttaaca tttcatcgcc
    10741 tcttttcttt ttaagactat caaattctta agaaaagtgc tagaacaagt tttcttaaga
    10801 agccttctgc aggaaggaaa gcaaaatttt tcagaatttg caagctgcta ttctaaaaaa
    10861 taaaaccgaa cgagcatgac tgtcatagtg acagccttgt tcattcgact tttatcttta
    10921 ataaatcatt ttatttaatc tgaagttatt gtgcaagttc aaaaacaaag tagttaatgt
    10981 ctcctcgata tttcgaaatc gaatactcaa cgggcagacg ttctagcgaa aaaccctgtg
    11041 tatggaaata gaacattgga tctccttctt ccacatctaa caaatcgctg atcgtatcat
    11101 ccgctttgat tacttctagt tttctggtga ccgttttaat tggataaccc atttcttcaa
    11161 aaacttgata cattttttct tttctgattt gtcaaattaa gctagacaat atgtctttat
    11221 ctatgcagct caaaaatact tccaatggtg ttcgataatt taatgatttt cttggaatat
    11281 ggtttctttt cgaggcaacg gattgaataa aggtttcatc catgtcccta aagtccattg
    11341 ttttaggtaa gccatctcgt ctaagcaagc cgtttgagtg ctcatttagc cctctttgcg
    11401 aaggtgttcc tggatctgcg aaaaagatgg caatatcgtt cgagttactt atagatcgcc
    11461 agttggaaaa ttcttttcca caatcaaatg tcatggattt aaacaagtgt ttaggcatgg
    11521 atttgaacca atcatttaac ctgttttcga tatcttcggc tcgtctgcct gtcggtttca
    11581 aagtaataat aaccttggat aaacactcaa ccaatgtaat aacagcgctc ttgtgtttaa
    11641 ctcccacaat ggtgtcgcct tcgagatgac caaattcttt agtcaggtgt ggatagagac
    11701 tgttccgttc atgaatcgtt ctgcgaaagg cctgttttcc acgcttttct tgatgattat
    11761 tcggtttacg tttccccttc ataggtaacg actgtgtgtc aaaaaggcct tttttaaaca
    11821 tccgatagag tgtacgaaca gaacaatcaa ttggaaactc gccacggccg ataatcacat
    11881 cgggtgacca cccttgagct accttttctt gaacatatgt tgattgttca tcagataaaa
    11941 cggttgaacg tcgtccacaa cgagatttat tttgtttgta ttgacggtaa taatctagca
    12001 cttttttcct tgtttgagaa acgaataaac attataaatg gtttgtctag agcgatttaa
    12061 actttgtgcg acaagagcga caggtttctc gatattgaaa taagactcta tgattactag
    12121 ttcgtctgtt gtaagatggg tgtaggtcat ttgtactcac tccttattaa ttctttggtc
    12181 ggaactattt tgagtgtagc acaaatgatc tttttagttg tctagcttaa ttttacaatc
    12241 gacgcctcaa aaagaatacc cgaaatttaa acagcaaaaa aagatagcgc ttaccattgt
    12301 gtttaagata aaacgctagc taagttggct agcgctttat taatctaagc aagctctaaa
    12361 attgcttcaa tacaaaatca actgctcctt taggatcacg tgttaaattg atatattctg
    12421 cacctttagt tgcaattact tttatcccta atttgtctgc atctttacgg agatcatcca
    12481 aataggaatt cacttgaggt gctagaacga ttaaatcaaa atttttcatc atatcataat
    12541 gagaaccata agctcctgct ttagcagaaa gattaacacc tgtctctttt gcgccctctt
    12601 ctaatgcatt agccaacatt gcactcgttc cggcaccagc acaaagtact aaaacgcgct
    12661 ttccttcacc aaccgatgct tttgcttcat caactataac tgccgcagtt gcaccaactt
    12721 cttggatttc ttcgaccaat tcttctttta cttcttctgc agcaatgttt tttaactctt
    12781 gttctactaa ttcagaatca taggccttaa caaaaggtaa ataaataatc gcatcgacaa
    12841 caatcaatac taccgctaat acaatcgcca acacactaaa cccggttcct aaaaccaagc
    12901 caatcggtgc gggcgtcgcc caaggcaata cggatataaa gctattcatc cccaagacat
    12961 cgacaaaaag tttgaatatc catacgttag cgattggtgc taataaaaat ggaattagaa
    13021 agtacggatt caaaataata ggagtagcaa atagtaacgg ttcatttacc gcaaacgaaa
    13081 ctggaataaa tgaagcttta ccaactgctt tcaattgttt tgatttagcg aacaacatga
    13141 acagaaaagg aacaactagt gtcgctccag taccacccat cgttccaaca aagtttccta
    13201 gaccaactgt taatactttt gaggcttgtg caccgttttt aaataattca aagttagttt
    13261 ctacgttcgc ataaataatt gcagcaatcg ctggttcaac aatcgatggt ccatgaacac
    13321 caacaaacca gaacattgcc atggctcccc aaatgattgc aataccgaca tatccatcag
    13381 cagcactaaa taatggctgc aataattgaa ttacacctgc tgcaaatgat gttccaaaaa
    13441 attgacgaac tcccgtatca atcaaaacgg caataaatac tgcaattgaa aaggggataa
    13501 tatctctaaa catctgtgtg attgttccgg gaacttcttt tggcatacgg atcgtcaaat
    13561 ctcttttaac acagaattta tacacattta cagtaacaaa cgcagaaacg aaagctgcta
    13621 ataatccctt tgtccctaaa taatcagtag ctatcccacc atcaatttga tcagctgcca
    13681 tcattacaaa accacaaatt gacgctaaca taacagaaac ttcattaatc tgcttacctg
    13741 aatcagataa ttgacggttc attgaattag ctaaacatcg agctgttgta gcaacaacca
    13801 aaagaccaac taatcccatt gaataaccat aaactttcca aagccacgtc gagaatgcct
    13861 ctggtaacgt cacgttaaat acttctggaa cagccgcaat cataataaaa atacttgaaa
    13921 atagaatagc tggcatagca gttaaaaagc catcgcgaat tgcccctaaa taaatattac
    13981 gtgagatttt ttcaaaaaac ggttttccct tctcaatttg cttaataatc gtattcattt
    14041 ttcttctcct atgctttagc aaatttttct ttgtacaatt caacaaaatc attgataagt
    14101 t
//
LOCUS       DANJMA010000057        13881 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000057 DANJMA010000000
VERSION     DANJMA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(541..975)
                     /locus_tag="PWU31_002175"
     CDS             complement(541..975)
                     /locus_tag="PWU31_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084670.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(992..2197)
                     /locus_tag="PWU31_002176"
     CDS             complement(992..2197)
                     /locus_tag="PWU31_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL1084671.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2891..3277
                     /locus_tag="PWU31_002177"
     CDS             2891..3277
                     /locus_tag="PWU31_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1084672.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            3371..3562
                     /locus_tag="PWU31_002178"
     CDS             3371..3562
                     /locus_tag="PWU31_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084673.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            4076..4408
                     /locus_tag="PWU31_002179"
     CDS             4076..4408
                     /locus_tag="PWU31_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1084674.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            4704..4859
                     /locus_tag="PWU31_002180"
     CDS             4704..4859
                     /locus_tag="PWU31_002180"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL1084675.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            5135..6475
                     /locus_tag="PWU31_002181"
                     /pseudo
     CDS             5135..6475
                     /locus_tag="PWU31_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6716..8332)
                     /locus_tag="PWU31_002182"
     CDS             complement(6716..8332)
                     /locus_tag="PWU31_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL1084676.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            8450..8668
                     /locus_tag="PWU31_002183"
     CDS             8450..8668
                     /locus_tag="PWU31_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084677.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8863..9324
                     /locus_tag="PWU31_002184"
     CDS             8863..9324
                     /locus_tag="PWU31_002184"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1084678.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(9517..9756)
                     /locus_tag="PWU31_002185"
     CDS             complement(9517..9756)
                     /locus_tag="PWU31_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084679.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9780..11303)
                     /locus_tag="PWU31_002186"
     CDS             complement(9780..11303)
                     /locus_tag="PWU31_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL1084680.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(11321..11443)
                     /locus_tag="PWU31_002187"
     CDS             complement(11321..11443)
                     /locus_tag="PWU31_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL1084681.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(11473..12456)
                     /locus_tag="PWU31_002188"
     CDS             complement(11473..12456)
                     /locus_tag="PWU31_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL1084682.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(12480..12629)
                     /gene="rpmG"
                     /locus_tag="PWU31_002189"
     CDS             complement(12480..12629)
                     /gene="rpmG"
                     /locus_tag="PWU31_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1084683.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(12650..13027)
                     /locus_tag="PWU31_002190"
     CDS             complement(12650..13027)
                     /locus_tag="PWU31_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084684.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(13059..13238)
                     /gene="rpmF"
                     /locus_tag="PWU31_002191"
     CDS             complement(13059..13238)
                     /gene="rpmF"
                     /locus_tag="PWU31_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1084685.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(13253..13522)
                     /gene="rpsN"
                     /locus_tag="PWU31_002192"
     CDS             complement(13253..13522)
                     /gene="rpsN"
                     /locus_tag="PWU31_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1084686.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         4494 a         2598 c         2072 g         4717 t
ORIGIN      
        1 taggaatctt actaacctta tgttaactaa tttacttaga gaatttcatg aacattacaa
       61 caaaaaaagt acatttcttc atgttttttt cggatatttg tgctttttaa ccattcagca
      121 gctgaaaaca tataaaaaga tatgtatgta ttctttctga aattaagttc atttctatgc
      181 gatgaagcat gttttcaaaa tattaccctt tcttagatta aacttcagag tattcaaata
      241 acggaataag gagccttatc ccgcattact gaatgagact gtgacataag taaaaaggtg
      301 acttcaaatt ccttcttcgg aatttgaaat caccttcttt ataattataa agtatcggtt
      361 tcacagcatt tctgaatgat tcctagaatc ttaaacgaat gattggagtt atcaacattt
      421 tttcactcgt ttttagcgaa acaaaaaatg acatttgtca cagcctcatt aaattattga
      481 ttctttttct ttataattga attgtccata tttaaagtgt ctagtactct acatgaataa
      541 ttaattgctt tcactcaaat acttataatc tattaacttg tagttgttta acttttctga
      601 aaaagttaga cccacgtaat aatcttccca gctttctctt tcaattaact gatccaattt
      661 ttgataatcc ataaattcat catgtatcaa aaagaataaa taacttgata ttttagcatc
      721 tagcttaatc agattaaata atgtttgttt aaaattgcta ccattaatat gactgataga
      781 ttctctagct tcttcacata aactttttac tttattaatt actactttgt catattcatt
      841 taacacacta ttattaccat tacttagacg ctcagattct acgaaacaac tcaaatcaga
      901 aatttcccaa aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa
      961 atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa ttgtccacta
     1021 aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt
     1081 gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca
     1141 acattatttt agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca
     1201 tttcattctt aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt
     1261 cctttacagt aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta
     1321 agggagaaag atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag
     1381 aatccctcac ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag
     1441 catctctact ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta
     1501 acttttcaaa aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac
     1561 ttggaattat tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat
     1621 ttttaacata ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat
     1681 tttgtgataa cagattatct attttgattt gctcgatagt tttacttaga ttttctgtat
     1741 attcaattaa cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta
     1801 aaatcagaat atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat
     1861 agagtcgttc tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaaa
     1921 ctttatcaat attatttgct ctaaatggac caaataagta atatctggtt ttaaacatgt
     1981 atagaagaaa catctcatta aaatcaccag taaagaaatg gaaatcttta gtttttttaa
     2041 tattatcagt tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat
     2101 actcttttat aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat
     2161 gaagagactt taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct
     2221 acatcattaa atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct
     2281 aacttacttc attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt
     2341 acattttatc accgagaact catcattttt catcatgatg aatactattt acatatatct
     2401 agttcaaaga caataattat tatgatccgt tattatctaa gcactataca gataatagga
     2461 atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt
     2521 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
     2581 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
     2641 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
     2701 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
     2761 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
     2821 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
     2881 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
     2941 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
     3001 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
     3061 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
     3121 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
     3181 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
     3241 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
     3301 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
     3361 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
     3421 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
     3481 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
     3541 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
     3601 tttcaaattt cgaacaataa atttgaaact atcttcttta taattataaa gtatcgattt
     3661 tacagcgttc tataatgatt catagaatct aaaacgaatg attttaatta tcaacgtttt
     3721 ttaattcgtt tttagcttaa aaataacttt tgtcacagtc tcactcctaa aattacttta
     3781 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
     3841 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
     3901 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
     3961 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
     4021 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
     4081 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
     4141 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
     4201 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
     4261 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
     4321 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
     4381 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
     4441 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
     4501 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
     4561 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
     4621 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
     4681 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
     4741 atcggtcttt tatttatcaa gaactgttga acgaccacca catcacacat tcagtgagcc
     4801 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     4861 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     4921 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     4981 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     5041 ccaatacttt ttcttttttc taataaacgc aaacagacac cttaaaatcc tttagaataa
     5101 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
     5161 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
     5221 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cggaggaatg
     5281 ctttcatcgc cactatcaaa atgaacaaac gatcattaaa tagggctgga aaaaagtatc
     5341 catcttattg aatgatgtca gtaactacaa gaccattctg cggattaaca aacagcgctt
     5401 caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagtg atcgtcgctg
     5461 ctccattgct cgcagagtca aacaagccat tcttgagcta ttaagcgaac ctctttctat
     5521 gtctttgatc gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag
     5581 ccttcggcct aaaaccattt ctttgagcca gctgttacca gaagtggtgt gtttcgatga
     5641 attcaaatca gtgaaaaatg tctcgggagc catgagcttt gtcatgatgg acggcaagac
     5701 ccatcaactg attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt
     5761 gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg gtttcagatt tctatacacc
     5821 ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca
     5881 tattagccaa cacattggtc gggcttttac caaccaccga attcaagtaa tgaagacctt
     5941 taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac tggaaactac ttcaaaagaa
     6001 tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac
     6061 agaagcagag atagtcgatc ggctattatc ttatgatgat tccttaaagc gaggctacga
     6121 agtctaccag gattttcttt ccgttatcag aagacaagat gttcctgaat tcgtcgcgct
     6181 cctaaaagaa gactacaagg agttgccaga gcactaccaa ccagtgttca cgaccttcaa
     6241 gaaataccaa acagaaatca aacgagcgtt gcgcgttccc tattccaacg gccctataga
     6301 atgtttgaat aaccatatca aagtactaaa acgaattgcc tatggctata gaaactttca
     6361 aaactaccga gaacgaatct ttttatatcg aggaaaatat tttaagaaaa ctaagaatac
     6421 tacccaactg actaaagcca gaacaacaac acggctagac aagatagctg tctagccgtg
     6481 tgagtatctc gattattcta ttgaatttct tcaccaacac tcattgacga agagccaaaa
     6541 taagtaatct aaccgactgt taactaaacc attgcaggtc ccgttaaaga tacttgtgcc
     6601 tgttgtcacc gcttcttttt cttgtccaca agcgcttaac acacttaata aagcccctgc
     6661 tattattaat aataatatct ttttcttatt aaaaacctcc tcatctattt ccagattagt
     6721 aaatgtagta atctgtgctt tcttttttct caatactaat aactaactta tggggtaaac
     6781 aaatactcgt ttgaccgggt tttgagatcc atccagttct aaccgcgatt tgatcaggac
     6841 tattatcttc tttaactcga attcttccat ctttaatctc aacaatatta tattgtccct
     6901 tcgcaggatg ataattcacg agtttatgat tgatcttatc caaatcgaag cgatccattt
     6961 ccttgccatc aattcgaacg atggcatatt tgatagcatc ttcagttgtt tcattctcct
     7021 tttctataga gaatacaaat aagggaataa aagaggtaat aatcaaaatg ccaataatta
     7081 tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt cattaacgat atctggctaa
     7141 acatattaga ctttttccat ggaaatggtg aaatttttcg gaaattatct gcattaagag
     7201 aatcagttaa acaaccctca agttgttcct taatacattg ttgatccatc ttcataccaa
     7261 taaaaactag tttattaatg cgttcaccaa acaacggatg ccaggcttcc ttcaattcgg
     7321 gatttttttg tataatcatt tgacactcaa ttttaggtaa actagctacc caataattta
     7381 atgattcaat acttttatag ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt
     7441 gggcaaacca aagaaaccct tttgctcttg taatattgcc aggaaaattt ttatccgtaa
     7501 gatgggcaaa tttttgtgcg tcaaggagta accgatttcg atagataaaa cttgagatac
     7561 catattcttc tgtttcagga atatgatttt gatagccaag ctctaactct ttgaaccagc
     7621 ccactgaata ggaagtctca tctatattaa aaagatttgt atttagaatt ttcgataagt
     7681 ctacttcacc tttaattgtc ctaattattt tggcattgga ctgtaactta gcaattagac
     7741 cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact attatttaaa agtaatatgt
     7801 tacaacactc gaattgatca attaacaagc cacgaatatc ctgttcttct tcaactacat
     7861 taccttcagc ttttctttcc tgaatctttt cacccgtttc gaaattatcc caaaaacgtt
     7921 ttacatccac tactataata atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta
     7981 aagggttttt agctaatata attgcttgaa taattggaat tggtttgctg attcctgaag
     8041 cttcaataac aatttgattc aagtcagaaa attgcactaa ttgatctagt tcaacaatca
     8101 aatcttcgcg taatgtacaa caaatgcatc cattcggcat cgaaacaagt ttttctccag
     8161 ttcttttaaa acctttcttg tcgactaata ccccatcaac atttacttcc cctatgtcat
     8221 ttacaatgac agcaatttcc tttccgtgat caccgtacag aatatgattt agaagtatcg
     8281 tttttcctga accaagatag ccagttaaga ttgtaatagg tatttttttc atagggaacc
     8341 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     8401 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     8461 tcgtcatctt actatactcg aacgagaaag aatctttctg cttcacgaag aaggattttc
     8521 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     8581 aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     8641 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     8701 ttatttgtcg attttcagtg gtcgccagag gaaatctcga atcgtttgaa gcatgaacat
     8761 atggactttt caattagttt tatcacgatc taacgtgcaa tttgcaatga tcttttcgat
     8821 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     8881 acaagacata caaaaaatca tgtcgagtct cgtggaaaga tttgccttac tcattcgatc
     8941 catggccgtc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     9001 gtagctggca aaactggtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     9061 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     9121 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     9181 gaattctcca atcatttcgc agttacagaa gcattgaata atatgccctt ttattttcca
     9241 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     9301 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     9361 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     9421 ttgataaagt gttgcgcttg atttgacaat tcaagacata ataaaaagca gatcaaatcc
     9481 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     9541 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     9601 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     9661 tgtactccca tttaatctca tttgctttgc aaatcgataa cgccgataag catcaatatt
     9721 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa aattgaattt
     9781 taatgagcca tcatttcttg agcaatttca tgtttagaca atgaagctgg atagaaagta
     9841 ggccaattat gcaattcatt atagagtttt tcttgacttt ctccaccaaa gaaaatatgg
     9901 aaatgctctg ccttacttgg tgcaattcca tgatcactaa attgaatata tttaaaacga
     9961 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
    10021 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
    10081 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
    10141 taataatctt tgtactcggc agctgtcatg tctttattga tttttgcttt gtaatcgaaa
    10201 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
    10261 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
    10321 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttttct
    10381 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
    10441 tttgtaagac tttcaagagg attcagtact tccaatttta tcccagcttc gttggccaaa
    10501 gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
    10561 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
    10621 ccagcaatag gaacttgttt caatccataa tccaaagcta aatatccaaa ggctgcgtgt
    10681 tgcgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
    10741 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
    10801 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
    10861 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
    10921 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
    10981 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
    11041 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
    11101 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
    11161 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
    11221 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
    11281 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
    11341 tatttcgttt atgttctttg ataattttta atgccctttt tctagtttta atattcgggc
    11401 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccccaat
    11461 cactacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
    11521 ttcatccaac atttttccga ttagaataat agtggtttgg tctttttgtc gagtggtgta
    11581 ttgaaaatta acctgttcgt ttacctcttg catggcaact ataaaatcct gctcttgtaa
    11641 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccagtcaat
    11701 ccattggagt aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
    11761 ctcatgtact tgatggtgtt gatgatagta tttgccatta tagtgatttt ctgatgaatc
    11821 actttcaaaa actaagggat gattaaattt tttcaaatta taaaaatcct ttttttgaat
    11881 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
    11941 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
    12001 tgccatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
    12061 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
    12121 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
    12181 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
    12241 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
    12301 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
    12361 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
    12421 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
    12481 tatttcactt ctttaaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
    12541 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
    12601 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
    12661 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
    12721 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcgata cattgaaata
    12781 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
    12841 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
    12901 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
    12961 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
    13021 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
    13081 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
    13141 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
    13201 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
    13261 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
    13321 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
    13381 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
    13441 ttaattcctg acgtttatct gcatactttt caatcatcgc catttgtttc tttgctttag
    13501 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacacctc
    13561 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
    13621 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
    13681 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaggctctg aaaaataaga
    13741 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
    13801 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
    13861 atcgccaatt ttttaaaact t
//
LOCUS       DANJMA010000058        13826 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000058 DANJMA010000000
VERSION     DANJMA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            825..1298
                     /locus_tag="PWU31_002193"
     CDS             825..1298
                     /locus_tag="PWU31_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL1084687.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1307..1534
                     /locus_tag="PWU31_002194"
     CDS             1307..1534
                     /locus_tag="PWU31_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084688.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2170..2541
                     /locus_tag="PWU31_002195"
     CDS             2170..2541
                     /locus_tag="PWU31_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084689.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2797..3039)
                     /locus_tag="PWU31_002196"
     CDS             complement(2797..3039)
                     /locus_tag="PWU31_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1084690.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3071..3973)
                     /locus_tag="PWU31_002197"
     CDS             complement(3071..3973)
                     /locus_tag="PWU31_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1084691.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3986..4174)
                     /locus_tag="PWU31_002198"
     CDS             complement(3986..4174)
                     /locus_tag="PWU31_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL1084692.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4188..4751)
                     /gene="amaP"
                     /locus_tag="PWU31_002199"
     CDS             complement(4188..4751)
                     /gene="amaP"
                     /locus_tag="PWU31_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL1084693.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4789..5682)
                     /locus_tag="PWU31_002200"
     CDS             complement(4789..5682)
                     /locus_tag="PWU31_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1084694.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5760..6683)
                     /locus_tag="PWU31_002201"
     CDS             complement(5760..6683)
                     /locus_tag="PWU31_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1084695.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6732..7082)
                     /locus_tag="PWU31_002202"
     CDS             complement(6732..7082)
                     /locus_tag="PWU31_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1084696.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7115..8017)
                     /locus_tag="PWU31_002203"
     CDS             complement(7115..8017)
                     /locus_tag="PWU31_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1084697.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8010..8867)
                     /locus_tag="PWU31_002204"
     CDS             complement(8010..8867)
                     /locus_tag="PWU31_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1084698.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9201..10010
                     /locus_tag="PWU31_002205"
     CDS             9201..10010
                     /locus_tag="PWU31_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1084699.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10050..10547
                     /locus_tag="PWU31_002206"
     CDS             10050..10547
                     /locus_tag="PWU31_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1084700.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10932..11005)
                     /locus_tag="PWU31_002207"
     tRNA            complement(10932..11005)
                     /locus_tag="PWU31_002207"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10969..10971),aa:Ile,seq:cat)
     gene            11192..11515
                     /locus_tag="PWU31_002208"
     CDS             11192..11515
                     /locus_tag="PWU31_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084701.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11679..11933
                     /locus_tag="PWU31_002209"
     CDS             11679..11933
                     /locus_tag="PWU31_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084702.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12003..12248
                     /locus_tag="PWU31_002210"
     CDS             12003..12248
                     /locus_tag="PWU31_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1084703.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12324..12689
                     /locus_tag="PWU31_002211"
     CDS             12324..12689
                     /locus_tag="PWU31_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084704.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12750..13313)
                     /locus_tag="PWU31_002212"
     CDS             complement(12750..13313)
                     /locus_tag="PWU31_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084705.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4048 a         2562 c         2296 g         4920 t
ORIGIN      
        1 aggtatgtcc tatgatgaaa agattgtact attcattgat cataacgatt ggctatttga
       61 ttgtatcaaa tctaggaaat atggtatttg gtatcagtaa agaattcagt tggactacta
      121 cattatggga atctctattc tttttcatct tcgtattttt gttacaaaat tatcgaaaaa
      181 aataaacatg tgccatctat ctgttggcac atgttttagg atttatggta ctttttattc
      241 ggcaatccat tttatttttc tacttcttct taatacctct aacacttttg atttataaac
      301 taatccaatg catctacttg cgcttattaa gctatttcag ctagatagta ccaccccatg
      361 cgtcaatgct tgattttcct tttgtataac attgtataag caataaagga caaataagtg
      421 gtgattacac caaaccattg aataagaaaa attctgatac gtaaataaac atgaacttga
      481 aaaaattaat gaatcggtta gctactacgt agttgtcttt tttctcatac agttctgtta
      541 acttacactt ctcttcgcta gataataaaa aatcctccaa ctgtattgtc caatataatc
      601 ccaggcatct aaactagcat tatagaagag acacacatag aatctaaaat aactggtttt
      661 aaatgtttct tacaatcaaa aaaagctgtt aaatgcactc cacaccttca tactctatca
      721 ttttttataa acgatatatt tttattgttt ttcgataaaa aatagttgtt ttacaataat
      781 tattctgcta tattttagct atcaaagata aaagagggat aaaaatgaaa tttaaaacat
      841 tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt tattttgttc ggcgtacttt
      901 tttatactca gatcgctaca agcgaaaaag gattcaattt tgattggcag accacattat
      961 ttgtttctgt catttacttt gcccttctcc tagggatcgt tattgcttat ttcttattga
     1021 aactattgaa aaaaatcgaa gataaccaag ctttttccac tcaaagctta aacatactaa
     1081 aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt ttttggctta cttcctaaat
     1141 ttatgaaagt agcggattta gacgatgctc caggtttgat gttggtagga atcgctgtcg
     1201 tattcattcc ttttgcagta tacacattaa caagcgtgct ggaaaaactg cttgaacatg
     1261 cgattttact taagacagat catgatttga cagtgtgagg tgcgacatgg caattatcgt
     1321 taatttagat gtagtattag ctcaaaaaaa aatgagtgtg actgaattga gtgagaaagt
     1381 gggcattacg atggcaaaca tctctattct aaaaaatgga aaagcaaaag ccatccgttt
     1441 ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt tgttgaccta
     1501 taaaaatgac tcgaatacta taccagaaga ataaaataaa gtagaacaaa cagtaaataa
     1561 aaaataatct gaatgataga tgtgtgagac ttttagaagt tatttacgtc tattattcag
     1621 attttttatt tcgattgaat accgttttat ctataaataa gatatgtact gactcataca
     1681 aagggggcct tttcttctcc atgtactatc acttttcctg atacaactgg tttcctgtta
     1741 cgttgtagtt ggatctgctt ttagtataat actttattca ttccaccaga ctgatagcag
     1801 tttgagcagt ctaaataatc atgtagtatt acaggccatt ctgtttcata ccttgaagtg
     1861 cttaaaactt cagctaatgg cccattgtgt taactcttac tccattcttt tttagtaagc
     1921 tcccatatat actgtaaagc cttctatata ttatgtcatg ttgcttttta acatttacta
     1981 tgtagttttg ataaaatgat ttgaataaca gtgatggaca attctttttt atttttaaca
     2041 ttattttgat aataatgatt atttatttaa aaatacattg gataacattt gttacgacac
     2101 gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca cacaattagg
     2161 aggaatagta tgccatttat tgtttacttt tttctttcat tactcgtgat ctatattcca
     2221 ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc aacatgatac gattgctgct
     2281 cttacgaaac tttgtttact tctatttgtt tgtctagatt actttacgag ctattattgg
     2341 ctaaaacaga caagagtaaa aaagagatat tatttcttcc ttgtcttttt caatctcttt
     2401 gagtttctga tccattgctt tttacttcat cagtatcata cgatccattt gtggatcatc
     2461 tgggttttca aagtggtcgt attattttgt atgttcacct ttcctatctc aagaaaattt
     2521 agggaggcat tgttttatta aagaagcttc tctgtatcaa taaagacaca tttaaggaaa
     2581 aacttttaaa aatctaattg ggaatttgcc cgcataaata agaggctgtg acaaaagtct
     2641 tgtcacagcc tcttattccg tataaacagt gttcaaaagc ttgcatcttc gataatatgc
     2701 ccaaccaaat gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgaa
     2761 gaacagcttt ttcacgacct ctttttaaaa gtctttttac agttttcgag caaagaatga
     2821 tacgattagt actaaaatga tggcaccaat caatgatggc acgattgcca ttcctgcaat
     2881 acttggtccc caagaaccta ataatgcttg accaagtgct gatccaacta aacctgctac
     2941 gacatttgca ataatcccca tcgaagaacc tttattcgtg attgctcctg caattgctcc
     3001 gataattcct ccgacgataa gtgaccaaat aaatcccatg tttcgttcct cctaatgttt
     3061 tacttcctta ttactgaacc cgtggttctg tattttcttt tactttttgt gcgccgcttc
     3121 cagctgctga ttttacttta tctgtttgat tcgaagcaaa ttcaccggtt tttttcgctg
     3181 cgtcagttac tttgtcttgc accgtttctt gatctttttc aaattcttct tttgttttga
     3241 tgtccactac gttagcgttg acttcgatca cttctaaatg tgtcatattt tttacttctt
     3301 tgccgattac ttcttgcatt tcttcaaaga ttttttcgat atctttgcca tattccacaa
     3361 tgacatccat atcaactgct acttgttttt taccgacttc tgtttcgatc ccagccgtta
     3421 cattatctgt attcacaagt tttcctgcca cattagagaa gaaaccaccg tttgcagata
     3481 ataaaccgtc tacttgttca attgcgattc cgatgatttt ttgcacaact ttatcttcat
     3541 aattcagctc accgcgcatt gaatcttcct gttcagaatc atgctttcct tggttatttg
     3601 tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc agaactaagt ttttcttgtg
     3661 ctttcgcatg gtcagaagga tgtttttgct cttccacagg tttagaagga tcgattgcat
     3721 catgttgttc tggaacttta gattctggag ttcctggatg atgaggtgca tgcttttctg
     3781 catcatgttt cttctcttct actccttgtt gaacgttagg gactactgga tctggatgtg
     3841 cagatactgg tgctgttttg ttcttttcat cgttcacacc atttgattgg tttggtgtcg
     3901 ttgggtctgg atgtgcagac actggttttt ggttgttcat tttattgtta ttatttttgt
     3961 tattgttatt catggatgtt tcctcctaag atttaaaata gtgatctaag attttcgttt
     4021 gtttcagata aaaaccgata gcgacgccga tcgctgccat gatcaagatc aataaggtct
     4081 tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa tccaagtacc ccaaagatga
     4141 tagggagctt gtatgtcgaa aatagttctt tcatttttgg tcccccccta ttctactcgt
     4201 ttgcggtcat tgcttcgttt agctggttct tcaaagcctg tgtatttcac cgatactttc
     4261 actggctctt ttgttcccaa tatgtgttgt acttcatttt cgatttcttt catcaatgtt
     4321 cctgttttac caatgagtgc tgaagttcgt ttcaactccc cttttacatt gacggcgatc
     4381 ttgtttttcg ttgctttgac atttactttt ggtgaatgga tgaattcatc ttcatgcaag
     4441 tgagaacgaa ccattccttc aattgctttt ttatccaacg tcaactcccc atcaccatgt
     4501 ttcagtagaa agtttccgat tgtttttgga taaaacagga aaacaagtaa cgtgataatt
     4561 gtcaaaatag caaatgcaac ggcaagccaa aatacatatt gttgcacgta tgaattagtg
     4621 atggaaaaga atcgaaactt ctctagctga aaaggcaatg gataggttgc acttaatagt
     4681 atcgcaaaca aaagcacaga tagcaaaagt aatgttccga tcactccgat cgtttttaca
     4741 cctttattca ttttcgcacc tcctcatagg ggtgaaacga tcaacttctt ataagttgat
     4801 cgcttctcca ccagttactc cgtaaatttg ggctgtaaca taacttgctt tattcgatgc
     4861 taagaaaaca taaactggtg ctaattctcc tggctgacct gcacgttcta gtaaactttg
     4921 ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg tctagttgta atggtgtcca
     4981 gattggacca ggtgctacac catttacacg gattccttta ggtgcaagct gttttgctaa
     5041 accgatcgta aagttagcga ttgaagcttt tgttgctgcg taatctaata aatgttcgct
     5101 tggattgaat gcttgaacag aagttgttgt tacgattgct cctcctgctg ggatatgcgg
     5161 aacagcttct ttcactaaag caaacatagc gatgatgttt atcataaatg tgtctttgac
     5221 ttgctcaagt ggtaactcag caatagactg acaagaaatt tgctgtcctg cgtttagtac
     5281 catcgtatct agcccgccaa gttcactcac ggctttatct atgatttctt taggtgcttg
     5341 ttcgtcacga agatcaaaag gtagtaagac aatcttacgt cctgcaactt tcacgtattt
     5401 tgctacttcc tctgcatctt tttcttctcc tgggaagaat tgaagagcga tatctgcccc
     5461 ttctctagca aatgcaatcg ctgctgcacg accgatccct gaatctccac cagtgattag
     5521 tacacggcgg ttttctagtt gattattgcc ttcataggat tcttctccac aatcaggaac
     5581 aggaatcata ttctgctgta aagctggtgt atcctgatct tgtttaggaa atttctccgt
     5641 atggtacaag tttcgcgggt cgattaattt tggttctgtc attgctgtat tcctcctagt
     5701 ttttaaattg tattgccaaa acaaatttct ttgtttgata cctgttttaa atagattctt
     5761 taatttgtat aatctttccg gtataaatgg taacctaaaa ctactgctgc tatcaaagag
     5821 ccaaagacta cgagtataaa gcctagagca gaacttttac ccggtaatcc tcctgtattc
     5881 attccccaaa tccctgcgaa caacgccgga acggaaatga ttaatgcagc tgaatctaga
     5941 tatttcatga tatgatttag attattgtcc atcatcccgt tgaatagatc accaatactc
     6001 tctaataaat ctcgatagat ctcgatcatt ttttctgttt gtcgctgtct caaatggaca
     6061 tcatagagta gctgttcatc gtctaaacgt ttgatgaaag tttcagaatt ccataattga
     6121 tccattgttt cccgttgatc tcttaaggta tgatcgatat atacgatgtc tctttcaaga
     6181 tctgcctgtt tatacagttc ttcattttcc gttgttttac gagctgcttt atccaaagcg
     6241 tcaattcgct ttttcatctc tcttagttct ttcacgtaat gggtgtaaat atctaataat
     6301 gtatacgtga tgatcttttc aaacgagttt aaatgatttc catgctttgt cagcagccga
     6361 tccacaaatt cggtttttct atcgatacaa gtaatcaaga gattatcgga aagtacaaaa
     6421 gatattggcg ttaatttttt ttcgatacga ttatctgact gatctagatt aatcactacc
     6481 aaagaaaatg gatgctctag tttcgtctca taaaggcgct ccatccgaga aacttcttcc
     6541 ggatattcgg ttcctacgaa aatatcatca ggaagctcga attcttcgat tgcttgctca
     6601 atctcttcgg gctttgcact atcaagtacg agccaattat attttttttc atctgtttct
     6661 attacattct tttcttcatc cacaaagtag tatctcaatg tatacctcct ttttggactt
     6721 tctcgaaact attagctgaa gaagcagttc tttaatttga tattataatc gagtactgcg
     6781 atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga agtccatgtt caatcgttta
     6841 ccgaaataat tttgctctaa tttagatgta tagatgtcca ataatggatg ctcgatttta
     6901 gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt gaaaaaagct gccttttgca
     6961 aaagcacctt tgtcccttag aaatggatcg gaatcattga taagcgcgag tgcgattttt
     7021 gtattttccc caaccttttc aattaacgtc tctactgcat ttttagacat tgtaatttcc
     7081 attcctactc cccctcttca gttgttgtct tctattattt aacaactaca tctgtttttg
     7141 gttgaacttt tgcatatttt ggatatttag ggaatttagc tacacaacgg aaagccgtca
     7201 tccctgcata tttttcgtaa tatttcttcg ttggcccttc aaaatccttg ctccacggtt
     7261 tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg agtttcttcg tattcacgtt
     7321 cacctgctgc tgttggatgt ttttttgctt ttgccatgat atagccttgt gcattccaac
     7381 gcggatgaag tggtaaccaa cggtcatgta agatagcatt taatgcatct tggtcatgga
     7441 atcgtaattt ttctggatta tgttcgatga aatctaatac tttttgcgta atattttcat
     7501 ctaaccattt tttcacattg atcagcatca aacctgagtt gaaataacgc attgatttag
     7561 ctgaaatttt cattttttcc agtcgttgat ggaacccagc gtcttcaaca gctgctacga
     7621 ttgaatcacc gaagtcaaga cgccataatt tagagatatc ttgtaaagcg atcatgtcgc
     7681 aatccatata taagatacgt tcaacttctg tgccgcggaa caattccgga atcgcaatac
     7741 gataataagc tgtttctgga atacgatcgc tgattaccac atttgtaaag aattctttat
     7801 tgattttcaa gaattccaca tctgagttca ttcgtgcgtt tttcactgag aagcgtaaaa
     7861 gctgtttact ttcaaaatcg atatcatcgt caatcacata gaaccgaaca aatttagacg
     7921 ggttgcaatt atctaatact gagacaaata atgcagctaa atgtggtacg aattttgtgt
     7981 tacaacttgc gactactgct atttcttttt tattcattga tctcgcctac tttcgttaga
     8041 gtactgtgtg ctaatttttt taagtataaa ttggtatacg gatgatcttt caacgtattc
     8101 caaggtttat catgtcctgt aaagtgaacg atcgaaggtt tttcatttcc ttctttatac
     8161 tgacgttcat atttttcatt tggtgctgga tgacgttcaa aaatcagcga agtctgcatg
     8221 ttccattttg gatgaagttg ttcccaatct tcataaagta cagcattcaa agcatcttgg
     8281 tcgtgataga tgattcgatc gccattttca ctgaggtaat ggatagtttt ttctgtaatt
     8341 tcttttttat tccattgatc aatgtcaatc accatcactc ctgaattgaa gtacaagtca
     8401 tcggaatcga ttcctaatct ttccaaagct ttcacttggc ctggatcgat aatagcgccg
     8461 attgtttttc catttaacgg ctcatcatat agttttacga tatcgtctag tactaacaca
     8521 tctgaatcta agtaaaggac tttcttataa tcctctttag ctaataagtt tggtaaagaa
     8581 attcttaagt aagcggtagt tgtgatatgg tcactgacta ggaagtcttc gtagatatct
     8641 ttttccactg tcaggaattg gatacttgca ttacttgaat atttatttac cgtttcttcc
     8701 aatccttctt tactatattc tgatagacca tcgtcaatca cataaaattt tatgcgtctt
     8761 gttttattgc aattttctaa tgcagttgcg atcatcacgc tcaagtaagg agcataattt
     8821 tcgtctgaag cagttaccac aggaactacg ccgtatctct tctccatcaa aatcactcct
     8881 ttttctaatc tttttctaac ctgttctaca tctagaatat tcctcatttg gttttaaagc
     8941 aaaatatatg ctttgttttc attgaatatt ttaaaatttt tttatcacaa aacgcatcgg
     9001 taagcgctaa ctattcgttt ttcatggctt tttattcaaa ggtattgacc aaaaactact
     9061 atatatagcc acctttttct taagaatgtt ttttgaaaaa attcataatg acaaagcaag
     9121 ttagtttcat ttttttaaat tctcatttaa tcttaaggta acaggaaaat agatatgtaa
     9181 aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt accttgcttg
     9241 atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt gatgaaaatg
     9301 gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat attttttccg
     9361 aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct gaaatgaagg
     9421 atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca ctgagaaaag
     9481 ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa acccaagatg
     9541 atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat gaacatttca
     9601 atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct tctcttagct
     9661 atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta caaagagatc
     9721 tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa atcacgccta
     9781 caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga atcgaatcga
     9841 gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat tacagcgggt
     9901 tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac caagtaacac
     9961 tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa aaagaataaa
    10021 atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg caaaatcata
    10081 ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca gaacgtattg
    10141 ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca cgggatatac
    10201 cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc cctcttcttt
    10261 ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca cctgaaactt
    10321 tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag atcgaagtag
    10381 tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag agtgcttttc
    10441 ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac ggagaatccg
    10501 ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa tatctatctt
    10561 catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag tcctacgtgc
    10621 tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca ggctcacgac
    10681 ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca aacggcaaaa
    10741 tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc tgatttcaca
    10801 acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac agccaagtaa
    10861 aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
    10921 acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat gagccgtctg
    10981 ctctaaccaa ctgagctaaa ggtacatcat aaataaaatt tatttttgtt acttttcaac
    11041 tatagcacga atgacaaact ctcacaagtt tcaaaatata agtaaaataa tattgttatt
    11101 cttatttctt ttacttgtgt cataactatt ttgttacttt aatttttatt tgcttatttt
    11161 agaaaaaatg ttattctttt atttattctc aatgttagga gtgattattt tgccatttat
    11221 tgcgatttta ttcgatttag ttgcggcagc agcttatttt ctccaattaa attatcaaac
    11281 ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg attacccttt tgcttcttgt
    11341 catgatgctc agctataaag gaaaaagata tgcccgtatt caaacaaaag tatttgttaa
    11401 atatgtttcc attcgctatg ggatcatcat tctttccttt ttagtgaatg cgatcgtcct
    11461 ctttttatat gtattgaatt acttagatat caatccattg attttttctc gatagataag
    11521 acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt tttcattttt
    11581 cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg caataacatg
    11641 ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg gtgagagttt
    11701 tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg aattggaaca
    11761 aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg tggaaagttt
    11821 ttaattacaa ctactttgaa cccgatagtg tttttataga agaattatca ggactggttt
    11881 tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat tgagtactat
    11941 tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg aaaggtgatt
    12001 aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg aatttcttaa
    12061 gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt cctttattca
    12121 aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta atctatatcg
    12181 ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca gcaagcaaac
    12241 tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa ggttaacaat
    12301 ctatatataa aggagacatt tctatgcgat tatggcatga aaaattgatt tcaaagcttc
    12361 ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga aatggctggg
    12421 gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga ttattccaat
    12481 atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca ccagagtgga
    12541 aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct actgtagagc
    12601 gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt ttagcaaatt
    12661 tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta tctcctctta
    12721 cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt tttagggtta
    12781 tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc gccaacttcc
    12841 ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc gacgtctgta
    12901 tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct gttgcttctt
    12961 ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa tattcccatg
    13021 catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa ctcctctgtc
    13081 gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga acaacctgcc
    13141 attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt ccccccctga
    13201 aaatagacta aaataataac gctttctttt tttgttttca gacgaaaatt aataatttct
    13261 aaatattctt taaaaaagct tataactatt atgccttttt taatatataa cacagttatt
    13321 tttaaaaata agaatgctaa atcactctac aataacggcg aaagcacaaa aaataaaaaa
    13381 attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct cattctttca
    13441 tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt
    13501 ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa
    13561 aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac
    13621 agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt
    13681 ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg
    13741 tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca
    13801 gcccattcaa cgaccaatga attttt
//
LOCUS       DANJMA010000059        13809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000059 DANJMA010000000
VERSION     DANJMA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PWU31_002213"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PWU31_002213"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL1084706.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="PWU31_002214"
     CDS             complement(2262..2513)
                     /locus_tag="PWU31_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL1084707.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="PWU31_002215"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="PWU31_002215"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL1084708.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="PWU31_002216"
     CDS             3463..3681
                     /locus_tag="PWU31_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084709.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="PWU31_002217"
     CDS             complement(3970..4482)
                     /locus_tag="PWU31_002217"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL1084710.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PWU31_002218"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PWU31_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL1084711.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="PWU31_002219"
     CDS             complement(6935..7378)
                     /locus_tag="PWU31_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL1084712.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="PWU31_002220"
     CDS             complement(7379..8167)
                     /locus_tag="PWU31_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1084713.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="PWU31_002221"
     CDS             complement(8164..9105)
                     /locus_tag="PWU31_002221"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL1084714.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="PWU31_002222"
     CDS             complement(9282..9869)
                     /locus_tag="PWU31_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL1084715.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PWU31_002223"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PWU31_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL1084716.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="PWU31_002224"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="PWU31_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1084717.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="PWU31_002225"
     CDS             complement(12786..13721)
                     /locus_tag="PWU31_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084718.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DANJMA010000060        13603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000060 DANJMA010000000
VERSION     DANJMA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(117..1247)
                     /locus_tag="PWU31_002226"
     CDS             complement(117..1247)
                     /locus_tag="PWU31_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1084719.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1788..1994)
                     /locus_tag="PWU31_002227"
     CDS             complement(1788..1994)
                     /locus_tag="PWU31_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084720.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2010..2624)
                     /locus_tag="PWU31_002228"
     CDS             complement(2010..2624)
                     /locus_tag="PWU31_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL1084721.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDKVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2771..3454)
                     /locus_tag="PWU31_002229"
     CDS             complement(2771..3454)
                     /locus_tag="PWU31_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1084722.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3563..4021)
                     /locus_tag="PWU31_002230"
     CDS             complement(3563..4021)
                     /locus_tag="PWU31_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL1084723.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4105..5868)
                     /locus_tag="PWU31_002231"
     CDS             complement(4105..5868)
                     /locus_tag="PWU31_002231"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL1084724.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6089..7549)
                     /locus_tag="PWU31_002232"
     CDS             complement(6089..7549)
                     /locus_tag="PWU31_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084725.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="PWU31_002233"
     CDS             complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="PWU31_002233"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL1084726.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8706..10133)
                     /locus_tag="PWU31_002234"
     CDS             complement(8706..10133)
                     /locus_tag="PWU31_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1084727.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10164..11378)
                     /locus_tag="PWU31_002235"
     CDS             complement(10164..11378)
                     /locus_tag="PWU31_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL1084728.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(11719..11961)
                     /locus_tag="PWU31_002236"
     CDS             complement(11719..11961)
                     /locus_tag="PWU31_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1084729.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11993..12550)
                     /locus_tag="PWU31_002237"
     CDS             complement(11993..12550)
                     /locus_tag="PWU31_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1084730.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12563..12751)
                     /locus_tag="PWU31_002238"
     CDS             complement(12563..12751)
                     /locus_tag="PWU31_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL1084731.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12764..13330)
                     /gene="amaP"
                     /locus_tag="PWU31_002239"
     CDS             complement(12764..13330)
                     /gene="amaP"
                     /locus_tag="PWU31_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL1084732.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4067 a         2810 c         2219 g         4507 t
ORIGIN      
        1 atatgatttt ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg
       61 aaaaataaaa gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat
      121 tgtttttcag gtcgatagag cgccaactct tcggcctgta tctttgaaga aatattcaac
      181 gtgattccga ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc
      241 aatggcacac cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga
      301 acgaatagca aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga
      361 ctgcttaaac aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca
      421 ccgattagcc caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga
      481 agataccctt tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat
      541 agtgcataaa acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct
      601 cttaaagtcg cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt
      661 cctaaatata ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga
      721 tagatccctt ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg
      781 atcatcaatg ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta
      841 atcaggaatg gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt
      901 aaataaaaaa tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga
      961 gagatccaac gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact
     1021 aaaaagaaaa tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt
     1081 agtttgactg aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga
     1141 cgcaataata gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg
     1201 tatacttcta aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg
     1261 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     1321 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     1381 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     1441 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     1501 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     1561 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     1621 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     1681 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     1741 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     1801 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     1861 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     1921 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     1981 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     2041 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     2101 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     2161 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     2221 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     2281 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     2341 tttacttctt ctactttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     2401 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     2461 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     2521 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     2581 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     2641 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     2701 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     2761 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     2821 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     2881 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     2941 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     3001 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     3061 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     3121 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     3181 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     3241 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     3301 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     3361 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     3421 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     3481 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     3541 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     3601 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     3661 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     3721 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     3781 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     3841 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     3901 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     3961 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     4021 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     4081 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     4141 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     4201 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     4261 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     4321 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     4381 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     4441 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     4501 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     4561 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     4621 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     4681 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     4741 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     4801 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     4861 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     4921 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     4981 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     5041 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     5101 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     5161 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     5221 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     5281 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     5341 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     5401 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     5461 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     5521 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     5581 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     5641 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     5701 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     5761 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     5821 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     5881 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     5941 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
     6001 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
     6061 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
     6121 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
     6181 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
     6241 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
     6301 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
     6361 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
     6421 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
     6481 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
     6541 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
     6601 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
     6661 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
     6721 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
     6781 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
     6841 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
     6901 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
     6961 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
     7021 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
     7081 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
     7141 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
     7201 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
     7261 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
     7321 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
     7381 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
     7441 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
     7501 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
     7561 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
     7621 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
     7681 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
     7741 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
     7801 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
     7861 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
     7921 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
     7981 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
     8041 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
     8101 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
     8161 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
     8221 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
     8281 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
     8341 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
     8401 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
     8461 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
     8521 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
     8581 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
     8641 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
     8701 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
     8761 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
     8821 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
     8881 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
     8941 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
     9001 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
     9061 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
     9121 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
     9181 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
     9241 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
     9301 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
     9361 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
     9421 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
     9481 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
     9541 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
     9601 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
     9661 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
     9721 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
     9781 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
     9841 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
     9901 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
     9961 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    10021 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    10081 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    10141 cctcttttct attttaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    10201 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    10261 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    10321 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    10381 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    10441 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    10501 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    10561 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    10621 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    10681 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    10741 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    10801 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    10861 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    10921 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    10981 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    11041 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    11101 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    11161 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    11221 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    11281 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    11341 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    11401 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    11461 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    11521 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    11581 tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat ataaaatata
    11641 taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca tttcaacata
    11701 agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta atactaaaat
    11761 gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc cccaagttcc
    11821 tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg cgatgatccc
    11881 cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac cgccgacgat
    11941 taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact tcttattgta
    12001 cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca gattttgctt
    12061 tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt actttgtctt
    12121 gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca acattcacat
    12181 tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc aattctttga
    12241 tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct agatccacag
    12301 ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct gtgttgacta
    12361 atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca tctacatctt
    12421 tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat tcaccattca
    12481 tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta ttcatgtttt
    12541 tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt cctgactgtt
    12601 tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac aatgtcttga
    12661 aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca aagataatag
    12721 ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc actcgagagt
    12781 gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact gccactttga
    12841 ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc atcaatacac
    12901 ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg atatggattc
    12961 gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct tcgtgtaaat
    13021 gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca tcttcccgtt
    13081 tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag atgataatag
    13141 caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag taattcgtta
    13201 aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca ttgatcaacg
    13261 caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt atttttaacc
    13321 ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac tctaccgaca
    13381 atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc taccaaacat
    13441 tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat tgcttactta
    13501 ttctctattt acttttgatt gctggctata cttaaaggat attattcaag attaaaaatt
    13561 gcaaagatta tgctcagtta ttattacaag attctattta ttt
//
LOCUS       DANJMA010000061        13193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000061 DANJMA010000000
VERSION     DANJMA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            157..1896
                     /gene="yfcC"
                     /locus_tag="PWU31_002240"
     CDS             157..1896
                     /gene="yfcC"
                     /locus_tag="PWU31_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL1084733.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2053..3237
                     /gene="iadA"
                     /locus_tag="PWU31_002241"
     CDS             2053..3237
                     /gene="iadA"
                     /locus_tag="PWU31_002241"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL1084734.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3348..3551
                     /locus_tag="PWU31_002242"
     CDS             3348..3551
                     /locus_tag="PWU31_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL1084735.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3652..4314)
                     /locus_tag="PWU31_002243"
     CDS             complement(3652..4314)
                     /locus_tag="PWU31_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084736.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4327..5829)
                     /locus_tag="PWU31_002244"
     CDS             complement(4327..5829)
                     /locus_tag="PWU31_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084737.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6377..8056
                     /locus_tag="PWU31_002245"
     CDS             6377..8056
                     /locus_tag="PWU31_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1084738.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8183..8662
                     /locus_tag="PWU31_002246"
     CDS             8183..8662
                     /locus_tag="PWU31_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL1084739.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8881..9561
                     /locus_tag="PWU31_002247"
     CDS             8881..9561
                     /locus_tag="PWU31_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL1084740.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9569..10900
                     /locus_tag="PWU31_002248"
     CDS             9569..10900
                     /locus_tag="PWU31_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1084741.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10915..11877
                     /locus_tag="PWU31_002249"
     CDS             10915..11877
                     /locus_tag="PWU31_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL1084742.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11988..12326
                     /locus_tag="PWU31_002250"
     CDS             11988..12326
                     /locus_tag="PWU31_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL1084743.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12405..13112
                     /locus_tag="PWU31_002251"
     CDS             12405..13112
                     /locus_tag="PWU31_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL1084744.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4207 a         2323 c         2889 g         3774 t
ORIGIN      
        1 aaaaaataat catacatagg agaaaaaaag caaaatagaa aaaagtgatt ttctaataaa
       61 ttattcatgt ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc
      121 atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga
      181 aaaccgaaac gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt
      241 gtagtcatct ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa
      301 gatattgaat acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat
      361 tccttccgtt atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg
      421 caagatcatc cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc
      481 attgctgacg gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat
      541 gatgtgcttt acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa
      601 acagccaaaa tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt
      661 gaaggactag tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt
      721 gttgttggag gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat
      781 gcgtttatca gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct
      841 ttttcattcg gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg
      901 atggttccat ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat
      961 gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct
     1021 caagggattg ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta
     1081 gttgttactg cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa
     1141 ccagaaagtt cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca
     1201 tctgatgaaa ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt
     1261 gtccttgtct ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct
     1321 tctcaattct ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat
     1381 ggaatgaaag tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt
     1441 acagcaatcg tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca
     1501 tctgttcctt cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt
     1561 ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt
     1621 acatcaaatt caggacaagc agctttgact atgccaatca tggctccact ggcagattta
     1681 gtaggtgtat ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac
     1741 gcttttacac ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg
     1801 gcaaaatggg caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt
     1861 ttcatcatga ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa
     1921 gtaatccaaa cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag
     1981 taaagatcct atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa
     2041 gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga
     2101 aaaatggatg tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc
     2161 ggctacgaag gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt
     2221 ttcattgact gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga
     2281 acgccagaag tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt
     2341 ttgggaacag acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg
     2401 gaagcagaag gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca
     2461 gtaacagatt ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa
     2521 atcgcagtat ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta
     2581 gcagatgctc gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa tgtccactta
     2641 ggcggaggaa aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg
     2701 atcacacaat ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg
     2761 gcatttgcca aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg
     2821 gagaagacag atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat
     2881 gtttctttag ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat
     2941 gaaaacaatc atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc
     3001 aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt
     3061 aatccagcac gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca
     3121 gatctgtgta tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa
     3181 ataatggtcc aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca
     3241 acagctctaa ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat
     3301 gttaccctga aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa
     3361 aaggacgttc taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca
     3421 aagcaacaga cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca
     3481 tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg
     3541 aaaaaaaata aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata
     3601 tctgcgagac agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc
     3661 gattttttga aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc
     3721 aatctcatga gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag
     3781 tttgacgata tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat
     3841 tagttgatgt tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga
     3901 aagtttggtc acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc
     3961 taaagcaact tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac
     4021 gttgtctaga gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc
     4081 atttttacga taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt
     4141 tccttctttt ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc
     4201 acttgctcct aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata
     4261 aagcgcatct tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt
     4321 cctcctttag tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa
     4381 aaagacagaa aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg
     4441 tttgacattg atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt
     4501 tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc
     4561 gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc
     4621 cacggcattc gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca
     4681 aatagccact tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag
     4741 caagacgcct aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat
     4801 gataataccg gctacagtca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt
     4861 taaaggttcc aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt
     4921 gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt
     4981 gtaaatagca gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt
     5041 gtaagaagtg taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc
     5101 gcttgtttcg ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc
     5161 ttcagtactc gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt
     5221 agaatcaatg actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt
     5281 gatttttgct gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat
     5341 ttgaaagtct gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc
     5401 tgcatcagta gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc
     5461 atcttttgcc atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc
     5521 agctattttt tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg
     5581 acgttcttca gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc
     5641 tcgattggca cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc
     5701 ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac
     5761 ggcaaacaat aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat
     5821 aaaattcata ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa
     5881 agctagctta aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa
     5941 agtcactata gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg
     6001 aagaagaaat gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg
     6061 tgcatagttg ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat
     6121 ggggaacagc tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt
     6181 ttgttaatga agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct
     6241 ggtaattttc ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt
     6301 ggaatggctc atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata
     6361 agaagaggtg aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa
     6421 tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct
     6481 aaaatatcct gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt
     6541 agaagaaaac attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat
     6601 cggggcactt ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac
     6661 gatcgagtta gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt
     6721 ccatgtggta gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg
     6781 taccactcat acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat
     6841 cgtttatacg ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta
     6901 tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa
     6961 tgcagaaaat cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac
     7021 gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt
     7081 gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga
     7141 tttcgaacga attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga
     7201 tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga
     7261 aactggccga atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg
     7321 taatgtcaag attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat
     7381 ggaaaccatc gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt
     7441 gatctctgag aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat
     7501 caatttgatg aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc
     7561 tcatgcagat ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg
     7621 cggagacgtg ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga
     7681 aaatgtaatg attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg
     7741 aaaaatcttg tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa
     7801 gaaaatcatt tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg
     7861 tgatctgatg aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag
     7921 caatgatttt gagtggagca aattaaagca agacatccga gatcaattag gccgttactt
     7981 atttgaacaa acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg
     8041 tcgaaataaa aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc
     8101 tctttttttg cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg
     8161 atagaataaa gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga
     8221 acaaaagatc agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga
     8281 agaactacaa aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg
     8341 aaacaaaact ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa
     8401 aacgagacaa gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat
     8461 gcatacagga tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga
     8521 ttattgtttt ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg
     8581 tgaaatcggg agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat
     8641 ttacgctgat ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa
     8701 acttgagatt atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt
     8761 gtacatactg tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat
     8821 gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat
     8881 atgaaaatta cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc
     8941 gtgatgattg acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta
     9001 aagacagatt ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct
     9061 attgcaaaag aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac
     9121 agcagaggag tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc
     9181 gtggtaaaat tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag
     9241 tatatgggcg agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca
     9301 ggggatactg cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc
     9361 gctttcttgc cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg
     9421 caaacaattc gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa
     9481 caagatcccg aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc
     9541 tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata
     9601 tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa
     9661 tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc
     9721 aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg
     9781 tttgacattt ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg
     9841 attagacaag gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg
     9901 ctatattacg ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc
     9961 attggaagat ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc
    10021 aaagctagct gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt
    10081 cgcaaccatg atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt
    10141 cagtgatatc tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc
    10201 tgattatcag cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca
    10261 tcatcgtttg atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat
    10321 catcgatcgg gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc
    10381 tgttagccat caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt
    10441 gacattggaa ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg
    10501 acaaccgcag attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga
    10561 tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca
    10621 aatggtcaat ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt
    10681 cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct
    10741 acattatttg cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga
    10801 aatcggcaga cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc
    10861 caaagcaatc gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca
    10921 gtacagcaag aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat
    10981 ttgcgccctg atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa
    11041 agtttcccag aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta
    11101 gcagaaatgc aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat
    11161 acagctaatt cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa
    11221 atcgaccatc atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca
    11281 agtagctgta gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg
    11341 aacgttaagg ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg
    11401 tatccctcca cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt
    11461 gatgcaagtg ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc
    11521 ggttatcttt atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg
    11581 caagaaattc ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt
    11641 cctggagtga ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat
    11701 taccgcgtac gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat
    11761 ggaggaggcc atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa
    11821 atctataaag agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac
    11881 ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct
    11941 ttttccgtta caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa
    12001 attgcccaga atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag
    12061 aatgcgggaa cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg
    12121 tattagatgc aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact
    12181 tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt
    12241 tagtagaagg cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga
    12301 aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta
    12361 ctactaatga taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa
    12421 tgtatggttt tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa
    12481 ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca
    12541 atgtgactgg tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg
    12601 gtgttcaaca aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga
    12661 gtttgtctca agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg
    12721 aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac
    12781 ttgttccagc agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat
    12841 tgcttagctg gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac
    12901 ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt
    12961 tgagtgaagg cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg
    13021 cagcagcact gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga
    13081 tcttattgtt cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt
    13141 gatctgagct aataccgaga gttagctgat tgaataccat ttatttggaa taa
//
LOCUS       DANJMA010000062        13085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000062 DANJMA010000000
VERSION     DANJMA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(79..2199)
                     /locus_tag="PWU31_002252"
     CDS             complement(79..2199)
                     /locus_tag="PWU31_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1084745.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="PWU31_002253"
     CDS             2429..3607
                     /locus_tag="PWU31_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL1084746.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="PWU31_002254"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="PWU31_002254"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL1084747.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="PWU31_002255"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="PWU31_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL1084748.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="PWU31_002256"
     CDS             complement(4960..6213)
                     /locus_tag="PWU31_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1084749.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="PWU31_002257"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="PWU31_002257"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL1084750.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="PWU31_002258"
     CDS             7790..9136
                     /locus_tag="PWU31_002258"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL1084751.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="PWU31_002259"
     CDS             complement(9444..9830)
                     /locus_tag="PWU31_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1084752.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="PWU31_002260"
     CDS             complement(10098..10247)
                     /locus_tag="PWU31_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084753.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="PWU31_002261"
     CDS             10709..10921
                     /locus_tag="PWU31_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084754.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="PWU31_002262"
     CDS             complement(11199..12998)
                     /locus_tag="PWU31_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1084755.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DANJMA010000063        12748 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000063 DANJMA010000000
VERSION     DANJMA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..766)
                     /locus_tag="PWU31_002263"
     CDS             complement(<1..766)
                     /locus_tag="PWU31_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1084756.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILE"
     gene            complement(763..1416)
                     /locus_tag="PWU31_002264"
     CDS             complement(763..1416)
                     /locus_tag="PWU31_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084757.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1704..2224
                     /locus_tag="PWU31_002265"
                     /pseudo
     CDS             1704..2224
                     /locus_tag="PWU31_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2126..2842)
                     /locus_tag="PWU31_002266"
     CDS             complement(2126..2842)
                     /locus_tag="PWU31_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL1084758.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2892..3101)
                     /locus_tag="PWU31_002267"
     CDS             complement(2892..3101)
                     /locus_tag="PWU31_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084759.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3378..4651
                     /locus_tag="PWU31_002268"
                     /pseudo
     CDS             3378..4651
                     /locus_tag="PWU31_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4758..5789)
                     /locus_tag="PWU31_002269"
     CDS             complement(4758..5789)
                     /locus_tag="PWU31_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1084760.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5796..6626)
                     /locus_tag="PWU31_002270"
     CDS             complement(5796..6626)
                     /locus_tag="PWU31_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1084761.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6623..7438)
                     /locus_tag="PWU31_002271"
     CDS             complement(6623..7438)
                     /locus_tag="PWU31_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1084762.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7435..8259)
                     /locus_tag="PWU31_002272"
     CDS             complement(7435..8259)
                     /locus_tag="PWU31_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL1084763.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8249..9349)
                     /locus_tag="PWU31_002273"
     CDS             complement(8249..9349)
                     /locus_tag="PWU31_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1084764.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9527..10312
                     /locus_tag="PWU31_002274"
     CDS             9527..10312
                     /locus_tag="PWU31_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL1084765.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10345..10728
                     /locus_tag="PWU31_002275"
     CDS             10345..10728
                     /locus_tag="PWU31_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL1084766.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10804..10935
                     /locus_tag="PWU31_002276"
     CDS             10804..10935
                     /locus_tag="PWU31_002276"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1084767.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10904..11140
                     /locus_tag="PWU31_002277"
     CDS             10904..11140
                     /locus_tag="PWU31_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1084768.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11218..12189)
                     /locus_tag="PWU31_002278"
     CDS             complement(11218..12189)
                     /locus_tag="PWU31_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL1084769.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3844 a         2826 c         2273 g         3805 t
ORIGIN      
        1 tttccagtat tattttaaga ctagcaagag gggttttaag ttcatgggat gcacctttaa
       61 aaaagtcata ttttaacttt tctaatttta aaatttcttt atttttaaat tccaaatcgt
      121 caattgttct taataaagta gaatacaaat cattaatttg ttgttttaat tctccaactt
      181 catcattaga gctaactttt aaactcattt ttttatcaag tttcatcatt ttatcagtaa
      241 caatttttat ttcttgtata ttatttttta ttgattttgc ataaattaaa gaaataatag
      301 cagaaaataa aattgatatt aataaagaat atggtaaaaa tttaaggctt aaatctttcg
      361 catctttttg catatcagct gtagaaacaa attgtagatt tgttttttta ccatcattaa
      421 gctttatttc tctttcttca attatcaaag aattactatc actttctaaa ttaacattta
      481 tattatcttt gatttgtatt tcattcttat tatttttttc ttttatgaat acttttattt
      541 cactactctt ggaataaaac tccaaagttt gttctatata ttttatttct tttccattca
      601 tattactaga aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg
      661 ttttaggaaa gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag
      721 aaaatatttg tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt
      781 atatcctaca tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt
      841 tatatataca tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc
      901 aataatttgc attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc
      961 tagttctttt gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa
     1021 attaaccgat agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa
     1081 agaatctatt ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac
     1141 taaattagta aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat
     1201 tggtaaattg cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat
     1261 ctgaatgtct aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc
     1321 atctttagct tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct
     1381 tatcatatta tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac
     1441 actagatatt atacagcata ttatggctct ataatatcta gtgttggtga cgccttctgt
     1501 tgccaatact ttttcttttt tttaataaat gcaaacagac accttgaaat cctttagaaa
     1561 aaaggcttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt
     1621 tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat
     1681 caccctacca aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag
     1741 ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag
     1801 cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga
     1861 ttcgtattat ccgcaacggt tcctatacga cacgtactca gatgttgaaa gtcaaagaaa
     1921 agttgactat tttagaatta aaacgtaccc gttttctatg ctatgattgt gggcagacct
     1981 tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaagcagg
     2041 cgattctcat agaactgtac gaaaatcaat cacgtaatct gaaaatcaga agaagtaccg
     2101 tcggctcaag cgatattgga aactcttact caaagattcc acgacattgg agccgctcaa
     2161 acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg tcgatgaacc
     2221 gttgtgcaca atggtgtcct gaacgtacag cgacaccttc catatccaga gcagtcgcca
     2281 catcacgggg atggatgcca tccaaattaa aagcaaccac gccagtatga tgttcaggat
     2341 cttgcggtcc gtaaatggtg ataccgccga tcgcttgtaa tttaggtaaa acgtaggcaa
     2401 ccagttcttg ctcataacga tgaatattct ccataccaat ctcattcaag taatcgattg
     2461 ctcctgcaat gttcggagta ccagcttcaa atttccatgg cagctctttc catgaactat
     2521 cgtaaagatt gacaaaatct atcatttcac cgccgaattc gactggctcc atttgttcta
     2581 acaattgacg tttgccatat aatacaccga ttcctgtcgg cccgcacatt ttatgcccgc
     2641 taaaggcgaa aaaatctgca tccaacgctt gtacatcaac tgtcatatgc ggtgctgctt
     2701 gggcaccgac tggactatcc tcaactacaa cagcctgttc aaccctcagc cgtttcaagc
     2761 acaacaaata accttctgga tctggtttgc cgacctgaag atcatcgatc gtcacaaagg
     2821 aatcaatttt ttccttaatc atcggatgtg cttcaaaaaa atagtctagc tcccgcagtt
     2881 cagaattgag actactccga ttttccagtg ctgctgatcc gctgctcaaa atgttcagca
     2941 tccactggtc tcccaaccgc ttgacttatc ccttcataaa aggacagttc tgctgccgtc
     3001 ccaacacctt gttgatagtc tgcaaaagga attttatagc cgaactgctc cagtaaatag
     3061 gcttcagtaa atagtgatga atactctcgt aaaacatcca ttcactgtca atcatcgtcc
     3121 catcaaaatc aaaaataagt gctttggtca tcttctttcc cccttaccca aaacaagcaa
     3181 aagctgtgtc ctgctctctc tacagtatca gcataaaaac tgccgtctga aaagtcaaag
     3241 gtgaaagaaa tgtggcgcta taatttctag gactgatagt aaatcattct atcagtcctt
     3301 ttgagcataa aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa
     3361 tcaaaggagc gttctgtatg cctgatcttc atcttatcaa actattacta ggaatcaaag
     3421 atggaaacat tcaaatcact catgtcgaga agaaaaaaat acattcaatc aacagtatcg
     3481 ttgtttctgc cacgctcatt aaaacgattc agccctgtcc tcagtgtaaa caggtgaatc
     3541 aacatgggct gatcgttaag aacggaaaga aaaaatcgct ggtccttctc aataagtgtg
     3601 cgaatcaacg gacttatctt gctttggcaa agcaacggta cctctgtcgt ggctgtcaca
     3661 cctattttac ggcgaatacc tacattgttg aaaaaaactg ttttatcgcc aaacaagtac
     3721 attataaaat attggaggaa ttgactgaaa aacaagcaat gacgacgatt tctaaacatt
     3781 gcggcgtttc ttggtcaacc gtttcgcgaa cactcgcttc tctgctcccc atgacaaacg
     3841 tgaaaataaa ttggttgcct cgctgttcat tggtagatga atttcgttca ttgaaaaatc
     3901 aagtcggtcc ctattctttt tcttgtatgg atggcgatac tgggaaatta ctggatattc
     3961 ttccttcacg taagaagaaa gatctcattt cttattttat gcaatttgaa cgacgtgcac
     4021 gtttaaacgt aaaaatactc gtaacagata tgaacgcctc ttatgctttg ctgataaaag
     4081 agtgctttcc tcatgccaaa ctcgttgtgg atcgctttca catcgtcaag cacctcattc
     4141 gcagttttaa ggatatttga ctatgagtca tgaaaagctt cgaccgaaac gatccaatac
     4201 aagccaaaca ctatcgacaa gtgaaggctt tatctcgact tctgatcaaa cgacaggaca
     4261 cgcttgttta tgataaatgg acaaaatggc gtaattttgg ttgggcctat ttaaccgaaa
     4321 gtgaagtcgt agaacgtcta ctttctactt ctgatgaact acgaatagct tatgcttact
     4381 atcaagaaat tcttcaagct ttttatgaca aagaagcgga catcttcttt cagcttgtaa
     4441 aaacaatgcc caagtcggtt ccaagagaac tgcatcatat aaaaaaagcg tttataaact
     4501 atgaatccgg cattcgttta gccttagagt tgccttactc aaatggcaaa atagaaaatc
     4561 tgcataccca catcaaagca ttgaaacgaa tagcttacgg gttaggatga gaaccagaat
     4621 tttcttaatc aatcatctga tcacttatta aacataaaaa aacatctaaa gcagaaaatc
     4681 aattctacct tagatgttct tatatcactc tatcagtcct atttgacaaa gagccagaaa
     4741 tgtttgccga ctatacacta gctttgttgt gcaaaatgct gagtatcaat ttccacaggg
     4801 attgctagaa tatctttcag ctgaatgtat aaggcatcgc taattttctc ttcccgatcg
     4861 ttcaaccgat ccactaaaat ttctttgccc tccagcgttt gaaaaacata ccgttgaatg
     4921 ttgcctaaaa taaatgattc cttccataga accggaatct taaccccctg aggaaccggt
     4981 tcaaattgaa tcagctccgg tcttaaataa taaatgcagc gattgccctt agcggaaaac
     5041 tcgtgggtat actgattcag ctcataccat tcgaaacgat tatagttgcc gataaattcc
     5101 gcaacaaatc ggctctgagg attctgatac aattccttcg aggtggaaac ctgagccacc
     5161 cgcccagctt ccatcacaaa aatccgatca ctgattcgca tcgcctcttc ttggtcatga
     5221 gtcacaaaaa tcatcgtcat ccctagcgat tgctggatag cgcgcaaatc ccgctgcagc
     5281 tgttttctaa ttcgggcatc caaagcactt aatggctcat ccagcagcag gactttcggt
     5341 tcagtgacca atgagcgagc cagtgccacc cgttgtcgtt gaccgccaga aagaaactgg
     5401 acatttttat ctgcctgctc cgtcatatcc accaatgata gcatctcaaa aaccttttcc
     5461 tgaatcacct caggactcag cttctttacc ttcaaaccaa aggctacatt ttcaaaaaca
     5521 gtcatagttg gaaacaatgc atactgttga aaaacaaagc cgatattgcg atccttcgta
     5581 ggcttatcgg caatattctc attgtccagc acaatctcac cgctctttgg ttgttccaat
     5641 cccgtgatac agcgcagcaa cgtagacttt ccgcacccgc tgggacctag taaagtaacc
     5701 agctcaccct tttcaatctg aatttcatca atagctaaaa tctgtttttc atcaaaaaac
     5761 agattgagat catgtatttc taaaaacatt ttccgtcaca actcctttaa gggcttcttt
     5821 ttcgactgct tctcttccct cgtctgctta agggacagct gttgcttttg tcgataagtc
     5881 agcataaagg tagttaaagc aattcccacc ataaacagca aatacaaaac catcacagcg
     5941 ctagccaaat ggccgttttc gttcatccgc cgcagcatat agatgcgtac cgtctgaaat
     6001 cgaccgccaa tcaacaggtt cgtcagcatg tactccccaa aaaccgaaga aaaaatcatc
     6061 agagaaacta gggtcacacc gatacggatg tttggaaaca gcacctggat aataatcgta
     6121 gacatcgggg cgcccaaggt ttctgctgca tccactaatt ccttcagatt aattaagcgc
     6181 atctggttgt taagactaag atagacaatc ggcaacgccg taataaacaa tgcacctacc
     6241 aacactacaa acattgggat accagtttca gcatacgttc gaagcaaagc cgttaccaaa
     6301 atcactcctg gtaaagcata cggcagcagg agcagctttt ccagccaacg attcaaacga
     6361 gggaaataca aatgaatcca aataatggtg ggaatcatca acaacagaat cgtcaccaag
     6421 accacacctg ctaataaaag tgagcgaccc accgcagcaa taaattccct gtccgtcagc
     6481 agctgttgga accattgcaa ggtaaaatca ctaggtaaaa gcgattttga ccatttagtg
     6541 gagctggcat acaaaatcgt tccaataatc ggcagcaagt aatacaggac aaagcagaac
     6601 aaaatcggtt ttattaactt tttcacgtct gcaccacctt gcctttcttt tgaataatca
     6661 acattgccgt taccagcagg acacctaaag cgactgccaa cgcactgcct aaattaggct
     6721 tggcgaacac atctcccgac actaaagccg caattcgaat cgttaaaaga tttacattac
     6781 tgctggtcag ggcatacgct gtttcatagg tccccatgcc attagcaaat aagatgacaa
     6841 aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa gcatacgctg
     6901 atgaagcgcc cagcaattgt gcggactcca gccattcctt tttcacttcc ttcatgctgg
     6961 gatacaaaaa caagacccct aacggaattt ggaaataggt atacgtgatg gtcaatcccc
     7021 accaagaata caaattgaag tccgctagaa atggcaaaat cattttcagc acacctacat
     7081 tccccaacaa aataataaaa gagaatgcca gcggaatccc agcaaaatta gctgccaaat
     7141 tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag attcattaag cataaacata
     7201 aaataaacgt cccgattaac ccaatcacac tggagctgac agcaatcagt acgctgttaa
     7261 caaaggcctg tgtgtaatat ttgttgctga aaatatcact gtaattggcc aatgtccact
     7321 ggttagttaa atcactgtgc aagctgcaca gaaccataaa aagcagcggc agcagtaaaa
     7381 atgcacagaa gataaaggag gcaggaacca gaaccaagcg ttttttcatc tctttcatcc
     7441 cctcatcaat tcttgaatgt cctgcatttt ctctgacggt gcaattccta ataaccagca
     7501 aaacaatgga gccgccagca gctgtggtac agtttgttcc ttcagctttt ggcgtttatt
     7561 tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag ctgccgccat gcagtccgtg
     7621 cgcatccatc ccgtgatccg cagtcaccac gacttgataa cctttttgca gccattcagg
     7681 aaggtaaata gccaataatc tgtccgcctg attaaccgct gctgcatatt catgagaatc
     7741 ggcggtaaat ttgtgaccga catcatcaat attcatcgaa tgtacgacca aaaagtctgg
     7801 ctgatactga ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag ggtaagtgtc
     7861 ctcactataa aagatgccat gctgaatcag tcgctcctcc tgatgctgga tgcgatcaac
     7921 caaacgatta tagggggctt gattgtacaa ctcgctgtac cagtggtaag cggcggctcc
     7981 tgtttttccg ccagcttcct taactaattc aaacagcgac cactctttgg atcgctgagc
     8041 atggtaatta ctatagatgc cattttcata ggctggaacg ccggtcatca gtacttcgta
     8101 taacggacgt gaattgctgg gcagctcact ttttaccttc aataagcttg cttgctggtg
     8161 ctcaactaaa tgattcatga acccaagctg ggcgacggct gtgtcgtagc gacagccgtc
     8221 tagtataact acaatcagtt tgtcaagtct actttgcata ggtcagtacc tccgcctgcc
     8281 aaagctctgg aagctctaca gaggtcttgt tccaagcttc attgtcttca actggcaccg
     8341 ccgcttcata agcatcctcc ggcagtaatt tttcagctac ttctttaggc agctctacct
     8401 tgcgaatagg tcgcgcatag ccctttgcca aattgatttg cccttcatca gataaaatat
     8461 attcccgtgc tagctttgcc gcattgggat gaggggcatt tttattgatt aaggttgtat
     8521 acccgctaat gatggagccg tctgttggca cagttacctc aaaacgctcg gagtcaattt
     8581 ggtcacgata atttagtcca ttgaagtccc aaacaactgc cacatcgatt tcaccttttt
     8641 caagatttgc tacgctggca tcaacggtgg acaaacggcc tttttctgcc atttttttgt
     8701 agtaattcag acctggctga atatcctttt cgtcacctcc gttggcaatc gccgcagcca
     8761 atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact tgatagtcac
     8821 cctcttgcag ctccttccag ctagtcggtg cttgcttcac attctcttta tcggtgataa
     8881 aggaaatggt tcccgtatag cttaacaacc aatccccatc atcatccttt gcccaatccg
     8941 gaatttcctc ccaatagttg gtcttataag gtaaggttaa ctctttgctt tcagccaacg
     9001 gaccgaagct gataccgaca tcaccaatat cggcggttcc attttttcct tctgattcaa
     9061 acttagccaa ttcttctgca ctggacatat ccgtatccac gtgacttaag ccgtattctt
     9121 tttcaaggtc gttccaagtg cctacccaat ttgcccaagt atccggcatt cccaagctgg
     9181 caatctctcc ttcttccttg gctttttcta taatcgtatc taaagagtcg tctgccgcag
     9241 ccgattgctt cgttgcttca cctgagccgc agcctgtcag caggatactt actgttgctg
     9301 cacaaaataa accgatagtc atgttacgaa ttttttcatt cattttcatt cattcttctc
     9361 ccctttcgat aattccattc taacaaagct ttgtaatgag agaatcagtt tcttgaaaag
     9421 gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat tcccgttaac
     9481 cttctaaagc ttttgcggta tgatagtaga aaaaaggagg ctccctatgc agtattatga
     9541 tcagcatctc cacacgtatt tttcctttga ttcagaggaa aaatttgaaa actatttagc
     9601 ctatcagccg gagtttttcg tttctacgga tcactttgat ttaaacaatc catctaccgg
     9661 aaaagatgac attccggatt atccagccta tataaacaaa ctggactctt taaaagaaac
     9721 ctattccacc aaatttctgc gaggtatcga gattggcgtt gttccaggac aagaagagcg
     9781 gatcgtggat tacctcgctc agcatcctta tgatttaaag ctggtaagta ttcatcaaaa
     9841 tggccgcttt gattatatgg acgatgtggt tttaaccaaa gatcccattc aaactaccaa
     9901 ggaatatttt gatcagatgt tgcaggtctt gacggatttt ccagaaggag atattttgac
     9961 tcactttgat tatggattac gccgcttcac cttcacagcg gaagaattcg cggagcattt
    10021 tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg cgatggagtt
    10081 aaatgccaag agcttcttga aatacggcaa cgaagacctt taccgttatg ccgttccttt
    10141 gtatcaaagt gtcggcggaa agctctttac cttaggctcg gatgctcatg ttgccgaaga
    10201 ttatcagctg ggctttaaaa aaatgagtca gctgttggca gactgtcagg tagaaaagtt
    10261 gctagtcatt caaggaaaag aacgccgcct gacgccgctg ccgattttgt gattcctata
    10321 taatagaaga aagtggtgaa aaaaatgagc aacctgtggg actacaatca agaggcaccc
    10381 atccattatt taattgcgcg acattgggat gcgttgaaaa ttgaggcggt ttgccgatcc
    10441 ttgttagcag ccgttccaaa acaacaactg gaaaattttt tggtggctga cagcttgcag
    10501 cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc agcctttgga atatttgcat
    10561 gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga
    10621 cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct
    10681 cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa
    10741 ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt
    10801 tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc
    10861 ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc
    10921 ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga
    10981 agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt
    11041 taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat
    11101 tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt
    11161 ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata ccaagggtta
    11221 gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca
    11281 gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca ctgacttcag
    11341 caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat
    11401 aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg
    11461 aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt
    11521 gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta
    11581 cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag
    11641 cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca
    11701 gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc
    11761 ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac
    11821 atagggagct accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt
    11881 attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag
    11941 tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt
    12001 ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg
    12061 cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata
    12121 aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt
    12181 tccttccata tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac
    12241 ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc
    12301 aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt
    12361 ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac
    12421 tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca
    12481 ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt
    12541 aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga
    12601 ttaccttaaa tgattccatt tactttttat ctcatttttt cctactagtt agtatcaaat
    12661 actctcctat tttctcaata aactattttt ggatacatgg tatctgaaac agctaaaacc
    12721 ttgtcattac agcaaaaaat cacgttat
//
LOCUS       DANJMA010000064        12158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000064 DANJMA010000000
VERSION     DANJMA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(75..632)
                     /gene="frr"
                     /locus_tag="PWU31_002279"
     CDS             complement(75..632)
                     /gene="frr"
                     /locus_tag="PWU31_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL1084770.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(635..1357)
                     /locus_tag="PWU31_002280"
     CDS             complement(635..1357)
                     /locus_tag="PWU31_002280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL1084771.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1493..2374)
                     /locus_tag="PWU31_002281"
     CDS             complement(1493..2374)
                     /locus_tag="PWU31_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL1084772.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2473..3255)
                     /gene="rpsB"
                     /locus_tag="PWU31_002282"
     CDS             complement(2473..3255)
                     /gene="rpsB"
                     /locus_tag="PWU31_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL1084773.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3614..4093
                     /locus_tag="PWU31_002283"
     CDS             3614..4093
                     /locus_tag="PWU31_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL1084774.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4310..5401
                     /gene="phnW"
                     /locus_tag="PWU31_002284"
                     /pseudo
     CDS             4310..5401
                     /gene="phnW"
                     /locus_tag="PWU31_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5394..6179
                     /locus_tag="PWU31_002285"
     CDS             5394..6179
                     /locus_tag="PWU31_002285"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL1084775.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6525..8216)
                     /locus_tag="PWU31_002286"
     CDS             complement(6525..8216)
                     /locus_tag="PWU31_002286"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL1084776.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8638..9585)
                     /gene="arcC"
                     /locus_tag="PWU31_002287"
     CDS             complement(8638..9585)
                     /gene="arcC"
                     /locus_tag="PWU31_002287"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL1084777.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9700..10719)
                     /gene="argF"
                     /locus_tag="PWU31_002288"
     CDS             complement(9700..10719)
                     /gene="argF"
                     /locus_tag="PWU31_002288"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL1084778.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10810..12039)
                     /gene="arcA"
                     /locus_tag="PWU31_002289"
     CDS             complement(10810..12039)
                     /gene="arcA"
                     /locus_tag="PWU31_002289"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL1084779.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3338 a         2532 c         2188 g         4100 t
ORIGIN      
        1 aatttttaaa taaaagaaat ataaataaaa acagaggaag cggaatgatc cgcccctctg
       61 tttttatgat tttattaaac ttccaataat tctttttctt tatcagcagc gatcttatct
      121 acttctttaa tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca
      181 tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc atcacgacgg
      241 atgttacgaa tcgcgatttt tgcattttct gcttcttttt tcacatcttt agctaattct
      301 tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc atcattggtt
      361 ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag aatgctcttg
      421 tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc catttggttc
      481 aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct agcgttcgca
      541 cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt tttcattttt
      601 tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca gttgttccga
      661 tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg aagacaagca
      721 gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact tgtaatcctt
      781 tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca tctactttcg
      841 gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca tctgcgccga
      901 tttctgccgc tcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc
      961 ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg cgaatatatg
     1021 gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt
     1081 ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc atgtaatcag
     1141 cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata tttccgccac
     1201 ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct tggatgattt
     1261 ctttgattac tggtggtttg attccaaatc ctgcatcccc agctaaagct tctccactca
     1321 attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca atattttcta
     1381 ttgccatttt accatactta gatgatttcg acactctaag actggttttt cttgttttgc
     1441 ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag aattattttt
     1501 tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata ccttcgccaa
     1561 cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat ttttcaacag
     1621 tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct ttgaatttct
     1681 tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg ttcaatgctt
     1741 gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt gattcgttta
     1801 cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact gtttcgtcag
     1861 ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag tatccaccaa
     1921 atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt ttttctccga
     1981 taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt ttgattttca
     2041 ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt tcttttacta
     2101 aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact tcaacgattg
     2161 ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg cggtcatttt
     2221 tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt tccatgtcgc
     2281 cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc atttcgcgta
     2341 gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct
     2401 ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt gaaacagctc
     2461 catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta cgatttcttc
     2521 gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat cttcaccttg
     2581 gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg cacgaattgc
     2641 atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat
     2701 agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg
     2761 gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc ctaagaattt
     2821 ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta gaacttcaaa
     2881 agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt tttggatagt
     2941 atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt gtcctgcacg
     3001 agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga ataatgctac
     3061 gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta atttaactgt
     3121 tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt tcttcatttt
     3181 tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta attgtttcat
     3241 agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct tcttatcttc
     3301 taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat gtggatttgc
     3361 tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc aagagcaaat
     3421 atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt caagaaaaac
     3481 agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt aggtataaaa
     3541 gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta
     3601 aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga
     3661 attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc
     3721 cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga
     3781 aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga
     3841 ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca
     3901 ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga
     3961 tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat
     4021 ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt
     4081 atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa
     4141 tattttcctt attaaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa
     4201 ttttttcaac accttgattg aatcacaata atcatcattt atattggatg ttaacaagag
     4261 cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt
     4321 attaactcct ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca
     4381 ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga
     4441 tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta gcggctcctt
     4501 tggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc
     4561 aaacggcgca tatggacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac
     4621 cgtttatgag gtatcgtaac atcaaatcct ccaagctcat gtgattacac agctgattga
     4681 agagaatccc tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt
     4741 gaataatttg gaagagattg ctgctgtcac acaagaaaaa gatcttttgt ttatagtgga
     4801 cgcgatgtca agctttggtg ggataccgat tcgtgtagcc gaactaggga ttgatttttt
     4861 gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa
     4921 taaagcggaa ctagaaaagc gaaaaaatgt gagtcgaagt ttgtcacttg atctatatga
     4981 ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac
     5041 ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg
     5101 atattcacgt tatctcgcca ataatcatgt actacgacaa aaaatgacag agatggggtt
     5161 ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga
     5221 ctggcgattt gattttaatc atttttatca gtatttaaag caaaacggat ttgtgatcta
     5281 tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc
     5341 ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta
     5401 aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc
     5461 acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgcg
     5521 aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca
     5581 aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt
     5641 atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg
     5701 acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgactg
     5761 gatttactgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa gggtattcac
     5821 cagacttcat ggcaacttct gaagacgtcg aagggtatgg tcgaccgttt ccttatatga
     5881 tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag
     5941 atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg
     6001 aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa
     6061 aagatcgttt aaaacagtct gttgctctta gattccaaca agctggcgct gatatttgtg
     6121 taaacaattt aaaagagtta acagaataca ttttgtacgc tgacaagaat ccagaatgac
     6181 ttttatacaa aatcagaggt cgtgaaaaag ctgtactgca tcaagtaata agaacagcca
     6241 aacaaaaata gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt
     6301 ttgaacaccg tttattcgaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc
     6361 ttatttacaa acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc
     6421 tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt
     6481 ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt
     6541 gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga
     6601 gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg
     6661 ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc
     6721 ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta
     6781 tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg
     6841 ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca
     6901 acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg
     6961 actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc
     7021 tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc taataagacg
     7081 atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata
     7141 tggcgcatat cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta
     7201 aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt
     7261 ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc
     7321 ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc
     7381 tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag
     7441 aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat
     7501 tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttc atcgccttct
     7561 tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact
     7621 tcatggaatt ggacatataa acgaagcaat tcattgattg gttctgcttt aacagcttct
     7681 tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct
     7741 aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta
     7801 ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac
     7861 atgtcgattg gtactgttcc ttgattcccg atattgctat ccccataatg ttctttttct
     7921 ttcactactg tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag
     7981 taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct
     8041 gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca
     8101 acatctccgt gttcagctga ttttggattt tctagtaatt gttcgacttg ttccagtgtt
     8161 aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt
     8221 attcctccta aagtgctccc ttgattgtta aacaaaaaaa atctctagcg acggcatact
     8281 tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag
     8341 gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc
     8401 gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga
     8461 tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag
     8521 aaaaaaaaac gaaaggctag tcgtttctga taaaaaaaca atccagatgg cctaaaggag
     8581 aagaaatacc acctggatag ctataaatca tttcgtaaat acgaaagact taacaaatta
     8641 atcagctacg ataatcgttc cttcttcaga agctaaaaga ttctcgatat tttctaaaga
     8701 agtgatgatt gccttagcgt ttggttttgc ttcaacaaat gcaatagctg cctctacttt
     8761 cggaagcatg cttcctggag caaattgctt ttcatcaatg tattgtttca tttctgaaac
     8821 agtaacagtt tctaatttct tttgatctgg tttttggtaa ttgacataca cattgtctac
     8881 tcctgtcaat acgatcagca aatccgcatc gatgatttcg gctaattttt ctgaagcaaa
     8941 atctttatcg attacggctt ctcgtccttc taatccagta gctgtttcta caacaggaat
     9001 cccaccgcca ccaacagaga ctgtgataac tccttgatcg accagcgttt cgatgacacg
     9061 tgcttcttta atcgagatgg gtttagggga agcgactact tttctccatc cacgtccagc
     9121 atcttctttg aatgtcacag tggagtcagc attcatttgt tctttggctt cttcttccgt
     9181 gtagaatgga ccgactggtt tacttggatt tttgaatgaa ggatcatttt catccacaat
     9241 aacttgagtg actaaagaaa ccacatcttt atcgatccct ttttccttca gtacttcgcc
     9301 catggcattt tgtaaccagt aaccgataga tccttctgtc attgcaacac atgtatctaa
     9361 aggcattgcc ggtgttttct cagaatcagc agcttgctgc tgaatcaata ggttaccgac
     9421 ttgcggacca tttccgtggg agatgatcaa ttcatctcct tgttcgataa atttcactaa
     9481 atactttgct gtttccatta acgcttcttg ttgggctttt gcacttgcat ccgtagacaa
     9541 gattgcatta ccccctaaag cgacaactac ttttcgtttg gccatttcat tcgtcctttc
     9601 ttcagttcta ccgaattttc tagataacaa aacgagccag cgttttgtga ttttccctca
     9661 atgctgactc gttttttcgt gagatgaatg tggttatgct taaacgcgag ggataaacaa
     9721 gttacctaat gttgctgcca tgatcgcttt gattgagtgc atacgatttt ctgcttggtc
     9781 aaattgacga gcatatttgc tgcggaagac ttcatctgtt acttccattt cagtgatgcc
     9841 aaagcgttct ttcatttgtt caccgtaaac agtgtttgta tcatggaaag ctggcaagca
     9901 gtgcaagaag atcaaacgat ctgtgttgtg tgttttttct accatttcca tattgatttg
     9961 gtaaggtttc aacaatttga tacgttcttc gaatttatct tcttcaccca ttgataccca
    10021 tacatcagaa taaaggacgt ctgctccgtc tacaccttta tcaacatcgt cggtaatcat
    10081 caattgtgca ccggattttt cagcaaagcc ttctgccatt ttgacgattt cttcttcagg
    10141 ttgtaattct tttggtgcaa cgatacgcat attcgctcct aaaattgcac ctgtgactaa
    10201 taaagagtta gccatattgt ttcgaccatc tccgcagtaa gcaacagtga taccttctac
    10261 tgttccaaag ttttcttgga tagttaaaaa gtcagcgatc atttgtgttg gatgccattc
    10321 atcagtcaag ccattccaaa ctggaacacc agagaattct gctagttcct ctaccatttt
    10381 ttgactgaat ccacggaatt cgattccatc aaacatacga cctagtactt tagctgtatc
    10441 ttctgtggat tcttttttac ctaattggat atcatttgca cctagatatt caggatgtgc
    10501 acctagatcg atcgctgcag tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa
    10561 taaaagcgca atattttttc cttctagata gtgatgtggg ataccacgtt ttttcaagtc
    10621 tttcaagtgt tctgaaaaat cgatcaaata ttgtaattct tcttttgtaa agtctttttc
    10681 tgctaataag cttcttcctt ggaatactga ttctttcata ttaaacattc tcctttagat
    10741 tgtttttatt taagattaaa gaggaaatcc cgacggcagc tgacagtaag accgccgtca
    10801 ggaaacaaat tacttcaagt cttcacggac aagtggttgg ctcatgcagc gtggaccacc
    10861 acggcctcgt gacaattcac ttgaattgat ttctaacact ttgatgccat agctacgaag
    10921 caattcgttc gatacataat ttcggttata agtgactaca acacctggag cgattgccaa
    10981 tgtgtttgat ccatcattcc attgttctcg aggtgcaacg atctcatctc cattaccagt
    11041 tgggatcagt acaagatcat ctaatccaag agcagctttc atcgtttcgt gtaagttatc
    11101 tgaatgagta atcttgatat catcaccatc tggttcgatt gtaaagacat ccatcttacc
    11161 gtctttactt tgaattgctg gatggatagt gaatttatcg tgatctacca ttgtaaatac
    11221 ggtatccaaa tgcatcattg cacggttgtt tggaattttg attgctaaca ctttttcaaa
    11281 tcctgaattt tttgcgaata ggtggcgtgc taatttttct aaagctttcg cattagttcg
    11341 ttgagaaatc ccaacagcta ctactttatc gcttaagatc aattcgtctc cgccttcgat
    11401 atgatctgga ttttcacgat ccaaccatac ttcgattcct ttatttgcaa atcttggatg
    11461 atgtttcaaa atatattctg taaacatcga ttcacgacga cgtgcttcaa aagtcatctt
    11521 attgactgtc atcccatttc ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg
    11581 cattgggtcc atgtaaaatg gatagtcatc gtctgctgac agatcgtata gactgctaga
    11641 acgtacttca atatcttttg tacgaacccc agccatgatt ttatctacca tttcttgcgt
    11701 ttccattgat agaaggaact catgaagacc ctctcttaca gcattcgatg caatatgaga
    11761 ttcatccaac attttattca agaattgttc tttgacattg cctgcgtcaa tcgcttctgc
    11821 cgctaatttt tccaagtaaa gagtctcgac gccttcattt tgcaatgtct ttgcaaagtt
    11881 gtcatgttct tcttgagcaa tcggaagata tggaatatca tcgaacaaca gacgatccat
    11941 aatatctggt gtcaaatttt ccacttcttg tccaggcctt ttcagtaaaa ctgtctttaa
    12001 tttccctatt tcagagaaaa cgtgaatagg tttatccatg ttgtgttgcc tccttctttt
    12061 tgattacatg tataggatat cctccttttg taatcacttt cataattttg tctcattcaa
    12121 aatgagacat tcctcacatt aatattcgat aattaaat
//
LOCUS       DANJMA010000065        12136 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000065 DANJMA010000000
VERSION     DANJMA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..610
                     /locus_tag="PWU31_002290"
     CDS             <1..610
                     /locus_tag="PWU31_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084780.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="PWU31_002291"
     CDS             723..1001
                     /locus_tag="PWU31_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1084781.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="PWU31_002292"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="PWU31_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL1084782.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="PWU31_002293"
     CDS             1458..2213
                     /locus_tag="PWU31_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1084783.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="PWU31_002294"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="PWU31_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL1084784.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="PWU31_002295"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="PWU31_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL1084785.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="PWU31_002296"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="PWU31_002296"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL1084786.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="PWU31_002297"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="PWU31_002297"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL1084787.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="PWU31_002298"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="PWU31_002298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL1084788.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="PWU31_002299"
     CDS             6950..7492
                     /locus_tag="PWU31_002299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL1084789.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /locus_tag="PWU31_002300"
     CDS             7515..9071
                     /locus_tag="PWU31_002300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL1084790.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="PWU31_002301"
     CDS             9085..9987
                     /locus_tag="PWU31_002301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL1084791.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="PWU31_002302"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="PWU31_002302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL1084792.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="PWU31_002303"
     CDS             11430..11852
                     /locus_tag="PWU31_002303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL1084793.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DANJMA010000066        12108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000066 DANJMA010000000
VERSION     DANJMA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            212..1690
                     /locus_tag="PWU31_002304"
     CDS             212..1690
                     /locus_tag="PWU31_002304"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL1084794.1"
                     /translation="MKLKENWSRTDRYRPYKEWSEEHLHSLEKKINSSHWRLGYHIQP
                     VTGLLNDPNGFSYFDGKWQLFYQAYPMGSVHGLKSWYHLSSVNLIDWKNDGLKLIPDN
                     SFDSHGVYSGSALPIDNQLFLAYTGNVRDSNWGRHSFQLGAWMDKNGNITKIPTPLIS
                     SPPPGYTQEIRDPQLIAYDDGYLLIVGAQTEQKQGRILTYYSKDLQNWQFLGTLGFTE
                     EEMGFMIECPNLLFVNGKALLVFCPQGLSHEIHSYSNIYPNTYVIGQTFDFEKNELSD
                     PTHIKNIDEGFDIYATHCFSDENGRTLAISWIGLPEISYPTDEEGWAHCLSIVKELTI
                     EKEQLYQRPVKETAQLRKGKPYHFSGLETTIDAEINQYELQLEFDYSQKGTILLFADE
                     QQKHGLALSFDMIHGKMSMDRSHAGKSFAENFGTVREFSIEKKPLKLQIFVDRSVVEV
                     FVNDGQQTATARVFPTDDQTGLYIKSDREFKGKLWHLRNMNK"
     gene            1703..2686
                     /locus_tag="PWU31_002305"
     CDS             1703..2686
                     /locus_tag="PWU31_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084795.1"
                     /translation="MAVKLTDVAKKAGVSPTTVSRVINNYGSLSQKTIDKVNQAMKEL
                     NYQPNSLARSLQGKNTQLIGLIFPTVANPFFGELVEQLESKLFDLGYRSILCDSANNK
                     EKERNYLNMLAANKVDGIIAGAHNLGIQEYENIELPIVSFDRYLADGTPIVSSDNFRG
                     GYLATENLYLKGARKIAILTGSEESNSPTNDRMNGYLKFMDEQQLEPQIFQFHSTARS
                     TALKNLEIKRILQNEEIDSIFCTDDLTAVLAYNLCQELDIRIPEQLKIIGYDGTKFIQ
                     NYFPHLSTIAQPITDIADILVDLLLQRIQDAEKPLESHYVLPVKLIQGKTT"
     gene            2708..3589
                     /locus_tag="PWU31_002306"
     CDS             2708..3589
                     /locus_tag="PWU31_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1084796.1"
                     /translation="MLYGAIEAGGTKFVCAVGTKDLEVIERIQIPTTTPIETMNNVID
                     FFKKHQEQLAAIGIGSFGPIDIHPNSATYGYITSTPKKAWQNFDFIGYLNKNLKELPI
                     VFTTDVNAAAYGEYVAGNGKGRSTIVYYTIGTGIGGGALQNGTFVEGFSHPEMGHMLV
                     RRHPVDTFKGVCPYHSDCLEGMASGPAIEQRTGMKGQELSVADPSWELEAYYIAQCVY
                     NTTLLFSPDVIIFGGGVMKQAHLRERVQEALVGLMNHYVDYPAFENYVLPPKLGDDAG
                     IIGCLALALEKSEKDIF"
     gene            complement(4341..4904)
                     /locus_tag="PWU31_002307"
     CDS             complement(4341..4904)
                     /locus_tag="PWU31_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1084797.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(4919..5962)
                     /locus_tag="PWU31_002308"
     CDS             complement(4919..5962)
                     /locus_tag="PWU31_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084798.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDKKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMQTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(6095..6499)
                     /locus_tag="PWU31_002309"
     CDS             complement(6095..6499)
                     /locus_tag="PWU31_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1084799.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(6667..7302)
                     /locus_tag="PWU31_002310"
     CDS             complement(6667..7302)
                     /locus_tag="PWU31_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL1084800.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLGSMIFGVLTRLE"
     gene            complement(7568..8620)
                     /locus_tag="PWU31_002311"
                     /pseudo
     CDS             complement(7568..8620)
                     /locus_tag="PWU31_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8633..9571)
                     /locus_tag="PWU31_002312"
     CDS             complement(8633..9571)
                     /locus_tag="PWU31_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1084801.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(10022..10672)
                     /locus_tag="PWU31_002313"
     CDS             complement(10022..10672)
                     /locus_tag="PWU31_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084802.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(10772..11281)
                     /locus_tag="PWU31_002314"
                     /pseudo
     CDS             complement(10772..11281)
                     /locus_tag="PWU31_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
     gene            complement(11644..12027)
                     /locus_tag="PWU31_002315"
     CDS             complement(11644..12027)
                     /locus_tag="PWU31_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1084803.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNKVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         3817 a         2425 c         2015 g         3851 t
ORIGIN      
        1 caaaattatt gaagcagtaa ctataaattt ttcttaagca caatatgcca tgatttaaaa
       61 caattgtcaa tcgattatca aattttaaat aaaataattt tttatatata aaaaaatagc
      121 ttttataagc catttaattt tgatattcgt ttgacatttt accagaaaaa atatattata
      181 attccaaaaa tacgtttaca aggagtttgt catgaaactt aaagaaaatt ggtcacgaac
      241 ggatcgttat cgtccatata aagaatggtc tgaagaacat ttgcattcct tagagaaaaa
      301 aatcaattct tcccattggc gcttaggcta ccatatacaa ccagttactg gtttattgaa
      361 cgatccgaat ggtttttcct atttcgacgg gaaatggcag ctattttatc aagcatatcc
      421 gatggggtct gttcatggtc tgaaatcttg gtaccatctc tcctcagtta atctgataga
      481 ttggaaaaac gatggcctca aactgattcc ggataattca tttgacagtc acggtgtgta
      541 ctcaggttca gctttgccga ttgataatca actgttcctt gcatataccg gaaatgtgcg
      601 tgattcgaat tggggacgtc attccttcca gttaggggca tggatggaca aaaatggtaa
      661 tatcactaag ataccgactc ctttgatcag ctccccacct ccgggatata cacaagaaat
      721 ccgtgatcct cagctgattg cttatgatga cggttacttg ctcattgtcg gagcacaaac
      781 tgaacaaaaa caaggaagaa tcctaactta ttacagtaaa gatcttcaaa attggcagtt
      841 tttaggaact ttgggtttta cagaggaaga aatgggtttt atgatcgagt gtccaaatct
      901 attatttgtg aacggcaaag cactactggt cttctgccca caaggtttat ctcacgagat
      961 ccactcatac agtaatattt acccgaatac ttacgtgatc ggtcaaacat ttgattttga
     1021 aaaaaatgag ctttccgacc ctactcacat aaaaaatata gatgaaggat ttgacattta
     1081 cgcgacgcat tgtttcagtg atgaaaatgg acgaaccttg gcaatcagtt ggattgggtt
     1141 gcctgaaatc agctatccaa ctgacgaaga aggatgggca cactgcttga gcatcgtaaa
     1201 agaacttact attgaaaaag agcagctcta tcaacgaccg gtgaaagaaa cagcgcagtt
     1261 gcgaaaagga aaaccttatc actttagcgg tttggaaacc actattgacg cggaaataaa
     1321 tcaatatgaa ctccagttag aattcgatta ctcacagaaa ggtactattt tattgtttgc
     1381 agatgaacaa caaaaacacg gtttagcact atcctttgat atgatacatg gtaaaatgag
     1441 tatggatcgc agccatgccg gcaaatcttt cgctgaaaat tttggaactg tacgtgaatt
     1501 ctcaattgaa aagaaaccgc tgaaattaca aatatttgtt gatcgttccg ttgtagaggt
     1561 gtttgtaaat gatggacagc aaacagcgac tgcccgtgtc tttccgacag atgatcaaac
     1621 gggcttgtat ataaaaagtg acagggaatt taaagggaaa ttatggcatc tgcggaatat
     1681 gaacaaataa aggatgaaga aaatggctgt taaattgaca gatgtggcaa aaaaagcagg
     1741 agtctctcct actactgtct cgagagtgat aaataattat ggttctttaa gtcaaaaaac
     1801 aatcgataaa gtgaatcaag caatgaaaga actcaattat cagccgaatt cgttagcccg
     1861 ttcacttcaa ggaaaaaaca cccaattgat cggcttgatt ttcccaacag tcgccaatcc
     1921 gttttttggc gaattagtcg aacagttgga aagtaaatta tttgatttag gctatcgttc
     1981 tatcttatgt gacagtgcta ataataaaga aaaagaacga aattatctca acatgttagc
     2041 agcaaataaa gtggacggca tcatcgccgg cgctcataat ctcgggatac aggagtacga
     2101 aaatattgag ttgccaatcg tctcttttga ccgctatctg gcagacggaa caccaatcgt
     2161 cagcagtgat aatttccgag gcggttatct cgcgacagaa aatctctatc taaaaggagc
     2221 tcgtaaaatc gctattttga cgggctcgga agaatccaac tcccccacaa atgaccgaat
     2281 gaacggctat ttgaaattta tggatgagca gcagctagaa ccacagattt ttcaattcca
     2341 ttcaactgct cgttccactg ctttaaaaaa tttagaaatc aaacggattt tgcaaaatga
     2401 agagattgac agtatttttt gcacagatga tcttacagct gtattggcat acaatctgtg
     2461 tcaagagctc gatatccgca tccctgagca attgaaaatc attggatatg acggaacaaa
     2521 atttatccag aattattttc cgcatctgtc tactatcgct caaccaataa cggatattgc
     2581 cgacatcctt gtagacttgc tactgcaaag aatccaagat gctgaaaaac cactggaatc
     2641 acattacgtt ttaccagtaa agctgattca aggtaaaacg acttaactgc aaaggagcaa
     2701 atgttttatg ttatatggag caattgaagc tggcggaaca aaatttgtct gcgcagtagg
     2761 tacgaaagat ttagaagtaa ttgaaagaat acaaatccct acaaccacgc cgatagaaac
     2821 gatgaataac gttattgatt tcttcaaaaa acaccaagaa caactggcag cgattggaat
     2881 aggttctttt ggaccgattg atattcatcc caattcagca acatacggat atatcacctc
     2941 tacgccaaaa aaagcttggc agaattttga ttttatcggt tatttaaata aaaatttaaa
     3001 agaactaccg attgtcttta ccaccgatgt caatgcagct gcttacggag aatatgtcgc
     3061 ggggaatgga aaagggcgtt ccactattgt ttactacacg attggaacag gtattggtgg
     3121 cggtgccttg caaaatggca cttttgtaga aggtttcagc caccctgaaa tgggacatat
     3181 gcttgtgcgc cgacaccctg tggatacctt taaaggtgtt tgtccttatc atagcgattg
     3241 cctggaagga atggcttctg gacctgcaat cgaacaaaga acaggcatga aaggtcaaga
     3301 gttgtctgtc gctgatcctt cttgggaatt agaagcttat tacatcgctc aatgtgtcta
     3361 taacacaact cttctatttt cacctgatgt catcatcttt ggcggaggcg taatgaaaca
     3421 agcacatctg agagaacgag tccaagaagc tttggttggt ttgatgaatc actatgtaga
     3481 ctacccagca ttcgaaaact atgtattacc tccaaaatta ggagatgatg ctggaatcat
     3541 tgggtgttta gctttagcat tagaaaaatc agaaaaagat atcttctaat aaaataagca
     3601 cgttgtcaca acaatctcat caataagcag gcgaagaatc cacttgatgc gatttcttcg
     3661 tctttttatt tactattaac ttatgttctc caatcggcct ttcaacccga tatcctcgcc
     3721 tatttttctt gcccttaact ctctgtttcc tgtacttaac agagtagagg tgtcgtggaa
     3781 ttcttttgtt aacttcaaat tatgatcttc tacaagttcc tacaatctaa tcttggttgt
     3841 ttttttattc aacttgcaac gctagcaagt tccagttgat tctcttttcc aattaagttt
     3901 tgtgatattt aaacttcgcc ttgacagctt ttctaaatat agaaaagaac atttccttat
     3961 gtctattatg caacagctca atttatttaa tgtatgttaa tgattttgtg gaatgtctcc
     4021 ttagacatgc tatttctact atttaatgat caatcacttg acctactagt aagtagagag
     4081 catgcccgaa cacattacaa aaatatcatc acttatgata cggagctgcg tatcatgagt
     4141 gaatgatatt aggaaaactt cataaaataa atcttttata catttccact tctagatggt
     4201 cattcatgtt agaagaatca aactattttt ttactgtcgt aataatatta aaacactaga
     4261 acaaatcaat taaattccat aaaatataaa taatagacct agcatcatct ggtataaaat
     4321 ggatgctagg tccgttatat ttactcaaaa ttgattctac gaatagatct tcttatcatt
     4381 tctattttag tagtcatgcg ttgttgttct tctaaaggtg gtaatggtat aagtagttta
     4441 gataaatttg taccatttac atttggttgc ccagttccgt aagaaatttt ttctaataac
     4501 ttccaatata atggcgagtc taagaagcag tctacgtatt ctgataataa tttctcaact
     4561 aacctaattc gaatcaagta agaagcaaaa acagattcct cacttatttc cttaaccaag
     4621 aaactttttc ccatagttcc tcctgtcctt gctatcaaaa tgtcattttc ttccaatctt
     4681 aaatcaacta gtttagaatt actaatatca cagtaaggca cactggacca atttacacgt
     4741 ccctcttgta tatctgtgat tctcaaaaac ttaacattgc cttgtttttt cgccgaatct
     4801 gtatagccat attgaatctt agtagaaata cttcctaaat aaacccatag tcgaacatcg
     4861 ctagatgatt tttcttgtcg ttctttatga acaacagtga tcatcttgag ttgtccgact
     4921 attttttagt tgcagcaatc tttttgggac gcttatttgc atgaagcaac gttgctaagt
     4981 ctagtaataa aacattggct aacacaggat tggctttctc gcgatacgct gcggcgccac
     5041 tttcaaacaa tactgctaga cgatcttcta caacaacgat ttgttccaaa tacggtggaa
     5101 atttgattcg ttttaggatc cggctaggag agtcccgctc ggacactaag agttccgagg
     5161 tataaggacc ataagaacga ctggctaaga gataatggcc ataaaaagcc acaccttgaa
     5221 tcttatcaga tgtttttctg ctcattgtca cggttggttg gattttgtct ttgcggcgaa
     5281 ttgctgtttg catttcagct atcgtcggca atagatagaa agtcaactgt ccttcacctt
     5341 ttagtgtgaa gttgccagat accagttgat cattgttttg agtcaaatac gatgcttgtg
     5401 gaatctctga gagatcagtt gtcagccaat aagcgattgg ctgtcccgtc tcttcagatg
     5461 tatctgctaa gatatcgatc aaacgtaaag cggctactcg tccagtagca gatcctgtga
     5521 ttgtcaacca taaaagttcc cgttttttat catatactaa gccccctaca tgtggccgtt
     5581 taggtaaaat cagcagtttg atcggctgtc ctgtttctct atccagtaca tgaataatag
     5641 aatgatgctg atggtcatag cagtaggcag aaataaacac aaaatcttga gaaaaagcca
     5701 acccttgcgg cgtcatccat tgacaaacct cattttcgcc ggtttccggc accaccattc
     5761 ttgtttctgt taatccagga atgttatatt gttttgcttc agccttttcc caagctgctt
     5821 gaacaatagg ataccgtttt aacttttttt caatatcctg atgtatccgt tgctgcttcc
     5881 aatgcttaaa ctttaggaca tagtaaacaa ggaggccaat gaccagcaac actaaaatca
     5941 ttattgctac ccgctgtgtc atttttttgc ctccttgttc tattcgatca atcgtacata
     6001 taccgctatt aattaccgta actacatgtt tcttttacta gcgtttcttt ataggatctt
     6061 tcataatctc atttatagta acattaagaa tcccttattc tactatagtc acttccacat
     6121 caatattgcc tcttgttgct ttagagtatg ggcaaaatgc gtgggcttct tctgcgattt
     6181 tctctgcttg gtccttctca agtcctggca aagaaacttc taaaacaacc ccgatttggt
     6241 agcccccatc ttctaattga aataatgata cccgtgcttt gacttcagaa tcagactcaa
     6301 gattattttc ttccagcatg tgttgaaccg cgccgttgaa gcaagatgcg tatccagccg
     6361 caaaaagttg ttccggattt gtcccttcac tatggattcc tgggcggtcg atcttcatgt
     6421 gcatacttcc gtttggtgcc tcaacctcgc catcacggcc tccacgattg atcattgctg
     6481 tttcgtacat ttttttcatt gagatcatcc tttctttcgt cgttctatat taagtgtaac
     6541 gagtattttt taatctgaca aaggtaatgc ttgcattcgg cgacatcagt tagacaacat
     6601 cgatgatcag tgatagaagc agtttatcta tacaaaaaaa gaagggttct atgatatttg
     6661 gtgttgttac tcaaggcgag taaggacacc aaatatcata gaacccaaat agttaaagaa
     6721 aaaacttaat cgttgaaaga agcccaattg cttaaagaag gcaaactgac taatcgacgg
     6781 aatccgtgct aatccgtaaa gacctgcaat tagtaaacta ataactgtta aaacaagata
     6841 aaaatggcta agcgtacctt gaccactttg acgataaagc agaacaagta acaatgggaa
     6901 aagtagaaag cctgcatagc ctagcccaga acctatatta tggatccaag tcgatagatt
     6961 ccaagagctt tcattcgtat tgatactgaa taacccagta aaaatacaat cacctatccc
     7021 atataaacca actgttgcaa cagcaagtat tgcaagtgtt tttgatgttc caacaaatag
     7081 gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca gatacaactg accagaccaa
     7141 gaaagcacgt ctgactggac tatctacgtc accaaacaca ctaattacgg tagtcatttg
     7201 attcagtttg ggataaaaaa ggccaaggat atacggtgtc gataaatcac taatcacacc
     7261 taataataaa aaataaaaac catatttttg caaaaaaatc attttcttcc cctacttttt
     7321 agtgattatt cgatacatcc aacaatcaaa ttttttcaca ttttaacaca tatttttatt
     7381 ctagttgcct tttgaacaat caataacata aaggtgagtt tcttctacat tttaaaaaat
     7441 cgaatggcac tgaagagttc agttgtcagc atcaatctcc tactcaaaaa ggcaaaaaaa
     7501 cgtaagtcag atgttgttga catctcactt acgcatccat tgattgaaaa tttcttagta
     7561 aaatctacta gcctaaaaat ttgaagactt cttctcgatc atacagttta aatactttct
     7621 cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat gactaaccaa caaagaacaa
     7681 caagaaagcc atttagccct gcatctaaag aagccaacgg accaacaagt ccgaccacgc
     7741 cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag agcgacagga atagctgtaa
     7801 tcactgcact aactaaacaa ggaataagaa tcacaggttt tctaaataga ttaggcatca
     7861 tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc tgacttatta accttccaag
     7921 agttcactac taaaacaaaa gtggttgcag ctacgcccat cgcagccgca ccagcagaaa
     7981 caccatctag ttggattgcc attccgattg ctactgttga aataggagag ataatcaaaa
     8041 aggcaaagga acaaccaatc aagatagaca ttaacagcgg ttataaggta gtgaagttat
     8101 taatcaatga tccaattgca gaagtaattg ctgttacaaa agggaataaa taaaaaccaa
     8161 gtaaacctgc acctacacct acaacaatcg gtgttaacac aatcgacact gacccaaaac
     8221 tatttccaat cagtaaaatc agcccgacag caatcgatgc tgtcagcatc gtattaataa
     8281 tatcgcctgt accagcacca acgtaaacac ccgtttcacc caaatcaggt gtgaatttga
     8341 ctacaccaga accaacaaaa gaagctccgg ctacaaccat cattctcatc ggatcaaatc
     8401 caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt tgctagttga aaaataactg
     8461 ctaactgtgc gatcgttacc gcaaagggat acgcactaaa atattttaaa atggcaccta
     8521 atactgcatt aggaatcaat ccaataatga ttcctgttgc tgtaccagcg aggactttat
     8581 tcagaaaaat tttcggtgat attttttgtg aagtttccat tcaatctctc ctttatttta
     8641 cgttcaaact atcttctttt gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg
     8701 caacaccata tttcttccct tttctagaaa ttgcgccatt gatataatcg atttctgtta
     8761 atcgatggtt cttaatcaag tcttgataca ttgaaggata atgtaaccca attgtttccg
     8821 ggtcaaaaca agattcacaa tgtgcaatga cttctgggac atctagttca atcttctcta
     8881 ctgctgctac tttggcaaac tcattgacaa tcgtcgccac cattttatga gcagtcgatg
     8941 tttttcctag ctcagccata ttaacgtcta agattgtgca tagtccgttc attgttccgt
     9001 tcacacatgc tttgcgataa atagaatagt gaatattgtc agaaaaatga gcatttaatc
     9061 ctgattcaga caatttgtct gctaattttt ttgcagctgc ttcttttcca tcacctaaat
     9121 tttgtaattc tactgaacca cttccaaata atttgacctg accaggacct tcaagacctg
     9181 ccgtccacat ggtatttcca atatagatat tttccattgg tacaaatttc tcaataatat
     9241 cttcatgccc gataccattt aatagacata agacctctgt atcttttttg attaatgatt
     9301 ggatatcctg cagcattttt tccagctgca tcgctttggt aaatagaata atcagatcaa
     9361 cttgatcttc tttttctact tcggattgaa gaacgattgg tagttttgct tctacctctt
     9421 taccattaaa atttgcttgt aatccatgtt ctttgatttg ttggacatgt tctgcccagc
     9481 catcgattaa taatacttcg ttcccacttt ggtgaagcat taacccaaag cgacttccca
     9541 ttgcgccagc gcctgcaatt gctattttca ttgattcttc ctcctttttc tttctattat
     9601 acttatttga gaatcatttc acaaccttgt tttgttattc ttctcttatt tatcatgttt
     9661 atatccacca ttcaagttat tttatcagca aatcaataaa aactggaata gaatcccaat
     9721 ttcatcgtaa gagaaactag actagcaata agaaaataga aacattcata gaaaaaacct
     9781 cactcctctg attataaaag aggggacatt aacctcgtgt atactaaaac cttccattca
     9841 ttcgatggat gattttcttt ttgtgaaaaa atattcattg tagtacgatg ttgcgcaaca
     9901 cagacggatg gtattgctta ttatttatta aaatcgaaca atacacttat ttaaaaactt
     9961 ctttcccaga ttttcttcca tttttacagt tataactagt attatcatga aaaatagcct
    10021 tttattgtct atgggaataa taatttaaaa ttggtacttt tttattaatt agttcatcgc
    10081 ccaataatct ctccagtttc ttagcatctc ctgactcaac cggaattatc caagcccagt
    10141 ccaaataatt ttttaaaatc tcatcatatt ttgcaaatat ttcattgcca tattccgtcc
    10201 ttaaaattat attttcagtg ccagacaagt gatttgtatt gagttgataa aaaagatctc
    10261 catctaaaaa aatatcatta gaagaagatt tccccacagt aatgaccatt ccatcagctc
    10321 tatgtgttac atacaaataa ttgtatgacc tcacttcacc taatgaatca attatttctt
    10381 gccctttagc tctaatatat tgatctctat tacctatctc actcttattt aacaaaagtt
    10441 ttttcatgtc aaatttaatc ggctgtttta tttttgtaaa ttcgcttcta ctactaatcc
    10501 aatccgagat taatttttca acatgtccca gagtaaactc tgatttattc gctaaatact
    10561 ttttttgttg tattaaagac atctttcccc gaagctcaaa attgagccta catcgattct
    10621 ctatctcttc aaaaagcatc ttatcttcat cattcttata ttcatcattc ataaaaccaa
    10681 tccagctcct cgattaagat aatattttaa tctcatacaa aaactagctc tcatcaaaac
    10741 aaccattgtt ttgatttctc atgaaatttc tttatacttt tctccgatta taaattccta
    10801 accccttgag aaaattacgc atatatttat ctccgcaagg cttaaaattt cgttggcctt
    10861 cttttctcaa aaatgccccg atttcgcctt ttgatggata gatttctgcc tcagcaaaaa
    10921 cgtctaaaat atcatcagtg gtcatagaca aggcgatttt taattttttt agcaacacat
    10981 tgttgatata tttgatttcc ttgctttttt acggcgtgtc tactatcggc ttatcttttt
    11041 gcggaccacg tgccaagaga atcaggccat ttaggaatgc gtcaaaaact tggttgttga
    11101 tggttttttc atacacatta ttttctgcat tatctttgtt cttatcctgt actttggtca
    11161 gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc tagctcaaaa gctttaatca
    11221 aatccgtatc tttcaaatca atcgcataac gcaatcgcaa taacctatca ttgttattca
    11281 tttgttcctc cgaagtattt agtctttact ttcttacgta ctttttgtct tgctaagatt
    11341 tttaggatca atgataaaaa ccgcatcact tatgatacgg agctgggtat catgagtgaa
    11401 tgatatttat aaatcactgg atattgaagt atattctgtt ttctcaactt atttagtcag
    11461 attttttaat gaagattttt tgagtaaaat ttctataatt cctacttatt gaaaagtagt
    11521 ttgtagtgct gcttctacct ctctcatctg ttctacaaat tccaaataac ttgacgcagc
    11581 ttgcggaatg aaaaaatgat atgtgaatat ttctcctcct agcccgctta atatgcctat
    11641 tttttatacc catgatttgc aggaagggga tcggaagtag gttgcagaaa gccgcctttc
    11701 ataatctgtc ggaaaccgga catcaaaacg gattatttta cttgtcacat ttgtattttc
    11761 ttgtgaggaa gtgccaaata ccttatttac ttgagtgaca ttctcattgt cctgtctgat
    11821 attgcttcgt ataaaagtac cagatccacg tctcgtatat aacaaacctt catcaataag
    11881 gagttgtata gctttgcgga tagtaacacg gcttgtttta aactctctag ccagactttc
    11941 ttgctcaggt aatttagaac cttcctgata ttttccctca acgatcctct cttttagagc
    12001 caaggctatt tctttatatt ttggcatatt ttcccctcat ttcattgatc ctacaaaaaa
    12061 attagtatat atacaaattt ttgtaaagta tatatactaa taaataca
//
LOCUS       DANJMA010000067        11445 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000067 DANJMA010000000
VERSION     DANJMA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            185..1183
                     /locus_tag="PWU31_002316"
     CDS             185..1183
                     /locus_tag="PWU31_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1084804.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="PWU31_002317"
     CDS             1295..1804
                     /locus_tag="PWU31_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL1084805.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="PWU31_002318"
     CDS             complement(1834..2493)
                     /locus_tag="PWU31_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL1084806.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="PWU31_002319"
     CDS             complement(2495..3259)
                     /locus_tag="PWU31_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL1084807.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="PWU31_002320"
     CDS             complement(3267..3944)
                     /locus_tag="PWU31_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL1084808.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="PWU31_002321"
     CDS             complement(3981..5366)
                     /locus_tag="PWU31_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1084809.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="PWU31_002322"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWU31_002322"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="PWU31_002323"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="PWU31_002323"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL1084810.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="PWU31_002324"
     CDS             6305..7162
                     /locus_tag="PWU31_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084811.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="PWU31_002325"
     CDS             complement(7243..7578)
                     /locus_tag="PWU31_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084812.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="PWU31_002326"
     CDS             complement(7645..9015)
                     /locus_tag="PWU31_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL1084813.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="PWU31_002327"
     CDS             complement(9093..9629)
                     /locus_tag="PWU31_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1084814.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="PWU31_002328"
     CDS             9938..11419
                     /locus_tag="PWU31_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084815.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2300 c         1855 g         3773 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttatt
//
LOCUS       DANJMA010000068        11194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000068 DANJMA010000000
VERSION     DANJMA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            156..1415
                     /gene="lysA"
                     /locus_tag="PWU31_002329"
     CDS             156..1415
                     /gene="lysA"
                     /locus_tag="PWU31_002329"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL1084816.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1402..2202
                     /gene="dapF"
                     /locus_tag="PWU31_002330"
     CDS             1402..2202
                     /gene="dapF"
                     /locus_tag="PWU31_002330"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL1084817.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2205..3086
                     /locus_tag="PWU31_002331"
     CDS             2205..3086
                     /locus_tag="PWU31_002331"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL1084818.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3083..3799
                     /locus_tag="PWU31_002332"
     CDS             3083..3799
                     /locus_tag="PWU31_002332"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL1084819.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3805..4500
                     /gene="dapD"
                     /locus_tag="PWU31_002333"
     CDS             3805..4500
                     /gene="dapD"
                     /locus_tag="PWU31_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL1084820.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4516..5517
                     /locus_tag="PWU31_002334"
     CDS             4516..5517
                     /locus_tag="PWU31_002334"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL1084821.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5514..6875
                     /locus_tag="PWU31_002335"
     CDS             5514..6875
                     /locus_tag="PWU31_002335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL1084822.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6835..7986
                     /locus_tag="PWU31_002336"
     CDS             6835..7986
                     /locus_tag="PWU31_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL1084823.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEIIQSI"
     gene            7961..9130
                     /locus_tag="PWU31_002337"
     CDS             7961..9130
                     /locus_tag="PWU31_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1084824.1"
                     /translation="MMKLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9305..10684
                     /locus_tag="PWU31_002338"
     CDS             9305..10684
                     /locus_tag="PWU31_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1084825.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10965..11194
                     /locus_tag="PWU31_002339"
                     /pseudo
     CDS             10965..11194
                     /locus_tag="PWU31_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3730 a         1953 c         2498 g         3013 t
ORIGIN      
        1 tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact agacaattca
       61 gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg aaattccaat
      121 aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata attggcggag
      181 tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat gatgaagaaa
      241 agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt tttgatacaa
      301 aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta gttcgtgaat
      361 atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg caagctgatt
      421 ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa ctgatctatg
      481 cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt gaattattag
      541 cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag
      601 tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga
      661 gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc agctatctag
      721 agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact gcttggttag
      781 ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt tccttgaatc
      841 ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca gtcgaagagg
      901 cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa ctgaccattc
      961 gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca acactataca
     1021 cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat ggcgggatga
     1081 cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta gcagcaaaac
     1141 tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg
     1201 tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta gctgtttatt
     1261 ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca cgaccagcag
     1321 ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca ttttctgatc
     1381 tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg aaatgatttt
     1441 atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc tttatgcaca
     1501 aaagaaaaat atgatacaga cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc
     1561 tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct
     1621 cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga gacattagca
     1681 ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt agatatggga
     1741 gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag
     1801 gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg aacgattcat
     1861 accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc
     1921 tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa
     1981 caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca
     2041 ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc agatcaggta
     2101 gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat acaaatggct
     2161 ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata aaaggttcga
     2221 tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct ggtttggaaa
     2281 aattgatcca attccattta gatgaacaaa cagatggatt attagtttta ggtacaacag
     2341 gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg acagtaaaga
     2401 aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg aaagaaacga
     2461 ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc atcacccctt
     2521 actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt gcagaagcct
     2581 caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg attccaatcc
     2641 atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa gcctcaggag
     2701 atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc ctttacagtg
     2761 gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta ataagtgtat
     2821 gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag gacggaagat
     2881 ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc ttgttcagtg
     2941 agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct tccggacctc
     3001 tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct cagcttttgt
     3061 taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac agatgggaaa
     3121 tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac ggacggcacc
     3181 taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg atttttctca
     3241 tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc cgttagtcat
     3301 tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg ccaaacatgt
     3361 acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc aactaatcaa
     3421 gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac atcatagtcg
     3481 taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc aagaagtgca
     3541 aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt
     3601 acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg ctgctactga
     3661 tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg caggcgcagt
     3721 agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag aagatttatt
     3781 aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg
     3841 gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa
     3901 gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta tgagacgatt
     3961 gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga aactgctgca
     4021 agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat cgaaccaggt
     4081 gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat gggagcgatt
     4141 atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc tgtcttaggt
     4201 ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt agcaggtgtg
     4261 atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt aggtgcgaat
     4321 gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc aggagcagtg
     4381 gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa
     4441 ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg cgaattatag
     4501 aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac cggattagtc
     4561 ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg attgaaatta
     4621 ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa acacactgta
     4681 caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc tgcaggtgct
     4741 tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt catagataat
     4801 tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt caatctggaa
     4861 gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca agcggtactt
     4921 ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc gacctatcaa
     4981 gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca aaatgacgag
     5041 gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat cgatcagcct
     5101 ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg taagattctg
     5161 catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga aaatggacat
     5221 ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat tagagagtgt
     5281 ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa atatccgacg
     5341 agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa agacacttct
     5401 ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg agccgctgcg
     5461 aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc aagatgagta
     5521 atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag caattcaaaa
     5581 aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt tctgctttag
     5641 gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt tatgcttacc
     5701 ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag
     5761 taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc atacagaaac
     5821 aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa tatctgactg
     5881 ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac ctgattttct
     5941 ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca aaagcattta
     6001 aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca ggaaaagaaa
     6061 aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag atgttgcaag
     6121 cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat ccgcgaatca
     6181 tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag atggcttata
     6241 tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct gatatcccca
     6301 tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg aatcacgaaa
     6361 tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt tccatcactt
     6421 tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg agcatttttg
     6481 ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc ggagtgatcg
     6541 tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta aaagaaacac
     6601 ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc gttgggggtc
     6661 cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac aaattagaaa
     6721 ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc ggtgtaccga
     6781 ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat acggatgctg
     6841 aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga attcaagaca
     6901 aaaaaatatt tatatgaaca agtaaaaaac agcggggccg ttatccatga aatcggcgaa
     6961 acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt tcgaacagat
     7021 atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa gcatgaaggt
     7081 tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt gactgacttt
     7141 ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca gccatcagaa
     7201 gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa atatgaagta
     7261 caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt tttctctcga
     7321 ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg ccagtctgca
     7381 catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg atttttgaag
     7441 aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat gaaatttggc
     7501 caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt atccggaagt
     7561 atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga ccatttagca
     7621 gaacagtttc actctacagc tactatttct tttcgctata atgacggcta tccagctgta
     7681 atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca tgaattagat
     7741 gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc atgtgtattt
     7801 ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga ttttgatatg
     7861 gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga aaatcttctt
     7921 gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaaat tatccaatcg
     7981 atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg atcagcaaat
     8041 atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc caactcctga
     8101 acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact ataccggaat
     8161 gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc gttatggatt
     8221 aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag cgattgcctc
     8281 tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc ctgcttattc
     8341 aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag atacttctga
     8401 cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat atggatcagc
     8461 tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat tgtctgcaaa
     8521 acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta taagtgatga
     8581 agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt atcttccaga
     8641 acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt ggcgagtggg
     8701 ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc aatatctggt
     8761 gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa
     8821 agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg tccagcaatt
     8881 ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt tttgcaaact
     8941 cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg aacaggcaaa
     9001 agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata tccggatttc
     9061 ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag actttttaac
     9121 agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc
     9181 aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg attcataact
     9241 aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca aggaggtttc
     9301 atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc atcaatcgat
     9361 cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg aagcaggaga
     9421 ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg taacagatgt
     9481 aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc atattttaaa
     9541 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
     9601 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
     9661 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
     9721 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
     9781 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
     9841 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
     9901 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
     9961 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
    10021 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
    10081 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
    10141 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
    10201 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
    10261 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
    10321 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
    10381 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
    10441 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
    10501 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
    10561 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
    10621 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
    10681 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
    10741 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
    10801 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
    10861 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
    10921 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
    10981 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
    11041 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
    11101 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
    11161 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DANJMA010000069        11148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000069 DANJMA010000000
VERSION     DANJMA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            6..305
                     /locus_tag="PWU31_002340"
     CDS             6..305
                     /locus_tag="PWU31_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084826.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWU31_002341"
     CDS             378..1739
                     /locus_tag="PWU31_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL1084827.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWU31_002342"
     CDS             1761..2021
                     /locus_tag="PWU31_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084828.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWU31_002343"
     CDS             2035..2787
                     /locus_tag="PWU31_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL1084829.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWU31_002344"
     CDS             complement(2881..3486)
                     /locus_tag="PWU31_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1084830.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="PWU31_002345"
     CDS             complement(3542..4516)
                     /locus_tag="PWU31_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL1084831.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="PWU31_002346"
     CDS             complement(4894..5613)
                     /locus_tag="PWU31_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1084832.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="PWU31_002347"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="PWU31_002347"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL1084833.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="PWU31_002348"
     CDS             7164..8561
                     /locus_tag="PWU31_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1084834.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="PWU31_002349"
     CDS             8642..9082
                     /locus_tag="PWU31_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1084835.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="PWU31_002350"
     CDS             9229..9555
                     /locus_tag="PWU31_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1084836.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="PWU31_002351"
     CDS             9556..10305
                     /locus_tag="PWU31_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084837.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="PWU31_002352"
     CDS             complement(10331..10741)
                     /locus_tag="PWU31_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL1084838.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3536 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaatt
//
LOCUS       DANJMA010000070        10845 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000070 DANJMA010000000
VERSION     DANJMA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..183
                     /locus_tag="PWU31_002353"
     CDS             <1..183
                     /locus_tag="PWU31_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084839.1"
                     /translation="QHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKR
                     KNEIYYEQQLSQKERV"
     gene            244..1713
                     /locus_tag="PWU31_002354"
     CDS             244..1713
                     /locus_tag="PWU31_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL1084840.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1724..3733
                     /gene="topB"
                     /locus_tag="PWU31_002355"
     CDS             1724..3733
                     /gene="topB"
                     /locus_tag="PWU31_002355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1084841.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELYSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3849..3998
                     /locus_tag="PWU31_002356"
     CDS             3849..3998
                     /locus_tag="PWU31_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084842.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4079..4675
                     /locus_tag="PWU31_002357"
     CDS             4079..4675
                     /locus_tag="PWU31_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL1084843.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            4688..5587
                     /locus_tag="PWU31_002358"
     CDS             4688..5587
                     /locus_tag="PWU31_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL1084844.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            5590..5721
                     /gene="ssb"
                     /locus_tag="PWU31_002359"
     CDS             5590..5721
                     /gene="ssb"
                     /locus_tag="PWU31_002359"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1084845.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5743..6075
                     /gene="ssb"
                     /locus_tag="PWU31_002360"
     CDS             5743..6075
                     /gene="ssb"
                     /locus_tag="PWU31_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1084846.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            6097..6354
                     /locus_tag="PWU31_002361"
     CDS             6097..6354
                     /locus_tag="PWU31_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084847.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6513..6635)
                     /locus_tag="PWU31_002362"
     CDS             complement(6513..6635)
                     /locus_tag="PWU31_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL1084848.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6825..7049
                     /locus_tag="PWU31_002363"
     CDS             6825..7049
                     /locus_tag="PWU31_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084849.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            7497..7703
                     /locus_tag="PWU31_002364"
     CDS             7497..7703
                     /locus_tag="PWU31_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL1084850.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7703..7954
                     /locus_tag="PWU31_002365"
     CDS             7703..7954
                     /locus_tag="PWU31_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084851.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            7970..8407
                     /locus_tag="PWU31_002366"
     CDS             7970..8407
                     /locus_tag="PWU31_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084852.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            8400..9095
                     /locus_tag="PWU31_002367"
     CDS             8400..9095
                     /locus_tag="PWU31_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084853.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            9476..9655
                     /locus_tag="PWU31_002368"
                     /pseudo
     CDS             9476..9655
                     /locus_tag="PWU31_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9717..9901
                     /locus_tag="PWU31_002369"
                     /pseudo
     CDS             9717..9901
                     /locus_tag="PWU31_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            9935..10366
                     /locus_tag="PWU31_002370"
     CDS             9935..10366
                     /locus_tag="PWU31_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL1084854.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            complement(10540..>10845)
                     /locus_tag="PWU31_002371"
     CDS             complement(10540..>10845)
                     /locus_tag="PWU31_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1084855.1"
                     /translation="FKSITFDCGKEFSSWKSISNTNDIDIYFSDPGTPSQRGLNEHSN
                     GLLRKDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         3899 a         1829 c         2254 g         2863 t
ORIGIN      
        1 cagcataatc aagtaatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta
       61 ccttcagaag aatttgcgct aggaaattca ttccgatttt cacttgagac agtgtcagaa
      121 gtggcaaaac gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgtgta
      181 taaaacaagt aaaaaagact atctgaaaaa cggatagtct tttttctttt gggaggtttt
      241 aaaatgaccg agaaaaaaat gagtcttatt gatcgatgta aacagatcga tatagtggat
      301 tttgctcgaa acaatggaat ggctgtagtc aataaaggac gagactatcg cttagaagac
      361 catgattcat ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt
      421 agtggcgaca ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct
      481 aagcaacaat ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga
      541 gtggaaaatc ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca
      601 ctcacagaaa aaggaaggaa ttatctgaag gaagaacgaa aactaccaga gtggctgatt
      661 gattatgcag aaaaagaagg gttgattgct gaactaaagc cgaaacatga acggcaaaat
      721 tttttagttg gagacgaccg tttagatcat gcggttgctt ttttatggaa agatccacag
      781 acaggagaga cagtaggggc aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt
      841 aaacggggaa cgtataaaca cattgacaag aatcctacgc caaatcacgg atttaattta
      901 aaaataggcg atccgaagca tttaaaattc tttgaaagta gtattgatct gttaagttac
      961 gctgcattga atcgggagaa actacaagat gcctggttag tttcgatgga cgggttaaaa
     1021 catcatgtaa tcagtcatta cgttgaagag tcgatttctg aattaagacg aaaacaaaca
     1081 tttcctcaat cgattgaaat atgtgtggac aatgatcgtg caggacatat tttttatgaa
     1141 aaagaacaga tgaagggaat cgttgatcct tttaccaata agaaaatccg ttgtgagcga
     1201 ggaattccaa atgattggca agtacctaaa gaatacaaag ctacttatga agcagtcgca
     1261 aaagaaatga gtgttgaacc tgaagcaatt atggccatac acaaaactga aactaattta
     1321 cagctgacaa atcaattggt gtctgctcat gatgttcaat ctacgtttgg aaagatgttg
     1381 gcgaagggcg aaccagtaga aacaattgat ttgaaagaag catgtaccac ggtcgcgaag
     1441 gaactgaaag tatgtgaacg cgcagatgga acatacaact ttgatcgttt ttatagtaga
     1501 aaagccaaca taaaagacgt taatgcagga atccttcttt cctataaagc agaacaatac
     1561 tacaaaggct ataaaaagca tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat
     1621 gatcaattga agcatgagat ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg
     1681 tttcaacaag gcagacaaca agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt
     1741 agcagaaaaa ccgtcacaag cgaaggctta tgccgatagc ttttctaaag ctacccgaaa
     1801 agatgggtat tttgagatcc aagatcgatt gtttacagga gaaactgtga tcacgtatgg
     1861 ttttggtcat ttagtcgagt tatattcgcc cgatatgtac gatgaaaaat ggaaacaatg
     1921 ggcattagaa catttgccta ttttccccaa tcaataccac tatcatgttc ctaaagataa
     1981 gaaaaagcag tttagtgtcg tcaaacgaca gctgcagtca gctgatacga ttgtgatcgc
     2041 gactgatagc gatcgtgaag gggagctgat tgcctggtca atcattcaac aagcaggcgc
     2101 caatcaagga aaaaccttta aaagactttg gatcaactcc ttagaaaaag aagccattta
     2161 tcagggattc caacagttac gagacgcaga agagacctat ccaaaatttg aagaagtaca
     2221 agcacgtcag atcgccgatt ggttaattgg gatgaacggt agcccactgt atagtttact
     2281 cttgcagcaa aaaggcatac caggaagttt ttctttaggt agagtccaaa ccccaacact
     2341 ctatatgatt tatcaattgc aggaaaagat tagaaacttc caaaaagaac catattttga
     2401 aggaaaagcc caagtaatcg ctcaaaatgg cgcatttgat gcgaagcttg atccaaatga
     2461 aacccaagcg acacaagaag catttgaagc ctatttaaaa gaaaaaggtg ttcaattagg
     2521 aaaacagcca ggtacgatcc ttcaagtcga aacagagaaa aaaagtgcgg ctagcccacg
     2581 tcttttttct ttatcgagcc ttcagtctaa aatgaatcaa ctcatgaaag ctagtgccaa
     2641 agatactttg gaagccatgc aagggttgta tgaaggaaaa tatctaagct atccgcgaac
     2701 ggatacccct tatattacgg aaggggaata tgcgtatctg ttggatcact tagacgaata
     2761 caagcatttt ttaaaagccg aagcgattcc gactcctatt catacaccga atagtcggta
     2821 tgtgaataat aagaaagtcc aggaacatta cgcgatcatt cctacaaaaa cggttatgac
     2881 agcagcggct tttgaacaac taagtccact gcagcaggct atttatgaac aagtgttaaa
     2941 aacaacggtg gccatgtttg cggaaaagta tacctatgaa gaaacaacga tcttgacgca
     3001 agttcagcag cttcagttaa aggcaatcga aaaggtgccg ctagatctag gttggaagaa
     3061 gttatttgga aaagagagcg acggaaaaga gaaggaagaa gagccgctac ttcctaaagt
     3121 gacaaaagga gaaatggtga cggtggatct acaagttctt gaaaaagaaa ccaagccacc
     3181 acaaccttat accgaaggta cgttgatcac tgcaatgaaa acagctggga aaaccgtgga
     3241 tagtgaagaa gcgcaatcca tattgaaaga agtggaagga atcggtacag aagcgacacg
     3301 ggcgaatatt attgaaacct tgaaacaaaa ggaatatatc aaggtagaga aaaataagct
     3361 cgtggtgacg aataaaggga ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac
     3421 gagcgccgaa atgaccgcca aatgggaaag ctacttgtta aagatcggtg aacgaaaagg
     3481 aacacaaacc acttttctag ccaatatcca aaaatttgtc tctcacttat tggaagtcgt
     3541 accagggcaa atccaatcca ctgattttgg atcgacgtta caagaagtga aagcagccag
     3601 tgaaaagcaa gaagccgcgc gtcatttagg aatttgtcca aaatgccagg aacaagaagt
     3661 cttactctat caaaaggtgg cagcttgtac gtctgaagcc tgtgatttta aaaggtcaga
     3721 agggttcatt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     3781 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgccg cactgcaaaa taatggaggg
     3841 ttaatcttat gcgtaatacc acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     3901 ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa ttgaagaacc
     3961 agcagaggat tcaaaaagaa atcgaattag atcgataaaa tcttcctaac tattgtatac
     4021 gtataaactt atgttatact gtttttataa gtttatacgt atacaaagga gggagaaaat
     4081 gacaaaagca atggtataca agctgctaga agaatttaat ggaactttgg ctttgaagga
     4141 tgccaaaaaa gcgggcattt ctcctgtaac gatcaaacgt atggtagatc gaggggagct
     4201 ggatcgagag tatccaggtt ttttcacctt acctggtcaa tttcctgatg aattgtttat
     4261 ggcgcaaaaa aaatatgaac gcggcattat ttcacatatt accgcgttag atttgtatga
     4321 tttaacagat atgatcccta gacagatcga tctgactgta ccttacggtt atcacgtttc
     4381 tgaaaaagga ttgaaggaat tcgcagttga gctacactat acaaaagcgg aatggtatga
     4441 attaggtaag attgaaataa aaagtcgcta tgggaatcct attatagctt atgatccaga
     4501 aagaacttta tgtgacattt ggaatccctg gtataccgtt gaggatgaaa tcaaagtaaa
     4561 agcaatcaaa aactatatgg agtcggacag aaaaaatctt agaaaattga atgagtatcg
     4621 acgaatttta ccaacagaca aaacgatgcg ttcttatatt atggctttaa attaaaggag
     4681 aagatacatg acacctacgc agttaaaagc aaaagtgaag aatatttcta gagaaaaaga
     4741 agttgatccg cagcttgttt taagacattt tatgatggaa aaattcttgg aaaaaatctc
     4801 agagtctcct tatcgtgaaa attttgtgtt aaaaggcggc ttcttgattg gctcaaagta
     4861 tggcattgaa aatcgcacaa ccaaagatat tgatacgact ttacgggaaa tgaaagtaac
     4921 caaagaaaca ttaacaacgg ttctaaacga aattttttca acgccgacga aagaaggcat
     4981 tcaatttgag attcaaggaa tgaaagaaac gagagaagcc gattactatc caggatttag
     5041 cttacgtgtg ctggcacatt tggaaaacat gcggccagat tttaaggtag atgtgactac
     5101 aggtgactcg atctatcctg caacgatcac tcacagtcat aaattgatgt ttgaggatcg
     5161 aacgattcct ttggaatcct atcctacaga acaaattatt gctgaaaaat taagtgcaac
     5221 ctttgatttt ttaacagata attcgagagg aaaagatttt tatgatctct atactattcc
     5281 taaaatggag aagatcgatg agaagacctt gaaagatagt gtgagaaata catttacacg
     5341 tcgaggaaaa actgatccgt taaagatata ctatgaaaag gatatggatg tactgaaaaa
     5401 tagtcacgag ctaaaagata cctggaagaa atatcaaaaa agcaatcctt atgctgcgtc
     5461 gatcacgtac gatgagacaa tggattcgat tagtaatttg atggaaaaag tgattcacgt
     5521 cgaagaaaaa gagcgaaaac tagcttatca aagacaccaa cagatgaata gagaaatgga
     5581 gagataaaga tgattaataa tgtgacatta gtaggacgct taaccaaaga tcctgattta
     5641 cgttataccg caagtggtac tgcagtagcg acttttaccc tagccgtcaa ccgaaatttc
     5701 acgaatcaaa acggcaattg agaagccgat tttattaact gcgtgatttg gcgcaaacca
     5761 gcagaaacaa tggcgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt
     5821 caaacgcgga cttacgacaa ccagcaagga caacgtgtat atgtgactga agttgtagca
     5881 gataactttc aactattaga aagtaaagca gcgacagaga gccgtgcgca cgctgatcaa
     5941 tcgtccacca gtccaagcac cgccaccttt gaacaacgga atacagcgac gcctaacaac
     6001 aatggattga acgcttcaca gaatccattt ggtggacaat caatcgatat tagcgatgat
     6061 gatctaccgt tttagtttga gaaagaagga gtaagcatgg caaaaaaaat tagtctgacg
     6121 atggatctat tgatacccgt aattgcattt atagacggga tttctttggg cgtcactgaa
     6181 aatcctttgg taaatagaat tgcgaaagga tcactatttc ttgtattcgt gtatcttgtc
     6241 tgttcttatc ttttaaaaag agcaggaaag gataattcct tttggcgatt gactaaagtc
     6301 acagtagcag cgacagctgt tatacttacg gctattctac cgctttatat gtagagttaa
     6361 cgggaaatcg ttgaacttct tgcgaaatat tgctataatg aacatgaaaa agaggagttg
     6421 cgctaacaac ccctcattaa cagcaccgat taaagaacgg tggcagaata ttagttattt
     6481 ggaaataacc gctgacctgc taaagtagac ggttatttct ttttgtcatt tttaagcata
     6541 tttacaatca aactaatcaa agcaatggta aacataccaa aaccaagaat ggtttgaatt
     6601 gtctcatatg cggacaaaag gcttactcct ttcatagatt ttagcactta cattcataag
     6661 caccaccacc tttccagaag tcgccaccgt cttcaaactt tactgtcttg actattatag
     6721 catatctaaa atcgaataaa aattctcaat tttagctgtt tttgctataa ttttagcgag
     6781 gtgatcgtga tggaacaata tattggttta ctcgataaat tagggtgatc aagacacagc
     6841 cattgcttgt gcgatttaca ttacgcacga gtcatgaacc aatcaattgt gtagtggcca
     6901 atatagaaat agcggataaa ttgctgattt tggaagaagg caagtatact atttcagtga
     6961 caggtcactt caataaacga aatcaactag tcattgaatc aatgcaaatt agaaaccctg
     7021 atcattttac gagattaatg ggcatataaa taaaagaaga gctgcagtcc taaatgagga
     7081 ttgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt ttaggaaggc
     7141 gcaagcgcct tctcaattga aaaacctctc tctcactcca accgccaccg ccgttcgaga
     7201 agaaaggaag tgaacgttgc acgttcacta agataagggg tcttgcaaag caagacaaaa
     7261 tagaaaaaac ttcctctcac caaaaggttc gttcctgttc gctatctctt tattgatttg
     7321 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     7381 acgctgcacg cccttgtcct tgtcgctttt agttgattca ttctggccaa caaatcaata
     7441 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc
     7501 gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
     7561 catatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt
     7621 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat attttgattg
     7681 atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt
     7741 cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ctttacttat
     7801 tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga
     7861 agacctagtg aagattgcag gatatgaact gcattatcaa gaaaacatgg aaacttatga
     7921 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa
     7981 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
     8041 gacattccta tacaatgaga cacgtgttcc aaaaaaacat tataaggaaa gattgtcggt
     8101 agctgtggaa gaaatgattg aatcgggtgt cgaaatgaat ctcattgcga cctactatcg
     8161 aacgcttgaa gaactgaaaa agcaaaatgg acaatggttt ttccaagcct tactatgctt
     8221 ggaagcaggc gtcaagccaa acactatcaa accttctgaa taccaagctt tagaactaac
     8281 gtatgggaaa tttgtcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttggacta
     8341 cttagaaaat gtcaacaagt atggcgcatg ttacatcatg aaaaaggagg acaacgaaaa
     8401 tgaataaggt gatttatcaa ctaacaacgg aagatgtgtc acacttttta acaaacaaag
     8461 aacatttgaa tgaagtaatc agacggattc aaaaagactt tagttgtatg gaagaaatga
     8521 agtactttat tcaaagagtc gaagaacaat tgtatttgga aagtgaaacg agtcaaacga
     8581 aggttacagt cagcaataat tatttagacg tgtataaacg tcgtcctgac acattaaaaa
     8641 aaggagcgga gcttctacga ctagaaacag aaaaatatat catgaatgtt tacctagaaa
     8701 ggagtgaact ggtcgaaaac tttgtcagct acacgatttg caggaaacac tattactcag
     8761 aatcagaata ttattcaatt tttgagagca tggaattaat ggaagagcta aacagcaaag
     8821 atgcgacggt agacgattat taccttgtat ttactaaatt tttgacgtgt gggacggaat
     8881 ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc cgaagtattt ttgagggaat
     8941 atgtagaaga agtagataac ccaggcgacg actggtacac gactttaact gattttttag
     9001 atacctacac ttacgacgat acagaagaaa tccaaaatga acttgaccgt aaacgaatgc
     9061 catatcaacg atttgaaagg aaaaaaggtc aataaccatt tataccgaaa agtgagaatg
     9121 tgtgaaataa aaacaaaaaa ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg
     9181 ctccaaccgc ccccgccgct taggtggtgc gaaataagcg agcaaagccc gaaaacctaa
     9241 gagaaagaag gagatttata tgaacaaaat tattccctta ttattagtcc tatttgttgg
     9301 agaaatactt atgcgactta gtgcagctgt acatttaaac ctcgcagtat ttttggtaat
     9361 actaatgttt tgtttgatct tttggcgatt tgtattagcc ctcgtaagtc tactattcaa
     9421 gatgattgca gccatatttt tcataggatt atttcgatga aaggagagag taccaatgcc
     9481 aattgcaata ggaaataaac ggttacccgt gacgttggat gaaaagagac aaaaagaatt
     9541 gcagtaacca aagcagaagt acggcaaaag tgaatccagg attatgtgta ttgcgttaga
     9601 tttattgatt gcgcaagaaa aagcaggatt tgacatacca gcactcaaaa agtgatcgca
     9661 ctttgcgcac gtgttaatac gtgttataat tattttgtaa ggaggtatag tgcacaatgc
     9721 cacttacggg aaaagaaatg cttaagttat tgaaaaaaat ggctgggttg agcgaaggca
     9781 agagggattt catcatcatc tttataagga tggtgttaga atcactgttc ctgttcatgc
     9841 caatcaagat cttggccgag gacttgagcg aaaaatttta aaggatgcgg gattgaaata
     9901 atctcgcatt cgattaaaga aaggattgac tgaaatgtta agtgagcctt tagcaaaaac
     9961 ctatcctgct atttttagcc cagaagaagg tggtgggtac tttattgaat ttcctgatgt
    10021 tcaaggtgct tacacaggaa ttaacgaaga cgatatatct tatggaattg ctatggcaca
    10081 agaggtgtta ggtatggttt tagcagatta tattgagcat gaagatctac ttccagaacc
    10141 aacaccaatc aataagattt ctgttgaaga tgattctttt actactttaa ttcgtgtaga
    10201 tgttgccaaa tatttgaaag acacagaact tgtcaaaaaa accttaacaa ttcctcaatg
    10261 ggcggataag ctaggcaaaa gggctggcat aaatttttca gtccttctaa cagaatcaat
    10321 tgcagataaa gcagataaca ttcttcgttc tggaagaaat aattagaact caaaagggtc
    10381 agaaacttaa aaatctttgg cttctttttt tactacaaag atggcttctt taaattattt
    10441 gcctgattga tttaaaaaaa ttgcgcaaag actacgagct aattaagttg ctcgtggtct
    10501 ttttttatga aaacttttta gatcttatta tttgtcaaat taagcgagac aaaactttcc
    10561 atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa tttagtgatt ttctagggat
    10621 atggtttctt ttggacgcaa cggatgagat gaatccttga ttgacttggt tgaagtccat
    10681 ttcttttggt agtccatctt ttcggaggag tccgtttgaa tgttcattta atccccgttg
    10741 ggaaggcgtt cctgggtctg agaaataaat atcaatatca ttcgtattac taatactttt
    10801 ccaactagag aattcttttc cacaatcaaa ggtaattgat ttgaa
//
LOCUS       DANJMA010000071        10760 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000071 DANJMA010000000
VERSION     DANJMA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="PWU31_002372"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="PWU31_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="PWU31_002373"
     CDS             complement(1437..2279)
                     /locus_tag="PWU31_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1084856.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="PWU31_002374"
     CDS             2604..3020
                     /locus_tag="PWU31_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1084857.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="PWU31_002375"
     CDS             3017..3487
                     /locus_tag="PWU31_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084858.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="PWU31_002376"
     CDS             3501..4274
                     /locus_tag="PWU31_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084859.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="PWU31_002377"
     CDS             4288..5100
                     /locus_tag="PWU31_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1084860.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="PWU31_002378"
     CDS             5154..6119
                     /locus_tag="PWU31_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1084861.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="PWU31_002379"
     CDS             6256..8979
                     /locus_tag="PWU31_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1084862.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="PWU31_002380"
     CDS             8992..9624
                     /locus_tag="PWU31_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1084863.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="PWU31_002381"
     CDS             9829..10056
                     /locus_tag="PWU31_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL1084864.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="PWU31_002382"
     CDS             10125..10391
                     /locus_tag="PWU31_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1084865.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="PWU31_002383"
     CDS             10381..10650
                     /locus_tag="PWU31_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1084866.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DANJMA010000072        10456 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000072 DANJMA010000000
VERSION     DANJMA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(20..250)
                     /locus_tag="PWU31_002384"
     CDS             complement(20..250)
                     /locus_tag="PWU31_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084867.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            804..1046
                     /locus_tag="PWU31_002385"
     CDS             804..1046
                     /locus_tag="PWU31_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL1084868.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1397..1651
                     /locus_tag="PWU31_002386"
     CDS             1397..1651
                     /locus_tag="PWU31_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HDL1084869.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            1680..2939
                     /locus_tag="PWU31_002387"
     CDS             1680..2939
                     /locus_tag="PWU31_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1084870.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            2992..3888
                     /locus_tag="PWU31_002388"
     CDS             2992..3888
                     /locus_tag="PWU31_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084871.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            3899..4768
                     /locus_tag="PWU31_002389"
     CDS             3899..4768
                     /locus_tag="PWU31_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084872.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            4778..5453
                     /locus_tag="PWU31_002390"
                     /pseudo
     CDS             4778..5453
                     /locus_tag="PWU31_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            5659..7323
                     /gene="alsS"
                     /locus_tag="PWU31_002391"
     CDS             5659..7323
                     /gene="alsS"
                     /locus_tag="PWU31_002391"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL1084873.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            7336..8046
                     /gene="budA"
                     /locus_tag="PWU31_002392"
     CDS             7336..8046
                     /gene="budA"
                     /locus_tag="PWU31_002392"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL1084874.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            8176..8673
                     /locus_tag="PWU31_002393"
     CDS             8176..8673
                     /locus_tag="PWU31_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL1084875.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8858..9118
                     /locus_tag="PWU31_002394"
     CDS             8858..9118
                     /locus_tag="PWU31_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084876.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9480..9719)
                     /locus_tag="PWU31_002395"
     CDS             complement(9480..9719)
                     /locus_tag="PWU31_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084877.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            10005..10319
                     /locus_tag="PWU31_002396"
     CDS             10005..10319
                     /locus_tag="PWU31_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084878.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         3438 a         1591 c         2119 g         3308 t
ORIGIN      
        1 tcattaaaaa gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata
       61 tatatcgagt ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt
      121 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
      181 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      241 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      301 atatttgagg ggcaaaaagg gggcaaaaat gcgtttacct ttgatatatc tcagattgaa
      361 catagtgttt attttatgtc ggtattgttg atttatagct gttttgaggg tgttagatac
      421 ataatagaat atagtggttt tgtttacgtg cattacacgg aaaagctgct cgtatcaaag
      481 aaatccgtcg ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct
      541 tttttcttag catgtaattt gaacaccctg atccagtttt tttctttaat tattggtagg
      601 ggagaataat ggattagaat aaatctacct ataagtagta gtaaaaaaaa gagcactatg
      661 ataatatctt tcttaagagt aaccgcttta ataaaaaatc cagaatattc taacataaat
      721 ggagtattct tagaatcaaa aggagaaaga agatggttta tacttatacg gagcaagaaa
      781 aaatgaaagt ttgagacaca ttcatgaatg atggaaaagt aaaacaagta ccaagttcaa
      841 caaaaaagaa aaacatccta ttgaaagaag tcttgaaacg ctttgaccat ggagtgactt
      901 atacagaaac agaggttaat tcgatactgc tgaacgtatt ttctagtggc gattatgtcg
      961 aacaaagacg ttatctaatc acatttggct ttttcaaaag aagttcagac ggtcgggcat
     1021 atcagatgat gggaattgaa aattagaagt tttaatttat actcattgaa aaaagttcaa
     1081 gtatgcgata ttggttctac accgcatact tgaacttttt ttgtcatctg tatctataat
     1141 acaaagccaa gaacgttatt caaaatatgg atagctatga taaaagctaa tgagcgattc
     1201 gcagttaaat aagcaatcgt cagtaaaatc gttccaggag cgtacatgaa aaaatcaatt
     1261 ggatgcgtta catgaataaa gataattctc ttttatttgt acgaataaat accaaaacgt
     1321 acctcctaaa attagattca aagaagatag caaagaggaa aaagatagtt ctgcagcagc
     1381 tatagcggca tgtggattgc tagaactttc caaacaactg cctgtttcag atcctcgtca
     1441 tttcgattac gaagagatag caatcaaaat cttggaagaa ttacagaaaa actatacaac
     1501 gaaacgagtt aacggatcaa acggtctgct tctccatgcc gtttatgata aaaacagctt
     1561 aaaaggtgta gatgaatgtg tgatctgggg cgattacttt tatgtagaag gaatcactcg
     1621 tctagctaaa acatggtact gttactggtg atgtataaga gaagaggagg attcatataa
     1681 tgaagcgatg gaaaaaaatg atgattggat caatagtttt aggaatgtca ctcgtatttg
     1741 gtgcttgtgg taatcaagaa gacagtagtg aagaatcagc aaatgaaatt accgtttcga
     1801 catggaatta tgaaacaaca cctgaattca aagcgctctt tgaagcattc gaaaagaaaa
     1861 cgggaatcaa agtgaaagca gtagatattg cttcagatga ttatgatacg aaactcacca
     1921 cgatgctttc ttctggagac acgacagatg tattgacgat gaaaaatctt ttgtcgtatt
     1981 caaattatgc tttacgagat cagttagtcg atcaaacaga acgaattaga gaattagaca
     2041 ctgatgctgc tgaagaaaca tatgaaatgt atgatatcga tggaaaaaca tatgcattgc
     2101 catatcgaac ggacttttgg gtactatatt acaataagaa aatgtttgat gatgcaggaa
     2161 tcgaatatcc aaaaaatcta acttgggacg aatatgaaga tttagcaaaa aaacttagta
     2221 aaaatgacgg acaagtttac ggagcatatc aacatatctg gcgttcgaca atccaagcta
     2281 ttgcagctgc tcaaaacgag gcgaatcttg ttgaaccaga ttatcaatat atgactgatt
     2341 attatgatcg agcattgagg atgcaaaaag aaggcgcgca gatggatttt ggcacggcta
     2401 aatcaacgaa agtaacctat cagtctcaat ttgaagaaca aaaagcagca atgatgtaca
     2461 tgggaacttg gtatatggct ggcatactcg caaacaaaga agcaagtaag acagaagtag
     2521 aatggggaat tacagctatt cctcagaaga aaaaaggaga atcggtcacg acgtttggct
     2581 caccaacagc atttgctgtc aataaaaact caaagaaaca aaaagcggcg caagaattta
     2641 ttgaatttgc agccagtgaa gaaggagcaa aagtattagc aggagtagga gtagttcctt
     2701 catatcgtac ggatgagatt gatcagcttt attttaatct cgctggaatg ccaacggatg
     2761 aaatttctaa aaaagctttt tctcctgatg aaatcaaact tgaatttccg attgatacgt
     2821 atgggcctgc aatcgataag attttacaag aagaatatga cttagttttg gttggagatg
     2881 aaacgccgga aaaaggaacg gtaaatatgg agaaacgtgt agctgcggaa aaagaataat
     2941 catgaaagga gagaagccaa gagataaaac actcttggtt tcgtcatcga aatggagaat
     3001 agtataaata agaaagctac aaatcatttg cgtagaaaac gaacgtggat cgcttattcg
     3061 tttatcgccc cgaatttcat tggttttttc gtttttacat taataccaat cctgttttcg
     3121 cttgtactag catttatgaa atgggattct ttttcaacgc cagaatttgc tggactagat
     3181 aactttcgta aaatggccaa cgatgagaca ttttggattt ctttgaaaaa tactttttta
     3241 tataccgctg ctgtagtgcc tggcacactt gtttgttcgt tagggctagc tttactacta
     3301 aatcagaaaa ttagagggat gaagcttttt cgtacgatat ttttctttcc gtatgttact
     3361 tccttagtag caattgctgt tgtttggaat atgttgtttc atccaacagt gggacctatt
     3421 aatatctttt taagacgatt tattgaacat cctccaggtt ggacttcaag ttcaaattgg
     3481 gcgctttggg caattgtaat tgtcagtatt tggcgaggga tgggttatta catgattcta
     3541 taccttgcag gactacaaag tatccaaaaa gaactatatg aagcgtcttc gctagatgga
     3601 gcaaaccgtt ggaaacaatt tttgaatgtg acattacctt cgttgagacc aacaaccttc
     3661 tttgtaacga tcatgcttgt catcaattgt ttcaaggtgt ttgaccttgt gcaagtgatg
     3721 acggatggcg gccctggtcg agcgacaaat gtgctggttt accaaatcta caatgaagca
     3781 ttcaaaaaat ttgattttgg ttatgcttca gctatttcac ttgttttgtt cgtaattgtt
     3841 ttgggcgtta cggttgttca attttcttgg aataagcgtc gtacttagga ggtgtgatat
     3901 ggaagagagt gttcgaaatg aaagaaaaaa agcaaaaatt cgttcaaaaa acatgccctt
     3961 tattcaatgg ttgggtatga ttctgctgat tgtattagct ttattgacga tcattccttt
     4021 tttatggatg gtttctgctt cattcaaagc aaataatgaa gtgttaacga ttccgattca
     4081 atggatccct aaaacttggc atttagagaa ttatctggtt atttggtctc gtattccttt
     4141 agtgacattt tttaagaata cagctttttt aagcgttgtg atcacgttga ttcagttgtt
     4201 tacttctagt tttgctgttt acgggttttc tgaactaaat tttaaagggc gcgatatttt
     4261 atttttagca tatatcggca cgattgctgt cccttggcag tcttatatga tcccgcagtt
     4321 cattatgatg agaaaagtgg gtttaacaga tacgttgtgg tcgttggtgc tattacaagc
     4381 gttcaatgct tttggtgtgt tcttgttgaa gcaatattac agtagtatcc cagattcttt
     4441 atgtgagagt gcgcggatcg atggcttatc agagtgggaa atctatcgca agatcatttt
     4501 acctcttaca aaaccggcga ttgccagttt gacgatcatc acattcgtta atacatggaa
     4561 cgattatatg gggccattca tttatttgtc ttctacggaa aataaaacaa tccagttagg
     4621 gttgaaaatg tttattgggc tatttgatgc agagtacgca ttaatcatgg ctgcctcagt
     4681 tgtttctgtt cttcctgttg cttttatatt tttagcgatg caaaaatatt ttgtagaagg
     4741 gatcgcttca tcaggaatga aggaatagga ggtgattatg aaagagcagc gttttgacaa
     4801 aaatgtttat atggaattct tttattggtt gtatcaatca ttggcaagta gcctttgttt
     4861 ttggtgtgtc aatatcttgt atatagcagc tgcgttcctt ttgtcgatag atggaaggaa
     4921 tgtcttttcc tttatttttt ctttactttt tattggtcca ggttgcatcg tgatgatagg
     4981 tatgacagaa gaattagcaa aagaaaaaag tttttcacca gtacgtttgt tcataaagac
     5041 gtttcgccaa ttttggctaa agggattttt ttattggcta tttgcttgga tagttagtgt
     5101 gataatgatt tttgattgtt tcttttttat acgtttttct tatggaaaat ggttgattcc
     5161 gttgtttgtt ttacttgctt gttttacttg cttgtttatc agtttccttt agcatcaact
     5221 gctggtattt tcaggtgaga aatccagcgt cgaaaccaaa tcaagttcta cggatagcgt
     5281 tctactatac gttgaaaaaa tggtatgtga gtttactaga ttttttacta ttgacaagtt
     5341 tgtttctctt ctttttcgta aagccccaat ggtgtattct actaggtcct tctatcgtat
     5401 ttggtttgat ttacttcaac aatcgtaaat tgatgagaac tatggatctg tagactttat
     5461 aaattgaaac tttcaattaa gaatgacagt tttttatgta aaaactgtca ttctttttca
     5521 ttttttgaaa catatggcta aacaaatgaa ataaaacatg ctatactttt gaatgaaaca
     5581 ggtttagaaa ctgtatcact tatttgtgat tatttctaac taataattaa aaataaagca
     5641 taaaaaggac gatgttcaat ggctgaaaaa atgaaacaag gttcgaaatc tgtagtagaa
     5701 agtttgatta atcatcatgt tgattatgta ttcggtatac caggtgcaaa aatcgatggt
     5761 gtatttaatg aattagagga tcaaggtcct gaattaatcg tgactcgtca cgaacaaaat
     5821 gctgcattta tggcacaagc aatcggacgt attacaggag agccaggagt agttatcgca
     5881 acaagtggtc ctggtgctag taacttagct acaggtttgg taacagcgac agcagaaggt
     5941 gacccagttt tagcaattgc cggacaagta aaacgaagcg atctattgaa gttgacccat
     6001 caaagcatgg ataatgcagc actgttccag ccaatcacaa aatacagtgc agaaatccaa
     6061 gatccagaaa caatttcaga agtgattgcc aatgcttatc gtatggctaa gagttcaaaa
     6121 aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg atgcacctgt ccaaggtaat
     6181 gtcatcaagc cattgagcga tcctaaatta ggttctgctt ctgcagatga tatccgttac
     6241 ctagctgaac gcattagaga agcaaaacta ccggtactat tagtaggtat gcgaggatca
     6301 agtgaaaaag aaacgttagc catcaggcaa ttagttgaaa agacagcatt gcctgttgta
     6361 gagacattcc aagcagctgg agtgatctca cgtgaattag aagcgcattt ctttggtcgt
     6421 gtgggtttat ttagaaacca accaggagat atgcttttga aacgaagtga tctagttatt
     6481 gcaattggct atgatccaat cgaatatgaa gcaagaaact ggaatgcgga aaaagatgcg
     6541 cggatcattg tgatcgatga agcgccagct gaaatcgatc cttttatgca gcctgaaaga
     6601 gaattgatcg gtgatatctc agccactctt gatttattga caggttcatt agagcctcaa
     6661 caagtgtcag aagatgcaaa agaatattta gcatctcttc aagcaaaatt aacggagcgg
     6721 gacattgttc aatcaaaagg agaagcaggt atcttgcatc ctttagaagt tattaacacg
     6781 cttcagtcaa aagtaacaga tgatatgact gttaccgtag atgtaggtag tcattatatt
     6841 tggatggcac gccactttag aagttatgaa cctcgtcatt tgttattcag taatggtatg
     6901 caaacgcttg gtgttgcatt gccatgggcg atttctgctg cattggtgcg tccaaataca
     6961 cagatcgtat cagtttccgg cgatggggga tttttattct ctgcccagga tttagaaacg
     7021 gcggtacgta aaaaattgaa tatcgttcat ttgatttgga atgatggaca ctacaatatg
     7081 gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt tggtccagtt
     7141 gattttgtga aatatgcaga agcttttggc gctaaaggaa tccgagctac aagtgtagag
     7201 gaattagaaa aagctttaga agaaggattt gcgacagaag gacccgtaat tattgatatt
     7261 ccgattgatt accgtgataa tgagaaattg ggagaaacaa tcttaccaga tcaattctat
     7321 taaggaagtg tcgacatgac tgaaaaaatc ttataccagc acgggacatt aggagctttg
     7381 atggccggct tgatggatgg aacagaaacg attgctcata tattagaaaa aggaactctg
     7441 ggattgggaa cattgcatgg gcttgatggt gaagtcatct ttttagatgg cgtagcttat
     7501 caaggaagat cagatggctc agttgcccaa ttagatggaa gtgaacttac cccctatgca
     7561 gcaattacag attttacgcc agatactagt ttttctgtct caaaaactgc agatggagaa
     7621 caattgaaaa aagacatatt agtaagagaa gccggggaaa acctattttt ggcagtgaag
     7681 ataactggat tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga aaaaccttac
     7741 agaagactag caaaaatttc agagtctcaa ccagaattcc aacaaagcaa tgtccaagga
     7801 acattagtcg ggttctttac gcctgaatta ttccaaggag ttgctgcagc gggatttcac
     7861 ctgcatttta tcgatgatac acatacattt ggcggacatg taatggattt tgaagtggct
     7921 gaaggaaaag ttgagattag ccgcatctcg tcccttgtac agcattttcc agttgatgat
     7981 ccgacttttg ttcacgaaac gattgattat gctgatttag cagcagaaat ccaacaagca
     8041 gaatagaaaa agacagcgag atgcgttcaa ggtgattaac ctgacgtatc tcgttttttt
     8101 gaggagaaaa cctagcgttt acaaaaaaac tgagctataa tcttctccat agatcaaaaa
     8161 ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga aattttatta
     8221 ttacagcaac aaggattatt agaagcagaa gataactggt tagcaaattt agcaaattca
     8281 tctgcattgt tgaatgagac tttgcctgag actgtctttg caggatatta tttattcgat
     8341 ggtaatgaat tgattttagg accgttccaa gggcgagttt cttgcacacg tatcaagatg
     8401 ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa cattgatcgt tgatgatgtc
     8461 aaaacccatg caaattacat ttcctgcgat tcagccgcac aatcagaaat cgtagtgcca
     8521 atggtaaaag aaggtaattt aatcggtgtt ttagatatcg attgcggagt aacgaaaggg
     8581 tatgatgaaa tcgatcaagc atttctagaa aaatatgtag atcttttatt agcaaatttt
     8641 aatataaatg ctttttcatt tattaataac taaaatcgtc tattgaaaca aaaaagaaga
     8701 gaaatcaaga ataaaaagag ccggaaagac agtgggcatt tctgtttttc ggttttttta
     8761 tttttttata aaatcacaag tgttattgat tttcataatc atattttaat gttattatca
     8821 ttttaaataa tgaaaaaaca ttaaggatgg atgaaatatg aataacgaaa ctatcatttt
     8881 aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa aaagtgattg gagaagtaat
     8941 cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa
     9001 tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt atgagttgga
     9061 aacaacagaa tatctatata actatagcaa caaatatgaa catactttag tttcataaag
     9121 atttaaataa agtgccttca aaagcgagaa gaccaagctt ttgaaggcac tttatttagt
     9181 tggcagtgat tgttcatgat ttttgattta ttcaaaaggt tcgcctagag aatctgtctc
     9241 tgtaacgagg cctggtggag aaactcgata aaaacctata ctgccagaac tgtcctattt
     9301 tcggatagac aaactagtag ttttaaaaag atcatccgat ccaatcattt caaaaaatgc
     9361 aaaactgata tcaggatcat ttagctcagg cttgttatcg taaagcattt gtaaaggttg
     9421 catttagtta ggagtaaacg cgaaactatc atcattattt gctgaagaag atgaagaagc
     9481 taattcagag tttgtttgaa ataaaggaga cgtgctgaca gtttctttac cctgtttttt
     9541 gcagaggtag aaacttttat atcggaagtt tctaccggag aatttttctc atttgtagaa
     9601 attgttgaat cgatactatt cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa
     9661 gtcccacaag cagtaagtaa caataaattt gataaactga taaacagaat tcttttcatt
     9721 atgtacacac tccttatagt atcgtttagc atacacctat gctcagtatt aggcaaaatc
     9781 aatgcaaaac tatcaagtct tttatagtat ctattagtct tagtaataca tatatggaga
     9841 gggtgatatc ccagttcaaa ccagatacat tggttaccat ctgtattaac gattatgcct
     9901 ctaaacgctt tttttatgaa aaaatttata agtgaaaacg taggtatgat attatctaga
     9961 ggtaattttt ttgggtattt aaatagttgc taggaggata gcttttggat ttttacagta
    10021 attttatctt aataatagca atattattac ttctaaatat ttggtttttt gataagagta
    10081 gaaatgcagg gataggtttt agaaccaaac gaagtacgtc ttctgaaaaa aagtgggtat
    10141 attcacagac tattttttac ggaggggtta tttcaatcag tttactttct tcaacacttt
    10201 attcttttaa tgttatagac gtttccatgt caaattttat atctattatt ggaatcctta
    10261 tttctgcaat tattacacaa ctacttcttg tgtttgaaga gaagtcaaaa aataattaaa
    10321 gcaattatgc cttttagtac aggaattatt acttcttaca aatgtatagc gaatgtgaag
    10381 cctgctgttt taattgttct agaaaaactt ttgagtgaac aattatttta tgttaggatg
    10441 aattaatttc tctaga
//
LOCUS       DANJMA010000073         9812 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000073 DANJMA010000000
VERSION     DANJMA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..121
                     /locus_tag="PWU31_002397"
     CDS             <1..121
                     /locus_tag="PWU31_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1084879.1"
                     /translation="FIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            598..798
                     /locus_tag="PWU31_002398"
                     /pseudo
     CDS             598..798
                     /locus_tag="PWU31_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1510..2115)
                     /locus_tag="PWU31_002399"
     CDS             complement(1510..2115)
                     /locus_tag="PWU31_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1084880.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2297..2632
                     /locus_tag="PWU31_002400"
                     /pseudo
     CDS             2297..2632
                     /locus_tag="PWU31_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2797..3024
                     /locus_tag="PWU31_002401"
                     /pseudo
     CDS             2797..3024
                     /locus_tag="PWU31_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3150..4121
                     /locus_tag="PWU31_002402"
     CDS             3150..4121
                     /locus_tag="PWU31_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1084881.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4261..5574
                     /locus_tag="PWU31_002403"
     CDS             4261..5574
                     /locus_tag="PWU31_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1084882.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5617..6483
                     /locus_tag="PWU31_002404"
     CDS             5617..6483
                     /locus_tag="PWU31_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1084883.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6480..7328
                     /locus_tag="PWU31_002405"
     CDS             6480..7328
                     /locus_tag="PWU31_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1084884.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7342..8814
                     /locus_tag="PWU31_002406"
     CDS             7342..8814
                     /locus_tag="PWU31_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL1084885.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8831..9703
                     /locus_tag="PWU31_002407"
     CDS             8831..9703
                     /locus_tag="PWU31_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1084886.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3081 a         1679 c         2038 g         3014 t
ORIGIN      
        1 ttttattcaa tctgtcgcat ctaaacgaaa taatattcct agaaaatcac tggattatcg
       61 aacacctttg gaagtatttt tgagttacgt aagtattgat gatctgtcta acttaatttg
      121 acaattaaga catttaaaaa gaaaagtgac agataggaat aaaaacttat taaatgtagt
      181 tatgatctgt tatatttcat ttctaatact aggtcttcca caagcagtgt ttcctgaaat
      241 gttttctgga gactggggta ttgtcagcta ttctatatta acattgattg ctatggtttt
      301 aggtacttca ttttataatg cttttgtaag taagcatgtt gaggaatatg aagtattaat
      361 gtacttaaaa gagaaaaaaa caagtaatta atatgagaac accacgcata gtttttattt
      421 tgagatattc tagaatgaaa cacattaaga acgaaagcaa agcaaaagac gccagaactt
      481 ctactacctt ctaagttttt tcatatttgt ttgttatgct atacatagat ctgcataatt
      541 agacttgcgc tgcccgttat tgaagtttca ggcagcgctc tttttggggg atcgataatg
      601 gaagaagaaa aaatatttga gaaacgctgg cagctagcat caagtgaaca aagagcaaga
      661 tacaataatt tgatcacctc ctaccccact gttgactaga catataagga aaagaaatac
      721 ttactttggc tgtgccagtt agatatagat acctttgaaa catttgaagt gatcctagat
      781 aaaattaaac agaattgatc agaaacaagc gaagccttct ctcggttcgc ttgttttgct
      841 atttattagt tggctcaggg caactctaag ctcgtttgaa agaaaaccga tggataatcc
      901 ccttgtttgt accactttag aatacatgga tgtacgtatt gccattgact cctattagtt
      961 actgaaaaaa aatttaaaca agttgagtat tgcagataaa tagctccagt gaggtgaata
     1021 actgctccac tcaaggcaat agatagctcc atccagggca acaaactgct ccactttcat
     1081 atttaataga tgaataacct tctgtataat agaaaggcat acctttcggt atgacaactt
     1141 atacagaatg ttattttatt ggttcactat cgaaaaattt tggagcttta tttttccggt
     1201 atttctcaac gaacgattag tttaactgtg ggaagctcca gacatacgat tcgagaagtt
     1261 acttctcgag cagacaggaa gggggtgact gaactaaccg ctgatatgag cgatacttgg
     1321 ctgtaggaat atctttttcc ggaaaaacaa acaacagcac gaggttacct tcctgtagac
     1381 tgggaatatg ttcataaaga actaatgaaa aagaatatga cattgaaact ccttcataga
     1441 gaatatgggg tacatggaac atagaaacga aaatgcacgg tagaaaagcg aatgaatata
     1501 gtataaattc tacaaatcat tcgctttctt tattttatat atggttggtc gagagagacc
     1561 attttctttg gcaatttctg caactggaac tccttgattt aacatgtcca ccacagtttt
     1621 aaagactaat cgtttttgcg gatctgttga gttctcagaa tattcaattg gtcgcccctt
     1681 atatcgacca gcttttttgg ccaataatac tccttgccgc tgacgttcac ggattttctc
     1741 acgttcgttt tgtgcaatat aacttagcaa tgataaaaac atatcgaaca tagcagtatc
     1801 taggagttca tttccagtat taaaatctaa aaactgtgca tctagtattt ttaatgctac
     1861 ttttttctgt ttcataaagg taactgtatt ttttatgtct tcataatctc tgcctaggcg
     1921 atccagtgat gcaacaatga cacaatcacc ttctcggata taatttagta atttttgaaa
     1981 ctcaggtcgc tggatatctt taccactggc tttttcttgg aataatttat caatgttaag
     2041 gtcttccatc attgccaatt gtcgatctaa attttgatct tgtgaagaca ctctaatata
     2101 tccaattttt gccataaaaa ctccttttaa aaaatgatat aatttacatg taaaatttac
     2161 ttgtagtttt actgttatca tagcattttt aaaaataaat tttaagtcta cttataattt
     2221 actttggagg ttttgaatga atgaaattaa gcgcaataaa aattcttttg gtgtttattt
     2281 aggagagcct agctcggaac agttaaataa aggtgaagct cgtcatgctc tatgtcgaaa
     2341 tatattttat ggtagaaaag gcaaacttta tcaaacttat tacgacggta tggaggaaca
     2401 gctcaacgca ttaggattag taactaatgc tatcatatat tggaattcac tatatttaga
     2461 aaaagtcttt gaacaaatta aaacggaagg gttcgactgt tccgaagaag tgttacgcaa
     2521 gcaatttcca ttaattagtg atcacattaa ttttgttggc aaatatagtt tccgctatga
     2581 aaaagaatta tcgaatggaa tactacgtcc gttaaatata gagaattatt gagaatgttc
     2641 gtcttttggt tcctaccgtg cattttcgca cggatgttcc agatactatt tttgaatgta
     2701 taaaattgaa aacacaagaa tactaataaa tcaacgattc ttgtgttttt aatttttcac
     2761 taccgtgcat tttcgtttct atgttccatg taccccaatc ttgaaaaatt aattaaagac
     2821 ttagataaag ccattgacga cttagtcgtg gtgctaccgg agtatcaatg tttaaccagt
     2881 atcccaggta ttggtcctgt ttaggctgct ggaatactcg ctgaaattgg tccaattgaa
     2941 cgtttttctg atgaaataaa attgacaaag tatgccggcc tatattggcg acagagtcaa
     3001 tccggtaaat ctgaatacga aaattttttt acgaaaagct tcacgtctaa tatctatttt
     3061 aaaataaaaa tattcctgct aatttggaat cgtttccttc ttttggtata ataatacctg
     3121 aaattctttt gagaggagtt gttcgtgaag tgaaaactac tatgaaggat gttgctgcgt
     3181 tagcaggagt tggtgtgggt acggtatcta gagtaataaa tggagtaaag gtgaaagagg
     3241 ttaccaaaga aaaagtaatg gctgcaatta gtgagcttaa ttatgagcct gatgaatatg
     3301 ccagaggtat gaaaacgaat agtagcaata caatagctct tattttacct acgatttggc
     3361 atcccttctt ttccgagttt gcctactatg tagaggaagc tctcagccaa aaaaattata
     3421 agcttttttt gtgtaatgcg aacggcagtg cggaaaaaga aaaagaatat atccaaatgg
     3481 tcaagcagag taaagtagac ggaattatag gtattaccta ttcagacatt gataaatatg
     3541 tatcatcaaa tttaccattt gtcagtatag accgccattt ttcggaacag gtttcttatg
     3601 taacggcaga taattattta ggtgggcaaa ttgccgctca agaactactt actcgtaact
     3661 gtaaaaactt gtgctatgtt gggggtgttt caccttaccc taacgaaaca aacaatcgta
     3721 aagctggttt ttacaatatt tgtgaggaaa ataaaatagc tgtgaaggta ttggatatgc
     3781 ctgaaccaat tagcaattta gaggaacaat tgctcgcttt tttttcggat aatcctgaaa
     3841 ttgatagcat ttttaccatc aatgatttca tggctttgga tgtgataaaa gcccaaaaaa
     3901 aactagggaa ggaagcacct aaggattttc aaattattgg ttttgatgga gtcaagctat
     3961 cgttgcaaaa agactattta ctatcaacga ttgtacagcc gatagaggaa atggccaagg
     4021 tttcagtaga aacactcatg gcgatgattt taaatgaaga accgcctaag cgtgtggtag
     4081 taccggtttt ctttcacgct ggagagacga ctaaagagta aaaaaccggt ttataacgat
     4141 ttttttgaga taggttgaca gaaagtgatt tcaatgatat cttaaaacca attaattgga
     4201 aacgttacca attaattgaa aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg
     4261 atgaaaaaga gaatgttagc aattgcagca attttagcat taacagctgg aggacttgca
     4321 ggatgtggca gtaaggagaa aagttcaggt agcagtgatc caggagaaat taagatttgg
     4381 gttcagtttt ctgatgaaac gcctgaagga aaagcttggg cagagattgt gaacaatttt
     4441 aatgatgaat acgagggtga atataaagct gttactgagt atattcctcg tagcggaagc
     4501 ggcggtggat atgaggataa agtaaatgct gcaattacaa ccaataccct tcctgatgtt
     4561 attacgttag atggaccgaa tactgcagct tatgctcatt caggagtcat tgctccatta
     4621 gatgattatt tgaaagatgc ggacatggat gatgtattag acagcattat tcagcaagga
     4681 acctacgatg gcaaaatgta tgcgtttggt ttctcagagt ccagtgtggg tgtctattac
     4741 aataagtcgc tgttcaaaga tgctggaatt acagaagatc aattgccgac gttagaagat
     4801 ccttggactt ggacggaatt tcaagcaatt tgccaaaagc tgactgataa atacaaaacg
     4861 cctgcaattg atatgcaaat ggcgtcggga gacgaaatgc tgacctacgc atatacacca
     4921 tttatatggt cgaatggcgg cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat
     4981 tttaacggta aaaaatccgt tgaagcgttg gaatttattc aagaattggt tgaaaaaggt
     5041 tacaccacaa tcagtcctat tgaaaaagga tttgaaactg gcaaatatgc catgatgctg
     5101 agcggttctt ggacaatggc agatttagag gaaaactatc aagagattga ttatggtatt
     5161 ttgccttacc ccgtttcaga tgcaactggg gagttagttt caccaaccgg aagctggcag
     5221 ttagcaatga ctgagaaaac agagaaaaag gatgcttctg cggcctttat taagtttgca
     5281 accagtacag aatctagtcg tattatgagt ttagggaaca gtgttctacc tatcagaaat
     5341 tcaacagttg atttaattaa agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa
     5401 aaccaacttt ctggacatgc ccgcccagtg gttgtagctt atccgcaagt atctagagca
     5461 ttccaacaag cagttcaaga tattagctac ttcgaggaaa acccagatgt acaaaaagta
     5521 gcagatagtc gcgctgctga aatgcaacag gcaattgata cttcactaaa atagaagttt
     5581 ctaaaggggg ccaatcaagt gttaaaaaaa ccgacaatga aagacaattt gataggttac
     5641 ggctttctag cgccagcttt gctgctgctg acgatttttc tgatcatacc cgtaatcatg
     5701 gtaatttatt atgcctttac tgattattat ttgttaacgc cagatgctag acaatttatc
     5761 ggcttagaaa attttaagcg actgttcgct gatccaattt ttgtaaaaag tatttggaac
     5821 acggctaaat ttgttgtttg gattattccg gtccaaatag gggcagcttt aggaatggca
     5881 cttattatca acaaaaatcg taaaggaaat atctttttta aagtagcctt ttttgcgcca
     5941 gtcgtgatgt ccttagtcgt tatttcgatt ttatggttgt acttgttaaa tcctaatgaa
     6001 ggtctggtta atacgctatt tgcaaagatt ggaattgctt cgcagccttt tttgactagt
     6061 cccaaacaag caatgtatac gcttgttttc gtatcggcat ggcaaggtgc aggatatcag
     6121 atgctgctgt ttttaggggg aatgcaaaat attccgcaag acgtttatga ggcagccgaa
     6181 attgatggtt tttctaagtt tcagcaattt cgctatatca ccatgccatt attaaagccc
     6241 acggcgattt ttattttact taccacattg atttcggcct ttaaactaat cattcagcca
     6301 atggtaatga ctcaaggtgg accaatgaat tctactatga caatggttta ttacatttac
     6361 caaactggat ttacagaccg tatggtaggc tactccagct ccattgcgtt aatctttacc
     6421 actatgattg gtttaattac aattgctcaa agaaaattgg taaaggagga tgatccagca
     6481 tgaccaagaa atttggaccg ctaatgatcg tagaatattg tttgctgttc cttttagcgg
     6541 cattattcat tttcccgatg ctgtggatgg tagtttcctc tatgaagcct gaagcagatg
     6601 tctatcaaaa tttagcttcc ttcaaagctt ttttacctag tttgaatcca gctaattggt
     6661 ttaaaactta tcaagaggtt atttcccggt tcagtgtggg aacttattta ttaaacagta
     6721 ttttttatgg tttaaccttt gcctgtgggt caatcattgt aaactctttg gcgggttttg
     6781 cctttgcgaa ggttaatttt tcaggcaaga aaattatctt tggagctttg ttagctctgc
     6841 tgattgtccc gatggagacg gttttaattc cgcaatttac catcgttaat tcactaggct
     6901 tagtgaatac tcgcttggca gtgattttac cagcgatggc cagcgcgttt aatatctatc
     6961 tgtttagaaa tttctttatc gcgattccag aagaaatcat tgaatcagcg aaattagatg
     7021 gtgcaaatat tgttaaaatt ttctttcaca tcatgctgcc aatgtctaaa ccagccatag
     7081 ccacagtagg tgtcttatcc tttatctcca gctggaatga ttatatttgg ccgttaatgg
     7141 tcttaactga cagcagcaaa ttttcaatgc aagtggcaat tactacgatc aatactacac
     7201 agccagttta tatcaatcaa gtgatggctg ttttaaccat tgcaacgatt ccactgatta
     7261 ttgtttatat cgttgcacaa aaatatattc tgcagggact tggcggttct ggaacgggta
     7321 taaaatagga gtgaatagtg aatgttgcaa aaacttttaa aggccaatga gtttattgag
     7381 aaaaatgctc aaacagtcaa tcaagaatac cggaataaat ttcatttgat ggctccgatt
     7441 ggctggatga atgatccaaa tggttttgtt tattatcaag aggagtatca cttgttttat
     7501 caatattatc cttacgacag catctgggga ccaatgcatt ggggccatgc taaaagcaag
     7561 gatctaattc actgggagca tttgccagta gcactggcac caggtgaaag ctatgacaag
     7621 gaaggctgtt tttccggcag tgccatagaa aaggatggca agctgtattt gatttataca
     7681 ggacacaatg ttgtggaagg gcaagttcga caagttcagt gtttggcagt ttcagaagat
     7741 ggcattcact ttgaaaaata tgccggaaat ccaatcatcg cagaagatca cttagcttct
     7801 gttgcaacga ccgaagattt ccgcgatccc aaagtgtttc agcgaaaaga agactatttt
     7861 actgtagtcg ctactaaaac tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa
     7921 gatttactaa cttgggagtt ttattccgta ttgctggagg gaactccaga acaaggcatt
     7981 gtatgggaat gcccagactt gtttgaacta gatggcaaag aagtcttgat tctttctcct
     8041 attcaaatga ctccacaagg caatgcttac caaaatacga actcaactgt tgctttcatc
     8101 ggtactgtcg attggcaaac aggcaaattt ctagtagaga attttcatga aatcgatggc
     8161 ggtttagatt tttatgcacc ccagacctgt gaaaacgaag caggccaaag aataatgatt
     8221 gcttggatgc aaatgtggca gcgtaaaata cctacgaatg atctgggcca tggttggtca
     8281 ggttccatga ccttgcctag agagctgagg gtggctgata atcaattgtt gcaacggcca
     8341 gtttcatcaa tttatcaagt aattgatccg cagaaaagga ttgaatgggc gaagactgaa
     8401 gagaatcgct tcttactgaa ggacttagta tatgaccagc actatttacg cttggaaatc
     8461 aatttaaaac aggcaacgaa actagaactt cactatgcga agggacccga ccatacatta
     8521 ctgcttaact atgatgttga acgggagtgc tttactgctt ccagggaaca catgccgtat
     8581 gatttatcag gagctgaaaa ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa
     8641 aataattgtc taaccatcga aattttccgt gatacttcag ctattgagct ttttctaaat
     8701 gatcgtctga ctatgagtac aactttttat gagacagaaa aaggacagga ttttcttttg
     8761 ctggcagaca gtgaagtaac cttttcattg gaaagcggct taattaatct atagaaggga
     8821 gagctcagtg atgatttatg gttcaatcga agcaggcggc acaaaatttg tgtgtgccat
     8881 tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga cacctgagga
     8941 aacaatacct ttagtcattg acttcttcaa acaataccaa gcagatttag cagggattgg
     9001 gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg ggtacattac
     9061 ctcgaccccg aaagtggcat ggcaaaattt tgatttcatc ggtaccatga aaaaagaatt
     9121 tccgatcccg atttcttgga caacagatgt caatgcggct gcctatggtg agtatgtctt
     9181 cggcagtggc aaaggattga gcagtgtcgt ttactatacg atcggtacgg gtgttggcgg
     9241 cggtgcctta caagaagggc gctttatcga agggtttagt catccagaaa tggggcatat
     9301 gctcgtcgtc cctcatccaa aagatgactt tgctggcagc tgtccatttc atggcaactg
     9361 tttagaaggg atggccgctg gacccgccat cgaaaaacgc ttagggaaaa aaggacaaga
     9421 ggttgcagaa gatgatcctt attgggaaat cgaagcctcc tatattgctc aatgtgccta
     9481 caatacaacc ttgatgtttt ctccagatgt gatcattttt ggcggtggtg tgatgaaaca
     9541 acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa ttggtcaatg ggtatgtcga
     9601 aacacctgag ttaagcaaat atatcgtgac cccagcatta gaagacaacg ctggaacgct
     9661 cggttgtttg gcattggctc gagaagcggt cttacattca taaacgaaaa aaggctcttc
     9721 gacatttagt gttggtagag aaagatacaa ctgaaaattt agtcataacc agttaggcag
     9781 agtgcttaac tggtttttgt tttacttgga ag
//
LOCUS       DANJMA010000074         9563 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000074 DANJMA010000000
VERSION     DANJMA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..888
                     /locus_tag="PWU31_002408"
     CDS             <1..888
                     /locus_tag="PWU31_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL1084887.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(930..1622)
                     /locus_tag="PWU31_002409"
     CDS             complement(930..1622)
                     /locus_tag="PWU31_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1084888.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1617..1883
                     /locus_tag="PWU31_002410"
     CDS             1617..1883
                     /locus_tag="PWU31_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1084889.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(2059..2628)
                     /locus_tag="PWU31_002411"
     CDS             complement(2059..2628)
                     /locus_tag="PWU31_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1084890.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2702..3991)
                     /locus_tag="PWU31_002412"
     CDS             complement(2702..3991)
                     /locus_tag="PWU31_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084891.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            4345..5193
                     /locus_tag="PWU31_002413"
     CDS             4345..5193
                     /locus_tag="PWU31_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL1084892.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5338..6279
                     /locus_tag="PWU31_002414"
     CDS             5338..6279
                     /locus_tag="PWU31_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL1084893.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6269..8104
                     /locus_tag="PWU31_002415"
                     /pseudo
     CDS             6269..8104
                     /locus_tag="PWU31_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8115..9074)
                     /locus_tag="PWU31_002416"
     CDS             complement(8115..9074)
                     /locus_tag="PWU31_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL1084894.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         2965 a         1798 c         1710 g         3090 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acaaagaggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc
      961 ttgtcatgaa aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc
     1021 atcatgacgt atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca
     1081 acatttccta aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt
     1141 actctagcta gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa
     1201 ttagcaatgt gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca
     1261 atgtgggcgt aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga
     1321 tcaattactg gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa
     1381 tcataggcta gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta
     1441 tgatcgcccc aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
     1501 ggagtgtcta atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg
     1561 tatactttat agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc
     1621 acaggacttt tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa
     1681 ggaaaacacc aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt
     1741 tagaagatat caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc
     1801 atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc
     1861 aaataaaaaa gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact
     1921 aattttattt ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc
     1981 ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga
     2041 agtgcgaaca tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc
     2101 catcaattga gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg
     2161 cggttttctt cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc
     2221 cacgactccg ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc
     2281 tagatttttt gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag
     2341 ctcttccatc ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt
     2401 tatttctttt ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg
     2461 caagagcgtt ttgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa
     2521 tatatatacg ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt
     2581 cacttctgtg atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc
     2641 tttactaatc gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt
     2701 tttatcggtt tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg
     2761 aaggatggta cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg
     2821 tgatcactag atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc
     2881 gttttcgatg tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt
     2941 catagcgaaa aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt
     3001 cctggagtgt tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt
     3061 ggatcgctag ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt
     3121 cctgataata acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagcc
     3181 attcttggta aagtactttt atcagctttg atttgctttc gctctcaaat tcgctattca
     3241 gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa tttccgatag tcttgtgtct
     3301 cttctatccg tttccaccgc ttttcagtaa tctggttaca gctcaagcgc aaaattgcta
     3361 gataattttt ttcttttatt ggcaaaatct gctcctctct aaatagcaat tcaggaaaca
     3421 attcaaagaa tagtgttcga ctgtctccta aaatctcgat ttctgccgga tcctttcttt
     3481 gcttcaataa ttggatgaac atctcagaaa aggactgctc tccatcatct gcaaaaaaat
     3541 gcggaaaatc tttcaaaaag taaaacagca gatatcttac ttttctttct gaaccaaaga
     3601 tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc aaaccacttt ctcaatcgct
     3661 cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt taatagcaga tttttcagta
     3721 aacaaaacgg gggtgattgg atcagaaaaa caaacaagag ataaggttcg ctatatcctt
     3781 gatcataaac taactgaacg gttttcttat gaagaatcag ctgaatggga cagtcgcttt
     3841 ccgccaaacg tttcagatgc cgatccactg ttttaacatc gattttcagt tcttcagcta
     3901 aatcagcttt tcgccagatc aaatctttca ttagattgat tagtgaaaat aaattctgtg
     3961 cattttttga taatagtttt tgccatctca tcgatcattt ctcacctgat ttctaaacat
     4021 attgtttgat caaaatgagt tcactcctaa aaagataaaa aactcccttt ctatttattt
     4081 aaatacgcaa atcttcctca ttatttttta ttttactcaa tttaatcgta ttctcatttc
     4141 aaaacgaatg tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct
     4201 tccattacaa tgaaataaat agcgatttta tgagaaggtg atgtgaaaat ggaagaaaaa
     4261 atagaagaat ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc
     4321 ggcaaacgtt caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta
     4381 ttcgaagata ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta
     4441 cagggaacct tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt
     4501 ttttccgtat acaattcaca atcaaaagag tttgaatttc gtccgggacc gatctttacc
     4561 acgatcttgt tggcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta
     4621 gaagcaatgg ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat
     4681 ttttttgtct tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa
     4741 gcacagctcg atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa
     4801 cttttgctac ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac
     4861 agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg
     4921 gcatcttatg ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc
     4981 atcagtccac gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat
     5041 gggagaaatt acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat
     5101 cgcctgattt tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact
     5161 cttgttgcac aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa
     5221 agattatttc attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct
     5281 tgtattcaac aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg
     5341 gagcttgatt tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc
     5401 actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt
     5461 ttctttacct ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa
     5521 gagattttga ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa
     5581 agaggatgcc atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc
     5641 agaaaaagtg caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag
     5701 attcaagcag aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa
     5761 agaggtcttg attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc
     5821 gattggaaat tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa
     5881 gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg
     5941 ctagacttgt atttttcttt atggcatttg agaaaagaaa agcagccgga acttctgata
     6001 ttaggcaacc gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa
     6061 gctggcgatg agaaagcttt tttcgcacac ttgaaaaaat atgcaaaaaa acaaatcgtc
     6121 ctctttgttc ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat
     6181 cgaacggctt atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag
     6241 atatgttcat tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg
     6301 cgtttactcc tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt
     6361 gcaaaacagc taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat
     6421 tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt
     6481 atattttcct tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac
     6541 agaggaattc ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt
     6601 ttttctcata atgttgctga tctatttatt tattacattg accgtggatt acgactattg
     6661 gtacgcaccc tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa
     6721 agatttactg tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag
     6781 gcaatttttc tccatccggt tgttgtcggg aaacactcga ttccagtcat tgactacagt
     6841 tctgcttatt tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca
     6901 ccaatctctc gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta
     6961 tgacacactc catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga
     7021 tgaacagctt ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaaa
     7081 aggaaatggc tattggaaga tcgaaaataa aaatcattac accggttctg gatggataga
     7141 agatcaggac gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca
     7201 aaccgttata gaagacaaag aaaacattac attgacccta aaaaaacaag ctttcttacc
     7261 ttatccttat ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta
     7321 ctatttgcct gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac
     7381 tttacaaaca gaaaggttag tttttacgag agaagaatta aaaagcagtc agttaaaagc
     7441 tgatgattta gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat
     7501 tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc
     7561 tgtctaacag tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca
     7621 tactccgaaa gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg
     7681 tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg
     7741 gacaaaaggt tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata
     7801 cagcattctc aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg
     7861 gcttccattt gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga
     7921 aacaaccgaa accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga
     7981 aactatttcc agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc
     8041 ttataaaatc gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt
     8101 gttattatga tttgtcaaat taagctagaa taaaatgctt cttgtacata gctcaaaaat
     8161 atttcgattg gtgttctgta attcaatgat tttcttggaa tatgattacg ttgattgctg
     8221 acactggaaa taaatgtctg attcactttt ctaaaatcca ttgatttcgg cagtccatta
     8281 cgacgcagaa tcccgttaga attctcgttt aatgggcgtt gagaaggtgt tccagggtcc
     8341 gcaaaatata tatcaatatc atgttggtta ctaatggctt tccagttaga aaattctttt
     8401 ccacagtcaa acgtaataga tttaaagaag tttttaggga agcgagaaaa ccattgatta
     8461 agggcagttt caatatctaa tgccttacgg ccgttgggtt taatcgtgat aatgacttta
     8521 gataatcttt caactaaagt aatgacggca cttttatgat gaatgccaac gatcgtatca
     8581 ccttcaaggt gaccaaactc agaattgaaa tcaggataat tatcaggtcg atcatggatt
     8641 gagcgctgat actgttgttt tccccgtttt tcctgatggc cattgggttt tcttttacct
     8701 ttcatcggta gtgtgtcaat atcaaatatt cctttagaaa ataaacgata aagtgttctc
     8761 ataccgcatg aaacaggcct ttcttttcgc ccgataatga cgtcaggcgt ccaaccaaga
     8821 gtgactttct ctttaatgta atctacttca tgagcaggta attgaatgac ttttctacca
     8881 cattttttct tattttcttt atactggtgc caataatcaa gagcagtctt gccttgcttg
     8941 aacttattga ctacattata aatagtttgt atagcacgtc ccattcggtt agcgatttca
     9001 accggcttat tatgttgaag ataatatgat tctatcattg tcagttcgtc tatggtaaga
     9061 tgtttgtagg tcatttatgg ttacactcct ttgttttctt tcgtcggaaa tacaatttga
     9121 gtgtaccata aatgcctttt tatttttcta acttaatttt acaattcgcg atatattaaa
     9181 caacgttgtc gcgcttcatt gaaacatttt atgaaaattc caaaaaagag ttgacacatg
     9241 tttttagacg tgataagata actaagtcgt taaaaagaac cacttgcgaa gcttgattct
     9301 atcgaaaaaa aacttcaaaa aagttcttga caaataacaa acgataagtt atgataaatg
     9361 agttgctgaa agatttcgaa aaacgaaatc gaaaaaaact tttaaaaaag agttgacaat
     9421 cgcttgtctc tctgatatga tataaaagtt gctacggcag ccaaaaacga atcgggaaac
     9481 actcccgatg aagtagatct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg
     9541 cgtcttgatt caattcaaga caa
//
LOCUS       DANJMA010000075         9339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000075 DANJMA010000000
VERSION     DANJMA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..617
                     /locus_tag="PWU31_002417"
     CDS             <1..617
                     /locus_tag="PWU31_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1084895.1"
                     /translation="VLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGA
                     VQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAE
                     KWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQN
                     EESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            995..3037
                     /locus_tag="PWU31_002418"
     CDS             995..3037
                     /locus_tag="PWU31_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084896.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3048..3668
                     /locus_tag="PWU31_002419"
     CDS             3048..3668
                     /locus_tag="PWU31_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL1084897.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3680..4138
                     /locus_tag="PWU31_002420"
     CDS             3680..4138
                     /locus_tag="PWU31_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084898.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4157..4435
                     /locus_tag="PWU31_002421"
     CDS             4157..4435
                     /locus_tag="PWU31_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084899.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4460..5827
                     /locus_tag="PWU31_002422"
     CDS             4460..5827
                     /locus_tag="PWU31_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL1084900.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5842..6987
                     /gene="dgoD"
                     /locus_tag="PWU31_002423"
     CDS             5842..6987
                     /gene="dgoD"
                     /locus_tag="PWU31_002423"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDL1084901.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7014..7505
                     /locus_tag="PWU31_002424"
     CDS             7014..7505
                     /locus_tag="PWU31_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084902.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7948..9147
                     /locus_tag="PWU31_002425"
     CDS             7948..9147
                     /locus_tag="PWU31_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1084903.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2897 a         1561 c         1991 g         2890 t
ORIGIN      
        1 aagtgttgag ctatgcgatt gcaccaactg aatctatcac gatttgggaa gagattttaa
       61 tagacctcca ggaacgtggc gtgaaaaacg tcctcctttt catcacggat ggcttaaagg
      121 ggatggcagg ggccgtgcag cggttctacc ccaaagcccg ttttcaacat tgctgtgtgc
      181 acgtttcccg taatatcagt cacaaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg
      241 attttaaaat ggtgtatcaa gcgtcatcta aaaaggcggc attggaagca cgtggtgctt
      301 ttgcggaaaa atggaaaacc agttatccaa aaatggtgga atcgatcctt tcgaacgatt
      361 acctgctcac tttctatgat ttcccattag ccatacgcaa gactatttac tctacgaact
      421 tgattgaatc ctttaataag caaatcaaga aatacagcca ccgcaaggaa cagttccaaa
      481 atgaagagtc gatggaacgt ttcttagtct cgtcttttga tacttacaac caaaaattcc
      541 taggtcgcag tcataaaggt ttccaacagg ccgaaggcga gctcgaacaa atgctgagtc
      601 aaccgatgga gaattagaaa caatctacag tagggaagaa ccatttacac aaaattattg
      661 acgctcccaa aagcattgtt tttagcagtt taatattatt ctttttcaat tagactgact
      721 tgatttagtt acggaataga tactttaatg tgttgaagaa gcaacgtccc gtgtttattt
      781 actaaagcat ccaatattct accgaatatt taatattcac attagcccta aaataattgt
      841 cataccatgt tacatttgat ataacagcgt tcattttaaa aatagctgtt agtgtgacaa
      901 aacattaaac caagtgtgac aaaaaacgtg atgaaagctt tccggaaacg tatgtgaaca
      961 agtatataat gattttgtca ggaggatagt caaaatgaat aaacgaattc gtgaaatact
     1021 caaactgatt attaaaaacc ctgaaatgaa actttccact ttaacaagtg aacttgattt
     1081 gacacgtcgt caaatcaatt acgcgatcaa tcaatttaat gaagaattga aaacaaagaa
     1141 cattccgtta attcagagaa gtcattctgg aaatattacg gtaccaatcg aggttatcca
     1201 aatgatatca cagcctgatt acgacatagg tgatgatcag gaacttgtcg ttctcactga
     1261 tagtgaaaga caagcattga tagtgatgac tctgatcgct aatatcgatt acatcagtct
     1321 ggatcatttg gtagatgcgg ttgatgttag taagacgact gttatggaag atatcaaacg
     1381 aacggaaccg ttcatcagaa attatagtct gacaattcga tataatcgga tgaatggtta
     1441 ccaattggtt ggttcggaac accgtatctt acagctgtta tcggatctag taaaacggtt
     1501 tccgattttt cgccgagaaa tgattcgcag aaaattggca caagatgttt ctgaagaaga
     1561 aatcgtccat ttgatccata acatggaaca aatgttgcat cttagttatt ctgatgagtc
     1621 ggtagactat ttgcagacat cagcgagatt tttagtaagc cgtgggatca agatgtcaga
     1681 taagcacgcg ttttttcccg atgatgtgac ggatactcct gaattccgaa tgctgatgat
     1741 cttggtacat gaaacaggtt ggagtctatc caaaagttat atggagtggt tcacgttatt
     1801 atttttaact tccaatattt ttgagagcaa aactactcaa aattatgatt cggatcaaga
     1861 attgcgaaaa ttgatcagcc aaatggttga aaatttccaa aatcaaacgt tgattttgat
     1921 cgatgaccga gaagactttg aacgtaggat actgagtcat ctgcgtccag cctgttttcg
     1981 aatacgctat aacttgagtt taggagttta ttcaatagat agtctgatca aggatagtaa
     2041 tcatgcgatt ttgattgatt tgatgaaaga attgattatt cctattgaga attggctaaa
     2101 taaggccttt ccaaatgatg aattagatct tttatcctat tactttggct atcaattaag
     2161 taatcataat aaattgaata agcaacgaca aagggctgta gttgtttgta ctaatggtgt
     2221 catggtctca aagttagtac gagccaatat ggaaaaactt tttccagaga tccatttttt
     2281 agcttcccta tctgtgcgtg atttctacaa atttgaagct gattatgatc tggtctttac
     2341 tacgacgcca ctaaaaagtt ctatgctgca gtttatgatc gagccaatca tgacatatca
     2401 gcaacaaatc agtttacgat atcgagtttt gagtgattta ggaattacta aggttgatcg
     2461 agcaatggat gaattgttaa caatcattag gaaatatgca aatatcacca atccagtttc
     2521 gttgcgagaa gaactgcagt actttttgat caaggaagat ggaagcacgc cgctggacaa
     2581 cttcaatgta ttgccttcat tgacttatta cttgcggccg agttatgtac aaatcgttga
     2641 agaagagatg tcttgggaac gagcagtgga ggtggcttgc cgaccgttat tggaacatca
     2701 aatcattgac tcgcgtttta tcgctgattg cttggatcaa atcagacaac cgggatatgc
     2761 aggttacttg ggaatgaaga cttgcattcc ccatacaacg gtcgataaag gtgtaatcag
     2821 tgacggtgtc agcattttag tttgtaaaaa gccgatacga tttcctaatg gtgaagatgt
     2881 cagcttgatt ttgccgcttt cattttttga tttaacgaag catctgcgag ccatcaatca
     2941 aatcgctgat atcgccaaag atgattcatt gattgagcag ttattgattg ctgatgacga
     3001 aaagacagtt tatcaactat taagacaaag atcttaagga ggaaaacatg agactagcag
     3061 attatcctaa attgacagtg atcatgcgtg gttatacttt tgatcaggca gatgctattt
     3121 tacaagcaat gcaggaattt gaccatcaat ttgccgtaga gatgacaatg aacacaaaag
     3181 gagctcttga aaatatacat gaactaaatc aacgttatgg tgacacgact tatatcggcg
     3241 ctgggacggt aaggacatta gaacaagccc aagcctgcta tgaggctggt gcaaaatttt
     3301 tattaggacc acatatgttc acaaaagaaa tgttgtctta cgccaataag aaaggattat
     3361 tggcggtacc tgccgcaatg actccttcag aagtcgaccg tatgtttaaa gaaggagctg
     3421 atattgtaaa agtatttcct gcagcggttg ttactccacg ttttttcaag gatattcaag
     3481 cacctttagg taagttacca ctaatggggg ttggtggaat cagtaaggag aatgcgaaag
     3541 agttttttga acaaggtgct agctatttag gtcttggttc cggtatgttc aacaagcaag
     3601 acatcgaaga gctaaatgtt aaaaacttag cacgttcgat gaaagaatta attgaggcta
     3661 ttaaatagga gtgaggactt tggaacttta tttgaatgag gaactcgttt ttcgaaatgt
     3721 tcaagcaaaa acagatagtg aagtcttagc gtttttagca agcgagatgt acaaaaaaaa
     3781 ctacgtgaag gaggaatata ttcaagcaat ccaagaacgg gaaaaagaat accctactgg
     3841 attaccatca acaccaccag gaatcgcaat tccccatgca aactatgaaa tggtcaataa
     3901 aacaacttta gcaattgcga ctcttaaaga tccggtccta tttcataata tggaagataa
     3961 caatgagcag ttaccaatcc aaattgtgat catgatggcg attggggaac cacatgggca
     4021 agttgaaatg ttacaaaaaa ttgttggaat catacaagat gagccactgc ggcaagaaat
     4081 gatcagagca ggcaatgata ctgaattatt agaactatta aagaaagcgg ttttttaata
     4141 taaggaggaa atcataatga aacgaatttt agtagcatgc ggaaatggta tcgccacttc
     4201 aacagtagta gcaacaaaag ttagagagta tttgcaacaa catggcattg atgtttcaac
     4261 gactcaaaca aaattgatgg aagttccagg aaaagttcaa gactatgatc tattggtgac
     4321 gactggtcaa tttgatggtc aaacaggtgg tgtaccagta atcaaaggga tgccgatttt
     4381 aacaggtatc ggtgccgatc agacaatgga agagattttg aacttactga aataatagct
     4441 gatcaaagga gctgaaaaaa tgaaaatcat tatgaacgtc tttaacttct ttattgatgc
     4501 tggaccaaca gttatgttgc cagtcatcat cacgattatt ggtttgattt ttgggttaaa
     4561 gatttctcgt gcttttaaat caggtttgac attaggtatc ggttttgccg ggatcaagtt
     4621 gatattggac tttatgacga ccaatgttgg accagctgcc aaggcgatgg ttgatcgaac
     4681 tggtgttaaa ctagatgctt tggatgttgg ctggggttcg attgcggctg ttacttgggc
     4741 atcgccaatc attccaatct tgatttttgc cattctattg gtcaatatcg ttttgctgat
     4801 tttgaaacgt acacatacgc tggatgttga tatctggaac tatcaccata tggcaatcgt
     4861 tggtgttata gtgtattttg tgactaagaa tgtcttctta ggtgtaggtg catcagtagt
     4921 gatggcaatt gcgactttta agatttcaga ctggtcgcaa ccgatggtag aaagtttctt
     4981 cggaatccct ggtgtttctt taccaactgt ttctgcgctt tcatcactgg ttattgcttg
     5041 gccgttaaat tggctgctgg atcgtatccc gttattcaga aaatcaaaat tcaccatcaa
     5101 agatgcacaa aaatatttgg gattttttgg tgattctatg atcatggggt tagttattgg
     5161 gatggtgatt ggtgttttag ccggctatga tatcaaagcg gttttacaat taggtgtcag
     5221 catgtcagct gttttggttt tgattcctaa gatgacttca ttatttatgg aaggattgat
     5281 gccaatttca gatgctgcac aaaaatggtc acaaaaaaaa ttcaaaggta cacgtttatt
     5341 tatcggattg gatgctgcag ttgttgtagg taatccagat gttatcacaa cagcattgat
     5401 cattattcca ctaacgattg cattagcttt agtattgcca ggaaaccgtg tgttaccatt
     5461 cgcagattta gcggttgttc ctttccgcgt ggcaatggtc gttgccttga ctcgtggtaa
     5521 tttattaaaa aatatcatta ttggcttagt tgtgacagct tcattgctat ggtgtgggtc
     5581 agctactgcg ccaatgctga cagcaattgc caaatcggtt ggtattaact taggaacttc
     5641 ttcaatgtta atctcatcat ttgctgcgac ttccatgatc caaagttatt tggtatttat
     5701 cgcatttgct tacaaaccaa tcatcggtat tccagtgttg ttattagcct ttgcgggcgc
     5761 atggtattac ttcgatggta tcaaaaaagt taatcatgaa atggatgaac cattgaaggc
     5821 acaatagtag gagggtttaa attgaaaatt tcagatgtca cagtgtataa agtaaaacca
     5881 agatggattt ttgttaagat ctcgactgac gaaggaatcg aaggctgggg agaaatgatc
     5941 tcaggaacta aaacagaaac ggttgttgct ggagcttatg agataggaaa ccggctgatt
     6001 ggacgtaatc cgtttgaaat cgagcgcctc ttccaagaaa tgcatcgatc tttcttccga
     6061 ggcggtccta ttaatggaac catcgtttcg ggtttagaaa tggctctgtg ggatatcaaa
     6121 ggaaaagcgt ttaatgttcc ggtttatgaa ttattgggcg gtgcagcacg tgataaaatc
     6181 aaggtttatt cttggattgg tggagatcga ccaagtgaag ttgcagaaca agcacaagat
     6241 cggtttgatc ggggctttac ggccgtcaag atgaacgcga cagaagagct gcattatatc
     6301 gattcctacc aaaaagttga tgaagtggtg gaacgagttg cgtctattcg cgaacgtttt
     6361 ggtgatgcaa tggatattgg tgttgatttt catggacggg tccataaacc gatggcaaaa
     6421 gtgctagcaa aagctttgga gccattccat ccaatgtttt tggaagaagt cgttttacct
     6481 gaaaatgaag aacactttaa agaagtagct gattctgtgg ctgtaccatt agctactggt
     6541 gaacgcttgt acactcgttg gcaattcaag aacctcttta aacaaggtac tgttgatatt
     6601 atccaaccag atgtggctct ttgtggggga atcttggaaa cccgtaaaat cgcagcgatg
     6661 gcggaagctt acgacatggc tgttgcgcct catgcgccat atggtcctgt tgcactagca
     6721 gcaactctgc aagtagactc atgtacgcca aacgtcttta tccaagaaca aagcttaggg
     6781 atccactata acaaaggttt tgatctgtta gattttgtga agaacaaaga agttttccaa
     6841 tataaagacg gctacgtaga tctaccttca aaaccaggtc tgggcttgga aatggatgaa
     6901 gacaaaatca aagaaattgc acaagaaggt ttgatttgga ccaatccgca atggaaaaat
     6961 tatgacggta cgatcgctga gtggtgataa atgaaaataa aggatgatga aagatggcag
     7021 aagcacttat gatcacagtt tttttaggac tgctgattat tttgcaaata atcgatcaaa
     7081 agaaaattat cgtgtttgct cagagtagtc ctgtaaggaa gagtttgagt attttactga
     7141 cagtcgccat cctgttgatt ttctggccgg aacatctgac tgatcagatt aaactggttg
     7201 cttttgcttc tgtcattttg atcaatggtt ttttgaagga gggtctagca aaagattctg
     7261 ttgttagatt tggagtattg aacggtaact ttaatcgata tcctcaagtc caagtagaaa
     7321 aactaggtga cgataaatca tttatttcat tttacaaaag caagaataat tctttttcaa
     7381 tgatttttga taaagatcaa actgaaatga tggatgtctt tcaagaactt gatatccatt
     7441 ccaaaattac tttagatcaa agttcggaag aaaaaagagc agttgtaaaa aaagataatg
     7501 aatgattcta ctaccgaaat tatgatttct ttatcacgaa taatgaaact tgttttaatg
     7561 aatcatattg attgaagtat acaatgtata ttaagttttg gaaagaaata tattggtaca
     7621 agatatagca ggaatcgatt actcgatccc tgcttttttt aacttccttt gcagcaaaaa
     7681 aagctcgtaa tctaagagat gatggatagt ttttagaata tatatatttt cttcaattcc
     7741 agaaaaaaat gaccaagcta gtatgctaaa aaagtacgat gcttctatga tcttttttcc
     7801 gtctttatta acagatgttt taattgatca acgcatattg taatctaccc taatttccat
     7861 agttcttcac cacttttaaa aatagtaaaa cccctacaag tatgctatag taaattgcta
     7921 aagaaaaacc aataaggtgg gagatgtgtg cgcgatcaaa caagcggaaa aatgattcgt
     7981 ttttattata caagtttgca ttttctttat tggatgatgt tttgttctgt ctatgggttt
     8041 gcgacattgt ttttgattta tttcaagatt gatccagcta aaatcggagg gatcttagca
     8101 ttggtcaata ttgtttcaac ggtgcttcag ccttttgtca gtcaagtgat catcaagaaa
     8161 agaaagatcc cactgatcac cgtgctgaag acgatgatag caggattagg agtgattctc
     8221 ttagctgggg tattgattcc gaatttgccg atcattactt atatcttggg tgggatttta
     8281 gtagtttcta tgcaatcatt tctcaatgcg ctggcatttg agtattcaaa taccggctac
     8341 aggatcaact ttggcttttc aagagctggt ggttcatttt cttatgctgt aacttctttt
     8401 ttactgggac agctgttagc gatttattct gccgcgattt tgccaatctt ggttatagtt
     8461 gaagcagtga tatttttcgt tattttgcat ctgctgccgg atgtaaaaaa tcaagaagtt
     8521 tttcctatga caaaagaaca ggatcgctca gtttctttga gaaaaaaata tcctttcctg
     8581 attttgttat ttttaggttt cagttttctt tttactttcc acaccatgat caacagcttt
     8641 ttggcacaga tctttgaaaa tatccacggc ggcagtaaag aagttggcat cgcattgatg
     8701 atcgccgctt tatgcgaatt accgggaatg atcttttttg aacagcttgt caaaaagaaa
     8761 tccagcaaat tttggctgtc tgttgccagt atttcatttg ctgtcagagg attcatgatg
     8821 ctgctggcca gttcgatcct gatgatggaa atgacgcatc tcctacaagc cttcagtttc
     8881 gctttgttta ttccagcttc ggcttatttg ttcaatcaat ttctggcgga tgatgatcgt
     8941 gtatttggac aaacgatgat catcatcgcc acaactcttg gtggggtgat aggaaatgtc
     9001 ttgggcggca gtctgctgca gcatttcggt gtatgggaga tgctgatttt cggtactggt
     9061 tttgcggtgc taggtgcttt attaactatt ttgggcattc gaaaaatacc gaagacagga
     9121 aacgatagtg attctcttgt tgagtagcat aaaaatactt agatactagt tttatataaa
     9181 taacatatag tgattattgg cgtaattaca aaaaacgaga ataacttaag taagcatttc
     9241 taaataattg agaaacctca tctaaaaaga taagaaaata tccctaataa aacttctgta
     9301 acattttaga taactgattg taatctgaat aataaacta
//
LOCUS       DANJMA010000076         9293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000076 DANJMA010000000
VERSION     DANJMA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..183
                     /locus_tag="PWU31_002426"
     CDS             <1..183
                     /locus_tag="PWU31_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084904.1"
                     /translation="QHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKR
                     KNEIYYEQQLSQKERV"
     gene            244..1704
                     /locus_tag="PWU31_002427"
     CDS             244..1704
                     /locus_tag="PWU31_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL1084905.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1715..3919
                     /gene="topB"
                     /locus_tag="PWU31_002428"
     CDS             1715..3919
                     /gene="topB"
                     /locus_tag="PWU31_002428"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1084906.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3935..4084
                     /locus_tag="PWU31_002429"
     CDS             3935..4084
                     /locus_tag="PWU31_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084907.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4160..4753
                     /locus_tag="PWU31_002430"
     CDS             4160..4753
                     /locus_tag="PWU31_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL1084908.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4766..5665
                     /locus_tag="PWU31_002431"
     CDS             4766..5665
                     /locus_tag="PWU31_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL1084909.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            5668..6153
                     /gene="ssb"
                     /locus_tag="PWU31_002432"
     CDS             5668..6153
                     /gene="ssb"
                     /locus_tag="PWU31_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1084910.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(6294..6497)
                     /locus_tag="PWU31_002433"
     CDS             complement(6294..6497)
                     /locus_tag="PWU31_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL1084911.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            6572..6832
                     /locus_tag="PWU31_002434"
     CDS             6572..6832
                     /locus_tag="PWU31_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084912.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            7269..7475
                     /locus_tag="PWU31_002435"
     CDS             7269..7475
                     /locus_tag="PWU31_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL1084913.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            7475..7726
                     /locus_tag="PWU31_002436"
     CDS             7475..7726
                     /locus_tag="PWU31_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084914.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            7741..8178
                     /locus_tag="PWU31_002437"
     CDS             7741..8178
                     /locus_tag="PWU31_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084915.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            8171..8878
                     /locus_tag="PWU31_002438"
     CDS             8171..8878
                     /locus_tag="PWU31_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084916.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
BASE COUNT         3427 a         1587 c         1908 g         2371 t
ORIGIN      
        1 cagcataatc aagtgatttt aaaaggaaaa agttatggaa aagagattca aacggcgtta
       61 ccttcagaag aatttgcgct aggaaatcca ttccgatttt cacttgaaac agtgtcagaa
      121 gtggccaaac gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta
      181 taaaacaagt aaaaaagact atctgaaaaa cggatagtct tttttctttt gggaggtttg
      241 aaaatgaccg aaaaaaaaat gagtcttatt gatcgatgta aacagatcga tatagtggat
      301 tttgctcgaa acaatggaat ggctgtagtc aataaaggac gagactatcg cttagaagac
      361 catgattcat ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt
      421 agtggcgaca ttattgaatt agctaaactt tttttcatag acaaagagat ccaagactct
      481 aagcaacaat ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga
      541 gtggaaaatc ttcattttga aacagaaaag tacaaagatc accccgttga ttatcaacca
      601 ctcacagaaa aaggaaggaa ttatctgaag gaagaacgaa aactaccaga gtggctgatt
      661 gattatgcag aaaaagaagg gttgattgcc gaactaaagc caaaatatga acggcaaaat
      721 ttcttagttc gagacgatcg tttagatcat gcggtcgcct ttttatggaa agatccacag
      781 acaaaagaga cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt
      841 gagagaggca catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta
      901 aagatcggtg accctaagca gttaaaattt tttgagagta gtattgattt acttagctat
      961 gctgcactga atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa
     1021 caccatgtga tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc
     1081 tttcctcaat ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa
     1141 aaagaacaat taatgggcgc agtcgatcca tttacgaatc agaaagtacg ttgtgaacga
     1201 ggaatcgcta atgattggca agtgccaaaa gaatacaaaa tcatttatga agaagtcgca
     1261 aaagaagaaa aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg
     1321 caattaacga accaactggt ttccgctcac aaagtaaatg cgttttttgg ccagcagctt
     1381 tctgttaacg attcaataga agccattaat ttgaaagata tttgtagaga agtcgcaaaa
     1441 gaattaaaag tttgtgaacg cgtggatggt acgtacgatt ttgatcgttt ttatcaagaa
     1501 aaaggggata ttaatgcgca gattcttttc tcttataaag cagaacaata ctacaaaggc
     1561 tataaaaatc atgaacatga atttatacct gaagtaaaaa aggactggaa cgatcaattg
     1621 aagcatgaga tccagcaaca agaaatcaga aagcaaaaac gtgccatgtt gtttcaacaa
     1681 ggcagacaac aagaaaggga gtgaaatcgt catgatgaaa accgttattt tagcagaaaa
     1741 gccgtcacaa gcgaaagctt atgccgatag cttctctaaa gccactcgaa aagacgggta
     1801 ttttgaaatc caagatcgat tgtttcctgg tgaaactgtg atcacgtatg gttttggtca
     1861 tttagtcgaa ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga
     1921 acatttgcct attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca
     1981 attcaaggtg gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag
     2041 cgatcgtgaa ggggaactga tcgcttggac gatcattcaa caagtaggcg ccgatcaagg
     2101 aaaaaccttt aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt
     2161 ccagcagtta cgagacgcaa aagagaccta tccaaaattt gaagaagcac aagcacgtca
     2221 gatcgccgat tggttaattg ggatgaacgg tagcccactg tatagtttac tcttgcagca
     2281 aaaaggcata ccaggaagct tttccttagg cagagtccaa accccaacgc tttacatgat
     2341 ttatcaattg caggaaaaga ttagaaattt caagaaagaa ccttattttg aaggaaaatc
     2401 cgaagtaatc gctcaaaatg gcgcatttga tgcgaagctt gatccgaatg aaacccaagc
     2461 gacgcaagaa gcatttgaag attatttaaa agaaaagggg gttcaattgg gaaaacagcc
     2521 aggcatgatt cgccaagttg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc
     2581 tctatccagc cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagacacttt
     2641 ggaagccatg caaggcttgt atgaagggaa atatctaagc tatccacgaa cagacacccc
     2701 ttatattacg gaaggggagt atgcgtattt gttggatcat ttagatgaat acaagcattt
     2761 tttaaaggcc gaatcaatcc caacccctat tcatacgccg aatagtcggt atgtgaataa
     2821 caagaaagtc caggaacatt acgcgatcat tcctacaaaa acggttatga cagcagcggc
     2881 ttttgaacaa ctaagtccac tgcagcaggc catttatgaa caagtgttaa aaacaacggt
     2941 ggccatgttt gcggaaaaat atacctatga agaaacaacg atcttgacgc aagtccagca
     3001 gcttcagtta aaggcaatcg gaaaagtacc gatagatcta ggttggaaga agttatttgg
     3061 caaagagaac gacggaaaag agaaggaaga agagccgcta cttcctaaag tgacaaaagg
     3121 agaaatggtg acggtagatc tacaagtcct tgaaaaagaa accaagccac cacagcctta
     3181 taccgaaggt acgttgatca cggcgatgaa aacagctggg aaaaccgttg atagtgaaga
     3241 agcgcaatcc atattgaaag aagtggaagg aatcggtaca gaagcgacac gggcgaatat
     3301 tattgaaacc ttgaaacaaa aggaatatat caaggtagag aaaaataagc tcgtggtgac
     3361 gaataaaggg attttgcttt gccaagccgt ggaaaaagag ccgctgctta cgagtgccga
     3421 aatgaccgcc aaatgggaaa gctacttgtt aaagatcggt gaacgaaaag gaacacaaac
     3481 cacttttcta gccaatatcc aaaaatttgt ctctcactta ttggaagtcg taccagggca
     3541 aatccaatcc actgattttg gatcgacgtt acaagaagtg aaagcagcca gtgaaaaaca
     3601 ggaagcaacg cgtcatttag gtgtttgtcc aaaatgtcgg gaacaagaag tcttgcttta
     3661 tcaaaatgtg gcggcatgta cgtctgaagc ctgtgatttt aaattatgga caacgattgc
     3721 caaaaagaaa ctcacagcca cacaactgaa agagatcatt caaaacggcc gaacaagcca
     3781 atcagtcaga ggactaaaag gtcagaaagg ttcgtttgaa gcaacgatcg ttttaaaaga
     3841 agactttaca acaggctttg aattttctga aaagaaaaaa acgaactata agaaaagaac
     3901 acgccgcact acaaaataat gaaggggtta tcctatgcga aatacaacac acaaacaaca
     3961 agaaatcgaa aatcaaaaag caaaaattcg tttagaacaa gagaaattac gtgcaaataa
     4021 aagaagactg caacaattaa agaaccagca gcgcattcaa aaagaaatcg aattagatcg
     4081 atagacgtta atcttgttac aaataaacta ctatgatata tttgatgtag tgtatttgta
     4141 acgaagggag gggaagtaaa tgcaagataa actgaaagaa ttgttggcta ttcgcgatgg
     4201 aaatttatct atgaaggaag ccaggaaatt tggaatcgca gcaaccactg tacaaagatt
     4261 ggtgaatcaa ggaaaattga tcaaagttga tcgtggttat tatgtagagg atggtcatgg
     4321 aatagatgat ttattttggc ttcagcaacg attttccagg ggaatcttct ctcatgaaac
     4381 gactttagat atttaccagc tttctacgaa tatgccgaaa ttcattcact taacttttcc
     4441 ccatggctat aacgttgatc gttcaattac aaaagagcag cagttacaat ttcatttcgt
     4501 gaagaaagaa gtttatgaac taggaaaagt agagggaact tcctttcaag gaaatcctat
     4561 tactatgtat gataaagagc gtacactttg tgatatttgg aatccgagat atgaatttga
     4621 atatgaaatg aaattagaag cattaaaaga gtacatggaa gacccattga gagatccaag
     4681 taaattaaga gattacatga caaaactaca tgtagaaaaa gaaatgaaat accacatgca
     4741 aagcttatat tgatggagga aaaagatgac accgacacaa tttgcagcaa aaacaagaaa
     4801 tttagcaaag agtacaggat taaatgcaca gttagtgcaa cggcatttta tgatggatcg
     4861 tctaattgaa caaatttctg aatcaaagta caaaaatgac ttcattttga aaggcggctt
     4921 tttattagga tcaaaatatg gaatcgatag aaggacaaca attgatatcg atacgacatt
     4981 tagaaatagc aaattaactg aagaaaaatt gaagagcatt tttaatgatt taactagaaa
     5041 acccacaaaa gaaggcattg ttttctctat tcaaggaatg aaggaaacaa gagaagccga
     5101 tttttatcct ggatttcaaa ttaaattggt ggctaattta gagaaagtac gtgtaccgtt
     5161 taaaatggac gtgacaacag gagattctat ttatcctgaa gtagatacac actatcacca
     5221 attaatgttt gaagataaaa aagtggagat tccagcttat ccaacagaac aaattattgc
     5281 agaaaagcta agtgctacgt tcagctttgg cacagataat tcgagagcaa aagattacta
     5341 cgacttattt accattccga aactagaaaa tatcgatacg aaagagttat acaaaagtgt
     5401 tcgaaataca tttacaaaac gtggggatac aaaaccgttg tccttttatt atgaaagaga
     5461 aatggataag atcagaggaa atgactattt ggaaaagcag tgggaaaaat atcgctcagt
     5521 taacacgttt gcgaattcca tttcatttac agatacagtc aacgaaattg atcacctaat
     5581 gaaaagtgtg atcaaaattg aaaatcaaga aagaaaacgg cagtttaaaa gatcacaaca
     5641 aatgaatcaa gaaatggaga gataaagatg atcaataatg taactttagt aggacgctta
     5701 accaaagatc cagatttacg ttataccgcg agtggtactg cagtagcgac ttttacccta
     5761 gctgtcaacc gaaatttcac gaatcaaaac ggcaatcgag aagctgattt tattaactgt
     5821 gtgatttggc gcaaaccagc agaaacgatg gcgacattag ctaaaaaggg cagtttgatc
     5881 ggcgttgttg gacggattca aacccgaacc tacgataacc aacaaggaca acgtgtgtat
     5941 gtgacagaag ttgtggccga taatttccaa ctattagaaa gtaaagcagc gacagaaagc
     6001 cgtgcgcacg ctgatcaatc gtccagcagt ccaagcccta ccacctttga acaacgggat
     6061 gtagctacgc ctaacaacaa tggattgaac gcttcacaaa atccatttgg cggacaatca
     6121 atcgatatta gtgatgatga tttaccattc tgattgtaaa agatcaataa agttgttata
     6181 atggatacga aaagaggagt tgcgctaaca actcccctaa cagcaccgtt taagacggtg
     6241 accagcttaa tattattttt gaaaaataat cgtcgaccgg ctaaagctga cggttatttt
     6301 ttttgattgt tgtttacaac tgctaagatt ccaaaaatga ttgttgcgat caaagaaccg
     6361 aaactaatca ttaatcctag cgccgtttca acaggcaaaa ggcttctcct ttctgtaaga
     6421 ttttggaagt acgcacatac gcaccacctc actttcaatc ggatagccac cgtcacaaac
     6481 tttactgtct tgatcattat agcatatcta aaatcgaata aaaattctca attttagctg
     6541 tttttgctat aattttagcg aggtgatcgt gatgaacaaa tatgttggtt tgcttgataa
     6601 aatccgagta ataaaaacac aaccattatt ggttcgcttt actttacaaa caattcatga
     6661 atcgattaat tgtgtagttg cagatattga aattatcgat aaattgttaa ttatggatga
     6721 tggaaaatac aatattgccg tcacaggtca tttcaataaa cggaatcaat tagtcattga
     6781 atcgatgcaa attagaaacc ccgatcattt tacgcgatcg atgggcatat aaaagaagag
     6841 ctgcagtcct aaatgaggat tgcagctctt tttgttttac atagaaacag gaaaagacct
     6901 ttttaggaag acacaagtgc cttctcaatt gaaaaacctt tctctcactc caaccgccac
     6961 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagatatgg tgtcttgcaa
     7021 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgctatctc
     7081 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     7141 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     7201 aacaaatcaa taaagaaaga actaacgaaa ggaaataaaa cctttcctaa gaggaggcag
     7261 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     7321 actatcatgc accacatact gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa
     7381 gctatcgttt gtatcaatgt ccaacatgcg ggaaaaaata taccttgtct tatggcgatt
     7441 atgttttgat tgatgagaaa gtaaagaaac ggtgatgaaa acacaacaca aattattgac
     7501 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     7561 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     7621 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     7681 ggaaacttat gaaatcgaat attggttaac caaattagag aactaaaaag gagaaaaaat
     7741 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     7801 atggaggata tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca ttacaaggaa
     7861 aaattgtcag tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
     7921 acgtactatc gaacactgga agaactgaaa aaacaaaatg gaaaatggtt tttccaagcc
     7981 ttactatgct tggaagtagg tgtcaaacca agcactatca aaccttccga atatcaagcc
     8041 ttagaactga cttatgcaaa attcgcacag attaagaaag ctaaagcaat ggctagcgac
     8101 tggctagata cttttgaaaa tatcgaaaaa aatggtgcat tttatacgat tgaatcggag
     8161 gagaaagata atgaataagg tggtttatca gctaacaaca gaagatgttt cgcaattttt
     8221 aacaaacaaa gaacacttga atgaagtaat cagacggatt caaaaagact ttagttgtat
     8281 ggaagaaatg aagtacttta ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac
     8341 gagtcaaacg aaggttacag tcagccataa tcgtttagac gtgtataaac gtcgtcctga
     8401 cacattaaaa aaaggagcgg agcttctacg actagaaaca gaaaaatata tcatgaatgt
     8461 ttacctagaa aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca
     8521 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
     8581 aatggaagag ctaaacagca aagatgcgac ggtagacgat tattaccttg tatttactag
     8641 atttttgacg tgtgggacgg aatctatcgt gttaatggga atggattatg aactcactgt
     8701 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
     8761 cacgacttta actgattttt tagacaccta cacttacgac gatacagaag aaatccaaaa
     8821 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaag gtcaataacc
     8881 atttataccg aaaagtgagg atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa
     8941 tggttcttat ttcgcaccaa tcgatccaac cgcccccgcc gcttaggtgg tgcgaaataa
     9001 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
     9061 ctattgttcg tactgtttgt tggcgagata cttatgcgtc atggtgcagc tctacattta
     9121 aacttcgtag tattcttagg aatactggta ttttgtttga tcttttggcg ctttgtgtta
     9181 acccttgtga gtctaatatt caagttgatt gcagccatat ttttaatagg gttatttcga
     9241 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgt
//
LOCUS       DANJMA010000077         9211 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000077 DANJMA010000000
VERSION     DANJMA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            140..385
                     /locus_tag="PWU31_002439"
     CDS             140..385
                     /locus_tag="PWU31_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084917.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(569..1318)
                     /locus_tag="PWU31_002440"
     CDS             complement(569..1318)
                     /locus_tag="PWU31_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1084918.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1504..2208
                     /locus_tag="PWU31_002441"
     CDS             1504..2208
                     /locus_tag="PWU31_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL1084919.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2248..2805
                     /locus_tag="PWU31_002442"
     CDS             2248..2805
                     /locus_tag="PWU31_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1084920.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2813..3952
                     /locus_tag="PWU31_002443"
     CDS             2813..3952
                     /locus_tag="PWU31_002443"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL1084921.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3964..4395
                     /locus_tag="PWU31_002444"
     CDS             3964..4395
                     /locus_tag="PWU31_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084922.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4409..5854
                     /locus_tag="PWU31_002445"
     CDS             4409..5854
                     /locus_tag="PWU31_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDL1084923.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            6140..7765
                     /locus_tag="PWU31_002446"
     CDS             6140..7765
                     /locus_tag="PWU31_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1084924.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            8397..8750
                     /locus_tag="PWU31_002447"
     CDS             8397..8750
                     /locus_tag="PWU31_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084925.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         3189 a         1289 c         1793 g         2940 t
ORIGIN      
        1 gtcaaaaagt aaataatatc agttatcaaa tttcctgtaa attttcttga aactacagca
       61 tattattact tcaaaacaat ctacattttt tataaaaaag gaaatgaata actgaaaaaa
      121 ttattatagg agagaaaaaa tgtcaaaatc taaaaaaaat atcaccataa ttggtttagg
      181 cttaaccgta tatttattta cagaatttgt ttatagacat gtgaatttat ttgcagaacc
      241 tttatttgta ttatacgaat caaattcctc aatcgtcagt gaacttgcta atttaatgat
      301 agcattgctg tttactcccg cgttgagagt cataactatt ttggtctgtt tagtgctatt
      361 gttttttgtt aataaaaaga aataacgtag tatttcaaaa gacaaagaag tgggatgatg
      421 attgctatag tatgttatac gtggtgctag gtaagtgaga ataaacaaaa aaggaaacac
      481 gcaataaagt ttatttcaaa actttattgc gtgtttcttt tttagatttt ttatatctgt
      541 tgctttagta gttaccaata gttttcgctt aatcaatcaa aacgattttt tgtttattgt
      601 attctttacg aaccttttca ggaatttctt ctgaaataag ataggtaagg tcttcaattg
      661 gaccgacgat atgattactg atggtgccga atttatcatt agttatcatc ccaagtactt
      721 cagtagaaat acgtatcatc tcacgtttaa tgtcggattc ttgcatagtt ggcacgctcg
      781 ttccaacatc tagatctatg ttataaatac ccataatata aaggtcggct cgaatgtttt
      841 gtagttgctt ataagcttgt accccaatat tgatcataga ttgtttataa aaagtaccgc
      901 caatattgat aatttccaaa ttaggatgct gtgctaatgc tatggcaatc ggcgggctat
      961 tggtaatgat tgttgcacga aaatcttgtg ggatttcttt tgctaaagcc aagttagttg
     1021 tacttccatc aatgatgatg acagattctt cttttaaaaa agggagggct ttatgagcta
     1081 atgcagtttt ttgttcgcta gctacagttt ctcggtagtt aaaattggta attggcggac
     1141 caacacgtaa agcgccgctg tgtactcttt ttaagagacc ttggctatcc aaatctttca
     1201 aatctcgtcg aatcgtgtct tcggatacat tgaaacgttg acttaaatca ctagcgataa
     1261 ctttttgttc tgcgtttaat aaatctagaa ttttttgatg acgttctaat ttcaacatgt
     1321 tgcgtgctcc ttcttattta ttcttgttta tcatgtcatt tatgcgtttt tatgtcaata
     1381 atttccgttt ttaatacact aaaaaggcat aaacaagcaa aaacaaaata tttttatgca
     1441 gttttatgtt gactagtatt tgttaagcgg ttacaatgag ggggtaatta aaggagggaa
     1501 tgtatggaga aaaaaattat tttgtcgcct tcacttatgt gtggggatct agcaaatttg
     1561 gaaggaagca tccaagaact agaaagtatt gggatggatt ccttgcatat tgacgtgatt
     1621 gacggttctt ttagtcccag tatgccttta gggatcgaaa caatcaaaag aattagagaa
     1681 atcacttcaa tgaattttga tgtacacatc atggctaagg ataacgataa cgaatttttt
     1741 attggagaaa tgttgaaaat tggcgtagag agcattactt ttcattatga aacaagcctc
     1801 catattgatc gatatatcca gctcatcaag aattctggaa caaaagtagg gatagctttg
     1861 aatccagcaa ctccgttaaa tgtattacaa tatattttac ccgagattga taggatttgt
     1921 ttgatgctga ttaatccagg ttttgcaaca aacaagtcag aaaaacaagt ttcttacgcg
     1981 ctaaaaaaaa ttacagatct ttgccagtta gtaaatgaga aagagttagc agtagatatc
     2041 caagtggatg gacgggtatc gttagaaacg atcccgggat tattagatgc aggcgcaaca
     2101 aatctggtat taggaagtac aagtttgttt cttaaaggaa atacattagc agaaaatcaa
     2161 aaactacttt atcaagcgat gaagcaatca gttaggaaaa atcaatagat gtagtggtcc
     2221 tgaataatag aaatggaggg gaaatagttg agttataagg cggcgaaaca aaatattgta
     2281 agagaaatta ctgaaacatt agaccgtatt tcttcaaaag atgtggaaca attgataaag
     2341 aaaattgaaa aagcggaacg agtttttttc gtgggggttg gcagagtact gttagcgcta
     2401 gaaggaatgg caaaacgctt agggcatcta ggaattcaga cagtagtcgt aggacaaatc
     2461 actgaaccgg caattacaga taaagattta ttgattgttg gttctggtag tggggaaagt
     2521 gctttcccac taactattgc aaaaaaagca aaggaatttg ggtcccaagt tgcccatata
     2581 ggatcaaacc cagacagcac aatgaaagaa tattcagatc tttttattcg tatacccgta
     2641 caaacaaaat tagcgttacc agaggaaatt tcttctatac agccaatgac tagcctgttt
     2701 gaacaaagtg ttcttttgtt aggagatagt attgctttag tgatgataga agaaaaagca
     2761 attgatatgt caaagttgtg gcaataccat gctaacttag agtaggtgaa aaatgaaagc
     2821 ggtacatttt ggagcaggga acatcggcag agggcttatt ggacaaattc tacatcagaa
     2881 tggattcgat ctatgtttta ttgatacgaa tcctgaactg atccatcaat taaatcaaga
     2941 taagggttat actatcgaaa ttattgatga aatgaacagg agtgatttca ttgatcatgt
     3001 tgtggcactg aatagtttat ccgagctaga agaggtgatt acagcaacaa aaaatgcaga
     3061 tatcttaaca acatctgttg gcgcaataaa tttagtaaaa attgcaccga tcattgcgca
     3121 agccttaagt aagcgatttg aacaaaagca tagcatcaat atcgtagcta atgaaaatgt
     3181 gataaatgca agtgatgtgt tgaaaaaaga aatttattct ttgctgtcag aaaaagaaca
     3241 agagaatttt gatgaatatg catattttgt gaatacggca atcgatcgac aatctttagg
     3301 aaaagttctt ggaggaaaaa tgatctctgt tgtcgaacct tattatgaat ggataatgga
     3361 tcgacagcag ttagatccaa ctacatcttt tgatatgaaa aatgtaaatt tggttgatga
     3421 tatggagcct tacattgagc gaaagcttta tatcgttaat gctgctcatg cggcagtagc
     3481 ttatttaggc gcactgaaag gatataagac gattcaagaa gcaatccaaa atatggagat
     3541 tttcaatcta gtcaagcaat ttttaatgga gaatatagag tactttgtac aaaaatatca
     3601 tttttctaga gaagaattaa catcttttgt ggaaaaaaca ttaaaccgtc atgggaatca
     3661 gaaactctcc gatgaaatta ctcgtgtagg aagttcccct attcgaaaat taggccctaa
     3721 tgatcgttta attgctccca tagtaaaact agattcatat catttacctt acgatgcagg
     3781 aataaaagta attgctgcgg gatatcatta tcggaatctt gctgatccag aagctgaaca
     3841 actacgtcga atgattgtaa atgatgggtt gaaagcaacg attaaaaaga tttctcgttt
     3901 ggaaggtaag ctgctgaatg aagttgtcgc tgcttatgag tcccagattt agaggaggag
     3961 aatatgctaa aagaacaatt gtcaggaaat acaatgttta tggatgcgat cgattcatgg
     4021 caagaggcaa tccgagtaag tgcgcgtcct ttgataggaa agggaataat agaggaaagc
     4081 tatgtacaag cgatgataca aaatgtgttg gataacggaa attatattat attactgcca
     4141 ctagtagcaa tgcctcatgc gcgtccagaa tttgggagca aaggggtcgg gatgagcttt
     4201 ttatgtttag ataagccagt catgttccca agtgaagaac cagtaaaatt cttctttacc
     4261 ttttcttctg attcgccaaa gggacattta gacttaatgg ccaatcttgg agaattgctg
     4321 agtgatgaaa aaatgtatca aaaattattc gatgtgaaga cagaaaaagc attgttagat
     4381 ttgataaaga agtaaaggag agaagaaaat ggaggtagca aacaaaaaaa aacgagtctc
     4441 catgcgagct aaagttcaag cgattggcgg tttcttaaca aatatggtat tgccaaatat
     4501 aggcgcattt attgcatggg gaattgttac tgcattattc attccaactg ggtggttccc
     4561 aaatgaatct ctgaatgaac tcgtaagtcc gacagtcagc tatctattgc cgttactact
     4621 tgcctatact ggtggtcgga tggttggtgg tacacgtgga gctgttttag gtacaatcgg
     4681 aacaataggt ttgattattg gtgctgacat accaatgttc atgggagcaa tgattatggg
     4741 gccacttggt gggtggatga tgaaaaaaac agatgatttt ttagatggga aagttcctgc
     4801 tggttttgaa atggtcgtca ataatttttc tataggaatt attggtttca tactgatgtg
     4861 cctttcttat ctggtagttg gaccaataat ccaatcagct aatcaatttg taacgagtgc
     4921 aattgaagca ttagtttcaa ctggtttttt accgatactg tccttaatca acgaaccggc
     4981 aaaagtctta tttttaaata atgttattga ccaaggtgtt tattatccat taggtatgca
     5041 acaaacatta gaggcaggca gatcaattta tttcatggta gcatcaaatc ctggccctgg
     5101 cttaggacta ttgttagctt attctttatt tggtaaaaag gcagcaaaac agacagcacc
     5161 aggtgcaatc atcatccatt ttttcggcgg aatacatgag atttattttc catatgtatt
     5221 aatgaaacca ctcactatat tatctatgat tgctggtgga atgacaggaa tcatcacttt
     5281 tgaattattt gatgcgggcc tggtagcagg tccaagtcca ggttctattt tttcttattt
     5341 agctctaacg cctcggggaa attttttggg tgtcatggcg ggagtggtca cagcaacact
     5401 ggtttccttt tttgtaacat cagtaatttt aaaagcagat aaatcaacag atgaagacag
     5461 aaattttgaa ctatcagttc aaaaatcgaa aacgatgaaa tcagaaggaa gaaaattatt
     5521 aaaagaagaa aatgctagag aaatatgtac cgagaaaaaa gagatgaata aaatttcctt
     5581 tgcttgtgat gcaggaatgg gaagcagcgc aatgggcgca acaatattca agaaaaaatt
     5641 gcaaaaagca ggtattactg atgttgagat aaaaaattat cgaattgaag aggtaccaga
     5701 agatagtgat ttggtgatcg tacatcaaga tttggaggaa cggactagaa aagcacatcc
     5761 taacatgaaa attgtgacat taaagaatta tttgcaagat ccagtaatca atggattagc
     5821 agaagaaatc attggattaa aaacagaaga ctaatttatg ggaatttttg attaaccttt
     5881 attaaaatta ttttgcctaa tataaaaagg ttgtgacatg gcttgtgtcg caaccttttt
     5941 ttgtcgcaca aataattaat cggatatttt atgttcatat tttttgtgat caacgagaac
     6001 tgatggggca aagatacatt ttagatactt ttaactgtta atttacaaaa caaggtgaaa
     6061 cgaatagaaa aatgaaaaaa tcgatacgga agaatatgtt tgattaaaac atgcatgaag
     6121 agaaggaggt tgatgataca tgaaaaaaat atattcaaag gaaatcgatt gttttagagg
     6181 cattgctgtt ttagcagtga tattttatca tttgccaaat agtttgctgc caggcggatt
     6241 gcaaggagta acgatctttt ttgtggtttc tggtttttta atggctaaaa atacgataag
     6301 agatgaaaaa aacagacaat tcagtttttt acatttttac tataagcgta tcaaacgtat
     6361 ttatccattt ttactgtttt ctttggtcgt attcattttc atactaggga ttagcaacat
     6421 tcgattgata ggaaacattc gtagtgagct acccagtttg atttttggct ataacaattg
     6481 gtggcagctg aatcaaggag tatcttattt tgactcttac tttaatgctt ctttatttaa
     6541 acattattgg tcgctttcag ttgaactaca attctatttg atatggccat ttttgtttat
     6601 tgtcattaag cgtatgagaa gaaaacaagt gttttactta atctatacat tgatttttgt
     6661 ttctattctt tttagtttgt ttttaccttc aactaaggca tattatcata cgatagctaa
     6721 actttttcca tttttattag gagtttgggg gtattttaat cgaataacta ttggccgttt
     6781 ctttgaacaa aacactttta gtaaaatctg gcttttatta ctagctagtc tttgtctgat
     6841 tctgttcccg atttttcctt atacactaaa tgaactactg atcagtattt gttttgctct
     6901 attgttagcc agtgtcgatg atatgaacat tgcaaaaaag accaaaatcg ctagtacaaa
     6961 actttctttt gtaggaaaaa tcagttatga actttatttg gtgcatttcc cagtattgat
     7021 tgctctgaca aatctattca aacatcacca ttggaaatat ttggatatcg caggtctatt
     7081 cactgtgttt ttatgtacat tactcatgat tacttgcttt agaaaaggag tgcaaaagat
     7141 gagatcaccc aaaatttgga cagctatggc actaatgata atcggagttt ctgtactggt
     7201 attacgtgct cctgccagtc gactgaccgc tgaccaaaat cagttgcaaa gtttattaaa
     7261 agaaaactct agtaaagcaa atgcaggaga aaatctgttg tcacaagaga agaaaatatt
     7321 atttatcggt gattctgtta tgctgggggc gtatcaggaa ctaaaggaga catttaaaaa
     7381 agaagcattg gtagatgcaa aagaatcaag acaaatcact gcgttaccag aaatcttaaa
     7441 ggagcataaa gatttagttt cttaccaaca aatagtgatc gggttaggaa ccaatggtgt
     7501 gataacagat gaagctatag aacaaacaat gtctttgctg aaagataaaa aagtatactg
     7561 ggtaaatata aaagcaccaa ctggctggca agatacgata aatgccacat tagctacatt
     7621 gccaaatcag tattctaata taacgatgat cgattggtat agtgagagca aaaatcatcc
     7681 tgaatttttt tatgatgatg aaacccattt gaatgaaagt ggacgtacag cttatgcaaa
     7741 gtatattgct tcagttattc aataaggtag tgattacgtg tgttaacctt actttttcct
     7801 aataggtgaa tcaatttaaa taaaaaatac ttcatatgat aaacggtgtt taagaggagg
     7861 agctttttaa tattcgtggt aaaaaagaga tgggcgaggt cgttaactga gagcttcttg
     7921 gaaagaaatc gctttggatt tatcctcttt gttatacaga acatgagcga gacgacaata
     7981 ctaggatgta tgttctgatc gaagttttat ggttacaagc agcaatcagc ttagataaaa
     8041 ttaaggatcg taggaaccaa gtaaagaagg ctgttagtaa agaaacaaac gatcaagtat
     8101 gttttactct taagaagatg cagtgaataa ataatagtaa tataatgcta gaagggactg
     8161 tgacaaaaga cgtgtcacag ccttttttgt tattttaatt atcgtccagt ctttttttat
     8221 aaatgattgc ttaagcatta aatattaaaa aatatagagt tcaaaattac tgcacaatta
     8281 gatgaaaaat ttgaacaaca aaaagtgtat ttttatacta taatagcttt tgaatttagt
     8341 tactctattc aaaagttatg ctatggacaa gaaaaagaat aaatagaggt gagaaaatga
     8401 atagtttcaa gaaaaaattt gtgagtagat ttttaaatat gaaaaatttt tttgaaaaaa
     8461 agggagatta tgatgcatat aattatgtat taaatctaga aaagcaaaca tattatacac
     8521 tacgtaaaaa cgatgataat gacttttgga ctatgttacc taaaatgctt tcgattgact
     8581 ctaagctttc ccttttagag gaatttatta atttagacat tgaagaattt tgcgggggaa
     8641 aagagttaat taatatggta gagagtgatt atcataccta ttataaagaa agttgtggat
     8701 acaaagtgga tgagcaatgt ccacaatcaa ttatttttta cattgagtaa tatgaactat
     8761 taagcaggaa agcaactaga agctactgat ttagtattta caaatataaa ctatattgct
     8821 aacaattatt ttctagaagg aataaaagaa ctaagaatca agacgttatc ttgattctta
     8881 gttcttttaa catggaaaag atggataatg cactgttaat tcatgatgca tcatcagaaa
     8941 agttggaagg agaagataga aatcattaaa tgatttctac agatggtaaa tgaaaaaaca
     9001 aaatattagc aaaaatttgc ctaaatagat agtaacactt tctaaattaa atacaagcgc
     9061 attcataaaa tgtttataca aatatctatg tgataaaaat tggatatgtt cacaataaat
     9121 aggaattaaa ataacgaatt tacgtttgtt tttatttacc aatcaaatta tcttaatgca
     9181 ggaataaaaa attaaattac atattgttaa t
//
LOCUS       DANJMA010000078         9166 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000078 DANJMA010000000
VERSION     DANJMA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..246
                     /locus_tag="PWU31_002448"
     CDS             <1..246
                     /locus_tag="PWU31_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084926.1"
                     /translation="EKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDNCCSKYKVNQVRE
                     ALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            349..663
                     /locus_tag="PWU31_002449"
     CDS             349..663
                     /locus_tag="PWU31_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL1084927.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            641..2335
                     /locus_tag="PWU31_002450"
     CDS             641..2335
                     /locus_tag="PWU31_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1084928.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2355..3533
                     /locus_tag="PWU31_002451"
     CDS             2355..3533
                     /locus_tag="PWU31_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1084929.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3496..4182
                     /locus_tag="PWU31_002452"
     CDS             3496..4182
                     /locus_tag="PWU31_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL1084930.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            4182..5342
                     /locus_tag="PWU31_002453"
     CDS             4182..5342
                     /locus_tag="PWU31_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1084931.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5352..6227
                     /locus_tag="PWU31_002454"
     CDS             5352..6227
                     /locus_tag="PWU31_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084932.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6224..6535
                     /locus_tag="PWU31_002455"
     CDS             6224..6535
                     /locus_tag="PWU31_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084933.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6525..6878
                     /locus_tag="PWU31_002456"
     CDS             6525..6878
                     /locus_tag="PWU31_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1084934.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            6868..7269
                     /locus_tag="PWU31_002457"
     CDS             6868..7269
                     /locus_tag="PWU31_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084935.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7262..7666
                     /locus_tag="PWU31_002458"
     CDS             7262..7666
                     /locus_tag="PWU31_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084936.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            7678..8286
                     /locus_tag="PWU31_002459"
     CDS             7678..8286
                     /locus_tag="PWU31_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1084937.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGAAEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     SEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            8306..8668
                     /locus_tag="PWU31_002460"
     CDS             8306..8668
                     /locus_tag="PWU31_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084938.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            8671..8853
                     /locus_tag="PWU31_002461"
     CDS             8671..8853
                     /locus_tag="PWU31_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084939.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            8871..>9166
                     /locus_tag="PWU31_002462"
     CDS             8871..>9166
                     /locus_tag="PWU31_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084940.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLER"
BASE COUNT         3335 a         1324 c         1885 g         2622 t
ORIGIN      
        1 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
       61 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
      121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
      181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
      241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
      301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
      361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
      421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
      481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
      541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
      601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
      661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
      721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
      781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
      841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
      901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
      961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     1021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     1081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     1141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     1201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     1261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     1321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     1381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     1441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     1501 aactattaat tacaatgcag atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     1561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     1621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     1681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     1741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     1801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     1861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     1921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     1981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     2041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     2101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     2161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     2221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     2281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     2341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     2401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     2461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     2521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     2581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     2641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     2701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     2761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     2821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     2881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     2941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     3001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     3061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     3121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     3181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     3241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     3301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     3361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     3421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     3481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     3541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     3601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     3661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     3721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     3781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     3841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     3901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     3961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
     4021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
     4081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
     4141 gcagcattaa ataaaacatt tggacaagga gatgcaaaat aatgacaatt aaaaatttaa
     4201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
     4261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
     4321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
     4381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
     4441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
     4501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
     4561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
     4621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
     4681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
     4741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
     4801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
     4861 ctattacagc tttagatgct gtaactatgg gcggtttgat ggcgcgacta tcaaaattca
     4921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
     4981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
     5041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
     5101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
     5161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
     5221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
     5281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
     5341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
     5401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
     5461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
     5521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
     5581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
     5641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
     5701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
     5761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
     5821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
     5881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
     5941 tacaaatctg gacagccgag atgtgggcac agctttggaa catgatgtat ttcaatattg
     6001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
     6061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
     6121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
     6181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
     6241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
     6301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
     6361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
     6421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
     6481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
     6541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
     6601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
     6661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
     6721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
     6781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
     6841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
     6901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
     6961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
     7021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
     7081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
     7141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
     7201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
     7261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
     7321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
     7381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
     7441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
     7501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
     7561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
     7621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
     7681 gctaaaacaa aaactgtagt aacaacgttc gataacataa gtatcaaacg aattgctttt
     7741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta acggacaatt agatggcgaa
     7801 acagaaatgc aaacggtggt taaaaaatgt ggagcggcag aagtaaaatc aaaatctaaa
     7861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
     7921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
     7981 gtaagcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
     8041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
     8101 gcggatgaag tagctgcgtt agaactagaa gcaaaagtta tgcaagatga atttggtaaa
     8161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
     8221 aatctatctg ctgatgtgat taaaaaaact tcagttgtgg caactacggc cactcaatca
     8281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
     8341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
     8401 agattttaac acagacgagt ttgcaaaaat gtcgatggga aaaggctcta tggatattac
     8461 agttattcaa ggtgcaaaag ccgtatatgt agcttatcgt caagcgaaca tggtcgatta
     8521 catttcattc gatgaattta tagataaatg ggattttgat atggaggttg cagtagctgt
     8581 atacagtact atgatgttca aacaagcacg tgatgcctat caaaaagaat tcgaaaaagc
     8641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
     8701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
     8761 atctgtttag atgacatttt gcaaatggct tacaacaaga gcgcttatga aggatggaag
     8821 aactatgcaa taaatcaatc ccagaaaaac taaaggaaag gaggtaaaaa atggctaaaa
     8881 agagaacgga agcagaagta actttcatag ctaacgatga cggattgaaa tctacgttaa
     8941 aagaaatcag tgctgaatta actaaaaata gagcagaatt aaaactagaa caagctcaat
     9001 tacaacagac tggttctgaa tcagacaagt taggaagtaa attatcttct ttagaaaagc
     9061 agtatgaatt acaaagtcaa aaagttgaag taactagtca acgtttagcc aatgccaaaa
     9121 aatattatgg agaaaattcc accgaagttc agaaacttga aagaga
//
LOCUS       DANJMA010000079         8947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000079 DANJMA010000000
VERSION     DANJMA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(15..1154)
                     /locus_tag="PWU31_002463"
     CDS             complement(15..1154)
                     /locus_tag="PWU31_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL1084941.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="PWU31_002464"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="PWU31_002464"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL1084942.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /locus_tag="PWU31_002465"
     CDS             complement(2967..3722)
                     /locus_tag="PWU31_002465"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL1084943.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /locus_tag="PWU31_002466"
     CDS             complement(3826..5016)
                     /locus_tag="PWU31_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL1084944.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="PWU31_002467"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="PWU31_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1084945.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="PWU31_002468"
     CDS             complement(6168..7259)
                     /locus_tag="PWU31_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL1084946.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="PWU31_002469"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="PWU31_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL1084947.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="PWU31_002470"
     tRNA            8802..8873
                     /locus_tag="PWU31_002470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2321 a         1897 c         1648 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagaaa
//
LOCUS       DANJMA010000080         8905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000080 DANJMA010000000
VERSION     DANJMA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(520..744)
                     /locus_tag="PWU31_002471"
     CDS             complement(520..744)
                     /locus_tag="PWU31_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084948.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            935..3712
                     /locus_tag="PWU31_002472"
     CDS             935..3712
                     /locus_tag="PWU31_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1084949.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3793..4212
                     /locus_tag="PWU31_002473"
     CDS             3793..4212
                     /locus_tag="PWU31_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL1084950.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4224..4694
                     /locus_tag="PWU31_002474"
     CDS             4224..4694
                     /locus_tag="PWU31_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1084951.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4711..5490
                     /locus_tag="PWU31_002475"
     CDS             4711..5490
                     /locus_tag="PWU31_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1084952.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5477..6298
                     /locus_tag="PWU31_002476"
     CDS             5477..6298
                     /locus_tag="PWU31_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1084953.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6315..7364
                     /locus_tag="PWU31_002477"
     CDS             6315..7364
                     /locus_tag="PWU31_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1084954.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7377..8381
                     /locus_tag="PWU31_002478"
     CDS             7377..8381
                     /locus_tag="PWU31_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1084955.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2969 a         1472 c         1838 g         2626 t
ORIGIN      
        1 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
       61 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
      121 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
      181 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
      241 tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa aagaaaacaa
      301 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
      361 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
      421 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
      481 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
      541 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
      601 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
      661 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
      721 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
      781 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
      841 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
      901 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
      961 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
     1021 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
     1081 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
     1141 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
     1201 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
     1261 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
     1321 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
     1381 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
     1441 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
     1501 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
     1561 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
     1621 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
     1681 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
     1741 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
     1801 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
     1861 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
     1921 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
     1981 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
     2041 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
     2101 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
     2161 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
     2221 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
     2281 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
     2341 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
     2401 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
     2461 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
     2521 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
     2581 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
     2641 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
     2701 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
     2761 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
     2821 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
     2881 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
     2941 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
     3001 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
     3061 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
     3121 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
     3181 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
     3241 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
     3301 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
     3361 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
     3421 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
     3481 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
     3541 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
     3601 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
     3661 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
     3721 aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga
     3781 ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg
     3841 atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc
     3901 tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa
     3961 gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta
     4021 caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt
     4081 gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta
     4141 actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa
     4201 gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac
     4261 acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta
     4321 atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag
     4381 gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga
     4441 cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag
     4501 aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc
     4561 gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa
     4621 ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa
     4681 gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt
     4741 gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt
     4801 gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc
     4861 gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat
     4921 gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa
     4981 accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac
     5041 ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat
     5101 tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt
     5161 gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg
     5221 attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt
     5281 tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt
     5341 tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt
     5401 ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca
     5461 ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga
     5521 gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca
     5581 gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa
     5641 agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat
     5701 cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga
     5761 atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt
     5821 aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt
     5881 agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca
     5941 cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa
     6001 aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact
     6061 gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg
     6121 ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt
     6181 atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac
     6241 gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa
     6301 aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa
     6361 gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt
     6421 tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg
     6481 aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa
     6541 aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta
     6601 ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg
     6661 acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag
     6721 aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag
     6781 ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc
     6841 aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt
     6901 ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc
     6961 caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt
     7021 cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc
     7081 atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa
     7141 gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg
     7201 agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg
     7261 ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa
     7321 agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc
     7381 tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc
     7441 aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag
     7501 gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca
     7561 acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa
     7621 caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag
     7681 cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca
     7741 gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa
     7801 tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt
     7861 cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa
     7921 aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt
     7981 ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag
     8041 aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag
     8101 agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt
     8161 tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca
     8221 caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct
     8281 tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc
     8341 cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga
     8401 atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa atattggcat
     8461 caattacgtt ataaaacaca ttttttgcat tatctacagt tcaacttata atctgtttaa
     8521 tgggacatgt gggtgccaac cattttttgt tatgcaatag tactatttag ttgttgcatg
     8581 gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg ttttgtatcc
     8641 agaatgttga ctaaaacaaa agttcctatg tcaaccgtca acagagacaa tctgtccgtt
     8701 gcaggacctg acagatttag aaatgttcaa tctgtctctt atgtatttta aatcaatact
     8761 cactaggaga agtttcctaa cctcctaata gtaatgctgg tagtaacatt gtcaaaactg
     8821 cttcctctta ggaacaagca aagcataacg agtgtaaaaa taaaatgtag actattgtaa
     8881 catatcattg attatttcaa gtaaa
//
LOCUS       DANJMA010000081         8229 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000081 DANJMA010000000
VERSION     DANJMA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="PWU31_002479"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="PWU31_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL1084956.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="PWU31_002480"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="PWU31_002480"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL1084957.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="PWU31_002481"
     CDS             2591..3376
                     /locus_tag="PWU31_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1084958.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="PWU31_002482"
     CDS             3389..4240
                     /locus_tag="PWU31_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL1084959.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="PWU31_002483"
     CDS             complement(4364..4816)
                     /locus_tag="PWU31_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1084960.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="PWU31_002484"
     CDS             complement(5281..5649)
                     /locus_tag="PWU31_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL1084961.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="PWU31_002485"
     CDS             5799..6281
                     /locus_tag="PWU31_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL1084962.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="PWU31_002486"
     CDS             6318..7328
                     /locus_tag="PWU31_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL1084963.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="PWU31_002487"
     CDS             complement(7443..8156)
                     /locus_tag="PWU31_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084964.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DANJMA010000082         7597 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000082 DANJMA010000000
VERSION     DANJMA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(939..1352)
                     /locus_tag="PWU31_002488"
     CDS             complement(939..1352)
                     /locus_tag="PWU31_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL1084965.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="PWU31_002489"
     CDS             complement(1808..2293)
                     /locus_tag="PWU31_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084966.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2647)
                     /locus_tag="PWU31_002490"
     CDS             complement(2432..2647)
                     /locus_tag="PWU31_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL1084967.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="PWU31_002491"
     CDS             2871..3671
                     /locus_tag="PWU31_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1084968.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..4820
                     /locus_tag="PWU31_002492"
                     /pseudo
     CDS             3690..4820
                     /locus_tag="PWU31_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            5137..5559
                     /locus_tag="PWU31_002493"
     CDS             5137..5559
                     /locus_tag="PWU31_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1084969.1"
                     /translation="MWKEKKTYQEYLNKLVAPLIRGYYVDEEFEKRILKREYQQSTFV
                     NKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFLFAIPEEMTAEVENELFEL
                     YDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="PWU31_002494"
     CDS             5552..6043
                     /locus_tag="PWU31_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL1084970.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="PWU31_002495"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="PWU31_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL1084971.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="PWU31_002496"
     CDS             6603..>7597
                     /locus_tag="PWU31_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL1084972.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2234 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DANJMA010000083         7283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000083 DANJMA010000000
VERSION     DANJMA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(170..3319)
                     /locus_tag="PWU31_002497"
     CDS             complement(170..3319)
                     /locus_tag="PWU31_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1084973.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="PWU31_002498"
     CDS             complement(3333..4550)
                     /locus_tag="PWU31_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1084974.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="PWU31_002499"
     CDS             complement(4540..6135)
                     /locus_tag="PWU31_002499"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL1084975.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="PWU31_002500"
     CDS             6260..7045
                     /locus_tag="PWU31_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HDL1084976.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DANJMA010000084         7144 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000084 DANJMA010000000
VERSION     DANJMA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..1294)
                     /locus_tag="PWU31_002501"
     CDS             complement(<1..1294)
                     /locus_tag="PWU31_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1084977.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            1613..2338
                     /locus_tag="PWU31_002502"
     CDS             1613..2338
                     /locus_tag="PWU31_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084978.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            2328..2837
                     /locus_tag="PWU31_002503"
     CDS             2328..2837
                     /locus_tag="PWU31_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084979.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2907..4355)
                     /locus_tag="PWU31_002504"
     CDS             complement(2907..4355)
                     /locus_tag="PWU31_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1084980.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4355..5071)
                     /locus_tag="PWU31_002505"
     CDS             complement(4355..5071)
                     /locus_tag="PWU31_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084981.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(5052..5402)
                     /locus_tag="PWU31_002506"
     CDS             complement(5052..5402)
                     /locus_tag="PWU31_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL1084982.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            5546..6319
                     /locus_tag="PWU31_002507"
     CDS             5546..6319
                     /locus_tag="PWU31_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1084983.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            6490..6856
                     /gene="ssrA"
                     /locus_tag="PWU31_002508"
     tmRNA           6490..6856
                     /gene="ssrA"
                     /locus_tag="PWU31_002508"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2088 a         1392 c         1273 g         2391 t
ORIGIN      
        1 atttggttaa aaatgttttg ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt
       61 tgcgaaagcc gtacgcattg cgttttaata ctttgatgtg gttgtttaag cattccaatg
      121 gtccgtttga atagctgtag tgaagcgcat tttggatctg tggcagaaac tttttaaacg
      181 ttcgtaaagt ggttgtatag gtttctggca gaccgctaat gtctctagtt aaacaatcct
      241 caaatagtga ctgatctctt ttctttaccg cataaagaat gtcttggtat aactgatagc
      301 cttggcgtaa gttgggacaa gcagctaaca gtcgatcggc aatctcactt tcagtcaaat
      361 aatctttaaa actggcacgc caaacccgtt tttcatagtt taactttgct tcttcttttt
      421 gtaggagttt ccaatagcgc ttcaattttt tccctaaccc tcgttgagag tgggaatttc
      481 ctttaagtcg taccgtggtt tctttcactc gatgattcaa gaaagttctg ccaatatgtt
      541 ggacgatgtg gaaacgatcg atgatcaact gagcattcgg gaaaagcttt ttcaccaacg
      601 aaacataagg cgcatacata tcaataacaa tcaattgaac ggcttcacga gtcgctaatg
      661 aaaaccgaga aaaatagcgt tctaaataag gcaagcgtcg attttcgaca atatccaata
      721 atgcatggct ttgtccgtcc atcatgataa aactcattgc actggcaaca cttcgaaccg
      781 atttgaattc atcaaagcat aagacctttg gcaaagtctg tttgaaagga ttcaatgggc
      841 ggtagaatcg cttcatgaca cgatagaccg aagaagtgga aatatttttc tgacgagcga
      901 tttccgtcat ggaagtattc tcttttaagc gagcaatgac agaccaacga actttttgac
      961 taatgaaaca gtgtttttca gcaacagagg tatccgctac gaatgttctt tggcaagact
     1021 tacatttaaa tcgttgtttc ttcagctcca gataagtgcg atactcagac acatcattca
     1081 gtaacaaccg aacggttgtc gtcccccaac gaatgatcct agaggggacg acacaaagac
     1141 aatgggaaca ctcttctggc ttgtacagaa gagttccttt gtaaatcaag caaatttgcc
     1201 ctttgtattt ttctttagtt aaacaatttt cctcgaaata gatatttagg tctagaatat
     1261 ttagggtatc tttgataagc tgagtatgag acatcttgaa tcctcctatg aattggtagc
     1321 caaattaatt ctaaaggatt tcagggtgtc ttttctatat ttttttatac aaaaaagatg
     1381 ttggtgaagt tcaccaacat caaaaagtat agagcccaaa attattgtcg ctgctcctct
     1441 tttcgaataa acagtgttca aaagcttgca tctacggtaa tagaataggg cagcttcttc
     1501 acaacctctt gctttttaca aaatattttc tggttttaat tcgtgatttc actaaaggaa
     1561 tttcatactt tattcatatt cattttctat actttttata gaggtgaaaa gtatgaaaat
     1621 aatccgctat gctactgctc gattaattta ttacaaaaag cagacaatac tctattgttt
     1681 attttcaagc ttttctgctt ttttattgat agcatgcctg actctttacc attcgcaatc
     1741 ggctttagcc aatcaagtcc aagatcggct ttcatttatt ggcgcttctg catctacaga
     1801 gcttcttccg gctttagaat tagctagccg attttatata aaaggcatat tcagtttatt
     1861 ttttctattt gccttccttt ttatcttttt cttttactat tcagtcaggc aaaacaagca
     1921 ggaattgatc aattggcgcc tgactggtct ttctaaaacc aaattattcc tttttatttt
     1981 ctggcagctg ttctttcctc tctttctttg ttgtatcttg gttttattgt ggatcgtcag
     2041 cttccagtca gtttatgaag cgcttcttca aaggatcaat tttcttgtct taaagactac
     2101 tgaactgcca gatatccgtg atatcttgac ttcggtaagc ggacttacaa ttccgatgga
     2161 tcaacagact ttttttacca tcgatttcca aaatgatctt tttttcacag atatacttaa
     2221 gggatttgcc caaactctta ttgggctcag tggatgctgt ctcattctta gtctattccc
     2281 tttcactttt tttatccatc atttgcaaag taggaggcct ggacgtaatg aaacctgaat
     2341 atttgattca tgctgaacac ttgaccgtta ttttgtgtcc tctcgaaaaa cagcataaaa
     2401 tattggagca cttgaaaaaa gaggttcttg ttgcaaaact ggataatttt gcgatcatcc
     2461 aaaaaaattg gccgatgttt ccttatctga atcttaaaga ccatgtcctg ttggatgtac
     2521 cggaaaaaga aatcaaacaa gatcgtttgg cctatcaaga aaaacttgat atctctcctt
     2581 ccttgcttaa ttgtgcggca gctgagttga cgtcttttga aaaagtaaag ctgcaattac
     2641 ttcatgcctt gctttccaag cgaaacaata ttgttattga agatacattt gatgaactta
     2701 gtgtattgga gatccaagag ctactccact tgctttctta tttagctcat gaagaaaatc
     2761 aaggtatctt actctttact catgatgcta ctattgctca aagtccttat attgatcgtt
     2821 tagagcccgc gggctaaatc aatcatttta tagtaaaaac aagcggtata gaagatcgat
     2881 tcatgatctt ttataccgct tgttttttac tcttttttga tttctacgtc aggcaaacga
     2941 acagcgaatt ttgttccttt tcctagttcg ctttctactt ctaattttcc tttatgcaag
     3001 cgtacaagtt gctgcacgat tggtaaacct aatcctgatt caccatattt tgtatttttt
     3061 cttgaaggat ctactttata ataacgatcc cagatacttt tcatttgttg ttcagacatt
     3121 ccaattcccg tatcttcaat cgtgatgatc gtttcaagat agcctttttc taaagtaatc
     3181 gtgatttggc cgttttcagt aaactgtatc gcgttttgaa taatattgac catgatttgg
     3241 acaaatcggt cataatcagc gtaaacatca atatcatcaa cggctttcaa tgtcaatgta
     3301 tctcctgctg cttctgcttt agcttctaac tgtgcaacaa tatttttcaa tgtttctgtt
     3361 ccattgaatt ttttaatgac catactgatt tgattcgtac ggattttttc ataatccaag
     3421 ttttcattca ccaaacggat cagacgctct gtttcatttt tcatcaattt aatcgcgttt
     3481 tctcgctgat tttcagggat tgcattgtat tctaaccctt ccagaagtcc gttgattgtc
     3541 gtaagaggtg ttctcatctc gtgagaagca tctgccataa attgtcttcg acgattttct
     3601 tgttcttcaa tttctgcttg agattctttc aatgaattcg tcattttatt gaaatcatct
     3661 gctaactcat caaattcatc tttgtcatgt acggggagct gtacatcaaa attaccattc
     3721 gtcacttctt tcgtcgcatt ccttaaccga ttgatacgct taacttggaa tgctgcaaaa
     3781 gcatagctgg caataactgc aacgatactg gaaaaaataa atcctttgaa caggttcagt
     3841 gtaattggac gaacgctgtt atcgatattt cgcgcaggtt gactaactac aagtgctcca
     3901 tagaattcat gattcaaatt aaatggcaca agtgcgtaag aggtcgcctg tgcttgtcct
     3961 aaaatatttt tattcgatgt gaatttttct tgacgtccat tcctcagatt tttccattga
     4021 tcagctgtta aggtaaaatt cagtaaagct gtattagtcg gataaatgac acgttcgttt
     4081 ttatcgataa atataaagtt cacattttgt tctgtcagta cttgctctgt cagcagcaat
     4141 gcattttgaa acgcctgatc ttgactaaac tgcgggagcg tatcggcata ggtttgcgtt
     4201 gtcttttcaa ctgattcagc atatccaaac agttgctggt aattgttttc ttcaatcgtt
     4261 tgttttgtca gttgtgtaaa agaaactcca acgatcaata agatcaacgc gaccattccg
     4321 caaaaagcta agagttgctg atacaaataa cgcattaggc aacaccagaa tcatcgaatt
     4381 tgtagccgac tccccaaaca gtttggatga cttgtggtcc gaccttttcg attttttgtc
     4441 gtaatttctt gatatgggcg tccactgtcc gttcatcacc aaagtactga taatcccaga
     4501 ctaattcaag caactgctcc cgagagaata cttgacgtgg tttttttgct aaagtaaaca
     4561 atagatcgaa ctctttaggc gtcagtcctt caattggccg gttgtccaga tacgcttctc
     4621 gtgttttcgt attcatcttg aaatgactag tctcgatatc aaaatgatca tctgaaactg
     4681 ctgcttcttc tcttgcttct gccaactcac tacggcgatg caaagctttc attcgagcaa
     4741 taagcgtcag aggactgaaa ggttttgtga cataatcatc tgcccccatc tctaagccga
     4801 tcacctgatc gctttcagaa tctctagctg tcagcatgat aatcggcaca gtacttgaaa
     4861 ctttgcggat ctctcttcct actagcatac cgtccagtgt gggcaaattc aggtctagtg
     4921 taatcatatc ccaatgttgt gggttttcta aaaaggcatc gagcccttct tttccatcat
     4981 acttgaaagt tgcttcccaa ccttcattta agaaaaacat ctgcatcatt tcagaaacag
     5041 attgattatc ttcaatcatt aaaatattca ttatcgttcc ctccagtttc caggtcatat
     5101 ttctgtaatt tcttttctgt cataataaaa ataggcataa ttggaaatac tagcagaagc
     5161 atttcaatca gaaaataatt cgtatgccca gatgtcttta aataatagcc gatcaatgcc
     5221 atcagtaagc ccataagaaa aaaccagttt ccgcttgtct tttgagccag ccgccaatga
     5281 gcgtctgttt ttgcggcaag aggcgaatga tacccataaa aaggcagatt tccttttgag
     5341 ggcagaatcc gaaaaagact gccaattatt aagaataaga taccaatact gaaaaaaatc
     5401 attctttccc tcctaaattc tcttcttata gtatactcta aattgttgac tgaaccaata
     5461 tgttcaggaa ttcttcacga aaggtttgtc ttttgttcat tctttgttaa gataaactta
     5521 ttccaataaa aaaggagttt tatttgtgtc aaattatcgt gcaatcactt tctttgatct
     5581 tgatggaaca ttactagatg cgaaatcaaa aatcacaccc gaagtggcaa aagctatgaa
     5641 cgcattaaaa gaaaacaatg ttctccccgt catcgctaca ggacgtacag aagcagaaat
     5701 ccactctatc atggaagatg cgggcattac ttctgcggtc accatgaatg gttctttcat
     5761 tcgagtagac ggcgaagaaa tctattctca aacattttct tcagaagaat gtcaaaaaat
     5821 gtatgatcat gtacagttga acggtcatga actttcttac tataacgatc agaaaatctg
     5881 gtgtaccggt catagccaaa caatgatcaa tgcttacgac ttcattcatt cagatgttcc
     5941 ggaaattgat cctttaggct ttaaagaaaa cacagtaaat atgttgctga tcttatctca
     6001 atcaggtgac gaatactatc acgaacattt tcctgagcta actttttatc ggaatggtcc
     6061 ttactcgatc gatacagtga agaaaggaat ctcaaaaggg gcaggtgtca aacgtctgca
     6121 aaaagaactt cagctggaaa aagttcctac ttttggattt ggcgatggtc cgaatgattt
     6181 tgccttgttg gaagcttgcg acaacaaaat cgctatggac aacgcctatg acggattaaa
     6241 agaactttct acctttatca caaagaaaaa tacagaaggc ggaattgtcc acgcattgaa
     6301 gtattttgat ctgatttaag atcttttttt gaagctacct cttttcttaa gaaaatcgct
     6361 tattttttct taagaaaggg gcagttatta tataaataat gggatatgat tcgctttttt
     6421 attgaattta tcccaatata tgctatacta attttgtatg aaaaatacac gtaacccttg
     6481 ttagctaacg gggacgttac ggattcgaca ggcacagtcg agcttgaatt gcgtttcgta
     6541 ggttacgtct acgtaaaaac gttacagtta aatataactg ctaaaaacga aaacaactct
     6601 tacgctttag ctgcctaaaa acagttagcg tagatcctct cggcatcgcc catgtgctcg
     6661 agtaagggtc ctaactttag tgggatacgt ttcaactttc cgtctgtaag ttgaaaaaga
     6721 gaacatcaga ctagcgatac agaatgcctg tcactcggca agctgtaaag tgaatcctta
     6781 aatgagttga ctatgaacgt agatttttaa gtggcgatgt gtttggacgc gggttcgact
     6841 cccgccgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa acgttgttaa
     6901 atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa attttttgca
     6961 ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg tattcaggaa
     7021 aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg aggtgaactt
     7081 ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca aataagtttt
     7141 ttta
//
LOCUS       DANJMA010000085         7026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000085 DANJMA010000000
VERSION     DANJMA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(57..350)
                     /locus_tag="PWU31_002509"
     CDS             complement(57..350)
                     /locus_tag="PWU31_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL1084984.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="PWU31_002510"
     CDS             complement(378..1043)
                     /locus_tag="PWU31_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL1084985.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="PWU31_002511"
     CDS             complement(1183..1815)
                     /locus_tag="PWU31_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1084986.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="PWU31_002512"
     CDS             complement(1822..2889)
                     /locus_tag="PWU31_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1084987.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="PWU31_002513"
     CDS             complement(2886..3617)
                     /locus_tag="PWU31_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL1084988.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="PWU31_002514"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="PWU31_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL1084989.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="PWU31_002515"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="PWU31_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL1084990.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5740..6900
                     /locus_tag="PWU31_002516"
     CDS             5740..6900
                     /locus_tag="PWU31_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL1084991.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         2076 a         1472 c         1086 g         2392 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atccatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacagctc taagtagtct agaatattct ttgatactaa gttatgtgca
      661 atccgtatcc ccatgtctcg ctcagggtgt acgacacgat ctgctccgat cttctctagt
      721 acacgggcat ggtactcatt ttgggctttt gctaaaatat tcggtacgcc catttccttt
      781 accatcagag taactaagat gctggcttgg atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatacctaa tgaacgaagt gtcatctcat cctgtgcatt tgccacaaca
      901 gcatgggtgg ctatattcat atattcattg actcgatctt cattactgtc aatagccagt
      961 acttcttgtc cagcttcgat cagtgtacgg cagatgcttc cgccaaatcg tccaaggcct
     1021 acaattgcaa aattttgttt caccattttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt ccaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttaaacgca tagatagctg cttgagttcg gtcttctacc tctaacttag ctaatatatt
     1261 cgacacatgc gttttgactg ttttcagtgt gataaacaat tcatccgcga tttcctgatt
     1321 acttttccct tcagaaatca gcatcaaaat ttcattttct cgattcgtta attcttcatg
     1381 caatactgga tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttcaggttc
     1441 tagtactcgc tcgccgcgtt gcgtagccct tatagcatca gctatttcat gtgctgtaga
     1501 cgtcttcaac aaatatcctg ctgctcctgc ttcaattgca ggataaactt tttcatcatc
     1561 aataaaactt gtgacgataa tgattttggc ttttggccaa tcttttaaga ttgcttttgt
     1621 tgattcgatg ccatccattt cttccatgac tagatccatt aaaatgacat ccggtcgaag
     1681 tgccatcgcc ttttcatagc cttgacgtcc attttcagct tctccaatca cttcaatgtc
     1741 ttcttggata gataaataag aagagacgcc taagcgtacc atttcatggt catctactaa
     1801 taaaactttt atcatcttta tctatgcctc cttcatcaaa gggaccttgg tctccacgct
     1861 cgtaccttgc cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttggtttca ttcatattaa atcctgtccc
     1981 gtcgtcaatg atacgtaata aaagattgtt atctatttta tgcaaataga cttctaattc
     2041 attagcctta gcatgtctta atgtgtttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagtta attttacatc ttctacctcc cattttagcg aaatctggat
     2161 tttattctgc aattccttca ataactgctc aatgccttgt tttaaacttt tttcttctaa
     2221 attgactgga cgcagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgccacc attttcaact gcttacggaa catttctggt gtctcacttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcatcgc tgcaaataat tgctgtgaaa ctgaatcatg
     2401 aagctcacga gctaatcgat gcctttctaa ttttaaaatc tcttctttgg tttgtccatc
     2461 tacatattga ggacggctag tcataatctg taattcactg gatacttcga tcattttttc
     2521 ttttatttta gtaatttcca tatcaatatc tttgatgtac agatcatccg acgcgcttgg
     2581 gatcggctga tgcaaaagtt tggattcata attgccagaa gaaagtgcac gaagcttttc
     2641 ttcgattttc ccgtattttg ccttttgaat aagtgataac aatagaaata cgatcaatcc
     2701 tacacctaat gaaattgcca tcaaatggaa gataagcgga acgtataata aacgcgcacg
     2761 cagcaattct ccccaccaat gactttggtt actggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcattgctt tggatattct
     2881 tcccatcata ttcgtatcac ctcgacatct ccaaccaaag tattggtaat gatcttcaat
     2941 ctgcgaggat tttcatcata atcactgctg cagattttga tctgttcgtt ttttaaggaa
     3001 aactgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa acgaatagcc
     3061 actcccaggg gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagtgt attgcccaaa tcgatgatag tatcaccaga aatgatggca
     3181 atgttgatat catcccattc atagacgtcg tttccaatac gttggttacc aaataattgc
     3241 tgtcttttac gctcattatt atgtgttttt aattgctctg tttggaccat catgatctgt
     3301 ttttttcgcc aaaaagcatt ttttgtcaaa tctattcctg aaatctctac acctttcagc
     3361 ccgataaata aaacggccaa tacaaccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaagctccc taagatcaat tgaaaatttt gaaatttagt acgaggatat
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatta ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagcagtg cttcgactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tttttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttgac tgttggctat aggactttag atgtaagatc gttcagtgaa acctgcaata
     3721 ctcaaagaga aaagatctag agtgcgtcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggtgtagcaa
     3841 tggttacttc atcattttta cgtttgccaa tcaatgcttg cgcgatagga gaatcattgg
     3901 aaatctttcc agaaaacggg tctgcttcag cacttccgac aatcgtgtac tcttcttcat
     3961 ctccatcagg caactcgatg aacgtaactg ttttaccgat cgatacctca tctgcatcta
     4021 cgccgtcatt atcgatgatt tgtgcaaaac ggatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttagctg attcgtattc ggagttttcg gaaagatctc
     4141 cgaaaccgcg agcgattttg attcgttcaa tgatttctcc gcgtttaact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt aaaatgtaaa tgtttttcta aaaatgatca
     4381 atgactagcc cctgtttttc taagaattat ttacatattt ttgcactagt tccatatgtt
     4441 cttcatacgt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gaccaggccc atagccggta ttggtataca aattgtatgg tgaatctaca gctgtatctt
     4621 catacgtcac caattcttta tgttctccta atgcatataa aacagagata tctgactgaa
     4681 gtggcatatt tgccttgatt cggttaaaga agacttgtgc aatattttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ccaaagttaa aacttcttgt acagtcaaat
     4801 ttttctgttt gatagctgaa taatagtttt gcatgaccgt atttgttttg ttcaccatct
     4861 caatcacaag atccttaagc gaagtactcg tgtagtaatc ataagtagct gggaataagt
     4921 agccttccaa acggtatttt gtgttctcag ctttagaggc actttccaac aattctggga
     4981 atttttgata caactcgtta aagaaagtct catcatttac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgct cagcaatctg atcgatgtcg tagccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtgccgcct tcttgcagct
     5161 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
     5221 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
     5281 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tccacttgga
     5341 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
     5401 taaatccaag aaatccgcca ataattaata atgtaagagc aatcactaaa atgatcttcc
     5461 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcatctt cgtaactctg tttattatac atgaaatttc tactgccgtg
     5641 aattatttta ccaaatatta tgtcaaaatt gtaagtggca aatactcttt ttgaggatta
     5701 caatgaaaaa tagttaaaaa tggatagaaa ggaattaaca tgtcaaaagg aaaacatcga
     5761 aaaaaacaaa aaatttctat ttcttttctt gtaattttat tattgtctgt cagcttcttt
     5821 tcaacaggca tcattatagg aggtcaatta acaaaaatac ctcattccca aacttataaa
     5881 gcggagccaa cacaacttac ttccaattta acgcccgatg agcagaggct tgctagcaaa
     5941 attgatcaat tactacaaaa atcagattat atcggttcga tttatgttcg caaaaataat
     6001 cgcgtactat tagaaaaagg atatggatat gcaaatctgc gcgtaaaaga atcgaattca
     6061 ccttctattt actatcaaat cggttcgatt caaaaagcaa tgactgcttt actgatacta
     6121 aaacaaatcc aagctggtaa gttaactttt gacacaaagt tagcagaatt ttatcctcag
     6181 attcctggaa gtcagcaaat cacaatcaaa aatatgcttt atatgcgctc tggtcttcat
     6241 cgaactgctt ctcccgacaa acctatgtcg gatagtgaaa tcattcagtt cgcccttcat
     6301 catttgaaac ttactgatta tgatacgtat cattatgaac cattgaatta tacattgctt
     6361 acagggatat tgatcaaact aacgaatcag ccttatgaaa ctcttctaaa aaaagaaatc
     6421 attcgtccac tccatctaga acatacagat ttttatgaca atgtaaaaaa ttcgcctcaa
     6481 catgctgtat cttaccagat gtctgcaact gacgattatt caaaagcatt gatcgagcct
     6541 gaaaccgaca tccgaaatga attaggtaca ggaaacataa gtatgtctgt ttatgacttg
     6601 aacaaattct ttaccagtgt tctttctggc ggtatcatac caaaagaact acttttctca
     6661 ttgtggcaaa ataatggtga taaacatcct tacagcggcg gtgtttacag tggaaacgat
     6721 tatatccttg ctcaggggaa tatcaatcga ttccatgcag tagtggcgat gaaaaaagac
     6781 actaaagatg caatcgtcat ggaaagtaat atccaatcgg ataagaacat caaattgccg
     6841 gccactgatt taagaaatca gatttatgaa ctgatggaag gtgttcactt atcaaattaa
     6901 attttgacat aatattagtt gtcatctctg tatagtcaat ttagttagta ctctatctga
     6961 aaagtagaaa taaattaaca aatattatct gtccttatta tttgttaatt tatttcttat
     7021 tttact
//
LOCUS       DANJMA010000086         6990 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000086 DANJMA010000000
VERSION     DANJMA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            302..898
                     /locus_tag="PWU31_002517"
     CDS             302..898
                     /locus_tag="PWU31_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1084992.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(956..1438)
                     /locus_tag="PWU31_002518"
     CDS             complement(956..1438)
                     /locus_tag="PWU31_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084993.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1544..1741)
                     /locus_tag="PWU31_002519"
     CDS             complement(1544..1741)
                     /locus_tag="PWU31_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084994.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2036..2794
                     /locus_tag="PWU31_002520"
     CDS             2036..2794
                     /locus_tag="PWU31_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL1084995.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2854..3282
                     /locus_tag="PWU31_002521"
     CDS             2854..3282
                     /locus_tag="PWU31_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084996.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3328..3400
                     /locus_tag="PWU31_002522"
     tRNA            3328..3400
                     /locus_tag="PWU31_002522"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3361..3363,aa:Gln,seq:ctg)
     gene            complement(3550..3921)
                     /locus_tag="PWU31_002523"
     CDS             complement(3550..3921)
                     /locus_tag="PWU31_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1084997.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4136..4321
                     /locus_tag="PWU31_002524"
     CDS             4136..4321
                     /locus_tag="PWU31_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084998.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4404..4592)
                     /locus_tag="PWU31_002525"
     CDS             complement(4404..4592)
                     /locus_tag="PWU31_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1084999.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4616..4879)
                     /locus_tag="PWU31_002526"
     CDS             complement(4616..4879)
                     /locus_tag="PWU31_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085000.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5037..5294
                     /locus_tag="PWU31_002527"
     CDS             5037..5294
                     /locus_tag="PWU31_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL1085001.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5365..5556)
                     /locus_tag="PWU31_002528"
     CDS             complement(5365..5556)
                     /locus_tag="PWU31_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085002.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5697..6017)
                     /locus_tag="PWU31_002529"
     CDS             complement(5697..6017)
                     /locus_tag="PWU31_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085003.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6040..6474)
                     /locus_tag="PWU31_002530"
     CDS             complement(6040..6474)
                     /locus_tag="PWU31_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1085004.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6809..>6990
                     /locus_tag="PWU31_002531"
     CDS             6809..>6990
                     /locus_tag="PWU31_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1085005.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2190 a         1362 c         1070 g         2368 t
ORIGIN      
        1 taaactaatt ttctataaaa gcaagtaatt tttaatggca atatcataaa aaaaagaaga
       61 tagagattta ataattatat atcagctgtt tttttcaaat aagcaaaaat tcctaaaaca
      121 caacaatgaa agcgataact aagaaaatcc ttacataacg atttttctta ttaaaattgt
      181 tttttgaact tcgcttattt tttgtgagca tatctgttta cttacttttt gtaaggtgct
      241 atacttcatt tcgtaatcaa ttaaaaaaat gcaaaacgca aatcaaagga gaaattttat
      301 tatgacaact tttactgaca ctttgaaaaa ccgccgttca atctatcatc taggacgtaa
      361 tgtttcactt tctaatgaag aattaacaac tttgatcaaa gaagcaatta aagaaagccc
      421 aactgctttc aatgctcaat caacacgcgc agttatcctt ttcggagatg cacatgaaaa
      481 actttgggaa atcactgaag aagcacttcg cccattgact ccagcagaag ctttcccaaa
      541 tacacaaaac aaattagctg gtttcaaaaa tggttatggt acagtattat tcttcaaaga
      601 tactgacgtg gtaaaaggtc tacaagaaca atttgaatta tatgcagaca acttcccaga
      661 ttggtcagaa caatcaaacg gaatcgcaac agctaatact tgggtagcat tagttgataa
      721 aggattgggc gcaaacttgc aacattacaa cccagttatt gacgaagcag ttgcaaaaga
      781 atggaatatc ccttcaaact ggaaactacg ttcgcaatta gtattcggtt caccagaaac
      841 accggctggg gaaaaagaat acatgaacga tgccgatcgc ttccgcgtat ttggataatt
      901 ttatttgaaa taaaaaacag aggttgtgac aaacatcttg tcgcaacctc tgtttttatt
      961 ctggatcaat cataatatca atctctgcct catcgtttaa gcgttgggac aaaaatggaa
     1021 ctaattcttc taatgatgca gaaaatttca gcatcggtcc tcgccatccg tttcggaaaa
     1081 aattcatttt tacgatttta gaaccttttg gctgatataa tacaatttta ttgatctcag
     1141 aaaaaggaac accttttttg tcaaagctat tcaaaacaag tccttcttct gtgattcctc
     1201 tactgtccaa caaaaaacta ataaaaatca ttgctgacaa gatccctttt gccaattcat
     1261 ctgctgtagg atgactgatg agtgtatacc aaatgactag tgacactaag aagggtatca
     1321 ccaagtagac ccatcgattt cgcacagtcc gataaataat ttcttttcga accagccaaa
     1381 gaaataaaaa catcacaaca acgaataaaa acaatagaac cgtaaagaat gttaccatga
     1441 caactcgcct cgctttttct tctccagaaa ataggaaaag aatactgatt taccttctca
     1501 aagaaattgg acttgcagca cgccgcacgt ccaattgttt tctttattca aatgtatcat
     1561 attttgcaac aaccatgctt gctttttcat ctaataattc ttggtcctct gcagcacatt
     1621 gggcaacttt gaccacggcg cagtttgtca tttttgatac aacttcacca atgaccgttt
     1681 ttgtaaaacc aattggtttg catgcatatt gttctcctaa attaatcatt tcttccgtca
     1741 tcaaaatcct cctaaactgc tggaactttt ctccacatta ccactgctat ttctttggag
     1801 atttaactca ttgattttta tttacagtta aaaatcaacc ttacaggcat tatagcgtac
     1861 tgttttcgtt ttcacaatta aaacagctgt tgtatttaga agaatttttt tcaaaaaatt
     1921 aaaacatatt ccttaaaaac taattatttc ctacatatta aaatataata cttccatttc
     1981 caataaaaat acatttataa taggaaagat aaacagctga aaagggagat ttcaaatgaa
     2041 gaaaaaaacg ttcaaaaaac tattgtttct agggggcaca gctgctcttt tattaagcgg
     2101 tacaagttat tacctacata tccattctcc agaacataca atgaacactg cacaaaaaag
     2161 ttcaacacaa aaaacgaaaa agacaaaaga gaaagcagaa gttttactag atgttccatt
     2221 agagagccag tttgattctc cttctttaga aaatggctgt gaagtcactt cgctttctat
     2281 gcttttatct ttttatggtt atgaaacaac taaaaaccag ttagctgagc aattggatta
     2341 cgtccctgtt ttcaatgcag atgggactca tggcgatcct aatgaaggat ttgttggtga
     2401 tatcagtggc ggagactggg ctatggtcgt ctatgttccc ccagttgcct cccttgctca
     2461 gcaaatcgtc caaacggatt atcacacttt tccaaaaacc gatgctcaac tcgatgatat
     2521 caaaaaagca ctatctaaag gaaatcctgt ctggacttct gtgactatca attttgaagt
     2581 cccagatgaa actgatttta tgacttggac cacaaataat ggagaagtaa gagtcactcc
     2641 tcttgttcat gcttgtgtag tcactggata tgacaaagaa aatatttatg tcaacgatcc
     2701 ctatggtgta aaaaatcgag cagtaccgat caacgatttt tcggcgattt tcactgcaat
     2761 gggtggacag atgctaactt tggagaaaag ctagtaattc aaacaaaaat cccttatact
     2821 caagatacca aacctttgaa gggagcgtag ataatggaaa aattcacaca taaaaaaatg
     2881 gatccaaatg aaattccgat tatttttgtc cgtgactgca agggaaatgt tcaaggtaaa
     2941 gtctctatta atgagtggaa cgagcgtcgc agacctgcta ccctcaatga actggaaatc
     3001 aaattgtatc gtcaatctct tgtttattac gcagatcaag agtacgagaa agccaccgat
     3061 ttattgaaat ttctaattgc tcgaacagag tatactcgct ttgaatatat tgaacgttta
     3121 gcaaacatct atcatattat gaatgaacct gtcaaagaat atcagttatt ggacaccgtc
     3181 ttgtcagtag cagaactgat tgcccttcct gctggattag aaaagaaact agtacgccgc
     3241 cttttacgag taaagcaaca actatccgat caagaaaaat aacttgcgtt tttttaggaa
     3301 agtgctatga ttattttgtt gaataaatgg gatgtagcca aactggtaag gcacttgact
     3361 ctgaatcaag caattgtagg ttcgaaccct gccatcccaa tttgatgtgc ttgctgaaaa
     3421 aatgaacccc ctgtaaaaga acaatctttt tgcaggggga ttattgtatt atttttacta
     3481 cttgtattta gtttacataa ttaaattatg aataataaaa tcgggcgtat tcttaataca
     3541 aaaactgatt tacccaataa ttgctaatgc tttctccgga actttttctc taggaacttc
     3601 ctcatacgtt tctatgtgtg ctcctttatt gctaattttc aaataatgat tttcttttaa
     3661 cacagaaaga ccattaaatt caaccgttcg ttctttccca ttggcatcag cagctttctg
     3721 cacatacctt gctgtaccat attcatttac ttcctctggt ttttgtgtct ttacatagac
     3781 ctctcctttt tcaactaatg gatttagctg atcaagtata ccagggattt ctccataact
     3841 tccttcagta taatatttca gtccaaatag caaaatacca ctaatcgccg ccagtccaat
     3901 caaatattta atcactttca tctttctacc tccttgttat atattcagta tatgggtcag
     3961 ttaaagaaat tccatccgtc ccaagttgga gatttttctc ctactttaga agcgagaatc
     4021 gagtgttttt ttagtaaaaa tgatcgttat ttgatattat tgacgtgtgg ataaatcagt
     4081 ttttctcgtt ttgtccatgt aaaaaatcat gttgatagca aaggatgtgt tcaaaatgaa
     4141 tcaagaagaa ttatttcaaa aagtcaagga aatgattaag aacggcaatt tcgatggcgc
     4201 aaaacgattt atcgaagaac ataaagaaca attaggacct tataaagaga aagcccaaaa
     4261 tcttttaaaa gatgtaaata tcgacagtgt caaaaacaaa tttaaaaatc tattcaaata
     4321 agtaaaactt tcacttttgt aaaatcaaag gaggacaatg ctgctgcatc gtcctccttt
     4381 ggttcttttc tgaatacttc atactaaaat aaacgtgtcg gatcgtaatg ataagtcaca
     4441 ttgccgttca gtgaggcttt tcccaccaca tatttacgat tctccaactt cagccacgct
     4501 tctcgaaaag ccattaattc ttctcttttt acatcaatct tttcgatttt tccatcgatc
     4561 aaatcttgaa taagttgttc atatggattc atttttttcc tccatctgct gtcaatcaat
     4621 aagttgtaaa aataggttca tatgcttttt tggtatctag atcttctctg actttgactg
     4681 tcgtataaat aattttttct actgggatac tataataact tccttcacca ttattgaatc
     4741 ggtgaacgtc tttctttgct aaaataccat ttattttatt cgtcagtaca cgttgttcga
     4801 cactgttact tacaacatag taaatcaccc gaatattatt gatcaaatgc aaatcgattt
     4861 ctaacactac atgatccatg aaaatcccac tccttctttc actttgatta tagcaagttt
     4921 ctcctctaat ttcaaaatcg atacagattt taccggataa atccacaaaa ctgcttgcgc
     4981 catttcgcac aaagtgttat tttgtatcat ggactttatt tatcggaggc aatagtatga
     5041 tcgcgatcgg tcaatttgtt ttttatattc ctttttttat tatgataagc attcttttct
     5101 attacataaa atggacaaaa aagaagtttt cagttttact ggcctcttta cctgctgtat
     5161 attttactta tcaaattttt tcttttaggc attgggaaac aacgtctgta ttaataacac
     5221 acatcatcga attgacgctt tcagtaattt ttcttattat ttggatctat tttttatata
     5281 agaatcagaa ttgaatccct cgcatgttta aaaagaaaac gattaaagat gcagcaaatt
     5341 tctgccgatc tttaatcgtt ttttctattc tgttatacca aaaaaatgtt tgaatcggtg
     5401 aaggatcgtt ccttgtttta attctataaa ttcttgtgcg atcgggtact tttcttccaa
     5461 attttctttg ttcttttcga tcaggctttt cgcttcttca tattttttat ctgcacatag
     5521 tgcttttact tcctgtaata cttttcctgc ttccatttgc ctcaccctct tgccatttta
     5581 ttgctttaag ttttatttaa gttatacgca ttattctagt ttcatcccaa ataacctttt
     5641 ttcattttta ctcctcgaac taatcagttt tctaaaggct gattggttcg tcttttttat
     5701 ttttcctcca tttcattctg ctcttctgtt aatttatcta atgctttgat tgatgctgcg
     5761 gctcttgcat aatcatagct gtataattcc ataaatttat caggcagaac aaaccacgct
     5821 ggatgataac cgttagctgt gacagggaat cctttatctt gttggttatc tccatcccat
     5881 ctcagagcaa cacctttttt tcctttccac tttactaaag cgatcgagta tcccttcttt
     5941 gtttgtgtgc cgtcttcaat aattgctaaa atttctattt tttcttttgg cgcacggact
     6001 tcttgaggac gaaccatttt ctattcctcc cttatctcat tacttcacga ccaacacact
     6061 gcaaggggca tgtgatacga cataagaagc ggtcgatcca actaatgctt gttgtatagc
     6121 accttttcct gtcgctccca taacgatcaa atcaatattt tcttgtccag ggatgacatt
     6181 ggcaatcaaa tttcttggat ctccagtttc aactattgcg tggatctcag caatgccgta
     6241 ctctttggca tcattgattt ttttcagcat ggctacttct gttctttgtt tttcatctgc
     6301 atagattttt gaaaaggcat aggcactatg ggtcagctca actttgttga tgattgacac
     6361 gataaataag tgggcttcat tgtcctttgc taagtcaagg gcttcagcga acgctttgat
     6421 cgattgtctt gatccgtcaa ctgctaccat gatgttctgg tatcttttac tcattggaac
     6481 cgcctccttt ttcttttatt gtaacatctc aagcaaaaat acaaaaaata tacgcaatat
     6541 gtaaataaaa tgatgaacaa aagcaatttc tttcaagtta tttatacatt tcttttctca
     6601 tcagaacacc tgttctaatc ccattatttc tgcattatgt gaaattcctc atcatttttc
     6661 acatcgagcg aacatcattc tgttacaaaa aacgtggatt tgtcataaat tgtcatgctt
     6721 cttttaccta tgaaatattt tcacgtgtta aagtattcct cgttgaacac aacaaataaa
     6781 actttttaac gaataaagga gatttttcat gacatcactt aaaactttac tttttggaac
     6841 aactttggct gccggcgctg cattcttcat gggaacgact gctcatgcgg acgaagctta
     6901 taccgttcaa tcaggtgata ctctatcaac gatttctcaa aaatacgttg gtgataactc
     6961 cttgatcaat gccattgcag aatcaaattc
//
LOCUS       DANJMA010000087         6973 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000087 DANJMA010000000
VERSION     DANJMA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..848
                     /locus_tag="PWU31_002532"
     CDS             <1..848
                     /locus_tag="PWU31_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1085006.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKPKIVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFVKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1056..1247
                     /locus_tag="PWU31_002533"
     CDS             1056..1247
                     /locus_tag="PWU31_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1085007.1"
                     /translation="MTKYVNEKWVSLEEAAEYLSVKPVTLRDWIRKGNGIPAFKIGKK
                     WAFKCSELDEWVKNGKSSI"
     gene            1228..2724
                     /locus_tag="PWU31_002534"
     CDS             1228..2724
                     /locus_tag="PWU31_002534"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL1085008.1"
                     /translation="MEKVLSEYEGEKLAMDLGQVFTKSSVADYMVSLLSLSKDSLILD
                     PCFGDGVFLKSLLKAGFKNVEGYEIDSKLYSKSKKKFQINQLYNKDFLFNENPTKYDG
                     IIMNPPYIRQEKIDDMSEFGITKERLKKDKLFGKLPSTANMYMYFIVKSLSMLKDGGE
                     MVVIFPNSWLTSKVGIQFEKLMYENSTLLNEIHLLGELFEKKALVEVLVLRIRKGKYQ
                     KNAIFKSYKVVEDIFVDLKKDNQSLNLGFDVEFDNIATVRRGITTGYNKMYINPGFSD
                     DSSQKHLHKIISSPKSVKGYSTVNANLDDIFIPGTDDFFSDEEIKYLNNFKTIIIESK
                     KPKTLLEKIKKNNNNWFKINSIDSIGILFNYFVRKDMKFILNESVLARDNFYIIKPKI
                     DKYLMFALLNNYYTYYQLEKNGKTYGAGLLKLQRYDIENLMFPNLDEFNEEEVEQLIM
                     ASRNLSKYGDESEIQTISKIISNHSAVEYSSIVESLDRIKELRLKGEK"
     gene            2724..3809
                     /locus_tag="PWU31_002535"
     CDS             2724..3809
                     /locus_tag="PWU31_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL1085009.1"
                     /translation="MRTKVVSLFSGGGGLDLGFQKAGYDIIWAIDNEKNAVETYKKNL
                     GDHIILGDINEFDINEIPKADIVIGGPPCQSFSLAGKRNTEDERGQLVWRYISIIKHL
                     KPKAFVFENVTGLLSAKNSSGEKIVDLLKDAFSQLDYNLELQVINAADYGIPQRRKRV
                     LIVGLRDDEVFKFPKPTHSESDDNLLPYVSVKEALDDLPESTEGINEVVEYLVSPQND
                     FQRKMRSGDTVTEHFTPTMSELDKYIISHVKPGGNYMDIPSDVPSERIRRLQRNGGHT
                     TCYGRLLPSKPSYTINTYFNRPNVGCNIHYKADRLITVREALRLQTFPDSYEIVSTSK
                     QGRNKIVGNAVPPMLSEIIAVELKKYI"
     gene            3896..6433
                     /locus_tag="PWU31_002536"
     CDS             3896..6433
                     /locus_tag="PWU31_002536"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014442.1,HMM:NF026854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BpuSI family type II restriction endonuclease"
                     /protein_id="HDL1085010.1"
                     /translation="MSFINYSDDEVKIFHPICERALNSALKNLGLDSTYEVKHHYRYQ
                     GIIPDFVILNKNTGNILIVIEVKRTPSQVMSTRYRLQAQSYITNAASQTFEQKYFALT
                     NLEKTYLFKYNPQNTSVINQVVNPSPINTGRFSDSPLIFESQLVADYTQMIQVAQTDS
                     GTYQLSYDDIVRTLCEYTDDFENWHSAITVIGYELIRSIMQNYQPSVVNVWGNAISYQ
                     RNPVVLQKLVNTIDFQSLGDGNILNDARSPYWQAEILKKASTIGNKTYSGDEFASVVH
                     ELLVKGHEGEGIVPTDMELAAALAELVSSEFSNPNEKVYCDPASGSGNLLSAIIDRNQ
                     NINPSNIWANDRLEYLKDILSIRFGLKFPRIISPSNAPKITDKDIVTLSKKDFSGVDA
                     IVMNPPYVSGVRDTVRKKMFFDKIKLLSGRKKPITDVGQMPLEGPFIELLLNLVKDGT
                     VIGIVIPEQHLFGKGRESTAIRELLLTQFGLKKIFTYPRTGLFETVTKGTVVVLGNKG
                     HSPSNVEIINSSLPIEQIDLRDLVQDTGTPFGVDRNYINVSDLSKKISSGWKSTMYPF
                     YDTFFSNISKSISTLKRSKYFRGSADNSGEGKYLYVSKQGYWNSVKSLIPHSWLKEGI
                     QNVQDYPDNGPLVHTSTTVKALCPPETAFVSGTPDNVKLLQILSIAKHYLSQQKSSGS
                     QPRQQKSLQEIANILENVTKRLVPPGRLLIPRALRNDFKVFETTAETVVSTNFIVLEL
                     TSTEKEAFLSWLMSIFGQIQIEMLSKPQEGMRKVEVTEIGMVQYPLNFSSASKPFINS
                     SGYQRSFSNYLSLNETDEYWMRELGISMSDYIGLVNLMSKLIDLRNP"
BASE COUNT         2509 a          969 c         1320 g         2175 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ctaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagccg aaaatcgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgttttcc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgtgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aatgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggagga accatttaca caaaattatt gacgctccct
      901 ttcaaaaaaa tatatataac taaataacat aaaacagatt aaaataatat ttaaaatcat
      961 ttaataataa aagatatgag gaaatgttga gaaaatgaat tatatttgct acaatactaa
     1021 gacgcaaata atattgattt tgggaggctt aaacattgac gaagtatgta aatgaaaaat
     1081 gggtgagttt agaagaagca gcggaatatt tgagtgttaa acccgttact ttaagggatt
     1141 ggatacggaa aggaaatggg atacctgcat ttaaaattgg aaaaaaatgg gcgttcaaat
     1201 gttctgagct tgatgagtgg gtaaaaaatg gaaaaagttc tatctgaata tgagggagag
     1261 aaattagcta tggatttagg gcaagttttt acaaaaagca gtgtagctga ttatatggtt
     1321 tctttattaa gcttgagtaa agattcatta attctagatc catgttttgg agatggagta
     1381 ttcttgaagt ctctgctaaa ggcaggtttt aagaatgttg aagggtatga gattgattct
     1441 aaactatata gtaaaagtaa aaaaaaattt caaatcaatc aattgtataa caaggatttt
     1501 ttgtttaatg aaaatcccac taaatatgat ggaattataa tgaatcctcc ttatattagg
     1561 caagagaaaa tagatgatat gtcggaattt ggcataacta aagagcggct aaaaaaagac
     1621 aagttatttg ggaaattgcc aagtaccgct aacatgtata tgtactttat agtaaaatca
     1681 ctatcaatgc taaaagatgg tggtgagatg gtagtgattt ttcctaatag ttggttgact
     1741 tctaaagtgg gcattcaatt tgaaaaattg atgtacgaga atagtacctt gttaaacgaa
     1801 attcatttat taggagaatt atttgaaaag aaagctcttg tggaagtttt agttttgcgc
     1861 ataagaaaag gaaaatatca gaagaatgct atatttaagt catataaggt tgtagaagat
     1921 atttttgtag atttaaaaaa agataatcaa tctcttaatc taggatttga tgttgaattc
     1981 gataatatag ctactgtgcg aagaggcatt actacaggat ataataaaat gtatattaat
     2041 cctggtttta gtgacgattc ttcccaaaag catcttcata aaataatatc ctcacccaaa
     2101 tctgttaaag gatatagcac ggttaatgct aatctagatg atatttttat tccaggaaca
     2161 gatgattttt tttcggatga agaaattaaa tatctaaata atttcaaaac aataataata
     2221 gaatctaaaa agcctaagac attattggaa aaaattaaaa aaaataataa taactggttt
     2281 aaaataaatt caattgattc tataggaata ttgttcaatt attttgtgcg aaaagatatg
     2341 aaatttattt taaatgaaag tgttcttgct cgagataatt tctatattat taaacctaag
     2401 attgataaat acttaatgtt tgctttatta aataactact atacctatta tcaattggaa
     2461 aaaaatggaa aaacatatgg tgcaggacta ttgaagttac aacgatacga tattgaaaac
     2521 ttgatgtttc ccaatctgga tgaattcaat gaggaggaag ttgaacagtt aatcatggct
     2581 tctcgaaatt tatctaaata tggggatgaa agcgagattc aaacaatatc taaaataatt
     2641 tcaaaccatt cagctgtaga atattctagt attgtagaga gtttagatcg aattaaagaa
     2701 cttcgattga aaggagaaaa ataatgagaa ctaaagtagt tagcttattt tcaggaggag
     2761 gaggccttga tttgggtttt caaaaagctg gatacgatat tatttgggca attgataatg
     2821 aaaaaaacgc tgttgaaacc tataaaaaaa atttaggtga tcatattatt cttggtgata
     2881 ttaatgagtt tgacattaac gagataccta aagcggatat tgttattgga ggtccaccat
     2941 gtcaatcatt ttctcttgct ggtaagcgta atactgaaga tgaaagaggg caattggttt
     3001 ggagatatat tagtatcatc aagcatttga aaccaaaagc ctttgtattt gaaaatgtta
     3061 cgggattact ttctgcaaag aattccagtg gagaaaaaat agttgatttg ttaaaagacg
     3121 catttagtca acttgattat aatttagaat tgcaagttat taacgcagca gattatggaa
     3181 ttcctcaaag gagaaaaaga gttctaattg ttggtttgcg agatgatgaa gtatttaagt
     3241 ttcccaaacc tacgcatagt gagtctgatg ataatctact gccatatgtt agcgttaaag
     3301 aagctttaga tgatctacct gaatctactg aaggaataaa tgaggttgtt gaatatttgg
     3361 tttctcctca aaatgatttc caaaggaaaa tgcgttctgg tgatacagtt acagaacact
     3421 tcactccaac aatgagtgaa ttggataagt acattatttc tcatgttaaa ccgggaggaa
     3481 attacatgga tattccttca gatgttcctt cggagagaat tagacgtttg caaagaaatg
     3541 gtggacatac aacttgttat gggagacttt tgccgtcaaa gccatcatat acaattaata
     3601 cttattttaa tcgtccaaat gttggatgca atatccacta taaagcggat cggcttataa
     3661 ctgttagaga agctttgaga cttcaaacgt ttccggattc atatgagatt gtttcgacaa
     3721 gtaaacaggg tagaaacaaa atagttggaa atgccgttcc tccgatgctt tcagagatta
     3781 ttgctgtgga gttaaaaaag tatatttgat tatatattaa ataaagagca cattagtgct
     3841 ctttattggt ataatttgct tataaacgtt tatgtattca gaagggagaa ggaaaatgtc
     3901 atttattaac tattcggatg atgaagtaaa aattttccat cctatatgtg aaagagcttt
     3961 gaattctgcg ttaaagaatt taggcttaga ttcaacatat gaggtaaaac atcattatcg
     4021 ttatcaggga attattcctg attttgttat tctaaataag aatactggaa acatattaat
     4081 tgtaattgaa gtaaaaagaa cgccaagtca agttatgagt acaagatatc gacttcaagc
     4141 acagtcttat atcactaatg cagcttcgca aacttttgag cagaaatatt ttgcacttac
     4201 gaatttagag aagacatatt tatttaaata taatcctcag aatacgtcgg ttattaatca
     4261 agttgtaaat cctagcccta taaatacagg tagattttca gattcaccat taatatttga
     4321 atcacaactt gttgcagact acactcaaat gattcaagtc gctcagactg attcaggtac
     4381 ttatcaacta agttatgatg atattgtcag gacgctttgt gagtatactg atgattttga
     4441 aaactggcac agtgctatta ctgtcattgg ttatgaatta atacgtagta ttatgcaaaa
     4501 ttaccaacct agcgtagtta atgtttgggg aaatgctata tcatatcaaa gaaatcctgt
     4561 agtattgcaa aagctagtta acacaataga ttttcaatct ttaggcgatg ggaatatttt
     4621 gaatgatgcg agaagccctt attggcaagc cgaaatatta aaaaaagcct ccactattgg
     4681 aaataagaca tatagtggtg atgaatttgc tagtgtggtc catgaacttt tagtcaaggg
     4741 acatgagggg gagggaatag ttccgactga tatggaatta gctgctgcgc ttgcggaact
     4801 agtttcatca gaattcagta atccaaatga aaaagtatat tgtgatccag catctggaag
     4861 tggaaattta ttatctgcaa taattgatag aaatcagaat atcaatcctt caaatatttg
     4921 ggcaaacgac agattagaat atttgaaaga cattttatct attcgttttg gattgaagtt
     4981 ccctagaatt atttcaccgt ccaacgcacc taagattact gataaagata tcgttactct
     5041 atcaaagaaa gactttagtg gtgtagatgc tattgttatg aatccgcctt acgtttcggg
     5101 agtaagagac actgtcagaa aaaaaatgtt tttcgataag ataaaattgt tgtctggacg
     5161 aaaaaaacca attactgatg ttggacaaat gcctttagaa ggcccgttta ttgagttgct
     5221 gttaaatctt gtcaaagatg gaactgtgat agggattgtt attcctgagc aacacttatt
     5281 tggaaaggga agagaatcaa cagctattcg agaactatta ttaacacaat ttggattgaa
     5341 gaaaatcttt acatatccta gaactggatt atttgagacc gttacaaaag gaactgttgt
     5401 tgtactaggt aataaggggc attctccatc aaatgttgag attattaata gttcgcttcc
     5461 tattgaacaa attgatttac gtgacttagt gcaagatact ggaacacctt ttggtgtaga
     5521 cagaaattat ataaatgtat ctgatttaag taaaaaaata tcaagtggtt ggaaatctac
     5581 tatgtatcca ttctatgata cttttttttc taatatatca aagagtattt caacgttaaa
     5641 aaggagcaaa tactttagag gatctgccga taatagtggt gaaggaaaat atctgtacgt
     5701 ttcaaagcag ggatattgga atagtgtcaa gagtttgatt ccacattctt ggttaaaaga
     5761 aggaatacaa aatgttcaag attaccctga taatggacct ttagtgcaca cctccactac
     5821 tgttaaagct ctatgtccgc cagagactgc ttttgtgtct ggaacacctg ataatgtaaa
     5881 attattacaa atattatcta ttgcaaaaca ttatttaagt caacaaaaat caagtggttc
     5941 acaacctaga cagcaaaaat cactacaaga aatagccaat attttggaga atgtaactaa
     6001 aagacttgtg cctccaggaa gactattaat accacgtgct cttaggaatg attttaaagt
     6061 atttgaaacc acagctgaga cagttgttag tactaatttt attgtattag agttaacttc
     6121 gactgagaaa gaggcctttt tgtcgtggct aatgtctatt tttggtcaaa tacaaataga
     6181 gatgctctcc aaacctcaag aaggcatgag aaaagttgaa gttacagaga ttggaatggt
     6241 tcaataccct ctcaactttt cttcagcctc aaaacctttt ataaattcta gtggttatca
     6301 acgatctttt tctaattatt tatcattaaa tgaaaccgat gaatattgga tgcgtgaatt
     6361 aggaataagt atgagtgatt atattggatt agttaaccta atgtctaaat tgattgactt
     6421 gagaaatcca tgagtaaaaa aagctgccaa catctaaagt tgccagcttt tttgtaccaa
     6481 tacccattat tgcttcctca gaaacaacaa tgggtattgg tacagtagca taattataca
     6541 atcaaattta aggataatat taacttttag aactaatagc acagaagagc attcttatca
     6601 aagtagtaaa acatatgaat ttaaatcaga aaatatcatt cactcatgat acccagctcc
     6661 gtatcacaaa taatgatatt ttttaagaga aaaaattatc cctattttaa ataatagcaa
     6721 taggtagtgg tagagagaaa tttataaata actttgttat ttgctttggt agccaagtaa
     6781 gttgcaaaag aaaaatagat actgttaaaa agataatgta gacatagaaa caaatacgta
     6841 aaattttagt tagaatgatg gcaaggagaa aaaacacgat agatgaaaaa aagtgtttaa
     6901 aaaatctatt ggaggatatg gatgatattg aagaaaaact atctaaatgg actgggaaaa
     6961 caaatatatt tca
//
LOCUS       DANJMA010000088         6676 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000088 DANJMA010000000
VERSION     DANJMA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(164..778)
                     /locus_tag="PWU31_002537"
     CDS             complement(164..778)
                     /locus_tag="PWU31_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1085011.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            922..1484
                     /locus_tag="PWU31_002538"
                     /pseudo
     CDS             922..1484
                     /locus_tag="PWU31_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="PWU31_002539"
     CDS             complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="PWU31_002539"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL1085012.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(2588..3043)
                     /locus_tag="PWU31_002540"
     CDS             complement(2588..3043)
                     /locus_tag="PWU31_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1085013.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(3071..4516)
                     /locus_tag="PWU31_002541"
     CDS             complement(3071..4516)
                     /locus_tag="PWU31_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1085014.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="PWU31_002542"
     CDS             complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="PWU31_002542"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL1085015.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            5616..6374
                     /locus_tag="PWU31_002543"
     CDS             5616..6374
                     /locus_tag="PWU31_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1085016.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         2069 a         1412 c         1082 g         2113 t
ORIGIN      
        1 tacaaaaata tttttattgt tggatacaga aatctatttt taactgggga tatctttgcc
       61 agctaatatc tttgagacta cacctatctt catctaaatg gatcaattac tcgctttttc
      121 cattgacctt cgtggtactt ctgctttctg atagtaaaaa acattactga atgatcttac
      181 tatataacat acagcggaga tctacttctt tattaaggat cttcatgacg ttatcttttt
      241 cttttttccg ttcgaaaccg cgtcgttcat aaaattgata cgtacagccg ctatccgtga
      301 acagaaatac ttcttttcct tgctctcggc gttcaaattc ttttaataat ttactaccga
      361 tccccttgat ctttaaatca gggttggcag ctaaaaagac aatttctcca tctggggaat
      421 gtttttttaa atagtctgaa agcaattcgt cattggtgtc gtcatacccg cctttgctgt
      481 ctttcacaaa tgcattttgt aagaaatcaa atagtttaac atataacttt tgactaaatg
      541 aatgatgctt ttttgatttt cctttgatgt ctgctaataa aacacctgcc aactcatcat
      601 ccgcatataa agcaatgatt tgtgtcgcgc gattcaattc tagataccaa aagtatcttc
      661 cataaagatt caaaaggagt ttgttcttgg tataccaatc aaagtgcata cctttaatcg
      721 cgaattgaat ggcttttttg tagtctttct tttccagttg cttaaaggct aattccatca
      781 cttattctcc tattctttaa atattgatat gctatactta tattataata gacaaggtgt
      841 cggttatcta tgttcagata gcggtgaaat tgtcggataa ccgacacaat tccttgatta
      901 tgttgattag gaggctgcaa aatgaaaaaa gaggatcgcc gcgtcaaaaa aacagaaaaa
      961 gcgttagcag aagcattatc aaaattatta gtagataaaa aaattcaaaa catcaccatc
     1021 cgtgaactga ccgaaactgc tgatcttcac cgttcgacat tttatacaca ttatacggat
     1081 atctatgatc tttatgaaaa actggaaaat agctttttcc aagacttgga tgaaatcatt
     1141 tcctacgata cgacacatag ctacgaggat ctatacgctc aattgatcga ctatgtctat
     1201 acgaatacgt tcgtatacaa gttattcgtc atcaaccacg gagatatcca atttcaaaag
     1261 agattaaata atctgatcga agaaaattat ttaaaaatct ggctacaaga agacaaaaaa
     1321 aaccgtgatc acagaagaaa tgcgttactt caccatctac catatccaag ggtgcctctc
     1381 cgttttaaat cattgggtag aaaagcaatt ttgcatactc taagaaaaaa atgctgacct
     1441 tcttgcgctt attgaacgac aacatagaac ctatcatgcc ttaaaaacaa acgaaaaagg
     1501 aagcgtcggg acgcttccaa ttctttattt aaaagctcaa attgattatt aaaaatcatt
     1561 aggttacaaa cttaaggtta tagttaaagc tttcttacaa tttaatagct gttgctgttt
     1621 ctttcaaaac attattcagc tcttcgatgt tcttgcgtcc ttgtgtctgc atccattctt
     1681 tggcagcata ttcaccttta gtaacgaatg gttctacacc attcgcccaa gttgcgcgcc
     1741 cacagagtac cccattgaat gtagagccag cctcttttgc aaatttcaag gtttcttgga
     1801 acaattcagc agttactcct gcgctcaaga aaataaatgg caggttggta gtctcggatt
     1861 gttctttgaa ataagcagca gcttcttctt tggtataagc aacttcattt ttagtgtaac
     1921 catccacata gttcatatta acaggaactt ccactttcaa aacgtcaact ccatagcgag
     1981 gcttagaaaa ttccttcatc atttcattta ctttatgcgg ttttttcaag gcatattcct
     2041 tcgaagaagt atcaggatcg ttggcatcat aagaaaccaa ttctaagaaa aatggcaaat
     2101 cttcagcaac acattcagaa ccaatccgct ccataaacgc cttcttttgg tcattgatgt
     2161 catcagattc atctacatcg tagtataaca agaacttaca agcatcggcc ccttgctcct
     2221 ttagacgttt tacagaccaa atagataaaa tatccggtaa acgtcctgga gttgaagcat
     2281 cgtaaccagt tttttcatag gccaacaaca gaccagcatt tttatcgcga acttcggctg
     2341 ccggcaaccc atattctgga tctaataaga ttgctgaagc atatggagtc aattcagaag
     2401 aaatgatttt tttgaaattg atgatttgtt catcggttgc tgatttattt tgaaatttac
     2461 cgatcatttt tttcaaggca ccacgttgat cgattgctaa cgcactgatg atgccacttt
     2521 cattacttag ttgacgaata taagttttct ttttttctgt taaaggcatg atttcactac
     2581 ttccttttta gttttcttct aatttttggt ttaaaagttc agctatttct tcagcagact
     2641 gggcttgttt cagaccttct gtcacatctt tgtgcatcaa aagtctggct acctgagata
     2701 aaaattttag atgggttgtt ccagcctctg aatctggaat aaacagagcg attacaaaat
     2761 ccgttccttc tccatctaaa cttttccaat cgaccttacc gtttaaccga acaatcacaa
     2821 tatcggcatc cttgatacta gcatctttgg catgagggat ggcaaaacca tccatcatac
     2881 cagtcgtgct ttcttcttct ctttccatca atttttcgta aactgcatct cggtcttcac
     2941 taatgctcaa gacaattgac tgatctgcaa ggaagttcat tacctcatct tgcgtcgtaa
     3001 aatttttatt cataaaaact ttatttgttt tgatcaaagt catttgaaat ccttctttct
     3061 agaataacct ttagttgttg gttgcttctt tcatttcaac agcagctgct tctgcttcac
     3121 gtttttctac actacgtttt tggatcaacc ctaaaattac tccgccaacg attgaaccaa
     3181 gtaaaatcga ccctacccat gcaaccttac cagtaacaac aggtaatact agaaaacctc
     3241 catgaggtgc gggaacttgg acaccaaaca tatatgtcag tacagcagca attgatgaac
     3301 ctagcatcat agttggcaga acacgcagcg gatctttagc cgcaaatgga attgctcctt
     3361 cggtaatgtg cgttgagccc aagataaagt tgactaaacc agcgttgcga tcattcgtgt
     3421 cgaaatattt tccaaaaaac agtgcggcaa atccagtaat caatgggggc gcgatacaag
     3481 ctgccgaaac ccctgccata aatgtatggt taccttgtgc caacaacgca gtccctgtaa
     3541 cataagccgc tttattgact ggacctccca tatcaaatgc tgacatacag ccaacgataa
     3601 tccctaaaat taatggatta gaattttgga atcctgccaa gaaattcatc attccttggt
     3661 tgactgcttc cattggattt gctaataaag acatgactac accaacaaac aagacaccga
     3721 tcaccggata taagaagatc gccttcagac catccaattg tttaggcaag cctttcatga
     3781 ccttttgcaa tagtaagata aagtagcctg ctaagaaacc tgcaaaaatt ccgcctaaga
     3841 aaccggctcc gccagaattg gctaacagtc cgccaacaaa cccaacaacc aaaccaggac
     3901 gtttagcgat tccttcagcg atataagctg ctaaaatagg aaccatcatt cccattgccg
     3961 catcaccaac accctttaac atagcggcag tcggattgta agtaggatca ttgggatcaa
     4021 aagagtacac accccagatg gcaaacgaaa ttgcaataat taccccacct gccactacaa
     4081 acggcagcat atgagaaaca ccgttcatga gatttttata aatactgtga ccaatgcctg
     4141 ctgcaacccc catctccgct ttctcaccag cagcgtcaac tgttttgttt tctgcagtga
     4201 ttgaaccttt accagccaac gcatcttcga tcaattggtc agcatcttta atccccttgc
     4261 tgacaggcac atcgattaca cgcttacctg cgaaacgttc tgcttgaaca tctttgtcag
     4321 ctgcaatgat tacagcgtca gcgtttttta tttcttctgg tgttaattca ttctctatcc
     4381 ctacttgacc atgggtttca actttgatag ataccccttt gcgcttagct gcctgctcca
     4441 atgcttcttg agccatataa gtgtgggcaa ttcctgttgg acatcctgtt gcggcaacaa
     4501 ttttgtaagt tgtcattttt tactcgctcc ctctttgatt tcgatttgtt tttccaactc
     4561 tgcaacatcc gaaaaatctg ttaatccttt acgaaaagcg gttgaacttc cagctgctaa
     4621 acttttcttc aaattaatat ctacctgttc accttttaaa tatcctgcaa gaaatgttcc
     4681 caacaaagtg tcaccagcac acgctgtatt gactacttta cctttaggag aattcccaga
     4741 ataaacgata tccttattta aaaatattcc tccctctcct cctagagaaa ggagaacatt
     4801 tttcgctcca tcggcaatca agcgttcagc ataatgaata tatccttcac gatcactaag
     4861 ctcgacatta taccaagatt gcaattcctc ctcgtttggt tttaataaga acggctgata
     4921 cggcagacaa tcaattattt ccggatagct tgaatcaata atcagatgga ccttattttc
     4981 aaaacaaata cgactgatat caataagaat ttcgggtgag acccctcgcg gcaaactgcc
     5041 agaaacacac aaataatcgt cagcttgcag cttttgtatt tgatttagaa aagttttgat
     5101 ttgttcctga ggaatctcag gtcctttatt aaccaatttg aattcttcat tggttttatt
     5161 aacttgtgta aaaacgttga ttctagtcat tcccggtacg ctaataaaat cagtgctaat
     5221 atctttttga tgcaaataat cctcaatata attcccggta aaacctgctt taaatcctaa
     5281 agctgtacta tcgataccta attgcttcat gatcaatgaa acattcacac cttttccatt
     5341 agcttgtata tcatcatcgt ttgttcgatt gacttgggat ggtttcattt catctgtttc
     5401 gataaaaagg tcgattgcca agtttaaagt acatgtataa actgacattt acgctcctcc
     5461 tttcttacat attcattatg tatcaagatc gaatgtaaat gttttcataa atgactaaat
     5521 tcattgaaaa cttttacaaa atatattaaa taaacttgaa gtaaaggaaa ctcccgcttt
     5581 acaatagaat tatcctaact aatcggaggc aaaatatgga tacaaaatta agtgaatcgg
     5641 agaaatatct ctgggatttt attcaaagtc acatactcga aatcccctcc tattccattg
     5701 taaaactaag tgaatatgcc aacgtttcaa cagcaaccgt cgttcgtact atgaagaaaa
     5761 aaggatatga ggggtttacg tcttttaaac attcgttaaa agaaaaagaa aatacaaata
     5821 tcaattttgc cgctcttgat aaagttgatg aaggaataaa gactgctatt cttaaaaatg
     5881 agcaagaggt aatccgcacc atcaatatgg tagaaagcgg gaatattgaa gatgctattc
     5941 aacgaatcaa ggctgctaga agaatcatga tttttgcccg aggtttttca gaattgattg
     6001 ctcaggaaat gatggtcaaa tttcaactta ccggtaaata ttgtgagatg catactgatc
     6061 ccaatatcat aaaaaacatt agtagaaaat tatccaaaga tgacatcgtg atctttgttt
     6121 cactaaacgg tgaaacaaag gaattgacta ccgcagctga aaactgttat aaagctgaga
     6181 tcgggacaat tttgcttact gctaatcgtc attcaagttt gatggaacat atcgagattc
     6241 cttttgttgg ctttaaatca gaaggctcct tcttcccaga ttatgaggta cgctccagac
     6301 ttccactttc tatactggct agaatcttac ttgactccta tgcactgcga acatcaggat
     6361 tcacacagca ctaactctat atctgtaaga ctgtacctaa aatatatctg tgtgaattaa
     6421 ctaaggttgt ctagaaaaat atttaatcgc tatttgtatt ctcgtgcttt ctatcattgc
     6481 tccttctata acttcttagt ttcaagatag aatagtgcat tgagactacc aggactactt
     6541 acgcaaatat ataataataa gaatgtgata agcagttcga agttcctgtc taatatttct
     6601 tgttaattat tatcaatttc caacaattct agaattataa tacgcaaaaa aaggagaccg
     6661 tctaaccgtc tcctta
//
LOCUS       DANJMA010000089         6643 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000089 DANJMA010000000
VERSION     DANJMA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(1..859)
                     /locus_tag="PWU31_002544"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="PWU31_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="PWU31_002545"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="PWU31_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL1085017.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="PWU31_002546"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="PWU31_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="PWU31_002547"
     CDS             complement(2603..3421)
                     /locus_tag="PWU31_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1085018.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="PWU31_002548"
     CDS             3582..4271
                     /locus_tag="PWU31_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1085019.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="PWU31_002549"
     CDS             4285..5787
                     /locus_tag="PWU31_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1085020.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="PWU31_002550"
     CDS             5800..6276
                     /locus_tag="PWU31_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1085021.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="PWU31_002551"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="PWU31_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DANJMA010000090         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000090 DANJMA010000000
VERSION     DANJMA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            725..811
                     /locus_tag="PWU31_002552"
     CDS             725..811
                     /locus_tag="PWU31_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1085022.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWU31_002553"
     CDS             complement(932..1621)
                     /locus_tag="PWU31_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085023.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWU31_002554"
     CDS             complement(1628..1906)
                     /locus_tag="PWU31_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085024.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWU31_002555"
     CDS             complement(1866..2780)
                     /locus_tag="PWU31_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1085025.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWU31_002556"
     CDS             complement(2762..3142)
                     /locus_tag="PWU31_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL1085026.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWU31_002557"
     CDS             complement(3592..3879)
                     /locus_tag="PWU31_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1085027.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWU31_002558"
     CDS             complement(3899..4123)
                     /locus_tag="PWU31_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL1085028.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWU31_002559"
     CDS             complement(4578..4748)
                     /locus_tag="PWU31_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085029.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWU31_002560"
     CDS             complement(4763..5296)
                     /locus_tag="PWU31_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1085030.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWU31_002561"
     CDS             complement(5317..6159)
                     /locus_tag="PWU31_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1085031.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANJMA010000091         6038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000091 DANJMA010000000
VERSION     DANJMA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(15..341)
                     /locus_tag="PWU31_002562"
     CDS             complement(15..341)
                     /locus_tag="PWU31_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085032.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(328..1131)
                     /locus_tag="PWU31_002563"
     CDS             complement(328..1131)
                     /locus_tag="PWU31_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1085033.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(1484..1774)
                     /locus_tag="PWU31_002564"
     CDS             complement(1484..1774)
                     /locus_tag="PWU31_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL1085034.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(1910..2089)
                     /locus_tag="PWU31_002565"
     CDS             complement(1910..2089)
                     /locus_tag="PWU31_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085035.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(2086..2436)
                     /locus_tag="PWU31_002566"
     CDS             complement(2086..2436)
                     /locus_tag="PWU31_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085036.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2429..3754)
                     /locus_tag="PWU31_002567"
     CDS             complement(2429..3754)
                     /locus_tag="PWU31_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL1085037.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            4204..4818
                     /locus_tag="PWU31_002568"
     CDS             4204..4818
                     /locus_tag="PWU31_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085038.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(4983..5987)
                     /locus_tag="PWU31_002569"
     CDS             complement(4983..5987)
                     /locus_tag="PWU31_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085039.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
BASE COUNT         1857 a         1102 c          884 g         2195 t
ORIGIN      
        1 atattgaatg taaattaaat tttattttgt ttctttgcta gtttatatcc ttcaatcaag
       61 tattcttcag gtatggactt taatagcgca tcttctataa cgtctggttt acttttgtct
      121 gattcagcaa tagtcgtcca atatttgagt gctgacatta gaattggatt tatacgaatg
      181 ctatctgctg ttcttacact acttccttga tcttttatga ctttacttat atttttcttg
      241 gttaattcga tttcttctac ttcttttgat ttaaaattct ttttattttt atttcctagt
      301 aatctactta ctgcagcttg actgcgatta tcttctgcca tcaaaatcac caacttcttt
      361 taaatattct tctgtaaatg ctttatagtc atcttcagct gttgagggtt tattaaaatt
      421 gattagatct gatccagcag cttgcgcttc gtcaacggtt attgtcctac gaatataagt
      481 tttgaaaatt tttgcaccaa tgactctaga aatttcggtt gctgcagacg tcatttcttt
      541 agaaatgtta taacgcttat tatacttagt aaatagaact cctcgtactt caagcttgtc
      601 gttcgtatat tcttttaccg tttcaatagt ttcacttaac tttattagtc cttttaatga
      661 gaaatacgca gcttcaactg gaatgataat ttcatcagaa acagtaaatg cgttgactgt
      721 aagcattcct aaacttggtg gacaatcaat tataatataa tcatagttgt ccttgattgg
      781 ttgaagtact cttttaagcc tgtgttcact tcctacttga gtaaattccc tctctgcaga
      841 agaaagggct atgtcggctg gcaaaacatc aattccttta tagtccagaa tagcatctgt
      901 aatgtcagcc tcccctttta atacttcata aattgtaggt tggtttgttc catctatatc
      961 gaaacctaca ttatctgtag catttccctg aggatcaatg tccactaaaa gtacattttt
     1021 accgtaaaca aatttcaaac atgctgctaa attaacgcta gtcgtagttt tagaaactcc
     1081 acctttttga ttggctactg acaatattct tgccattaaa atatccccca ttcatcttat
     1141 attataatat aattatatca taatattgtg atattgcaat atcgtatatg cattatattg
     1201 taatattgtg tgattatttt gttttaaaga tatatatcgg ttcggatata aagattattc
     1261 atatgttatt cgaaattaaa ataatcactt tttattactt taaaattttt tattaatgat
     1321 aagaaatatg ttttgcgagc agcaactaga ataatttaaa cattactaaa ctgtctatat
     1381 tgttactaat aattcccttc ttatagtgca ttttcttaga aaaaaacaat attacgatat
     1441 catgatatta agatatcgta atattataca attgtgattg atattatata tgttttttta
     1501 tttttttatc atgtttaatc ttgaaggaat catattcttt ttctaaagac aggagctttt
     1561 cataaacatc agacatgttt aaactagagg ccaggcttaa atcataaacg aatgaagcag
     1621 gtaatgattt tattctacgt tgtcgggtta tccagcttgc aaccgttcct tgtgggtagg
     1681 catataactc acaaaattgt tctacagtga ttcctaaatt cttgataatg taaacattga
     1741 tcgggtgcga gtaaacaaat gtattgatag acatggtgcg taagcctact ttcttttatt
     1801 attgcgtttg caataatagt ttaacacacg cattattcta gtatctactc ctatttgtaa
     1861 aaacaaaaaa ggattcatag agataactct atagaatccg taaaatacct tatatatata
     1921 acgggaagat ataaaaccta aaattatgat aacctgctgc agcttctaca tagaaagaga
     1981 agcgctcaac accaacctca tttatcaaac gttggccata accccaaatc tcttctgata
     2041 gctgatcaaa ggttatatac tttagctcat attgctggat aatgttcatt ctaatcgact
     2101 ccatttatta tcctggtaaa attcgatatg acgtatttcg tcgtagtgaa ttttgtcttt
     2161 tccaacgtag atccccagct catcactacc taagatcttg ccaatgatat cttctggata
     2221 gtatccttca gcgtttcgtt cttctttttg aatagatact gatctatttt ttatgactgc
     2281 agcgttcaat acttccgcaa tctcttcagt cgtcatttgt tcttttgcta aattttgact
     2341 ggttcgttgt tcattgtcgg catcaatttc ttcagtatga tcagataaat aaaaccctgc
     2401 ccactttaaa cgtccacgat cattgtaatc aatcattttt aattccccca tttccgcccg
     2461 catgaccacc aacaagtgtg cttctagtaa tgcttctagc gccttctaac attgaagaag
     2521 catgtacaat cgacgtaaaa ccatattttt gccgaatctt gtcaataatt ttatctaaat
     2581 tttcttcgtc aatttgcttt tgtggatcac taaataaatc cagctgaagc gaatcggtgt
     2641 acacaagctt gctataggtt attccaacat gccttacttc ttgtccttca taatacttat
     2701 caaacaagaa aagacaataa ttctgcaatt cctttgtatt atctgtgata gggatcttca
     2761 tctggtgtga aaaccctggt ctcgtttcta agatcgacgt gccaatgtgt aaatggacac
     2821 aacctgtttt acagttatgc tgcctaatgc gtatcgctac ttgttccgcc atttctttta
     2881 ccaccagctc gatttcattt cttctagtat aatctcgtgg taacacttgt gaatttccat
     2941 atgatttttc ctttgtcggt tctggtggga ttgctatatc tgttcgatcg atcccattag
     3001 catggaagta cagctgtaag ccaatcacac ctagtctatt cttaatcgta taaggattcc
     3061 agttagcaag atctctaatg ctcataatcc ccatctgatt gagtcttttt ttcattcgag
     3121 agccaattcc ccaaaagtcc gtcatttcag gaatattcca tactttttca ggaacgtttt
     3181 cgtacgtcca ttccgcaaca aatccttcat tatgctttgc ttcgttgtct aaggctagtt
     3241 tagccaaaag gggattatct ccgacaccta ctgtagcaat caatcctagc tcttctttaa
     3301 tattctcctg gatcatttga gccagcttct ttctccgttg acttcgtgtt ccttcagtcg
     3361 taaaaagatt cagtgatcgg gtcactttta agattgattc atcgatcgag taaatcagta
     3421 gatcttcatc agccacatat cttctgaaaa tattatttac ctgcatattt cgcttgatat
     3481 ataggttcat acgtggtgga acaacgtgta gtgttttagg aaatggttgt ggtaagtcac
     3541 gtggtctact cacatttgta ataccatacc gcttttttgc ttcaggagaa gaagctaata
     3601 tcaatcctga accagtatta tcagctcgac tcataacaac aagttctgtt gttaatggat
     3661 ctaaatttct ttctatacat tcgacacttg cgtaaaagct tttcatatcg attaaaaagt
     3721 aatcatttac tggctctttt gaataatcca tcataaataa cactccattc tttcacatta
     3781 tacaaacata tgttcgatat tgtaaagaag aaatagctaa atacataaaa aaagaagcat
     3841 aggtatgcta tcaccctgct tcttcttttg atttgtatga tcaatttatt ctatcacttt
     3901 actaattaat ttgaaaatct aataaaaact ctcactctaa aagagtgaga gactgcaaat
     3961 tagagtacgg acttttttgg agttccgttc tttaatatta acaaatgaat aaaatcctta
     4021 caacatttga gaataagtta tcagtagaca cttacaaaaa attctcatac tatttcctcc
     4081 atcattagtt tttgtctata taaggtttac cctaaataga cactggtata acattgaaaa
     4141 atatggcttc ctcgtgtatg attagggtgt attttaaata gttgcagaaa gtaggtaaaa
     4201 gttttgagag ttgcatacat acgtgtctca tcgatcgatc aaaatgaaca acgccagatt
     4261 gaagaaatga aaaaattcgg tgcagagaga attttcatcg agaaacaatc tggcgcaacg
     4321 attactcatc gacctgtttt ccaagaagca ttagattacg tccgagatca agatattttt
     4381 attgtggaag ctattgatcg acttggaaga aattatgacg agatcatcgc ttcagtcaat
     4441 tacttgaaga agaaaaatgt gaagctgatc attactagtt taccgatcat ggctgaagca
     4501 attggcaatc ctctcctgga taaattcatt aaggatctga tcatccaaat tttggccatg
     4561 atcgctgaac aagaacggac tgaatcgaaa cgtcgccagg cacaaggaat taagatcgca
     4621 aaagcaaacg gcgtctacaa agggcggccc aaattataca gtgcagacgc aaaagatccc
     4681 caacgtcgcc ttgtttacaa aagcatcgtt gaagatctta aaaatggtgt tgcgattgct
     4741 aagattgcta aagactacaa tgttacgaga caaaccgtgt atagaataaa aaaagacagc
     4801 atggtcaatg acaaatagtt gatcatgctg cttttattat cttctcatct ttaatttcct
     4861 acatataatt actatctata aaattaagtc ttactcagaa cagataaagc ataaaattcc
     4921 aacaaaccat tttttactta actaaataaa gagtctgtta atataatctt cacttattta
     4981 cgttaatccc ctactgtatt tgttggttcc ttatctatat tttctacaaa atccttatta
     5041 tcttgtgatt tagcttcgta taattctttg tatgtcgaca cattggttat actaattcct
     5101 atagctagtt cttgagactc tacttttctc tctagctcct ttgacaaagt gatttgagat
     5161 ttatttggtt ccgttggagt caattctata gattcttcat aaaaatctct atacataaag
     5221 ctatcattaa ttttataaaa aaaattaaat gtttttagca ctgccatata acattcaaaa
     5281 cattcttcta tagttggttt acttccttga ggagaatgaa ccaatttatt acgtttttct
     5341 atgaaatttt taatttcctt tcttatttct ttcggaaact caatattaaa gtacccttta
     5401 aacatgctcc ctaataattt gtctacaggg ggagattgta aattctcaaa tattagagtt
     5461 aaatcaggtt tctcttgttg ataaaattcc tttaccccta tttcacaggc tgtaattatc
     5521 gaaataaacc tatctctagt atttgatata tttttacttc ttttaaggag tataagagaa
     5581 aaggggatac tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc agaagttata
     5641 tctctaatta tatagttaac ggaattagga agtgtcttta actctaatga aatttctatc
     5701 caattactat cttctaatct gtatatgctc gaagtataat ttgattcgtc agcaagcatc
     5761 gctacatccc aaacactgat cataaaatct tttaaacgtt caatcatttc agcaatattg
     5821 ctaaaatcag ctaatttttc tttagaataa aaaaaaacat ttatatcaga atcaataaat
     5881 tctatttttt ttattactat atcgtcatca ttatcttgac catttatgtt tattgttccc
     5941 tgagtgggga gtacttcttt caaattcaag ttaaatttat attccattac ttctcctcca
     6001 atatataaaa attacttaac tgtatccaaa tttaatat
//
LOCUS       DANJMA010000092         5703 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000092 DANJMA010000000
VERSION     DANJMA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            300..629
                     /locus_tag="PWU31_002570"
     CDS             300..629
                     /locus_tag="PWU31_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085040.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(916..1401)
                     /gene="vanZ"
                     /locus_tag="PWU31_002571"
     CDS             complement(916..1401)
                     /gene="vanZ"
                     /locus_tag="PWU31_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL1085041.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNEHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1554..2465)
                     /gene="vanY"
                     /locus_tag="PWU31_002572"
     CDS             complement(1554..2465)
                     /gene="vanY"
                     /locus_tag="PWU31_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL1085042.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2893..3501)
                     /locus_tag="PWU31_002573"
     CDS             complement(2893..3501)
                     /locus_tag="PWU31_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL1085043.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(3507..4538)
                     /gene="vanA"
                     /locus_tag="PWU31_002574"
     CDS             complement(3507..4538)
                     /gene="vanA"
                     /locus_tag="PWU31_002574"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL1085044.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(4531..5499)
                     /locus_tag="PWU31_002575"
     CDS             complement(4531..5499)
                     /locus_tag="PWU31_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL1085045.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1691 a         1212 c          974 g         1826 t
ORIGIN      
        1 ttcctccatt attttacatt tcgtaattac ttagtaatta cattgtacgc tatttttagt
       61 agaaatacaa aataataaaa ctcctttaga acaattttca aaggagtttt atttgattag
      121 gaaattatgt agaataataa tatgttttta acttggaatc aaaaacgcac ttgcaagtaa
      181 gtttacttca atacatgaaa ttgcttataa tagtgtattg gaattaaggt agattgacaa
      241 aagagtgatc aaataggtat acttcattca aataatcgtt tgaatgtgag gtgagatgaa
      301 tgaaaaaagt ttgccaagtg actgtcgtac ataaagaaaa agtaaatgga ataaaaaata
      361 aattggatca aaaagatttt tcaaacttac ttgtgttagg gaaatgtttc agcgattttt
      421 ctagaattaa aattttctat gctttagaaa cctataagga aatgtgcgtc tgtgatttag
      481 cagaaatact tactgctagt gtcgctacaa cgtcacacca tttacgtttt ttaaaaaaac
      541 atggaatggc aaaatcacgt caagatggaa aagtagttta ttactcttta gcgaatgaag
      601 acatcctttc cgctattcgt gttttttaaa gttatcagaa aatctatcta tgaaatctta
      661 gtttgggggt accgtcagga aaatgcggat ttacaacgtt aaggaaattt attaaagaga
      721 ccaattcctc agcactaaaa ttgataattg ggctttcttt gttactatag tgttaggtaa
      781 gctttttact ttatcaccgg ttttcccctc acttcacacc tacgggcgag tttccccaca
      841 tcccgttttc catctaatcc aattcattca atatctttat gtgtatgaaa gatcagattg
      901 cttgatttag acatcttaca cgtaatttat tcttaaatgg gtacggtaaa cgagcaataa
      961 tacgagcaaa agtataccta caaaaataat aactctgtct aatttttttt gattcatatg
     1021 ctcattgctt aaaccatata atttcagtcc aagaaagcct ccaacagtat ttgtaattac
     1081 atctgttatg tctgtcgctc caatagcgaa gataaattga attatttcaa aagtaagact
     1141 taaaaccagt acaaaagcaa acttaggtaa aaatccgatt tctttaaaat tgacattcaa
     1201 aagcaagcca aatggaataa agattataac attatctatc atctctctga aattcccagt
     1261 agcagtaaat ggagtcaagt taagacttct ttgatgataa ttaaatactg ataaaatatt
     1321 gtattgtaat ttgaataaca ctaaccagat tagtgtcact aaatataaag ctagcaatcc
     1381 tctagataat atttttccca atataatttc ctctaattct tttgtttaag ttgtatcatt
     1441 agtatagcaa tgtcctagtt aacgtgggtt ttaaattaaa aagctaacgt taccccaaaa
     1501 gtgctagtac aataatgctg ttcattaatt tcgttggttt cattccgcca tccttacctc
     1561 cttgaattag tatgtgttga tccgggaaac actgtcacaa ttacaccgtc tatattgttt
     1621 cctgatatct cataacgaag attagtcggc acatgaatgg tggtattttt agtaacagga
     1681 taataaaaga tctcatattt ttccccattt acactaacag aaatggtttt ttcttctttt
     1741 aggtaatcca tatattcctc gagaacgaaa ttcttttctt tcataatcgc actatgtggt
     1801 aaaccaacat agcgaatatg ccatagttca tattgaattc ctgttaactc tgttttgtcc
     1861 tctggataac gtaaaatgaa cccgtatttc caagcatttt cttctatcca ctttccttca
     1921 ggggctcgtt ccattttcgt caagcttgat cctacatcta gtgataaacc tgaattatgc
     1981 tcactataac ctgctggtaa ggcatactca gcccccattt cttggtaaag cacactttgc
     2041 tcatcaaagt ctcgatagcc actattaata ataaaatgac taacgccacc ctttacagca
     2101 tcattgacca tctctgaaaa tttttgtgct atttcttttg acatataaat attactatca
     2161 agcaacccgt atccatttat taattcgtca tgtttagata aattcacgat atctgacttc
     2221 acactttctt ggcgaacagg atatttacta ttgattaata gcagatttcc ttgataaacc
     2281 tgttcttctg taattgtttt ctcttgggta ttttcagaag tcccactatt ttctaaatgt
     2341 tctttgggat tttgatcata attttgaaat tcgacttttt cctgagaaaa cagtgcttca
     2401 ttaacgtagt cataacctaa gtatattaag aataacaata acaataaaaa aaacaacttc
     2461 ttcattttca gtctcctcct taactatata gttggaagta tgaaatattt ctagaatctt
     2521 ccccttaaac ggcacaatct cgtttgagat tgtgccgttt tgccccgtaa attacagtca
     2581 attatttcgc cctactggat ttactattat caccaatgta gatgaatttt cataagtgat
     2641 acccatgctg aatttaacga ggctgaactg cctgttgaca atgggcggtg tgaaatacca
     2701 cccctctatg cgatcagtct tattatcagg ctatcttgcc gcagcgcacg cagataatga
     2761 ccgcaatatc gctctgcctt acatcttcaa tactatctac tatatataaa ttccctaaga
     2821 accagttaca tacgtcgggt ttcctgccga aagagttagc ttatatagtt tgtccgtgca
     2881 acggttaaaa gtttatttaa cggggaaatc aaaatagcta ttggggtatg gttcgtctct
     2941 taatacatag tgccaccatt cgaggctata tgcttcaaac ccactgtttt ccatgatgga
     3001 gcgcaaacgt ctgcgatttt gcgcttcatt gcatgatatt ccatttgccg catgatgaga
     3061 gcgttcatcc ataaaatcaa atcggctccc cattggtaca agctcacccg tgtctaatcg
     3121 ataaagcgta agatcaatgg cactgccgcg gctatggctt gattttgaag ccacgtatcc
     3181 ttttgaaatc atctcagttc ggtcaatatt gggataataa ctttcctttg tcaggttatt
     3241 ttccggctgt gcagcccatt gcataaaaca gtttacagca cgattaggac ggtaaccgtc
     3301 ccatagaagc aatccgtacc cttgggtagc agccagttct tttgccttca aaagcgattc
     3361 agccaactcg tatgtcccta caatgcgatt tacttcataa ccgtcaaccg gttttccggt
     3421 gaaattatcc caagtggcat atttagcgtc ccaacgaaca ccgtgtacta tttcatctaa
     3481 aaaagtaaat cctatttcca tgcttatcac ccctttaacg ctaatacgat caagcggtca
     3541 atcagttcgg gaagtgcaat acctgcagcg gccatcatac ggggataacg actgtatgac
     3601 gtgaaaccgg gcagagtatt gacttcgttc agtacaatgc ggccgttatc ttgtaaaaac
     3661 atatccacac gggctagacc tctacagccg agcgctttat atattttttt tgccgtttcc
     3721 tgtatccgtc ctcgctcctc tgctgaaagg tctgcgggaa cggttataac tgcgttttca
     3781 gagccttttt ccggctcgac ttcctgatga atacgaaaga ttccgtactg cagcctgatt
     3841 tggtccacct cgccaacagc taacgcggca ctgtttccca ataccgcaca accgacctca
     3901 cagcccgaaa cagcctgctc aattaagatt ttgctgtcat attgtcttgc cgattcaatt
     3961 gcgtagtcca attcgtccgc gctattgact tttttcacac cgaaggatga gcctgaacgc
     4021 gccggcttaa caaaaacagg ataggtaaac gtagctgcca ccggcctatc atctttatta
     4081 ataacccaaa aggcgggagt agctatccca gcatttttcg caacgatgta tgtcaacgat
     4141 ttgtccatac aaattgctga gctttgaata tcgcagccta caaaagggat accggacaat
     4201 tcaaacagac cttgtatgga tccatcttca cctgacttgc catgcaaagc tgaaaatgct
     4261 acatcaacat ggttgatttc atattcatgg ttctttttaa caagtaatcc gtgcattttt
     4321 ttatccggcg agagtacagc tgaatagcaa ttgtcgtttt cccattccgc gcaaggtttt
     4381 tcgcacattt tccatacacc agatttcgta attccaatgt ataacggctc gtatttttct
     4441 ttattaatgt tagcggctat ctctattgca gattttaccg atacgtcatg ctcctctgag
     4501 caacccccaa acagtattgc aacttttatt ctattcatgc tcctgtctcc tttcaaaatc
     4561 caaacagttt ttaatggttt tttcaacggt atcacgcaac gcttgctcgg tataataggc
     4621 cgtatgcggt gtgattatca cgttaggcat tctttgaagt ttaagtaaaa attgattatc
     4681 aattggtttt tgggtgcaat cagagtagaa aaactcttcc tctccttcca atacatccaa
     4741 tgcggcaccg cccagtttcc cgttttctaa tgctttaacc aactcatagg tatctacaag
     4801 tggaccgcgc ccagtattga taagaaatgc tccttgcttc attctctgta tttgttcgtg
     4861 gctgataata tagtgcgtat ccgtattgag cggcacatga agcgtaacga tatcgctatt
     4921 ttgcagcaac tcatcaaacg gtacatagtt tacctctata cttcggctgc gactataagc
     4981 caacacttta catccaaatc ctcgcagccg ctcaataacc gctttgccta tctggcccgt
     5041 tcccaccaca ccaactgtca tgtcgctgag taccttgcca cggtcgctgt ccaacctgaa
     5101 atcatgtttt tccacagagc gcacaatcga ttttacgttg cgtactgcca taagaattag
     5161 catcatagta taatcggcaa cgctatccgg cgagtacgcc acattgtcga cagtgatgcc
     5221 cattctctta gcagcagttg tatctatatg attgcagccg atgcttcggg tagaaatata
     5281 tttcacaccg gctctcttca gcgcaagaag aatagaggcg gaaatctctg atttatgtcc
     5341 cacactgata cattgattga aaggcgcgga tttggcgttg gattccgaca cgttggcgtt
     5401 aattatcgtt gccataacgc caaagcgagg cgaaagagca tggaatgcat ctgcctcatc
     5461 ctgctcacat ccataaacag taatgccgat gttattcata attaagacca accctttctg
     5521 tgaaaggcac caaataaaac ggggatgcca atggtaatct tgtcaaatct ttctctgaag
     5581 aacgaaaacg gctcgttctt acggtattaa gtctaccgtg taagaacgag ccgtttctta
     5641 atattaaatt gttaatttcc taagaaaaag taaagataac cttgagattt tcctaaaaaa
     5701 tta
//
LOCUS       DANJMA010000093         5691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000093 DANJMA010000000
VERSION     DANJMA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            58..510
                     /locus_tag="PWU31_002576"
     CDS             58..510
                     /locus_tag="PWU31_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085046.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="PWU31_002577"
     CDS             524..1213
                     /locus_tag="PWU31_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085047.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="PWU31_002578"
     CDS             1338..3689
                     /locus_tag="PWU31_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL1085048.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="PWU31_002579"
     CDS             3769..4164
                     /locus_tag="PWU31_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085049.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="PWU31_002580"
     CDS             4489..5106
                     /locus_tag="PWU31_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085050.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="PWU31_002581"
     CDS             5147..5446
                     /locus_tag="PWU31_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL1085051.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..5647
                     /locus_tag="PWU31_002582"
     CDS             5480..5647
                     /locus_tag="PWU31_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1085052.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT         1996 a          751 c         1161 g         1783 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     5641 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt t
//
LOCUS       DANJMA010000094         5690 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000094 DANJMA010000000
VERSION     DANJMA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(489..1298)
                     /locus_tag="PWU31_002583"
     CDS             complement(489..1298)
                     /locus_tag="PWU31_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1085053.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1298..2044)
                     /locus_tag="PWU31_002584"
     CDS             complement(1298..2044)
                     /locus_tag="PWU31_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1085054.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2062..2529)
                     /locus_tag="PWU31_002585"
     CDS             complement(2062..2529)
                     /locus_tag="PWU31_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1085055.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2529..2939)
                     /locus_tag="PWU31_002586"
     CDS             complement(2529..2939)
                     /locus_tag="PWU31_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1085056.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2950..3963)
                     /locus_tag="PWU31_002587"
     CDS             complement(2950..3963)
                     /locus_tag="PWU31_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1085057.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4180..4914
                     /locus_tag="PWU31_002588"
     CDS             4180..4914
                     /locus_tag="PWU31_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1085058.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4898..5608
                     /locus_tag="PWU31_002589"
     CDS             4898..5608
                     /locus_tag="PWU31_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1085059.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1835 a         1194 c          943 g         1718 t
ORIGIN      
        1 aaagcgacaa aaaccacgag caggtccatt catcctcaaa cctcggtgat cttcgcggtt
       61 taacaccatc agaagaaatg cacaaaaata ataaggagag tctgagacat aaataaattc
      121 ccagactttt agtgagtcta aatccgaata aacggtgggt cgaaagcaac tctagctgcg
      181 cgtcgatcgc agcaatctct aggtgctatg ttcggataaa tggtggtcaa aagtcagatt
      241 ttcggccatt tcgtaacagc caaccagaca aaaagcgtct gttttgtctt ctactccaat
      301 tgctcaaatc ttagcgactt ttggcttggt cttactctaa acagtttgta agcctaaact
      361 ttatttgctg tttgtttcta agatcaaagc aaaactactt tttagttggt tacttttaca
      421 agttcttttt actgttgaat aaatgtaaat cttaagaaca aatatttact tctataattc
      481 caagtttctt aaaaaattcc gccaagtgta cctaaaaagc ataaaacaaa aattcctaga
      541 ataattgtga tcgttttatg ccctttggag taaagccaga cgcataaaaa ggtaattcct
      601 aatggcagca agcccggaaa aatttgatca aaaatacttt gtaatgaagt ttcagctcca
      661 ccaaccgtga attttagtga cgtatttaaa gtaaccatcg tgcctatcat cgcgccaatt
      721 accgttaaac ctaaaatcga agcaccatga gataatttgt caatcatatg gttgtcttct
      781 gtatcatcaa taaatttagt tcctaaaaca tagccatatt ttgtcgccaa ccatttaaaa
      841 ataaaatgaa tggcagtata caaagtaaaa taaaggatca tgcctaaaat attcccttgt
      901 tgagcaaaag aaatacctat gccagccgca ataacccgaa aagttcccca gaaaaatgaa
      961 tcgccaatac ctgataaagg tcccattaat cctgttttga ttttattaat tgcgtctgta
     1021 tcaaattcag gatttttagc attttcttct tccatggtga cggacaagct cataacaaaa
     1081 ggcgcaatat gcggtgtaat gttaaatgtt tccgtatggc gctgcaacgc tgtataataa
     1141 tcatcatcgt tcggatatat tttacgtaag gaacgttcaa taccattcat ttgtccatat
     1201 gccatcattt tatcgaagga ccaagaggat tgcataaaaa aggagcgcca aaatatactt
     1261 cttaattctt ttttcccaat aacactttct tttaccatta taaatcttcc tcctcttcaa
     1321 aagttagctt ttgaacagcc agtaacgcta agcaaagtcc gacgacagcc acaccgacaa
     1381 catccatttt cagatacatt acgccgacaa atcctaagaa aagataaggc atcatcgctt
     1441 tattcgccat cattttaatt aataacgcta gcccaacaca agaaagcaca ccggccgcaa
     1501 catcaaagct gtccaataca acctgaggaa tcaacttgat catcgcattg attgtagaca
     1561 caccaaaata agtagctaca aaaactaaaa tggccgatgg aaccccaacg attaaaggtt
     1621 gagccacata atgatatttt tgtgcttttt tgaaatcatg attgactaat gcctcatcca
     1681 ttttgttgtt aaaatacgta attggcattg cataggcgaa gttacgccac atctgaagaa
     1741 agatggacaa aggtaaagtc aacgcaacgg cagttcctac tccttgaccc gattgaatcg
     1801 caattgctgt acctaaaaca ctggaagcat aaacttcagg gggcgtcgca ctccctacca
     1861 taattgagcc catgaacatg gattccaagg tggcaccaat aataattcct gactctaaat
     1921 ctcccaaaat caaaccgaca aaaggcgctg taaatagcgg tctacccatc atcgatgcac
     1981 caaatgtgta ttcatcaata gacgccaaaa aagctaccag tgcgattaat attgcttgta
     2041 tcatattctt cccctcctct tttagtcatg caaaggattg atttttttgt ccgttggtac
     2101 agcctgacat tgaactgtta tcccgttttc agtcattttt ttcaccaagt ccagttcttg
     2161 ttcattcaag taaacggaac tagtaatcat tttccgattc tcgtttttct ttaaatttcc
     2221 taaattcact tctttaatct caggtacgtg ctgagtaatt tcgtaggcat cctccaccgt
     2281 cccgacaatt aaaaatactt ggtatttctt gattttttct ccatttaaaa cagagatggc
     2341 ctctgtcata tccttcacca caaattttgt ccctgtagga acagccattt ttagtgtttt
     2401 tttgcgcaga ttattggtag cgatttcatt acttgcaacg aaaatacaat ttgcacctaa
     2461 gttatttgtc caggcaaaag caacttgccc atgaacaaaa cgttcatcta ctcgtaataa
     2521 tttaatcatt aaaaatcatc ctcttctgtt ttctctagca actcgttcac caaaactatc
     2581 ccttcattcc cgctattcat agctttttgg attagttttt tcgtgtcctg ttcacctgat
     2641 aagcaaagct ctaaaagcag cggcagattt acaccgctga ttaaataaaa attgtcttta
     2701 ccgatttgtt cggagaaagc gttagacacg cttcctccca gtaaatctac cgtcacaatt
     2761 aattcgtcat caggcgctaa gtcggaaaat agctgctcta tttcctggtt aatggagtag
     2821 tcactatcta tatacgcagt tattgctaaa agtgattttt gaggaccaat aataaattct
     2881 aaagtatcct ttaagccttt agccatcatg gaatgcgtcg caattacaat tttcctcata
     2941 gctcttacct taatattcca cagtccgata ataacgacga atatctagtg aatgatcccg
     3001 caccttttca aaatgagcac tcactcgttc taaggccgtt gccataacca ttggcgctac
     3061 atactgacgc atagcgcttg gtacacctgc taattcgaaa tccgcagtgt caaaaaccgt
     3121 cacaacatcg ctatatttat ttacaaaacg ctctacgcga tcgactagcg gacgcgtttg
     3181 atcttcacct tttaataaca caaaactcat agatttatca gtcatttcaa tagtcccatg
     3241 gaaaaattcg gctgcatgga tcgacttggt ggcaatccat tgcatttctt ccagtacgca
     3301 catagcatat tcataggcag ccccataagt attcccggct ccaacaaaca tatgaaattc
     3361 cgtattttta tgtttttctg caaattcaag tgctttctga tcattttgtt gccgaacact
     3421 tagtaatact tccggcattt tttgtaatgt ttgaataaat tcatcatatt caggaaactc
     3481 accgttattt ttcatcaggc gtgcacccaa agcaaaaaac tgtaactgaa gttcttctac
     3541 taatgtatcg ttgtcagcat cattttcaat cgcataatcc acgatttccc cgagtggtgt
     3601 agcagcattt cctacaaacg caatcgtcgt tgcaccttga ctattggcat acttaacaac
     3661 atcaacggtt tcttgagtcg taccggttaa tgaagatgtg attaacactg tatctttatt
     3721 aatctttttc ggctgtgaac aaaccacctc tgcggctatt tcatgataat aatcaagctc
     3781 tgaatgagtg cttaacagat aagctagcgg gctgattgtg gcatatgttc caccagagcc
     3841 taccaaataa agatttttaa atcccttctc actgattgca tctgccacct gattgattgt
     3901 ctcgatattt tttactgcac catttaatac tgatagaaaa cgttcttcgc taaaacctaa
     3961 catagttaaa aacctccaag tcaataattt gaaaaatata tttgcaaatg ccggcacatt
     4021 aacaaaccac ttaacctttt ttatatacca cctgttaacg tttttaatgt accacttata
     4081 gaaaacgctg tcaatagttt ttaaacactt atatttttat ttgtaccaaa atcctttttt
     4141 catgtataat aaacaaaaat agactctgga gtgtaacgta tgaatactga agaactaacg
     4201 aacaaattaa tacaagacat tgaacaagaa aaatataaat acgcgcaagc tattccttca
     4261 gaacgtgatt ttgttcaaat ttatgaaatg aatcgttcag ccgtaaaaaa agccattcaa
     4321 caattagtaa ataaagagta cctactaaaa atacatggca aaggaacttt tgttaaaaaa
     4381 aatatccatg aacatttgca aatcaaattt catggtttgt ctgaattgtt ggaacgggaa
     4441 gggattcagc ctagcagctt aattttagct tcagaaataa aaccggctgg acacattgtc
     4501 gggaaagctt tacagctgga accaactgaa gaagtatttc atttggttcg tttaagaagc
     4561 ggcgacggtg agccaatttc tattgaagac acttatattc cattaaatct catacctgat
     4621 cttcaagctt ttgatttcca aattttttca ttttattcca cattggctgc ttatcattat
     4681 caaattgaaa atatctatca gacaatttcc agctcaaaag tgagcaataa agaagcccgt
     4741 ttgttaaaaa ccagcttaaa tcgtccggta atcagccttg ctatcactgc ggttacgaca
     4801 aatgaacaag cggttgaata cactgaagta gtggttttgc ctgaattcgc cgccatctat
     4861 actaaaaatg ttcatcaagg aaaggagatg aaaatctatg cccaaattga ttaatctgtt
     4921 ggcttatgac ctgattgaca aaattattgc ttatattcat gaacatcatt taacccccgg
     4981 aacaaaactg ccttcggaac gggacctatc agaaatgttt aatgttaatc gaactacctt
     5041 aagaggcgcg ttacaaaaac tggtggacaa cggcacctta cgggcgcaaa ccaatagcgg
     5101 ctattattta actgcgccaa aattcgaaaa aaatgcccat catttcttta caccgataga
     5161 agattctctg cttagtaagc agcggctaca gctaaaggca acggctcttt caccgaaaaa
     5221 agcagcagct ctttgtccgg aactgaatga tttcaaacag cagcaaccac aagcttattt
     5281 agaatatatt gatgcagaag caattagctt gatgttatta atccctatgg aaacattaaa
     5341 gaaaagtaat ttctcggaca atcctttcca agattttaaa agtttggcat atacgcgttc
     5401 tcaagaaatt tctttaataa ctgacaatga cgttgcggcg gacttgctgg gagttgacaa
     5461 agaagaggtt ctgttatttg tacgagagga aataagagcc ggtaaggagt tggcggcgat
     5521 tgctttaggt tatactatta gtcgccgctg caacatacag gttaatgttg ctacgacctt
     5581 gcaatccggt tctgttgcaa agttttaaat ctactatcag ataaggtaga ataatagaaa
     5641 aagatagcag gaggaatgac gatgagtcat tttaaaggaa agcaatttca
//
LOCUS       DANJMA010000095         5664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000095 DANJMA010000000
VERSION     DANJMA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..193)
                     /locus_tag="PWU31_002590"
     CDS             complement(<1..193)
                     /locus_tag="PWU31_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085060.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(736..2886)
                     /locus_tag="PWU31_002591"
                     /pseudo
     CDS             complement(736..2886)
                     /locus_tag="PWU31_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3032..3607
                     /locus_tag="PWU31_002592"
     CDS             3032..3607
                     /locus_tag="PWU31_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085061.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3821..4516
                     /gene="vanR"
                     /locus_tag="PWU31_002593"
     CDS             3821..4516
                     /gene="vanR"
                     /locus_tag="PWU31_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL1085062.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4494..5648
                     /locus_tag="PWU31_002594"
     CDS             4494..5648
                     /locus_tag="PWU31_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL1085063.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1729 t
ORIGIN      
        1 gttcaaaatc taaaatcaac acatcatggg gagtgtgata ataaatattt tcccttaatg
       61 taccaacaat ggtaattcgg tcaattgacc attgcatttt taagggggtg cacatagttt
      121 gtgcatttaa ccgcctgtta gaaatacggt taagtttttc ccactcattt acgattatgc
      181 cttttttact caaaaatagc tcctcctcaa cgaaaaaaga gattcttgga aagaatttcc
      241 agagttcacg taactctaaa aatccacatc caaaaatctc tagcttgaag aaacctaaaa
      301 atattcgtat aatgaacttg agttacggaa ctaggtgaag tagtttccgt aaaggtcgag
      361 agtgcgtcaa cactgctcga ctattttttt actctttttt ctcattttct ttgattatag
      421 cactcctcta gaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc
      481 tcctcctgcc gaaatagaat ggcgctgcca gcatttattt cgttcagcta tcggctacac
      541 aatctaacac gaccgttccg ctaccagtcg ccttacaggc tccattttcg ctcctctaac
      601 gtgtttcacc gattgtgccg ataactaaac gaaataaacg ctaaaaccgc tcagaacgca
      661 aaatatgcca ttttagagct attgtagaaa atatagtttg aaatgcaatt ttcttctttc
      721 agctttttat ttcccacttg taaatcggta gatggtggct ccttttccag ttccataatg
      781 tggatttgca tctgcatgta gtgatgaaac acctagctgc attctcatac catctgacga
      841 agatgttgta ccgtcgcccc aatagaaagg caattgtaat ttatgatgaa agtttactaa
      901 tatggcttgg gctttattca tggcatcttc atacatgcgc cattgagata cattggctag
      961 ttgcttatat gtaagtccgg gtgtggcttc ggccatcttg ctcaagccaa tattcattcc
     1021 cattcctaaa agggcagcca tgataatgat tgtttcttcc ttatctggtt ttcgattatt
     1081 ggaagcatga gtgaattgct catgaaatcc tgttatatgg gccacatcca tgagtaaatc
     1141 agttaatttt attcttggta gcatctgata aaggcttgca ctaaattttt ttgcttcttc
     1201 tggaacatct ttttctaagc gtgcaagtga tagctttcct ttttcaagag aaaccccatc
     1261 taacttattg gaattggcag ctaaccactt taacctttca ttaaagctgc tggttctctc
     1321 cgttatataa tcttcgaatg ataaactaac tgataatctc gtattcccct tcgattgatt
     1381 ccatgtatct tccgaaaaca aatattcctc aaaatcccta tattgtctgc tgccaacaat
     1441 ggaaacatct cctgcccgaa catgctcccg aagttctgtt aaaacagcca tttcatagta
     1501 atgacgatta attgttgtac catcatcctc gtataaatgt cttttccatc gttttgaaat
     1561 aaaatccaca ggtgagtcat caggcacttt tcgctttcca gattcgttca ttcctcggat
     1621 aatctcaaca gcttgtaaaa gtggctcatt tgcctttgta gaatgaaatt ccaatactct
     1681 taatagcgtt ggcgtatatt ttcttagtga ataaaaccgt ttttgcagta agtctaaata
     1741 atcatagtcg gcaggacgtg caagttcctg agcctcttct actgaagaga caaaggtatt
     1801 ccattcaata accgattcta aaaccttaaa aacgtctaat ttttcctctc ttgctttaat
     1861 taatgcttgt ccgatgttcg taaagtgtat aactttctca tttagctttt taccgttttg
     1921 tttctggatt tcctcttgag ccttacgacc ttttgataac aaactaagta tttgcctatc
     1981 atgaatttca aacgctttat ccgttagctc ctgagtaagt tgtaataaat agatggttaa
     2041 tatcgaataa cgtttatttt cttgaaagtc acggaatgca tacggctcgt atcttgagcc
     2101 taagcgagac agctgcaaca ggcggttacg gtgcaaatga ctaatttgca ctgtttctaa
     2161 atccattcct cgtatgtatt cgagtcgttc tattattttt agaaaagttt cgggtgaagg
     2221 atgacccggt ggctctttta accaacccaa tatcgtttta ttggattcgg atggatgctg
     2281 cgaggtaata atcccttcaa gcttttcttt ttgctcattt gttagagatt tactaaccgt
     2341 attaaatagc ttcttttcag ccattgccct tgcttcccac accattcttt caagtgtagt
     2401 gatagcaggc agtataattt tgttttttct tagaaaatct atgcattcat gcagtagatg
     2461 aatggcatca ccattttcca aagctaattg atgaaggtac ttaaatgtca ttcgatattc
     2521 actcagggta aaagttacaa agtcgtattc acttcgaatt tctttcaaat gatcccaaag
     2581 tgtattttcc ctttgaggat aatgatcaag cgaggatgga ctaacaccaa tctgtttcga
     2641 tatatattgt atgaccgaat ctgggatgct tttgatatga gtgtatggcc aaccgggata
     2701 ccgaagaaca gctaattgaa cagcaaatcc taaacggttt tcttccctcc ttcgcttatt
     2761 aactatttct aaatcccgtt tggaaaaagt gaagtaggtc cccagtatcc attcatcttc
     2821 agggatttgc ataaaagcct gtctctgttc cggtgtaagc aattctctac ctctcgcaat
     2881 tttcattcag tatcattcca tttctgtatt ttcaatttat tagttcaatt atatatcaat
     2941 agagtgtact ctattgatac aaatgtagta gactgataaa atcatagtta agagcgtctc
     3001 ataagacttg tctcaaaaat gaggtgatat tttgcggaaa atcggttata ttcgtgtcag
     3061 ttcgactaac cagaatcctt caagacaatt tcagcagttg aacgagatcg gaatggatat
     3121 tatatatgaa gagaaagttt caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt
     3181 agacgattta caggaagatg acatcattta tgttacagac ttaactcgaa tcactcgtag
     3241 tacacaagat ctatttgaat taatcgataa catacgagat aaaaaggcaa gtttaaaatc
     3301 actaaaagat acatggcttg atttatcaga agataatcca tacagccaat tcttaattac
     3361 tgtaatggct ggtgttaacc aattagagcg agatcttatt cggatgagac aacgtgaagg
     3421 gattgaattg gctaagaaag aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa
     3481 tcacgcagga atgaattatg cggtaaagct atataaagaa ggaaatatga ctgtaaatca
     3541 aatttgtgaa attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa
     3601 taattagcca ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat
     3661 aaaatattaa cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat
     3721 cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga
     3781 taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat
     3841 tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta
     3901 tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat
     3961 tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa
     4021 aataagggac aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga
     4081 taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact
     4141 ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa
     4201 ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga
     4261 gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat
     4321 cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg
     4381 cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga
     4441 aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta
     4501 taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata
     4561 tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag
     4621 ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg
     4681 acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg
     4741 cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact
     4801 ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc
     4861 tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa
     4921 agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg
     4981 cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg
     5041 ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt
     5101 atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga
     5161 taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat
     5221 tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga
     5281 ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg
     5341 ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg
     5401 tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg
     5461 aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat
     5521 tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg
     5581 ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga
     5641 ggtcctaaga gatgtatata attt
//
LOCUS       DANJMA010000096         5522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000096 DANJMA010000000
VERSION     DANJMA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..219)
                     /locus_tag="PWU31_002595"
     CDS             complement(<1..219)
                     /locus_tag="PWU31_002595"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085064.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="PWU31_002596"
     CDS             complement(330..584)
                     /locus_tag="PWU31_002596"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085065.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="PWU31_002597"
     CDS             complement(777..1052)
                     /locus_tag="PWU31_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL1085066.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="PWU31_002598"
     CDS             complement(1024..1977)
                     /locus_tag="PWU31_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1085067.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="PWU31_002599"
     CDS             2589..4082
                     /locus_tag="PWU31_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL1085068.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="PWU31_002600"
     CDS             4217..4513
                     /locus_tag="PWU31_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL1085069.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..5496)
                     /locus_tag="PWU31_002601"
     CDS             complement(4537..5496)
                     /locus_tag="PWU31_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1085070.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1776 a          939 c          962 g         1845 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc
     4681 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     4741 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg
     4801 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg
     4861 tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg
     4921 tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg
     4981 tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata
     5041 atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg
     5101 tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt
     5161 gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat
     5221 cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg
     5281 taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc
     5341 taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg
     5401 attcaagaaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat
     5461 tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt
     5521 tg
//
LOCUS       DANJMA010000097         5456 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000097 DANJMA010000000
VERSION     DANJMA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..556
                     /locus_tag="PWU31_002602"
     CDS             <1..556
                     /locus_tag="PWU31_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HDL1085071.1"
                     /translation="NLIRGEFHFLSEKDMNIIPSFKDSGYIPDTKAMLIYDETGQLEN
                     YLSEISGARPNRLTEENANFLLCNFSNLWLMGINVLKRGEYARSLELLSQLQKNTLQL
                     IRMAEKNADNWLNMSKNLEKEISLENYKKFAKTTARLDKVELFEAYKNSLLLVMDLQS
                     HLIEQYNLKVTHDILERLLNYISE"
     gene            1244..2065
                     /locus_tag="PWU31_002603"
                     /pseudo
     CDS             1244..2065
                     /locus_tag="PWU31_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2208..2432
                     /locus_tag="PWU31_002604"
     CDS             2208..2432
                     /locus_tag="PWU31_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085072.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            2447..3316
                     /locus_tag="PWU31_002605"
     CDS             2447..3316
                     /locus_tag="PWU31_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL1085073.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3297..4031
                     /locus_tag="PWU31_002606"
     CDS             3297..4031
                     /locus_tag="PWU31_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1085074.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4064..4972
                     /locus_tag="PWU31_002607"
     CDS             4064..4972
                     /locus_tag="PWU31_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL1085075.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4969..5217
                     /locus_tag="PWU31_002608"
     CDS             4969..5217
                     /locus_tag="PWU31_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HDL1085076.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(5220..>5456)
                     /locus_tag="PWU31_002609"
     CDS             complement(5220..>5456)
                     /locus_tag="PWU31_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL1085077.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1865 a          821 c         1159 g         1611 t
ORIGIN      
        1 taatcttata cgtggggaat ttcatttcct ttctgaaaaa gatatgaaca taatcccctc
       61 gtttaaagat tcaggttata ttcctgatac gaaggctatg cttatttacg atgaaacagg
      121 gcaattagaa aattatttat cagagataag tggtgcaaga ccaaatagac ttactgaaga
      181 aaatgctaat tttttgttgt gtaatttctc taatctatgg ttgatgggaa tcaacgttct
      241 aaaaagagga gaatatgctc gttcattaga actcttatca caacttcaaa aaaatacact
      301 acaacttata cgtatggcag aaaaaaatgc tgataattgg ctaaacatga gtaaaaacct
      361 tgaaaaagaa attagccttg aaaattataa aaaatttgca aagaccactg ctcgattaga
      421 taaggtagaa ttatttgaag cctataaaaa ttctttgcta ttagttatgg atttgcaaag
      481 tcaccttatt gaacaataca acttaaaagt tacacatgac attttagaaa gattgttgaa
      541 ttacattagt gaataggaga gtaacgttat tcacagtatt gggtactgaa aattaacagc
      601 atacctcagt ttttcaaaaa acagcgaagc ctatggatat gttcccgtct accgatagtg
      661 caaaaagtgt cgtggatatg tttccgagaa cggacataca caaatgacat tcattctgtc
      721 ctctcaatcc atgtggaagc agtatgtctg ttaacaagaa aataattatt gttcgtcctg
      781 acgagttgtt agactcgaaa aggataaaca tcatttttag aaccggtagc cgatttttgg
      841 ctagcggttt tttgggctct ttggcaaata gtggtgattg aatctcgcgc tatcctatgg
      901 aaaaccgcct tgtgtgaggg gctgcgagac cgcttcgatc ctagaattgc tgaagcacga
      961 atctagcgtc actcacattt catcttttga cgtagctgag acaaggtgtt tttcttcgat
     1021 ccgctagtgg ttaatctctt tttggtgcaa atatggttta gaatttcttt gcatggctta
     1081 agggagtgga gaatcggata ttagtaggat ttacatccac agataaaaat aggcttgatt
     1141 ttaacagcga aactaaaata ccctacttaa atgaagtgat agattagatg ttgggttgtt
     1201 aggagatgct taaaaatcct atgcaatcaa tagtttttag tgtttgttga cttgatccct
     1261 aaatcgcctg agtactaaac gttgagtgtg tctgcttact gtcactagcc agtaagtgag
     1321 actgtagtta atctctcatt ttccccagta ttatgttgaa aatagccatg aagcgattat
     1381 ttctaaagag ttatttttgc aggctcaaga agaaatttat cggagaagta atatctacac
     1441 aggagaaggc aagaacaaaa gaatttatag tagcaagtac gctttaagtg ccatcacctt
     1501 ctgtggatat tgtggcgata tttataggag aacctattgg aatattcatg gcagaaaaga
     1561 atttgtctgg cgatgcgtga ctagaatcga gcagggtccc gaggtatgta agaaccgaac
     1621 cgtaaaagaa gatgaactct atggtgcggt aatgaccgca attaataagc tacttgctgg
     1681 tggcaacaat atgataaaga cactggaaga aaatattcat gctgtgattg gagaaaccac
     1741 agaataccaa atttcagaga ttaataccct actagatgaa aaacaaaaag aactaatcaa
     1801 gctggccaat aaaggtcaag attatgaata cctggtagat gagatcgatg agatgcgaga
     1861 taagcgtcag actcttttag tagaagatgc ctccctcagt ggcgagaacg agcgcatcaa
     1921 tgagttgatt gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct
     1981 tgtaaggaag ataatccaga acgttaaggt ctatgaagac cacttcgtca tagccttcaa
     2041 accaggtatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctgaagtag
     2101 agttgtgggt tttcttttaa ttgctgatta taataatatt ttaatgacta ttgcatttat
     2161 caaccaaatg gtttatgtta ttcttagcat aaacttggag gtgctgtatg actacggcag
     2221 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
     2281 ttggccagac tccacagaat tttaataaaa aattacaacg agaaacagta accttggatg
     2341 agttgaaggc cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag
     2401 atggagatga aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt
     2461 tattcaaata gtaacagaga aattatcatc tttgcctttt atagaaggca ttgtattagg
     2521 gggctcacgt gcaagaagca cccatacaga aaattctgat atagatatcg gaatctatta
     2581 caattcagac tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga
     2641 gaatagaaac aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg
     2701 atggttagtt ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga
     2761 acaaataatc aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc
     2821 ccatggttat ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc
     2881 taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa
     2941 gaaaagcttg atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc
     3001 aaatgcgggg gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg
     3061 cttaaatcaa gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat
     3121 aaaactgctt gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat
     3181 ttttgaagta ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa
     3241 agaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga
     3301 aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc
     3361 agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta
     3421 agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg
     3481 aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa
     3541 aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt
     3601 tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact
     3661 atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta
     3721 cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag
     3781 gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt
     3841 ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc
     3901 tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg
     3961 atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa
     4021 agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga
     4081 aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct
     4141 tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac
     4201 atattttgta agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg
     4261 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
     4321 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
     4381 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     4441 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     4501 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     4561 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     4621 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     4681 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     4741 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     4801 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     4861 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     4921 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     4981 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     5041 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
     5101 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     5161 gaatatattg acaatactga taagataata tatcttttaa cccgtggtgc tagttaattt
     5221 taatatccta aagaatatct caaagttcct ccaaaacgtc gttgtgctcg acttaataga
     5281 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     5341 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgtttttctt
     5401 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtgg
//
LOCUS       DANJMA010000098         5433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000098 DANJMA010000000
VERSION     DANJMA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            155..1150
                     /locus_tag="PWU31_002610"
     CDS             155..1150
                     /locus_tag="PWU31_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1085078.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            1154..2296
                     /locus_tag="PWU31_002611"
     CDS             1154..2296
                     /locus_tag="PWU31_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDL1085079.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            2293..3720
                     /locus_tag="PWU31_002612"
     CDS             2293..3720
                     /locus_tag="PWU31_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL1085080.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(3787..3951)
                     /locus_tag="PWU31_002613"
                     /pseudo
     CDS             complement(3787..3951)
                     /locus_tag="PWU31_002613"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4361..5101)
                     /gene="istB"
                     /locus_tag="PWU31_002614"
     CDS             complement(4361..5101)
                     /gene="istB"
                     /locus_tag="PWU31_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL1085081.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            complement(5091..>5433)
                     /locus_tag="PWU31_002615"
     CDS             complement(5091..>5433)
                     /locus_tag="PWU31_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL1085082.1"
                     /translation="NLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYIGKEVEIELS
                     DKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGIRAYTRESLK
                     QYDLVEVADEE"
BASE COUNT         1819 a          790 c          955 g         1869 t
ORIGIN      
        1 aaacttattt tattttatat aaaaaagttc taaaaaaata aagttttttt cgatatgtta
       61 ttttttatgt ttattaactt cggaagacta aaattgttac tggtaatgat acatttgtaa
      121 aaataaaaaa gatgattttt aggaagtgaa gagagtgcat aaaattagtg ttatagtacc
      181 cgcatataac gtagaaaact atttagaaaa ctgcttagaa tctatttcca atcagacaca
      241 ttcaaatatt gaaataatag tagtcgatga tggttccccg gatcgaagtg gggaaattgc
      301 agaagaatat aaaaagaaag atatgcgatt aaaagtgata catcaagtga atggtggttt
      361 aagtgcggct aggaattctg gtcttgattc tgcaacagga gattttgtgt gctttattga
      421 cagcgatgat tggatagaac cagactatct tgagttacta tattttggta cgcaaactta
      481 tggagcagat atctcggtgc taaaaataaa aaagatttca agtttagaac aactaaaaga
      541 acatacaatt gctgagagaa aatgggaaat atttgataga gaacaagcaa tgaaaaactt
      601 atttataaat aataaaattg gctattctgc aaataataaa ttattcaata gaaatttatt
      661 tgataatata agattcccac tgggtaaatt aatggaagat aaggctacta cttatttgtt
      721 gatcgatcat tccgaaaaag ttgctgtcaa ttcatctgct aaatatcact attaccaaag
      781 ccctaaaagt atactgagag gaagcttcaa tcctagaatt tttgattctt ttgaagtcca
      841 tgaagatata ctagtattca tagataagtt ttatccggaa ttatcaatta atgttagaac
      901 tcgctatgtc tatgaagcaa taaggatgat gatgacaatg attaaaaata actataccaa
      961 taaagaagat tatttacgtt ctataaatat tttacaacgc tttgaaaaag aagtgaaaat
     1021 tgatcccgac ttttcaccaa aattaaaaaa attagtaaag tttttattaa taaataaaaa
     1081 cttgccttac tatatgtcaa gtagtaagat agcaagtaaa tttttgatta agatagaatc
     1141 tgtgaggtaa gagatgaaga atataaaaaa atttttttca aggaatattt tcccggtttt
     1201 tattaaaata ttacctattc aaaaaaattt aattgtattt tcaagttttg gcgggcgtaa
     1261 ttattctgga aatccaagag ttatttcaga gaaaattcat gaaaaaaaat tgaattacag
     1321 aattatttgg ttaatcaaaa aagaagcgat tataactgat ttaccagatt atattaaaag
     1381 aattgattcc gattcttttt tagctgcctt ttatttgtat acagctaagt attggataga
     1441 cgatagtcgg aaaacgatta agtatgcaaa aaggaaaaaa caattttatt ttcaaacatg
     1501 gcacggtacg cctttaaaaa aaattgaatt tgatgcaaaa gataagttac ctaaacgtta
     1561 cctaagctat gcgaaaaaag atagtgagtc aattacatat ttattatctg gtaatcgcta
     1621 tagttctgag aagtatgttt cagcattcaa tatcgttcct tctaaaatat tagaagtagg
     1681 aactcctaga aacgatgaat tagcctctag taaagagcaa gtgtttgact tgaaaaagaa
     1741 acaaatcaat gtcttatttg ctccaacttt tcgaaataat attgaggaca acggaattac
     1801 gcaactagaa tggttaggtg tggataactt acgtgaattt tttaaaaagc aacaacaaga
     1861 attaaattta atgactaaat ttcatcctaa tgttcatagt aaattagcaa ctgattctaa
     1921 aagtatagaa tatatgaaaa aacatcaaat tactttaatt aatgatggga ttcctcttga
     1981 aatgcttttt gaagagatag atgttttaat cacagattat tcatctattt ttttcgattt
     2041 tgcgctaaca aaaaaaccaa ttattctttt taactatgat gaaaaagaat atagcttaga
     2101 aagaggtttt tatatacagt taagtgagtt accaattata agcgtacaag atagttatgg
     2161 ccttgttgat gtttttaaaa accaacaaga aaagcttttg aaaagttctc aagagttact
     2221 tgattatatt ggaaattatg aaacaggggc aagtacagaa aaggttcttg agttgttaga
     2281 gagggatata aagtgaaaaa aacagtaaaa gatatatttt ataatgcgat atatcaagtc
     2341 tttttgattg ttttgccctt acttactata cctatactta gtcgtagaat aggttcaact
     2401 ggtttaggga tttatgggta tgttttctca atatcacaat ttttaatgac tgtcattgct
     2461 gtgggaatga atccttttcg tattcgaaat atcgctaaat caaggaaaga taaaaaagca
     2521 ctatctttac aattctggaa tatttacttt cttcaatttt taataggctt gagtgtttcc
     2581 tttctctata tagcctatat ttattttttt gatgtaaagt ataaaaattt atacttaatt
     2641 caattgatat ttattgcagg gctaacatta gatatttctt ggttttttca aggaattgaa
     2701 gaattttcga aagtggttat tagaaataca gttatcaaat tatcatcggt attgttaata
     2761 atcctttttg tttcaactaa agatgatttg tggatttatg tgttcattac ttcaattact
     2821 aactttttag gaagtttagt tttctggttg agtatccacg gaaaagtagg taaaccactc
     2881 tttaactcta atgtttttaa acagttgtgg aaaccagctt ttattatttt gactcctcag
     2941 ttgtttatgc aagtgtacac aactttagac aaaacaatag ttgggtattt tgtggatcca
     3001 acagcattat cttattatga tcagtctcaa aaaattgctc gtattatatt ggcagtctta
     3061 acatcaatta ctattgttat gttgcctaaa atatctaaag cacaggcaga gggaaaatca
     3121 gaaaatattt tagtttacac aaaaaaatct tttgattaca cgattattat tgcattaatt
     3181 ctttttagtg tggtgtttat caatacaaaa gaatttgttc catggttttt tggggagaat
     3241 ttcaaaccga tgacaataaa catgcttatt tcttgtctaa ttatagttgt tagtccaatt
     3301 ggtggagttt ttgctaacca atttgcaatt gctatagaaa aagataagga gtatgcttat
     3361 ccaatgatca ttggtgcgat tgtaagtatt attggaaatg tagcttttgt gcctacttat
     3421 ggcgctgtag cggctacaat cgtactcgtg gtagtagaat tattagtctt tttctttaga
     3481 gtattttttg tacgaaaaga aataaaaata tcctatttct ttgggaagaa cgttgtctta
     3541 ttttttatag tcaatcttac tttgactact tcggcctatt actttcttcc acaattattc
     3601 tcttcttcat ttttagatat ggcaattaaa tcaataatga ttttcattat ttatcttccg
     3661 tttgtttatt ttctttttcc agatatacga aatgaagtaa gaaagattat tcgaaaataa
     3721 aataatgttg taaaaaagta gttctttgtc aaatcgggct gataaggtca tttatgatca
     3781 acaaagttag taagatttac tagcttttaa tgcatagctt attaacccgt tgattagaaa
     3841 aatccacaag cgcatattgg taaaactgca aaatccataa gcaaatcgtt tcaatgtttt
     3901 gatatgtgtg cgaagattct ctaattttgt attaaaatac tttttcgaaa atgctagtta
     3961 aaagcctttt tcagatatga aaaaggcttt ttaagatagt gaagtttcga gggaacttct
     4021 ttggtcattt ctcgaagtag ctagctgttt cttgtgataa ttgttaagca gcttttgata
     4081 atactgctaa gccattttaa gttcataaga gattgagggt agacaatcaa ctacttcact
     4141 ttctgttaaa tagcgccatt gaaaattctg ccatttcttg aatgttgtat aatccaactc
     4201 gtcatctgat tatagaagta atttatacaa aggtttcaat tgtcgatagc acttagcttg
     4261 tgaagtgtct ttttgatgtc aagggcagct taaaattgta ggttttgggc aggataaaaa
     4321 tgtaggtttt cggcaatagt aattgtcgac aatcgacact ttagatcttg tctctcatac
     4381 gataagactt gcctgtgatc ttgaacacct tgacgtgatg tactaaacgg tcaaggatgg
     4441 cagctgcgat ctcagaactt tggaacaact ccccccaacg agataagaca atgttcgtcg
     4501 taatgatcgt agagcggtgt tcatagcgca tggcaagaag ctgaaagaat aaatcagctt
     4561 cctgtttttg aatcggaaga tagccaatct catcaataat caagacgtca tagcgagcgt
     4621 atcgtttgag aacacgctct agggtacctt tttcataggc tgttcgcaga cgcaaaagca
     4681 gctcatgaca ggtgatgaat agtgtccgat agccttgacg acaggcttcg attcccaatg
     4741 caatcgtcag atgtgtcttg cctacaccag gactgccgat aaaaaccaag ttctcatgtt
     4801 tttccacaaa gcccatgtgc cttaagtcta gcacttcgcg cttattgata cttggctgga
     4861 aggcaaaatc aaactcttcc aggctggttc tcttggaaaa gcgggcgcgt gccaccacac
     4921 gattcatttt ttgcttattt tgatgatcaa cttctctgcg tgtcagctcg agaagagctt
     4981 ccgttaaaga aagctcctct cgattggcca cttcgatgta gttaggaaca tattcccgca
     5041 tatgattcaa accaagagtc tctaagttgg tcatcagttc ttggtaatgg ctattcttca
     5101 tcggcaacct ccaccagatc atattgcttg agactttcac gagtatacgc tctgattcca
     5161 tcgtcttctt tgtgactcat gacatcggat ttcaagattt ggaaatagtc ttcctcgtgg
     5221 tagttcagct gttttgtcgt taatggatgc gaccgaatca gttctccgtt ataatagatt
     5281 tgtacttggt cttctttatc ggagagttcg atttcgactt ctttaccgat atagcgaggc
     5341 gccacagagt acctgcattt acggaaaatg accatttctt ccttggtgac tttacggcga
     5401 ataaagtctt caaaataggg attcaaaagg ttt
//
LOCUS       DANJMA010000099         5340 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000099 DANJMA010000000
VERSION     DANJMA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(62..280)
                     /locus_tag="PWU31_002616"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="PWU31_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="PWU31_002617"
     CDS             complement(546..740)
                     /locus_tag="PWU31_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085083.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="PWU31_002618"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="PWU31_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="PWU31_002619"
     CDS             2092..3132
                     /locus_tag="PWU31_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1085084.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="PWU31_002620"
     CDS             3956..4288
                     /locus_tag="PWU31_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL1085085.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4904..5245
                     /locus_tag="PWU31_002621"
     CDS             4904..5245
                     /locus_tag="PWU31_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL1085086.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          727 c          900 g         1771 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt
     4801 taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattaatat
     4861 aaagcataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc
     4921 gaatatacga ttgggcacac atactattaa ggtttctttc caacgttggg attacaaagg
     4981 atttgtgacg tttagaaggg gcggaaattg taaaggatta gatgttttag cattagacga
     5041 ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga
     5101 agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt
     5161 atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga
     5221 atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta
     5281 gaaggagtca cgacgattaa taaaaaggta gtattacctt aggcattgag taaggaattt
//
LOCUS       DANJMA010000100         5239 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000100 DANJMA010000000
VERSION     DANJMA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(3..1151)
                     /gene="nagA"
                     /locus_tag="PWU31_002622"
     CDS             complement(3..1151)
                     /gene="nagA"
                     /locus_tag="PWU31_002622"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL1085087.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1227..2063)
                     /locus_tag="PWU31_002623"
     CDS             complement(1227..2063)
                     /locus_tag="PWU31_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085088.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2247..3464)
                     /locus_tag="PWU31_002624"
     CDS             complement(2247..3464)
                     /locus_tag="PWU31_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1085089.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3613..3792)
                     /locus_tag="PWU31_002625"
     CDS             complement(3613..3792)
                     /locus_tag="PWU31_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085090.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3847..3930
                     /locus_tag="PWU31_002626"
     tRNA            3847..3930
                     /locus_tag="PWU31_002626"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3881..3883,aa:Leu,seq:gag)
     gene            complement(4170..5168)
                     /locus_tag="PWU31_002627"
     CDS             complement(4170..5168)
                     /locus_tag="PWU31_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085091.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1621 a         1072 c          831 g         1715 t
ORIGIN      
        1 tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg
       61 ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt
      121 ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat
      181 ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg
      241 ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa
      301 tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt
      361 cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc
      421 aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga
      481 ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct
      541 ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca
      601 gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg
      661 attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa
      721 taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt
      781 cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct
      841 tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat
      901 aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc
      961 atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca
     1021 atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta
     1081 atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata
     1141 aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag
     1201 aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt
     1261 gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt
     1321 ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt
     1381 ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca
     1441 taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc
     1501 agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat
     1561 atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt
     1621 tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc
     1681 aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac
     1741 agattttcag aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt
     1801 tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag
     1861 agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat
     1921 acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca
     1981 tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca
     2041 aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat
     2101 tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac
     2161 tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct
     2221 cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc
     2281 attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga
     2341 tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa
     2401 aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg
     2461 aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca
     2521 ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca
     2581 ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa
     2641 cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca
     2701 cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc
     2761 agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc
     2821 atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact
     2881 tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta
     2941 atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga
     3001 taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata
     3061 gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc
     3121 aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg
     3181 atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa
     3241 aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga
     3301 atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa
     3361 atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg
     3421 taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct
     3481 catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc
     3541 catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca
     3601 agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc
     3661 aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat
     3721 caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat
     3781 aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat
     3841 tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag
     3901 ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac
     3961 gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca
     4021 aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct
     4081 actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc
     4141 taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta
     4201 caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt
     4261 ttcattagct ctttaactgc cattagtact aagatggcga taacaacata aagtatatag
     4321 tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa
     4381 aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa
     4441 taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta
     4501 cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta
     4561 ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta
     4621 ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa
     4681 tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag
     4741 atattcatat ctaaaaaaaa cggctttcca aatgatgaaa tacctaaatc taacttatta
     4801 agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc agcacgtaat
     4861 gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat tgaaaatact
     4921 gataacttat aattatatcg tccattgaca atcaaatata aactaactat acttgttaga
     4981 acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa aggaatactt
     5041 acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa cggataaaaa
     5101 gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct ccaaccggtt
     5161 tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa taaattttta
     5221 acatttattt tctcaaaaa
//
LOCUS       DANJMA010000101         5177 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000101 DANJMA010000000
VERSION     DANJMA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(137..1099)
                     /locus_tag="PWU31_002628"
     CDS             complement(137..1099)
                     /locus_tag="PWU31_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1085092.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="PWU31_002629"
     CDS             complement(1101..2540)
                     /locus_tag="PWU31_002629"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL1085093.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="PWU31_002630"
     CDS             2725..4671
                     /locus_tag="PWU31_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL1085094.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4935..>5177
                     /locus_tag="PWU31_002631"
     CDS             4935..>5177
                     /locus_tag="PWU31_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1085095.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1669 a          878 c          853 g         1777 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4861 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4921 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4981 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5041 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5101 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5161 ttacctcgac cccgaaa
//
LOCUS       DANJMA010000102         5136 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000102 DANJMA010000000
VERSION     DANJMA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(143..1087)
                     /locus_tag="PWU31_002632"
     CDS             complement(143..1087)
                     /locus_tag="PWU31_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL1085096.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1221..2612)
                     /locus_tag="PWU31_002633"
     CDS             complement(1221..2612)
                     /locus_tag="PWU31_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL1085097.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2738..3148
                     /gene="tagD"
                     /locus_tag="PWU31_002634"
     CDS             2738..3148
                     /gene="tagD"
                     /locus_tag="PWU31_002634"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDL1085098.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3324..3737
                     /locus_tag="PWU31_002635"
     CDS             3324..3737
                     /locus_tag="PWU31_002635"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085099.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3894..4151
                     /locus_tag="PWU31_002636"
     CDS             3894..4151
                     /locus_tag="PWU31_002636"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085100.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4238..>5136
                     /gene="istA"
                     /locus_tag="PWU31_002637"
     CDS             4238..>5136
                     /gene="istA"
                     /locus_tag="PWU31_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL1085101.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQK"
BASE COUNT         1789 a          931 c          810 g         1606 t
ORIGIN      
        1 aaagttctct ttttcatgta aaatatttaa ttatatattt cctaaataaa aagtaaaaca
       61 agaattaaat agttggttat acaactttaa ttcttgtttt actttttaga attatttatc
      121 caaagatgca atcatttttt catcaaattt ctgtctttaa ataccgttcc attccgctca
      181 atatatcaaa tttaggctga tagcctaaag aacgaatttt tgaaatatcc gctaaggaat
      241 cacgaatatc ccctgaacgc tccgcttcat atttaagcgc tagctctgtt cctaagattt
      301 gatcaattga atggatcaat tcaagtagcg tagtgctttt tcctgtaccg acattgaatt
      361 gctgtcctaa agctttttct tccttggcaa caagtaaaag tgcttgtata acatcttcta
      421 tataaacaaa gtctctagac tgactcccat cgccaaataa agtaaattcc gctttttctc
      481 ctgccaattg ttttttgtaa cgatccacta gaatcgaaat gactccgcta tatggagagt
      541 ttggattttg atttggacca taaacgttaa aaaatcggac agcactagtc ggaacatcat
      601 ataaatgaca gtaatccaaa acatattgtt ctgctgcaaa tttatcgata gcatatggtg
      661 ttaatggacg gatgactgat tcttcttttt ttggcagtgt tggttcatca ccataaactg
      721 cagctgatga actaaatact aatcgtttta aattagattg atatttccgt gctaactcaa
      781 gcaataaaag cgcactgtca aaatttacac gatgtgtttc tacaggccgt tctacagaat
      841 ctgccacact agctacagca gctagatgaa aaatataggc aaactgatat tctttcatga
      901 ttctttccat caattgagaa tctgatacat ccccctcaat gaaagtcact tgtttatcca
      961 tatccaagtt ttcttttttt cccattgata aatcgtcaac tactacgatt ttgttttctt
     1021 ttcccaaata attcgctagc gttgaaccga taaaaccagc tccgccagta atcaatatag
     1081 tgtccatcaa attcactcca attgcacatt ttcgtatcaa ataatattag tttaccattt
     1141 ttatattgta aaaactataa aatttgttga agttgggata actagttttc ttttctactt
     1201 tcatatacct aaaaatagtt tcaattagtt actccttctt ctttagatat agttttttgg
     1261 atagaaccca gatatcccca aaaaataaat gaacctatcg tactaaccaa aatgagttct
     1321 gtgataaata atgccgaaac tgctattgta agaatcatta aaatagcaaa aaagtgctta
     1381 tcttttacta gatttggttg tgtgaaacaa taacgcaagg ctttaaagca ttggataaat
     1441 aagaaggtta acaatgtcaa tagacctaat cctcctgtag cagccatcac taagaaaata
     1501 aaattatgag cagttaaagt atcttttccc attaatgtat atggtagttt atcatgtgca
     1561 tattcaatat agtagcctgg agaacctccg gatatcggct tactcttgaa tatttcaatt
     1621 gcacttttcc aaagttcaaa ccttaaatta gaaacatccc cgctatcagt gtcagggcgt
     1681 tttaaatcaa ctttattgct gctagttgtt cctgatgaat cagatgtatc tttttttact
     1741 tctgtaatat gcggaacatg tgcccatgca tgagcagtta tatctgttac ataatacatc
     1801 gcaattatac ttagcaatcc tactactaat gcagttagcc atctttttaa agtattagtt
     1861 ttaagaaaac gactactata acttgtaaag aaactaaaaa caaaagttgt tattaataaa
     1921 caaactaatg tggtgcgtga acccgataaa gcaataaacg agaattgaat aaaaatatta
     1981 attacaaaaa atattttaaa tagaagattt aaatgcttgt aagtgaccaa ataatatata
     2041 gcaataatga tgacgattaa tgatataacc gcaccaaaat ttggatctgt atatacacca
     2101 aataaacggt tgcctaacca ccctaaacga actgggtaat acttatattt tattggaatt
     2161 tttatgccat attgaatgac aaaccagtat aatgatacta aattagctaa aaaccatacc
     2221 aatgaaagta tccaggtaaa tactttgaaa aaaaagttac tactatagtc tttgccaaac
     2281 tgataaatca taaagaaaaa gattaattgc cacatgatta acttaatatt ttcgctatat
     2341 ccatatttta aattcattaa actgcttact agcatcgcgc caataaaagc aattaatagc
     2401 caatcatatt tttttagatt tttttctcta aatgcaataa ttgcatctat aatgatcaag
     2461 acaactcccg ctattccagc aggccaaaaa agtaaagtat catatctagt aggtaatagt
     2521 aaatacgggc ctattgctcg taacattaaa ataaaaccaa aataaataaa ataccaacct
     2581 ttcacatatt ttaaaaatct ttcattcaac atttttcctc catccaccta tttctcataa
     2641 atatttctta tcctttttag gataaactgg tcctttcaat tctaacaaaa aaatgctaaa
     2701 ctaacaatgt atattaatga atttcgagag gtgaaagatg aaaagagtaa ttacttacgg
     2761 tacctttgat ttgcttcatt acgggcatat caatttactg aaacgagcaa aacaatacgg
     2821 agactatctt attgttgccc tttctacaga tgaattcaat tggaatgcga aacaaaaaaa
     2881 atgttatttt agttatgaaa aaagaaaaca gttacttgaa gctattcgct acgttgattt
     2941 agttattcct gaagaaaatt gggaacaaaa agtaaaagat ataaaagaat accatgcaga
     3001 ttatttcgtg atgggcgatg attgggctgg acattttgat gaacttgaac aactaacaga
     3061 tgcacaagtg atttatttac caagaacacc tgagatctct acaactcaaa taaagaacga
     3121 acttaattct ttaaatagtg ataaataata ttttttaaac aataaaagaa caatatatct
     3181 ggataagacg taaaaaaatg gctctataat ttctaggact gatagaattt tctgtcggtc
     3241 ctagattttt tgtaaaaaaa aacagacata aaaaaattcc gatagaatag agtcgtctaa
     3301 aacatcaatc tgaaaggaag tttttgatat ccatttctca ttctatcaaa gagctcttca
     3361 ctattaaaga taaaaatatt gaaattaatt cttttcaaga aactttttat aaggacaaga
     3421 aagccatcgt aatcgaaggt ccattagttc gcacttttag acattgtcta ttttgtaaag
     3481 aatctgcaaa gcagatcgag aaaaacggca agaagatttc aatgattatc ttaaatcgca
     3541 gtgggaataa acaaacctat ttgcgtctta gaaaacaaca ttaccactgc caatcatgcg
     3601 ggaaatattt tactgctcat acgtatcttg tcgctccttc atgttttatt tcgaaacaag
     3661 ttcattacac gatattagaa gagttgaccg agcgtcaagc attaaagaca attgcgaaac
     3721 gttgtgattt tcagtaacga cagttcaacg gacattatct gctctatcgg caacgctgcg
     3781 acagaagcta gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat
     3841 tttccatggg aagttctcct tttcttgtat gaattgagag acagggaagc tctttgacat
     3901 tttgtccagc cggagaaaaa aaagatttga ttgcttattt catgcgtttt acaagaaaag
     3961 ctcatatggg ggttcgatac attgtcacag atatgaatgc cccttatttt tcattagtca
     4021 aagaaccctt cccaaatgca caaatcatca ttgattgctt tcacattggg caacacttga
     4081 acaagttttt cgatcgtgtt cgaaaacgtg tgatgaagca gttgaatcaa aaatgtcaac
     4141 ggcaccttta aaatgtagga aaacggcaat ttaaaattgt tggtttacct acatttttgt
     4201 atgctcttct tatcccaaat gaaatggagg ctcagggatg agaaaagaca tacgcgaagg
     4261 agtgatgatc tacgtgatca atgaaatcaa acccaattat gctgcactag caaaacaata
     4321 cgactgcgac taccgaacag ttaagcatgc ttacgaagaa gcccaagtta aagaatcaaa
     4381 acctccagaa cgtaagaagc gtccaagtaa gttagatccc tacagagaaa tcatccaaga
     4441 caaaatcaaa gaccaatgca gtgcttacag cattttcaag tttattgagc acaaaggatt
     4501 tggaggaagc tacagtttag taaagaccta ttgtcgccag attaagcagg cgaaagtaca
     4561 caaggcaacc gtccgagtcg aacatagccc tgggctttct gctcaggttg actggaagga
     4621 agatatgcga ctaatcagta atcagggcga aatttttgag ttcaatatct ttttgtacgt
     4681 cttgccatat tccaagaaaa aatatgtcac actaactttc gaccgaaaac aagacacact
     4741 ctttgagtgc ttagatgatg cgttttatca aaccggtggc gttcccaaag aaatctggtt
     4801 cgataatatg aagacagtcg ttgaccattc tcgtagtcag ttttccaaac cgcatttcaa
     4861 caaacgtttt tatgctttca gtaaggatgc cggcttcaca ccaattgcct gtcggccatt
     4921 tcgtccgcaa acaaagggct gtgtcgaagc tttagccaga actgttgacc gcctacgggt
     4981 ctataatcat gaattttatg atgccgttga gctgtgtcat ttagtgaata acctttgtgt
     5041 tgagttaaat aacgaggttt ctcaatcaac tgatcaaaca ccagatgaac gttggcagac
     5101 gaacgaaaaa gagcacctgc atccatacca aaaaaa
//
LOCUS       DANJMA010000103         4466 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000103 DANJMA010000000
VERSION     DANJMA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            575..1009
                     /locus_tag="PWU31_002638"
     CDS             575..1009
                     /locus_tag="PWU31_002638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085102.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2299..3222
                     /locus_tag="PWU31_002639"
     CDS             2299..3222
                     /locus_tag="PWU31_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1085103.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3238..3777
                     /locus_tag="PWU31_002640"
     CDS             3238..3777
                     /locus_tag="PWU31_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1085104.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1672 a          670 c          754 g         1370 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaaataa tgagagttta ttcttgaaaa agcaacgcgc
      241 ttataaagcg ctaccaaaat tttatcatat tttataaaaa ataacaatcc agtaaaaaat
      301 gactggtaaa aattcacaaa gaaacggaaa gaagcaacta cagaaaatca agccttaaaa
      361 agagcttttt acctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga
      421 ggggcaactt ggtctagtcg acattatgtc tactaacctt taaaataaca cgttttataa
      481 aataaaccct cttgacaata tttaattttc tgattattct aaatatatac agaaagcgct
      541 ttcctaggat tttatatcta aggaggaagt aacaatgaaa aataaaatta ttaaaattat
      601 tacttgtcta tttttgttcg gtatgattgt tggagcaagt actccgttaa atctgtttcc
      661 ttttagtgct agaggtataa aagtggaagc tgcaagttca cgatataatc ataatcatcg
      721 tggatttact tgctataaaa tgaactatta tgttactaat aaaatgtgta gagatttgaa
      781 aaaaaattat aataagctaa agactcctgc tcaaattgca tcttttattc ctattggtgg
      841 agttggaaca tgggttatta caaatgcttt tagtagtgct gttgatgcaa tgaatgtctt
      901 tgtaagagct gcaaatcaag gaaaaggtgt acaattaaca tataatgctc actttagcaa
      961 tacaacatca tatcaataca atgattacgc tcgttatgta ataaaataat cagaaagtga
     1021 aactatctca tgaataaaaa gattttagct gtaattattt caataatagt tttaatcact
     1081 atgttcttca tatttaggct aattttcaat ataagcttac aaaaaagtat tctttatcta
     1141 attcctatag catttatctt agcaattttt agaagtattt acggaaataa aaactctaaa
     1201 tagctaaaaa taaatcatac taaattttac aaaaagtggc tagcattttc tagccacttt
     1261 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1321 agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc ttgccaaacc
     1381 cttttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1441 ccgtaggacg tactgaaagg gtgggactta tgagcaaatt aatgcaaaat ttggctagca
     1501 aaaaagctaa aaaatatatg ctaataatcg agcgaaataa acactttttc aagtgagcga
     1561 atccgccatg acaaagctga agcagtttat ttcagctttg tcatggcgtg tgccgacttt
     1621 tttaaaagtc taggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac
     1681 gtttgaaacg cctaagtata taaatatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttatact aggactgtct taaaatgtct taaacgaacg
     1861 ctcagagctt ctcaaagggt tccttatgaa cggcttttag caaaggagcc ggaggcgact
     1921 gcgcagtata atgtgagagc agagcgatca caccttttta gcgaaccgta gggagcaagg
     1981 tttggaattg gcagttttga taaattcgcg aacctgttta gagaaaaaag gagtgatttt
     2041 ttaggcggga aaattagcct cccctctttt tatatctctt tttatatctc tttttatatc
     2101 tcttttatgt cggctgtgtc cttactctcc caagcaattc agagtttttt caagtaggtt
     2161 ttctacgctt ttcaagtagg ttttctacgc ttttcaagta ggttttctac gcttttcaag
     2221 taggttttcc gacattattg agtattttta taaagtatgt tacttttatt tcatacttta
     2281 taaaagtagg agaagcttat ggcaaatgaa attgtaaaat atgaaaatag attaaatcat
     2341 attcctctga gaaaatttaa tagtcgtgaa atgaacttat ttttttcaat tgcttcacgt
     2401 gtacgtgaaa aaggtgtaga tgagattatt ttttcatttc acgatttgaa aaaactaagc
     2461 ggttatacag aacgtgggga acaatttata aaagatctta gtagcacgta tgacaaatta
     2521 ttaagtatta atgcttggaa tgatgatgga aatactttaa ctaaatttgt tgctttcaca
     2581 aaatatagca ttatacgaga taaacaactt gttataattt cagttaatcc agaatttaaa
     2641 ggacttttta atcaattaaa gaactggaca agatttaatc tagagcaatt tactaatttg
     2701 gatagcacat attcaaaaac tatgtttcga ttattaaaac aatatcgaac tcaaggctgg
     2761 actgaatttt ccaaagaaaa attttttgat ttactagata ttcctaaaag ttattggaat
     2821 agtccgtcaa atattgacaa atttatctta aggccaatta aagaagaact cactccctta
     2881 tttaaaggat taactattcg taaaaaatat ggtaaaggtc gaggtaaacc tgttatcggt
     2941 tatcgtttta cttggaaagc tgaaattaac catgctgacg atttttccaa agggaaacaa
     3001 gaagatttac gtataaaatt atttaatatt gatcacaatg gagaattaac acgagaagaa
     3061 aaatggcgcg ctaaagatcg aatactaaat cttcccttag gcactcatga agctgatttt
     3121 aaacaacaac aacaaactga agaaaaacag gcagaaaaac gagaactttc aaacaaactc
     3181 aaacaagatt tactaggaaa tttacaaaac ttattcgatt aagcttaagg agtatttatg
     3241 gcagataaaa caatcaaaga acttgcggaa gaattaggag ttagtaaaac agcaattaac
     3301 aaaaaagttt ctgacaaaaa tcgaaaactt tggtttgcaa aaaacggaaa caagtttgtg
     3361 attaacgaaa ccggtcaaaa agctataaaa tcaatgtttt tagtcgataa cgcaaactta
     3421 aatcgaaaac tagttggcga aaaatcaaaa accaaaaacc ataaaaacac caaccaagtt
     3481 tccgaattaa aactcctttc tttcctagaa aaacagttaa atcgaaaaga tatacagcta
     3541 caagaaaaag ataatcaaat aaaacaaatg caaaaattac tcgaccagca acaacaactc
     3601 actctacaag cgaaccaaca aattgagaac ctacaagaac agctccattt aacttataca
     3661 gaagaaactc caacaaccga atcaactgcc ttttctgaaa aaaaagcaga caatacacaa
     3721 ccacttattg aaaaaaagtg gtggcaaatt tggaaatcta atagaaccaa tctctaaaat
     3781 tttctagaag ttagttcgga tagtagagga aaaagttgta ctacctgatg gggagatcac
     3841 tcaaattttt atgagaaaga aaaaatagtt tacaactgta aacaatcaat gtttatagtt
     3901 gtaaactatc aaactatatg gtgacttatt gcttaaaata ggttgaaatc tcactcaatt
     3961 tttggtggaa aaacaaaata aattctaatc gattttgttg aaaaagtcat aaaaaaacaa
     4021 atgtgatttt aacaaaattt caaaaaaatc gtttgaaatt tcgtttattt tttttgagaa
     4081 ttttgatcat cgtagggtca ttgtagaatc attatgagat catcgtggga tcatcgaata
     4141 aagtatggga aaggcttgcg gtgcttggta ttaagggcat tctccgcctc ccaggttctt
     4201 gaccattttc tctcaacgtg gtaaaaatcc cacttataag agaaaatggt caagaaccta
     4261 aacggtcggc gaagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca
     4321 aaaaagaaaa attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa
     4381 tttaaacatt tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta
     4441 aaaaaagtgg aggtataacc tatgtt
//
LOCUS       DANJMA010000104         4374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000104 DANJMA010000000
VERSION     DANJMA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(83..1051)
                     /locus_tag="PWU31_002641"
     CDS             complement(83..1051)
                     /locus_tag="PWU31_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1085105.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="PWU31_002642"
     CDS             complement(1498..2904)
                     /locus_tag="PWU31_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL1085106.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="PWU31_002643"
     CDS             complement(3126..3488)
                     /locus_tag="PWU31_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085107.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3830)
                     /locus_tag="PWU31_002644"
     CDS             complement(3492..3830)
                     /locus_tag="PWU31_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085108.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3809..4228)
                     /locus_tag="PWU31_002645"
     CDS             complement(3809..4228)
                     /locus_tag="PWU31_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085109.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="PWU31_002646"
     CDS             complement(4221..4361)
                     /locus_tag="PWU31_002646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085110.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DANJMA010000105         4371 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000105 DANJMA010000000
VERSION     DANJMA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            106..357
                     /locus_tag="PWU31_002647"
                     /pseudo
     CDS             106..357
                     /locus_tag="PWU31_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="PWU31_002648"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="PWU31_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL1085111.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="PWU31_002649"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="PWU31_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="PWU31_002650"
     CDS             1786..1869
                     /locus_tag="PWU31_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL1085112.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWU31_002651"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWU31_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL1085113.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="PWU31_002652"
     CDS             complement(2994..3857)
                     /locus_tag="PWU31_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL1085114.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="PWU31_002653"
     CDS             complement(3859..4131)
                     /locus_tag="PWU31_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1085115.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="PWU31_002654"
     CDS             complement(4148..4357)
                     /locus_tag="PWU31_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL1085116.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT         1352 a          736 c          822 g         1461 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaac cactcacttc t
//
LOCUS       DANJMA010000106         4232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000106 DANJMA010000000
VERSION     DANJMA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(37..1257)
                     /locus_tag="PWU31_002655"
     CDS             complement(37..1257)
                     /locus_tag="PWU31_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL1085117.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="PWU31_002656"
     CDS             complement(1277..1888)
                     /locus_tag="PWU31_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1085118.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="PWU31_002657"
     CDS             complement(1931..2863)
                     /locus_tag="PWU31_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL1085119.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3283..4143)
                     /locus_tag="PWU31_002658"
     CDS             complement(3283..4143)
                     /locus_tag="PWU31_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085120.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1202 a          853 c          693 g         1484 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa acaaataagg gaaacaaaca acacattttt
     3061 gtgtgttgct tatttccctc taactatcgt tttctatttg atggttaacc tacttaaaat
     3121 aaatactgca acgacaaacg gagatcaagc atgctttgta acgtatttta cgataattac
     3181 gactttattt tattactcta tcttgctgcc atacccgtct atccctaatc atctttggtg
     3241 aattttttca aagttagcat aacaaaaata agtatttttt ccttagttaa tcaatagtat
     3301 catttttttc atatgattta attcatcaat taattttttg tttttattaa ttttagcaaa
     3361 agctagtgca tcttgaatca attcgagcac ttctgccttc gacttatttt tagcctgtgc
     3421 gttcttcgct aattgggcag cagctcgtgc taagtaataa gtcaaatggt tatccgaaca
     3481 aatatcgatt gcgtaattta gtagtgcatc acttgtttcc aaatcaccgt cttgcgcaaa
     3541 aaaaacacca caatgaaaga cgatatttaa cacccgccag atatcttctg ttgtacgaat
     3601 cggatacgta taaatttttt caaacactcg attgaaataa acttctgctt tttccaactc
     3661 tttttctcta gcatagacca ttccaatacc tgtatatgcc aataaacgaa acaacgtgtt
     3721 ggctgtttct gccatcaaca actgatcaaa atggaacaac acatccgtaa ttggtgcatt
     3781 cgtaaaaatc atttcaaatc cttttagata gtagtaacgt gcaatcagct cagactcgtc
     3841 aattaccttg ggatcaatgg ctgctaaaat ctgtgtcaat tgttcatatt cactcgtaat
     3901 cagtgaaaac tctgcttttt ctagctgttc atcaagttgt gtattgatca cgttcatcga
     3961 agaaaaaagt tcaactaacg gaagattgat tcgattacat aatttgatta aaatttttaa
     4021 cgaagggact tgaccattgt tctcaaattt acttaatgtt gcttgtgtac aaattccttc
     4081 agacacttct ttttgagata agttggcagc ctttcttgcc gtaataaatt tttcaatgtt
     4141 cattattttc tctccaatca gacctcacac gaaaatcatt tctgtctcga ttttagcatg
     4201 ttttctttgg gaatgcttta attggttaag at
//
LOCUS       DANJMA010000107         4034 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000107 DANJMA010000000
VERSION     DANJMA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            73..234
                     /locus_tag="PWU31_002659"
     CDS             73..234
                     /locus_tag="PWU31_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL1085121.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="PWU31_002660"
     CDS             253..1530
                     /locus_tag="PWU31_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1085122.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="PWU31_002661"
     CDS             1540..2196
                     /locus_tag="PWU31_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1085123.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="PWU31_002662"
     CDS             2196..3572
                     /locus_tag="PWU31_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1085124.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="PWU31_002663"
     CDS             3702..>4034
                     /locus_tag="PWU31_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1085125.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          439 c          704 g         1279 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaattaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DANJMA010000108         4000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000108 DANJMA010000000
VERSION     DANJMA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            5..1150
                     /locus_tag="PWU31_002664"
     CDS             5..1150
                     /locus_tag="PWU31_002664"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL1085126.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLATSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="PWU31_002665"
     CDS             1321..1779
                     /locus_tag="PWU31_002665"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL1085127.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="PWU31_002666"
     CDS             1938..2408
                     /locus_tag="PWU31_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL1085128.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            2541..3968
                     /locus_tag="PWU31_002667"
     CDS             2541..3968
                     /locus_tag="PWU31_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HDL1085129.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
BASE COUNT         1342 a          727 c          734 g         1197 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagcgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct cagactgtag
     2461 acaaactcct aaattgggag tgttgtttgc agtttttttg ttttatactc attgtataaa
     2521 ctattctggg agtgattcta atgatgagaa aacaatcgat tgaaggtaga aatcaatttg
     2581 ctatgttaac aattgatgat cttgtaccta aagatcactt agttcgaaag attgatgctg
     2641 cgattcagtt tgatttcatt tatcccattg tagaatctac gtattcgacc catggtcgtc
     2701 ccagtattga tccagtggtc ttaattaaat tagtatttat ccaatattta tttggtatcc
     2761 gttcgatgag acagactatt aaagacgttg aaacaaatgt tgcttatcgt tggtttttag
     2821 gttattcatt cgaggatcct atcccgcatt tctctacttt tggtaaaaac tatgttcgaa
     2881 gattcagaga aacaacttta tttgaagaca ttttctctca tattctagaa caagctgtaa
     2941 aagctggctt cgttacggaa gacaaccttt atattgattc tactcatatt aaggcaaatg
     3001 ccaataaaca taagttcaca aaagaaatga cctacggtca agctaaagct tatcaagatg
     3061 aattagaaga tgaaataaat aaagaacgta ttgctgtagg aaaacgtcct tttacatggg
     3121 acacagaaag tgagttgagt cttcaaaaaa taagtcattc agatccagaa agtggttact
     3181 atgttaaagg tgaacgagaa aaacaatttg cttattccgc gcatacttcc tgtgaagata
     3241 atggttttat cttatctaca ttaatcacac caggaaatgt acatgacagc caagtcgcaa
     3301 tcgatctggt caaacagtcg aaaagagctt ttccaaatat aaagcgtgtt gtcgctgatg
     3361 caggatataa gacgcctaaa tttgttcatt ttctgtcaca cttgaaactc tacccgattc
     3421 ttccatatac tgtacctcat ggagtaaaag gaatgtttcg taaaaaagac tttgtttatg
     3481 atgcctattt tgactgctat ttatgtccca ataatcaacc gttgttattt tcaactatca
     3541 ctcgtgatgg ttatcgaacc tataaatcaa atccaaaatt ctgtaggagt tgtccattac
     3601 ttaaaaactg tactttgagt acaaaacatc aaaaaatagt aactcgacat gtttgggcgg
     3661 atcttatgga tgaacttgaa caccaacgcc atacggcgtt gaatagagaa atttataaaa
     3721 agagaaaaca aaccattgaa cgaatatttg cagatgcaaa agaaaagcat ggtatgcgtt
     3781 ggacgaaata tcgaggtctc gaaaaagtcg ccacgcacac gatgcttacg tttgctgcca
     3841 tgaatttaaa aaaactagcc acatggctat ggaaagcaaa aaagcctatg gctttttatt
     3901 taaaatttct acaaagacta acaaaaagga gttggcaatt aagccactcc tttttgtcta
     3961 cagtctgaga tgaactattt ctagttcatc tttttgcctt
//
LOCUS       DANJMA010000109         3910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000109 DANJMA010000000
VERSION     DANJMA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            118..3150
                     /locus_tag="PWU31_002668"
     CDS             118..3150
                     /locus_tag="PWU31_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1085130.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3340..>3910
                     /locus_tag="PWU31_002669"
     CDS             3340..>3910
                     /locus_tag="PWU31_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL1085131.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1344 a          622 c          827 g         1117 t
ORIGIN      
        1 ataataaatc aaatgattga taataagata gctgaattga tggaacttga gagttataaa
       61 aaagctctca tctacgaata cgtaaccggt aaaaaagaag cctaggaggt gattcatttg
      121 gtagagaaca aagaaattcg ttttgagcag gatattgaga cttcactgac tacgtatggc
      181 aaatggaaga aagaaacatt caatgaatca aactttgatc ccataattgg tttggattgt
      241 caaaaattgc taggctttgt ccagaagact caaccaaaac agtgggcacg tcaccaacga
      301 aactataaca atgatggaga agtcaagttc cttcaacgct ttaatcaaga ggtagaaacc
      361 cacggcatgt tacatgtatt gcgcaatggg atcaaagacc gcggtgtgcg ctttaaagta
      421 gcttacttca aacctgcttc agagctgaat gatgaaacga tggaaaagta tgaagcaaat
      481 agttttacct gtactcgcca gtttgcttac agtcctaata accataaaac cattgatatg
      541 gttttgtcgt taaatggtat tccgctcgtt gctttagaat tgaaaaacca gtacactggt
      601 caaacagtag aaaatgccaa actccagttt caaaaagatc gtgatccaaa agaattggta
      661 tttcgtttta atcgccgctt cttagtctat tttgcggtgg accatacaga tgtttatatg
      721 acgacaaagt tggacggtaa aaaaacgtac ttcttgccat tcaatcaagg ttccaatggg
      781 ccgggaagag ttggtggagc aggcaatccg tccaataaag atggctaccc aactgcctac
      841 ttgtgggaaa aggtgcttac tagagacaat ttgatggata ttatccaacg ttttatgaat
      901 ctagagatta aaaaagaaaa gcaaggcgac agagaagtca caaagaaaac gttgattttt
      961 cctcgttatc atcagttaga tgtggttcga aaattggttg cagatgtaaa aagccatggt
     1021 agtggagaaa attatttgat ccagcatagt gctggttctg gaaaatctaa tagtattgca
     1081 tggctggcat accatttagc ctctattcat gatcaactga actacccaat tttttcatca
     1141 attattattg tgactgatcg aacagtgttg gatcgccagt tgcaaaatac tatcatgaac
     1201 tttgatcatc agacaggttt ggtagaaacg attgacgatc gcaaatcctc tcaagattta
     1261 aaagaagcca ttaatgatgg gcgtaagatc attattacca ctttgcaaaa gttcccagtg
     1321 atctatcagg aagtagatca agtggaaggt cgtaattttg ctgtgattgt ggatgaggca
     1381 cattcttcac aaactgggaa cagtgcccag aagctaaaag ccgctctttc ggacaaatct
     1441 aaatccttgg aagagtatta tgaactggaa ggcgaagttg acgatgtatt accagatgct
     1501 gaagatgaac tagttcaaca attggtagca caaggtaaac atcaaaattt aagtttcttt
     1561 gcttttaccg ctacaccgaa agaaaagaca ttggaaatgt ttggtacgaa acaaccagat
     1621 gggcatttcg aaccctttca tgtttactcg atgcgccagg cgattgagga aggctttatc
     1681 ttagatgtgc tgaagaacta tatgacctat agtacttcct acaaaattgc ccgtgaaatt
     1741 gaagataacc cagaattacc aaagagtgaa gcaacccgag cgattgttcg tttccaatca
     1801 ttgcatcctt gggcagttac tcaaaagacg gctgtgatgg ttgagcaatt ccgagcggtt
     1861 actcgtacag ctattggtgg gcaggcaaaa gcaatggtcg ttaccgcatc acgcttacac
     1921 gctgttcgct atatgaaaga atttaaaaaa tatatagcga acaaaggtta taatgacttg
     1981 gatgtgttgg ttgctttttc tggttcggta gtagatggag agactgaata tacagaaacg
     2041 aagatgaatg tcacaaaatc tggtgagaat gttcgggaga atcaattaaa ggaagctttc
     2101 catacagata attttaatat cttgattgtt gctgaaaaat atcaaacggg ttttgatgaa
     2161 ccgttgctgc ataccatgtt tgtcgataag aagttaaagg gtgttaaagc tgttcagact
     2221 ttatcacgac tcaatcgaac aatgcctggg aagaaggata cctttattct tgattttgta
     2281 aatgatgttg aagatattga agctgctttc caaccctttt atgaacaaac aaatttgaga
     2341 gaagaaatca atgttaactt gatttatgat actcaaagca agctacgaaa atttaatgtt
     2401 tacaatcaag gtgatattga acgtgtcatg aaattggtga aacaagccca aaagcaacag
     2461 aatgagcgct tgcttggacg aatttctagc atgtttaagc cgattatgga tcgctatgag
     2521 gaaatgaaca aggataatca gtaccagttt cgggtgactc tacgtaactt tggcaagtgg
     2581 tatgattata tttcacaact tgatcggtta ttcgatatgg aactattgga ggaacgaatt
     2641 tttactggat acttgctgaa gtttctacct aaagaaacac gagaaaatat tgatatttct
     2701 gacaaagtaa agttggaata ttataagtta cgtcaggact tttccggtga aatccatcta
     2761 gtgaataaag aaggcgagct agtaaatcca aagtcaattg atgcaggaat caaaccgccg
     2821 gatgaacgag attcactaga agaaatcatt cataaaatca atgaacgatt tccaaatgat
     2881 tttgacgaag gagaccgggt gttagtagag attttgtatc ggtcatttgc cgataaccca
     2941 gatttgaagg ttgtcagcat ggctaagaat aacgatgctc agatgtttga aaaatcgctg
     3001 ttccctgatg tatttaaaga taaggtgatg gagcagtacg agaagaacaa tgcagctttt
     3061 gagaaaatgt ttgggcatga tgataagtac tttgatctag tatatacatt agttgctaga
     3121 gatttataca agttattacg gacgaaataa aatttataaa taaggattag acaatacaat
     3181 ttctctcaaa aataaaggag aagttgtatt gtttgctttt aagaaagccc tgaaaaattt
     3241 gttacgtact aaattgtctg aactctgtca tatcagctat gtcagaataa aggaaataga
     3301 tttattttta taaacagtgt cgtctcggag atttaaacta tgtcaacgac aattgaagtg
     3361 aacaaacaaa gcgtgaagca gtttcttgaa acagggaaaa tcaaaaaatt cgttattcca
     3421 gaatatcaac gtccttatgc ctggactgac gagcaaaacc aagtactatt tgatgattta
     3481 gcggagtaca cagcaaacaa taacgagagt acttactttt taggaagcat tgtggcttat
     3541 gaaaatgatc ataacgagca agaaatcatt gatggacagc aaaggataac tacgctattt
     3601 ttatttttga gagctatcta tgctaaatta gaaaatagtt gtgaaaagga agctttattc
     3661 ttgaaaagcc aaattgaacc cgcgttatgg gaacaagatg atttaacagg tgaggttaaa
     3721 ccagataaaa ttctgattat gtctcgagta atgggggatg aggggaatga agaatttgct
     3781 agtattctag taagtggtga agcagatgtt aaatcaaaaa gtaattattc gaagaactat
     3841 attttaattc aacatctatt aaatgagtat gcaacaaacg aaccactatc attttatcgt
     3901 tttatcagta
//
LOCUS       DANJMA010000110         3889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000110 DANJMA010000000
VERSION     DANJMA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..316
                     /locus_tag="PWU31_002670"
     CDS             <1..316
                     /locus_tag="PWU31_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HDL1085132.1"
                     /translation="LTIPGAMSPTEIFKMFEMGADIVKVFPANCLGPEYFNQVQAPLG
                     SLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKSAVHQLNYERLMELAESFIDSLR
                     VE"
     gene            complement(710..1717)
                     /locus_tag="PWU31_002671"
     CDS             complement(710..1717)
                     /locus_tag="PWU31_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1085133.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2184..3590
                     /gene="lacG"
                     /locus_tag="PWU31_002672"
     CDS             2184..3590
                     /gene="lacG"
                     /locus_tag="PWU31_002672"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL1085134.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3613..>3889
                     /locus_tag="PWU31_002673"
     CDS             3613..>3889
                     /locus_tag="PWU31_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1085135.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLREL"
BASE COUNT         1282 a          579 c          757 g         1271 t
ORIGIN      
        1 tctaacaatt cctggggcca tgtcgcccac agagatattc aaaatgtttg agatgggtgc
       61 tgatattgtt aaggtgttcc ccgccaactg tcttggaccg gaatatttca atcaagtgca
      121 agccccttta ggaagtttac ctttgatggc tgtaggaggg gtagatcaga ctaatgcaca
      181 aaactatttg aacaatggag cctcttatgt aggaattggt tcaaaatttt ttgaaaaaag
      241 tgctgttcac caactaaatt acgagaggct aatggagttg gctgaatctt ttatagatag
      301 tttaagagtt gaatagaacc catttgcgga atacaacttg ttttataata tggcgttatc
      361 tgtataaaac atagtatcac agtgaatgag attgaactgg tagattgatt tttcgaccag
      421 ttctttttgc gcgctctgat gcttgatata ttcccaacga gataaaaaat cccccaagat
      481 actaagatga agcgtttatc caacctcata cttagataaa aaggaggaaa tgttctgaat
      541 gataaacaaa aattagcgcg cacaatttgg cgatgaaaaa atcatattgt atttacgtca
      601 aaatatcacc atcaaattat ttatggaaaa cataataggc ataaacccga ttgctcggct
      661 actatgtgga cacaattata gttttaaaga taagtttcaa ttacttattt tacacctctt
      721 ctttagggat aaaacttttc ctaacggtca attttgcgcc gattaatgtt ttctttgata
      781 tgtcccgcga atataccagt tgatcactta ataaatctac acaggtttta gctatctctt
      841 cgatatctat cttaaaagtt gtaagtggtg gtgcaacgaa ttttgcgatg tcgtcatcat
      901 taatacttat tactgccata tctttaggga tgttaatacc caattcatgg cagccctgaa
      961 gaacgccggc agctatagtg tcagatggta tgaaacatgc ctcgggcata tcttcattta
     1021 actcagcttt cattttaaga gttaattgat acccttcgtc aacagtaaat gatccttggg
     1081 agaaaatata cttttcattg agtagtccgc tcttttctaa atgatatcgg aaggcatatt
     1141 cacggctatc aatctcttct ttacccgttt ctaggttaaa gaaatttcct ccaataaatc
     1201 caatttttgt gaaaccttca ttaataaagt agttaattgc ttgtctggcc atttcgtgga
     1261 aatcaggttc tacagtatcg aagtgttcag gtaatgggtt tattcctcgg attaccccct
     1321 tatacccttt ttgatttaag tagtacaatt ccttttttgt caattgtcct attgcgataa
     1381 atccagtaat atcgtcagga atgcttttta caccatcgtt tttagagatg aaaatcaaat
     1441 ctagattagc ttttttacta taatttttca aatcatcttt gacatgttga tagtagacat
     1501 ccttttttaa ctgttcctct gagatccaaa aaagaatggc aatcttgtac agagtagtct
     1561 taatttttgt cagatcatat cctaaagatt tggctgtaac gattattttg tttttagtat
     1621 cagatgtaat agagagtgac tcatcacctt taaaaagtct tgatatagta gccgttgagt
     1681 aaccagattt ttttgcaata tctctaatag taaccattgt tcatcctcct agggatataa
     1741 taatcctttt ctttgttaaa gtaaatcgaa actgctcttt tagtaactta aattactttt
     1801 tctagcagga aacgaaactc tgatatcagc agagagccta atccattaac agagaagatt
     1861 gaatcgataa tggatagcca cttggagaat gattagtagg tgggattgat tttagtggga
     1921 atcagtccgt tttcttgttt cgtgtaaatt aaattagtaa aaagataaat ttattttaca
     1981 aaaaaacgtt tacaaaacgg tttttgaatg gtatatttat tttacatcaa gggatataag
     2041 tgcttgtata tcgcaataaa atagatattc gaggtcggtt tttcgttgtg taaaaaaatt
     2101 tacttataca tgttaaaatt atgctgaggt gatttatggg ttattggcat attgatcaaa
     2161 ctaattttca aggagagatt aaaatgaatt ataaaacttt accagaagac tttattatgg
     2221 gaggagctac ggcagcttat caagcagaag gctctacaaa agaaggtgga aaagggcaag
     2281 tagcgtggga tactttcctt gtagaaaagg gacgctatac tgctgatcca gctagtgatt
     2341 tttataatca atatccagtt gatgctgaat tatgcaaaaa atttaaaatt gatagtatta
     2401 ggatgtctat tgcatggagc cgtatttttc caaaaggcta tggggaagtg aaccaaacag
     2461 gagtggaata ttatcataag gtatttgctg aattgaataa gcatggtatt attccttttg
     2521 taactcttca tcattttgat acccctaaag aactatttga taatggagat tttttaaatc
     2581 gagaaaatgt tgaacatttc gtaaactatg cacagttctg tttcgatgaa ttttctgagg
     2641 ttcattattg gagtacattt aatgagattt atccagttgc ttcaaatcaa tatttgatcg
     2701 gatcgtttcc tccagaagtt caatttgata tagagaagac tgtaacttgt ttacacaaca
     2761 tgatgtatgc gcacgcaaag acagtaaaca tttttaaaga tggtggatat aaaggggaaa
     2821 ttggtgttgt tcactcgcta gagacaaaat atccagcaag tgacaaagaa gaagataagc
     2881 atgctgcgaa attagatgat gcgttatcaa ttaagttctt attagatgct acttatttag
     2941 ggtattactc agatgaaaca atggcacata ttaatgagat tttagcagca aatgggcaat
     3001 ctgcagatat tgattctaat gattttagag agatgaaaaa agcagcaaat cgaaatgatt
     3061 ttcttggtat caataactat ggtagccatt gggttaaaga gtacaaagga gaaagcacac
     3121 ttcactacaa cactaccgga gaaaaaggta gccaagtcta ttgcattaaa gggattggcg
     3181 agagaatcgt gcgtgaggat cttccgaaaa ccgattggga ttggatcatt tatccaaaag
     3241 ggttatatga tttacttgta cgaatccaag acgaatatcc aaattataat aaaatataca
     3301 ttactgaaaa cgggctaggg tataaggata attttgaaga tgggattatt ctagatgatg
     3361 atcgaatcga ttatttacgc cagcatttta atgcgatttc cgatgctatc aaaatgggag
     3421 ttaatgtaaa ggggtatttc atttggtcat taatggatgt tttctcttgg gttaatggat
     3481 acactaagcg ttatggacta ttctatgtag atttcgatac acaaaaaaga tatccgaaag
     3541 agtctgcata ttggtataaa tttgttagcg aaacaaaaac gataatataa aaatagttag
     3601 gagggcaagg aaatggatag gaaagaattg caattattag gatttgaaat tgttgcttat
     3661 gcaggagatg ccagaagttc attattaaca gtgttgaagg aagtgagaga aggtaatttt
     3721 gataatgttg aacgattatt agcggatgct gatgaaaatt taaccttagc gcataactca
     3781 caaacgaaat gtttagctga agaagcgtca gggaacgaaa tggaagtagg cgtaattttt
     3841 attcatgggc aagaccattt aatgacaacg attctattaa gagaactta
//
LOCUS       DANJMA010000111         3872 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000111 DANJMA010000000
VERSION     DANJMA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..391
                     /locus_tag="PWU31_002674"
     CDS             <1..391
                     /locus_tag="PWU31_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDL1085136.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="PWU31_002675"
                     /pseudo
     CDS             388..945
                     /locus_tag="PWU31_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            942..1238
                     /locus_tag="PWU31_002676"
     CDS             942..1238
                     /locus_tag="PWU31_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085137.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="PWU31_002677"
     CDS             1315..1728
                     /locus_tag="PWU31_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL1085138.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2037..2189
                     /locus_tag="PWU31_002678"
     CDS             2037..2189
                     /locus_tag="PWU31_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085139.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2186..2461)
                     /locus_tag="PWU31_002679"
     CDS             complement(2186..2461)
                     /locus_tag="PWU31_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085140.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2915..3121
                     /locus_tag="PWU31_002680"
     CDS             2915..3121
                     /locus_tag="PWU31_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085141.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="PWU31_002681"
     CDS             3317..3484
                     /locus_tag="PWU31_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL1085142.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3510..3854
                     /locus_tag="PWU31_002682"
     CDS             3510..3854
                     /locus_tag="PWU31_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1085143.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1341 a          600 c          829 g         1102 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctaagaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
     3601 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     3661 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     3721 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     3781 atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac ttcgatgcaa
     3841 gtgaaaggtg gtaagtgcat gatcatcaat ga
//
LOCUS       DANJMA010000112         3865 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000112 DANJMA010000000
VERSION     DANJMA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(248..469)
                     /locus_tag="PWU31_002683"
     CDS             complement(248..469)
                     /locus_tag="PWU31_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085144.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="PWU31_002684"
     CDS             complement(567..812)
                     /locus_tag="PWU31_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085145.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="PWU31_002685"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="PWU31_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="PWU31_002686"
     CDS             complement(2036..3037)
                     /locus_tag="PWU31_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL1085146.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="PWU31_002687"
     CDS             complement(3196..3462)
                     /locus_tag="PWU31_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085147.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="PWU31_002688"
     CDS             complement(3452..3808)
                     /locus_tag="PWU31_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1085148.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1433 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaat
//
LOCUS       DANJMA010000113         3858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000113 DANJMA010000000
VERSION     DANJMA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..101)
                     /locus_tag="PWU31_002689"
     CDS             complement(<1..101)
                     /locus_tag="PWU31_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1085149.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQE"
     gene            176..454
                     /locus_tag="PWU31_002690"
                     /pseudo
     CDS             176..454
                     /locus_tag="PWU31_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            597..818
                     /locus_tag="PWU31_002691"
     CDS             597..818
                     /locus_tag="PWU31_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085150.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            834..1676
                     /locus_tag="PWU31_002692"
     CDS             834..1676
                     /locus_tag="PWU31_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085151.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1739..3541
                     /locus_tag="PWU31_002693"
     CDS             1739..3541
                     /locus_tag="PWU31_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDL1085152.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3600..>3858)
                     /locus_tag="PWU31_002694"
     CDS             complement(3600..>3858)
                     /locus_tag="PWU31_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL1085153.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1366 a          560 c          772 g         1160 t
ORIGIN      
        1 atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac ggctacaata
       61 atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc tcctgctatc
      121 tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca gaaccaaaga
      181 actcatcaag ctggctaata agggtcaaga ttatgaacat ctagcagatg agattgatga
      241 gctgagagac aagcgacagc tccttttagt agaagatgcc tccctcagtg gcgagaacga
      301 gcgaatcaat gagctgattg aatttatccg caagaacaaa ttccgtacct tagagtacga
      361 tgataagctt gtaaggaaga taatccagag cgttacagtc tatgaagaac acttcgtcat
      421 atccttcaaa tctggcatcg aaatggaaat atgaaaacca gaagtaaata gcccatgatt
      481 ctacagtaga gtcgtgggtt ttcttttgct tgctgattat aataatattt taataactat
      541 tgttattata aaccaaatga tttataatat tcttagcgta aacttggagg tgctgtatga
      601 ctacggcaga aatgattaaa gaactgtgtg agcaaatgaa tataagtgtt tccgaacttg
      661 ctagacgtat tggacagact ccacagaatt tcaataagaa attacaacga gaaacggtaa
      721 ccttagatga gttgaaagcg attgctgatg tgctaggtgt caagtttgtg caggctttta
      781 tattgcctga tggcaatgaa ataaagatag gtaattagga ggtgtaattt gtattgaaaa
      841 tcataacaat aaatgatgtt gagtatgctg tatttgctgc taatgaaggt acttccaaac
      901 cacaacctca tatcatagaa acgaaatctg gtactattcc agaaggaaaa caactgagtt
      961 tattaaaaga atatcttaag cagaatgata tttccccaat aaaaggagca acaacatatt
     1021 ggtgcattga caaggttctt aagcttgatt caagtaaaga aaaaactata agtgaaacaa
     1081 tccataaaca aaaatactta tctttaactg aagaaaatat tgagaaacag cataagtttg
     1141 taggtgcaag ctctaattat gggaaagaag gtctaataat acatgatgtt ttgaatgcat
     1201 ttcctcttca taatgattta aatactattg cgatgaagat tgcggtaatt gatgtaacaa
     1261 attcaacaca tctttctcaa tataagagca ggttgtctct atatgatttg gctaaagtaa
     1321 ttctggagat tccaaatttt gatgatcggt tggcaaaagg agatcctcag ttgataaata
     1381 ttatcgcaag gaacattggt gcagttaata tgttctcatt tgcatccaag tactgtacat
     1441 atcataatgt ggaagtgtgt gggagagatg attactccat ctttgatggt atcgttaaaa
     1501 atactcttcc acactatatc caaggactaa ctacgaataa gattgatacg tggcgaagaa
     1561 gttttgatta cgaggcattt aacgaatgtg ttggaaaact acttgatgaa aataatatac
     1621 atattccttt tagaagaaga aagttagatc acttcttgtg gtatgcgaat aggtagaata
     1681 taagaaaaag gcatcgtaaa agtgattctg gataaaatta atgggggagg aaataatagt
     1741 gggattattt gattttctaa agaaaaaaca aggaaaggtt agtaaacacg ttgattatca
     1801 actggcttct agtctatttc gtactggtaa agtttttgct gaaaaattgg aagtggacat
     1861 atcaaatgta gatgagtttc gtaaaacttt tatcgctttt gatgtggaaa caactggcct
     1921 aaattcttct tcagatagaa ttgtagagat tggtgcggtg ctatttaaag aaggagttcc
     1981 aattcaatca ttcggaactc ttgtcaatcc aaaagttaga attgcagcct cagcttcggc
     2041 aataaataat ataactaatg aaatgctaaa ctctgctcca aatgagcagg aagtttaccc
     2101 ccagttggta gaatttttag gagatgcact agatggaaaa atcactatgt gtgcacacaa
     2161 tgcaagtttc gattttggat ttttaagtaa cacattttct agacttgggt atgatgctaa
     2221 aattaagtat gttgatactc tgagtctctc tagaaaacat ataaaaggca ttgtaaatca
     2281 taagcaagag actgtagcgg aatatttcgg attaataaat gacactgccc atagagcgga
     2341 atcagatgca gatatatgcg gaaagatttt ttggggtgtt ttagatatta ttgaggagtc
     2401 gttggaagaa caaaaaaagc aaatagaaaa gatgactcca gacataaaag aacttgaagc
     2461 ttgtgctttt attcaagata ttattgagaa aaaaggatgc gacaccagtt ggatcaggta
     2521 tcgaaaaaac agtaataatt atgtcgatgt aacttgtcta tatacattct taaagtttaa
     2581 gtttgctaag aagggaaatt acatcattat tagaaaagat gctacaaatg gaattgattt
     2641 acctatagaa ccttgtactg taagtgaagg cggtacaaca aatgtgcgag tctattttcg
     2701 tagccccttt gacttaacgc ctctttcaaa ttatattttc agtgcttata gcgattgtta
     2761 taattctatg aatgaatata tgagtgttag taattacact aaaaaagaag cagagcaaag
     2821 ccttagtatg ctgaaatcta tttcaaaatc agcgatgaaa gagttattat tcgatgcaaa
     2881 aaatcgtgga tatgatgaga ctgaagttaa cctaaatatt gaaaaagtga tatctagaga
     2941 agatgtaatt attaacgggt tgcataatcg agtttcatta aacaagatac gaaatatagg
     3001 gaattgggag aaaggatttg atgcaggtta tccatattgg gaacgtggag aaactgctag
     3061 gaaaaatggc aaagtagatg aagctattgc actttttgat aaagctcgat ataacggcta
     3121 tgatgctcct gcgctatatg attcttatgc taaagcttat cgaaaaatta aggattacga
     3181 taatgaaatt ctcattcttg atgaaggaat tatgcgtaaa acacggcacg atgttggtac
     3241 tttagaggct cgtagggata aggcaattaa gctattattt gctaaacaag aggcagagcg
     3301 gatagctaag gaaaaatctg attttctaaa agcgaacaaa aaagaggatg cgattagtga
     3361 gcctagcaag aagcgtggaa gacagattat tcaaatgact gatgatggaa caataataaa
     3421 agaattcgat actatagctt ctgctgtaaa agaagttggc gttaactcaa aaagtattag
     3481 ggatgctgca aatggagttc aaaaacatgc tggaggatac tactggaaat acaaagatta
     3541 gcagataatc taagataacc taggatcttc ttaatagata cccgtggtgc tagttaattt
     3601 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     3661 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     3721 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtcccaac tgtttttctt
     3781 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtggcgga
     3841 atggatatag taatgccc
//
LOCUS       DANJMA010000114         3720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000114 DANJMA010000000
VERSION     DANJMA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            4..1960
                     /locus_tag="PWU31_002695"
                     /pseudo
     CDS             4..1960
                     /locus_tag="PWU31_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
     gene            1950..3545
                     /locus_tag="PWU31_002696"
     CDS             1950..3545
                     /locus_tag="PWU31_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1085154.1"
                     /translation="MKNKWCKSAVIYQIYPRSFQDTNGDGIGDIPGILSRLDYLENLG
                     INAIWLSPVYESPNDDNGYDISDYRKIGAEYGTMADMDLLIKEARQRGIRIIMDLVIN
                     YTSDRHPWFQLAKKSVTSPYHDYYVWRKEPNGLESNFGGSAWTYVPEVNQYYLHLHSK
                     KQPDLNWQNPKMRQAIYDMMNFWLEKGIGGFRLDVIDLIGKQPEQSITKNGPLLHDLL
                     QEMYQKTFGHYDVVTVGETWGATPKIAQLYSKEERKELSMVFQFEHINLDKQNGKRKW
                     DLKDLDPQELHRTFSKWQIELGGCGWNSLFWNNHDLPRIISRWGDDQEYRTISGKMLA
                     IYLHFMQGTPYIYQGEEIGMINYPVKSIDEVDDIESQRMYAERIKKGYSKEELIQSIN
                     SKGRDNARHPMQWNNHKNGGFTEGTPWLPVGNTEEINVEAALAAQDSLFYTYQKLIEL
                     RKELPVIVYGKYQVIETEEPNVLAYLRSLKKQKILVVVNFSDQKTTFPIGKYRVKQVL
                     IQNYKKKITNNLLPYEAFSALIY"
BASE COUNT         1187 a          616 c          841 g         1076 t
ORIGIN      
        1 gaaatggatt acaaaaaagt agctgaagaa atcagcaaag cgcttggaga agacaatgta
       61 caagcagctg cgcattgtgc aacacgttta cggcttgtct taaaagattc agacaaagta
      121 gaccagaagg ctctggatga taatgaaagt gtgaaaggaa cttttgaagc aaatgatcaa
      181 tttcagatca ttatcggagc aggtgatgtg aataacgtat atgatgaatt cgttaagatc
      241 accggcgtaa aggaagcttc aaccgacgat ttgaaagcgg tagcatcgaa taagaagaat
      301 aatactgtca tggcctttat caaggtgttg tcagatatct tcgttccgat tgtccctgca
      361 ttggttgcgg gtggactgtt gatggctctg aataatgttt tgacggcaca ggatttgttt
      421 ggtcctcagt cagtggttga gttgtatcca tcagtgaaag attttgctgg catcattaat
      481 ttacttgctt cagcgccgtt tgcttttttg ccgattttgg taggtttttc agcaactcga
      541 cgttttggag gcaatccgta tcttggagca gcaatgggga tggcgatggt catgccggat
      601 ttagtgaatg gatacagtgt gacgaatgct attgcagaag gatcgatgcc ttattggaat
      661 atttttggaa tgaatgtggc acaagccggt tatcaaggat ctgttcttcc agtccttgca
      721 gtttcttgga ttttagctac tttagaaaaa ttcttccata aacggttggc taatgctttc
      781 gactacacat ttactccaat gctagctatt attattactg gttttttaac ttttacagta
      841 gtagggccgg tgatgcgtac tgtcggagac ggcttagctg atggattaag ctggttatac
      901 agtactactg gagctttcgg cttaggtgtc tttggtttct tctattcgcc gatagtcatt
      961 accggattac atcaaagctt tccagcaatc gagactactt tactagcaga tgttgccaga
     1021 acgggtggct cttttatctt tccagttgcc tcaatggcaa atgtagcgca aggagcggct
     1081 gctttggcga ttatgctgct gacaaaaaat aaaaagcaaa agagtctagc taccactgca
     1141 ggtgtttccg ctatgttggg gattactgag ccagcgatat tcggtgtgaa tttgaaactg
     1201 aaatttccgt ttatttgtgg aatgattgct gcaggaattg cttcattctt tatcggcttg
     1261 ttccacgtat tagcagttgc gatgggaccg gcaagcgtca ttggctttat ttccattgca
     1321 cctcgttcga ttccttcatt catgatcgga attgttatca gtatagtgat cggttttttt
     1381 aagcacttac ttctatggga aaaaacatat ggaactgcta gcagatggtg aatcaaaagg
     1441 aacagaagca gctgagtcaa tcagttcagc tcctaaaacg actaggaaac cgcaagatga
     1501 aattttagct gcatcagtca gcgggaagat tctcccttta acgaaggtaa aagatccagt
     1561 tttttccact gaaatgatgg gaagaggtat agcagtcaaa ccagatgacg gggtagtcca
     1621 tgctccagca gatggtatat tgacagttgt ccacgaatct aaacatgcat atggcttgaa
     1681 aacagagaaa ggagcggaag tactgatcca tatcggtatc gatacagtga atctaaaagg
     1741 ggccggattc acgacacagc gacaatatgg tgaattagtg aaaaaagggg atattctcgg
     1801 tacatttgat cgtaaattgc ttaaagaaga agggtacgac gatacaatta tggaaattgt
     1861 cactaattcc tctgcatatg ctaatgtaaa aattcttgaa aaaaacacgg tcaaaagtgg
     1921 tgaagatatt ctgatagtga gtgaaacaga tgaaaaataa atggtgtaaa agtgcagtaa
     1981 tctatcaaat ctatcctcga agttttcaag atacaaatgg agatggcatt ggagatattc
     2041 ctggtattct ttcaagatta gattatctag aaaacctggg aatcaatgcg atatggttaa
     2101 gcccggttta tgaatcacct aatgatgata atggctatga tatcagtgac tacagaaaaa
     2161 taggcgcaga atatggaacg atggcagata tggatttact gataaaagaa gcacgacaaa
     2221 ggggaataag aatcatcatg gatcttgtta tcaattatac gtcagacaga catccatggt
     2281 ttcaattggc aaaaaaaagt gttaccagtc cctatcacga ctattatgtt tggcgtaaag
     2341 aaccgaatgg attagagtcg aacttcggcg gttcagcatg gacctatgtg cccgaagtga
     2401 atcaatatta tctgcacttg cattcgaaaa agcagccgga tttgaattgg caaaatccaa
     2461 agatgagaca agcaatctat gacatgatga atttttggct tgagaaaggg attggcggat
     2521 ttcgattaga tgtcattgat ctgattggaa aacagccgga acaatctatt actaaaaatg
     2581 gtcctctttt acatgattta ttgcaagaaa tgtaccaaaa aacttttggt cattatgatg
     2641 tagtgacagt gggagagact tggggagcaa cgcccaagat tgcgcagctg tattcaaaag
     2701 aagaacgcaa agaattatca atggtctttc aattcgagca tattaatcta gacaaacaga
     2761 atggcaagcg aaaatgggat ctaaaggatt tagatccaca ggaattacat cggacttttt
     2821 ctaaatggca gattgaatta ggtggttgtg gttggaattc tttattttgg aataatcatg
     2881 atctcccacg aatcatttct cgctgggggg acgatcaaga atatcgaaca atcagtggaa
     2941 aaatgctagc gatatacctc cattttatgc aaggtactcc ttatatctat caaggagaag
     3001 agatcggcat gatcaattat ccagtaaaga gtattgatga ggtagatgat attgaaagcc
     3061 aaagaatgta tgcagaacgt ataaaaaaag gctattcgaa ggaagagttg atccaatcaa
     3121 tcaactcgaa ggggcgagat aatgctcgcc atccgatgca atggaacaat cacaaaaatg
     3181 gtggctttac ggaaggcact ccttggttgc cagtggggaa tacagaagag atcaatgtag
     3241 aagcagcatt agctgctcaa gattccttgt tttatacgta tcaaaaactg atcgaattgc
     3301 gcaaagaatt acctgtgatt gtctatggga aatatcaagt cattgagaca gaggaaccga
     3361 atgttttagc atatctacgc agtttaaaaa aacaaaaaat actagttgta gttaattttt
     3421 ctgatcaaaa gactactttt ccaatcggta agtatcgtgt caaacaagtc ttgatccaaa
     3481 attacaaaaa gaaaattact aacaatttac tcccatatga agcttttagc gcgctgatct
     3541 attaagtggt tttttctata gatgaatctt tgttatgagg tagatagttc aatgaaacag
     3601 ttattcgatt tgtttataga aaacaattta atcagcaaaa agggggacaa acttttaatt
     3661 gtctcccttt aattgttagg atggaatatt atcactctta aacaataagt taaaaaaata
//
LOCUS       DANJMA010000115         3701 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000115 DANJMA010000000
VERSION     DANJMA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(75..1169)
                     /locus_tag="PWU31_002697"
     CDS             complement(75..1169)
                     /locus_tag="PWU31_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1085155.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="PWU31_002698"
     CDS             1358..2119
                     /locus_tag="PWU31_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1085156.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="PWU31_002699"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="PWU31_002699"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1085157.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="PWU31_002700"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="PWU31_002700"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL1085158.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         1187 a          721 c          653 g         1140 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt t
//
LOCUS       DANJMA010000116         3481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000116 DANJMA010000000
VERSION     DANJMA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..2232)
                     /locus_tag="PWU31_002701"
     CDS             complement(<1..2232)
                     /locus_tag="PWU31_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085159.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="PWU31_002702"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="PWU31_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          979 a          768 c          549 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtattgcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagttttact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac aacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 a
//
LOCUS       DANJMA010000117         3349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000117 DANJMA010000000
VERSION     DANJMA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            237..1628
                     /locus_tag="PWU31_002703"
     CDS             237..1628
                     /locus_tag="PWU31_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL1085160.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1666..2496
                     /locus_tag="PWU31_002704"
     CDS             1666..2496
                     /locus_tag="PWU31_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1085161.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2468..>3349
                     /locus_tag="PWU31_002705"
     CDS             2468..>3349
                     /locus_tag="PWU31_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1085162.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYNNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSK"
BASE COUNT         1187 a          466 c          643 g         1053 t
ORIGIN      
        1 atatgaatat tgtaacttgt gaataaggaa ttagcaggag aatactagtt cagcataaag
       61 gaagtgatgg gacttctgag acaactttct gttatgcaga aatacacata taaacagaca
      121 ataaacgctt ttccccgttt cttttaattt cctttacaat ttggttattc actaggtttt
      181 taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt gtatatatga
      241 ataaaaatgg ggagtggaat gcggcacgca gaatattcat tattcttatg gatgtacttg
      301 tattaaatgt atcgatctat cttagttttt tgctgaaatt ccagggagaa gtaccagtga
      361 gaaacttaga aacctttcag cattcagcaa tttttatttc catcatcttt attgcgttga
      421 atattttgtt aggagcttat gtattctata accgaatgat cagtgatatt gtcttcatta
      481 ctgttattgg acaagtacta atagcattgg ggatcatggt agttactttt gctggccgat
      541 ggtttgcatt tccgcgtggc gtcattttga tctcgtttat cctaggtacg atattactta
      601 gtgtttaccg gatagtcgtt tacaaactgt acttaagagt cagtcgtgat aagaaagtcc
      661 ttatcgtggg tttggagaaa gatgtcgcac cagctatcca aaattttcaa tctaagaaaa
      721 acaataaaca taaggttgaa gcagtggcaa tcgataatta ttacgaaaat acaaaaaaat
      781 tgatcgatga tatggatatc gtctatttgg cttctaaaat cccagaagat gaaaaaatca
      841 acatttatca attagtgact aaaaaagaga agaaattatt tttaaatacg acttttgaga
      901 atctgacaat gataaatccg aatattatga attttgaaga tgaaagcatc attgaagctt
      961 ctgggttcag aatccaacca gaatacgaac tattcaagcg tctctttgat atcatcgttt
     1021 cgcttatttt actgatcctc gcctcacctt ttatgctaat cacagcgatt ttggtaaaaa
     1081 caacttcacc tggaccaatc atctataaac aagtacgtat tacgaaaaat caaaaggaat
     1141 tttcaatcta taaattccgt tccatgagtg cgacagctga agcaaaatca ggaccagtct
     1201 tggctaaaag taacgatgca cgtgtgacac cagtaggtaa atttatccgt gctgtacgtt
     1261 ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatt gtcggtccaa
     1321 ggccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat tattacttaa
     1381 gacacaatgt acgtgcggga attaccggat atgcacaagt atacggaaaa tatgtatcag
     1441 attataacag taaactacgt tttgatttgt tgtatatcaa aaagtattcc ttactattag
     1501 atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtcc tcacaaggac
     1561 tagatgaaga tgaaaatatc agttcattag cagaaagtgc atttccaaaa gaaaaaacgt
     1621 atcgttagat tggaaaaatt ttgagcaaag aagtaggaga gaacattgga aaataaaaat
     1681 ataagtatac tgctttcttt gtatgataaa gaaaagccgg aatatttaaa ggaagcatta
     1741 gctagtatct ttgagcaaac ggtgatgccg gcagaaatcg tattggttta tgatggccct
     1801 ataaatgaga agcttcaggc agttgtcact tatttccagc aaaaagttcc tcacttgttt
     1861 acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaac tggattagaa
     1921 catactaaaa atcgcttagt ggccagaatg gatacagatg acatcatgga accacacaga
     1981 ttggaaaaac aattagagat cttccaagaa tatccagaaa tagcgattgt tgggagtaat
     2041 attgaagaat ttactggcac acttagcaat gttatcggaa gaaggattgt acctgagtat
     2101 aatgatgaaa tccgtgtgtt ttcgaaaaga agaaaccctt ttaatcatat gacagtcatg
     2161 tttgataaaa atgctatttt agaagtggga aactatcgcc cattaattgg atttgaagat
     2221 tattatttgt gggtaagact attgaaggcg ggatataaag gatataatat ccaagagatt
     2281 ctagtacatg ctagagcagg tgctgacatg tatgctagac gtggaggaag taaatatctt
     2341 ttaccaggaa ttaaaggccg atatcgcgta tggaaagatg gtctaggatc actaaaagat
     2401 tttctgatag ttatttgcgg acatcttgta attagtttat taccgaataa aattagggga
     2461 aaagtatatg aatcaaaact tagaaaaaca aactaaactt tctataatca taccttttta
     2521 caatgcaaag aatactttaa gaagagctgt agacagcgtc ttaacgcaag gttatcccaa
     2581 tattgaaatc ttgttaattg atgatggatc tacagatggt agtagtgaca ttggtgacga
     2641 aattgctaat ctagagagta aagtagcatg ttatcacttg gaaaacgctg gtccatcagt
     2701 tgctaggaac tatgggataa aagaagcaac tggaaatttg gtaggatttt tagatagtga
     2761 cgattatttt ttacctgatt gttttaataa tgttttgaaa gactaccata ggaatcaccc
     2821 cgatatcatt gcttttggat taagtaaagg aaaaaatata gaaaatgcaa atttatttgt
     2881 tcccgaaaag aagattggtt gttcaagtga agaagcaatt gagcaaatgt ttagaagtaa
     2941 ggcagtggac ttttacgtat ggaataaatt ttttaaaagg gaattattca atcatatagt
     3001 ttttccagaa aataaattat atgaggatat ggttcctatg tatgaagctt ttagggcggc
     3061 tagtaaaatt gatattttgc cattttcagg tattttttat tatcaaaata acgatagcat
     3121 cgtttatcag tcttttaatt caaaacagta taataatatt ttgcagagaa aaatattagt
     3181 tgatgaagta aagaaagatt atcctcgact tattaatttg gccaatgctc ggttagtaga
     3241 cggatatctt tcaacagggt ttaaaattac tggcaaaaat caaaatcaga aagaaaaaca
     3301 aaaatattat aatcagtcta aaagagaaat aaaaagtata tattcaaaa
//
LOCUS       DANJMA010000118         3308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000118 DANJMA010000000
VERSION     DANJMA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..208)
                     /locus_tag="PWU31_002706"
     CDS             complement(<1..208)
                     /locus_tag="PWU31_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL1085163.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESE"
     gene            complement(205..498)
                     /locus_tag="PWU31_002707"
     CDS             complement(205..498)
                     /locus_tag="PWU31_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085164.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(536..673)
                     /locus_tag="PWU31_002708"
     CDS             complement(536..673)
                     /locus_tag="PWU31_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL1085165.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            complement(675..1121)
                     /locus_tag="PWU31_002709"
     CDS             complement(675..1121)
                     /locus_tag="PWU31_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085166.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1118..1267)
                     /locus_tag="PWU31_002710"
     CDS             complement(1118..1267)
                     /locus_tag="PWU31_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085167.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1284..>3308)
                     /locus_tag="PWU31_002711"
     CDS             complement(1284..>3308)
                     /locus_tag="PWU31_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085168.1"
                     /translation="GDKGITVTVKPYLNGLQMDTSGGTFTLKGTTPSNRYVDSVATSV
                     TSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQILTTQDIIFFSLGVSDISQGQADEYV
                     SQLEELIRKYNETFDAFMAETKGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGN
                     LQVMYSNSINFGDYDYSGNPNLLSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVE
                     GGVELYYKRRGIANWLPSNKTLVMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVL
                     DLPINSNSITQEWKEFSITGTTPTFSPQAYHPWIQFRAQDGILGEIEMSYDIKIEEGS
                     TATPFQPNLLAEPYNMCREYPNENIADHTVKFPIESGDHQIYQGYTEEELMIGQTYTI
                     TLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVASSPKLFRVYQYP
                     QSTVGACRIDWLKIEKGDTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLAT
                     DIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKI
                     DERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYR
                     PPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
BASE COUNT          893 a          693 c          552 g         1170 t
ORIGIN      
        1 cttcgctttc agccgagaac attttgtagg ttcgatttga tacacccaac acactcccta
       61 aaaacgcgcc aaaaccagta atgatgacaa cacagatatc tgtgtactgc caattgagcg
      121 ctttaccaac taaccccacg aaagtagcta gtgcgggaat aattaccagt gcgaaccatt
      181 ttagtacttc gaacgtttta ttattcattt tcttctctcc ctaaataaag ttttaatttg
      241 ttgcgtgtgt tctaccaatt tttctgcatg tgtatctaat ctttcatcgt gtttctttag
      301 ttcttcatga atcatcaatc gatctgattt gctcgattct aaatctttag tcagcaaatc
      361 taaattgtga cttacttttg aaagagtctc agtaatcttc gagaaagatg cagtaattgg
      421 ttttattact aataaaatca aagaaacgat agcggttatt gatcctgcta tcgctcccca
      481 ttcccctaaa ttaatcatgt gacaactcct tgaatcaaaa taaaaagcac atcaattaag
      541 atgcgctctc ttctttgcta atgattttat ctgcttcttc ttcagtaatg cacaacggaa
      601 cgaaaaccat tacttgttcg ttagtgaaac agccccaatc atacatcatt ttcacatcgc
      661 taaaactaaa catactactc acctcccttt gaagctggat ttagttgctc tttaatttct
      721 gaaatgtctt tgctattttg taacgaagca agcatcattt ttgaattgat ttgtgctaaa
      781 ctatccgctt tttctttcaa tgcagtattt tcctgtttaa ttgctacatc gcttagcatg
      841 agtttggcat tgatctgttt taaatcgccg ttctcatttt ctaacgactc atacattgct
      901 ttgagattgt ttaaatcgtt gtgatctagt gcgttcgcta aaataatcca ttgattcaat
      961 ttaggatcaa acatttgatc agcgattgtt aacggttcgc catcagcacg aattccttca
     1021 agcggtggct gatctgtgta aggaacggat acaagcatgt cgtccaatac ttttcctgcg
     1081 tactctccgc cagtacgtcc atatttccaa atgtttttca tttatttccc tcccagtaat
     1141 tgaatttcgg tttccaattt ggaatcggtg gttcgacttc tgtgcattct tccggtaaat
     1201 gttcttcatc attcacaatg atttgctcga atccgtaagg ttcaattggt ctatatgctg
     1261 ccttcatatc gattcacgct tttctaaatc gtgtaagtaa ttgcaaaggt ataatccgat
     1321 ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga taactgagca
     1381 ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat tggtggccga
     1441 tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt tccgtttaaa
     1501 gccacaaaat atatagaaac tgtttttcct tcacgataaa gctttgctga tccggtattc
     1561 ccgtttgtaa ctgttaatgt ggtagtagct gtatcattaa tgcgttcatc gatcttattg
     1621 tctaattcat ctatagcagt cgcattagca ttcgcttttg tttgagcatc cttagctgtg
     1681 gtgtctactt cattaattga agcagtcaac tgcgaattaa tctccgatac tttcccatcg
     1741 gtataattgt ttgctttacc agtaatttca gaaattttaa tatctgtggc caagttatct
     1801 tcgacatatt ctggtgctag atcccaaaca taatcttttg gattgtttga atcacgcata
     1861 ccagtaccac gatatttata ctcactaata ttcggggttc gtgtgtcgcc tttttcaatc
     1921 ttgagccagt cgatccggca tgcgcctact gttgattgtg gatactgata aacacgaaaa
     1981 agtttaggtg aactcgccac aacattcgtt ggtgtgaatg ttagagacca tacgtctgtc
     2041 aacccatcaa ctggctttag ttctcctaaa cgagcagtcc aatgattata cgctacaaag
     2101 gtttgacttg cgggttttgt tcctttaagc gtgatagtat acgtttgacc tatcataagc
     2161 tcttcttctg tgtaaccttg atatatttgg tggtcgccag attcgattgg gaacttaact
     2221 gtatgatcgg caatattttc gttaggatat tcgcgacaca tgttgtaagg ttctgctaat
     2281 aagttaggtt ggaatggtgt agctgttgag ccttcttcga ttttgatgtc atagctcatt
     2341 tctatttccc caagtatccc atcttgagcc ctaaattgta tccaaggatg gtatgcttgt
     2401 gggctaaatg ttggagtagt tccagtaata ctaaactctt tccattcctg agttatcgaa
     2461 ttactgttaa taggtaagtc taaaacaatc ttgccagttc cactgtcaac atacctatat
     2521 ctaatcagta taagttttcc gtcaactgga ctatagtccg ctccagctct aagcttaaca
     2581 gtcattacaa gcgttttatt agatggtagc cagttagcta tacctcttcg tttataatat
     2641 agctctactc caccttccac ttcagctgat atcttattat atttaaacga gttttcacct
     2701 ttagaaagtg ttccagctga agtattttga ttttcaatta agtcgtatga tagcttactt
     2761 aacaaattag gatttccact ataatcatag tcaccaaagt tgatgctgtt actgtacatc
     2821 acttgtaagt tacctaactt agaaatttct tctttcagag catctaactt gtcttgtagc
     2881 gttttagctt gaccagttaa atcagtaatc tgttgattta agctatccac tctaccttta
     2941 gtttcagcca taaaagcatc aaaagtttca ttatactttc gaatcaactc ttctaattgc
     3001 gaaacatatt catcggcttg accttgcgaa atgtcagaca ctcctagtga gaaaaaaatg
     3061 atatcttgcg ttgttagaat ttgattgtct tttctatatt ctacgtagca gtgtttataa
     3121 tatcctgctt cactcataaa tgtgccatca agagaaaacg tgacttcttc actagttacg
     3181 ctagttgcaa cactatctac gtaacggtta gatggtgttg ttccttttaa agtaaatgtt
     3241 ccgccactcg tatccatctg caagccattt aaatatggtt taaccgtcac cgtaatccct
     3301 ttatcacc
//
LOCUS       DANJMA010000119         3305 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000119 DANJMA010000000
VERSION     DANJMA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(525..1139)
                     /locus_tag="PWU31_002712"
     CDS             complement(525..1139)
                     /locus_tag="PWU31_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1085169.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1745..2122)
                     /locus_tag="PWU31_002713"
     CDS             complement(1745..2122)
                     /locus_tag="PWU31_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085170.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2422..3303)
                     /gene="galU"
                     /locus_tag="PWU31_002714"
     CDS             complement(2422..3303)
                     /gene="galU"
                     /locus_tag="PWU31_002714"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1085171.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1015 a          672 c          501 g         1117 t
ORIGIN      
        1 tttcatttta caatcattga aaaattgaat ttcattgcaa aaaaaataat aaacacttat
       61 aaatatccat tatctatcct tcaatttttg tgtattttat ttctattaga aaaaggtatc
      121 ctgttggaca attttgttat atcaggtaaa cttcattgca ataatgataa caaatgtact
      181 ataattactt tgtaaagagt tttggggaag actctttcat caacgtgtat taagagacaa
      241 gaaagaatcc atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca
      301 ctacatattc ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact
      361 ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa
      421 gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat
      481 gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg
      541 tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca
      601 agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt
      661 aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta
      721 tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca
      781 ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga
      841 agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct
      901 tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc
      961 ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc
     1021 aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc
     1081 tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt
     1141 tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt
     1201 acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa
     1261 aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca
     1321 tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc
     1381 gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat
     1441 tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat
     1501 ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt
     1561 ggtatgattt atatatatat aaataacata aaaaaaggaa tggcttatcc cgatacaaca
     1621 agcattcctt taaaaaagat tcttttatat aagtgaaaaa agcttttttc caccaattaa
     1681 ataaatctct catctcaata gtttatccat tattttatct ttttttgcgt atacctacta
     1741 aatattaccg tcttcttctc gttcttctac tttgttcgag agaatctgta ctagaaaaag
     1801 tagttgtatg tctacctttt ctgcctatcc gagactttct tttcatatat gggatataaa
     1861 tgattagata cagtacagca aaaaataaag caaatacgca agcttcccaa ctacctaacg
     1921 ccaattcaac catcaaggta gcaaccccaa cggatgctgc ataccagata aaaatcagaa
     1981 acgtttcttt ttttctgacc atgcgaaaca tgataaatag actaataagc aatagtggca
     2041 acacaaatat ccaattgctt ccttccatca gccacttgag tatatcaagc agcgactgtg
     2101 caacagcgac taattttccc atagtgaaca cacacctttt tttataatta aaaaattcgc
     2161 ctttttattc tactatgaat cttctggctt ggctttcatt tctcttatgt aatcattttc
     2221 ttttatacaa cttaaaaaaa aattaaacca ttagcataat attttctgaa tctattcacg
     2281 aaagtctttt tctatctttc gttttccacg actcaactga tctagagtca aaaaactaaa
     2341 acgacagttg cgcaaatgac gcacaagctg ccgctttaat aacaaatagc ctttcattct
     2401 cagcaggcca cttatttcca gttaactttc tgttcttagc tttttactca actggataat
     2461 atatgcactt aatgaagtcc caatttccga atgttgaagt ccatattcaa tatttgtctt
     2521 catatatcct aacttatcgc ctacgtcgta tctttttcct ttaaactctc tcgcatagac
     2581 cgcctctgtc tggttaagag tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc
     2641 tggttcttga gtttctaata tctcaaatat tttaggagtc agtagataac gtccgataat
     2701 ggctaaatta cttggtgctt ttcccatttc aggtttttct acaaaccctt ttactctgca
     2761 acgacccttt tcgctatctg ttttaggatc aataattccg tatttagatg tttcattttc
     2821 tggtacacgc atgacggcaa cagcggaagc ttgtgtttct tcataatcca tcatcaattg
     2881 ttgagtcaat gggatatcat ctttcatcaa atcatccccc agcatgacga caaacggttc
     2941 atttcctata aatgcttttg cttgaagcac ggcatccccc agtcctcttg gatgagattg
     3001 acggatgaag tgaagattga cttgagtcgt ttcttcaact aaagcgagta attctgtttt
     3061 ttccttttcc cgcaaattgt tttccaattc gatatttgca tcaaagtgat cttcaatggg
     3121 tcttttttct tttcccgtta cgatcaagat gtcttcgatt cccgaatcca atgcttcttc
     3181 cacaataaac tggatcgttg gtttatccac gattggaagc atttcttttg ccatggcttt
     3241 tgttgccggc aaaaaacgtg ttcctagccc agcggcagga atgactgctt ttttgacctt
     3301 cactt
//
LOCUS       DANJMA010000120         3282 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000120 DANJMA010000000
VERSION     DANJMA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            3..587
                     /locus_tag="PWU31_002715"
     CDS             3..587
                     /locus_tag="PWU31_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1085172.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(663..1856)
                     /locus_tag="PWU31_002716"
     CDS             complement(663..1856)
                     /locus_tag="PWU31_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1085173.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            1920..2300
                     /locus_tag="PWU31_002717"
     CDS             1920..2300
                     /locus_tag="PWU31_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL1085174.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2374..2736
                     /locus_tag="PWU31_002718"
     CDS             2374..2736
                     /locus_tag="PWU31_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085175.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1115 a          538 c          621 g         1008 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag taaagtttct
      721 tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa cgtgatgcca
      781 ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg gtcgtctgaa
      841 atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat tattctcact
      901 ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc tagataagga
      961 atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc aatcagtcca
     1021 aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc agctttcatc
     1081 tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa agtcttggtt
     1141 gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag catggaagta
     1201 aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag aatcacttga
     1261 tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac acgccctcca
     1321 ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat ttttttctcc
     1381 atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct tcgaacgatg
     1441 tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc atgaatcaaa
     1501 atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt taacacacca
     1561 cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg ttcccaatga
     1621 atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc atgtttgatg
     1681 tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc tgctacccac
     1741 aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt tacactttct
     1801 gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc agtcattttc
     1861 gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga gggaagtaga
     1921 tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag ccttatggcg
     1981 cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa gtacaatccg
     2041 gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca gttcagatca
     2101 tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta gaaaaaacaa
     2161 atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt aagatcggat
     2221 ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct gaactagaac
     2281 ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga atttttatgt
     2341 aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt tgctggtaca
     2401 attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat tgatgacaag
     2461 ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa tatacttaat
     2521 ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga attgacaaat
     2581 acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca aaagaatcaa
     2641 agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg ctctgttctt
     2701 ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc gaacaaagaa
     2761 gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac aatgaaatgt
     2821 tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa acggttgtat
     2881 aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa tttatagttg
     2941 accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga tgttatatca
     3001 catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg ataagataac
     3061 tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa cttcaaaaaa
     3121 gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag atttcgaaaa
     3181 acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc tgatatgata
     3241 taaaagttgc tacggcagcc aaaaacgaat cgggaaacac tc
//
LOCUS       DANJMA010000121         3166 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000121 DANJMA010000000
VERSION     DANJMA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            160..627
                     /locus_tag="PWU31_002719"
     CDS             160..627
                     /locus_tag="PWU31_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL1085176.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            902..1138
                     /locus_tag="PWU31_002720"
     CDS             902..1138
                     /locus_tag="PWU31_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085177.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1162..1503
                     /locus_tag="PWU31_002721"
     CDS             1162..1503
                     /locus_tag="PWU31_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085178.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1496..1687)
                     /locus_tag="PWU31_002722"
     CDS             complement(1496..1687)
                     /locus_tag="PWU31_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDL1085179.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            1897..2544
                     /locus_tag="PWU31_002723"
     CDS             1897..2544
                     /locus_tag="PWU31_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085180.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2804..3148
                     /locus_tag="PWU31_002724"
     CDS             2804..3148
                     /locus_tag="PWU31_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1085181.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1163 a          438 c          619 g          946 t
ORIGIN      
        1 agaaaaagac agaatgaagg cagtagcttt ggttgaattt gctgaggcgt ataaatcagg
       61 agaactcatt tcacgtacaa aataaaaaaa gtcggattcc tccgactatg aataatattt
      121 ccgacataag tattatatca taattggggg aatcagagga tggtactttt cgatgtaaag
      181 aaatatgaga caccaagcgc gaaagatgtt gatatggaac gcacaaaaca taatgtcgct
      241 gtgtttcttt cagcatatct atcagctaga tgtagagtag ggcaacctcg agaaccaaaa
      301 gtgacagcat cttattcctt ggttccacct tctacagctg atcatatatt tgaagcagaa
      361 agaatgctga tcgataaaga agaagcacag gaagaatttg agtatctgca taaattgttt
      421 gttcgaggct attcagcgat acagcatccg cataagcctg atgtgactga aaggcgcaag
      481 aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc tcaaaggaat
      541 aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca atttgcttca
      601 tcgttagaac tggttgcttt caagtagcca gtttttacac tttttatacc cttttattac
      661 caatttggtt tccattttat acctttttta taccaatcac ttacctattc aacgttgtat
      721 tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat
      781 ctccctcatc gtttaattaa gcttcgatag gcagcagcgg aaatattaag aataaggatg
      841 tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg tctattaatt
      901 tatgtattgg agggtatata aaatgaagat cgcttccgaa aaaattgatc aactaacaat
      961 tttagtaaac caaagaatcg aagatattat caaagaattc gaaggtgaaa agattaagaa
     1021 cataaagctt actttaatgg atcataagtt cgaattaaat tcgacaaaaa ggaagatcgt
     1081 tgagcttata gaagaagaaa tagctctcca gatcaatgag caacaattag ctaattaatt
     1141 tatgtattgg aggggaaatg aatggaagtc gaacaaataa agtatcaagg tatggatcgg
     1201 gaacggttga ttgatattat tgagcaacaa cgtttagaat tgatccaaaa agaagctgta
     1261 tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt atcattcagt agaaaagtac
     1321 agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt ctcgtcaaga tgtatcgatt
     1381 agtatcaaga cgccagtatt taattctgtt aaatcagaaa agctagaaag tatctcaact
     1441 gctttggtag aaaaagcagc tgaaatgaac tccctagcaa atgaagttag tcgtcttatt
     1501 tagtcatatc gataacttga gagtctttgg tgattccgtt ttttatagaa ttaatggttt
     1561 gttcagcgga atatttagtc aagtatgttt cgctagttgc tactacttca ttgttattgg
     1621 acttaataac gaagtaatat tggccattag ttgcttctct tataacaaag tacaaatttt
     1681 tcaccaccta taatttattt cagcggacca ctcgctgata attaaaatta taagctatgt
     1741 ataaaatttc acaatatcaa tttgtcgctg tggcggaaag ggtagacgca ataaaggatg
     1801 aatgggaaaa gtcaatgagt aattatctga aatttacaag tcccctgttt agttatctaa
     1861 gattatttga taatattatt tctgaggtga tagaaaatgg tgggcgtagt taaatttctt
     1921 gggtttacat ttgatggata tggtacgtta ggcgaatggg taggtattat tttgatagtg
     1981 attacaattc aacaaacatc aaagtactac tggtattcta atagttctaa aattgtatca
     2041 agaataactg aaggaataac caaaaaagat attactgtcg tcggtaaata tgggtttact
     2101 ataactttat taaatgaagg gaaatctgaa accgcaataa agttttgcgg gattataggt
     2161 aaatataaca ttttccaacg ttttatcaga aagcattggt acaaatatat ttacaaatac
     2221 cctgtagtta aaaagcttat tgaaaaatat gtggaccacg aaatatctta tctcaatcca
     2281 acagacctta ttaatcaaga attccagaat attggatact tagaaaaagg tctaaaaata
     2341 ccaattgtaa atgaaagttt agttactgca atggtagcta acataaagaa cagcaagaaa
     2401 ttagaaaaga gatataaaaa atgtaaaaaa ttgaaatttt cctttttatt cttagaatat
     2461 aatggcaaag aatattatca agatatttat gttttgcgag gctcgaactt atataaagac
     2521 attaaaaata agatttcagt atgagatttt ttaaaattcg tagatcactc tttgagtggt
     2581 ctttttattt tgcgtaaagg aggcacgacg atgtatagac cacaatactt agaacagaag
     2641 catgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca
     2701 ataaagagcg atacatataa acggaaggaa agcaatgaag ttattccatt gtatggcaaa
     2761 agaatagcta agtattaaat aagattgcga aaggagatgg gacatgaccg aggaattcta
     2821 tagatggtta ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa
     2881 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     2941 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     3001 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     3061 agttcatggc agatatctta cagcgttaga taaacaagag aagaagatag aaagcttcgc
     3121 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatga
//
LOCUS       DANJMA010000122         3117 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000122 DANJMA010000000
VERSION     DANJMA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            463..759
                     /locus_tag="PWU31_002725"
     CDS             463..759
                     /locus_tag="PWU31_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085182.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            761..1183
                     /locus_tag="PWU31_002726"
     CDS             761..1183
                     /locus_tag="PWU31_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085183.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1202..2794
                     /locus_tag="PWU31_002727"
     CDS             1202..2794
                     /locus_tag="PWU31_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085184.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2815..>3117
                     /locus_tag="PWU31_002728"
     CDS             2815..>3117
                     /locus_tag="PWU31_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085185.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1218 a          475 c          603 g          821 t
ORIGIN      
        1 ggtgtatttc attggtccaa gctaaaaaac tcttagaagc gattttagag ccatttaaac
       61 ccacttaatt atattctcca tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc
      121 cgccgtcttc gttcactagt tgcattaatt tttccaacga tcgttttctt tcgggtgtca
      181 tatttttata ttccaacaat ttttcgtctg ttaaatttgg ttatcgatta cacgtgcagc
      241 actccccccc gaattttaaa gagcataagg aaacgtgacc ccacttttaa aaaaagtggt
      301 tggataggct gtgcctatca cgtttctgaa aaaaataaat gaccccaaaa taccaatata
      361 taggatagta caaggatagc aaaggatagt aaaaggatag aggtaggata gcgaaaagga
      421 taggatcctt ttcactatcc tatattatat tggaggatat ttatgaatgt taaaattaga
      481 gatttagatc caaaattcat ttctgaaatt gatcgaagat gcgttgaact aagtaatcgt
      541 acaggaaata aatggagtcg taatgattat ttaaagctac tcgttgaaaa tgattttgat
      601 cgtcctttga tggaatacaa acaagaaaag tttgaccagt tattagaaaa gttttcagaa
      661 attcaaagtt ataacacaaa gatattagaa gaatatgtta gccaaaataa tcgtattata
      721 gaaatcttga ttgagcaaaa tagaggaagt gaactttgat atggcaaagg ttatagacaa
      781 acacatttac tttcaccaaa cacatatcga tgtattggat gaaactattt ctactcatgc
      841 agaggttaaa aactatagtg aagctgttcg ctttttatgt atgaactatg atccagaagt
      901 ggagaaaaaa gtaaattcca atcaagcaaa gttgaatgga atgagtaaag aaatttctat
      961 tttaaccgaa atggtttcag aatttgcgga tcttcatttg aaagatacag gaatattaat
     1021 tggtaaaaat tcaactgttt atcaagaagc aaaagaacga gtggaaggaa aaattaaatc
     1081 caatcaaaca aaaaaatatt cccctcagtt gaagaaacct gtgaatgaat ctgttcatgc
     1141 cagagtagat ccatttgatc caacgaagat tctaaaaaga taaaaatgaa ggaggataat
     1201 aatgactcaa tcgccttcag ttattctcac ttgtcaattt actttgccga ataaaaagtc
     1261 attttctact tatattgact atattacaag ggaacaggct cttgaagaaa aggaacgacg
     1321 tacgcccgaa gaagaacagg aacttcaacg aatcaagaat gctttagagg agttttatat
     1381 tcctgaagga gaaacttttt cggagagtaa gacgggtaaa gaattcagtg aaacgtcgaa
     1441 tgaagctcaa tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt
     1501 taccaattat ctttcttata tgacacgtca ctatgcgtta gaacaaaaga aagaactgac
     1561 gcaaagtgaa caaaaagaat accaacgctt gaaccaagca atgaaaaaat atcaaacaga
     1621 accccataaa aaagcttcag gaaatcttcc tggagttttt tctatagctt cagacgaagt
     1681 gaaaggaagt gatctgaagg agattaagca aatcttccaa agaggacaac aaaaaggctc
     1741 aatcatttat caagatgttg tttctcatga tcattcgtat cttgaaaaaa taggattgta
     1801 tgatcccaaa acagatactt tagatgaaga agggttaaaa gcagctggaa gaaaaatgat
     1861 ggagacatta ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca
     1921 ccgtaacact aaacatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa
     1981 accttacacg gaggttgaaa atggtgtgag ttatatcgta ccgaaaggta agagaagaca
     2041 agcaacatta gacgcaatga agaccaatta tgtacatgag ttagaacgtt tttcttatga
     2101 aaaggatcga atgattggta tggagaaacg aaatttattg acgcggaaaa gtgatttgcg
     2161 taacacctta acaaaagtgg taaaagagcc agcgcgttac gatcgacacg cgatgaactt
     2221 attaaatgaa gtatataaaa ccctacctga caaacgttcg gaatggaatt acggggatga
     2281 aaaacgaacc aaactttcac catacacaag agagaaactc gatgagttaa ccaaatatgt
     2341 tttaacaaaa gaacctgaat atcaagaata tatacagatt tcaaaagcat tgaaagaaga
     2401 agcaaaaagt ctttatgggg aaagcaagag agaatcaaag gatgcagaga aaaatgctct
     2461 ctttgattta aaaaagcgca caggaaatgc catcctaaca gagctgaaga gacaggatag
     2521 tcaaataaaa ccattaatag aaagtgttga tcagatgcaa tctatttaca atgaacgatc
     2581 ctttcgaacg cttaaacaat tgccaacgaa tgaatacaaa tatagttacg aagaattcat
     2641 gaagcgcagg aaacaattcc aacaaagagt tgtcacgcag cggagtttgc gtcaattaaa
     2701 tcgaacgatt aatgcccatg aagaaaaata ccacgcaagt aaagcatacg aagagcaaca
     2761 acgaagaatt caaatggaac aagaaagaca gtaaagacag taggaggaat agcaatgaaa
     2821 caatatttag atcaatggaa agttatagaa ggatcattaa gagaagaacg aatcgaacaa
     2881 cttcctgatt gtttagaaaa agagcatttg ttccagataa gagaaatgtt gagaaacgaa
     2941 cagtttgatc caaatcaatt tttagtcgtt gagtacccag ctactggtgt gtattgttgt
     3001 aatcatgtga atggtgaaaa atattttatc attcaagaat atgagggcaa gttagcacct
     3061 tattatacta cttgggaaat gaatgaagag ggaatcaata actttccatg taaaagc
//
LOCUS       DANJMA010000123         2930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000123 DANJMA010000000
VERSION     DANJMA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>2930
                     /locus_tag="PWU31_002729"
     CDS             <1..>2930
                     /locus_tag="PWU31_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1085186.1"
                     /translation="QQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASAS
                     LIESEYKKWQATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGAT
                     STEAMQMEAKLNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDK
                     LTELGKQAVDSANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDA
                     LILVKRNLGDLNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDL
                     MVSGTQNGLDKTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLV
                     KEMGISLTDGRFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQL
                     NKASTIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELK
                     DSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVG
                     FMALGGPTTLIIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGA
                     SSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWV
                     SNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAV
                     WTPISDFLKNTWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNS
                     ISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTS
                     SISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAIS
                     SGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIP
                     LPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSV
                     LQEIG"
BASE COUNT          986 a          452 c          663 g          829 t
ORIGIN      
        1 caacaacgtt tgtcaaacga aattgataaa acgagtaatg cactagctca agcaaaaggc
       61 gaaatacaga cgtacgagtc tacaatgcaa cagttggata gtgaacaaaa aaatgttcaa
      121 gctagtgctt ctctgattga atccgaatac aaaaaatggc aagcaactgc tggtcaatca
      181 gcttctgaat ccgagaaatt agcgaaagcc caagaatatg tttctcaaca atctgaaaat
      241 gcagagaaaa cgatagatat cctgagacga cagttagaag ctacacagtc tgagtttggc
      301 gctacatcca cagaagcaat gcagatggag gcgaagctta atgatgctga acgtgaattt
      361 gaagagttag gacaagctgc taaaaatgta gatacaacta acttggacga tatcggaagc
      421 aaaatagata tgaataatct aatggaagct tctgacgttt taagcgacat tggcgataag
      481 cttacagaat tagggaaaca agcagtggac tctgctaata gtgtaggtag ttcccagagt
      541 aaaatacaag ctaattttgg tttgactaaa caagaggctg aagaattaac gaatgtagcc
      601 agagacattt attataaagg ttttggagaa tcgttagatc agtccacaga tgcattgatt
      661 ttggtaaagc gtaatttagg cgatttaaat aatcaagatt tacaaaatat cacggaacaa
      721 gctatggtcc tagaaaacac catgggcgct gatatggatg aaacgttacg tggtgtaaat
      781 ggcttaatgg tcaatttcgg cttgagcgct caagatgcaa tggatttaat ggtttcgggt
      841 actcaaaacg gtttagataa aacgcacgaa ttaggcgaca atatggcaga atatagccaa
      901 ttatggagtc aaatgggata ttcagctgat gaaacgttcg gaatgcttca aaatggttta
      961 gatgcgggtg cttataacct tgataaagtc aatgacttag ttaaggaaat gggaatatcg
     1021 ttaacagatg gtcgatttga gcaaaacatg gatatgttta gtgaaagtac tagaaaagct
     1081 tttgaagagt ggaaaaatgg cggaggaaca caaaaagacg ttattaattc catgattcaa
     1141 gattttagca atatggatgg tcaatacgac caattaaata aagcttctac aatttggtct
     1201 gcacttggcg aagataacgc gatgaaagtt gtccaatctt tgactgatgt taaccataca
     1261 tttgatgatg ttagtggatc tgcacaaaaa atgaatgaag attctactac tccgttacaa
     1321 gaattaaacg gaaaaatagc tgaattaaag gattcattag ctcctatagg caacacaatc
     1381 atagatgcac tcgaaccagt aattgatttt ctaggaaaga tggctgatgc gtttaataat
     1441 cttccacaac cagtacagga ttatgccgta gcgattggcg gattgactgc tgcatttact
     1501 ttattaatgc caataatagt tggcttcatg gctctaggtg gtcctactac attaataata
     1561 ggagcagtta ttactgttat tgctggagtt atagcaatta taaaaaactg gggcgcaatt
     1621 actgactggt ttaagggaat atggagtaaa ttcactgatt ggttgggtgg tacttgggaa
     1681 agtataaaag aaggtgcctc atcagtttgg gatggagtta aagaaacctg gtctggattt
     1741 gtagattggg ttcaagatat ttggcaagga gtttctgatt ggtttggaga gctatggagc
     1801 ggattagttg aaggagcttc caacatctgg caaggagtcc aagagacttg gcaagcattc
     1861 gttgattggg tttcaaatat ttggaacgga gtcaaagaag tatggtcgat tatttgggca
     1921 gacattgtag gaattgttca aataccatgg accttaataa cgtcattgat tcaagccggt
     1981 attaatatta tcgtgggtat ttttgatgta gctggacagt tattaggcgc agcttggcaa
     2041 gctgtttgga cacctatttc tgatttcctt aaaaacactt gggatactat gacacaatgg
     2101 gtaagcatcg cttggaatgg aattgtaact acattccata ctatatttga tccagtagtg
     2161 gcatggtgga atggtatatg gacagctatt agtactacgg cttcaaatat ttggaattca
     2221 attagtgcaa cagcttctag tatttggaac agtatcaaga atacaatcac tagcttggta
     2281 caagcagctg ctacagtaat tcaaaatatt tggtcaactg tatctagttg gttaggtgga
     2341 atttggaatt caatcagctc tacagcatca aatatctgga atagcgtgac tagtagtata
     2401 agcaatgcta taaacgcagc taaaagtgcc attcaaagtg tttggaatag tatatcttcg
     2461 tggatcagcg gaatttggaa cggtatcaaa aacactgctt tgaatctttg gaatggaatt
     2521 acaagcacta ttagctctaa agtaaacgat ggaaaaaatg caatttcaag cggttggtcc
     2581 aatctaacag gtattgtttc cgacatattc aataatgtta aaagtacaat tgctaacata
     2641 tgggaaggca tcaaaaagac tgttagcgct ccaattgatt ggattagaga caaaatcagt
     2701 ggcatttttg ataatttgaa tatttctata ccacatattc cgttaccgga atttatcatg
     2761 gaaggcagct ttaacccgct aaaaggtcaa atcccccatt tgcgtgtaaa atggcatgct
     2821 aaaggaggta tctttactaa accaacttta ctaggtggaa tgaacggtgt cggtgaagca
     2881 ggacctgaag cagttttacc tttgaaaaga tctgttttgc aagaaattgg
//
LOCUS       DANJMA010000124         2914 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000124 DANJMA010000000
VERSION     DANJMA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            137..748
                     /locus_tag="PWU31_002730"
     CDS             137..748
                     /locus_tag="PWU31_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1085187.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="PWU31_002731"
     CDS             821..1750
                     /locus_tag="PWU31_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL1085188.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="PWU31_002732"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="PWU31_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..>2914)
                     /locus_tag="PWU31_002733"
     CDS             complement(2693..>2914)
                     /locus_tag="PWU31_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085189.1"
                     /translation="PLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSI
                     FDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1001 a          407 c          544 g          962 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atgg
//
LOCUS       DANJMA010000125         2839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000125 DANJMA010000000
VERSION     DANJMA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..1398
                     /locus_tag="PWU31_002734"
     CDS             <1..1398
                     /locus_tag="PWU31_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1085190.1"
                     /translation="GEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGN
                     YEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLP
                     ALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGS
                     SDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEK
                     VDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEP
                     NAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSS
                     NKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
     gene            1532..>2839
                     /locus_tag="PWU31_002735"
     CDS             1532..>2839
                     /locus_tag="PWU31_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1085191.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1110 a          388 c          557 g          784 t
ORIGIN      
        1 ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac taacttgcca
       61 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      121 acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      181 gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata tgaaccaaca
      241 gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa tgttactaac
      301 ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat
      361 accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa
      421 gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga taaatacaat
      481 cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt
      541 aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa agtagatgtt
      601 agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg atcatctgat
      661 gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa atataatcct
      721 gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga gggaattaag
      781 aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt agatgttagt
      841 acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc taaagaagaa
      901 gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca agttgatcct
      961 attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt
     1021 acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa ttttacagtt
     1081 aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga tgaatctggt
     1141 aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac
     1201 ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga
     1261 ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag taatatcttt
     1321 actattagcg gtggtttaat actgttagga acgttaggat tattagggta taaaaatcga
     1381 aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga ggaagaatct
     1441 ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa gatgaatata
     1501 ctttttatag aaaggaaaag aatgtaatta agtggaaaaa atagtaatta ttggttcaaa
     1561 ccattcaggg ctagccgcag ctcgatttct aaacttaagc gattcaaatt atgaagttat
     1621 tctaatcgat aaaaaatcag aaattagtta cttggaatgt gggacatcgt tatttataaa
     1681 aggagaagta agtaatttag aagatctttt ttatcaaaat agaatgacac taaattctga
     1741 gataaaaaag ttatttctag agacagaagt tgacaaaatt gatattcaaa ataaaaagat
     1801 tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc
     1861 tacaggttca aagcaaatta tgctagatat accggggaat aatttagcta atattttttc
     1921 gataaagaat tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa
     1981 aaaagtagga attattggag caggatatat tggagttgaa attgctgaag ccataagaaa
     2041 aagagggaaa gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga
     2101 ccgtagtttt tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct
     2161 atcagaaagt cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag
     2221 tggtacccaa tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc
     2281 aattggggaa aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca
     2341 gcaaacaaat ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc
     2401 cattaataga agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc
     2461 aactcaacat attaacaatc aagattttac accttctggt tcccaattta caaatggctt
     2521 ttctatattt aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt
     2581 aagtgagtct atagattata tagaaataga agatttcatt agacctgctt ttttgagaaa
     2641 aaatagtcgt gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt
     2701 acaactttgt agtcaagaag atttatctgg actattatca atgttttctc ttgctataga
     2761 agaacaaatt acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag
     2821 ccaaccctat aattttatt
//
LOCUS       DANJMA010000126         2819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000126 DANJMA010000000
VERSION     DANJMA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>2819
                     /locus_tag="PWU31_002736"
     CDS             <1..>2819
                     /locus_tag="PWU31_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1085192.1"
                     /translation="QQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSAS
                     LVESEYKKWQATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGET
                     STEALEMKTKLNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDK
                     LIDVGSKSIEAAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQY
                     TSMFKGLGYDTKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIG
                     LFANDTQMAAYAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEARE
                     AALKLKDAQDKINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDG
                     LENQMTRAKQALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILA
                     LGGILALLSTLAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAIT
                     DWFSDLWKKFTDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELW
                     SGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLI
                     QAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTI
                     FDPVVAWWNGIWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWST
                     VSSWLGGIWNSISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKN
                     TALNLWNGITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVS
                     APIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNS
                     PNIIGVGEAGPEAVLPLKRSVLQEIG"
BASE COUNT          956 a          421 c          653 g          789 t
ORIGIN      
        1 cagcaaaggt tatctaatga tatagataaa acgagtaagg cattggctca atctaaaggt
       61 gaatttaaaa cgtatgagtc aactatgaaa gacttagata atgagcaaca acatctaaag
      121 tcaagcgcat cattagtaga atccgaatat aaaaaatggc aagcaactgc tggccagtct
      181 gctacagaat ctgaaaaatt agcaaaagca caagaatatg tcggcaaaca gagtgatatt
      241 gctgaacaaa aaatcgaagt gctaaaaaaa caattagatg ccacacaaaa agaatttgga
      301 gaaacatcta ccgaagcttt agaaatgaaa actaagctta atgatgcgga aagagaattc
      361 gaagaattaa gtaatgctgc taaaaacgtt gatacctcca cagtagatga tatcggcaaa
      421 aaacttgata tgggtaattt aatggaagct actgatcact tgtcagtgat cggagataag
      481 cttattgatg taggtagtaa gtctattgaa gcagctggaa aagcacaagc tatgcaggcc
      541 caatttaaac aagtctttgg ctctttagaa ggggaagcac aggacgccgt tgagggaatg
      601 gctgaagaat ttggaatgtt accaaatacg atcaagcctg tttttacaca atatacgtca
      661 atgtttaaag gacttggata tgataccaaa gaagctatgg agttagctgg tgatagtact
      721 cagttagcag cagatgcagc agctttttac gataagtcta tggatgatgc tagtgaatct
      781 cttaattcat ttataaaagg gaactacgaa ggtggggagc aaataggttt atttgctaat
      841 gatactcaaa tggcagctta tgctgttaag cataatttga taccagcgac tgaaggagca
      901 aaaaaagcca gtgaagagtc attgttagct gttgaaaaag cacaatctaa gtatgctgat
      961 gctattaaga aacatggtga aggatcttta gaagcaagag aggctgcttt aaaacttaaa
     1021 gatgcgcaag ataaaataaa tgaagaatta ggcccacaaa cgcaaaaatg gtctgattta
     1081 gatgaggcta ccaagcaagc agttcgagtt caatatgctg aagatatgca aaaattagcc
     1141 ggtgctacag gtcaggctag tagggagtct gatggtttag aaaaccaaat gacaagagca
     1201 aagcaagcgt tagaagattt ttatgcctca ttgggtgaag atatactgcc tgtgtttatc
     1261 aaagggttgc aagcaggagc gaaagctttg caaggattag ccgaatggtg gagtaaactt
     1321 gatgggccaa tgaaaaattt cattttagct cttggaggaa ttctagcatt attaagcaca
     1381 ttagctcctg ttataaccgc agttgttacg atagttggca catttggttc tacagttttg
     1441 ctgccaataa taggaattat agcgggcgtt gcagctgtaa tagcgattgt aattacagcg
     1501 tttcaaaact ggggcgcaat cactgactgg tttagtgatt tatggaaaaa atttaccgat
     1561 tggttaggtg atacctggga aagtataaaa gaaagtgcct catcagtttg ggatggagtc
     1621 aaagaaacct ggtctggatt tgtagattgg gttcaagaaa tttggcaagg agtttctgat
     1681 tggtttggag agttatggag cggattagtt gaaggagctt ccaacatctg gcaaggagtc
     1741 caagagactt ggcaagcatt cgttgattgg gtttcaaata tttggaacgg agtcaaagaa
     1801 gtatggtcga ttatttgggc agacattgta ggaattgttc aaataccatg gacattaata
     1861 acgtcattga ttcaagctgg tattaatatt atcgtgggta tttttgatgt agctggacag
     1921 ttattaggcg cagcttggca agctgtttgg acacctattt ctgatttcct taaaaatact
     1981 tgggatacta tgacacaatg gataagcatc gcttggaatg gaattgtaac tacattccat
     2041 actatatttg atccagtagt ggcatggtgg aatggtatat ggacatctat tagtactacg
     2101 gcttcaaata tttggaactc aattagtgca acagcttcta gtatttggaa tagtatcaag
     2161 aatacaatca ctagcttggt acaagcagct gctacagtaa ttcaaaatat ttggtcaact
     2221 gtatctagtt ggttaggtgg aatttggaat tcaatcagct ctacagcatc aaatatctgg
     2281 agtagcgtga ctagcagtat aagcaatgct ataaacgcag ctaaaagtgc cattcaaagt
     2341 gtttggaata gtatatcttc gtggatcagc ggaatttgga acggtatcaa aaacactgct
     2401 ttgaatcttt ggaatggaat tacaagcact attagctcta aagtaaacga tggaaaaaat
     2461 gcaatttcaa gcggttggtc caatctaaca ggtattgttt ccgacatatt caataatgtt
     2521 aaaagcacga tagctaatat atgggaaggt atcaaaaaga ctgttagcgc tccgattgat
     2581 tggattagag ataaaatcag tagtgtcttt gataatttga atatttcgat accacatatt
     2641 ccgttaccac attttaaatt gagcggagaa ttcaatccat tgaaggggaa aatcccaaca
     2701 ttgggtgttg attggtatgc gaaaggtagt gtgtttaatt ctccaaatat tatcggtgtc
     2761 ggtgaagcag gacctgaagc agttttacct ttgaaaagat ctgtgctgca agaaattgg
//
LOCUS       DANJMA010000127         2793 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000127 DANJMA010000000
VERSION     DANJMA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            318..614
                     /locus_tag="PWU31_002737"
     CDS             318..614
                     /locus_tag="PWU31_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085193.1"
                     /translation="MKKFKKKIYLGLAVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVYVHHSLLLILFLLLSLS"
     gene            complement(645..893)
                     /locus_tag="PWU31_002738"
     CDS             complement(645..893)
                     /locus_tag="PWU31_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085194.1"
                     /translation="MAEAKKSYADMTEEEAQYAMVLGARIAHAREGLGISQGELGKLA
                     GFNQTTISLIETGKRLPNLKTLIRLSKILKKSLKITLE"
     gene            complement(955..1191)
                     /locus_tag="PWU31_002739"
     CDS             complement(955..1191)
                     /locus_tag="PWU31_002739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085195.1"
                     /translation="MEKQKQVITRKQYEEIMARYDKVDYAVIAGFIIFGTVIFGFGGF
                     VFMAIVGAFTFPRMKQKSINLIEQDYIVRDDIQQ"
     gene            complement(1400..1789)
                     /locus_tag="PWU31_002740"
     CDS             complement(1400..1789)
                     /locus_tag="PWU31_002740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085196.1"
                     /translation="MDVGHTETITKTFNIEPLTVYSSPENIYAKIGNTKDSYVNFAIT
                     GKGTYSAPLTKANIHYVESEGRPKVTVTYYKLDSPLSAFIGIQGEIADSKTRIDIQVP
                     IGGVDVTNTGSTFEEQSVNDLLKQFPK"
     gene            1969..2434
                     /locus_tag="PWU31_002741"
                     /pseudo
     CDS             1969..2434
                     /locus_tag="PWU31_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          917 a          456 c          500 g          920 t
ORIGIN      
        1 acaagatacc gaagactcac tgcgcaaagg gtggggaatg aataataaag ttagataata
       61 atcaaagaag aactaagtaa ctacctttaa acggtagtta cttagttctt ctttgatttg
      121 cgtgaaatag aggggattta tttttctggt tacgtgatta aaaaatccaa aaaatgagca
      181 taggaacggt tatgatttta gggtgtgttt taatgagata ttgtgatttc attaacaagt
      241 gtatgataat ctgttgttga gttagtaaat aagtaataga gctaactcta aaataaaact
      301 attaggagga gatgtagatg aaaaagttta aaaagaaaat atatttagga ttggctgttg
      361 gggtgagtat gtttgggttc atggctcata ctaaacaagt aactgcagat actaaccaaa
      421 aagaaaggat tatgagccaa ttagatgact ctcaaataga gtatgtaccc aatgagctga
      481 ttgtcagttt aaaggctaca caaagaattt caacagagaa aaataaaccg caaaatttat
      541 tgggtgtaaa agttatggtg tacgttcacc attctttact tttgattttg tttcttttac
      601 tttcgttgtc ataataaatc tccccctgtt ttattttctt tacctcattc caatgtaatt
      661 ttcaaacttt ttttaagaat cttactcaat ctgataagag tctttaagtt aggtagccgt
      721 ttacctgtct caattaaaga gatagttgtt tgattgaaac ctgctaactt acctagttct
      781 ccttgactaa tacctaaccc ttctcgtgca tgagcgattc tagcacccag taccattgca
      841 tattgtgctt cctcctctgt catgtcagca tagctctttt ttgcttctgc catttctttg
      901 tcctccttgt accattatag taacaccttt aatttccata gtcagcaatt aagcttactg
      961 ttggatgtca tctctaacaa tgtagtcttg ttctattaaa ttaatgcttt tttgtttcat
     1021 acgggggaac gtgaacgcac caacaatagc cataaaaaca aatcccccga aaccaaagat
     1081 aactgtacca aagataatga accctgcgat gacagcgtag tctaccttgt cataacgtgc
     1141 cataatttcc tcgtattgtt ttcttgtaat cacttgtttt tgtttttcca ttctaatttc
     1201 ttcctttgtt ttatcttaca agtctattgc acagcctgta ttgtttaatg tcaataggta
     1261 agctcaaatt ttttatttat tcttgctttc cttatctaca tgtatatcat agcctgattg
     1321 tatagggatg tcaacaaaaa aagagacacc tataaaagat gtctctagcc tgctacttag
     1381 ggttttttat tgtagcccgc tacttaggga attgtttgag taagtcgttt actgattgct
     1441 cttcaaaggt gctacctgta tttgtaacat cgactccgcc tataggaact tgaatgtcta
     1501 tacgagtttt actatctgca atctcccctt gtattcctat aaatgctgag agaggagagt
     1561 ctagtttgta ataagtaacc gtgacttttg gtctcccttc tgattcaaca taatggatat
     1621 tagcttttgt tagcggagca gagtacgttc ctttacctgt aattgcaaaa ttaacgtagc
     1681 tatctttagt gttgcctatt tttgcataaa tgttttccgg tgagctatag acagtaagtg
     1741 gctcaatgtt aaaggtttta gtaattgtct ctgtatgccc aacatccaat gtacccacgc
     1801 ctatcagtac ccaaatagca aaaacaacta aatctataac aagaggcttc cagtaacttt
     1861 tacgaagata ccaacgtgga ttttttctat tggtgattga tgagagttac cgtttgtaac
     1921 tgaatatcgt agataagtca ttgacgatga aagatctaaa tttgctaaac ttctaagatg
     1981 gagaagaaac taaaacgtga acaacgtaag ttatcaagac gtgcgttgtt agctaaaaag
     2041 aaaggaatca atctatttga agcgaagaat tatcaaaagc aaaaacaaaa agtagctaag
     2101 ttgtacgaaa aagtaatgaa tcaacgtact gattttctga ataagttgag tacagaaata
     2161 atcaaaaacc acgatattat ctgtatagaa gacttaaaca cacaaggtat gttgcgtaat
     2221 tataaattgt taaaaaaagt atttctgatg tatcttggtc tagctttatg gttaatttac
     2281 aatataaagc agactggtat ggacgagaaa ttatcaaaat aggtcaattt ttccatccag
     2341 tcaaatctgt tcagaatgtg gatataaagg cagcaagaaa tcactcaaaa ttcgagagtg
     2401 gatttgctct atttgccata cacatcatga ttgagacgtt aacgctagca tcaatattct
     2461 gaacgagggt ctacgaatac gagcgttaat ttagaaaaat acatttaaga accgtaggaa
     2521 ctacggggat agcttggtaa ataagagtca ttgctgttag gaaataaatg cactatcaag
     2581 taagctcttt tcccaaaaag cttttgcttc aagtgttaag aaacatcagg tttagctaag
     2641 tgatgagcag ttcacaagta aataaacaat gtatcattgc acatttgttc cgtttttgaa
     2701 gtgtgaaatc aaaataacat gaagcatatc gaaatttcgg gagagtgtaa aatattttgt
     2761 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DANJMA010000128         2633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000128 DANJMA010000000
VERSION     DANJMA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..195)
                     /locus_tag="PWU31_002742"
     CDS             complement(<1..195)
                     /locus_tag="PWU31_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL1085197.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(211..453)
                     /locus_tag="PWU31_002743"
     CDS             complement(211..453)
                     /locus_tag="PWU31_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDL1085198.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            complement(531..>2633)
                     /locus_tag="PWU31_002744"
     CDS             complement(531..>2633)
                     /locus_tag="PWU31_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085199.1"
                     /translation="GDKGITVTVKPFLNGLQMDTSGGTFTLKGTTPSNRYVDSVATSV
                     TSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQILTTQDIIFFSLGVSDISQGQADEYV
                     SQLEELIRKYNETFDAFMAEIKGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGN
                     LQVMYSNSIDFGGYDYSGNPNLMANINADSFSQGTGALSVVDDGDEVVVTLDPNHKLE
                     LFKAKSQPNLLIGKTYTMSVEIMLEDDFTGDPSKIGLRYTKMPNWVSELYTRNTLTTA
                     KGVWQKLTGTVKITATSDNAESWFIMLQNKDANNSLSGKLRLRHAKVEYGSTATPYQP
                     NLFDAPYYLSKAALGENIANKSVTFPIKSSDYAVYDYKNIENFVVGEKYVATIKATKP
                     ATQVFEVRNTNSYGFGAMKPVEGLTDIWVLEFTPTRIDAASPSSLQIYQSPKNTKGAV
                     QIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAPEYVENNLATDIKVAEI
                     TGKANNYTDGKVSQINSQLTASINAVDTTAKDAQTKANANATAIDERINDTATTAFYG
                     AQGEVSNKTKVAEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGG
                     KYASWLYTDLYKDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVAD
                     GILQFTSCRTLVLTRLSD"
BASE COUNT          730 a          568 c          418 g          917 t
ORIGIN      
        1 ttttttcaaa ttataagccg acacaccagt gataacacct aaaaacgtgg ctactgcatt
       61 gatagtcaga actgtcatat ctgttccatt ccatccatag gctttgccta acatggctac
      121 taaagcagat gcagctggta atactgttaa aaccgtccat ttaatgactt gataatactt
      181 atctggtaaa atcatttctt ctcacctcct ttacaattta gtcaagaaat agccaatgat
      241 tgtaatgcct aagccaatca tatagcccca agcccattta ttattgttct tcatttcctt
      301 gatgtcttct gcattattaa gcgcaataga atatacctga tccgctctat cttttgcact
      361 ttccgctttt tcacgtaata attcgtaatt atccagtttc gtttcaatac gcactaagcg
      421 ttctaccacg tcttgtattg cttcgtcttt caaccaacta gcctcctttc attgcaaaat
      481 aaaaaacacc ctcctttgag cgtgttgata gcaaaattat aaatattgta ttaatcagat
      541 agtctagtca agactaaagt acggcaagat gtaaattgta aaattccatc tgcaacgccg
      601 atttcagtac gcatatttag tacatcgttt acttctaaat cagttacaat gtttccgcta
      661 gctgcaaatc tattttgcaa ggcgtcgacc ccataagata ctaaattatc aacagaagtt
      721 ccatctttat ataaatcagt atataaccat gaagcatatt tcccgcccaa ttgcacacgt
      781 atttgcgcac taattgaata cttaccagct tttaagcatt taacgtatcc gttagattga
      841 cgttcgaaat gtgtttctcc tacagctgta gtagtatacc ctgttcctac tccatactcc
      901 gctactttcg ttttgttaga gacttcacct tgtgcgccat aaaatgcggt agtagctgta
      961 tcattaatgc gttcatctat agcagtcgca ttagcattcg cttttgtttg agcatcctta
     1021 gctgtggtgt ctaccgcatt aatcgaagca gtcaactgcg aattaatctg cgatactttc
     1081 ccatcggtat aattgtttgc tttaccagta atttcagcaa ctttgatatc tgtagccaga
     1141 ttgttttcta catattctgg tgctaaatcc cagacatagt cttttggatt atttgagtct
     1201 cgcatgccga ttcctcggta tttatattgc tcaatattcg gggttcgtgt gtcgcctttt
     1261 tcgatcttga gccagtcgat ttgaacagct cctttagtat tctttggtga ctgatatatc
     1321 tgaagacttg atggactagc agcatcaatt cttgttggag taaactctaa tacccatata
     1381 tcagttaaac cttctactgg tttcatagca ccaaaaccat aactatttgt atttcttact
     1441 tcaaatactt gtgttgcagg ttttgttgct tttatagtcg caacatattt ttcccccact
     1501 acaaaatttt caatgttttt gtagtcgtac acggcataat cactagattt aattggaaac
     1561 gtcacagact tattagcaat attctcaccc aaagccgcct tactcaaata atacggtgca
     1621 tcgaataaat ttggttgata tggtgtggct gttgagccgt attcaacttt agcgtgtctc
     1681 aaacgtagtt tgccagatag gctgttatta gcgtctttat tttgtagcat tataaaccag
     1741 ctttcagcgt tatcactcgt agcagtaatt ttaacggtgc cagttagttt ttgccataca
     1801 cccttagcag tagttaatgt attacgcgta tatagttctg atacccagtt aggcatttta
     1861 gtatatctta gacctatctt actagggtct ccagtgaaat catcttctaa cataatttct
     1921 acactcatgg tatatgtttt acctattaac aggtttggtt gacttttcgc tttgaataac
     1981 tctaatttat ggtttggatc aagcgtgacc actacctcgt caccatcatc cacaacagat
     2041 aaagcaccag taccttgcga gaagctgtca gcgtttatat tagccattaa attcggattc
     2101 ccgctataat catagccccc gaagtcgatg ctgttactgt acatgacttg caagttacct
     2161 aatttagaaa tttcttcttt cagagcatct aacttgtctt gtagcgtttt agcttgacca
     2221 gttaaatcag taatctgttg atttaagcta tccactctac ctttaatttc agccataaaa
     2281 gcatcaaaag tttcgttata ctttcgaatc aactcttcca attgcgaaac gtattcatcg
     2341 gcttggcctt gcgaaatgtc agacactcct agtgagaaaa aaatgatgtc ttgcgttgtt
     2401 aaaatttgat taccttttct atattctacg tagcagtgtt tataatatcc tgcttcactc
     2461 ataaatgtgc catcaagaga aaatatgact tcttcactag ttacgctagt tgcaacacta
     2521 tctacataac ggttagatgg tgttgttcct tttaaagtaa atgttccgcc actcgtatcc
     2581 atctgcaagc catttaaaaa tggtttaaca gtcaccgtaa tccctttatc acc
//
LOCUS       DANJMA010000129         2605 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000129 DANJMA010000000
VERSION     DANJMA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(313..903)
                     /locus_tag="PWU31_002745"
     CDS             complement(313..903)
                     /locus_tag="PWU31_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL1085200.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1426..1587
                     /locus_tag="PWU31_002746"
     CDS             1426..1587
                     /locus_tag="PWU31_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1085201.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1606..2100
                     /locus_tag="PWU31_002747"
     CDS             1606..2100
                     /locus_tag="PWU31_002747"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1085202.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2093..2254
                     /locus_tag="PWU31_002748"
     CDS             2093..2254
                     /locus_tag="PWU31_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085203.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          921 a          409 c          447 g          828 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagt
//
LOCUS       DANJMA010000130         2580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000130 DANJMA010000000
VERSION     DANJMA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..280)
                     /locus_tag="PWU31_002749"
     CDS             complement(<1..280)
                     /locus_tag="PWU31_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1085204.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVSH
                     ATIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAI"
     gene            complement(354..635)
                     /locus_tag="PWU31_002750"
                     /pseudo
     CDS             complement(354..635)
                     /locus_tag="PWU31_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            1097..2478
                     /locus_tag="PWU31_002751"
                     /pseudo
     CDS             1097..2478
                     /locus_tag="PWU31_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          925 a          447 c          427 g          781 t
ORIGIN      
        1 cgatggctcg atacaaataa tgccattttc ctttaatttt gatgtacgtt tcatccattt
       61 tccatgaata aaaggatttt ttatttttct ttttccaaat ttgatagagt agtttgccat
      121 attcttgcac ccaacgataa atcgtcgcat gagaaacgtt aatgccacga tcatataaca
      181 attcctgaac ttcacgatag cttagattgt aacgcaggta gtaaccaaca gcgacaataa
      241 tgacgtcttt tttgaattgt ttgcctttaa aatgattcat tactctgccc tctctgtctt
      301 ttttctcaat tttacactaa aatagatttt ttggaaaact ttgcaacaga accgaaatga
      361 gtatagtatg aattagtaat ttggttttca tctaagttta gctttgattt actaaaattg
      421 atatctcgat acatgttcaa tacatctaat acaaatcgag aatcttcttt cggaaatggc
      481 tcagaaagat cactccaaat ttcatcatca taatgatact cgtaaccttc tctcaatatt
      541 tcgatttttc tttcataata agatctgttt tggtcctcaa atttagccaa taactcaaat
      601 tgatttacta gtgacaatct ctgaaaactg tccattaaat gtttcttcct atcttgttca
      661 ttaacaaaaa attataagcc cttacaacga atattatata ttaaatttaa ttgtaggtga
      721 acacgaattt tataaatatt tatggtgtta atgacaaatg atatccgact cctcttcata
      781 cgttctggac tatttgctaa tagattaaaa tttcagcatc tatttctgac cataaataaa
      841 tgagctgccg aaaagctgcc aaatcagact tgaaaataaa ggatcaaaaa cttttaagtt
      901 tctgaccccg taactcacac ttcaaaatct tacttaaaaa ggagtctgaa agaaaaaaag
      961 agtattcaaa atttttttct aactagctac atcacatcta tgcattgaca ctcaaagaac
     1021 cagaataagt catcagaaag caagggggtg cggacataat cctggacgaa aaaataaact
     1081 taggaggaat gtccgtgtgt tttcccacaa agagcgtgta agggcaattc agttattttt
     1141 aaaatacgat tgctcttatg ctgcaaccat tcgagaatta gggtatccgt cgattggtgc
     1201 actacggaaa tggtataaag agtatttgga atccggagag ctccatcagg agcaccgaaa
     1261 aaaatcaaag tattctgaag aacaaaaacg aatagcggtg aatcattatt ttgaatatgg
     1321 tcaatgttat gcacgaacca ttcgcatgct tggttatcct aatagagagt ccttgcggca
     1381 gcggtgtgaa gaacttgcac caggagctag aaaactccga aaaagtgcgg taaagttaac
     1441 acaagatcaa aaagaagctg tgttgaaaaa attctataaa ccgcaaacta ataggaaaaa
     1501 tttagcagaa tctgaaggaa ttagtagagt tactctatat caatggaaaa acacggttct
     1561 tggaaaggat tttccattgc gtatgacttt aaaaaatcag gaaaaacaaa aagagctact
     1621 tttaaaggaa gtagaagact tacaaaaaca agtacaccaa ttacagctcg aaaaaacatt
     1681 actcgaagga gcggctgaac tgctaaaaaa agaaaaaggc gtcaatcttc tttgtctatc
     1741 aaatcaagaa aaaacgatat tgattgatgc cttacgaaat cagttcacct tgaaagagct
     1801 tcttcaacag ctccagttgc ctaaaagcag ctatttttat caaaaacaag cacttgagaa
     1861 accagataag tattacaaag aacgtcaatt aattatcacg attttcaatc ataacttctg
     1921 tgcgtatggt taccgtagaa ttcaccaagc tttaaaaaat atgggtaaaa agctttctga
     1981 aaaagttgta cgtagattga tgactgagga aaatctattt gttaaatttt ctagacgaaa
     2041 aaagtattct tcgtatgctg gggaaatttc tcctgcacac cccaatttat taaatcggaa
     2101 ttttaaagca aagaagccaa atgaaaaatg gttaacagat attactgaat ttagaattcc
     2161 cgcagggaaa atctacttat caccacttgt cgattgttac gatggtgcta ttgttagttg
     2221 gactattgga acaaagccaa cttgcttcac tctatgtcta aaaagggtta ttcaccagat
     2281 aattcagcgt gcgaaggttt ctttggacga ttaaaaaacg agatgtttta tcaacgtgac
     2341 tggaagaata caacaatcaa tcagtttata aacaaattgg atgattatat tcattggtat
     2401 aataatcaac gaattaagtt atctttagga gggctaagcc ctcttcaata tcggaagaag
     2461 caaagatata tttgttaaaa aggtccaaaa aaatatccgc accccctttt tttgtaaaaa
     2521 actattgcac aatacaagtg gcctacggct atcgaaattt ccataacttt cgagcaagaa
//
LOCUS       DANJMA010000131         2486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000131 DANJMA010000000
VERSION     DANJMA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(1016..1306)
                     /locus_tag="PWU31_002752"
     CDS             complement(1016..1306)
                     /locus_tag="PWU31_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085205.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2206)
                     /locus_tag="PWU31_002753"
     CDS             complement(1514..2206)
                     /locus_tag="PWU31_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1085206.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          735 a          459 c          349 g          943 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt tctacatttg
     1681 ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta ttttgtaatt
     1741 ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc agatgattga
     1801 tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt tgtttgcttt
     1861 gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct gctgccgatt
     1921 gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca tctgcctttg
     1981 aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta atcgcttgct
     2041 ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta tcaatatctg
     2101 tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag ccaattagac
     2161 atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc atagctcctt
     2221 aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca agcgttttta
     2281 aattacatat gtaattttaa tataaatact atcaaatact catttaatcc tcctttttta
     2341 tctatttaaa aaatcttttt tatagagaaa aatgcaggtt aatactttct taaataaata
     2401 tttatggtga acaaaaacaa aaaagcaagt tttattaaac gtaacccttt tcatttcata
     2461 gattactgta tttattacaa aaaacg
//
LOCUS       DANJMA010000132         2406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000132 DANJMA010000000
VERSION     DANJMA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            80..>2406
                     /gene="esp"
                     /locus_tag="PWU31_002754"
     CDS             80..>2406
                     /gene="esp"
                     /locus_tag="PWU31_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL1085207.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          871 a          377 c          488 g          670 t
ORIGIN      
        1 ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt
       61 aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac
      121 aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt
      181 gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc
      241 tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc
      301 ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca
      361 atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt
      421 ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga
      481 tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc
      541 ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa
      601 ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca
      661 aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat
      721 aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca
      781 agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga
      841 tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc
      901 agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg
      961 tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc
     1021 atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc
     1081 atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc
     1141 gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa
     1201 ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc
     1261 aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat
     1321 cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt
     1381 catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac
     1441 aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag
     1501 cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac
     1561 aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact
     1621 tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg
     1681 ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat
     1741 cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg
     1801 ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt
     1861 taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta
     1921 ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac
     1981 tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca
     2041 agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca
     2101 aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat
     2161 ttcagatgtc tataataagt acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt
     2221 tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat
     2281 taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa
     2341 agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
     2401 cgcagg
//
LOCUS       DANJMA010000133         2352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000133 DANJMA010000000
VERSION     DANJMA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..544
                     /locus_tag="PWU31_002755"
     CDS             <1..544
                     /locus_tag="PWU31_002755"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1085208.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="PWU31_002756"
     CDS             complement(1017..1589)
                     /locus_tag="PWU31_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085209.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="PWU31_002757"
     CDS             complement(1605..2210)
                     /locus_tag="PWU31_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL1085210.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          784 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gt
//
LOCUS       DANJMA010000134         2335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000134 DANJMA010000000
VERSION     DANJMA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            119..448
                     /locus_tag="PWU31_002758"
     CDS             119..448
                     /locus_tag="PWU31_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1085211.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            438..725
                     /locus_tag="PWU31_002759"
     CDS             438..725
                     /locus_tag="PWU31_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1085212.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1009..1866
                     /locus_tag="PWU31_002760"
     CDS             1009..1866
                     /locus_tag="PWU31_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1085213.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1867..>2335
                     /locus_tag="PWU31_002761"
     CDS             1867..>2335
                     /locus_tag="PWU31_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085214.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEI"
BASE COUNT          875 a          312 c          463 g          685 t
ORIGIN      
        1 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
       61 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      121 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      181 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      241 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      301 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      361 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      421 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      481 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      541 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      601 gtgggttcaa atatgcgggc acaacatata gagtagctta cgagattata gaaaacaagg
      661 ttattccagt attgttatgt ggaacgcatg aaaactttta tgaacagttg aaaaaattaa
      721 tctagtgatt tgttgtcgtt cttaagaagg acggcttctt tttatcgaaa atctgattcg
      781 tctacatgac taaagtcgcg agtattctct gcttctcgat aaattaaata acgtcattac
      841 tttgaggaaa gctataaatc aatatatttc agcttttcct tatttatgta ctacctttta
      901 cttattaatt ataaggtagt acatatatat ctttttttgg tagtaactaa aaataaatat
      961 gttataatag tatcaaatga atattgctaa aagaaaagag gtaggaacgt ggtagcgaag
     1021 aaatatgtaa tcggtaattt taaaggtggc gtaggtaaaa gtacgtgcgc gcaaatgtta
     1081 gggtttgaat cagcagttaa tgaaggaaaa agaaccttga taattgactt ggatatgcaa
     1141 ggaaatactt ctgatgtgat gaacttaacg catatgaact tcactaacga agaaggaggc
     1201 ggagaaggtg aaccgattgt ttacgaaaat actgtgactg acgtgttgat aaacaatctt
     1261 ccacctaaag aggctgtcta taaagtaatc aataacctct atatacttcc agcagacatg
     1321 tcctttgaaa tgtatgatga ttggatcaaa gaccaatata atgattcatt atcccaattc
     1381 aaatatatga aagaaaagct agaaccatta tttgatgagt ttgacgtcat ctatttagat
     1441 gtcccacctt ctatttctgt atatagtaaa tccgcgatgt acattgcgga ctgggcaatc
     1501 gtcgtcttac agacccaagt aaaatcgatg agaaatgcga tgcaatatct tgagtatatg
     1561 gacttttttg tgaaagaatt tgatacaaat ttatatgttg ctggtgttat tcctttcatg
     1621 ttggaaagcg gtgacgcagt agatcaggaa atgtataaac aagcccaaga gatttatggg
     1681 gaacatttga ttaaaaatgt tgtactcaaa aatgcacgct taaaacgata cgacggatca
     1741 ggtattacga tggagaagac aaagaaaggt ctcttaaaac aatgggatcg caaaagccac
     1801 gaattattta tcagtatttt gaaagaattg gaagaacacg agaattggta tgcggaggtg
     1861 gagtaattgg ctgacatcaa aggacttgat atttttggga acaacaaagg actcgccaaa
     1921 ataaaaaaag aagcagaagc aaagaaaaag cagcaagcaa ttacaccatc tgaaaataag
     1981 caattagaag tgcaccaagt tcaagaaata aaaacttcct ccaatgttga aagacagagt
     2041 agaggaagaa caaaaaaagt tggcgcacca atcagaaagt ttgataaggt ttattccacc
     2101 aagcatcctt taaaactgtc gactttgtta aatgcgactt ccagagtttt agtagaaaag
     2161 tatgagacta atatgacaag agatgaattg cttagaaaag ctttagatga gtatattaaa
     2221 cagaatctta cgcaagaaga taagcaagat ttgtttaatg acgtggtgaa agaactagac
     2281 ttgttcagag aaaaatatcc aattgttcca gaattagatg atcgtggaga aataa
//
LOCUS       DANJMA010000135         2291 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000135 DANJMA010000000
VERSION     DANJMA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            261..1559
                     /locus_tag="PWU31_002762"
     CDS             261..1559
                     /locus_tag="PWU31_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL1085215.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTNKEIEAAKKVADQLKKDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDQFINVKEVYDQKDRG"
     gene            2207..>2291
                     /locus_tag="PWU31_002763"
     CDS             2207..>2291
                     /locus_tag="PWU31_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1085216.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSY"
BASE COUNT          824 a          396 c          411 g          660 t
ORIGIN      
        1 gggtttattt tagtttttct tggaaataaa aacagcgaaa atccgaacat tttcacctct
       61 ctcactttat aacttttaca aaatagtact tctcataaaa agcgctctag ttaaagaaaa
      121 taagagaaaa atcaaaaaaa aacaaaagtt tcttttacac ttgtttctat attcgttaca
      181 atggttatgg gaagtttttc aaaaggttaa gaaaagctta cggaggtttt tgaaatcaaa
      241 aagtagtagg ggacaataaa atgaaacata cacacaaaat catattggct gtcacttctg
      301 tatgcactgt tattattcta ggtgtaagta tcttttttgt tacacaagca gcaacaaaca
      361 ataaattaga aaacactagt caaacaactc aacactcaag cagcagctca aaacctgttg
      421 aagataaaca aacgacgaaa cagcttgatc aagcaaaaca gcttgctgca agttatcatt
      481 atgatgaagc catcgcctta cttgaaaaag acgacgccaa agaagcgcaa caattgttag
      541 caactttgaa aaaagaaaaa gaatccttgg taaaatggga agacccgacc aaaatctcac
      601 acgtcttctt tcatagtctg atcgttgatc cagctaaggc ttttcatacc cagcaagcac
      661 aaggctataa agattatatg gtcactattt ccgaattcaa taaaaccatt gatcagttat
      721 ataaaaataa ctatgttctt gttaatctaa atggattagt caaaaaagga acagatggaa
      781 aactgacctt tacaggtgtt tctcttccag aaggaaagaa accgttaatt ctttcgcaag
      841 acgatgtcag ctattatgaa tacatggaca attcaggatt tccaagtaag ttgattgtcg
      901 ataaacagaa ccaaatcaag aatatttata tcgacaataa gaaggaaact gttggtgact
      961 atgatatggt tccattgatc gattcattca ttaaaaaaca tcccgatttt tcttatcaag
     1021 gcgccaaagg aacattagct ttaacaggct ataatggtgt attaggttac cgtacatcaa
     1081 aatcagaata tggtgataac gaaaaaacaa acaaagaaat cgaagcagcc aaaaaagttg
     1141 ctgatcaatt aaagaaagat ggttggagtt ttgcttctca tacttgggga cacttgaata
     1201 tgacacaagc ttctttagcc gatatccaac aagataatga acgttggcaa aatgaagttg
     1261 cacctatttt aggcaaaaca aatattttga tctacccatt tggcgctgac atcagcgatt
     1321 ggcaacctta ttctgaagca aatcaaaaat ttgcttactt gaaacaacaa ggatttgata
     1381 tcttctgcaa tgtcgatgcc tcaactcctg catggggaca actaggaact gactactacc
     1441 gtaacgccag aatcaatatt gacggtatcc gtttcgaagc tgacctaaaa ggagaaaatc
     1501 caatacttga tcaatttatc aacgtcaaag aagtgtatga ccaaaaagat cgaggataga
     1561 ggttgtgaaa gaagcgtcca gctccgccga caataagaat gaatttttct aaacagcttc
     1621 tcatgttttg aaaaatccag acttatttca aggagttggg ctttgaacac cgtgaataga
     1681 ggttgtaaaa agagcgtttt gacttattgt tgaaggtcag ctctttgaac accgcgtgtt
     1741 gggttgtgaa aaggctggtc tgcatcaagc aataagaatg aacagagaaa aacagcttct
     1801 tgaataccgc agataggttg taaaaaaacc gttttgacct gacgataacc agtaaaacag
     1861 aggtgagacc attgctaaaa gtagcagtgg tctcatctct ttttttgcac aagtcatatt
     1921 tgattgtttc gttattttaa tcaggataaa gttctttttt ttcatgctaa agagtgattc
     1981 tttcttctcg attttcactt tcttcgttta acttttggta aatacttcta tcagaaaaag
     2041 ctctaggtag acacacgaaa gcagataaaa agcctaactt ttgcaacagt ttatgctgtc
     2101 gttcaaagtt aggcttttac agtttataaa tagggttctg ttgcaaagtt ttccaaaaaa
     2161 tctattttag tgtaaaattg agaaaaaaga cagagaggac agagtaatga atcattttaa
     2221 aggcaaacaa ttcaaaaaag acgtcattat tgtcgctgtt ggttactacc tgcgttacaa
     2281 tctaagctat c
//
LOCUS       DANJMA010000136         2227 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000136 DANJMA010000000
VERSION     DANJMA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            5..397
                     /locus_tag="PWU31_002764"
     CDS             5..397
                     /locus_tag="PWU31_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1085217.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            398..1837
                     /locus_tag="PWU31_002765"
     CDS             398..1837
                     /locus_tag="PWU31_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL1085218.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1967..>2227)
                     /locus_tag="PWU31_002766"
     CDS             complement(1967..>2227)
                     /locus_tag="PWU31_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085219.1"
                     /translation="PKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVR
                     RREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          888 a          195 c          383 g          761 t
ORIGIN      
        1 cctattgaaa ctattgttta ttgatgataa tatggttttt gatattgata atttaaaagg
       61 atttcttaat gataccagtt catttgggtt tatagctaaa gaaaataata aaattatagg
      121 atttgcatat tgctatacac ttttaagacc tgatggaaaa acaatgtttt atttacactc
      181 aataggaatg ttacctaact atcaagacaa aggttatggt tcaaaattat tatcttttat
      241 taaggaatat tctaaagaga ttggttgttc tgaaatgttt ttaataactg ataaaggtaa
      301 tcctagagct tgccatgtat atgaaaaatt aggtggtaaa aatgattata aagatgaaat
      361 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
      421 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
      481 aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat cattaaaaaa
      541 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
      601 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
      661 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
      721 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
      781 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
      841 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
      901 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
      961 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
     1021 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
     1081 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
     1141 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
     1201 attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc
     1261 tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca
     1321 aatgcacggt ttagattata cagatattag tgaatgtact attgataata aacaaaatgt
     1381 attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa
     1441 agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa
     1501 gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt
     1561 aactggaata attgattttg gagattctgg aattatagat gaatattgtg attttatata
     1621 cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat
     1681 gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta
     1741 tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta tcgaaaatgg
     1801 tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat gaaaagctat
     1861 tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag gaagtgagtc
     1921 aagtccagac tcctgtgtaa aatgctatac aatgttttta ccatttctac ttatcaaaat
     1981 tgatgtattt tcttgaagaa taaatccatt catcatgtag gtccataaga acggctccaa
     2041 ttaagcgatt ggctgatgtt tgattgggga agatgcgaat aatcttttct cttctgcgta
     2101 cttcttgatt cagtcgttca attagattgg tactctttag tcgattgtgg gaatttcctt
     2161 gtacggtata ttgaaaggcg tcttcgaatc catcatccaa tgatgcgcaa gcttttgaat
     2221 attttgg
//
LOCUS       DANJMA010000137         2196 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000137 DANJMA010000000
VERSION     DANJMA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..914)
                     /locus_tag="PWU31_002767"
     CDS             complement(<1..914)
                     /locus_tag="PWU31_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL1085220.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWU31_002768"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWU31_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL1085221.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="PWU31_002769"
     CDS             2006..2176
                     /locus_tag="PWU31_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085222.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          743 a          384 c          421 g          648 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgttt
//
LOCUS       DANJMA010000138         2153 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000138 DANJMA010000000
VERSION     DANJMA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..917)
                     /locus_tag="PWU31_002770"
     CDS             complement(<1..917)
                     /locus_tag="PWU31_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL1085223.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVG"
     gene            1082..2095
                     /locus_tag="PWU31_002771"
     CDS             1082..2095
                     /locus_tag="PWU31_002771"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL1085224.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT          721 a          396 c          447 g          589 t
ORIGIN      
        1 tttccgacag caattggact gttcggaatc gtgaaaccag aagctgtcaa ttctttgaca
       61 taggagctca agaactcttc ttttaaggca tattgttttc cgttcaatgc gtagcagacc
      121 gtacgaatcg caaaattgct gtttccaaga tccactaatc caaagataga aggttcagtt
      181 tggagctctt cttggtattt ttcagctaat gtacgattta ctcgatcaat gatctcatag
      241 attttgtcat atccttcttc aggaacaatc cgaatatcta aaagtacttg catatttgca
      301 cgagagagat tgctgatcgt ggtgatgttt cgattcggga tgaaatggac agtaccatcc
      361 gttgctttca gctgaagggt acggatacca acagatacga cggttccttc aatcgttaag
      421 tttgctagct tgatgtagtc cccaacatcc atctgttgtt ccatgataat aaaaaatccg
      481 gtaataacat cactcatgaa cccttgggca ccaagaccga ttgccacacc tgcaattccg
      541 gcaccagcca aaagtgatcc gatcggcaca ccgatagcag ataaaattgc atagataaaa
      601 aagaaaccaa gggtataatg aaaaatagta tttaaaagac tatgtaaagt ctttaagcgg
      661 ctctcgttta gaatggcttt tttagaatac tgattaaaag acttatcaat cagatatttt
      721 cccacacggt gaagaatcca aaaaaggacg atcaaaaaaa tcagatataa acttttttgg
      781 ataaaggcag ccaaaatggc gtcccaatca atcccgtccc aaaaccgctg aaacgcattt
      841 actttgctga cagcttgatc tgtcaatgtc tcgccagaag agtctgtcgt accggcagtt
      901 gctaataatg aaaacataaa atcctctttt ctaaaatttg attctttctg tattgtacca
      961 agaagttttc aaaaaaggaa atagcaaaaa taactataat gaaggaaaaa agctgaaaat
     1021 aaaatgacaa ataatatgga taatgataaa atgtggagaa aataacgaag gtggtaagta
     1081 tatgcaaatc gattggaaca atctaggttt ttcttatatc aaaactcctt ggcgttttat
     1141 tgccaaatgg aaagacggac aatgggaaga aggagaattg acagaagata attacctgac
     1201 gattcatgaa ggctctccag cgcttcatta tggtcagcaa tgttttgaag gtctaaaagc
     1261 ttatcgtcga aaagacggca aggtcaatct ctttcgacct gaacaaaata gtcgacggat
     1321 gaatcaaagt gctagacgat tgctgatgcc aatggtccct gaaaagatgt tcgttgatgc
     1381 tgtcaagcaa gtggtaaaag caaatgaaga ttttgtgccg ccctatggaa caggcgctac
     1441 attatacttg cgccctttac tgattggggt cggtgaaaac attggtgtcc atccagctcc
     1501 agaatattta tttgtcgtct tctgtacgcc agtaggagcg tatttcaaag gtggactaaa
     1561 accaacgaat tttattgtat ctgattacga tcgtgctgct cctaagggga ctggtgcagc
     1621 aaaagtcggc ggaaattacg cagcgagttt acttccagga acagaagcaa aagaactgga
     1681 ttactcagat tgtgtttatc ttgatccagc cacacatacg aaaatcgaag aagtaggtgc
     1741 agcaaatttc tttggaatta caaaagacgg aaaattcatc actccgcaat cgccctctat
     1801 cttaccaagt atcacgaaat attccttgtt gcagttagct gaagaacgac taggactgca
     1861 agcaattgaa ggagatattt tcattgatga tctagatcaa tttgcagaag ctggtgcatg
     1921 tggtacagcg gctgttattt ctccaatcgg tgggatttat cacaataaca atcttcatgt
     1981 attttatagt gaaacggaag taggaccagt aacaaaacgg ttgtatgatg aattgacagg
     2041 tattcaattt ggagacatag aagcaccaga aggctggatt gaagtcgtgg aataatttca
     2101 aagtaatgag ttgaaaaaaa cgtaaaaaga aaaaatcagg aagagacttt aaa
//
LOCUS       DANJMA010000139         2112 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000139 DANJMA010000000
VERSION     DANJMA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            55..843
                     /locus_tag="PWU31_002772"
     CDS             55..843
                     /locus_tag="PWU31_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL1085225.1"
                     /translation="MVLLESMPDGYIYSNILLKLYLRSLKHEGKLMFNDRIPFNSTML
                     ATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSFIGKSSTEADRKRKYRKEI
                     EEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIENVTPSKKSKAKPVRHKYG
                     EYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSE
                     SKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            855..1124
                     /locus_tag="PWU31_002773"
     CDS             855..1124
                     /locus_tag="PWU31_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085226.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1129..1293
                     /locus_tag="PWU31_002774"
     CDS             1129..1293
                     /locus_tag="PWU31_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085227.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1286..1588
                     /locus_tag="PWU31_002775"
     CDS             1286..1588
                     /locus_tag="PWU31_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085228.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1585..1746
                     /locus_tag="PWU31_002776"
     CDS             1585..1746
                     /locus_tag="PWU31_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1085229.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1743..2048
                     /locus_tag="PWU31_002777"
     CDS             1743..2048
                     /locus_tag="PWU31_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085230.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          821 a          326 c          484 g          481 t
ORIGIN      
        1 aacaaacgat actactattt aaaactaaaa gagaactttt tcgatagcga cgagatggtt
       61 cttttagaaa gtatgccaga tggatacatt tattctaata ttcttctcaa actttattta
      121 agaagtctga aacatgaagg caaactaatg tttaatgaca ggattccatt caattctaca
      181 atgcttgcga ctattacaag acactctgtg ggagttgtag aaaaagcagt acaaatattt
      241 cgtgatttac aacttattga ggtattagat aacggagcaa tttatatgtc tgatatacaa
      301 agttttattg ggaaatcttc aactgaagct gataggaaga gaaaatacag aaaagaaatt
      361 gaggaagcaa aacagaattt aataacaagt ggacaaatgt cggacaaatg tccggacaaa
      421 actacaccag agttagagat agagttagag aaagatatag agttagagaa agatatagag
      481 aatgtaacgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
      541 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaatagaatt ccctaatgac
      601 taccaagagc gaatagaacg gctatctgag tattgtgaat catctggtaa gacttataaa
      661 aactatttgg caactattcg aagctgggca agaaaagaaa aaagtgaatc taagaacgca
      721 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgagtgggc aatcgaccaa
      781 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
      841 taagaggtgg gaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
      901 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      961 tcgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
     1021 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
     1081 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
     1141 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
     1201 gaagacaagt ggctcaacac gttccacggc ggacgtgaga gcgcacttga tcaagtactc
     1261 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
     1321 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
     1381 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
     1441 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
     1501 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
     1561 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
     1621 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
     1681 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
     1741 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
     1801 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1861 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1921 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1981 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     2041 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     2101 gattcagaaa ga
//
LOCUS       DANJMA010000140         2076 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000140 DANJMA010000000
VERSION     DANJMA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            793..1962
                     /locus_tag="PWU31_002778"
     CDS             793..1962
                     /locus_tag="PWU31_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1085231.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          761 a          287 c          310 g          718 t
ORIGIN      
        1 gtgttttttg tttgtaattc ctaattttgc tccactttta gataattttt aggtctattt
       61 aactcattag acattctatg ctcatttttc tccaaatttg tttcaactct ttcgtactta
      121 attttgcgta tttgaaaatt cttcttttag agtactttta ttacgatact agtatgatat
      181 atatctacag caaacagtta aaatcttcct aaaataagcg aatagcattc ctaaaaccta
      241 taaaattttt agatgaagcg acaagtggac aattaataaa ctacttgaac atgaaattat
      301 ggatcacctt tcaaatttag aaaatggaat tcttatactt attgcgcaac atttattgat
      361 tgcaaaatta tgtgacaaaa aaaactagta gaagaaagaa taatggcttg ataataaagt
      421 actacctatt aaaggtagta ctttatttaa tttagtataa attaacatac tactatattt
      481 taggtttcat attatataaa ttaataatat actaccattt gttggtagta taatttaaag
      541 gtaataagtt attcttttat tactatgttt gggttttata gcaaagaaag tttaataaga
      601 gtttggacct agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata
      661 acgaaatatc tacacagaca aaatgatagc gtaaaatatt ttgtgtaaat agatatttag
      721 ggtagaaaaa aagaaagtcc attctgtaaa attaaagtta ccacaaccga aattttatag
      781 gaggactttc tcatgcccaa ttttactaca gaaattatgg aaacactaat caataaaggt
      841 gatttagatg aattatttcg atgtcattta gaattagctg ttaattcatt attgcaggct
      901 gaattaacag cttttcttga ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat
      961 tcccgtaatg ggaattattc acgttcattc agaacagagt atggagaatt aaatttggta
     1021 attcctagag atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc
     1081 aatgactctt tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa
     1141 atctctgaat tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat
     1201 atgactaaaa tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga
     1261 tactctgtca tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa
     1321 gaagcagttt atattgtaat tggcattcga ttagatggaa ccaaagaggt tcttgggttt
     1381 agtcttgctc caactgaatc tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac
     1441 cgtggtttaa aagaggtttt attagtcgta actgatggtt taagtggcat taacgatagt
     1501 attcatagtg tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat
     1561 atcgctcata aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt
     1621 tatcaagcaa gttcaaaaga agaagcaaat aatcaaataa ggtttatgat agataaatgg
     1681 aaaaagcagt atccacgagt ggttaaattg ctcatgaatc ctgctatatt aaccttttat
     1741 aatttccctc cgtcaatcag aagaactatt tattcaacta atttgattga aggttttaac
     1801 aaacaattaa agaaatatac caaaagaaaa gagcaatttc ctaatgaaga atctctggag
     1861 agatttctag tatctcaatt caacaactat aatcaaaagt ttctatgccg agttcataaa
     1921 ggttttaaag aaatacatga tacattagaa tcaatgtttt aacttgatca acagaatggg
     1981 cttttccatt tacacaaaat tattgacgct cccgacaaaa ttatgatctt gcgtaaagtt
     2041 ataaaatatt aataaaaata ttttattaat gaaaag
//
LOCUS       DANJMA010000141         2056 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000141 DANJMA010000000
VERSION     DANJMA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            493..714
                     /locus_tag="PWU31_002779"
     CDS             493..714
                     /locus_tag="PWU31_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085232.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="PWU31_002780"
     CDS             715..972
                     /locus_tag="PWU31_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085233.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="PWU31_002781"
     CDS             complement(1108..1710)
                     /locus_tag="PWU31_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL1085234.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="PWU31_002782"
     CDS             complement(1777..1932)
                     /locus_tag="PWU31_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1085235.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DANJMA010000142         1991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000142 DANJMA010000000
VERSION     DANJMA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            128..1852
                     /locus_tag="PWU31_002783"
     CDS             128..1852
                     /locus_tag="PWU31_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1085236.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          781 a          241 c          348 g          621 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaaatta
       61 tatcttctta aacaatgata atattaaaat gcaagcgctt gtaaaaaata aggagaagag
      121 gtaaaaaatg aagaaaaaac tattaacttt atttcttgtg atgtctttct caatcgtttt
      181 gagtgtgtac tacaatacta ttgttttttc agaacaaata aactacacag aagaacagta
      241 taatatagaa aaagaaaaag ctttaaaagt tggttatagt gaagaacagt ttaagcagat
      301 tatggaaata cctacaaatt tatcaaatga aaatagtgaa acaagaatag taaattatac
      361 tatgacttct aatcaaacca aagtcattaa taaagcaatg gagcaaattg gaaagccata
      421 cgaatgggga gcatcgggac caaattcttt tgactgtggt ggtttagtga aatatgttta
      481 caaacaagct gtaaatattg aactgcctat gggaaccacc aatcaagagc aatacggtac
      541 agaggtaagt ttaaattcac ttaagcctgg tgatttatta ttttacggca atcgaggtgc
      601 aacttatcat gtaggtatat ataaaggtaa cggtgtaatg attcatgcac cgcaacctgg
      661 agaaactgta aaagaggtta acatacaata tttttatcct aactttgcaa aaagaatttt
      721 gcctgatgaa cctgactacc cttatattga ctataataaa atggtaactg ttacaaaagc
      781 ttggtcaata tggaatgatt tacaatttag tcatgaaatt aagaaagcta ttattggaga
      841 taattacaag atagggaagg tttatactaa tccagaaaat aataataaat atggagaaat
      901 attagtaagt aataaagtat atggatacat taatttttat gctgttaaag agttgacctc
      961 tgttcaaata aacagatatt taacttctaa agatagtggt caaccaactt ggggaaatct
     1021 agagtgtact ataagtaaag gacaaactac aaaagataaa atatattttg ttaaaggagc
     1081 atataattta ggggatggaa agtatctata ttctatttat aaagatcagg attcctctga
     1141 atggttaggg tatttaaaag cacatgttag tttagcctat actcctattg aagaaattaa
     1201 taaaaacgta actgttacta aaaattggtc gatttggaat aatctacaat gggaaaagga
     1261 aattgaaaag cctcagattg gttctgtatt ttcagctaga ttaaagttaa ccaatgtatc
     1321 gaataatgct gtctattaca agctttataa atctggaaaa ttttatggtt atatcaatgc
     1381 tgaggctgtc aaagacctta caacaacaaa gcttaataag tatgttacat ttagtgttaa
     1441 taatgaagat ttttggtctt ctttagatgt aaattattca aaaggaaaaa ctgaaagagg
     1501 ccgagtattt tacattagta tttcatataa taccgcagat aaccaaccga tttattcagt
     1561 atatacagat gaaacatgta cggaatggcg agggtattat aagggaaaca actttgaaga
     1621 cactcaaatt actatgcttg agaataaaag cgtgaaagta acaaaaagtg ggtatacagt
     1681 atggggagac ttgaattttt ggactaagtc aggaatatcg aacacaggag atatatacac
     1741 cactgaccga aaattttata attttaccaa caatgcttac tattatgaat tgaaaaaaga
     1801 tgggagggtc tatggctata taaatagtga agcagctgta gagatgaact aaaatattgc
     1861 tgtcccttaa tcttatgggg cagcaatatt tttatattat taatcatttt tctgaagcga
     1921 gggtagaaga attaatcaat agtttttctt gttttgtaat ataatagttt aattaccaaa
     1981 aattatttaa g
//
LOCUS       DANJMA010000143         1979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000143 DANJMA010000000
VERSION     DANJMA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(325..528)
                     /locus_tag="PWU31_002784"
     CDS             complement(325..528)
                     /locus_tag="PWU31_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085237.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="PWU31_002785"
     CDS             complement(728..1744)
                     /locus_tag="PWU31_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL1085238.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DANJMA010000144         1959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000144 DANJMA010000000
VERSION     DANJMA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            142..1017
                     /locus_tag="PWU31_002786"
     CDS             142..1017
                     /locus_tag="PWU31_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1085239.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPKR"
     gene            1207..1280
                     /locus_tag="PWU31_002787"
     tRNA            1207..1280
                     /locus_tag="PWU31_002787"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1241..1243,aa:Ile,seq:cat)
BASE COUNT          652 a          336 c          369 g          602 t
ORIGIN      
        1 aaaattacta ttactgcgat gagcctgtta acggctcttt ttttattacc cattaatacg
       61 tttgcttata ctattaatga cgagtataat ttagcgccga atcaaggaga ctccagatta
      121 gcaattccta acaaaattat tttgcacgaa actggaatag atgcaccagc aagaaacgta
      181 gccgccaaca tgaaaaataa ttataacgga agcaatcctt atactacaga tgttattggt
      241 gacggtggga ttgtttaccg tgtgggtgag caaggatatg tttcgtgggg agctggtaac
      301 gctaatcctt atgcgcctgt acagattgaa ttacagcgca catatgataa agcattgttt
      361 gaaaaaaact atcgagctta tattgaatat acaagagata gtgcaaaaaa atatggaatt
      421 ccattgactc ttgatcaagg aacttcttta tttacaaaag gaatcatttc tcatttgtgg
      481 gtgacaaatt atgtttgggg gaaccacaca gatccatatg gttacttatc gcaaatggga
      541 gttagcaaag aaaagcttgc ttatgattta gctcatggat ttaccgatga aaatccaaca
      601 acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacgctg
      661 acagatggaa caaatcacgc ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg
      721 catgtggctg gatggcacat tgctaactat aaatacgagt atattttcat tatggactac
      781 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaac
      841 caagcttata atacttctgg aaatgttggt tatcatgtat ctttcaatat gcgtaatttt
      901 cctagtaaga aagtctatgt aatgatgcgg gcaacgaatg atccagaggg aaacactaaa
      961 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
     1021 tagctcctcg ttgaggagca gtacataact atattgacaa ctataaaaat tattcgataa
     1081 aatagtgatg ttatcgcata tcttcactat cacccataaa tagtcacact ccaagctatg
     1141 cgataacagg tttgttgcca cacattctac tggttgattg tttatggctt tatgtggcaa
     1201 caaccagtac ccttagctca gttggtcaga gcagacggct cataaccgtc cggtcgtagg
     1261 ttcgagtcct acagggtaca ttaacgtagc catttgaatc gttctgtgtt agaatttttt
     1321 tgaagagtat tatacaagct aaagcttttc ttcattgcca ctcaaatgag tggctttttt
     1381 atgtatcctt ttatggatta atgaaaggat gtttcacata gttatacttc tgtatatttg
     1441 aaaagtttta ctttgatttt taaatagaaa gacatttggg ttaaattgtg agataataat
     1501 aaagaagagt ttaaagcgca ccccaaacca cttccccata agtgtgttac gctttaaact
     1561 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
     1621 gcttacaatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
     1681 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt
     1741 tcacaagtat ggtgataaaa ttcgttccgg gctacctttt taggtagcct actttaatct
     1801 ttataccttt ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgcta
     1861 cgtgcgtccg gcacgtcagt catgaatcgg ctacactaga ctagacagaa aaaataaggt
     1921 gtgtagaata ataaaaaaca cactggagga tttttcatg
//
LOCUS       DANJMA010000145         1930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000145 DANJMA010000000
VERSION     DANJMA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..435
                     /locus_tag="PWU31_002788"
     CDS             <1..435
                     /locus_tag="PWU31_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL1085240.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="PWU31_002789"
     tRNA            complement(759..842)
                     /locus_tag="PWU31_002789"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="PWU31_002790"
     CDS             1370..1870
                     /locus_tag="PWU31_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1085241.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          625 a          364 c          329 g          612 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat
//
LOCUS       DANJMA010000146         1890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000146 DANJMA010000000
VERSION     DANJMA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(152..346)
                     /locus_tag="PWU31_002791"
     CDS             complement(152..346)
                     /locus_tag="PWU31_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085242.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
     gene            594..1735
                     /locus_tag="PWU31_002792"
     CDS             join(594..822,822..1735)
                     /locus_tag="PWU31_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1085243.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          629 a          394 c          355 g          512 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgatttggta gaatctaact actaagaaaa ggacgtgtcc acgatggcaa
      601 aagccaagaa aaaatttgat gaagacttta agaagatgat cttagattta aaccagtctg
      661 gccaatcggt cgaagaactg gcagcgcaat atggtatcgc tacacaaacg atttatcgtt
      721 ggaagaagct tcatacaaag aatgtagcaa ccggcatgac tgaggcagaa atcctagcga
      781 tgaaaaagga aatggcccgc atgcaggagg aaaacactat cctaaaaaag gctttaacca
      841 tattcgctca aaagtaaaaa tgagtgatgt cttcgaattt attcaatcaa atgttcatga
      901 tcatgacgta aaacaaatgt gtaccgtttt ggaagcaccg aaatccagct actatgactg
      961 gaagaaaaag aaaccttcca agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc
     1021 aggaatctac tttgaaaaca agggtatcta tggtgcgcct aaaattcata agattcttgt
     1081 tggccgtggc gagaccttgt ccttaaagaa agtacaaata ctgatgagaa aattaggctt
     1141 gcgttcagtc accctcaaga agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg
     1201 acgtaaaaac ctgttaaacc aagattttac cactacgtcg atcaatcaaa aatgggtgac
     1261 agatattacc tatattcata ccctaaaaga tggctggacc tacctctcca ctattcaaga
     1321 cctccacaca aagaaagtca tcggttggaa gtttggcaag cagatgacga aagaattggt
     1381 tatcgaaacc cttgacaatg cgctcttaaa ccaaacaccc tccgagaact tgatcatcca
     1441 ctcagatttg ggttctcagt acactagcga agcctatgag gcaaagctga atgaattgaa
     1501 cattcggcac tccttcagtc gtaagggctg tccctacgat aatgccggta tcgaatcgtt
     1561 tcacgcctca ctcaaaaaag aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc
     1621 tgcttattta gcgttatttc aatacattga aggattttac aatcgcaagc gaatccatag
     1681 tagtatcaac tatttaacac caaatcagat ggaggagttg gcattgcaag cttagttcct
     1741 agctaccact tacatttttt tggcgagatt tccacagggt ttcaacttca tcgcttcgat
     1801 cagcggtcaa acggttttgg tttgaccgct ggtcgaaacg atgagatact cacccgctgg
     1861 aatctcgaaa aaattctcac aaatcgtgtc
//
LOCUS       DANJMA010000147         1783 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000147 DANJMA010000000
VERSION     DANJMA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..1161)
                     /locus_tag="PWU31_002793"
     CDS             complement(<1..1161)
                     /locus_tag="PWU31_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL1085244.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIA"
     gene            complement(1263..1616)
                     /gene="tnpB"
                     /locus_tag="PWU31_002794"
     CDS             complement(1263..1616)
                     /gene="tnpB"
                     /locus_tag="PWU31_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL1085245.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(1606..>1783)
                     /locus_tag="PWU31_002795"
     CDS             complement(1606..>1783)
                     /locus_tag="PWU31_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085246.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          494 a          374 c          320 g          595 t
ORIGIN      
        1 agcaataaat aatggacgca gttcttgttg ccgacagctt aatcgcttct caggagaaaa
       61 gtctttccat ttcttttcta ggacaaacca tttatccaat tgtttgactg cttgagctgc
      121 gggaatatcc gtgtttttct ttccttttgg aattgcttcg aaaaatttcc gccgaacatg
      181 cgcgaaacaa tagacaagtc gtacactttc cacggcatta tacccgctat agccgtcaca
      241 gtgtaaatac cctgtaaact ccttgagaaa agttttcgga acatctccag cacgactttc
      301 gtcgtggtga taataaacga tgggatgctg actgtctttt cctgtacaaa actgccagta
      361 ataggtatct gttttggcac tttcaatcac acgataactt gtttcatccc cgtgtagaat
      421 ctcttcttga ttaagataga ctcttaatcg ttctcctaaa gggtctaacg cgttttgcac
      481 tgctttaata tgccaattgg tgatattatc tctcgaaata tccaacccat gtgtttgttt
      541 ccaatagttc tcttgccgat aggcaggaac tttctgacca aatttcatcc ggatcgtttc
      601 cgccactaaa ctattggaac ctaagctatt agaaatcaaa ggtttaggaa cgatcgcttt
      661 tttaaaagaa gaaagtcctt ctttttcgca gcttttacaa caataggtat gttgataatg
      721 ggtgagacaa tccaacatag cctgatggaa acgaatttct tctcgtactt ttgtccgtcc
      781 gatatctttc atctgtgaac cgcaatgttc acaaaattgt tcttccaaag gaaggagatg
      841 ttctttctct gttataggta agtgtttgat tttgtctgct ttcgttccta caactcgttg
      901 atgtcctttt actggaataa tggtggcagc tgcttctgga acacttacag aaatttcttc
      961 atcactaaaa agattcaatt gcccattcgt ttcctcaaag agagtctctt tagagcgtcc
     1021 aaataatttg ttggttaaat aattgacctt ttcattcaat agttggattt cagttgtaag
     1081 attctgaatg gtttgttgtt gtgcttgatt ggttttatgg gcttcttgaa gcaactgaag
     1141 caacgtatct gtagtttcca aaatttttca ccaccttgaa tcgaactgat tttgttacag
     1201 aataccacaa gaaacgcaca agaaataggg gttcaacaca ttttttgtgt taacccccca
     1261 gcttaaaaaa ctcctttttt tcctggcaaa atagctcgag gttgatcgat acttaatcct
     1321 tctaacaacc atcgaacctg ttggttcgtt aaggttcgaa cttcgtcttt cgtccgaggc
     1381 cattttaact tgccgttttc tatccgctta taacagagaa gaaaaccatc accgtcccaa
     1441 tagagaagtt tgaaacggtc ttgacggttc ccgcaaaata aaaaaacggc atcttcgtaa
     1501 agctcgagtt gatactcttg ttgaataaga ctggcaagac catcaattcc ttttcgcaaa
     1561 tctgtttttc cgcacacaat ataaatatgt ttggtttgaa gtaaatcata aagcattgga
     1621 caacagttct ttcaaaatag cttgaagaat ataattgtta ataccgttat agatcaaaac
     1681 ttcagaacca ttctctcttt tgatgcgcat cgctaacttg gcagccgcgg gtttaccttt
     1741 ttctggtgca ctcttttcgt ttcctaattg gacaggaaca atc
//
LOCUS       DANJMA010000148         1735 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000148 DANJMA010000000
VERSION     DANJMA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            174..596
                     /locus_tag="PWU31_002796"
     CDS             174..596
                     /locus_tag="PWU31_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085247.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            606..809
                     /locus_tag="PWU31_002797"
     CDS             606..809
                     /locus_tag="PWU31_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085248.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1020..1604
                     /locus_tag="PWU31_002798"
     CDS             1020..1604
                     /locus_tag="PWU31_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085249.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          613 a          238 c          309 g          575 t
ORIGIN      
        1 tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa gaaaatttaa
       61 acgattatga cattaaaaat atattttatg aattttctag tatataactt gatttatctg
      121 ttaagattta ttaagaaaac gttttcttag ttttgatttc gaggtgatag acaatgaaaa
      181 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      241 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      301 taactccttt agatagggac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      361 ctgaaaatta tttaatagtt tctccattac ttgctctagc agcttctaaa acgatacgag
      421 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      481 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      541 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      601 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      661 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      721 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      781 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      841 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      901 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      961 tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt
     1021 tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca
     1081 gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta
     1141 atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt
     1201 ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga
     1261 atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt
     1321 ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga
     1381 tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc
     1441 gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta
     1501 cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt
     1561 tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg
     1621 ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg
     1681 tctactgata acttattctc aaatgttgta aggattttat tcatttgtta atatt
//
LOCUS       DANJMA010000149         1708 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000149 DANJMA010000000
VERSION     DANJMA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            288..695
                     /locus_tag="PWU31_002799"
     CDS             288..695
                     /locus_tag="PWU31_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085250.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVNLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="PWU31_002800"
     CDS             709..1110
                     /locus_tag="PWU31_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085251.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="PWU31_002801"
     CDS             1112..1483
                     /locus_tag="PWU31_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085252.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..>1708)
                     /locus_tag="PWU31_002802"
     CDS             complement(1519..>1708)
                     /locus_tag="PWU31_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085253.1"
                     /translation="SLLINLYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDH
                     RRYDYIKYTFVDPERYKD"
BASE COUNT          637 a          220 c          295 g          556 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaaacttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagcta
//
LOCUS       DANJMA010000150         1676 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000150 DANJMA010000000
VERSION     DANJMA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            227..1396
                     /locus_tag="PWU31_002803"
     CDS             227..1396
                     /locus_tag="PWU31_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1085254.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          590 a          273 c          277 g          536 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccat ccatagttaa tttatcataa tatcccatat tttaaaataa
      121 aagatagcgt aaaatatttt gtgtaaatag aaatttaggg tagaaaaaaa agtccattct
      181 gtaaaattaa agttaccaca cagaaatttt ataggaggac tttctcatga ctaaatttac
      241 tatagaaatt atgcaaacac tactcaataa aggggattta gatgaattat ttcaggatca
      301 tttagagcga gcgattaatt cacttctaca agcagagcta actgcttttt tagattacga
      361 aaaatatgat cgaaatggat tcaattcagg aaattcccgt aacgggaatt attcacgtac
      421 atttaaaaca gaatatggcg agttgaattt aactattcct agagatcgga atggcgaatt
      481 ttctcaacaa acactaccag catataagcg aaccaatgat tcattagaaa cgaccattat
      541 ccaattattt caaaaaggca ttactatggc tgaaatctcc aaattaattg aaaaaatgta
      601 tggtcaccac tatacgccac aaacaatctc caacatgagt aaattggtgg ctgaagatgt
      661 tttagctttt aaagaaagaa cgttagaagc caattattcc gttatattta tggatgcgac
      721 gcatatccct gtgaagcgac agactgtttc gaaggaagca gtgtatatta cgattggtat
      781 tcgtttggat ggaaccaaag aagttcttgg gtttactatt gccccaactg agtctgctta
      841 tatttggaaa gaagttcttc aagatcttag aaaacgtggg ttagaagaag ttttattagt
      901 agtgacagac ggattaagct gtattgaaga aagtatccat agtgtgtatc cgaatgccca
      961 atttcaacaa tgttgtgtgc atgtatctag aaatatcgct cataaagttc gtgttcgagg
     1021 tcgaaaagaa atttgtgagg atttcaaatt ggtttaccaa gcgaattcaa aagaagaggc
     1081 attggatcac atcgacttta tgactaggaa atggaaaaag cagtatccaa gagtcgtcaa
     1141 tttactcttg aatcctgccc tattaacctt ttataatttc cctcacgcca tcagacgaac
     1201 aatttattcg acgaacctga ttgaaggctt taataagcag ctaaaacgat atactcgaag
     1261 aaaagaacaa ttccctaatg aagaatctct agagagattc ttcatttctc aatttaatca
     1321 atataaccaa aaatttttag gtagaattca caagggattt aaagaaattc aggatacatt
     1381 agagtcgatg atttaactgt aattaacaga atggattttc catttacata taattcttga
     1441 cgctaccata atctaaataa ccatcatatt catctactac ttatataatt gagttttatt
     1501 aaacttttta agtttctatt ctatcaaata tttaaacaat ctctgcatcc gaaaagacta
     1561 cctcgtatag ttttactaac aatattttta ttagcgtgtt caaatcctga tttcttgtat
     1621 caattatttt ctgttcactt tcaataagaa aaaagtcttt acttccatgg ataata
//
LOCUS       DANJMA010000151         1603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000151 DANJMA010000000
VERSION     DANJMA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            90..1556
                     /locus_tag="PWU31_002804"
     CDS             90..1556
                     /locus_tag="PWU31_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL1085255.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          445 a          292 c          333 g          533 t
ORIGIN      
        1 tttatatttc ttaaataagt tttttagtta aatttttcgg taaagatgta taatattttc
       61 tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg
      121 ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt
      181 acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac gggggacttg
      241 gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt
      301 ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct
      361 tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta
      421 cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt
      481 ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga
      541 ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg
      601 gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat
      661 tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga
      721 atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg
      781 ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg
      841 tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga
      901 cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc
      961 ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg
     1021 ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat
     1081 cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac
     1141 ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat
     1201 tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc
     1261 cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag
     1321 ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta
     1381 tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca
     1441 caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta
     1501 tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa
     1561 tgaacataaa aacaggaatt gcttatttgc agcagttcct gtt
//
LOCUS       DANJMA010000152         1592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000152 DANJMA010000000
VERSION     DANJMA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(212..1252)
                     /locus_tag="PWU31_002805"
     CDS             complement(212..1252)
                     /locus_tag="PWU31_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1085256.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT          462 a          256 c          226 g          648 t
ORIGIN      
        1 attttgaaat aagactctat tattacaagt tcatccgttg taagatgggt ataggtcatt
       61 tgtactcact ccttataatt ctttggtcgg aactattttg agtgtagcac aaatgacttt
      121 tttagttgtc tagcttaatt ttacaatcgg cgaatcataa aaaagttcta atttaaaatt
      181 gatcattttt atagtgatcg ctttttatca actactctag ccaattttcc gtactaactg
      241 ttggtaattc gttaaatgtt tctctttgtt cgtttgcaag ctttgccgca atagcttttt
      301 cgacaaacca attttttaca taaacgaaaa ttagattctg caaattgttt actttttcag
      361 ttttttgttt ttgataagct ttcagcaaag tcatatataa tcctttgtca gcatcaattc
      421 cataagattc cagttcttca taaacaacaa ctgtttgctc taattcttca gcttttttct
      481 ttgcattatg aatagtttgg acggtttttt gagcgtcttc gtaagtacta gaaaatgttt
      541 ttatcaagtt taatacattt tctggtacaa atgtatttat actgtcctct ttcatgatgt
      601 cgacgaagtt atctagtaaa acttgatttt gtaactgatc agtttcagtg tctattatgt
      661 gtctattagt gtctatagaa ttagaataga atagattgtc tgcgattttc gcaattacgg
      721 ttgagtcctt gatatgactg gattttgccg tatttgcgat tttcgcaatt acggtggtat
      781 cattgatttc tcgcggtttt gccgtatttg cgattttcgc aattacggtg gtatcagtat
      841 tgtcttcctc ttccatgatt ttaaagacgt cttgattcgt tacttctggc ttgcctaagt
      901 acaataaatt aggcatggat tcaatcttac cgtcacgttt atttactctt cctctcttct
      961 gtttcagaag acctgctttt tctaattcct ttttgatttt agatacttta ccttcgcggc
     1021 attgtaacaa tttcattaat tcttcaactg tgaatatgaa aaatatatta tcatcagaat
     1081 ctacccagtt atttttgact gaataatcaa atcgatcttt aagtagcata taagcaattt
     1141 ttgcatcatt tgatagatcc atgtattttt cgttagtaaa aaatacctta ggcatttgat
     1201 aatatttttc acgataaatt tcttttgatt tgtaataatt aaaatcattc atagccaatc
     1261 ctctttctgt aggatagcgt tttgttcaaa actatgttac aatgaattaa acaaatatat
     1321 ctatccacta tatatttttc tactgctgat aagtcgccaa actttgttaa agcagtagtc
     1381 tttttttatc tttttttagt ttaccttaaa aaataaatta aagcaaaccg ctctatttca
     1441 aggtttaagc tcatagtaac tatatcacga tattacaata atattatatc acgatatcgt
     1501 aatattacaa tatgaagata agcgacttat cagctttgat actactgatt tttataaaaa
     1561 aaatatcacg atattatgat atcgtgatat tg
//
LOCUS       DANJMA010000153         1589 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000153 DANJMA010000000
VERSION     DANJMA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            89..940
                     /locus_tag="PWU31_002806"
     CDS             89..940
                     /locus_tag="PWU31_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085257.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            961..1244
                     /locus_tag="PWU31_002807"
                     /pseudo
     CDS             961..1244
                     /locus_tag="PWU31_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          595 a          207 c          320 g          467 t
ORIGIN      
        1 acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta
       61 aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg aacacacctg
      121 gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat tatggccatt
      181 aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt actcgattgg
      241 agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag catcagacat
      301 ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta ctcaattagt
      361 catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc tattcgattt
      421 aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat ctggaagaac
      481 aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg gacacatgta
      541 tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga aacttattaa
      601 acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg gttatgagta
      661 caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc acacgagtat
      721 ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg aaatgaaagc
      781 aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc tacgatatgg
      841 catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat acattttttg
      901 gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa tcgtttgaaa
      961 atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac cttaaagaag
     1021 gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa aacgggtacg
     1081 atgaacattg aataccgaac agaatattta gaattaacga atcaaactga ttttttgttg
     1141 attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa ttgattgatg
     1201 aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct ttatttgtaa
     1261 agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat
     1321 tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag tgaaaggata
     1381 gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc ttttcgctat
     1441 cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga tgagaaaatc
     1501 gtaagagaaa ttgatcgaag atgtgacgaa cggttctgtt gcaaagtttt aaatctacta
     1561 tcaaataagg tagaataata gaaaaagat
//
LOCUS       DANJMA010000154         1579 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000154 DANJMA010000000
VERSION     DANJMA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..620)
                     /locus_tag="PWU31_002808"
     CDS             complement(<1..620)
                     /locus_tag="PWU31_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085258.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKN"
     gene            complement(630..1367)
                     /locus_tag="PWU31_002809"
     CDS             complement(630..1367)
                     /locus_tag="PWU31_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1085259.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
BASE COUNT          475 a          307 c          245 g          552 t
ORIGIN      
        1 aagtttttag tataaatcgc tttgatttta tgatccgtac ctagactttt ttcatgacgt
       61 aatgtgttat tatcttttag aaactcacca tgaaatcgat ctagaataga tccctctttt
      121 ccaccaaaga attctaaaaa attcgccttt tctatcttca cattagcaag cgtatctact
      181 aatgacgaga aagaaaactg cgaagggata gctggtttcg ctaaaacttt tgcgttttgc
      241 catgcctgag tagcagtgat tttttctgtt ccgctgtcat atttattcaa caccgatttt
      301 cttatatcat tgaaaatagg ttcagctttt acttctatcg tattgcctat tacagaagtt
      361 tttgcataat aaatccgtag acgctgtttt gctcgatttt catctacata acactgaata
      421 atacgtcctt ctacaatcaa atctgcatta gttccgttta ttgaataagt accctgaaat
      481 atctcggctc cgtttagttt attgctaaca gtagctgtta accagtctga caaagcgcct
      541 aaaccttgcg tatcatataa atgttcagct aaattattcg cgtcattttt atcgtaaata
      601 gttagtaaat tatcgatcat ctatttcacc taccttaacc cattacgata aatttgtatt
      661 ttgctcaaac cagtacaatt aaaatgatta atatccactt gcaacgttgg atattgcatg
      721 gtcttcattt tgttggaccg atctaaaata tctccgtccg attgctcttc gtagcaaagc
      781 atcaaatcac tatcaatgac tatgtcagtt cccactacta agccttcaaa actaaacaca
      841 taatcattta aggtgaattg gcatgaagta gctgaaggag tgatgataat ctttggaaaa
      901 ctttcttcta aactattatt cagcaagtta aatgactgtg gtttatctac ggttataggt
      961 acatcttctt gaactcttgc gaatggtttc gcagtgatat ttacatcgaa ctctccccat
     1021 tcaacaatat cgttttctgc atccccaata tcgatagtct ggataacata atagacgttg
     1081 ggatcatcag agaattctaa tttctttgca tagtttaacc aatgacgcat gatataaaac
     1141 gattgcttga atgcttgatg gtcttccaca tcttctaaat agttatagtg caatgtaaac
     1201 gacatgtctt caaacgagta atcttgtact aagccaccta gccgtcctaa aacagaagtt
     1261 tcaactctct gtctttttgg agaaggtatg gttggtcttt ccgctaaagc caatttatgc
     1321 aaataatcag gaaatccatc gattatagaa tgtatacaat cagtcatttt ttcacatcct
     1381 ttttaaaact aaaaaaacag gagaaatact ctcctgttta acgccatgcc gaagcattat
     1441 cattttgaac ttttgtaatg ctatcaatga tttgttgagt tgtttgcttc atagtaacct
     1501 catctgcgtt accatcaatt gtgaaattga attcgtagtt attcacaggt tgaatcgttt
     1561 gtgccctaga tgaaactga
//
LOCUS       DANJMA010000155         1555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000155 DANJMA010000000
VERSION     DANJMA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..484
                     /locus_tag="PWU31_002810"
     CDS             <1..484
                     /locus_tag="PWU31_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1085260.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1092)
                     /locus_tag="PWU31_002811"
     CDS             complement(691..1092)
                     /locus_tag="PWU31_002811"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085261.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1123..1221)
                     /locus_tag="PWU31_002812"
     CDS             complement(1123..1221)
                     /locus_tag="PWU31_002812"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085262.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            1319..>1555
                     /locus_tag="PWU31_002813"
     CDS             1319..>1555
                     /locus_tag="PWU31_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085263.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPR"
BASE COUNT          499 a          299 c          304 g          453 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaat aaaggcgatt tgaatgaatt
     1381 attccgtgtc catttggaaa aagctgtcaa tacgcttctc aaaacggagt taacggcttt
     1441 cctcgattac gaaaagtacg atcgcattgg ttttaacacg ggtaattctc gtaacggctc
     1501 ctatgaccgt acggtcaaga ccgagtacgg ggaacttcat ctccagattc cgcgc
//
LOCUS       DANJMA010000156         1485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000156 DANJMA010000000
VERSION     DANJMA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            368..922
                     /locus_tag="PWU31_002814"
     CDS             368..922
                     /locus_tag="PWU31_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1085264.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1176..>1485
                     /locus_tag="PWU31_002815"
     CDS             1176..>1485
                     /locus_tag="PWU31_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085265.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          596 a          153 c          256 g          480 t
ORIGIN      
        1 atgaaaagtt tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg
       61 agttcttagt atattgttca aacttaaatt tcatataatt taattatgta aattagctat
      121 tctaagaact tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca
      181 tataagtaag gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga
      241 tataaaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt atatatataa
      301 gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt taagaaggga
      361 aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca
      421 gttttggcta agaagaacaa gtaatccaga aagagatagt tttttatttt tgtttggaat
      481 caataatggg ttacgtatgt cagatattat aggattaaaa gttggagaca tacgaggtaa
      541 aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa
      601 tttgagagaa gagattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc
      661 aagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat
      721 agcagaaaaa ttaggaagag atgatatagg aacacataca ttaagaaaaa cttttgggta
      781 tcactactac aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc
      841 gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaatagggg catcgttacg
      901 tggttttaaa ttgggaggct aattgagata ttaggatata ttttttgtgt ataatgaaaa
      961 cttagaaggt gagattaatg aagataactg aattttcgga agtatttaaa acgtttagta
     1021 acatttacaa tagaatacca gagatagaac aaaatgttgt agttgataat gattattata
     1081 tagagaatga ttttaataaa ccaagaaaat atgacaacct atacaatttt atacacaata
     1141 gaacaacaat aaataacaac agtaacactc gagttttgat taattcagca tattttcata
     1201 ttaaatattt aaagaaatat gagtatgaag aagttgtatt agattatggg ctaaatagac
     1261 agtcattttt aatttatgca ataaataacg gaaatttgcc aagcaataaa aagaacataa
     1321 ttattgaaac atatgcctat ctggaggggg gatctaaaaa actaattaag tcatgtaata
     1381 tagagtatca attatcagta ggagaaatta aatgtgtttt cgaagaagat ttagaacttg
     1441 acttaaaaaa aatttttgcc agcattcatg attatatagg tttga
//
LOCUS       DANJMA010000157         1460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000157 DANJMA010000000
VERSION     DANJMA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            74..484
                     /gene="spx"
                     /locus_tag="PWU31_002816"
     CDS             74..484
                     /gene="spx"
                     /locus_tag="PWU31_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL1085266.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            498..854
                     /locus_tag="PWU31_002817"
     CDS             498..854
                     /locus_tag="PWU31_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL1085267.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          270 g          439 t
ORIGIN      
        1 tttattagct aataattggt attttaaaag aaattactgt agaattggat tataattaaa
       61 aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc
      121 aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc
      181 gttaacagaa agtgagatca aaaagatctt agcagcgact gaaggaggag ttcaagatat
      241 tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt
      301 aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat
      361 tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg
      421 tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc
      481 atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc
      541 tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag
      601 aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag
      661 aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg
      721 taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt
      781 tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta
      841 tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta
      901 acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac
      961 tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta
     1021 aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca
     1081 aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac
     1141 atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa
     1201 aaacttcaaa aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg
     1261 taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct
     1321 ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggggaa cactcctgat
     1381 gaagtagacc tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat
     1441 tcaattcaag acaacaaaca
//
LOCUS       DANJMA010000158         1429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000158 DANJMA010000000
VERSION     DANJMA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..>1429)
                     /locus_tag="PWU31_002818"
     rRNA            complement(<1..>1429)
                     /locus_tag="PWU31_002818"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          298 a          427 c          280 g          424 t
ORIGIN      
        1 actcctcttc agacttactg cttggacaga catttccgat cgtctgcatt ccttagcctc
       61 ctgcgtccct ccattgctca aacaaaaaca acgggtacag gaatatcaac ctgttatcca
      121 tcgcctacgc ctgtcggcct cggcttaggt cccgactaac cctgggcgga cgagccttcc
      181 ccaggaaacc ttagtcattc ggtggacagg attctcacct gtctttcgct actcataccg
      241 gcattctcac ttctaagcgc tccagcagtc ctcacgatct gccttcaacg cccttagaac
      301 gctctcctac caatacacct aaaggtgtac tccacagctt cggtaatatg tttagccccg
      361 gtacattttc ggcgcagggt cactcgacta gtgagctatt acgcactctt taaatggtgg
      421 ctgcttctaa gccaacatcc tagttgtctg tgcaacccca catccttttc cacttaacat
      481 atattttggg accttagctg gtggtctggg ctgtttccct ttcgactatg gatcttatca
      541 ctcacagtct gactcccgga tatgaatgaa tggcattcgg agtttatctg aattcggtaa
      601 cccgagatgg gcccctagtc caaacagtgc tctacctcca tcattctcaa ttccgaggct
      661 agccctaaag ctatttcgga gagaaccagc tatctccaag ttcgtttgga atttctccgc
      721 tacccacacc tcatccccgc acttttcaac gtacgtgggt tcggtcctcc agtgcgtttt
      781 accgcacctt caacctggac atgggtagat cacatggttt cgggtctacg actacatact
      841 caaacgccct attcagactc gctttcgctg cggctccgtc tcttcaactt aacctcgcat
      901 gcaatcgtaa ctcgccggtt cattctacaa aaggcacgcc atcacccatt aacgggcttt
      961 gacttgttgt aggcacacgg tttcaggttc tatttcactc cccttccggg gtgcttttca
     1021 cctttccctc acggtactgg ttcactatcg gtcactaggg agtatttagc cttgggagat
     1081 ggtcctcccg gattccgacg gaatttctcg tgttccgccg tactcaggat cctcctaggt
     1141 gtcttccaca tttcgtctac ggggttttta ccctctttga ctgacttttc caagtcattc
     1201 gactatctga aagaactacc atattggagt cctacaaccc caacaagcaa gcttgctggt
     1261 ttgggctttt cccgtttcgc tcgccgctac tcagggaatc gaattttctt tctcttcctg
     1321 caggtactta gatgtttcag ttctctgcgt ctacctcgaa tgtgctatgt attcacacaa
     1381 tcgtaacatc ctattaaaga tgttgggttc ccccattcgg aaatctctg
//
LOCUS       DANJMA010000159         1425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000159 DANJMA010000000
VERSION     DANJMA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..1216)
                     /locus_tag="PWU31_002819"
     CDS             complement(<1..1216)
                     /locus_tag="PWU31_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1085268.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKI"
BASE COUNT          516 a          224 c          166 g          519 t
ORIGIN      
        1 aaatcttaat cggtttaaca tttgttaaaa ataaatcata ataatctaac acataataat
       61 aatccaaaaa taattgtgcc cagttttcta atttctttga tacatccaaa tccgcatttg
      121 cttgtaattc ttttttgagt ttttccatta tgtgataagt tcgaggttct tcttctttca
      181 tttcttcgat atacgaacga ttaaaaaaaa gatctggatt accataaaat agataacttc
      241 tttcatgaaa tgcaataaaa ttgaatatga gttctggatc ctttatatct aaagaaaaac
      301 ggaacatgct ttgtaaaact ttctccaaat tctcaactgc tcgataagaa caagtatttt
      361 gtcgctgatg tgcatatgca tgactatttt catatattgc atttccatca attactccaa
      421 aagaataaat tattcgataa aggaaacaca gttcatcgct gttaaaagaa agattgattt
      481 gttttgacca cttcttcaaa agattaaaat gcggatcatc tgagataact gatttatatt
      541 cattatctac tcgtactttt ctaaatgaac ttctttctat attaatggct aaccaaaaag
      601 aaaatacacg tttttgaaaa atagttaacg taatatcata aatcccctca aaagcaacta
      661 tatacttctc tatttccttt tgttcgatat gaaatggcca cttatcacta ctttcacgtg
      721 tagaattcca atagaaataa taaaaaaagc tacgaatgtt tgcctctgat gcattcaatt
      781 ctaactgaaa tggatgaaat gttatatgat aacgcctaaa atatggaatt aagagcttcg
      841 catatctgaa aacagtagat acactagtaa attgctcttt agcaaaatct tttagaacac
      901 ttctaggata tttatacata aaatcaatca aatgataatt ataagaagct ttataaaaat
      961 gaagataaat attataaata ggaaaatctt tctctttttt tatgaaaaat cgtttattat
     1021 cttcattttt cagaaggata ttaccattat attgcttaaa taaatcctct agttcttcta
     1081 tatagtgtaa gactgacttc tccgtaacat taagtaaatt tctaagttct tccattgcac
     1141 accaattgtc tttttctaac aaataatcta ataaaactac ttttttgaaa gatgttttag
     1201 ttaaaaaagt ttgcatcatt tcacctcaat aagttacttt tgtttatttt aatcaaagaa
     1261 taaacttctc tcgttcgact ataactgatc gaacaaaaga agccttttaa taaatataat
     1321 ctcacttaca tacataggct agttatttgc ttctagctta tgataagtat ataaacggat
     1381 atcgatttca taattgtttt atcttaaaca tcatagctgt aattg
//
LOCUS       DANJMA010000160         1424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000160 DANJMA010000000
VERSION     DANJMA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(150..1337)
                     /locus_tag="PWU31_002820"
     CDS             complement(150..1337)
                     /locus_tag="PWU31_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL1085269.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DANJMA010000161         1423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000161 DANJMA010000000
VERSION     DANJMA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            98..1390
                     /locus_tag="PWU31_002821"
     CDS             98..1390
                     /locus_tag="PWU31_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1085270.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DANJMA010000162         1422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000162 DANJMA010000000
VERSION     DANJMA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PWU31_002822"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PWU31_002822"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL1085271.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNADKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          497 a          185 c          236 g          504 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ctgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta ta
//
LOCUS       DANJMA010000163         1406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000163 DANJMA010000000
VERSION     DANJMA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..416)
                     /locus_tag="PWU31_002823"
     CDS             complement(<1..416)
                     /locus_tag="PWU31_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1085272.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTS"
     gene            1092..1187
                     /locus_tag="PWU31_002824"
     CDS             1092..1187
                     /locus_tag="PWU31_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1085273.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          461 a          281 c          221 g          443 t
ORIGIN      
        1 aacgacgtga aagcagataa ttcaaatcta ctaaattcag ctgttaaacc attaataata
       61 tattctaggt ccggattaac actgacttca acaaactttt ttgttttatt gatttcaaaa
      121 ccagtaaaca aaacaaagta gcgaatagaa tcctcatctt cagtacggta agtaagatta
      181 agcatatctt tatagagctt ttctaaatca ccgacaaagg cttttgtagc agtcatctta
      241 taatcactta actcttttaa ttcctcgaaa gaaaatctaa cttttttcaa accacgatct
      301 ttcatacgag cgcataatgc aaaaaataaa tccatctgct tagcattgaa atttttcagc
      361 ggtactaaat tcaattcatt ttgatatttg actgttaatt ccgttttttc atccattcct
      421 ttcttccccc ccttacaaat aaataataac ctttaacaac caactcgtaa ataggttggt
      481 tgttattata caaaaggata ttagtactca gataaaaagt tgttagtact cagataaaaa
      541 gttgttagta ctcagataaa aagttgttag tactcagata aaaagttgtt agtactcaga
      601 taaaaagttg ttagtaccct tctgtatccg ttgggagagt aaggctcaac tgccccctaa
      661 aaagggttct aaaaaatctt taaaaaataa ataaaataaa cgcgcgcgcg cgagcaaaat
      721 ttcccgccaa aaaatcaccc actttttaaa ataaacaggt tcgcgcatga tattaaactg
      781 ccaatctagc tccctatggt cgctgtaaga aggagtgatc gctacgctct cacattttac
      841 ttgcagcagt cgcctttagg ctccttcgcc aaaaataatt cattagggac tcctttgtgg
      901 cgtcctgaga gctcgtctaa gccgttctaa gacaagattc gataaaaact atgtccttcg
      961 ttctaaagcg ccttacaggc ggtcagggca agccctaaca accccaaggc aataaaaaac
     1021 ttaggcttat ttttgctttt ttattgtata attgattgtc gcctatccga cagtcttgcg
     1081 actggaagga ggtgtgtttc gtgttcacta actttatttg ctcccttctt gttgggcttt
     1141 taagcgcttt gttcagttac tggttgaata accgaaacaa gaagtaaaac ttaattaagg
     1201 cgactcagcc cacaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtgttt
     1261 cgtgttcact tatttgcaat caaattataa cagaaataaa tatttatttc aaaaatatcc
     1321 tacaaaagtc taaaatccgt aattatttct tatatgcaac atttacaaat aaagtcgctc
     1381 tgagagctcg tatttttcga tttaag
//
LOCUS       DANJMA010000164         1406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000164 DANJMA010000000
VERSION     DANJMA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..85)
                     /locus_tag="PWU31_002825"
     CDS             complement(<1..85)
                     /locus_tag="PWU31_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085274.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            360..902
                     /locus_tag="PWU31_002826"
     CDS             360..902
                     /locus_tag="PWU31_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085275.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1058..>1406
                     /locus_tag="PWU31_002827"
     CDS             1058..>1406
                     /locus_tag="PWU31_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL1085276.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          550 a          171 c          269 g          416 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa attgatgaag cagatcccaa
      181 agggctgctt catcaatttt ttcgtaagtg tccgtacact tactgtgctt gctcatatat
      241 taatcgatca tatactaatc gtgtaagaac aactttaagc ataatactta tattgttata
      301 ccatagtttg gaaaaataaa aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa
      361 tgttattgaa agtagctata aaatgctatt gtgtaaaaaa taaaggagga ataatttatg
      421 aaaaaatagt accaagagga gcatgcctat tacttgcatt atgtttgagc ttaagcgcag
      481 ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga aggacaatcc ataagttctg
      541 aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga aacttttaag ccatcagatt
      601 atacattgga ggcaaagaaa gaatatgttt atgaatattt agggcttaaa tttaaactct
      661 cagacaaatt tagaaattat atagctgata aaaagatagc gatgttagat gatcaaagtc
      721 ctatagataa agaactaaag tatgctattt tgacatttga gaaaatgaca gaagaacaaa
      781 agaatgcagt tatagaaaag atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa
      841 gaattgggac tattggtata tttgaaaaaa atacatcaga agaaaaaaat ctaaaattat
      901 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaatacg attgctattt
      961 aagcacaaat agcggagctg aaagtaatct tcttgatgaa ttaaaaagaa ctgagattca
     1021 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
     1081 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
     1141 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
     1201 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
     1261 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
     1321 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
     1381 ggcttcatat cctttcttaa tgccag
//
LOCUS       DANJMA010000165         1376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000165 DANJMA010000000
VERSION     DANJMA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(26..271)
                     /locus_tag="PWU31_002828"
     CDS             complement(26..271)
                     /locus_tag="PWU31_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085277.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            615..899
                     /locus_tag="PWU31_002829"
     CDS             615..899
                     /locus_tag="PWU31_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085278.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            902..1318
                     /locus_tag="PWU31_002830"
     CDS             902..1318
                     /locus_tag="PWU31_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085279.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          490 a          252 c          199 g          435 t
ORIGIN      
        1 cgattttaga gccatttaaa cccacttaat tatattctcc atgaaattcg ctccatgcgt
       61 caaaagctgc gtctgaaatt ccgccgtctt cgttcactag ttgcattaat ttttccaacg
      121 atcgttttct ttcgggtgtc atatttttat attccaacaa tttttcgtct gttaaatcga
      181 acttgattgc ttgaattgct tgctctaatt tttctaagtt ttgaaaatat tcgtaaggaa
      241 atttttccat tttttctttc tccagtctca caatttttct agaaataaaa tcacttcttt
      301 gccgtctata ctctcaaaaa aaatgtcgtt tgaaaaacta aataaatttt tgatcgtgca
      361 tttttaaatt tggttatcga ttacacgtgc agcactcccc cccgaatttt aaagagcata
      421 aggaaacgtg tacccacttt ctaaaaaaag tgggtagata ggctgtgcct atcacgtttc
      481 tcagaaaaaa tttgatatca ctttttcgcc atcgtgggta gcttatgtat agcattttgg
      541 caaaaaacag ctatacaaat atagtatttg tatgatatac ataaattata cataattgca
      601 aaaggagatt ttttatggac attttagttc ggaatattga tccaaagttt gttgcaacaa
      661 tagacaaacg ttgccaagaa ctcacgaata aaacaggaga aaaatggagt cgaaataaat
      721 atctaaaagt tttaatcgaa agtgattttg accgtcaact tacgacttac aaaaaagacc
      781 aattcgattt attgctcgat aaatttattg ctatccaaac agaaaatact caaatattaa
      841 gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct tatcggagga atgatataac
      901 catgagaaaa acttctgtcc aaaaacatgt tactttgtac cctagtcatg ttgacgtttt
      961 agattccatc atcgaaaaaa atccagatat caataactac tcggaagcca ttcgcttttt
     1021 atgtatgagt tatgaacccg ataccaacac gaccactacc agcaatcagg caaaactaaa
     1081 tgctatggga aaagaaattt ccatcatgac tgaaatcatc gcagaatttg ctgatttata
     1141 tctaaaagat acaggcgttc ttattggcac aaattctaaa gtatatcaag aagtgaaaga
     1201 aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc atcaaaaaga
     1261 aaccagtcaa acagtcactt ctagaaattt cgacccaaca aattttctaa aacgataaaa
     1321 aacagatagg agttattcct atctgttttt tatttagaag ccccatacaa tatgct
//
LOCUS       DANJMA010000166         1374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000166 DANJMA010000000
VERSION     DANJMA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            39..1340
                     /locus_tag="PWU31_002831"
     CDS             39..1340
                     /locus_tag="PWU31_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1085280.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DANJMA010000167         1364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000167 DANJMA010000000
VERSION     DANJMA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            82..>1364
                     /locus_tag="PWU31_002832"
     CDS             82..>1364
                     /locus_tag="PWU31_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL1085281.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DANJMA010000168         1364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000168 DANJMA010000000
VERSION     DANJMA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            82..>1364
                     /locus_tag="PWU31_002833"
     CDS             82..>1364
                     /locus_tag="PWU31_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDL1085282.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          463 a          255 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DANJMA010000169         1287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000169 DANJMA010000000
VERSION     DANJMA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            316..570
                     /locus_tag="PWU31_002834"
     CDS             316..570
                     /locus_tag="PWU31_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1085283.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="PWU31_002835"
     CDS             571..888
                     /locus_tag="PWU31_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1085284.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          448 a          208 c          256 g          375 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatg
//
LOCUS       DANJMA010000170         1235 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000170 DANJMA010000000
VERSION     DANJMA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>1235
                     /locus_tag="PWU31_002836"
     CDS             <1..>1235
                     /locus_tag="PWU31_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085285.1"
                     /translation="KLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKGYQKRTAEE
                     IKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQFPGATACL
                     GRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKRIESGQIEV
                     IKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANNIGI
                     QIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMH
                     KFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDKEAAERGKI
                     MNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWI
                     DKKDGKVMEEDTGRTLHLK"
BASE COUNT          482 a          205 c          270 g          278 t
ORIGIN      
        1 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
       61 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
      121 acggcagaag agattaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
      181 aaaaagcata ctcattctcc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
      241 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
      301 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
      361 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
      421 cgagaaatag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
      481 atcaaaaaaa cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
      541 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
      601 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
      661 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
      721 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
      781 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
      841 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
      901 tttggaatgg acacgttgga gaaagctatt ccttatattg cagcatggac gaagaatggt
      961 cagacgttgg atgataagga agctgcggaa cgtgggaaaa tcatgaatga tgtcagccgt
     1021 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
     1081 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagaa
     1141 caaaagaata ttgttcctgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
     1201 gaagaagata cgggtcgaac gttacatttg aagga
//
LOCUS       DANJMA010000171         1158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000171 DANJMA010000000
VERSION     DANJMA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            116..1135
                     /locus_tag="PWU31_002837"
     CDS             116..1135
                     /locus_tag="PWU31_002837"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1085286.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          407 a          183 c          260 g          308 t
ORIGIN      
        1 aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac aatggaaatt agtcccaact
       61 ttaagaaatg tatactataa tggaaattat caattattta tttggaggaa tatccatgaa
      121 acaaaaaatc gcagttcttg gtcctggatc ttggggcact gctttagcac aaacattggc
      181 agaaaacggg catgatgtcc gtatttgggg aaatgtacct gaacagattg atgaaatcaa
      241 taagtatcat acaaatcagc attttttacc tgatttgacg atcccagaat cgattatcgg
      301 atataaagaa ttggcagaag cagtggataa cgcagatgct attttatttg ttgttccaac
      361 aaaagcgatt cgttcagtcg cacaggaact tattggtaaa atgaatacta agccaatcat
      421 tatacatgca agtaaaggac tagaacaaga tacgcataaa cgaatctcag aagttttggc
      481 tgaagaaatt ccagaagaaa aaagacaggc aattgttgta ttatctggtc ctagccatgc
      541 tgaggaagtg gcagtacatg atatcacaac aattacagct gcaagtattg atcaaaaagc
      601 agcagcctat gtacaaaaac tgttcatgaa tgattatttc aggatttata caaataatga
      661 tgtgattggc gtagaaactg gtgcagcact gaaaaatatc attgcaattg gtgccggcgc
      721 tatccatgga ttaggattcg gagatgatgc aaaagcagca atcatgactc gaggattggc
      781 agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct ctgacattta tcggtttgag
      841 tggtgtgggg gacttgatcg ttacttgtac aagtgttcat tctcgcaatt ggcgtgccgg
      901 taacttgtta ggacagggac ataagctgga agaagttcta gaaaacatgg gaatggtcgt
      961 tgagggtgtg gcaacgacaa aagcagcagt agagctagct caacagctag gtgttgagat
     1021 gccaattact caaacaatct ataatgtttt gtataatgga gaagatatca aaaaagcagc
     1081 aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa ttttctcttc gttaaataga
     1141 tgatcaatag ttaatcgt
//
LOCUS       DANJMA010000172         1097 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000172 DANJMA010000000
VERSION     DANJMA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            170..439
                     /locus_tag="PWU31_002838"
     CDS             170..439
                     /locus_tag="PWU31_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1085287.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            432..689
                     /locus_tag="PWU31_002839"
     CDS             432..689
                     /locus_tag="PWU31_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1085288.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          426 a          123 c          195 g          353 t
ORIGIN      
        1 gaaaaagcag gatttgaggt accagcactc aaaaagtgac gtcacttttt atcctaaaaa
       61 ctaaaagtga tagcactttt aattataaga agttagaata ttaatcattt gcttaattgt
      121 acaatataat gtacaattgt tttatagaaa taaataaggg gtgaaaggaa tggaagcagt
      181 agcttattca aatttccgcc aaaatttacg tagttatatg aaacaagtta atgaggatgc
      241 tgaaacactt attgtaacaa gtaaagatgt agaagataca gttgttgtat tatcaaaaag
      301 agattatgat tctatgcaag aaacgttgag aacactttct aataattacg tcatggaaaa
      361 aattcgtcga ggagatgaac aattctccaa aggtgcattt aaaacacatg acttaatcga
      421 ggttgaatct gatgattaag gcttggtctg atgatgcttg ggatgattat ctttattggc
      481 atgagcaagg aaacaaaagc aatataaaaa agattaacaa gttaataaaa gatatcgatc
      541 gttccccctt tgctggatta ggaaaacctg agccattaaa gcatgattta tctggaaaat
      601 ggtccagaag aattacagat gaacatagac tgatatatag agttgaaaat gaaacgatat
      661 ttatttattc tgcaaaagat cactattaac caatcggaag taaggaaagg gtcagaaact
      721 taaaagtttt tgatccttat tttatttacc ctagtcattt aaaaagctaa tatagcttag
      781 tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta cggatataag gttagtaaaa
      841 tcatttctaa agttgaggaa aagtaaatat aaatggctta aatttcaaca atttgaagtt
      901 gaatagatat gttataatac tattgtagtg tgggatgtta gttactaaag gatgacgctt
      961 atatatatga ctgaatagaa taagcaatag gtttaataat ctattttaaa ttttttgtac
     1021 tagttttagt taattagcaa aaacaacaaa aataaacttc tcatagaatt tagctaaaaa
     1081 ttaatgattt atttaca
//
LOCUS       DANJMA010000173         1062 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000173 DANJMA010000000
VERSION     DANJMA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(37..993)
                     /locus_tag="PWU31_002840"
                     /pseudo
     CDS             complement(37..993)
                     /locus_tag="PWU31_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          177 c          159 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcattcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DANJMA010000174         1036 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000174 DANJMA010000000
VERSION     DANJMA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            158..919
                     /locus_tag="PWU31_002841"
     CDS             158..919
                     /locus_tag="PWU31_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL1085289.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          297 a          162 c          198 g          379 t
ORIGIN      
        1 ttgtttcttt tttagaatca aatgtgatta aatagaaaat tttacaataa tatgcacata
       61 taaataataa gatgatataa tcaacaaaga aaatgtgatt tttttcacta tattttatca
      121 catgaaaatt cattaagaaa gtaggaatcg aagtattatg cacacaccag aagaaattat
      181 ggaactcagt attcatcttg gaacaaaaaa aatacataaa cctatcttag ctaaactgat
      241 tttaggtttt atcggaggag ccatgatttc tctaggttat ttggcatacg tacgagtctc
      301 tgcttctatc ccagaatcta tggctagctt gactagttta gttggtgcct cggtttttcc
      361 tatcggcctg ctggtcatcc ttcttggcgg tggtgaattg atcaccggaa atatgatggc
      421 tgtggcgatt gcctttatgg ataaaaaagt cacattctca cagctggtta agaattggtc
      481 agtgattact tttgcaaata ttattggtgc aattttcgtt gcctatttct ttggacactt
      541 tgttggttta actcattcag gtgtctatct cgaccaagtg attcatattg ctgatcataa
      601 aatcgctgtt tcttggtggc aagcattagg ctccggaata ggttgtaatt ggtttgtcgg
      661 tttagcgtta tggttatcat atggagcaaa agatgcggcc ggcaaagtac taacgatttg
      721 gtttccagtc atgatttttg tggcaatcgg tttccagcat agtgtggcca actgttttgt
      781 gatacctgct gccatatttg aaggtcaagc gacttggtta gaatttctct cgaattttat
      841 ccccgtttat attggaaata tcatcggcgg agctattttt gtttcaggtt tttattacgt
      901 tagttataag catcattaag attgaaggta atgttcattc gagcattacc ttcaatctct
      961 ttatatacta tttatatttt aagaacaata gccttttatt atatatatta ttcaaattag
     1021 ttatatcgag tgaaaa
//
LOCUS       DANJMA010000175          994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000175 DANJMA010000000
VERSION     DANJMA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..>994)
                     /locus_tag="PWU31_002842"
     rRNA            complement(<1..>994)
                     /locus_tag="PWU31_002842"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DANJMA010000176          981 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000176 DANJMA010000000
VERSION     DANJMA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..566
                     /locus_tag="PWU31_002843"
     CDS             <1..566
                     /locus_tag="PWU31_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085290.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          339 a          167 c          211 g          264 t
ORIGIN      
        1 aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa ggtcacagtc agccataatc
       61 gtttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacggag cttctacgac
      121 tagaaacaga aaaatacatc atgaatgttt acctagaaag gagtgagctg gtcgaaaact
      181 ttgtcagcta cacgatttgc aggaaacact attactcaga atcagaatat tattcaattt
      241 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa gatgcgacga
      301 tagacgatta ttaccttgta tttactagat ttttgacgtg tggaacggaa tctatcgtgt
      361 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
      421 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctata
      481 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
      541 gatttgaaag gaaaaaaagg caataatcat ttataccgaa aagtgagaat gtgtgaaata
      601 aggacaaaaa acctagtaac taagggaatg gttcttattt cgcaccaatc gctccaaccg
      661 cccccgccgc ttaggtggtg cgaaataagc gggcaacacc cgataaccta agagaaagaa
      721 ggagatccat atgaataaaa tcattcctct attgttagtc ctatttgttg gcgaaatact
      781 tatgcgccat gctgcagctg tgcatttaaa cttcgtagta tttttggcaa tactggtatt
      841 ttgtttgatc ttttggcgct ttgtattagc ccttgtgagt ctactattca agatgattgc
      901 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
      961 aggaaataaa cggttacccg t
//
LOCUS       DANJMA010000177          960 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000177 DANJMA010000000
VERSION     DANJMA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>960
                     /locus_tag="PWU31_002844"
     rRNA            <1..>960
                     /locus_tag="PWU31_002844"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          267 a          215 c          289 g          189 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
//
LOCUS       DANJMA010000178          914 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000178 DANJMA010000000
VERSION     DANJMA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..538
                     /locus_tag="PWU31_002845"
     CDS             <1..538
                     /locus_tag="PWU31_002845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085291.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            565..>914
                     /locus_tag="PWU31_002846"
     CDS             565..>914
                     /locus_tag="PWU31_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085292.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSN"
BASE COUNT          381 a          138 c          211 g          184 t
ORIGIN      
        1 ggtcatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccagag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agagcgaatc gaaaataaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg aagtacaagc
      481 aatcattgac agcggacgag tgacgcaatg gaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atca
//
LOCUS       DANJMA010000179          912 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000179 DANJMA010000000
VERSION     DANJMA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            2..>912
                     /locus_tag="PWU31_002847"
     CDS             2..>912
                     /locus_tag="PWU31_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085293.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSN"
BASE COUNT          383 a          137 c          210 g          182 t
ORIGIN      
        1 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
       61 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      121 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      181 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      241 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggact
      301 ggatatgacg atcgataata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      361 tgaatcgact ttttcagccg ccacgatccg aaaatatcaa cggaagttgg aaacattaga
      421 acagctgaaa gaaaaatcag aaaaaggaaa agaaaatatc ctccctgaag tacaagcgat
      481 cattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaagg
      541 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
      601 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcacc
      661 aactgattct ctgaaggaga aagaaaaaga tcatttctat cgagtagaat tcaacgaaaa
      721 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga
      781 tcaacttaaa acgttggata accaatacgc tgcagaagca ggttattata aattctattt
      841 tgaagaagtg aagaacggag aagtagtaga tcagatccga atggatctag gggatggcgt
      901 caacagtaat ca
//
LOCUS       DANJMA010000180          871 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000180 DANJMA010000000
VERSION     DANJMA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(80..>871)
                     /locus_tag="PWU31_002848"
     CDS             complement(80..>871)
                     /locus_tag="PWU31_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085294.1"
                     /translation="YSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNL
                     VFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKF
                     TSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAY
                     ETPEYYFADYGEAVTGDDGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDID
                     HFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          265 a          196 c          128 g          282 t
ORIGIN      
        1 gtgtagttta attctttgct gaaatctaaa gccaaattat ataaactgct agccatttat
       61 atacctcctt gtcttcgttt taaaaacgtt tttggtcaag cactgtgcta tcatatgctg
      121 tatcctcttt taatctaata tcttcatacc ctagacggtg tgccactaaa ttccatctaa
      181 ctaatacgtt tggcttacta gtttcaatga tgaaatggtc aatatcttca tgagtaacag
      241 cacacaaaac tagttctgta ggtgtcacat gtgtcatata ccgacttaga tttactgtct
      301 cagcaaacat ggggtcaata tcaacacgaa ctttaccatc gtcacctgta acggcttccc
      361 cataatcagc gaaataatat tctggagttt cataagcgtt caatagtcgt tgtccataat
      421 gttctgttgg tacagttgag tttttagtac ctctaacagt aaaatcttta tatacttgta
      481 ccgttgattg ttcaaaccta gcaagtttcc catcttccca tgaaccaaaa aaacaacctg
      541 gtaacgttag cataccatca ctagtaaatt tcatagtcct accagctacc ttaaattccc
      601 atgagttacc cgcactacca ttaatgctta aagaactacc gtcgccagaa gttacataac
      661 tagcattgct atacctgaaa ttgggcgcac caaaagatag aaacggtctg ttattaccat
      721 tatcccacgt actaaaaacc aagttaccct gtggatttct aatcatgaaa ccaccaccag
      781 ttttcatggt gtatgataca ataccggcat cagcacttac ataatcacgt gcttctagct
      841 ccataatatc tttgttaact ttttttgagt a
//
LOCUS       DANJMA010000181          839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000181 DANJMA010000000
VERSION     DANJMA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            115..309
                     /locus_tag="PWU31_002849"
     CDS             115..309
                     /locus_tag="PWU31_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085295.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            578..>839
                     /locus_tag="PWU31_002850"
     CDS             578..>839
                     /locus_tag="PWU31_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085296.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQK"
BASE COUNT          351 a          106 c          134 g          248 t
ORIGIN      
        1 aaaaaaactt tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt
       61 tcacttacat atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat
      121 tatgtagaag taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac
      181 gaatatcctt tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata
      241 aaaataaaga acgagctaga aaaagaactg acttgtggca aagctgttgt agatatcatc
      301 gaaaagtaaa tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc
      361 taagattata gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt
      421 tgtataccat atgattattt caatatgtat cctggaattg gagatctcgt agagctatat
      481 caagatgaat caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt
      541 atttttataa tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga
      601 cagtgaacta cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc
      661 ccacaaagtg acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg
      721 ttcggcaaaa ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc
      781 atttgcttta aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaa
//
LOCUS       DANJMA010000182          836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000182 DANJMA010000000
VERSION     DANJMA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..>836)
                     /locus_tag="PWU31_002851"
     CDS             complement(<1..>836)
                     /locus_tag="PWU31_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1085297.1"
                     /translation="DAQSVLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVS
                     FVDFKDKATDVATLREAAKDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVS
                     MGDGVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLIND
                     SIDQLKNGGEISDLINDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARN
                     VLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYR
                     TS"
BASE COUNT          253 a          181 c          114 g          288 t
ORIGIN      
        1 atacttgttc tataatcagc accatcacta attaatgtag tacctctaat agtaatccct
       61 actatctcac cagctgtaat aaacgaggca ttgaatccgc catctaacgt ccatgcggtt
      121 tcatatgttc cattaatacc agttttagaa aaaccaatac cagcgttgtt gatttgtaaa
      181 acattccgtg cggtattctt atctggcgtg tccataatca aaatacgact aggcgcttct
      241 ttaggatcta ataaaacata accaccattt tgaccagtaa tcatatcagt ttgatgatct
      301 acaatatcat tgattaaatc actgatttcg ccaccatttt tcaattgatc aatggaatca
      361 ttaatcaaat tgcttacatt attctctgtg ttttctaaga agtttgtttt gacgtttcct
      421 acaactaatt tatcgtatga attggttaga acattaaacg tatattccac aattctcgct
      481 gacatattca cttttaactg tggatgatac acatctactc cgtcacccat cgaaactttt
      541 tctagatcaa caaatttttc atagcctctt tgatgcctca atggtactaa ttcaatcgaa
      601 ccactcactt gtggtttttg tttatctatg tttgttttca accagtcttt agcagcttcc
      661 cttaatgtgg ctacatcagt cgctttgtct ttaaaatcaa caaaagaaac atatccagca
      721 ggataatcat ccacgtaatc cgtgaaaata acttcttctg gtagagtgat ctcgtcttct
      781 ccttctgaag aactgctaat gaatggataa actccaacta aaacactttg agcatc
//
LOCUS       DANJMA010000183          836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000183 DANJMA010000000
VERSION     DANJMA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..>836)
                     /locus_tag="PWU31_002852"
     CDS             complement(<1..>836)
                     /locus_tag="PWU31_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1085298.1"
                     /translation="DAQSVLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVS
                     FVDFKDKATDVATLREVAKDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVS
                     MGDGVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLIND
                     AIDQLKNVGEISDLINDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARN
                     VLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYR
                     TS"
BASE COUNT          256 a          179 c          111 g          290 t
ORIGIN      
        1 atacttgttc tataatcagc accatcacta attaatgtag tacctctaat agtaattcct
       61 actatttcac cagccgtaat aaacgaggca ttgaatccgc catctaacgt ccatgccgtt
      121 tcatatgttc cattaatgcc agttttagaa aaaccaatac cagcattgtt gatttgtaaa
      181 acattccttg cggtattctt atctggtgtg tccataatca aaatacgact aggcgcttct
      241 ttaggatcta ataaaacata accaccattt tgaccagtaa tcatatcagt ttgatgatct
      301 acaatatcat tgattaaatc actgatttcg ccaacatttt tcaattgatc aatggcatca
      361 ttaatcaaat tgctgacatt attctctgta ttttctaaga agtttgtttt gacgtttcct
      421 acaactaatt tatcgtatga attagttaaa acattaaacg tatattccac gattctcgct
      481 gacatattca cttttaactg tggatgatac acatctactc cgtcacccat cgaaactttt
      541 tctagatcaa caaatttttc atagcctctt tgatgcctca atggtactaa ttcaatagaa
      601 ccactcactt gtggtttttg tttatctatg tttgttttca accagtcttt agcaacttcc
      661 cttaatgtgg ctacatcagt cgctttgtct ttaaaatcaa caaaagaaac atatccagca
      721 ggataatcat ccacgtaatc cgtgaaaata acttcttctg gtagagtgat ctcgtcttct
      781 ccttctgaag aactgctaat gaatggataa actccaacta aaacgctttg agcatc
//
LOCUS       DANJMA010000184          783 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000184 DANJMA010000000
VERSION     DANJMA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..672
                     /locus_tag="PWU31_002853"
     CDS             <1..672
                     /locus_tag="PWU31_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085299.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          290 a          120 c          146 g          227 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 cca
//
LOCUS       DANJMA010000185          780 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000185 DANJMA010000000
VERSION     DANJMA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>780
                     /locus_tag="PWU31_002854"
     CDS             <1..>780
                     /locus_tag="PWU31_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1085300.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          277 a          152 c          140 g          211 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
//
LOCUS       DANJMA010000186          777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000186 DANJMA010000000
VERSION     DANJMA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(250..729)
                     /locus_tag="PWU31_002855"
     CDS             complement(250..729)
                     /locus_tag="PWU31_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085301.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          262 a          107 c           87 g          321 t
ORIGIN      
        1 ttgacgctcc cttaacaata aatgaagact tttttattgt taattgttta tttttctatt
       61 attagatatg aaaatcgcat ttagataaaa tagaaaaata tgaatatatt ttaaatatta
      121 agatgagagt cattaactta tgatagtagt caatttgctt ttcactaatt acaaaatatg
      181 tgatatagga agtttagtta ttgttctcat aatgccatcc tgtttttaga ttatctagta
      241 atttttttgt tatttaaaat taggagcagt gccaattgtt ttaattttaa aggctttttt
      301 tatatcttct aaattagaag atatttgtat atcaatatgg aatatattac cttttttgta
      361 tttttttatg ttatctttag ctaaaacatc aaatacatgt ccttgaaatt gtatcgaggc
      421 agctggattt agtttaaatt gaggaaactt taccacagct gattcaaaac acttgtcaca
      481 tttttttatt tttttacctt tgtaaaaata ttcgattgaa tattctattt ggcacgactc
      541 acttggcagt atatgtatgt caatattttt catctgtttc ataggtattt caaaatctaa
      601 actattaaaa acatataatt ccctgtaatt atttacatct aactctacta aatttatctt
      661 tggaacaata gatcctccac ccattccacc ataataaaaa aaatcttgtt ctctatcaaa
      721 atagcacata ttaagctcag tttgtttatc atcctcaata attttataaa tcaaacc
//
LOCUS       DANJMA010000187          739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000187 DANJMA010000000
VERSION     DANJMA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            73..690
                     /locus_tag="PWU31_002856"
     CDS             73..690
                     /locus_tag="PWU31_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085302.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          268 a           99 c          142 g          230 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagcaat ggagaaaat
//
LOCUS       DANJMA010000188          726 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000188 DANJMA010000000
VERSION     DANJMA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            53..>726
                     /locus_tag="PWU31_002857"
     CDS             53..>726
                     /locus_tag="PWU31_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDL1085303.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKG"
BASE COUNT          236 a          128 c          150 g          212 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc tggttcttag gctactcctt
      361 cgaggagaaa atcccccatt tctcaacttt tggaaagaat tatgttcgcc gatttagaga
      421 aacgacggtg tttgaggata tttttgccta tattttagaa caagcagtta aagcaggttt
      481 tgtaacagag gataatttgt atctggattc tacccacatc aaagctaacg ccaataaaca
      541 taagtttacg aaagaaatga cccatgatga agcgaaagcc tatcaggatg aattagaaga
      601 tgagatcaat cgtcaacgaa tagaggctgg aaaacgccct tttactttgg acttagaaaa
      661 ggaagtgaag ctgaaagaaa gaaaaataag taaagctgat ccggaaagcg gctactacgt
      721 caaagg
//
LOCUS       DANJMA010000189          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000189 DANJMA010000000
VERSION     DANJMA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(45..476)
                     /locus_tag="PWU31_002858"
     CDS             complement(45..476)
                     /locus_tag="PWU31_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL1085304.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          247 a           94 c           81 g          290 t
ORIGIN      
        1 gtacaattag tattatctgc actactttct aattaaataa atttttagtt attaacttga
       61 ttaaaatcag agataatatc atttaattca gtttgaaggg ttgtaacgat taaatggaaa
      121 ttttcttcat ctgttttatt atatggggat gttttcgtta tatataacaa cttttctttt
      181 tcaattttca ttaataactc tgtcgaagtc tgtactttat cttgataatt aaatgcagac
      241 atcccacttt gtataattgt aattattgct gaagcaattg aaacaatcaa tagtaaagac
      301 ctgtgttcgg aagcctgatc aattaatatt gggatactag ctgataagac tatttttata
      361 atatttccga taccaattac tctattataa aatcgaattt ctttttttaa tctatcgatt
      421 gtagaatcaa tagattcgat aaacgctttt tgatccatca ttttctctcc aatcatttta
      481 ttaaaatcat taacaaaaat attataatag gatttaagta agttctaact aatcacatat
      541 aaaaataaaa aaacatttgt attttatcaa agttttatta tacttgtcaa tttgtgtaca
      601 tcctaaaata taagctattt gtttacttct gtagaatata tactttgttg ttagataagt
      661 tgcacatgtt tatagaatct aaataataaa tttttatgta ccgcccctca at
//
LOCUS       DANJMA010000190          696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000190 DANJMA010000000
VERSION     DANJMA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(53..628)
                     /locus_tag="PWU31_002859"
     CDS             complement(53..628)
                     /locus_tag="PWU31_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1085305.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DANJMA010000191          693 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000191 DANJMA010000000
VERSION     DANJMA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            230..628
                     /gene="spxA"
                     /locus_tag="PWU31_002860"
     CDS             230..628
                     /gene="spxA"
                     /locus_tag="PWU31_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL1085306.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          246 a          100 c          132 g          215 t
ORIGIN      
        1 tttagaaaat ttgaattaga tttgaaaact ctaattttta aatgataaaa aaagcgagaa
       61 tgctggtttt actgtatatt aaccttagat aactttcatt tttttaaaaa aattatgaaa
      121 aaaaggtagc tatttaaaag ttgattatgt ataatagaaa cagatgggag attattatga
      181 tcttctgtta caaaaaagtg attcttatca cgaaaaggag tggtgcacgt tgttaacatt
      241 atatacttcc ccaagttgta cttcctgcag aaaggctcgt gcgtggttgc aagaacatca
      301 gattccattt gttgaacgta atattttttc agaaccattg aacagttctg aattaaaagc
      361 gattttacaa atgacagaag acggaacaga agaaatcatc tcgacacgtt ccaaagtatt
      421 ccaaaaactg aacatggatt tagatgatct gccgttacaa gaattattgg aacttgtgca
      481 aaacaatcct ggattgcttc gccgtccaat catgattgat gataaacgtc tgcaagttgg
      541 ttttaatgaa gatgaaattc gtcggttcct accgcgcgat gtacgacaat tagaattacg
      601 tcaagcacaa ttgatggctg gattatagaa ggataatacg aaacttggaa aagactggct
      661 ctttgtcaaa tgatgttggt aaagagaaat ctt
//
LOCUS       DANJMA010000192          640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000192 DANJMA010000000
VERSION     DANJMA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..283
                     /locus_tag="PWU31_002861"
     CDS             <1..283
                     /locus_tag="PWU31_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1085307.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DANJMA010000193          618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000193 DANJMA010000000
VERSION     DANJMA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>618
                     /locus_tag="PWU31_002862"
     CDS             <1..>618
                     /locus_tag="PWU31_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDL1085308.1"
                     /translation="IKKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNE
                     AKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDA
                     VLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVA
                     ESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT          215 a          103 c          117 g          183 t
ORIGIN      
        1 ataaaaaaag ccttatcaaa acgaggcatt cagatcagcc gacgaaaaat tagtagaatc
       61 atgaaaaata gaggattaaa atcgagctat actgttgctt attttaaagt acatcattct
      121 acttgcaatg aagccaaaac gacaaacgta ttgaatcgta aattcttaag agacaaccca
      181 ttagaagcga tcgtaacaga cttgacttat gtacgagtcg ggaaaaaatg gaattatgtc
      241 tgtttcattt tggatctgtt caatcgagaa attctcggct attcttgtgg agaacataaa
      301 gatgccgttc tagtaaaaaa agcatttagc cgtatcaaac aacctctgac agaggttgag
      361 atttttcata ctgatcgtgg aaaagagttt gataaccaag ctattgatga attattaaca
      421 acttttgaca tcaatcgatc attgagtcat aaaggctgtc cttttgataa tgccgtagct
      481 gaatcaactt ataagtcgtt gaaagtagaa tttgtctatc aatacacatt tgaaacctta
      541 caacaattgg atttggagtt atttgactat gtcaattggt ggaaccacct tcggttgcac
      601 ggtacacttg gctacgag
//
LOCUS       DANJMA010000194          557 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000194 DANJMA010000000
VERSION     DANJMA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>557
                     /locus_tag="PWU31_002863"
     CDS             <1..>557
                     /locus_tag="PWU31_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HDL1085309.1"
                     /translation="ELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHFFRKGITMS
                     EIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMDATYIPLKRQT
                     VSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDVLLFITDGLK
                     GMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          176 a          119 c          111 g          151 t
ORIGIN      
        1 gaacttcatc tccagattcc gcgcgaccgc aacggcgagt tcaagcaaca gactgttcct
       61 gcttatagac ggacgaatga taccttagag gagaccgtca ttcacttctt ccgaaaaggt
      121 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatca ctacacgccc
      181 caaaccatgt ccaacatgac taaagtgctg actgaagaag taaatgcatt taaagttaga
      241 actctaaatg ataagtatgt cgctattttc atggatgcta cttatattcc attaaaacgg
      301 caaaccgtct ccaaagaagc aatctatatt gccattggta tacgagaaga cggcactaaa
      361 gaagtactaa gttatgcgat tgctccgact gaatcgacct acgtttggaa tgagttgtta
      421 caggacattt actccagagg ggttcatgat gtcttactgt ttattaccga tggcttaaaa
      481 ggcatgaaag atacgattca ccagatttat cctaaagcta aataccaaca ttgctgtgtt
      541 cacgtttccc gtaatat
//
LOCUS       DANJMA010000195          553 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000195 DANJMA010000000
VERSION     DANJMA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            143..400
                     /locus_tag="PWU31_002864"
     CDS             143..400
                     /locus_tag="PWU31_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085310.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          234 a           68 c           77 g          174 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt ttt
//
LOCUS       DANJMA010000196          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000196 DANJMA010000000
VERSION     DANJMA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..132)
                     /locus_tag="PWU31_002865"
     CDS             complement(<1..132)
                     /locus_tag="PWU31_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1085311.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DANJMA010000197          503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000197 DANJMA010000000
VERSION     DANJMA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
BASE COUNT          159 a           72 c           88 g          184 t
ORIGIN      
        1 ttttaatttt attacaaatg ttacaaataa atagctatta ttccggagtt tttagattaa
       61 aagtttatga aaacaatcac aaacgtaggt tttaaagtca taatcgttag atattttagt
      121 tatttttcat tcaaaaaaat agggaactag tgtttgcata tgtgcttttt tgagttataa
      181 taattcatga agagaggtaa ttcacttatt tccccaagct gaaaagcctg atattacttc
      241 ttttcaggca gtgctgctaa tattcaatta cacttttttg tatattttta gttgttttaa
      301 atttaattgt ctgatatgct tgtgatgaat tgattttcgt tctgcacttt tggacgataa
      361 attgtgataa ttgaaaatac aaaaaaaaga aaggcccctg taaggctcgg gagactggta
      421 atctccgtca gtgtcctaca ggaaagcatt aagatgacgc cttaatgcgt gcccttcagt
      481 gttacggtat tacccgcaac gga
//
LOCUS       DANJMA010000198          496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000198 DANJMA010000000
VERSION     DANJMA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            1..270
                     /locus_tag="PWU31_002866"
                     /pseudo
     CDS             1..270
                     /locus_tag="PWU31_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          186 a           73 c           87 g          150 t
ORIGIN      
        1 aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata aaccttttcc
       61 ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac gatttatatt
      121 ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt tggtttagct
      181 aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat tgattctcct
      241 caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg atagggtctc
      301 acgctctttg tttttcgcca tttaaagatt gaaatttttg ccatcgccga ttgtaaaatt
      361 aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccga ccaaagaatt
      421 ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt gtaataatag
      481 agtcttattt caaaat
//
LOCUS       DANJMA010000199          488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000199 DANJMA010000000
VERSION     DANJMA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..430)
                     /locus_tag="PWU31_002867"
     CDS             complement(<1..430)
                     /locus_tag="PWU31_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1085312.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          138 a           96 c           66 g          188 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcattgag
//
LOCUS       DANJMA010000200          478 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000200 DANJMA010000000
VERSION     DANJMA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..270
                     /locus_tag="PWU31_002868"
     CDS             <1..270
                     /locus_tag="PWU31_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL1085313.1"
                     /translation="ELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELD
                     RQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            257..475
                     /locus_tag="PWU31_002869"
     CDS             257..475
                     /locus_tag="PWU31_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085314.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
BASE COUNT          181 a           58 c          116 g          123 t
ORIGIN      
        1 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
       61 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      121 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      181 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      241 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      301 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      361 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      421 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgatac
//
LOCUS       DANJMA010000201          476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000201 DANJMA010000000
VERSION     DANJMA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..>476)
                     /locus_tag="PWU31_002870"
     CDS             complement(<1..>476)
                     /locus_tag="PWU31_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085315.1"
                     /translation="EYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCT
                     ISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTK
                     YRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          123 a          106 c           82 g          165 t
ORIGIN      
        1 ccaaactcgt aactcgagct tggaacagct ttttatctac ttcgtttcga atttttgaac
       61 aaaaaaataa aggctccttg cctttccata gccaggtggc taatttcttt aaattcattg
      121 cagcaaacac tagcatcgta tgcgtcgcga ctttttccaa gcctcgatac ttggtccaac
      181 gcatcccatg cttttctttt gcatccgcaa atattcgttc aatcgtttgt ttccgttttt
      241 tgtaaattga tttattcaga tctgttaggc gaagatgttc cacttcatcc atcagatctc
      301 cccaaacatg tctcgtgatg actcgttgat gatttttact tatggtacat tggtttagca
      361 gaggacagtt cacacattct tttggattgg atttgtattc tcggtaccct tctcgagtaa
      421 ccgttgaaaa agcgagcatt tgatcttgcg gacaaatata acaatcaaaa tactcg
//
LOCUS       DANJMA010000202          432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000202 DANJMA010000000
VERSION     DANJMA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..429
                     /locus_tag="PWU31_002871"
     CDS             <1..429
                     /locus_tag="PWU31_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085316.1"
                     /translation="QNPSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNE
                     LLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQK
                     RLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          162 a           69 c           87 g          114 t
ORIGIN      
        1 cagaacccat cactagatcg tttaggaaga gattatgaag atataaaaaa tacagttgct
       61 tttatgaaac agaaaaaagt agcgttaaaa atattagatg cacagttttt agattttaat
      121 actggaaatg aactcctgga taccgctatg ttcgatatgt ttttatcact gctaagttat
      181 attgcacaaa acgagcgtga gaaaattcgt gaacgtcagc ggcaaggagt attattggct
      241 caaaaagccg gccgatataa gggacgacca atcgagtatt ctattaactc aacagatccg
      301 caaaaacgct tagtttttaa aaccgtggtg gacatgttaa agcaaggcat tccagttgca
      361 gagattgcaa aagaaaacgg gatctctcga ccaactattt ataaaataaa gaaagcgaat
      421 gatttgtaga aa
//
LOCUS       DANJMA010000203          431 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000203 DANJMA010000000
VERSION     DANJMA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>431
                     /locus_tag="PWU31_002872"
     CDS             <1..>431
                     /locus_tag="PWU31_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1085317.1"
                     /translation="FAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFV
                     SSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSA
                     AGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          174 a           64 c          100 g           93 t
ORIGIN      
        1 tttgcagtga aagacggcgt aagcttacca gaagataaac ttccagtgtt gaaagcaaaa
       61 gatggttata cagatgcgaa atggccagag gaagcaactc aaccaattac agcagatgat
      121 acagaatttg tatcaagtgc aacaaaactg gatgataaat ctgacgctga caaatataat
      181 cctgaaggtc aaaaagtgac tacagaattg aataaggaac cagacgcatt tgagggaatt
      241 aagaacaaag aagatttacc aaaagatact aagtatactt ggaaagaaaa agtagatgtt
      301 agcgcagctg gaaataaaaa agggactgtt gtagtgacat atccagatgg atcatctgat
      361 gaagttgaag tagatgttac agtgacagac aatcgttctg acgccgataa atatgagcca
      421 acagtagaag g
//
LOCUS       DANJMA010000204          430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000204 DANJMA010000000
VERSION     DANJMA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>430
                     /locus_tag="PWU31_002873"
     CDS             <1..>430
                     /locus_tag="PWU31_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL1085318.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          145 a           81 c           74 g          130 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa
//
LOCUS       DANJMA010000205          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000205 DANJMA010000000
VERSION     DANJMA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..365
                     /locus_tag="PWU31_002874"
     CDS             <1..365
                     /locus_tag="PWU31_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085319.1"
                     /translation="DPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKE
                     LVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAET
                     LDQLLPWNKDVQQHFSRN"
BASE COUNT          135 a           75 c           84 g          121 t
ORIGIN      
        1 tagatcctgt tgcaaaatcc aagttggatg cagccgttca atatgcgtgt aaactacgaa
       61 gtggttttga acctattttt gaagatggtc gtttagaact cacaaataat cgtgcggagc
      121 gaaatatcaa agagctggtg ataggacgga aaaattggtt gcattccaca agtcttgaag
      181 gagcacgaac ttcaggaatc attctgagtg tatataaaac agcagaactt aacggattga
      241 atcctgtaaa gtatctagag tttctatttg ataagatacc aaatcttccc gtactttcag
      301 cagaaacttt agatcaactg ttgccttgga acaaggatgt acaacaacat ttttctcgta
      361 actaaaaacg ccaaacattc ccgattctat tctataggtc gggttatttg acgta
//
LOCUS       DANJMA010000206          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000206 DANJMA010000000
VERSION     DANJMA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..339)
                     /locus_tag="PWU31_002875"
     CDS             complement(<1..339)
                     /locus_tag="PWU31_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1085320.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANJMA010000207          412 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000207 DANJMA010000000
VERSION     DANJMA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..387
                     /locus_tag="PWU31_002876"
     CDS             <1..387
                     /locus_tag="PWU31_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085321.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           74 c           65 g          115 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tt
//
LOCUS       DANJMA010000208          398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000208 DANJMA010000000
VERSION     DANJMA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..>398
                     /locus_tag="PWU31_002877"
     CDS             <1..>398
                     /locus_tag="PWU31_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1085322.1"
                     /translation="SALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVI
                     VGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREK
                     DYSQFSNEFGHLEGDTIVGLKHKSAVITLV"
BASE COUNT          128 a           73 c           84 g          113 t
ORIGIN      
        1 tcagccttag aatattatca acagtataag aaaaacaaat caaactgtgg tagacgtccg
       61 cttgttttac ccgaggaaca atcagaatat attcaaagaa aggttgttca aggatggaca
      121 cccgatgtga ttgttggtcg tgcagcgttt cctattagtt gttctgctcg taccatttat
      181 cgtatgttca aaaaggggct atttgattct tctgacttac cgatgaaagg caagcgtaaa
      241 ccgaatggac atcaagaaag acgtggaaaa caaactttcc gccgctctat tcatgaacgt
      301 gaaaaggatt atagccaatt ctcaaatgag tttggtcacc ttgaaggtga cactatcgta
      361 ggtctgaaac ataaaagtgc tgtaattacc ttagttga
//
LOCUS       DANJMA010000209          371 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000209 DANJMA010000000
VERSION     DANJMA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(<1..347)
                     /locus_tag="PWU31_002878"
     CDS             complement(<1..347)
                     /locus_tag="PWU31_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085323.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMS"
BASE COUNT          105 a           62 c           66 g          138 t
ORIGIN      
        1 tcagacatcg taacaccttt ttcgaataaa tggataatag tggtttccaa tgtatcgttt
       61 gttcttttgt aggctggtaa agtttgttgt ttaaactcac cattacgatc tctaggtatt
      121 tccaatgtta attcaccata ttcggttttg attgatcgaa agtaagaacc gtttctcgaa
      181 ttacctgaat taaaaccagt gcgatcatat ttttcgtaat ctaaaaaagc cgttaattca
      241 gtccgtagga gtgtgtttat cgctttttct aagtgcgaac ggaataattc atttaaatcg
      301 cctttagtga ctagagtttg cacaatttct gtagtaaaat cattcatagg gaagtcctct
      361 tttctgtgaa t
//
LOCUS       DANJMA010000210          313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000210 DANJMA010000000
VERSION     DANJMA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(109..>313)
                     /locus_tag="PWU31_002879"
     CDS             complement(109..>313)
                     /locus_tag="PWU31_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HDL1085324.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           80 a           73 c           67 g           93 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctccgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttg
//
LOCUS       DANJMA010000211        15104 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.055, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000211 DANJMA010000000
VERSION     DANJMA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(102..545)
                     /locus_tag="PWU31_002880"
     CDS             complement(102..545)
                     /locus_tag="PWU31_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL1085325.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(740..2080)
                     /locus_tag="PWU31_002881"
     CDS             complement(740..2080)
                     /locus_tag="PWU31_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1085326.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2362..3258
                     /locus_tag="PWU31_002882"
     CDS             2362..3258
                     /locus_tag="PWU31_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL1085327.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3401..4342)
                     /locus_tag="PWU31_002883"
     CDS             complement(3401..4342)
                     /locus_tag="PWU31_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1085328.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4468..5589
                     /locus_tag="PWU31_002884"
     CDS             4468..5589
                     /locus_tag="PWU31_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1085329.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5847..7349
                     /gene="eat(A)"
                     /locus_tag="PWU31_002885"
     CDS             5847..7349
                     /gene="eat(A)"
                     /locus_tag="PWU31_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL1085330.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7377..7943
                     /locus_tag="PWU31_002886"
     CDS             7377..7943
                     /locus_tag="PWU31_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1085331.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            8155..9216
                     /locus_tag="PWU31_002887"
     CDS             8155..9216
                     /locus_tag="PWU31_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1085332.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9219..10115
                     /gene="murQ"
                     /locus_tag="PWU31_002888"
     CDS             9219..10115
                     /gene="murQ"
                     /locus_tag="PWU31_002888"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1085333.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10129..12111
                     /locus_tag="PWU31_002889"
     CDS             10129..12111
                     /locus_tag="PWU31_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1085334.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12181..12627)
                     /gene="nrdI"
                     /locus_tag="PWU31_002890"
     CDS             complement(12181..12627)
                     /gene="nrdI"
                     /locus_tag="PWU31_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL1085335.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12838..14262
                     /locus_tag="PWU31_002891"
     CDS             12838..14262
                     /locus_tag="PWU31_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1085336.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14439..>15104)
                     /locus_tag="PWU31_002892"
     CDS             complement(14439..>15104)
                     /locus_tag="PWU31_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1085337.1"
                     /translation="KKYTTIIDPVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTL
                     KNLLYRKYSRGEILVSKWITLVIYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQT
                     LIQSLFTYTLGSYIGLWLILSLVLMIACFINSSGASISAGIVFYFASSIISGILIALI
                     QKWEWIKWNPISMLNLQNQIGNEEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKR
                     KNV"
BASE COUNT         4890 a         2729 c         3168 g         4317 t
ORIGIN      
        1 tcacaacctc tgattcacgg tgttccagaa gtagcttctt tttatttctt gaagttgatc
       61 acttctggaa catcctcttt ttttatttgg atggataatg gtcattccat ttctttgatc
      121 atttttttca ttttgtcgat ttcttcttct tgtgcctgaa tgatttcttc cgctaatttt
      181 tttacttcag gatcttttaa atcagctcgt ttacttgtca agatagcaat agagtgatga
      241 gggatcatcg ccttcatcca cgccgtatcg ttgacagttg tttggcttcg taccataaat
      301 aaggaaccgg caaaaacaag aagactagcg gctaaaatca tagcatttat ctttttgttt
      361 tcatacattt tccacatgaa caacaacatg atgactgcca tgactgcacc catcatcaac
      421 gccatgaata cacgtgtgcg actgaagaag atatgatcaa gcgcataaac attcaaatac
      481 atcataccaa acatgactac cgtcgaaaca ccaatcatca acaaaaactt ccaatacttt
      541 ttcatttaaa acaaactcct ttttcttcat tcctttcttc tatcatgtca tgagcgtgga
      601 gaacaatcaa ttaatctgca cagatggttt ttgaacaccg tttattcgga aaaaagaact
      661 gcaacaattt ttgtcgcagc tccttttttt caatagctcc agatattcaa tataaataga
      721 ataaaattct ctattggttt cattcattcg tcaagacgaa agcatcattc tttttttgat
      781 aactgaaata aatgaacagg taaagcaaac cggtcaatac agttacccca atggagttcg
      841 tgaataggat accaaggaaa atcaaccata agactgctaa tgcaaaaaag ctgattcctt
      901 tgtcccataa cgttcctttc cttagtgtca gtacaaagga cgcagcactg ataatgatcc
      961 agatcaataa tactgtatag gaaagagaac ctgccagata gccaaataat ttgtcaccga
     1021 caaaataaga aagagccaca cctagataaa gaacactggt acaaaacagc acagcacgtt
     1081 gcggtacttg atgcttgttt aaaacagcta gttttctaga ggaacctttc cgatctttca
     1141 agcggaagaa caaaattctc gaagaagcat atacacctga attgatagac gagaacagag
     1201 caagaacaat cacgaaattg acgatatctg ccgcaaatgg aatattcatt ttttcaaata
     1261 ccatcacaaa aggactaacg ttcgtgcctg ctaaagtatt ccaaggaaaa ataatcaaaa
     1321 gcaaaaataa aggaataatg taaaaggaaa tgattcttcc catgacgcct ttgattgctt
     1381 taggaatcgc acgtttagga tcatctgctt cgcttactgt gatagcaatc aactcagaac
     1441 cgccgtaaga atagataact acaagcaaag aactgatgat tcccttcata ccgtggggag
     1501 taaaaccacc atgatcagtt aattgttgaa gggtagggaa aacaccagta cccaacattt
     1561 gctgaccgac aagataagca gcaaaaatga tcaataggat aatgacactg attttagcaa
     1621 aggccagcca atattctgtc tctgcgaata atcgtaccga ataaagattg atgatagtag
     1681 ttaaaacagc caacataaaa acaaataccc aagctgggat gtgggggaac cacaactgta
     1741 aaaaggaagc agctgcaaca gcttcagcaa tgatattgat aatccacatg gaccagtaga
     1801 cccaatcggt aaaatcggca gcatgagcac ctagatatgg acgaatcaat ccagataatc
     1861 cgtggtgcgt atcaccacca cttaaaacaa tttttcccag acctttcatc acgatatata
     1921 aaattatgcc gccaatcaaa aaagcgatca atacagaagg gccggcgatt ccaatcgctt
     1981 cgccacttcc tttaaataga cctgcaccaa tcgctccacc taaagctagc atggtgatat
     2041 gtcgggagct aagttgtttt ttcaattttt tatcttccat aaataacctc tttcttttaa
     2101 attcacaatc aaaatgcata gtaagaataa atagtaagaa taaatagtaa taataataat
     2161 gactaatgat aacaaatttt cagataatta caactattca gaaaactttt gggcatattt
     2221 taatggaaga atgatttatt tcatcgtatt ttcatagaaa tgttaaattt ttataaaaat
     2281 aataggaagc gttttactgg ctttttacta tttaaacagg ctatcatagg aaaaagtgac
     2341 agagaaagtg aggcggaaca gatgaacaaa aacgacgtat tgctaattgc gaaaaataaa
     2401 tcactttatt tttttccttc catccgctct ttagatgttt ggtccggaaa agatttacta
     2461 cagtcggaat tcattaaatc agctcgacaa cagttggcaa attatcagct gatcgttttt
     2521 ttagattatg gatttgatat ggagatggct cgacgcgtgc gtccatttac caatgcaaaa
     2581 atcgtgttat ttttttggaa ccactttaaa gcagaacacg aggcgatgct gaagcaggca
     2641 caagatgaac cggcaatcga tgagatttat cattttgatt ttttagaagc gaaagaactt
     2701 ggactaaaac ataatagttc cttttattcg aaacatatgg atttttcttc tagcgaacca
     2761 aagacggatc tattttttgg tgcaacaaat aacggcagaa aagaacgggc agaaaaatat
     2821 aagcaggaat ttacaaaaag aggtgttacg accaattact ttattcttcc gcttcgagga
     2881 aatgaacaac ctggatacct tacttatgaa gaatatctga aaaaaaccgg ggaatcgagg
     2941 gggattctag aactgttgcg ggaagggcag caaggagtga ctttgcgaac gtttgaatct
     3001 atgtattttc aaaagaaact agtgacagat aatcaagcca tagctcatta tcagttttat
     3061 cattctgaaa atattttttt gcttcaagaa cgtgatttag atgaattacc ggaatttttg
     3121 catacgccat ttcaaccaat cccatcagaa ctgttggaat tttttgatgc agaaaattgg
     3181 gcaaaacgtt ttttcactac tgactccgct atttatgaac aatatgaata cgatctttcc
     3241 tctgttaaaa ataaataaaa gtgccaacca atcatagatg aaaataaaca agcgcaattc
     3301 ttatggcaac atgagaattg tgtttttctt tttgaacttt catgggggta aaaaaatgag
     3361 aaccagctgc tgatccagct gattctcatc agagagcctt ttattctgcg ccaaggattc
     3421 cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt
     3481 tttgtccttt tcgccaaacg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt
     3541 tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag
     3601 aagcaatatg ttggtacact tcttcttgat ctaatgtgat tccttctttt gctgcgatag
     3661 aagcaaactc agatacaatc gttcgaacca tgccctctgc cgctttttgc gcacctaatt
     3721 cagcaatatt acaatccaat atcgtgcata atccatttaa tgttccgttt acacaagctt
     3781 ttcgccaaat ggaataacga acattatgac tatagattgg attcaatttt gcttcttgaa
     3841 agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga
     3901 tttctccatc acctaataat gtgacttttc cgggaccagc tagaccagct gtccacatcg
     3961 tgatcccaaa taagatattt ttctttggta catatttttc cagtacatct tcatgaccta
     4021 atccattcag taaacacaaa acataagtat tctcagtaat aatggattgg atcgaacgaa
     4081 acatatcatc taattgattc gcttttgtca aggctacgat caaatccacg tgtttgttgc
     4141 tttcgatgat ttcttctggt gaatagattg gcagttttgc aactacttct ttgccattaa
     4201 agtcagcaat caatccattt gtacgaatgg cttcaatgtg agcaggccag cgatcgatca
     4261 agacgacctc atttccgctt tgatgcaaca ttaatccgat tcggctgccc attgctccgg
     4321 ctccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt
     4381 atatagaaaa acagaagaaa atagcattgt tttgtttgaa aaaagtgaaa aaaagtgtct
     4441 tacttaagaa gaaagtgagg gatatagatg gatattttgg cggcaatcga ttcatttaaa
     4501 ggaagtgctt cttcatttga attaaatcag gcagttctaa atggttggga agaccgtaaa
     4561 ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat
     4621 tacgcgctcg ggggaacctg gcgtacgatt gatgggatgg atctcttatt tcgtcagcga
     4681 aaaatcagtt atttattgac gaactggcaa ggaaaaaata ccgcagtcat tgaatctgca
     4741 caagtattag gattagattt acttgataaa ccaacggatc aaaccatccg acttgcttct
     4801 agttatgcct tgggagagct tatcaaagat acactgcttc aagaagtgga acaaatcatt
     4861 gtcactttag gaggaagcgg atgtagcgat ggtggtctag gattactcca atctttgggt
     4921 gcttctttag aagggtacac agaaggaaat cctttgttat ctactagaaa acttcatttg
     4981 gatcgaagga acgttcttgt ttctaaagta aaagtgatca tcgctgcaga cgtgaccaac
     5041 ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaacggat
     5101 acgacgttag ctttcttgga taaacaagct gaaaagattg ctcagcagat aaaagatgtt
     5161 tgggaggtgg atttagatga catcccagga agcggagcag caggcggtat tggcggagcc
     5221 ctttttttgt taggagcgaa gatggtctct ggttttgagt tggtcagcca aatcactgga
     5281 ttagaaaaac agatcgaaca agcggatgtc atcattacag gagaagggcg gattgatcag
     5341 caaacaatcc acggaaaagt tccatatggc gtagcagtgc ttgcaaaaaa atatcgaaaa
     5401 ccaataatcg ctatttgcgg aagtagagac cgagagctgg aaggactctt tgattttctc
     5461 cccattgtct taagcattca attaggacca atcgatttag aggaagcaat ggaacacagg
     5521 caaacattag aaaaaacaga aatcttagga cagacactca gcagattttt caacattctc
     5581 ccgaagtagt tgacaaaaac aatttcttta tgctatcgta atttcaaata taggacaaag
     5641 tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc
     5701 ttgtggagac aagggagcag tgtgcagatt ttcgggattg gcttggttct ttcgatgtat
     5761 gggtcttttt gtgtgcccca acccttgcat cctctttagc cagttctgaa aataggactg
     5821 gtcttttttt acgaggtgat ggaataatgt ctaaaatcga aataaaaaat ctgacattcg
     5881 gctatgacag ccaaggcaca ttattatttg aacaagccaa tctaaacttt gacacacaat
     5941 ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg aatatcctgc
     6001 aaaacaaact cccttatcaa gggcaagtga tccatcagca agcgtttgtc tatttcccgc
     6061 agcaaacaaa agataaagag cgtttgacct attatgtgtt aaatgagatt atggattttg
     6121 agatatggga aattgaaaga gagctccaac tgatgcaaac agatcctgaa atcttatgga
     6181 gagaattcag cacactatca ggaggagaga agacaaaagt cctactggca ctcttatttg
     6241 tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggca
     6301 gaaaacaagt agcggcttac ttgaaaaaga aaaaacaagg attcatcgtg gtgagtcatg
     6361 atcggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaag agccaactgg
     6421 aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg
     6481 aaatagctaa aaatgaaaaa ttgaaaaaag aagtcagcag gctaaagaaa acagcagctg
     6541 aaaaagcaga atggtctcgt tcccgagaag gggacaaaac aaagaaaaaa gtcggattca
     6601 tcgatacgga atctaggcga gtgaataaag gagcagtagg cgctgatgct gcacggacga
     6661 tgaaacgatc taaagcaatc gtgaatcgga tggaaaccca gatcagtgag aaagaaaaat
     6721 tattaaaaga tatcgagtat atcgatgctt tgacgatgaa tagccaagtg tctcatcata
     6781 aacggctgtt aagcgtagaa aatcttcaat tagggtatga aaatctgtta ttcgagccag
     6841 ttcattttac gatcgaacct catcagcgga tggcaatttc aggtcctaac ggcgcgggaa
     6901 agtcatccat tatccattat cttctgggga cattcaacgg caaggttgta ggagaaaaat
     6961 cccagcccaa acatttgacc attagttacg tacgccaaaa ttatgaagac aatcgaggga
     7021 cattggcgga attcgcgaag aaaaaccgag tagactatca agcatttttg aataatctcc
     7081 gaaagcttgg gatggaaaga gatgtttttc ataataaaat cgaacagatg agtatgggac
     7141 aacggaaaaa agtggaattg gctaaatctt tatcccaacc agctgagctc tatatatggg
     7201 atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa ctgattttga
     7261 acgtgaaacc tgctatgtta ctagtggaac atgatcaaac ctttctagat aaagtatcta
     7321 ctgagattat ttctcttgag aaaatctaaa ccttgtaatc tcaaagacaa caaggtatgg
     7381 ttaaggagaa ctttgtcaac gaaggagaga atcacatgct ttatgtaacg agacacggag
     7441 aaacaacttg gaacgcacaa ggattagttt gcgggcgagc agatgtgccg ctgactgaaa
     7501 aaggacagat gcaggcacag gcactagcag aaaaggtagt agatttgcct gttccaatca
     7561 cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca gtagttgatc
     7621 gtctcaattt gccgatgacc gctgatgagc gattggtaga aatggatttt ggggattatg
     7681 acggactgcc aagcaaagat gaaaactttc aaaaagcgcg tttagcgttt gctgtacgtt
     7741 tcccaaatgg agagtcggtc ttagatgtgt acgcacggat cgttccttta ttgaaagaat
     7801 gcatggaaga cgaagaaaat gtctacttgt tagtctgtca taatgccttg ataagagtga
     7861 tcaatgctta ttttcatcca atgcctaatg aagggttctt tacattcatg gtagacaata
     7921 cagaattaat ttcttatgag taattagact tttgtgttat ttaaataaaa tgtttcaagg
     7981 cactgctttt gcttagcagt gccttttttg tggctgtgtc aattcttttg gcattataaa
     8041 tccaattttt agaaataaaa tttaattatt ttaacaaaag tattgaaatt ttgtttcttt
     8101 agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga taatatgttt
     8161 ggcttttcaa tatttatgaa tgattctttg tcagaagaga agaaacagta tatccgccaa
     8221 atggcatcca atggctttgt cggtattttc acttccatgc atataccgga agatgatgtt
     8281 tcggcttatc aaaatcgatt gatcgaacta ggaagcgaag cgaaaaagaa taaactagag
     8341 ttgatggtcg atatttcagg agaggcattg aaacgagctg gcttttcatt agaagatcta
     8401 acacccttga aagcaattgg tgtaacaggg ttaagaatgg attatcatat ttctaatcag
     8461 cagatcgccg actggtcaca tcaattgaaa atcagtttga atgccagcac gatcacgcct
     8521 aatgatatcg atgagttgaa agaagcagcg gctgatttct cacagattga agcttggcat
     8581 aattattatc cacgtcttga aacagggttg gacaaagaat ggtatcagga aaaaaatcaa
     8641 tggctcaaaa ctcaaggatt ccttgtacaa ggctttgtac caggagatac agagttgaga
     8701 ggtccactgt atcaaggatt accaactttg gaagaacatc gagggatcca tcctctggca
     8761 ggtgctcttg atctattagc aagtaacacg gatattgttt atatcggaga tgcaggtctg
     8821 tcagaacacg ttcaggagca atttgcttct ttccaaaaag aacagacagt cttgctccat
     8881 acggaacctg tagatgaagt tttttacgaa tatatattag gaaaacatac gaatcggcaa
     8941 gatgatgcaa gagatgtcat acgaagcgca gatgcgcgtt ttcgagagat tccgccaatc
     9001 ccagtaagaa atacagctaa gcgtatgaaa ggatctatta ctttagataa cgaaaagtat
     9061 ttgcgctata tgggtgagat ccaattaact aaatatgatt tgccagcgga tgaaaaagtg
     9121 aatgtagtag ccaaagtcat caaagaagaa ctaccgctga tcaaccaaat caaagctggt
     9181 atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc aatttagaga
     9241 acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgaaatg agtatttctc
     9301 aagcagtcca actcatgaac aaagaagatc agaaagtggc cgctgctgtt gaaaaagaac
     9361 tgccaagtat cgagccagtg atcgccaaca cgattgaatc ctttaaaaaa ggtggtcgct
     9421 tgatttatat gggagctgga acgagtggta gacttggtgt attagatgct gctgaatgtg
     9481 taccgacatt cggtgtagaa ccggaaatgg tcataggcct aattgctggt ggagaagaag
     9541 caatgactgt agccgtcgaa ggagccgaag attcttatga attagggcaa gaagatctaa
     9601 tcaagctgaa tttaacaaaa aatgatatag ttattggtat cgcagctagc ggtcgtacac
     9661 catatgtgat tggtggatta gattacgcaa aatcgattgg tgctgttaca ggaacgattt
     9721 cttgcaataa acaagcagaa atcagcgagc atgcggattt cccaatcgaa gtcgatgcag
     9781 gaccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa aaactgattt
     9841 tgaatatgat ttctactatc agtatgatcg gtatcggtaa agtattcaat aacttgatgg
     9901 tcgacgtcaa accaacaaat gaaaaactaa ttgaacgctc aaaacgaatc atcatgcaag
     9961 cgacagaagc tgactacgaa acagctgaaa agtactttga ggaagcagat caaaatgtca
    10021 aactggctat cgtcatgatt ttgacaaata gtgataaaga ggcagctcaa gcaaaattag
    10081 ctgaagcaaa cggttttgta aaaggaacct tatgaggggg aaaagaccat ggcaaaaaac
    10141 gaattgactt tggaagaact ggcaaaacaa atttatcaag gagcaggcgg tatgggcaat
    10201 gttgaaagca ttgtccactg catgactcgt gtacgtatga aggtcatcaa tgatgatctt
    10261 gtaaatgtct cggaattaaa agcgattcca ggagtacttg gcgtagtaga ggatgaacag
    10321 ctccaagtca tcatcggacc tgggaaagtg aacaaagtag cgcaggaaat ggttgactta
    10381 gcaggcgtga aattaggaga aaaacttcct tctgctagtg caactacagc aagttcttct
    10441 gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg
    10501 aaacaaaaac cctctaaaat caaatcggta ttaaaagaca tctctaatat ctttgtacca
    10561 atgatccctg catttgtcgg cactgggatc gtggcaggga ttgccgcagt tttagcaaac
    10621 ttagtcacag ctggaacact agacgcagga acatggcaac aatatatcga tgtgatgaac
    10681 atcttgaaaa acgggatgtt tagctatcta gttatctata caggtatcaa tgctgcacaa
    10741 gtttttggtg caacgccaac gcttggtgga gttatcgggg ctgttgtgat gctgacaggg
    10801 atgaatccag aagcaccact gaaaaacttg tttaccggtg aagctcttgc aactggacaa
    10861 ggcgggatct taggtgttat cttcgcagtt tggttattgt caatcgttga gaaaaaactg
    10921 cataaagtag tacctgatgc ggtagatatc atcgttacgc caactctttc cctgattgcc
    10981 atcggtctga tcgaaatttt cttcatcatg cctttagcag ggttcatttc tgacggcatg
    11041 gtcggtggga tcaactgggt acttggtgtt ggaggagcat ttgccggctt cgttctaggt
    11101 acattattct tgccaatggt catgttcgga ctacatcaga tcttgacgcc aatccatgta
    11161 caaatgattg atgaaactgg tcgtacctta ttgcttccaa tccttgcgat ggccggagca
    11221 ggacaagtgg gtgcagcatt ggcattatgg atccgctgca aaaaagacaa agaattaaca
    11281 gaaatgatca aaggggcttt accagtcgga atcttaggaa tcggcgaacc attgatttac
    11341 ggagtaacat tacctctagg acgtccattt attactgctt gtatcggtgg aggtatcggt
    11401 ggggcagtga tcggaatgat cggaaatgtc ggagcaatcg ctattggacc ttcaggagcc
    11461 gctttgattc cattgatctc tgatggtaaa tggttaggct atatccttgg tttattggct
    11521 gcttacgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga ccgtttagaa
    11581 aaagaagaac tagcgaccga aacagccaca gtaaatgaag tacctgaaaa agcagtacct
    11641 caagcttcag ctgaaagtgt gactatctta tcatcagttg ctgacggaac agttgtttct
    11701 ttagataaag caagtgaccc agtttttgca caaaaaatga tgggtgaagg ttactttgta
    11761 gaaccagaaa acggacatat ctattccccg gttactggag taataagttc tgttttccca
    11821 acaaaacatg ctatcggcat tacgacacca aatggcttag aaatcttgct tcacatgggc
    11881 atcaacacag ttgacttagg cggtacgcca ttcgatgtga aagtaacaga aggacagcaa
    11941 gtgacacctg atacgcttgt agcagaagct gacttagaag caatcaaagc agcagacaaa
    12001 gaaacctcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa
    12061 aaaacaggta aagcgaaagc aaaagcacct gtgatggatg tcaaagctta ataagaatag
    12121 aaacaataga gaacataaaa agaagaagcc gaaatttatt tttcagcttc ttcttttgtg
    12181 ttatttattt ttttctgcca aaatttgata aacgcgtgtc acatcagatg gtgttccccg
    12241 taattcataa tctgccaaga aaggaaagcc gaatctttgt gcgtattgtt tggctgtcag
    12301 acaatattga ttgttgaagt ttttattacc gctaccgact acaccaaggc aaagtttatg
    12361 attgtcttcg tattccagat attcgcgcat cgtttcggtc aagatttctg tgtcaccgtt
    12421 gtctaaccca ttcccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc
    12481 tttcgtaaaa tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc
    12541 ttctgcatgc attttttcag catattctgc tagatgtttg gcaaaagcac gcgtatttcc
    12601 tgaaatcgat atatataaaa tgttcatgga tcatccccca attctttccc tttagtatag
    12661 catcttttta agtttttttg gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat
    12721 aatgagaaaa aagtcggtag aattatattt ctattaaagt ttttattaat taagtaaaaa
    12781 taaaaaaggt ttgttatact ttttgataaa agcggtttta ctttggagga tgcttacatg
    12841 gatacagagc aacagaaaaa aggaattggt ttgattccac ttgctgcctt agtgattggt
    12901 tcagcgattg gaggtggcgt atttggtatt atgacggaca tctccggatc ggctggtggc
    12961 ccagctatct tcagctgggt actcgtagga acagcaatgt tgatgctctc cttatcgatc
    13021 aataatatca ataaaaaacg tccagattta aaaggcggga tctttagtta cgcagaagaa
    13081 ggttttggcc gtttcgctgg ttttatcagc ggttggggtt actggctcac atgctggtta
    13141 ggaaacgtgg cgttcgcaac attgttgatg agtgcattag gttatttctt ccccgtattc
    13201 gaaggtggtc aaaatgtggc atcgatcgtg atgagtacga tcttcttatg gggatatgca
    13261 tggctagtga atcgtggagt tgaaaatgct agctttgtca atgctatcgt cacattttgt
    13321 aaattagtgc cattattttt atttattgta gtagctatca gcaccttttc gatccaacaa
    13381 ttcattaata acttttctag cgatatcata ttgaatgcga atgggcagcc gatcccgttt
    13441 tctagtcaag tattgagttg catcatggtg atgctctggg tattcgttgg tattgaagga
    13501 gcttctgtcg taggttcacg agcaaaaaaa caatcggatg ttgggaaagc aacgattcta
    13561 ggaatattcg gtttgatcat tatttacgtc ttggtttcca tgcttccata tggcacaatg
    13621 acgatgaatc agctgcaaac gatcaaagcg caacctgcta tgggatatgt atttgaaatg
    13681 atggttggta aatggggggc ggtcgtgatc aacggcggtt tgatcatttc tttagttggg
    13741 gtttggctct catggacaat cttgccgatt gaaacaatgg ggaatatggc tgaggacgga
    13801 cttttgccta aagcatgggg aaaactcaac aaaaatgggg caccaactta tgctatcgta
    13861 ttgacgacgt tatgtacgaa tgtcttttta ctttcattat tattcactga cagtgcatat
    13921 aactttgctt atacacttgg tacagcagcg attttcttca cgtggctttt cttagggctg
    13981 taccaaatga agctttctta tcagcggaaa gagtgggtcc aatttttagt agggttttta
    14041 gcaagtgctt ttcaagtatg ggccatgcta gttgtagcct ttaatgaagt gatgcttgtt
    14101 cttgttcttt tcttaccagg tatttatttt tacctcaaag cacgtaaaga acaaaacgaa
    14161 cctgtacaac atatccactt agataagatt ttacttatcg gtgtgacgat ggtcggagta
    14221 atcgcaatgg ttttatttgc cactgggatg cttcacgtat agaagtataa agagaggctg
    14281 tgaagcctgt ggtttattca aaaaagaagc ggtgacaaca ttcgtgtcat cgcttctttt
    14341 tttgaataaa tggtgttcaa gaagtagctt cttctaaaaa taagccgaaa tttcttgaaa
    14401 ctggccactt ctgtcacaac ttctttgtta atgaaccatc atacattttt tcgcttgaat
    14461 acgaggtatc ccaatgctaa gaacaaagca atgtaagcta aattgccaaa cagcatttga
    14521 ttagtagaaa gatgagtcaa ttgcttcata atctcttcgt tgccaatttg gttttgcaga
    14581 ttcagcatac tgattggatt ccatttgatc cactcccatt tttgaatgag tgcgatgagg
    14641 ataccagata tgattgatga agcaaaataa aagacgatcc ctgctgagat ggaggcaccg
    14701 gaactattga taaaacaagc aatcatcaac actaaactta aaattagcca taaaccgata
    14761 taactaccca aagtataagt gaataagctt tggatcagtg tctgaccagt agaaatcttc
    14821 tccgtaaagg aaatcgaagg gaaaaggatc agtttcatca gcacggtgac aataatagct
    14881 aaaaggtata aataaataga ataaataact aatgtgatcc atttacttac taatatttct
    14941 cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtga ttccattgaa
    15001 atgatcgtac ttgcagcagc aatcatgata aagacaatcc aagatgtggc actgaaaagc
    15061 tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt tttt
//
LOCUS       DANJMA010000212        13213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.062, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000212 DANJMA010000000
VERSION     DANJMA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(204..2162)
                     /locus_tag="PWU31_002893"
     CDS             complement(204..2162)
                     /locus_tag="PWU31_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1085338.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="PWU31_002894"
     CDS             complement(2159..3031)
                     /locus_tag="PWU31_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1085339.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="PWU31_002895"
     CDS             complement(3373..4245)
                     /locus_tag="PWU31_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1085340.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="PWU31_002896"
     CDS             4373..4942
                     /locus_tag="PWU31_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL1085341.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="PWU31_002897"
     CDS             5009..5746
                     /locus_tag="PWU31_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1085342.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="PWU31_002898"
     CDS             5977..7245
                     /locus_tag="PWU31_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1085343.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="PWU31_002899"
     CDS             7732..8196
                     /locus_tag="PWU31_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1085344.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="PWU31_002900"
     CDS             8180..8809
                     /locus_tag="PWU31_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1085345.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWU31_002901"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWU31_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL1085346.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="PWU31_002902"
     CDS             10688..11134
                     /locus_tag="PWU31_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL1085347.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="PWU31_002903"
     CDS             complement(11333..11848)
                     /locus_tag="PWU31_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1085348.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="PWU31_002904"
     CDS             11965..13167
                     /locus_tag="PWU31_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1085349.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DANJMA010000213         8097 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.084, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000213 DANJMA010000000
VERSION     DANJMA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            144..1580
                     /locus_tag="PWU31_002905"
     CDS             144..1580
                     /locus_tag="PWU31_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1085350.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1769..4216
                     /locus_tag="PWU31_002906"
     CDS             1769..4216
                     /locus_tag="PWU31_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL1085351.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4446..5513
                     /gene="rlmN"
                     /locus_tag="PWU31_002907"
     CDS             4446..5513
                     /gene="rlmN"
                     /locus_tag="PWU31_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL1085352.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5706..6809)
                     /locus_tag="PWU31_002908"
     CDS             complement(5706..6809)
                     /locus_tag="PWU31_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1085353.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7362..7910
                     /gene="aac(6')"
                     /locus_tag="PWU31_002909"
     CDS             7362..7910
                     /gene="aac(6')"
                     /locus_tag="PWU31_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL1085354.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2709 a         1380 c         1755 g         2253 t
ORIGIN      
        1 ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac aacctccttt
       61 acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg aacgattgaa
      121 ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt ccaaaagatt
      181 ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa gaagatggga
      241 aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt aaaggaacga
      301 acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct ttaatgaaag
      361 aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt cctaatggac
      421 gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac gaattgattg
      481 aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa gcattgcaag
      541 atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat tatgcagaaa
      601 cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat gaacaaaata
      661 tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca gacccgaaac
      721 gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc aataaatttc
      781 acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca aattatccaa
      841 ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg atggccaatt
      901 attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg ttcttagaag
      961 aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa tctgctaaac
     1021 cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat ccattaaacg
     1081 gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa gaaactggag
     1141 ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat tgggactggg
     1201 agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt taccgcatgc
     1261 cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa gataaacaaa
     1321 tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc aaagaagcaa
     1381 tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat ttgcttagct
     1441 ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac gaaacacaag
     1501 aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa ctgattgaag
     1561 aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct ggatatggcc
     1621 gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata agctgaaaaa
     1681 tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt ttttgtataa
     1741 tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta ctaaaaaaag
     1801 gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg
     1861 tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag gttttttagg
     1921 aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa tcctctgtct
     1981 tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt caattggtaa
     2041 aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat tgttacatgc
     2101 ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga catgggattt
     2161 gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg ggatgattgg
     2221 agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt atttagcagc
     2281 agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct atcagctgtt
     2341 aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg atcctgccaa
     2401 gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga
     2461 agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat atgagaagaa
     2521 aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac cagaaaatac
     2581 agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata tccatcctgc
     2641 gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag aaggtttttc
     2701 agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag cagaacagga
     2761 aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa ctgtggatca
     2821 aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag
     2881 tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca caaaattcga
     2941 agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag atgatatcgc
     3001 gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta aatcactgat
     3061 tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa tcatcgaagc
     3121 acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg tttctggacg
     3181 ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt cgactggtag
     3241 tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg ctaagcccca
     3301 tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt acaacggcat
     3361 acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa
     3421 agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag taagaaatat
     3481 ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga aacatccaac
     3541 cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg tagccagcaa
     3601 tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg ggattcatat
     3661 gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca aagcaaatgt
     3721 accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga tcattgattc
     3781 taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa tgggtgagaa
     3841 taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac gggtcgttca
     3901 atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga cagatgaagt
     3961 tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag caaaagcact
     4021 tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc ggatcggtta
     4081 caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg gcccgtcaga
     4141 aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa cgttgccgga
     4201 cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac caataaaaag
     4261 acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct aatacggtat
     4321 gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat ttgtgaagta
     4381 ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa
     4441 atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc aattggttta
     4501 tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac
     4561 gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg
     4621 agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag
     4681 taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag
     4741 aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt
     4801 gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga
     4861 tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg
     4921 ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata
     4981 tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg
     5041 gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt
     5101 ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc
     5161 caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca
     5221 cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag
     5281 cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag
     5341 tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg
     5401 tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg
     5461 cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc
     5521 ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga actgttcaac
     5581 ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc
     5641 ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca
     5701 attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct
     5761 acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata
     5821 tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc
     5881 atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac
     5941 aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac agatgctccg
     6001 tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata
     6061 gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc
     6121 gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc
     6181 gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc
     6241 gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca
     6301 tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg
     6361 aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta
     6421 tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag
     6481 actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg
     6541 actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt
     6601 ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa
     6661 gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact
     6721 tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt tccaatggta
     6781 taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg
     6841 ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta
     6901 agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagtatcct
     6961 aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt tcctcttagt
     7021 atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa attaagatta
     7081 ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata
     7141 atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt
     7201 cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag ataaataatg
     7261 aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc
     7321 aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg
     7381 accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg
     7441 aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg
     7501 cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg
     7561 gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa
     7621 taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga
     7681 tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg
     7741 agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct
     7801 atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac
     7861 cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg
     7921 ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga
     7981 atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc gtgtaccaga
     8041 ggttgtgaaa aagtcgctag tttcgagtag taagaaaaat tttgtgaaaa tggcttt
//
LOCUS       DANJMA010000214         5781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.095, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000214 DANJMA010000000
VERSION     DANJMA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            243..1211
                     /locus_tag="PWU31_002910"
     CDS             243..1211
                     /locus_tag="PWU31_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL1085355.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1204..2235
                     /gene="vanA"
                     /locus_tag="PWU31_002911"
     CDS             1204..2235
                     /gene="vanA"
                     /locus_tag="PWU31_002911"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL1085356.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2241..2849
                     /locus_tag="PWU31_002912"
     CDS             2241..2849
                     /locus_tag="PWU31_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL1085357.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3277..4188
                     /gene="vanY"
                     /locus_tag="PWU31_002913"
     CDS             3277..4188
                     /gene="vanY"
                     /locus_tag="PWU31_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL1085358.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4341..4826
                     /gene="vanZ"
                     /locus_tag="PWU31_002914"
     CDS             4341..4826
                     /gene="vanZ"
                     /locus_tag="PWU31_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL1085359.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNEHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5113..5442)
                     /locus_tag="PWU31_002915"
     CDS             complement(5113..5442)
                     /locus_tag="PWU31_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085360.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT         1854 a          989 c         1225 g         1713 t
ORIGIN      
        1 gctggtgtca tccaccaaca tcaaaaagta tagagccata attttttagg aaaatctcaa
       61 ggttatcttt actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt
      121 acacggtaga cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac
      181 aagattacca ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa
      241 ttatgaataa catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc
      301 atgctctttc gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat
      361 ccaacgccaa atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt
      421 ccgcctctat tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca
      481 tcggctgcaa tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg
      541 tggcgtactc gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac
      601 gcaacgtaaa atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc
      661 gtggcaaggt actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag
      721 cggttattga gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa
      781 gtatagaggt aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc
      841 ttcatgtgcc gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa
      901 tgaagcaagg agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt
      961 tggttaaagc attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag
     1021 aggaagagtt tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta
     1081 aacttcaaag aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag
     1141 cgttgcgtga taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg
     1201 agcatgaata gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta
     1261 tcggtaaaat ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta
     1321 tacattggaa ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg
     1381 gaaaacgaca attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt
     1441 gttaaaaaga accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat
     1501 ggcaagtcag gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt
     1561 gtaggctgcg atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt
     1621 gcgaaaaatg ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg
     1681 gtggcagcta cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc
     1741 ggtgtgaaaa aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa
     1801 tatgacagca aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta
     1861 ttgggaaaca gtgccgcgtt agctgttggc gaggtggacc aaatcaggct gcagtacgga
     1921 atctttcgta ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc
     1981 gttcccgcag acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata
     2041 tataaagcgc tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc
     2101 cgcattgtac tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc
     2161 cgtatgatgg ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta
     2221 gcgttaaagg ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg
     2281 gtgttcgttg ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg
     2341 gttatgaagt aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa
     2401 aagaactggc tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaatc
     2461 gtgctgtaaa ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa
     2521 gttattatcc caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat
     2581 caagccatag ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc
     2641 ttgtaccaat ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg
     2701 gaatatcatg caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg
     2761 ggtttgaagc atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca
     2821 atagctattt tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaactatata
     2881 agctaactct ttcggcagga aacccgacgt atgtaactgg ttcttaggga atttatatat
     2941 agtagatagt attgaagatg taaggcagag cgatattgcg gtcattatct gcgtgcgctg
     3001 cggcaagata gcctgataat aagactgatc gcatagaggg gtggtatttc acaccgccca
     3061 ttgtcaacag gcagttcagc ctcgttaaat tcagcatggg tatcacttat gaaaattcat
     3121 ctacattggt gataatagta aatccagtag ggcgaaataa ttgactgtaa tttacggggc
     3181 aaaacggcac aatctcaaac gagattgtgc cgtttaaggg gaagattcta gaaatatttc
     3241 atacttccaa ctatatagtt aaggaggaga ctgaaaatga agaagttgtt ttttttattg
     3301 ttattgttat tcttaatata cttaggttat gactacgtta atgaagcact gttttctcag
     3361 gaaaaagtcg aatttcaaaa ttatgatcaa aatcccaaag aacatttaga aaatagtggg
     3421 acttctgaaa atacccaaga gaaaacaatt acagaagaac aggtttatca aggaaatctg
     3481 ctattaatca atagtaaata tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat
     3541 ttatctaaac atgacgaatt aataaatgga tacgggttgc ttgatagtaa tatttatatg
     3601 tcaaaagaaa tagcacaaaa attttcagag atggtcaatg atgctgtaaa gggtggcgtt
     3661 agtcatttta ttattaatag tggctatcga gactttgatg agcaaagtgt gctttaccaa
     3721 gaaatggggg ctgagtatgc cttaccagca ggttatagtg agcataattc aggtttatca
     3781 ctagatgtag gatcaagctt gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa
     3841 gaaaatgctt ggaaatacgg gttcatttta cgttatccag aggacaaaac agagttaaca
     3901 ggaattcaat atgaactatg gcatattcgc tatgttggtt taccacatag tgcgattatg
     3961 aaagaaaaga atttcgttct cgaggaatat atggattacc taaaagaaga aaaaaccatt
     4021 tctgttagtg taaatgggga aaaatatgag atcttttatt atcctgttac taaaaatacc
     4081 accattcatg tgccgactaa tcttcgttat gagatatcag gaaacaatat agacggtgta
     4141 attgtgacag tgtttcccgg atcaacacat actaattcaa ggaggtaagg atggcggaat
     4201 gaaaccaacg aaattaatga acagcattat tgtactagca cttttggggt aacgttagct
     4261 ttttaattta aaacccacgt taactaggac attgctatac taatgataca acttaaacaa
     4321 aagaattaga ggaaattata ttgggaaaaa tattatctag aggattgcta gctttatatt
     4381 tagtgacact aatctggtta gtgttattca aattacaata caatatttta tcagtattta
     4441 attatcatca aagaagtctt aacttgactc catttactgc tactgggaat ttcagagaga
     4501 tgatagataa tgttataatc tttattccat ttggcttgct tttgaatgtc aattttaaag
     4561 aaatcggatt tttacctaag tttgcttttg tactggtttt aagtcttact tttgaaataa
     4621 ttcaatttat cttcgctatt ggagcgacag acataacaga tgtaattaca aatactgttg
     4681 gaggctttct tggactgaaa ttatatggtt taagcaatga gcatatgaat caaaaaaaat
     4741 tagacagagt tattattttt gtaggtatac ttttgctcgt attattgctc gtttaccgta
     4801 cccatttaag aataaattac gtgtaagatg tctaaatcaa gcaatctgat ctttcataca
     4861 cataaagata ttgaatgaat tggattagat ggaaaacggg atgtggggaa actcgcccgt
     4921 aggtgtgaag tgaggggaaa accggtgata aagtaaaaag cttacctaac actatagtaa
     4981 caaagaaagc ccaattatca attttagtgc tgaggaattg gtctctttaa taaatttcct
     5041 taacgttgta aatccgcatt ttcctgacgg tacccccaaa ctaagatttc atagatagat
     5101 tttctgataa ctttaaaaaa cacgaatagc ggaaaggatg tcttcattcg ctaaagagta
     5161 ataaactact tttccatctt gacgtgattt tgccattcca tgttttttta aaaaacgtaa
     5221 atggtgtgac gttgtagcga cactagcagt aagtatttct gctaaatcac agacgcacat
     5281 ttccttatag gtttctaaag catagaaaat tttaattcta gaaaaatcgc tgaaacattt
     5341 ccctaacaca agtaagtttg aaaaatcttt ttgatccaat ttatttttta ttccatttac
     5401 tttttcttta tgtacgacag tcacttggca aacttttttc attcatctca cctcacattc
     5461 aaacgattat ttgaatgaag tatacctatt tgatcactct tttgtcaatc taccttaatt
     5521 ccaatacact attataagca atttcatgta ttgaagtaaa cttacttgca agtgcgtttt
     5581 tgattccaag ttaaaaacat attattattc tacataattt cctaatcaaa taaaactcct
     5641 ttgaaaattg ttctaaagga gttttattat tttgtatttc tactaaaaat agcgtacaat
     5701 gtaattacta agtaattacg aaatgtaaaa taatggagga aatggttctg ttgcaaagtt
     5761 ttaaatctac tatcaaataa g
//
LOCUS       DANJMA010000215         4998 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.107, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000215 DANJMA010000000
VERSION     DANJMA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            complement(30..2180)
                     /locus_tag="PWU31_002916"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="PWU31_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="PWU31_002917"
     CDS             2326..2901
                     /locus_tag="PWU31_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1085361.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="PWU31_002918"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="PWU31_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL1085362.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="PWU31_002919"
     CDS             3788..4942
                     /locus_tag="PWU31_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL1085363.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1561 a          939 c          968 g         1530 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatcttag
//
LOCUS       DANJMA010000216         2993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.128, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000216 DANJMA010000000
VERSION     DANJMA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..188
                     /locus_tag="PWU31_002920"
     CDS             <1..188
                     /locus_tag="PWU31_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1085364.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="PWU31_002921"
     CDS             203..1072
                     /locus_tag="PWU31_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL1085365.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="PWU31_002922"
     CDS             1053..1787
                     /locus_tag="PWU31_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1085366.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="PWU31_002923"
     CDS             1820..2728
                     /locus_tag="PWU31_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL1085367.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="PWU31_002924"
     CDS             2725..2973
                     /locus_tag="PWU31_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HDL1085368.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DANJMA010000217         2308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.141, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000217 DANJMA010000000
VERSION     DANJMA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            46..438
                     /locus_tag="PWU31_002925"
     CDS             46..438
                     /locus_tag="PWU31_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1085369.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            439..1878
                     /locus_tag="PWU31_002926"
     CDS             439..1878
                     /locus_tag="PWU31_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL1085370.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(2008..>2308)
                     /locus_tag="PWU31_002927"
     CDS             complement(2008..>2308)
                     /locus_tag="PWU31_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1085371.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          918 a          207 c          394 g          789 t
ORIGIN      
        1 acactaaaat agattttttg gaaaactttg caacagaacc tcctattgaa actattgttt
       61 attgatgata atatggtttt tgatattgat aatttaaaag gatttcttaa tgataccagt
      121 tcatttgggt ttatagctaa agaaaataat aaaattatag gatttgcata ttgctataca
      181 cttttaagac ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac
      241 tatcaagaca aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag
      301 attggttgtt ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta
      361 tatgaaaaat taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat
      421 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      481 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      541 gatggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      601 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      661 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      721 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      781 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      841 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      901 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      961 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
     1021 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
     1081 gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac taataagaaa
     1141 aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt agaaactaat
     1201 gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat actaggttat
     1261 aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc agaagaagaa
     1321 caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg tttagattat
     1381 acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga gtatatattg
     1441 ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat agaaagtttt
     1501 atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg ccataatgat
     1561 tttagttgta atcatctatt gttagatggc aataatagat taactggaat aattgatttt
     1621 ggagattctg gaattataga tgaatattgt gattttatat acttacttga agatagtgaa
     1681 gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa tatagatatt
     1741 gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga aactattgtt
     1801 tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga aatttataaa
     1861 aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga cattagtatt
     1921 aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga ctcctgtgta
     1981 aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt ttcttgaaga
     2041 ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat tggctgatgt
     2101 ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat tcagtcgttc
     2161 aattagattg gtactcttta gtcgattgtg ggaatttcct tgtacggtat attgaaaggc
     2221 gtcttcgaat ccatcatcca atgatgcgca agcttttgaa tattttggtt ggttctgttg
     2281 caaagtttta aatctactat caaataag
//
LOCUS       DANJMA010000218         1954 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.152, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000218 DANJMA010000000
VERSION     DANJMA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            <1..597
                     /locus_tag="PWU31_002928"
     CDS             <1..597
                     /locus_tag="PWU31_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HDL1085372.1"
                     /translation="PYLIVDLKLCNRTDNLIRGEFHFLSEKDMNIIPSFKDSGYIPDT
                     KAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGINVLKRGEYARS
                     LELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKTTARLDKVELFE
                     AYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            1285..1954
                     /locus_tag="PWU31_002929"
                     /pseudo
     CDS             1285..1954
                     /locus_tag="PWU31_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
BASE COUNT          658 a          315 c          402 g          579 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg ataatcttat acgtggggaa
       61 tttcatttcc tttctgaaaa agatatgaac ataatcccct cgtttaaaga ttcaggttat
      121 attcctgata cgaaggctat gcttatttac gatgaaacag ggcaattaga aaattattta
      181 tcagagataa gtggtgcaag accaaataga cttactgaag aaaatgctaa ttttttgttg
      241 tgtaatttct ctaatctatg gttgatggga atcaacgttc taaaaagagg agaatatgct
      301 cgttcattag aactcttatc acaacttcaa aaaaatacac tacaacttat acgtatggca
      361 gaaaaaaatg ctgataattg gctaaacatg agtaaaaacc ttgaaaaaga aattagcctt
      421 gaaaattata aaaaatttgc aaagaccact gctcgattag ataaggtaga attatttgaa
      481 gcctataaaa attctttgct attagttatg gatttgcaaa gtcaccttat tgaacaatac
      541 aacttaaaag ttacacatga cattttagaa agattgttga attacattag tgaataggag
      601 agtaacgtta ttcacagtat tgggtactga aaattaacag catacctcag tttttcaaaa
      661 aacagcgaag cctatggata tgttcccgtc taccgatagt gcaaaaagtg tcgtggatat
      721 gtttccgaga acggacatac acaaatgaca ttcattctgt cctctcaatc catgtggaag
      781 cagtatgtct gttaacaaga aaataattat tgttcgtcct gacgagttgt tagactcgaa
      841 aaggataaac atcattttta gaaccggtag ccgatttttg gctagcggtt ttttgggctc
      901 tttggcaaat agtggtgatt gaatctcgcg ctatcctatg gaaaaccgcc ttgtgtgagg
      961 ggctgcgaga ccgcttcgat cctagaattg ctgaagcacg aatctagcgt cactcacatt
     1021 tcatcttttg acgtagctga gacaaggtgt ttttcttcga tccgctagtg gttaatctct
     1081 ttttggtgca aatatggttt agaatttctt tgcatggctt aagggagtgg agaatcggat
     1141 attagtagga tttacatcca cagataaaaa taggcttgat tttaacagcg aaactaaaat
     1201 accctactta aatgaagtga tagattagat gttgggttgt taggagatgc ttaaaaatcc
     1261 tatgcaatca atagttttta gtgtttgttg acttgatccc taaatcgcct gagtactaaa
     1321 cgttgagtgt gtctgcttac tgtcactagc cagtaagtga gactgtagtt aatctctcat
     1381 tttccccagt attatgttga aaatagccat gaagcgatta tttctaaaga gttatttttg
     1441 caggctcaag aagaaattta tcggagaagt aatatctaca caggagaagg caagaacaaa
     1501 agaatttata gtagcaagta cgctttaagt gccatcacct tctgtggata ttgtggcgat
     1561 atttatagga gaacctattg gaatattcat ggcagaaaag aatttgtctg gcgatgcgtg
     1621 actagaatcg agcagggtcc cgaggtatgt aagaaccgaa ccgtaaaaga agatgaactc
     1681 tatggtgcgg taatgaccgc aattaataag ctacttgctg gtggcaacaa tatgataaag
     1741 acactggaag aaaatattca tgctgtgatt ggagaaacca cagaatacca aatttcagag
     1801 attaataccc tactagatga aaaacaaaaa gaactaatca agctggccaa taaaggtcaa
     1861 gattatgaat acctggtaga tgagatcgat gagatgcgag ataagcgtca gactctttta
     1921 gtagaagatg cctccctcag tggcgagaac gagc
//
LOCUS       DANJMA010000219         1504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.164, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000219 DANJMA010000000
VERSION     DANJMA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            262..345
                     /locus_tag="PWU31_002930"
                     /pseudo
     CDS             262..345
                     /locus_tag="PWU31_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="PWU31_002931"
     CDS             394..477
                     /locus_tag="PWU31_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL1085373.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="PWU31_002932"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="PWU31_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL1085374.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DANJMA010000220         1349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538668-rid17232773.guided.178, whole genome shotgun
            sequence.
ACCESSION   DANJMA010000220 DANJMA010000000
VERSION     DANJMA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538668
            Sequence Read Archive: ERR10688231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637967.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637967.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:31:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 33 of 76
            Pseudo Genes (internal stop)      :: 27 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538668-rid17232773.guided.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-08-27"
     gene            46..297
                     /locus_tag="PWU31_002933"
                     /pseudo
     CDS             46..297
                     /locus_tag="PWU31_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="PWU31_002934"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="PWU31_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL1085375.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
